AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021164

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021164.t1 MTR_4g064887 94.007 267 16 0 1 267 1 267 0.0 515
MS.gene021164.t1 MTR_4g064887 92.344 209 16 0 1 209 1 209 6.96e-143 400
MS.gene021164.t1 MTR_3g068155 61.004 259 101 0 3 261 7 265 3.44e-121 347
MS.gene021164.t1 MTR_3g068070 59.160 262 107 0 1 262 1 262 2.26e-118 340
MS.gene021164.t1 MTR_3g068175 62.302 252 95 0 11 262 13 264 1.28e-117 338
MS.gene021164.t1 MTR_4g064883 62.451 253 95 0 10 262 9 261 1.14e-115 333
MS.gene021164.t1 MTR_3g068175 61.328 256 95 1 11 262 13 268 3.26e-115 332
MS.gene021164.t1 MTR_3g068085 59.274 248 101 0 15 262 15 262 1.67e-111 322
MS.gene021164.t1 MTR_3g068065 59.677 248 100 0 15 262 17 264 2.31e-110 320
MS.gene021164.t1 MTR_7g063270 56.818 264 110 2 1 260 1 264 4.93e-109 316
MS.gene021164.t1 MTR_7g063390 55.805 267 111 3 1 261 1 266 3.53e-105 306
MS.gene021164.t1 MTR_7g063260 55.094 265 114 2 1 260 1 265 3.74e-104 303
MS.gene021164.t1 MTR_7g063360 54.135 266 117 3 1 261 1 266 3.89e-101 296
MS.gene021164.t1 MTR_7g063320 53.992 263 117 2 1 259 1 263 3.06e-100 293
MS.gene021164.t1 MTR_7g063330 53.232 263 120 1 1 260 1 263 1.94e-99 291
MS.gene021164.t1 MTR_4g064883 62.000 200 76 0 10 209 9 208 5.71e-87 258
MS.gene021164.t1 MTR_7g063270 49.242 264 81 4 1 260 1 215 1.90e-82 246
MS.gene021164.t1 MTR_4g132850 32.927 246 160 4 15 259 7 248 4.33e-33 121
MS.gene021164.t1 MTR_3g085740 33.740 246 152 7 21 261 81 320 8.11e-30 114
MS.gene021164.t1 MTR_7g094510 32.400 250 161 5 15 258 6 253 2.26e-27 106
MS.gene021164.t1 MTR_8g442750 31.008 258 159 6 15 259 14 265 2.09e-25 102
MS.gene021164.t1 MTR_7g094520 29.880 251 168 4 15 259 11 259 2.87e-25 101
MS.gene021164.t1 MTR_4g131740 29.434 265 175 6 8 263 33 294 1.12e-24 100
MS.gene021164.t1 MTR_4g058840 28.346 254 175 5 15 261 11 264 1.36e-21 91.7
MS.gene021164.t1 MTR_4g058820 28.346 254 175 5 15 261 11 264 1.36e-21 91.7
MS.gene021164.t1 MTR_4g052350 31.128 257 156 11 15 261 14 259 1.89e-21 91.3
MS.gene021164.t1 MTR_4g058810 27.953 254 176 5 15 261 11 264 3.42e-21 90.5
MS.gene021164.t1 MTR_1g051165 25.769 260 179 6 15 261 14 272 4.45e-20 87.4
MS.gene021164.t1 MTR_7g113650 29.572 257 170 6 15 263 40 293 8.02e-20 87.0
MS.gene021164.t1 MTR_6g023990 28.195 266 180 7 1 259 1 262 8.62e-20 86.7
MS.gene021164.t1 MTR_1196s0010 30.328 244 155 9 20 258 279 512 9.89e-20 88.6
MS.gene021164.t1 MTR_6g023610 28.679 265 175 7 1 257 1 259 1.06e-19 86.3
MS.gene021164.t1 MTR_4g052340 29.811 265 151 12 15 262 14 260 1.46e-19 85.9
MS.gene021164.t1 MTR_3g020670 30.370 270 169 10 5 260 6 270 2.68e-19 85.5
MS.gene021164.t1 MTR_7g105460 28.519 270 175 9 1 261 1 261 2.80e-19 85.1
MS.gene021164.t1 MTR_4g052320 30.418 263 161 9 10 261 3 254 3.50e-19 84.7
MS.gene021164.t1 MTR_7g071680 27.491 291 173 8 6 266 20 302 4.84e-19 85.1
MS.gene021164.t1 MTR_1g068955 31.907 257 152 8 15 257 14 261 5.50e-19 84.7
MS.gene021164.t1 MTR_6g023590 28.571 266 178 7 1 259 1 261 7.36e-19 84.0
MS.gene021164.t1 MTR_1g082760 28.516 256 172 6 15 262 40 292 1.79e-18 83.2
MS.gene021164.t1 MTR_2g090165 28.195 266 171 9 11 264 2 259 1.00e-17 80.9
MS.gene021164.t1 MTR_6g023600 28.947 266 177 7 1 259 1 261 1.07e-17 80.9
