AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045727.t1 MTR_2g064405 98.219 393 5 2 1 391 1 393 0.0 789
MS.gene045727.t1 MTR_2g064405 99.684 316 1 0 76 391 1 316 0.0 654
MS.gene045727.t1 MTR_8g046550 78.372 393 76 3 2 391 8 394 0.0 613
MS.gene045727.t1 MTR_3g080090 65.823 395 122 2 2 388 7 396 0.0 522
MS.gene045727.t1 MTR_7g022890 62.299 374 134 4 17 387 26 395 3.75e-160 456
MS.gene045727.t1 MTR_7g022890 65.569 334 113 2 55 387 10 342 3.83e-156 444
MS.gene045727.t1 MTR_3g062610 56.707 328 135 4 61 387 72 393 4.26e-127 372
MS.gene045727.t1 MTR_5g075680 53.939 330 145 4 61 389 12 335 3.94e-120 352
MS.gene045727.t1 MTR_5g075680 52.890 346 151 5 44 388 53 387 6.99e-120 353
MS.gene045727.t1 MTR_3g062610 52.564 234 105 3 61 293 72 300 1.19e-79 248
MS.gene045727.t1 MTR_5g075680 46.067 267 132 5 44 309 53 308 2.98e-71 226
MS.gene045727.t1 MTR_5g075680 42.289 201 105 4 44 243 53 243 1.20e-42 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045727.t1 AT1G16310 75.603 373 89 1 18 390 58 428 0.0 576
MS.gene045727.t1 AT1G79520 71.654 381 103 2 14 391 24 402 0.0 543
MS.gene045727.t1 AT1G79520 69.466 393 103 3 14 391 24 414 0.0 535
MS.gene045727.t1 AT1G79520 76.471 340 80 0 52 391 10 349 0.0 534
MS.gene045727.t1 AT1G79520 76.471 340 80 0 52 391 10 349 0.0 534
MS.gene045727.t1 AT1G16310 79.048 315 66 0 76 390 1 315 0.0 509
MS.gene045727.t1 AT2G39450 59.733 375 146 3 17 390 22 392 3.27e-139 402
MS.gene045727.t1 AT3G58060 54.381 331 147 3 61 387 71 401 8.95e-122 358
MS.gene045727.t1 AT3G58060 54.381 331 147 3 61 387 71 401 8.95e-122 358

Find 69 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTTAACATACCTTATA+TGG 0.187659 2.3:+30510047 MS.gene045727:CDS
ATCATCCAATTCACCTTTAC+TGG 0.222158 2.3:-30509058 None:intergenic
GTTCATGGTGGTTCCATATA+AGG 0.230476 2.3:-30510060 None:intergenic
ACAAAAGCTCTTTCAACTTC+TGG 0.237828 2.3:-30510226 None:intergenic
GTTAACTTTGCCAAGAATTC+AGG 0.263487 2.3:-30510034 None:intergenic
TTCTAGCTGTCAAGTTCTTC+TGG 0.275479 2.3:+30509221 MS.gene045727:CDS
AACAGCACCACCTGAATTCT+TGG 0.286135 2.3:+30510024 MS.gene045727:CDS
ATGAAGTTTCAGACGGTTCT+TGG 0.288603 2.3:+30506355 MS.gene045727:CDS
CTATGTGCTTGATTTGTTCA+TGG 0.301014 2.3:-30510075 None:intergenic
GAATTGGATGATCGGGATTA+TGG 0.321624 2.3:+30509069 MS.gene045727:CDS
TTCCAATCGGATAGTGATAT+CGG 0.334945 2.3:-30508680 None:intergenic
ACCTTTACTGGATCGACTTC+TGG 0.344775 2.3:-30509046 None:intergenic
CTCTTGTTTCTTGTAATACT+CGG 0.363210 2.3:-30507990 None:intergenic
ATCAACACTAGACTCTCTTT+TGG 0.380655 2.3:+30508602 MS.gene045727:CDS
TCGATCCAGTAAAGGTGAAT+TGG 0.389302 2.3:+30509053 MS.gene045727:CDS
TGCATCCGTGATGGCAACCT+TGG 0.401323 2.3:+30508855 MS.gene045727:CDS
CTTGGTGTTGTTTGCAGCAA+AGG 0.416822 2.3:+30508539 MS.gene045727:CDS
TCTTGTCTGGTTTCATATTG+TGG 0.420819 2.3:+30508628 MS.gene045727:CDS
CCTCGAAGGATTTAATGAAA+TGG 0.427590 2.3:+30508018 MS.gene045727:CDS
ATCACCTCAGAGAGACCTCC+TGG 0.432671 2.3:-30508067 None:intergenic
AAATCTAAGTTCAGTTTCAA+TGG 0.438769 2.3:+30506435 MS.gene045727:CDS
GTTTGGCTTGCTCATGGCAT+TGG 0.439654 2.3:-30508658 None:intergenic
CCATTTCATTAAATCCTTCG+AGG 0.445178 2.3:-30508018 None:intergenic
GCATCCGTGATGGCAACCTT+GGG 0.449922 2.3:+30508856 MS.gene045727:CDS
TGGTTGAGAAACATGTCTTC+TGG 0.452682 2.3:-30510157 None:intergenic
ATGGAAGCTATGCATGAAAC+TGG 0.456771 2.3:+30508037 MS.gene045727:CDS
TTTCGCCGATGTTGTGTGCT+TGG 0.461751 2.3:-30510177 None:intergenic
GAGAATGTCTGGTCACTCAT+TGG 0.462981 2.3:+30509998 MS.gene045727:CDS
ATCGGATAGTGATATCGGTT+TGG 0.466363 2.3:-30508675 None:intergenic
