Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04771.t1 | AET05287.1 | 94.6 | 147 | 8 | 0 | 6 | 152 | 67 | 213 | 1.50E-73 | 285.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04771.t1 | Q54UU1 | 54.4 | 125 | 56 | 1 | 15 | 138 | 3 | 127 | 3.2e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04771.t1 | G7L8Y7 | 94.6 | 147 | 8 | 0 | 6 | 152 | 67 | 213 | 1.1e-73 | 285.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04771 | MS.gene87621 | PPI |
| MS.gene85984 | MS.gene04771 | PPI |
| MS.gene068341 | MS.gene04771 | PPI |
| MS.gene96826 | MS.gene04771 | PPI |
| MS.gene59549 | MS.gene04771 | PPI |
| MS.gene031550 | MS.gene04771 | PPI |
| MS.gene006753 | MS.gene04771 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04771.t1 | MTR_8g103680 | 94.558 | 147 | 8 | 0 | 6 | 152 | 67 | 213 | 3.79e-104 | 296 |
| MS.gene04771.t1 | MTR_5g075330 | 86.667 | 150 | 20 | 0 | 3 | 152 | 25 | 174 | 3.87e-100 | 285 |
| MS.gene04771.t1 | MTR_5g075330 | 89.362 | 141 | 15 | 0 | 12 | 152 | 1 | 141 | 1.37e-97 | 277 |
| MS.gene04771.t1 | MTR_4g048530 | 29.787 | 141 | 84 | 3 | 18 | 143 | 26 | 166 | 8.66e-15 | 68.2 |
| MS.gene04771.t1 | MTR_8g095450 | 30.496 | 141 | 83 | 3 | 18 | 143 | 28 | 168 | 9.39e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04771.t1 | AT5G02280 | 87.234 | 141 | 18 | 0 | 12 | 152 | 1 | 141 | 1.29e-93 | 267 |
| MS.gene04771.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 4.65e-14 | 66.2 |
| MS.gene04771.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 4.65e-14 | 66.2 |
| MS.gene04771.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 4.65e-14 | 66.2 |
Find 36 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGTTGTCACCTGTTTC+TGG | 0.050534 | 4.2:+2076778 | MS.gene04771:CDS |
| TTTCCTGATATTTAGGATTA+TGG | 0.113471 | 4.2:+2076682 | MS.gene04771:intron |
| ATTTAGGATTATGGGTCTTC+TGG | 0.243224 | 4.2:+2076691 | MS.gene04771:intron |
| CTTTCCATTTGTTGAGTTCC+AGG | 0.279445 | 4.2:-2077380 | None:intergenic |
| TTCCTGATATTTAGGATTAT+GGG | 0.283975 | 4.2:+2076683 | MS.gene04771:intron |
| CAGAGTCTTGCAAAGGCTTT+GGG | 0.309508 | 4.2:+2075997 | MS.gene04771:CDS |
| TCAGAGTCTTGCAAAGGCTT+TGG | 0.325843 | 4.2:+2075996 | MS.gene04771:CDS |
| GAAGGACCGTGTTGCATTGC+TGG | 0.349606 | 4.2:+2077520 | MS.gene04771:CDS |
| CGGTGGATTGATCTACTATA+AGG | 0.397918 | 4.2:+2076059 | MS.gene04771:CDS |
| AGGCATCTCCATCTCATAGA+AGG | 0.407862 | 4.2:-2077453 | None:intergenic |
| GCTTACGGGTGGCTAGCTTA+TGG | 0.427608 | 4.2:+2076734 | MS.gene04771:CDS |
| AAATAATACCTGTCAGTGAC+TGG | 0.443726 | 4.2:-2076852 | None:intergenic |
| GGAGACCAATGATAGCTTAC+GGG | 0.449156 | 4.2:+2076720 | MS.gene04771:CDS |
| ATTCCTGCGCAACCAGAAAC+AGG | 0.450165 | 4.2:-2076790 | None:intergenic |
| TGAACCTGGAACTCAACAAA+TGG | 0.475337 | 4.2:+2077376 | MS.gene04771:CDS |
| TCAAAGAGCTCACATCGTAT+AGG | 0.480843 | 4.2:-2077473 | None:intergenic |
| AGCCACCCGTAAGCTATCAT+TGG | 0.486041 | 4.2:-2076725 | None:intergenic |
| CATTGCTTCCAGTCACTGAC+AGG | 0.492920 | 4.2:+2076844 | MS.gene04771:CDS |
| TGGAGACCAATGATAGCTTA+CGG | 0.498347 | 4.2:+2076719 | MS.gene04771:CDS |