MS.gene021164.t1 MTR_1579s0020 43.750 80 44 1 181 260 1 79 1.66e-17 75.9
MS.gene021164.t1 MTR_4g052300 27.132 258 175 6 10 261 3 253 4.61e-17 79.0
MS.gene021164.t1 MTR_6g023910 27.273 264 180 7 1 257 1 259 4.99e-17 79.0
MS.gene021164.t1 MTR_4g078630 27.857 280 162 12 5 260 8 271 2.22e-16 77.4
MS.gene021164.t1 MTR_7g083870 26.996 263 165 6 15 260 35 287 5.19e-16 76.6
MS.gene021164.t1 MTR_6g023950 28.195 266 179 7 1 259 1 261 6.29e-16 75.9
MS.gene021164.t1 MTR_7g110160 28.125 256 158 7 11 261 2 236 6.61e-16 75.5
MS.gene021164.t1 MTR_6g023600 28.195 266 173 7 1 259 1 255 1.61e-15 74.7
MS.gene021164.t1 MTR_7g110130 26.641 259 177 8 11 261 2 255 3.32e-15 73.9
MS.gene021164.t1 MTR_7g080410 28.646 192 119 4 22 202 45 229 4.68e-15 73.9
MS.gene021164.t1 MTR_7g080410 28.646 192 119 4 22 202 45 229 4.90e-15 73.6
MS.gene021164.t1 MTR_7g107670 25.283 265 180 6 8 260 26 284 5.56e-15 73.6
MS.gene021164.t1 MTR_7g105570 29.944 177 111 5 94 261 46 218 7.78e-14 69.3
MS.gene021164.t1 MTR_4g058830 27.922 154 107 3 112 261 57 210 1.58e-13 68.2
MS.gene021164.t1 MTR_8g089575 28.704 216 143 6 17 225 47 258 3.68e-13 68.6
MS.gene021164.t1 MTR_6g041980 29.365 252 152 8 15 250 64 305 3.78e-13 68.9
MS.gene021164.t1 MTR_7g110140 28.016 257 175 8 11 261 2 254 7.55e-13 67.0
MS.gene021164.t1 MTR_7g105590 26.596 188 133 4 15 200 8 192 3.55e-12 64.3
MS.gene021164.t1 MTR_7g105580 24.715 263 161 8 15 265 8 245 5.34e-12 64.7
MS.gene021164.t1 MTR_6g041980 32.680 153 97 4 5 154 54 203 1.53e-11 63.5
MS.gene021164.t1 MTR_7g053290 31.148 183 117 7 82 259 192 370 2.85e-11 63.5
MS.gene021164.t1 MTR_7g076655 26.939 245 120 6 20 208 9 250 7.25e-11 61.6
MS.gene021164.t1 MTR_6g035395 25.882 255 168 7 12 261 2 240 7.82e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021164.t1 AT1G07440 63.602 261 94 1 7 267 4 263 1.21e-122 350
MS.gene021164.t1 AT2G29150 63.878 263 93 2 1 262 1 262 3.56e-119 342
MS.gene021164.t1 AT2G29340 61.628 258 99 0 10 267 2 259 4.68e-119 341
MS.gene021164.t1 AT5G06060 61.265 253 98 0 10 262 4 256 5.02e-119 341
MS.gene021164.t1 AT2G29340 61.628 258 99 0 10 267 2 259 2.27e-118 341
MS.gene021164.t1 AT2G29350 63.740 262 94 1 1 262 1 261 4.56e-118 339
MS.gene021164.t1 AT2G29290 62.791 258 96 0 10 267 2 259 5.28e-117 336
MS.gene021164.t1 AT2G29360 63.878 263 94 1 1 262 1 263 8.97e-117 336
MS.gene021164.t1 AT2G30670 61.265 253 98 0 10 262 2 254 1.70e-114 330
MS.gene021164.t1 AT2G29310 60.000 255 102 0 10 264 2 256 1.76e-112 325
MS.gene021164.t1 AT1G07450 63.347 251 91 1 12 262 5 254 7.53e-111 320
MS.gene021164.t1 AT2G29370 61.217 263 101 1 1 262 1 263 9.59e-111 320
MS.gene021164.t1 AT2G29260 59.363 251 102 0 12 262 65 315 1.22e-109 320
MS.gene021164.t1 AT2G29330 57.752 258 109 0 10 267 2 259 2.23e-109 317
MS.gene021164.t1 AT2G29320 58.203 256 106 1 13 267 11 266 1.14e-107 313
MS.gene021164.t1 AT2G29300 57.708 253 106 1 10 261 2 254 5.09e-106 308
MS.gene021164.t1 AT2G29300 57.540 252 106 1 10 260 2 253 1.29e-104 305
MS.gene021164.t1 AT2G29290 63.478 230 84 0 33 262 8 237 1.66e-104 303
MS.gene021164.t1 AT2G29350 63.677 223 80 1 40 262 2 223 2.65e-100 292
MS.gene021164.t1 AT2G29310 59.914 232 93 0 31 262 21 252 2.00e-99 291