AGACAGTGATGGAGAATGTC+TGG 0.467975 2.3:+30509987 MS.gene045727:CDS
TAAAGGTGAATTGGATGATC+GGG 0.471055 2.3:+30509062 MS.gene045727:CDS
TGGAAAGAAACGCATGCAAC+CGG 0.477866 2.3:+30508698 MS.gene045727:CDS
TGCATGCGTTTCTTTCCAAT+CGG 0.484472 2.3:-30508693 None:intergenic
ACAGTTAGAGCATACACGTT+TGG 0.487330 2.3:+30510100 MS.gene045727:CDS
TTCTGGTGGATGGATCCAAC+AGG 0.504950 2.3:+30509238 MS.gene045727:CDS
CGAAACACTTCAAGAGAAGC+TGG 0.507714 2.3:+30510195 MS.gene045727:CDS
AACCGATATCACTATCCGAT+TGG 0.515935 2.3:+30508678 MS.gene045727:CDS
CATACTGATGTCTTATGCTC+AGG 0.516704 2.3:-30510277 None:intergenic
ATATCGGTTTGGCTTGCTCA+TGG 0.516883 2.3:-30508664 None:intergenic
CAAGCCCAAGGTTGCCATCA+CGG 0.531642 2.3:-30508860 None:intergenic
GTTTGGTGCAGATTACTTTG+TGG 0.541154 2.3:+30510117 MS.gene045727:CDS
TGTCAAGTTCTTCTGGTGGA+TGG 0.542414 2.3:+30509228 MS.gene045727:CDS
CTTCTTGATGAAGTTTCAGA+CGG 0.545581 2.3:+30506348 MS.gene045727:CDS
TAATGATCTGCTCTCGATTG+AGG 0.549611 2.3:-30508568 None:intergenic
CTTACAAGAATAGCTCCTGT+TGG 0.551242 2.3:-30509253 None:intergenic
GCCAGAAGTCGATCCAGTAA+AGG 0.564036 2.3:+30509045 MS.gene045727:CDS
TAGCTGTCAAGTTCTTCTGG+TGG 0.564250 2.3:+30509224 MS.gene045727:CDS
AACTTTGCCAAGAATTCAGG+TGG 0.573238 2.3:-30510031 None:intergenic
GTAAAGGTGAATTGGATGAT+CGG 0.574495 2.3:+30509061 MS.gene045727:CDS
CACTATATACAATCAACACA+TGG 0.581728 2.3:+30509960 MS.gene045727:CDS
ACTTGCAAAGAGTGAGAGGA+CGG 0.587244 2.3:+30508491 MS.gene045727:CDS
TGTGTCAAATGCATGTAACT+TGG 0.588818 2.3:+30508521 MS.gene045727:CDS
GATGTCATTACTAATTCCGT+CGG 0.610281 2.3:+30509184 MS.gene045727:CDS
TGATCAACAACATTCTGTGA+TGG 0.612919 2.3:-30506405 None:intergenic
AGCAACTTGCAAAGAGTGAG+AGG 0.619088 2.3:+30508487 MS.gene045727:CDS
GATCAAAATCTGCAAGCCCA+AGG 0.622295 2.3:-30508872 None:intergenic
AACAGCGGCGGCTAATCCGA+CGG 0.625123 2.3:-30509200 None:intergenic
TCTGAAACTTCATCAAGAAG+AGG 0.625658 2.3:-30506345 None:intergenic
GAACTTGACAGCTAGAACAG+CGG 0.628290 2.3:-30509215 None:intergenic
AAACAAGAGAGACTCCTCGA+AGG 0.629592 2.3:+30508004 MS.gene045727:CDS
CTCAACCAAGCACACAACAT+CGG 0.630772 2.3:+30510172 MS.gene045727:CDS
TCACCTCAGAGAGACCTCCT+GGG 0.631079 2.3:-30508066 None:intergenic
TGTGCTTGATTTGTTCATGG+TGG 0.640773 2.3:-30510072 None:intergenic
TTTCCCAGGAGGTCTCTCTG+AGG 0.647360 2.3:+30508063 MS.gene045727:CDS
CACATGGACAAAGACAGTGA+TGG 0.674860 2.3:+30509976 MS.gene045727:CDS
AAAGAAACGCATGCAACCGG+TGG 0.685895 2.3:+30508701 MS.gene045727:CDS
TGTTTCAGGGAAACAACGCA+AGG 0.686235 2.3:+30507964 MS.gene045727:intron
TTCAGGGAAACAACGCAAGG+TGG 0.700523 2.3:+30507967 MS.gene045727:intron
CTTGACAGCTAGAACAGCGG+CGG 0.725540 2.3:-30509212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATATTATAAATT+AGG - chr2.3:30507307-30507326 None:intergenic 0.0%
!!! AATTTATAATATTTTTTTAA+AGG + chr2.3:30507307-30507326 MS.gene045727:intron 0.0%
!! ATAGTTTATGTTTAATATCA+AGG - chr2.3:30506880-30506899 None:intergenic 15.0%
!!! ACTTACAATTTTCTTATAAA+CGG + chr2.3:30508112-30508131 MS.gene045727:intron 15.0%
!!! ACTTTTAAATTCTCTATTTT+TGG + chr2.3:30506934-30506953 MS.gene045727:intron 15.0%
!!! TTATTATCAATTTTTTGTTC+AGG + chr2.3:30507711-30507730 MS.gene045727:intron 15.0%
!!! TTCTAAAGTTTATGTTTTTT+TGG + chr2.3:30506497-30506516 MS.gene045727:intron 15.0%
!! AAACAGAAAAGGAATAAAAT+AGG - chr2.3:30506698-30506717 None:intergenic 20.0%
!! GCAACAACAAAATAAAATAA+TGG - chr2.3:30507197-30507216 None:intergenic 20.0%
!! TAATGGAAAAATACTAATTG+TGG - chr2.3:30509341-30509360 None:intergenic 20.0%