| GGCATCTCCATCTCATAGAA+GGG | 0.506472 | 4.2:-2077452 | None:intergenic |
| GAAGAACCCCTTCTATGAGA+TGG | 0.515244 | 4.2:+2077445 | MS.gene04771:CDS |
| TCACCTGTTTCTGGTTGCGC+AGG | 0.516213 | 4.2:+2076787 | MS.gene04771:CDS |
| CTTTGATATCAACTTAGCTC+AGG | 0.519060 | 4.2:+2077490 | MS.gene04771:CDS |
| AAGGACCGTGTTGCATTGCT+GGG | 0.527169 | 4.2:+2077521 | MS.gene04771:CDS |
| GAAGATCAAATGTGTCAGCT+TGG | 0.530412 | 4.2:-2076822 | None:intergenic |
| CTTTACATCATCAATAAGTC+CGG | 0.534062 | 4.2:+2076039 | MS.gene04771:CDS |
| CTTAGCTCAGGCAGTGCAGA+AGG | 0.554879 | 4.2:+2077502 | MS.gene04771:CDS |
| GGCATTTCAGAGTCTTGCAA+AGG | 0.559105 | 4.2:+2075990 | MS.gene04771:CDS |
| TTATGGGTCTTCTGGACGAA+TGG | 0.564139 | 4.2:+2076699 | MS.gene04771:CDS |
| TACATCATCAATAAGTCCGG+TGG | 0.589559 | 4.2:+2076042 | MS.gene04771:CDS |
| AGGTGACAACTGCTGAGAGA+TGG | 0.604194 | 4.2:-2076770 | None:intergenic |
| GCATCTCCATCTCATAGAAG+GGG | 0.643808 | 4.2:-2077451 | None:intergenic |
| GACCAATGATAGCTTACGGG+TGG | 0.656334 | 4.2:+2076723 | MS.gene04771:CDS |
| AAAGGCTTTGGGAATGACAG+CGG | 0.659638 | 4.2:+2076008 | MS.gene04771:CDS |
| CTTATAGTAGATCAATCCAC+CGG | 0.673585 | 4.2:-2076058 | None:intergenic |
| ATCGACCCAGCAATGCAACA+CGG | 0.698434 | 4.2:-2077526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAATACTAAAAAATTT+AGG | + | chr4.2:2076307-2076326 | MS.gene04771:intron | 10.0% |
| !! | GAAAGTTTAGTAATAATTAT+TGG | + | chr4.2:2077073-2077092 | MS.gene04771:intron | 15.0% |
| !!! | TTATTTTTATTGTAAGCTTA+AGG | - | chr4.2:2076418-2076437 | None:intergenic | 15.0% |
| !! | CTTAACATAATAATGAGTTA+TGG | - | chr4.2:2076879-2076898 | None:intergenic | 20.0% |
| !!! | TGTTTAATTTCCTGATATTT+AGG | + | chr4.2:2076675-2076694 | MS.gene04771:intron | 20.0% |
| !!! | TTCAGTAAAACATTTTTCAT+AGG | - | chr4.2:2076157-2076176 | None:intergenic | 20.0% |
| ! | AAAAACTTTCTGAGTTAGAT+GGG | - | chr4.2:2076582-2076601 | None:intergenic | 25.0% |
| ! | GGTAAGAATTCAAACATAAT+TGG | - | chr4.2:2077180-2077199 | None:intergenic | 25.0% |
| ! | TAAAAACTTTCTGAGTTAGA+TGG | - | chr4.2:2076583-2076602 | None:intergenic | 25.0% |
| ! | TGTGAATAAAAAGCAAACAT+TGG | - | chr4.2:2076980-2076999 | None:intergenic | 25.0% |
| !! | AAAGTTTTTACTACTGTTGT+TGG | + | chr4.2:2076593-2076612 | MS.gene04771:intron | 25.0% |
| !! | ATTTAAAATCTACTAGTGCT+AGG | - | chr4.2:2077303-2077322 | None:intergenic | 25.0% |
| !! | TTCCTGATATTTAGGATTAT+GGG | + | chr4.2:2076683-2076702 | MS.gene04771:intron | 25.0% |
| !! | TTTCCTGATATTTAGGATTA+TGG | + | chr4.2:2076682-2076701 | MS.gene04771:intron | 25.0% |
| !!! | TGATTTTTGGTTTTAGGATA+TGG | + | chr4.2:2076350-2076369 | MS.gene04771:intron | 25.0% |
| !!! | TGTTTGCTTTTTATTCACAT+TGG | + | chr4.2:2076981-2077000 | MS.gene04771:intron | 25.0% |
| AACTTTCTCTCCTTAAATGT+TGG | - | chr4.2:2077060-2077079 | None:intergenic | 30.0% | |
| AAGAATGAATGCCAAAAATC+TGG | + | chr4.2:2076926-2076945 | MS.gene04771:intron | 30.0% | |
| CAATTAAGGTGACAAATCTA+CGG | - | chr4.2:2077201-2077220 | None:intergenic | 30.0% | |