MS.gene021164.t1 AT2G29320 56.723 238 102 1 31 267 11 248 7.01e-96 282
MS.gene021164.t1 AT2G29350 63.942 208 75 0 1 208 1 208 7.62e-92 271
MS.gene021164.t1 AT2G29340 58.500 200 83 0 10 209 2 201 5.15e-86 255
MS.gene021164.t1 AT2G29340 58.500 200 83 0 10 209 2 201 5.15e-86 255
MS.gene021164.t1 AT2G29310 56.500 200 87 0 10 209 2 201 2.18e-81 243
MS.gene021164.t1 AT2G29320 56.545 191 82 1 13 202 11 201 1.34e-75 229
MS.gene021164.t1 AT1G07440 60.843 166 65 0 7 172 4 169 1.61e-71 217
MS.gene021164.t1 AT2G29310 55.307 179 80 0 31 209 21 199 2.41e-68 210
MS.gene021164.t1 AT2G29170 61.765 102 38 1 1 101 1 102 5.43e-38 129
MS.gene021164.t1 AT4G05530 31.707 246 163 4 15 259 9 250 2.89e-30 114
MS.gene021164.t1 AT3G03980 31.852 270 163 7 5 258 4 268 1.56e-28 110
MS.gene021164.t1 AT1G24360 32.787 244 157 6 21 261 80 319 1.30e-27 108
MS.gene021164.t1 AT3G55310 30.078 256 168 7 15 261 17 270 3.27e-27 106
MS.gene021164.t1 AT3G04000 32.075 265 160 8 15 265 14 272 3.65e-27 106
MS.gene021164.t1 AT4G13180 31.179 263 173 6 5 261 3 263 8.20e-27 105
MS.gene021164.t1 AT3G55290 29.297 256 170 7 15 261 17 270 9.36e-26 102
MS.gene021164.t1 AT3G55290 29.297 256 170 7 15 261 18 271 9.63e-26 102
MS.gene021164.t1 AT1G63380 30.739 257 166 7 15 261 17 271 1.13e-25 102
MS.gene021164.t1 AT1G63380 30.739 257 166 7 15 261 19 273 1.20e-25 102
MS.gene021164.t1 AT1G63380 30.739 257 166 7 15 261 15 269 1.33e-25 102
MS.gene021164.t1 AT5G18210 30.268 261 159 6 14 259 7 259 1.83e-25 101
MS.gene021164.t1 AT3G05260 33.083 266 164 8 7 262 27 288 1.91e-25 102
MS.gene021164.t1 AT1G63380 30.739 257 166 7 15 261 22 276 1.92e-25 102
MS.gene021164.t1 AT3G12800 28.346 254 172 4 15 259 10 262 1.61e-24 100
MS.gene021164.t1 AT1G54870 32.443 262 154 10 15 262 82 334 1.66e-24 100
MS.gene021164.t1 AT1G54870 32.443 262 154 10 15 262 84 336 1.87e-24 100
MS.gene021164.t1 AT1G62610 29.961 257 168 7 15 261 19 273 3.49e-24 98.6
MS.gene021164.t1 AT1G62610 29.961 257 168 7 15 261 15 269 3.76e-24 98.6
MS.gene021164.t1 AT1G62610 29.961 257 168 7 15 261 14 268 3.77e-24 98.6
MS.gene021164.t1 AT1G62610 29.961 257 168 7 15 261 13 267 3.78e-24 98.6
MS.gene021164.t1 AT5G18210 30.453 243 146 6 14 241 7 241 2.21e-22 94.0
MS.gene021164.t1 AT3G46170 27.344 256 175 7 15 261 26 279 5.13e-21 90.1
MS.gene021164.t1 AT2G17845 27.734 256 171 7 15 258 47 300 7.36e-21 90.1
MS.gene021164.t1 AT2G47120 27.907 258 172 9 11 261 2 252 1.17e-19 85.9
MS.gene021164.t1 AT2G47120 27.953 254 169 9 15 261 7 253 1.86e-19 85.5
MS.gene021164.t1 AT3G51680 27.586 290 180 11 1 266 20 303 2.81e-18 82.8
MS.gene021164.t1 AT3G42960 29.213 267 165 9 10 260 3 261 9.03e-18 81.3
MS.gene021164.t1 AT3G26760 28.044 271 170 9 5 260 30 290 3.16e-17 80.1
MS.gene021164.t1 AT4G03140 25.781 256 176 6 15 260 78 329 8.60e-17 79.3
MS.gene021164.t1 AT1G52340 27.473 273 156 8 15 260 18 275 1.70e-15 75.1
MS.gene021164.t1 AT2G47130 26.562 256 179 7 11 261 2 253 4.01e-15 73.6
MS.gene021164.t1 AT3G29260 27.027 259 174 6 11 261 2 253 5.49e-15 73.2
MS.gene021164.t1 AT3G06060 28.571 217 144 5 9 220 31 241 1.06e-14 73.2
MS.gene021164.t1 AT3G06060 28.571 217 144 5 9 220 48 258 1.34e-14 73.2
MS.gene021164.t1 AT3G26770 27.273 264 163 8 15 260 41 293 1.26e-13 69.7