!! TAGAAAACTTTAAACAGAAA+AGG - chr2.3:30506709-30506728 None:intergenic 20.0%
!! TTAAACAAACCGTTATTATT+CGG - chr2.3:30508309-30508328 None:intergenic 20.0%
!! TTAACTTTATTATGTGAACA+AGG - chr2.3:30507383-30507402 None:intergenic 20.0%
!! TTTATGTTTAATATCAAGGT+GGG - chr2.3:30506876-30506895 None:intergenic 20.0%
!!! TATCATGATCTTACTTTTTA+AGG - chr2.3:30506984-30507003 None:intergenic 20.0%
!!! TATGAAAATTCTGAAATTTC+TGG + chr2.3:30507437-30507456 MS.gene045727:intron 20.0%
!!! TGCTTTATTGTAATAATAGT+GGG + chr2.3:30507021-30507040 MS.gene045727:intron 20.0%
!!! TTATTTTTCAACTTGCTTTT+AGG + chr2.3:30507761-30507780 MS.gene045727:intron 20.0%
!!! TTGCTTTATTGTAATAATAG+TGG + chr2.3:30507020-30507039 MS.gene045727:intron 20.0%
!!! TTTAAATTCTCTATTTTTGG+TGG + chr2.3:30506937-30506956 MS.gene045727:intron 20.0%
!!! TTTAATTAGTTCAACTGTTT+TGG + chr2.3:30509655-30509674 MS.gene045727:intron 20.0%
! AAAAATGGTCGAAAAATTCT+AGG - chr2.3:30509721-30509740 None:intergenic 25.0%
! AAAAGCAAAGAACAACATAA+TGG - chr2.3:30506546-30506565 None:intergenic 25.0%
! AAAATAACTGACCATAGTAA+AGG + chr2.3:30509621-30509640 MS.gene045727:intron 25.0%
! AAAATTGCATTATGTTTGTC+AGG + chr2.3:30508455-30508474 MS.gene045727:intron 25.0%
! AAAGCAAAGAACAACATAAT+GGG - chr2.3:30506545-30506564 None:intergenic 25.0%
! AAATCTAAGTTCAGTTTCAA+TGG + chr2.3:30506435-30506454 MS.gene045727:CDS 25.0%
! AAATTTAAGAATGACTCACT+AGG - chr2.3:30506474-30506493 None:intergenic 25.0%
! AATCACCAATGTTATCAAAT+AGG - chr2.3:30508360-30508379 None:intergenic 25.0%
! ACACTTTGTAGTAAAAATCT+CGG - chr2.3:30506811-30506830 None:intergenic 25.0%
! AGGAATAAAATAGGTGAAAA+AGG - chr2.3:30506689-30506708 None:intergenic 25.0%
! AGTTACGGGAAATTTATTAA+TGG - chr2.3:30509358-30509377 None:intergenic 25.0%
! ATCACCAATGTTATCAAATA+GGG - chr2.3:30508359-30508378 None:intergenic 25.0%
! CAAAGTTAACATACCTTATA+TGG + chr2.3:30510047-30510066 MS.gene045727:CDS 25.0%
! GAACTAATTAAACCTTAAAC+AGG - chr2.3:30509647-30509666 None:intergenic 25.0%
! GACAAAATAATGATGTGTAA+AGG - chr2.3:30507279-30507298 None:intergenic 25.0%
! GTTTATGTTTAATATCAAGG+TGG - chr2.3:30506877-30506896 None:intergenic 25.0%
! TCAAAAGCTACAAATTCTAT+TGG - chr2.3:30507676-30507695 None:intergenic 25.0%
! TGATTACCTTTGCAAAAATT+TGG - chr2.3:30508909-30508928 None:intergenic 25.0%
! TTATTATGTGAACAAGGTAA+TGG - chr2.3:30507377-30507396 None:intergenic 25.0%
! TTGATGAATATCCATCATAA+AGG - chr2.3:30507068-30507087 None:intergenic 25.0%
!! AAAACTGATTTAAAGATGCT+TGG + chr2.3:30507610-30507629 MS.gene045727:intron 25.0%
!! AAAGTTTCAATTTTGTGTGA+TGG + chr2.3:30506593-30506612 MS.gene045727:intron 25.0%
!! AAGTTTCAATTTTGTGTGAT+GGG + chr2.3:30506594-30506613 MS.gene045727:intron 25.0%
!! AGTATCATTCTTTTTCTTAC+AGG + chr2.3:30509018-30509037 MS.gene045727:intron 25.0%
!! GCTTTATTGTAATAATAGTG+GGG + chr2.3:30507022-30507041 MS.gene045727:intron 25.0%
!! TCTATTGGAATATTTTCTAG+TGG - chr2.3:30507661-30507680 None:intergenic 25.0%
!! TTTACTATGGTCAGTTATTT+TGG - chr2.3:30509622-30509641 None:intergenic 25.0%
!! TTTGTAGCTTTTGACAATTA+AGG + chr2.3:30507682-30507701 MS.gene045727:intron 25.0%
!!! AGAATTTTTCGACCATTTTT+CGG + chr2.3:30509721-30509740 MS.gene045727:intron 25.0%
!!! ATTTTTAAAACACTGCTTCT+AGG - chr2.3:30509509-30509528 None:intergenic 25.0%
!!! CTTTTTTGTCATATGATTGT+TGG + chr2.3:30509866-30509885 MS.gene045727:intron 25.0%
ACACGATTTAGTATAAAGTG+TGG - chr2.3:30508282-30508301 None:intergenic 30.0%
AGGTGAAAAAGGATTAACAA+TGG - chr2.3:30506678-30506697 None:intergenic 30.0%