MS.gene021164.t1 AT5G19200 27.619 210 143 4 15 220 35 239 3.30e-13 68.9
MS.gene021164.t1 AT1G10310 31.551 187 124 4 19 204 19 202 2.57e-12 65.5
MS.gene021164.t1 AT3G03330 26.531 196 135 5 14 202 40 233 3.28e-12 65.9
MS.gene021164.t1 AT5G65205 26.596 188 127 5 21 205 12 191 5.51e-11 62.0
MS.gene021164.t1 AT3G29250 26.894 264 177 9 11 265 2 258 6.01e-11 61.6
MS.gene021164.t1 AT3G29250 26.923 260 174 9 15 265 44 296 9.28e-11 61.6
MS.gene021164.t1 AT3G29250 26.923 260 174 9 15 265 42 294 9.71e-11 61.6

Find 63 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCAAGATTTCTCATTTC+TGG 0.136218 4.4:-41545022 MS.gene021164:CDS
CAAGCCCAGCTTATTGTAGA+AGG 0.344955 4.4:+41544091 None:intergenic
GTAAAATCTAGTGATTGTTT+TGG 0.345117 4.4:+41545045 None:intergenic
CCTGCAGCCTCTTTCATAAC+AGG 0.358569 4.4:-41544156 MS.gene021164:CDS
ACTGGTGGATCCAAAGGAAT+AGG 0.388939 4.4:-41545870 MS.gene021164:CDS
GGACAGACCATTTGTATTGA+TGG 0.394810 4.4:-41544135 MS.gene021164:CDS
AGAACCCCTCTTGGACGTAT+TGG 0.397142 4.4:-41544222 MS.gene021164:CDS
AGATAGCAACCCTATTCCTT+TGG 0.397711 4.4:+41545860 None:intergenic
TCAATGAATGCTTACATCAA+TGG 0.398769 4.4:-41545324 MS.gene021164:CDS
TTCATTTCAAGAACCCCTCT+TGG 0.404796 4.4:-41544231 MS.gene021164:CDS
GCAGCAAGAGGGGTTCTGAT+TGG 0.447876 4.4:+41544668 None:intergenic
CAATGAATGCTTACATCAAT+GGG 0.449372 4.4:-41545323 MS.gene021164:CDS
ACCAAGGAAGACACCTCTTC+TGG 0.466532 4.4:+41544195 None:intergenic
GAGTTTGAAGCTTTGAGTAA+TGG 0.471691 4.4:+41544952 None:intergenic
TATGACTAGGTATGATATAG+TGG 0.472016 4.4:-41545404 MS.gene021164:intron
AGAGGGGTTCTGATTGGCCC+TGG 0.476277 4.4:+41544674 None:intergenic
AAATAGTAAGTGGTCTTTGA+AGG 0.483818 4.4:-41545910 MS.gene021164:CDS
ACCAGAAGAGGTGTCTTCCT+TGG 0.491068 4.4:-41544196 MS.gene021164:CDS
TGGAGCAATTAGCAGAGCTA+GGG 0.491202 4.4:-41545384 MS.gene021164:CDS
ATGACAGCTCTGGTCACTGG+TGG 0.504668 4.4:-41545885 MS.gene021164:CDS
ACCATCTATAGTGCAGCAAA+AGG 0.506661 4.4:-41544874 MS.gene021164:intron
TTTGAAGGGGATGACAGCTC+TGG 0.513224 4.4:-41545895 MS.gene021164:CDS
GGGATGACAGCTCTGGTCAC+TGG 0.516153 4.4:-41545888 MS.gene021164:CDS
TGAGTAATGGATGTGCAAGC+TGG 0.522910 4.4:+41544965 None:intergenic
AATAGTAAGTGGTCTTTGAA+GGG 0.525324 4.4:-41545909 MS.gene021164:CDS
GTGGAGCAATTAGCAGAGCT+AGG 0.526426 4.4:-41545385 MS.gene021164:CDS
AGGACTAATTGTGTTGCACC+AGG 0.529569 4.4:-41544692 MS.gene021164:CDS
TTACATCAATGGGTCACAAA+AGG 0.532043 4.4:-41545313 MS.gene021164:CDS
AGACCATTTGTATTGATGGC+GGG 0.533740 4.4:-41544131 MS.gene021164:CDS
CAAGTAGCAGAAATAGTAAG+TGG 0.538795 4.4:-41545920 MS.gene021164:CDS
CTGGTCACTGGTGGATCCAA+AGG 0.539538 4.4:-41545876 MS.gene021164:CDS
TAACCCGCCATCAATACAAA+TGG 0.540084 4.4:+41544128 None:intergenic
AGAGTCTCTTCTGAGTTCAA+TGG 0.543714 4.4:-41545226 MS.gene021164:CDS
GGGGTAGCATCACTAAATGT+AGG 0.544472 4.4:-41544898 MS.gene021164:CDS
ACAAAAGGATACAAAATCAC+TGG 0.547408 4.4:-41545298 MS.gene021164:CDS
CCTGTTATGAAAGAGGCTGC+AGG 0.552564 4.4:+41544156 None:intergenic
CTGGTGGATCCAAAGGAATA+GGG 0.554228 4.4:-41545869 MS.gene021164:intron
CCACTGTACACACTTGTGCT+AGG 0.562221 4.4:-41545360 MS.gene021164:CDS
TTTCATGTCCTCCATTGTTG+GGG 0.575336 4.4:-41544917 MS.gene021164:CDS