CACTATATACAATCAACACA+TGG + chr2.3:30509960-30509979 MS.gene045727:CDS 30.0%
CATGACTTAAAGTGTATGTT+TGG + chr2.3:30507550-30507569 MS.gene045727:intron 30.0%
GTGTATGTTTGATATTATCC+TGG + chr2.3:30506761-30506780 MS.gene045727:intron 30.0%
TAAATGAAGAGCCTTTATGA+TGG + chr2.3:30507054-30507073 MS.gene045727:intron 30.0%
TACATGTGTTTGAATCGATT+AGG - chr2.3:30508393-30508412 None:intergenic 30.0%
TATAAAGTGTGGTCAAATAG+TGG - chr2.3:30508271-30508290 None:intergenic 30.0%
TCACCAATGTTATCAAATAG+GGG - chr2.3:30508358-30508377 None:intergenic 30.0%
TGTGTAAAGGAATTAAGTGA+AGG - chr2.3:30507266-30507285 None:intergenic 30.0%
TTGAATCGATTAGGTTCAAT+TGG - chr2.3:30508384-30508403 None:intergenic 30.0%
! AAAGTGTATGTTTGGTTTCA+TGG + chr2.3:30507558-30507577 MS.gene045727:intron 30.0%
! AAATACTTATGTTTCTGTGC+AGG + chr2.3:30508819-30508838 MS.gene045727:intron 30.0%
! AAGCTTTGATTGTGTACAAA+TGG - chr2.3:30507226-30507245 None:intergenic 30.0%
! AATACTTATGTTTCTGTGCA+GGG + chr2.3:30508820-30508839 MS.gene045727:intron 30.0%
! CTCTTGTTTCTTGTAATACT+CGG - chr2.3:30507993-30508012 None:intergenic 30.0%
! GAATGTGAAGTGTTTATGTT+TGG - chr2.3:30507803-30507822 None:intergenic 30.0%
!! CTAGTATGTGTTTGATTTTG+CGG - chr2.3:30507872-30507891 None:intergenic 30.0%
!!! CAATTTTTTGTTCAGGTGAA+GGG + chr2.3:30507718-30507737 MS.gene045727:intron 30.0%
!!! TAATTTTTTACCAGCTTCTC+AGG - chr2.3:30507474-30507493 None:intergenic 30.0%
!!! TCAATTTTTTGTTCAGGTGA+AGG + chr2.3:30507717-30507736 MS.gene045727:intron 30.0%
!!! TTAGTTCAACTGTTTTGGTT+TGG + chr2.3:30509660-30509679 MS.gene045727:intron 30.0%
AACTCAATGTTTGTATAGCC+CGG + chr2.3:30509820-30509839 MS.gene045727:intron 35.0%
ACAAAAGCTCTTTCAACTTC+TGG - chr2.3:30510229-30510248 None:intergenic 35.0%
ACATCAAAATGTTGTCGAAG+GGG + chr2.3:30509411-30509430 MS.gene045727:intron 35.0%
AGTAATCCCCAAAGTTACTT+CGG + chr2.3:30509777-30509796 MS.gene045727:intron 35.0%
ATCACACAATCATCATTAGC+AGG - chr2.3:30509918-30509937 None:intergenic 35.0%
ATCATCCAATTCACCTTTAC+TGG - chr2.3:30509061-30509080 None:intergenic 35.0%
CACATCAAAATGTTGTCGAA+GGG + chr2.3:30509410-30509429 MS.gene045727:intron 35.0%
CCATTTCATTAAATCCTTCG+AGG - chr2.3:30508021-30508040 None:intergenic 35.0%
CCTCGAAGGATTTAATGAAA+TGG + chr2.3:30508018-30508037 MS.gene045727:CDS 35.0%
CGTGTATTGCCGAATAATAA+CGG + chr2.3:30508297-30508316 MS.gene045727:intron 35.0%
CTTCTTGATGAAGTTTCAGA+CGG + chr2.3:30506348-30506367 MS.gene045727:CDS 35.0%
GATGTCATTACTAATTCCGT+CGG + chr2.3:30509184-30509203 MS.gene045727:CDS 35.0%
GTAAAGGTGAATTGGATGAT+CGG + chr2.3:30509061-30509080 MS.gene045727:CDS 35.0%
GTTAACTTTGCCAAGAATTC+AGG - chr2.3:30510037-30510056 None:intergenic 35.0%
TAAAGGTGAATTGGATGATC+GGG + chr2.3:30509062-30509081 MS.gene045727:CDS 35.0%
TATCAATTTACCTGAGAAGC+TGG + chr2.3:30507461-30507480 MS.gene045727:intron 35.0%
TGATCAACAACATTCTGTGA+TGG - chr2.3:30506408-30506427 None:intergenic 35.0%
TGTACTTCCGAAGTAACTTT+GGG - chr2.3:30509787-30509806 None:intergenic 35.0%
TGTGTCAAATGCATGTAACT+TGG + chr2.3:30508521-30508540 MS.gene045727:CDS 35.0%
TTCCAATCGGATAGTGATAT+CGG - chr2.3:30508683-30508702 None:intergenic 35.0%
TTTGATGTGGCTTATACGAA+AGG - chr2.3:30509399-30509418 None:intergenic 35.0%
! ATCAACACTAGACTCTCTTT+TGG + chr2.3:30508602-30508621 MS.gene045727:CDS 35.0%
! ATGATTGTTTGCTGGTTAGA+GGG + chr2.3:30509446-30509465 MS.gene045727:intron 35.0%
! ATTTTCTTCGCATTTCAGTG+TGG + chr2.3:30509292-30509311 MS.gene045727:intron 35.0%
! TCTGAAACTTCATCAAGAAG+AGG - chr2.3:30506348-30506367 None:intergenic 35.0%
! TCTTGTCTGGTTTCATATTG+TGG + chr2.3:30508628-30508647 MS.gene045727:CDS 35.0%
!! ATCGTGATTTTGACATATCC+AGG - chr2.3:30506782-30506801 None:intergenic 35.0%
!! CAAAGAACAACATAATGGGT+CGG - chr2.3:30506541-30506560 None:intergenic 35.0%
!! CTATGTGCTTGATTTGTTCA+TGG - chr2.3:30510078-30510097 None:intergenic 35.0%
!!! GATTTTTAGCGTGTCAATTG+CGG + chr2.3:30508183-30508202 MS.gene045727:intron 35.0%
AAAGGAGTTTACACCCATTG+TGG - chr2.3:30509381-30509400 None:intergenic 40.0%
AACCGATATCACTATCCGAT+TGG + chr2.3:30508678-30508697 MS.gene045727:CDS 40.0%
AACTTTGCCAAGAATTCAGG+TGG - chr2.3:30510034-30510053 None:intergenic 40.0%
AATCTAAGTTGTCCTACCAC+CGG - chr2.3:30508720-30508739 None:intergenic 40.0%
AATTGAACCGAACATCGAAC+TGG + chr2.3:30509526-30509545 MS.gene045727:intron 40.0%
AATTTCCCGTAACTCCACAA+TGG + chr2.3:30509364-30509383 MS.gene045727:intron 40.0%
ACAGTTAGAGCATACACGTT+TGG + chr2.3:30510100-30510119 MS.gene045727:CDS 40.0%
ATGAAGTTTCAGACGGTTCT+TGG + chr2.3:30506355-30506374 MS.gene045727:CDS 40.0%
ATGGAAGCTATGCATGAAAC+TGG + chr2.3:30508037-30508056 MS.gene045727:CDS 40.0%
ATTTCCCGTAACTCCACAAT+GGG + chr2.3:30509365-30509384 MS.gene045727:intron 40.0%
CATACTGATGTCTTATGCTC+AGG - chr2.3:30510280-30510299 None:intergenic 40.0%
CATCAAAATGTTGTCGAAGG+GGG + chr2.3:30509412-30509431 MS.gene045727:intron 40.0%
CCACATCAAAATGTTGTCGA+AGG + chr2.3:30509409-30509428 MS.gene045727:intron 40.0%
CCATAGTAAAGGCCTGTTTA+AGG + chr2.3:30509632-30509651 MS.gene045727:intron 40.0%
CCTTAAACAGGCCTTTACTA+TGG - chr2.3:30509635-30509654 None:intergenic 40.0%
CTTACAAGAATAGCTCCTGT+TGG - chr2.3:30509256-30509275 None:intergenic 40.0%
CTTTGCAAAAATTTGGCGAC+CGG - chr2.3:30508902-30508921 None:intergenic 40.0%
GAAACAGAGAAAGTAGGCAA+AGG - chr2.3:30507950-30507969 None:intergenic 40.0%
GAATTGGATGATCGGGATTA+TGG + chr2.3:30509069-30509088 MS.gene045727:CDS 40.0%
GATGCTTGGATGATGTTGAT+TGG + chr2.3:30507624-30507643 MS.gene045727:intron 40.0%
GCAATCTGCAATGAACATTC+AGG - chr2.3:30509941-30509960 None:intergenic 40.0%
GTACTTCCGAAGTAACTTTG+GGG - chr2.3:30509786-30509805 None:intergenic 40.0%
GTGTACTTCCGAAGTAACTT+TGG - chr2.3:30509788-30509807 None:intergenic 40.0%
GTTCATGGTGGTTCCATATA+AGG - chr2.3:30510063-30510082 None:intergenic 40.0%
TAATGATCTGCTCTCGATTG+AGG - chr2.3:30508571-30508590 None:intergenic 40.0%
TCGATCCAGTAAAGGTGAAT+TGG + chr2.3:30509053-30509072 MS.gene045727:CDS 40.0%
TGCATGAAGCTTCCGAAAAA+TGG - chr2.3:30509736-30509755 None:intergenic 40.0%
TGCATGCGTTTCTTTCCAAT+CGG - chr2.3:30508696-30508715 None:intergenic 40.0%
TGCCTACTTTCTCTGTTTCA+GGG + chr2.3:30507951-30507970 MS.gene045727:intron 40.0%
TGGTTGAGAAACATGTCTTC+TGG - chr2.3:30510160-30510179 None:intergenic 40.0%
TGTGTCACATGATTGTTTGC+TGG + chr2.3:30509438-30509457 MS.gene045727:intron 40.0%
TTACACCCATTGTGGAGTTA+CGG - chr2.3:30509373-30509392 None:intergenic 40.0%
TTCCCTGAAACAGAGAAAGT+AGG - chr2.3:30507956-30507975 None:intergenic 40.0%
TTGCCTACTTTCTCTGTTTC+AGG + chr2.3:30507950-30507969 MS.gene045727:intron 40.0%
! AGTAACTTTGGGGATTACTC+CGG - chr2.3:30509776-30509795 None:intergenic 40.0%
! ATCGGATAGTGATATCGGTT+TGG - chr2.3:30508678-30508697 None:intergenic 40.0%
! CATGATTGTTTGCTGGTTAG+AGG + chr2.3:30509445-30509464 MS.gene045727:intron 40.0%
! GCACCCCTATTTGATAACAT+TGG + chr2.3:30508352-30508371 MS.gene045727:intron 40.0%
! TCTCTTTTGGATCTCTTGTC+TGG + chr2.3:30508615-30508634 MS.gene045727:CDS 40.0%
! TTCTAGCTGTCAAGTTCTTC+TGG + chr2.3:30509221-30509240 MS.gene045727:CDS 40.0%