GAAATTTAGGTGAACAATGT+GGG 0.576001 4.4:-41545078 MS.gene021164:intron
AAATTACTTCTGCAGCAAGA+GGG 0.582091 4.4:+41544657 None:intergenic
AATGGGCTAGAGACAACATA+AGG 0.589389 4.4:-41544712 MS.gene021164:CDS
GTTCTCCAATACGTCCAAGA+GGG 0.595929 4.4:+41544217 None:intergenic
AAAATTACTTCTGCAGCAAG+AGG 0.596709 4.4:+41544656 None:intergenic
AGAAGAGGTGTCTTCCTTGG+TGG 0.599777 4.4:-41544193 MS.gene021164:CDS
AAGAGGCTGCAGGTAAGCAT+AGG 0.600954 4.4:+41544166 None:intergenic
CAGACCATTTGTATTGATGG+CGG 0.601299 4.4:-41544132 MS.gene021164:CDS
TGAAATTTAGGTGAACAATG+TGG 0.603162 4.4:-41545079 MS.gene021164:intron
GTCATCTCGTGCACAAAGAG+AGG 0.604943 4.4:-41545260 MS.gene021164:CDS
AATTACTTCTGCAGCAAGAG+GGG 0.609401 4.4:+41544658 None:intergenic
ATAGTAAGTGGTCTTTGAAG+GGG 0.610704 4.4:-41545908 MS.gene021164:CDS
GGTCTGTCCTGTTATGAAAG+AGG 0.618109 4.4:+41544149 None:intergenic
TAGTGATGCTACCCCAACAA+TGG 0.638650 4.4:+41544906 None:intergenic
TATTAAGGAGAAATGGCAGA+GGG 0.641387 4.4:-41545948 None:intergenic
ACGTATTGGAGAACCAGAAG+AGG 0.658963 4.4:-41544208 MS.gene021164:CDS
GTATTAAGGAGAAATGGCAG+AGG 0.659922 4.4:-41545949 None:intergenic
GGAGCAATTAGCAGAGCTAG+GGG 0.662491 4.4:-41545383 MS.gene021164:CDS
TTCTCCAATACGTCCAAGAG+GGG 0.662521 4.4:+41544218 None:intergenic
TGATGCTACCCCAACAATGG+AGG 0.664800 4.4:+41544909 None:intergenic
GGACTAATTGTGTTGCACCA+GGG 0.676221 4.4:-41544691 MS.gene021164:CDS
TAAGCATAGGAATGCCACCA+AGG 0.681785 4.4:+41544179 None:intergenic
GGTTCTCCAATACGTCCAAG+AGG 0.697030 4.4:+41544216 None:intergenic
CCTAGCACAAGTGTGTACAG+TGG 0.731390 4.4:+41545360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAAATAAATAAATGT+CGG + chr4.4:41544939-41544958 None:intergenic 10.0%
!! TTTATTTATGTATGAAATTT+AGG - chr4.4:41544946-41544965 MS.gene021164:CDS 10.0%
!! ACTATATAAATCCTATAAAA+AGG + chr4.4:41544903-41544922 None:intergenic 15.0%
!! ATCAATTAACTACAAAATTT+GGG + chr4.4:41544354-41544373 None:intergenic 15.0%
!! CATATTTATTGAATGAAATA+TGG + chr4.4:41545711-41545730 None:intergenic 15.0%
!!! TATTTTTTTCACCTTTTTAT+AGG - chr4.4:41544889-41544908 MS.gene021164:CDS 15.0%
!! ATATATGTAATTTACAATGC+AGG - chr4.4:41545267-41545286 MS.gene021164:CDS 20.0%
!! CTAAAAACGAGTTTATAAAT+GGG + chr4.4:41545687-41545706 None:intergenic 20.0%
!! GATCAATTAACTACAAAATT+TGG + chr4.4:41544355-41544374 None:intergenic 20.0%
!! TGTATTATTAATTGCTTACA+AGG + chr4.4:41544849-41544868 None:intergenic 20.0%
!! TTGTTAAAATATTGTTCCAT+TGG + chr4.4:41544537-41544556 None:intergenic 20.0%
!!! AATTAGCTTTGAGTTAATTT+AGG + chr4.4:41544411-41544430 None:intergenic 20.0%
!!! TATTGACTAATAATAACCAA+TGG - chr4.4:41544518-41544537 MS.gene021164:intron 20.0%
! AAAAAATTTGGCATGTGAAT+GGG - chr4.4:41545308-41545327 MS.gene021164:CDS 25.0%
! AAACCAGCTTACAAAAAATT+TGG - chr4.4:41545296-41545315 MS.gene021164:CDS 25.0%
! ATGTGAATTAAACTATGACT+AGG - chr4.4:41544620-41544639 MS.gene021164:intron 25.0%
! CCTAAAAACGAGTTTATAAA+TGG + chr4.4:41545688-41545707 None:intergenic 25.0%
! TCAACTAATTTCATGATCTA+TGG + chr4.4:41544292-41544311 None:intergenic 25.0%
! TTTCTCATCAAATATGTACA+TGG + chr4.4:41545484-41545503 None:intergenic 25.0%
!! GTAAAATCTAGTGATTGTTT+TGG + chr4.4:41544995-41545014 None:intergenic 25.0%