! TTGCGGATTGCGGAAAATAT+CGG + chr2.3:30508200-30508219 MS.gene045727:intron 40.0%
!! CCTTCGACAACATTTTGATG+TGG - chr2.3:30509412-30509431 None:intergenic 40.0%
!! GTTTGGTGCAGATTACTTTG+TGG + chr2.3:30510117-30510136 MS.gene045727:CDS 40.0%
!! TGTGCTTGATTTGTTCATGG+TGG - chr2.3:30510075-30510094 None:intergenic 40.0%
!! TTGCAGATTTTGATCGAGTC+CGG + chr2.3:30508880-30508899 MS.gene045727:CDS 40.0%
!!! CATGAAACTGGTTTTTTCCC+AGG + chr2.3:30508049-30508068 MS.gene045727:CDS 40.0%
!!! CATTGTTTTTGCATCCGTGA+TGG + chr2.3:30508846-30508865 MS.gene045727:CDS 40.0%
!!! GTGTAGCTTTTCGAGTATCA+CGG + chr2.3:30506827-30506846 MS.gene045727:intron 40.0%
AAACAAGAGAGACTCCTCGA+AGG + chr2.3:30508004-30508023 MS.gene045727:CDS 45.0%
AACAGCACCACCTGAATTCT+TGG + chr2.3:30510024-30510043 MS.gene045727:CDS 45.0%
ACTTGCAAAGAGTGAGAGGA+CGG + chr2.3:30508491-30508510 MS.gene045727:CDS 45.0%
AGCAACTTGCAAAGAGTGAG+AGG + chr2.3:30508487-30508506 MS.gene045727:CDS 45.0%
AGGTGGGTATGTCAGAATCA+CGG - chr2.3:30506860-30506879 None:intergenic 45.0%
AGTTCGAGAGCAGTTAAAGG+CGG - chr2.3:30509576-30509595 None:intergenic 45.0%
CACATGGACAAAGACAGTGA+TGG + chr2.3:30509976-30509995 MS.gene045727:CDS 45.0%
CCTAGTTCGAGAGCAGTTAA+AGG - chr2.3:30509579-30509598 None:intergenic 45.0%
CCTTTAACTGCTCTCGAACT+AGG + chr2.3:30509576-30509595 MS.gene045727:intron 45.0%
CGAAACACTTCAAGAGAAGC+TGG + chr2.3:30510195-30510214 MS.gene045727:CDS 45.0%
CTCAACCAAGCACACAACAT+CGG + chr2.3:30510172-30510191 MS.gene045727:CDS 45.0%
GAACTTGACAGCTAGAACAG+CGG - chr2.3:30509218-30509237 None:intergenic 45.0%
GAGAATGTCTGGTCACTCAT+TGG + chr2.3:30509998-30510017 MS.gene045727:CDS 45.0%
GATCAAAATCTGCAAGCCCA+AGG - chr2.3:30508875-30508894 None:intergenic 45.0%
TACACCCATTGTGGAGTTAC+GGG - chr2.3:30509372-30509391 None:intergenic 45.0%
TAGCTGTCAAGTTCTTCTGG+TGG + chr2.3:30509224-30509243 MS.gene045727:CDS 45.0%
TGATGTCTGGTGCAACAGTA+AGG + chr2.3:30509469-30509488 MS.gene045727:intron 45.0%
TGGAAAGAAACGCATGCAAC+CGG + chr2.3:30508698-30508717 MS.gene045727:CDS 45.0%
TGTCAAGTTCTTCTGGTGGA+TGG + chr2.3:30509228-30509247 MS.gene045727:CDS 45.0%
TGTTTCAGGGAAACAACGCA+AGG + chr2.3:30507964-30507983 MS.gene045727:intron 45.0%
TTTATCCAGACGTACACGTC+CGG - chr2.3:30509842-30509861 None:intergenic 45.0%
! ACCTTTACTGGATCGACTTC+TGG - chr2.3:30509049-30509068 None:intergenic 45.0%
! AGACAGTGATGGAGAATGTC+TGG + chr2.3:30509987-30510006 MS.gene045727:CDS 45.0%
! CGGTCGCCAAATTTTTGCAA+AGG + chr2.3:30508900-30508919 MS.gene045727:CDS 45.0%
! GTAACTTTGGGGATTACTCC+GGG - chr2.3:30509775-30509794 None:intergenic 45.0%
!! ATATCGGTTTGGCTTGCTCA+TGG - chr2.3:30508667-30508686 None:intergenic 45.0%
!! CTTGGTGTTGTTTGCAGCAA+AGG + chr2.3:30508539-30508558 MS.gene045727:CDS 45.0%
!!! GAAACTGGTTTTTTCCCAGG+AGG + chr2.3:30508052-30508071 MS.gene045727:CDS 45.0%
AAAGAAACGCATGCAACCGG+TGG + chr2.3:30508701-30508720 MS.gene045727:CDS 50.0%
GATTACTCCGGGAAAAAGGG+CGG - chr2.3:30509764-30509783 None:intergenic 50.0%
GCCAGAAGTCGATCCAGTAA+AGG + chr2.3:30509045-30509064 MS.gene045727:CDS 50.0%
GCGGTTGAACCACGAATCAA+AGG - chr2.3:30509557-30509576 None:intergenic 50.0%
GGGGATTACTCCGGGAAAAA+GGG - chr2.3:30509767-30509786 None:intergenic 50.0%
TGGGGATTACTCCGGGAAAA+AGG - chr2.3:30509768-30509787 None:intergenic 50.0%
TTATCCAGACGTACACGTCC+GGG - chr2.3:30509841-30509860 None:intergenic 50.0%
TTCAGGGAAACAACGCAAGG+TGG + chr2.3:30507967-30507986 MS.gene045727:intron 50.0%
TTTCGCCGATGTTGTGTGCT+TGG - chr2.3:30510180-30510199 None:intergenic 50.0%
! AGCGTGTCAATTGCGGATTG+CGG + chr2.3:30508190-30508209 MS.gene045727:intron 50.0%