!!! CCATTTATAAACTCGTTTTT+AGG - chr4.4:41545685-41545704 MS.gene021164:intron 25.0%
!!! TGGACACAATCTAAATTTTT+TGG + chr4.4:41545618-41545637 None:intergenic 25.0%
AAATAGTAAGTGGTCTTTGA+AGG - chr4.4:41544127-41544146 MS.gene021164:CDS 30.0%
AATAGTAAGTGGTCTTTGAA+GGG - chr4.4:41544128-41544147 MS.gene021164:CDS 30.0%
ACAAAAGGATACAAAATCAC+TGG - chr4.4:41544739-41544758 MS.gene021164:CDS 30.0%
CAAAAAATTTGGCATGTGAA+TGG - chr4.4:41545307-41545326 MS.gene021164:CDS 30.0%
CAATGAATGCTTACATCAAT+GGG - chr4.4:41544714-41544733 MS.gene021164:CDS 30.0%
GAAATTTAGGTGAACAATGT+GGG - chr4.4:41544959-41544978 MS.gene021164:CDS 30.0%
GAATTAGACTATTGACAACT+AGG + chr4.4:41544435-41544454 None:intergenic 30.0%
TATGACTAGGTATGATATAG+TGG - chr4.4:41544633-41544652 MS.gene021164:intron 30.0%
TCAATGAATGCTTACATCAA+TGG - chr4.4:41544713-41544732 MS.gene021164:CDS 30.0%
TGAAATTTAGGTGAACAATG+TGG - chr4.4:41544958-41544977 MS.gene021164:CDS 30.0%
!!! ATCTTTTAAGATCTGCAAGA+AGG + chr4.4:41545768-41545787 None:intergenic 30.0%
!!! ATGCCAAATTTTTTGTAAGC+TGG + chr4.4:41545302-41545321 None:intergenic 30.0%
!!! TAGTGATTGTTTTGGTATGT+TGG + chr4.4:41544987-41545006 None:intergenic 30.0%
AAAACTAGTGTGTTCGATGT+GGG - chr4.4:41545564-41545583 MS.gene021164:intron 35.0%
AAAATTACTTCTGCAGCAAG+AGG + chr4.4:41545384-41545403 None:intergenic 35.0%
AAATTACTTCTGCAGCAAGA+GGG + chr4.4:41545383-41545402 None:intergenic 35.0%
AGCAAAAGGTAACTGTTTCA+TGG - chr4.4:41545177-41545196 MS.gene021164:intron 35.0%
CAAGTAGCAGAAATAGTAAG+TGG - chr4.4:41544117-41544136 MS.gene021164:CDS 35.0%
CAATAGTCATGATGCTTGTT+TGG - chr4.4:41544462-41544481 MS.gene021164:intron 35.0%
GCACAAAAGAATAAGAGAAG+AGG + chr4.4:41544214-41544233 None:intergenic 35.0%
TAAAACTAGTGTGTTCGATG+TGG - chr4.4:41545563-41545582 MS.gene021164:intron 35.0%
TGAGCAAGATTTCTCATTTC+TGG - chr4.4:41545015-41545034 MS.gene021164:CDS 35.0%
TGGTATCTGACTAATGAGTT+GGG + chr4.4:41545598-41545617 None:intergenic 35.0%
TTACATCAATGGGTCACAAA+AGG - chr4.4:41544724-41544743 MS.gene021164:CDS 35.0%
TTGGTATCTGACTAATGAGT+TGG + chr4.4:41545599-41545618 None:intergenic 35.0%
! AAGCATCATGACTATTGACT+AGG + chr4.4:41544459-41544478 None:intergenic 35.0%
! ATAGTAAGTGGTCTTTGAAG+GGG - chr4.4:41544129-41544148 MS.gene021164:CDS 35.0%
! GCAGTATTAGCCAATTTTCT+AGG + chr4.4:41545653-41545672 None:intergenic 35.0%
! TTTTCATGTCCTCCATTGTT+GGG - chr4.4:41545119-41545138 MS.gene021164:intron 35.0%
!! GAGTTTGAAGCTTTGAGTAA+TGG + chr4.4:41545088-41545107 None:intergenic 35.0%
!! TTAGCCAATTTTCTAGGAGT+TGG + chr4.4:41545647-41545666 None:intergenic 35.0%
AATTACTTCTGCAGCAAGAG+GGG + chr4.4:41545382-41545401 None:intergenic 40.0%
ACCATCTATAGTGCAGCAAA+AGG - chr4.4:41545163-41545182 MS.gene021164:intron 40.0%
AGAGTCTCTTCTGAGTTCAA+TGG - chr4.4:41544811-41544830 MS.gene021164:intron 40.0%
AGATAGCAACCCTATTCCTT+TGG + chr4.4:41544180-41544199 None:intergenic 40.0%
CCATCCAACTCCTAGAAAAT+TGG - chr4.4:41545640-41545659 MS.gene021164:intron 40.0%
GGACAGACCATTTGTATTGA+TGG - chr4.4:41545902-41545921 MS.gene021164:CDS 40.0%
TAACCCGCCATCAATACAAA+TGG + chr4.4:41545912-41545931 None:intergenic 40.0%
TTCATTTCAAGAACCCCTCT+TGG - chr4.4:41545806-41545825 MS.gene021164:intron 40.0%
TTTCATGTCCTCCATTGTTG+GGG - chr4.4:41545120-41545139 MS.gene021164:intron 40.0%
! ACCTTTTGCTGCACTATAGA+TGG + chr4.4:41545167-41545186 None:intergenic 40.0%
! AGACCATTTGTATTGATGGC+GGG - chr4.4:41545906-41545925 MS.gene021164:CDS 40.0%
! CAGACCATTTGTATTGATGG+CGG - chr4.4:41545905-41545924 MS.gene021164:CDS 40.0%
! GTTTTCATGTCCTCCATTGT+TGG - chr4.4:41545118-41545137 MS.gene021164:intron 40.0%
! TTTCTAGGAGTTGGATGGAT+TGG + chr4.4:41545638-41545657 None:intergenic 40.0%
!! AATGGGCTAGAGACAACATA+AGG - chr4.4:41545325-41545344 MS.gene021164:CDS 40.0%
!! CCAATTTTCTAGGAGTTGGA+TGG + chr4.4:41545643-41545662 None:intergenic 40.0%
ACGTATTGGAGAACCAGAAG+AGG - chr4.4:41545829-41545848 MS.gene021164:intron 45.0%
AGGACTAATTGTGTTGCACC+AGG - chr4.4:41545345-41545364 MS.gene021164:CDS 45.0%
GGACTAATTGTGTTGCACCA+GGG - chr4.4:41545346-41545365 MS.gene021164:CDS 45.0%
GGGGTAGCATCACTAAATGT+AGG - chr4.4:41545139-41545158 MS.gene021164:intron 45.0%
GGTCTGTCCTGTTATGAAAG+AGG + chr4.4:41545891-41545910 None:intergenic 45.0%
GTTCTCCAATACGTCCAAGA+GGG + chr4.4:41545823-41545842 None:intergenic 45.0%
TAAGCATAGGAATGCCACCA+AGG + chr4.4:41545861-41545880 None:intergenic 45.0%
TAGTGATGCTACCCCAACAA+TGG + chr4.4:41545134-41545153 None:intergenic 45.0%
TGGAGCAATTAGCAGAGCTA+GGG - chr4.4:41544653-41544672 MS.gene021164:intron 45.0%
TTCTCCAATACGTCCAAGAG+GGG + chr4.4:41545822-41545841 None:intergenic 45.0%
! ACTGGTGGATCCAAAGGAAT+AGG - chr4.4:41544167-41544186 MS.gene021164:CDS 45.0%
! CTGGTGGATCCAAAGGAATA+GGG - chr4.4:41544168-41544187 MS.gene021164:CDS 45.0%
!! TGAGTAATGGATGTGCAAGC+TGG + chr4.4:41545075-41545094 None:intergenic 45.0%
AAGAGGCTGCAGGTAAGCAT+AGG + chr4.4:41545874-41545893 None:intergenic 50.0%
ACCAAGGAAGACACCTCTTC+TGG + chr4.4:41545845-41545864 None:intergenic 50.0%
AGAACCCCTCTTGGACGTAT+TGG - chr4.4:41545815-41545834 MS.gene021164:intron 50.0%
CCACTGTACACACTTGTGCT+AGG - chr4.4:41544677-41544696 MS.gene021164:CDS 50.0%
CCTAGCACAAGTGTGTACAG+TGG + chr4.4:41544680-41544699 None:intergenic 50.0%
CCTGCAGCCTCTTTCATAAC+AGG - chr4.4:41545881-41545900 MS.gene021164:CDS 50.0%
CCTGTTATGAAAGAGGCTGC+AGG + chr4.4:41545884-41545903 None:intergenic 50.0%
GGAGCAATTAGCAGAGCTAG+GGG - chr4.4:41544654-41544673 MS.gene021164:intron 50.0%
GGTTCTCCAATACGTCCAAG+AGG + chr4.4:41545824-41545843 None:intergenic 50.0%
GTCATCTCGTGCACAAAGAG+AGG - chr4.4:41544777-41544796 MS.gene021164:intron 50.0%
GTGGAGCAATTAGCAGAGCT+AGG - chr4.4:41544652-41544671 MS.gene021164:intron 50.0%
TGATGCTACCCCAACAATGG+AGG + chr4.4:41545131-41545150 None:intergenic 50.0%
TTTGAAGGGGATGACAGCTC+TGG - chr4.4:41544142-41544161 MS.gene021164:CDS 50.0%
!! ACCAGAAGAGGTGTCTTCCT+TGG - chr4.4:41545841-41545860 MS.gene021164:intron 50.0%
!! AGAAGAGGTGTCTTCCTTGG+TGG - chr4.4:41545844-41545863 MS.gene021164:intron 50.0%
! ATGACAGCTCTGGTCACTGG+TGG - chr4.4:41544152-41544171 MS.gene021164:CDS 55.0%
! CTGGTCACTGGTGGATCCAA+AGG - chr4.4:41544161-41544180 MS.gene021164:CDS 55.0%
! GCAGCAAGAGGGGTTCTGAT+TGG + chr4.4:41545372-41545391 None:intergenic 55.0%
! AGAGGGGTTCTGATTGGCCC+TGG + chr4.4:41545366-41545385 None:intergenic 60.0%
! GGGATGACAGCTCTGGTCAC+TGG - chr4.4:41544149-41544168 MS.gene021164:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41544101 41545958 41544101 ID=MS.gene021164