! CATTAAGCCGCCCTTTTTCC+CGG + chr2.3:30509754-30509773 MS.gene045727:intron 50.0%
! TTCTGGTGGATGGATCCAAC+AGG + chr2.3:30509238-30509257 MS.gene045727:CDS 50.0%
!! GTTTGGCTTGCTCATGGCAT+TGG - chr2.3:30508661-30508680 None:intergenic 50.0%
AAACGCATGCAACCGGTGGT+AGG + chr2.3:30508705-30508724 MS.gene045727:intron 55.0%
ATCACCTCAGAGAGACCTCC+TGG - chr2.3:30508070-30508089 None:intergenic 55.0%
CAAGCCCAAGGTTGCCATCA+CGG - chr2.3:30508863-30508882 None:intergenic 55.0%
CTTGACAGCTAGAACAGCGG+CGG - chr2.3:30509215-30509234 None:intergenic 55.0%
GCATCCGTGATGGCAACCTT+GGG + chr2.3:30508856-30508875 MS.gene045727:CDS 55.0%
GCTGGTTAGAGGGTGATGTC+TGG + chr2.3:30509456-30509475 MS.gene045727:intron 55.0%
GGTCACGCCAGTTCGATGTT+CGG - chr2.3:30509536-30509555 None:intergenic 55.0%
TCACCTCAGAGAGACCTCCT+GGG - chr2.3:30508069-30508088 None:intergenic 55.0%
TGCATCCGTGATGGCAACCT+TGG + chr2.3:30508855-30508874 MS.gene045727:CDS 55.0%
TTTCCCAGGAGGTCTCTCTG+AGG + chr2.3:30508063-30508082 MS.gene045727:CDS 55.0%
! CTGGCGTGACCTTTGATTCG+TGG + chr2.3:30509545-30509564 MS.gene045727:intron 55.0%
AACAGCGGCGGCTAATCCGA+CGG - chr2.3:30509203-30509222 None:intergenic 60.0%
ATAGCCCGGACGTGTACGTC+TGG + chr2.3:30509834-30509853 MS.gene045727:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 30506315 30510300 30506315 ID=MS.gene045727
chr2.3 mRNA 30506315 30510300 30506315 ID=MS.gene045727.t1;Parent=MS.gene045727
chr2.3 exon 30506315 30506474 30506315 ID=MS.gene045727.t1.exon1;Parent=MS.gene045727.t1
chr2.3 CDS 30506315 30506474 30506315 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
chr2.3 exon 30507972 30508084 30507972 ID=MS.gene045727.t1.exon2;Parent=MS.gene045727.t1
chr2.3 CDS 30507972 30508084 30507972 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
chr2.3 exon 30508477 30508722 30508477 ID=MS.gene045727.t1.exon3;Parent=MS.gene045727.t1
chr2.3 CDS 30508477 30508722 30508477 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
chr2.3 exon 30508841 30508921 30508841 ID=MS.gene045727.t1.exon4;Parent=MS.gene045727.t1
chr2.3 CDS 30508841 30508921 30508841 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
chr2.3 exon 30509040 30509270 30509040 ID=MS.gene045727.t1.exon5;Parent=MS.gene045727.t1
chr2.3 CDS 30509040 30509270 30509040 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
chr2.3 exon 30509956 30510300 30509956 ID=MS.gene045727.t1.exon6;Parent=MS.gene045727.t1
chr2.3 CDS 30509956 30510300 30509956 ID=cds.MS.gene045727.t1;Parent=MS.gene045727.t1
Gene Sequence

>MS.gene045727

ATGGCTGATAGTGACAGCATACGAAGTGAACCTCTTCTTGATGAAGTTTCAGACGGTTCTTGGAGACTTAATGTTAAAGAATTTCATCTACCATCACAGAATGTTGTTGATCATGAAAAGAAATCTAAGTTCAGTTTCAATGGTCTTCTTCGTAAGCCTAGTGAGTCATTCTTAAATTTATGTTCTAAAGTTTATGTTTTTTTGGTGTGTTGAATTGTGTGAACCGACCCATTATGTTGTTCTTTGCTTTTTTCAAGATTTTCTTTCATTGATTGATTAAAGTTTCAATTTTGTGTGATGGGTTTGTTCAGTTTTCTGTGATTATGTTGTGCATGTGTTGCTGTTGAATTCTGCAATAAGCCATTGTTAATCCTTTTTCACCTATTTTATTCCTTTTCTGTTTAAAGTTTTCTACTTTTGTGTAATGAGTATGTTTTGTTTTTCATGTGTATGTTTGATATTATCCTGGATATGTCAAAATCACGATGATTTACCGAGATTTTTACTACAAAGTGTAGCTTTTCGAGTATCACGGTGAGTCGCCGTGATTCTGACATACCCACCTTGATATTAAACATAAACTATGATTATGTTTATGTTGCGTATTGAATTCTGCAAAACTTTTAAATTCTCTATTTTTGGTGGTGATAAGTTGAATTGTGCAAACCTTAAAAAGTAAGATCATGATAATTTTGAATTTTTGTGTTGCTTTATTGTAATAATAGTGGGGATCTTTAATTAAATGAAGAGCCTTTATGATGGATATTCATCAATTTATAGTGCTATTATTATTTGATTTGTTGCAAAAGTAGATGATTGATAAAGATCATGCTTTTTTGTTTTTATTTTCAAGTTGTTGTTACTGTTTCTGAATATACTCCATTATTTTATTTTGTTGTTGCTTTAATCCATTTGTACACAATCAAAGCTTAATCAGATATGAATCTTCCTTCACTTAATTCCTTTACACATCATTATTTTGTCATGTACCTAATTTATAATATTTTTTTAAAGGATAAAATAGTTTTTCATGATAAGTACTTAACAAAAGTTTGTGTGCCATTACCTTGTTCACATAATAAAGTTAACATTTAAAATATTTATTTTTTAATAAGTTGTTGATATGAAAATTCTGAAATTTCTGGTTATCAATTTACCTGAGAAGCTGGTAAAAAATTAGCAGTTAAAAATTTGTGTTTAAAATCAAGTTAAGATTGCTAGTTTCAAGCATCATGCATGACTTAAAGTGTATGTTTGGTTTCATGGTAGAGAGTCAGAAGTGATTTCAGACACATAAAACTGATTTAAAGATGCTTGGATGATGTTGATTGGAATTGATTTTGCCACTAGAAAATATTCCAATAGAATTTGTAGCTTTTGACAATTAAGGTGATTTTTATTATCAATTTTTTGTTCAGGTGAAGGGTTTATTACATTGTTATCAAATTATTTTTCAACTTGCTTTTAGGTGTATGTTTTTTGTATCCAAACATAAACACTTCACATTCAACTAACTTTTAACTAAAATTAATTTTACAAAATCATTTTTTGTCACCGCAAAATCAAACACATACTAGTTTATTAAGTGTTTTTTTTCTTTTTCTGAAGTTTTTGATTATGTTCTGACTTTTGCCTTTGCCTACTTTCTCTGTTTCAGGGAAACAACGCAAGGTGGCCGAGTATTACAAGAAACAAGAGAGACTCCTCGAAGGATTTAATGAAATGGAAGCTATGCATGAAACTGGTTTTTTCCCAGGAGGTCTCTCTGAGGTGATTGTCTATTAATCACTATGATTTACTTACAATTTTCTTATAAACGGAGATGCATTTAACTAGTTTTGATGACTGTAATGTCACTGCAAAACTGAGATTTTTAGCGTGTCAATTGCGGATTGCGGAAAATATCGGTTTGTGCATATTCTGCTATGCTACAGTGCTATAGCACTATTATAACCACTATTTGACCACACTTTATACTAAATCGTGTATTGCCGAATAATAACGGTTTGTTTAAATTTACTATGCTATAGTGCTATAGCACCCCTATTTGATAACATTGGTGATTTCCAATTGAACCTAATCGATTCAAACACATGTAGATGAATGATTGAATTTAGTCGATAAGAGAATTCTACTGAATAAAATTGCATTATGTTTGTCAGGATGAAATGAAGCAACTTGCAAAGAGTGAGAGGACGGCAGTACTTGTGTCAAATGCATGTAACTTGGTGTTGTTTGCAGCAAAGGTTTTCGCCTCAATCGAGAGCAGATCATTAGCAGTCATTGCATCAACACTAGACTCTCTTTTGGATCTCTTGTCTGGTTTCATATTGTGGTTTACTTCCAATGCCATGAGCAAGCCAAACCGATATCACTATCCGATTGGAAAGAAACGCATGCAACCGGTGGTAGGACAACTTAGATTTTAAACTGCAACGTGATTGATGAGTCGTTTAAAATTTTAGTAAATGCAGTAGTTTCATAATACACTGAGTGTGAAACACAAATACTTATGTTTCTGTGCAGGGTATCATTGTTTTTGCATCCGTGATGGCAACCTTGGGCTTGCAGATTTTGATCGAGTCCGGTCGCCAAATTTTTGCAAAGGTAATCACAGATCAGCTTTTCTTGTTATTATTTGTTCACTAGTTTAAATTTGAGTTTAATCGTTTTTGTCGTGTGTAGCAGTGTTATACATTTCTCAGTATCATTCTTTTTCTTACAGGCTAAGCCAGAAGTCGATCCAGTAAAGGTGAATTGGATGATCGGGATTATGGTATTTGTAACTGTAGTGAAGTTCATTCTTATGATCTATTGTCGAAGATTCAAAAATGAAATTGTCAGAGCATATGCACAAGATCATTTTTTCGATGTCATTACTAATTCCGTCGGATTAGCCGCCGCTGTTCTAGCTGTCAAGTTCTTCTGGTGGATGGATCCAACAGGAGCTATTCTTGTAAGTTTATAACTTCATCTTATTTTCTTCGCATTTCAGTGTGGTTTTCTGATTGTGTTAACGTGTTCCACAATTAGTATTTTTCCATTAATAAATTTCCCGTAACTCCACAATGGGTGTAAACTCCTTTCGTATAAGCCACATCAAAATGTTGTCGAAGGGGGTTTTGTGTCACATGATTGTTTGCTGGTTAGAGGGTGATGTCTGGTGCAACAGTAAGGTTATTGTCTTGTGACCTAGAAGCAGTGTTTTAAAAATTGAACCGAACATCGAACTGGCGTGACCTTTGATTCGTGGTTCAACCGCCTTTAACTGCTCTCGAACTAGGACGAAACATCGATCAACACGCCAAAATAACTGACCATAGTAAAGGCCTGTTTAAGGTTTAATTAGTTCAACTGTTTTGGTTTGGTTTTTAAACATTGTCTGAAAGTTACAAATTCAGATCCTAGAATTTTTCGACCATTTTTCGGAAGCTTCATGCATTAAGCCGCCCTTTTTCCCGGAGTAATCCCCAAAGTTACTTCGGAAGTACACATTGAGAAAACAAACTCAATGTTTGTATAGCCCGGACGTGTACGTCTGGATAAAAGCACTTTTTTGTCATATGATTGTTGGTTACATCATACAAACTTCATGATTGACCTGCTAATGATGATTGTGTGATCCTGAATGTTCATTGCAGATTGCACTATATACAATCAACACATGGACAAAGACAGTGATGGAGAATGTCTGGTCACTCATTGGAAGAACAGCACCACCTGAATTCTTGGCAAAGTTAACATACCTTATATGGAACCACCATGAACAAATCAAGCACATAGATACAGTTAGAGCATACACGTTTGGTGCAGATTACTTTGTGGAAGTTGACATTGTTTTACCAGAAGACATGTTTCTCAACCAAGCACACAACATCGGCGAAACACTTCAAGAGAAGCTGGAACAACTTCCAGAAGTTGAAAGAGCTTTTGTTCACATTGATTTTGAGTTCACTCATAGACCTGAGCATAAGACATCAGTATGA
Protein sequence

>MS.gene045727.t1

MADSDSIRSEPLLDEVSDGSWRLNVKEFHLPSQNVVDHEKKSKFSFNGLLRKPRKQRKVAEYYKKQERLLEGFNEMEAMHETGFFPGGLSEDEMKQLAKSERTAVLVSNACNLVLFAAKVFASIESRSLAVIASTLDSLLDLLSGFILWFTSNAMSKPNRYHYPIGKKRMQPVGIIVFASVMATLGLQILIESGRQIFAKAKPEVDPVKVNWMIGIMVFVTVVKFILMIYCRRFKNEIVRAYAQDHFFDVITNSVGLAAAVLAVKFFWWMDPTGAILIALYTINTWTKTVMENVWSLIGRTAPPEFLAKLTYLIWNHHEQIKHIDTVRAYTFGADYFVEVDIVLPEDMFLNQAHNIGETLQEKLEQLPEVERAFVHIDFEFTHRPEHKTSV