chr4.4 mRNA 41544101 41545958 41544101 ID=MS.gene021164.t1;Parent=MS.gene021164
chr4.4 exon 41545870 41545958 41545870 ID=MS.gene021164.t1.exon1;Parent=MS.gene021164.t1
chr4.4 CDS 41545870 41545958 41545870 ID=cds.MS.gene021164.t1;Parent=MS.gene021164.t1
chr4.4 exon 41545210 41545417 41545210 ID=MS.gene021164.t1.exon2;Parent=MS.gene021164.t1
chr4.4 CDS 41545210 41545417 41545210 ID=cds.MS.gene021164.t1;Parent=MS.gene021164.t1
chr4.4 exon 41544875 41545091 41544875 ID=MS.gene021164.t1.exon3;Parent=MS.gene021164.t1
chr4.4 CDS 41544875 41545091 41544875 ID=cds.MS.gene021164.t1;Parent=MS.gene021164.t1
chr4.4 exon 41544664 41544770 41544664 ID=MS.gene021164.t1.exon4;Parent=MS.gene021164.t1
chr4.4 CDS 41544664 41544770 41544664 ID=cds.MS.gene021164.t1;Parent=MS.gene021164.t1
chr4.4 exon 41544101 41544283 41544101 ID=MS.gene021164.t1.exon5;Parent=MS.gene021164.t1
chr4.4 CDS 41544101 41544283 41544101 ID=cds.MS.gene021164.t1;Parent=MS.gene021164.t1
Gene Sequence

>MS.gene021164

ATGGCAGAGGGAAACACAAGTAGCAGAAATAGTAAGTGGTCTTTGAAGGGGATGACAGCTCTGGTCACTGGTGGATCCAAAGGAATAGGGTTGCTATCTTTTGTTCTTTTCCTCTTCTCTTATTCTTTTGTGCAGATTATTTTGCTTATTTCTCTTGTTGCAAATTAATTGATCTTTTAGCAGCTCTACCATAGATCATGAAATTAGTTGAGTTAGTCACAAATATACTATTTTAATTTTTAGCTAGCTACCCAAATTTTGTAGTTAATTGATCATAACTAATACTTTTTTTTAAACTGTAACTAATCCTAAATTAACTCAAAGCTAATTTCCTAGTTGTCAATAGTCTAATTCACCTAGTCAATAGTCATGATGCTTGTTTGGAAAAAAATTGTGCAAGCAAGTCAAACATGTTTATATTGACTAATAATAACCAATGGAACAATATTTTAACAAATTAAATATGATAGTCTTATATAAAAAATATTTATGAATCATGATTTTTCTCACTGTTTATATATGTGAATTAAACTATGACTAGGTATGATATAGTGGAGCAATTAGCAGAGCTAGGGGCCACTGTACACACTTGTGCTAGGAATGAAGCTAAACTCAATGAATGCTTACATCAATGGGTCACAAAAGGATACAAAATCACTGGTTCAGTCTGTGATGTGTCATCTCGTGCACAAAGAGAGGAGCTCATAGCTAGAGTCTCTTCTGAGTTCAATGGCAAACTCAATATCCTTGTAAGCAATTAATAATACAATACATGAAATTGAAGTTACTATTTTTTTCACCTTTTTATAGGATTTATATAGTTTGTTACATTAGACCGACATTTATTTATTTATGTATGAAATTTAGGTGAACAATGTGGGAACCAACATACCAAAACAATCACTAGATTTTACTGAGCAAGATTTCTCATTTCTGGTGAATACAAATCTTGAATCTGCTTTTCACATAAGCCAGCTTGCACATCCATTACTCAAAGCTTCAAACTCTGCAAGCATTGTTTTCATGTCCTCCATTGTTGGGGTAGCATCACTAAATGTAGGAACCATCTATAGTGCAGCAAAAGGTAACTGTTTCATGGTTTTAATTTAGTAAAATTGACACTGTAATTTTTTTTTTTTTGAACTAAAAATGTTTAATTTTAAAATATATATGTAATTTACAATGCAGGAGCAATAAACCAGCTTACAAAAAATTTGGCATGTGAATGGGCTAGAGACAACATAAGGACTAATTGTGTTGCACCAGGGCCAATCAGAACCCCTCTTGCTGCAGAAGTAATTTTCTATACTTGTTTGAGTATAATGATTTTACACTTTATCTAAAAAAATGTTTTACTCACAATTATCTAGTTACATCACGCCATGTACATATTTGATGAGAAAATAATATCATATGATGATATATAATTGTTAAAATTTGAGTTGCGCTTAACTCAACTCTATAAAACTAGTGTGTTCGATGTGGGATGATTAGCCCAACTCATTAGTCAGATACCAAAAAATTTAGATTGTGTCCAATCCATCCAACTCCTAGAAAATTGGCTAATACTGCGTGACTTGTCTCCCATTTATAAACTCGTTTTTAGGCCATATTTCATTCAATAAATATGTAAAAAAGAGTTACATGTTAATGCATAGTTATTTCCTTCTTGCAGATCTTAAAAGATGAAAAACTTCTCGATGCTTTCATTTCAAGAACCCCTCTTGGACGTATTGGAGAACCAGAAGAGGTGTCTTCCTTGGTGGCATTCCTATGCTTACCTGCAGCCTCTTTCATAACAGGACAGACCATTTGTATTGATGGCGGGTTAACTGTCAACTGTCTCCTTCTACAATAA
Protein sequence

>MS.gene021164.t1

MAEGNTSSRNSKWSLKGMTALVTGGSKGIGYDIVEQLAELGATVHTCARNEAKLNECLHQWVTKGYKITGSVCDVSSRAQREELIARVSSEFNGKLNILVNNVGTNIPKQSLDFTEQDFSFLVNTNLESAFHISQLAHPLLKASNSASIVFMSSIVGVASLNVGTIYSAAKGAINQLTKNLACEWARDNIRTNCVAPGPIRTPLAAEILKDEKLLDAFISRTPLGRIGEPEEVSSLVAFLCLPAASFITGQTICIDGGLTVNCLLLQ