| CTTTACATCATCAATAAGTC+CGG | + | chr4.2:2076039-2076058 | MS.gene04771:CDS | 30.0% | |
| TACATACAGGTAAAATTCCA+AGG | - | chr4.2:2077116-2077135 | None:intergenic | 30.0% | |
| TCACGTCTTAGATGAAAATA+AGG | + | chr4.2:2076375-2076394 | MS.gene04771:intron | 30.0% | |
| TTTGTCAAGGCTATTAATCA+AGG | - | chr4.2:2076521-2076540 | None:intergenic | 30.0% | |
| ! | AATAAGTTGGGTTGTTAATC+AGG | + | chr4.2:2076198-2076217 | MS.gene04771:intron | 30.0% |
| ! | AGTGATATGTACCAGATTTT+TGG | - | chr4.2:2076940-2076959 | None:intergenic | 30.0% |
| ! | CAAACATAATTGGTAACAAC+TGG | - | chr4.2:2077170-2077189 | None:intergenic | 30.0% |
| AAATAATACCTGTCAGTGAC+TGG | - | chr4.2:2076855-2076874 | None:intergenic | 35.0% | |
| AATTAATTCTTCTCTGCCAC+AGG | + | chr4.2:2077332-2077351 | MS.gene04771:intron | 35.0% | |
| ACCCAACTTATTCATCAAAG+AGG | - | chr4.2:2076190-2076209 | None:intergenic | 35.0% | |
| ATCTGGTACATATCACTGAT+TGG | + | chr4.2:2076943-2076962 | MS.gene04771:intron | 35.0% | |
| ATTAATTCTTCTCTGCCACA+GGG | + | chr4.2:2077333-2077352 | MS.gene04771:intron | 35.0% | |
| CCCAACTTATTCATCAAAGA+GGG | - | chr4.2:2076189-2076208 | None:intergenic | 35.0% | |
| CCCTCTTTGATGAATAAGTT+GGG | + | chr4.2:2076186-2076205 | MS.gene04771:intron | 35.0% | |
| CGTAGATTTGTCACCTTAAT+TGG | + | chr4.2:2077199-2077218 | MS.gene04771:intron | 35.0% | |
| CTTATAGTAGATCAATCCAC+CGG | - | chr4.2:2076061-2076080 | None:intergenic | 35.0% | |
| CTTTGATATCAACTTAGCTC+AGG | + | chr4.2:2077490-2077509 | MS.gene04771:CDS | 35.0% | |
| GACCCATAATCCTAAATATC+AGG | - | chr4.2:2076688-2076707 | None:intergenic | 35.0% | |
| TACTAGTGCTAGGTTATAGA+TGG | - | chr4.2:2077293-2077312 | None:intergenic | 35.0% | |
| TGTAATGCCTTGGTCATTTA+AGG | + | chr4.2:2077132-2077151 | MS.gene04771:intron | 35.0% | |
| TTAATCAGGTCACTTGAACT+TGG | + | chr4.2:2076212-2076231 | MS.gene04771:intron | 35.0% | |
| TTCACATTGGTAAGTAACCT+TGG | + | chr4.2:2076994-2077013 | MS.gene04771:intron | 35.0% | |
| TTTACCTGTATGTAATGCCT+TGG | + | chr4.2:2077122-2077141 | MS.gene04771:intron | 35.0% | |
| ! | ATTTAGGATTATGGGTCTTC+TGG | + | chr4.2:2076691-2076710 | MS.gene04771:intron | 35.0% |
| ! | CTGGGTTTTACCAACATTTA+AGG | + | chr4.2:2077047-2077066 | MS.gene04771:intron | 35.0% |
| ! | TAGCCTTGACAAATTCACAA+TGG | + | chr4.2:2076528-2076547 | MS.gene04771:intron | 35.0% |
| !! | TAACATAGTCTTATGTGCCT+TGG | + | chr4.2:2077096-2077115 | MS.gene04771:intron | 35.0% |
| !!! | CGTCGTTGATTTTTGGTTTT+AGG | + | chr4.2:2076344-2076363 | MS.gene04771:intron | 35.0% |
| !!! | CTATTCACGTCGTTGATTTT+TGG | + | chr4.2:2076337-2076356 | MS.gene04771:intron | 35.0% |
| ACATTGGTAAGTAACCTTGG+AGG | + | chr4.2:2076997-2077016 | MS.gene04771:intron | 40.0% | |
| ACGCCATTGTGAATTTGTCA+AGG | - | chr4.2:2076534-2076553 | None:intergenic | 40.0% | |
| GAAGATCAAATGTGTCAGCT+TGG | - | chr4.2:2076825-2076844 | None:intergenic | 40.0% | |
| GCCCTCTTTGATGAATAAGT+TGG | + | chr4.2:2076185-2076204 | MS.gene04771:intron | 40.0% | |
| TACATCATCAATAAGTCCGG+TGG | + | chr4.2:2076042-2076061 | MS.gene04771:CDS | 40.0% | |
| TAGTCTCTACTCTCTACTGT+GGG | + | chr4.2:2076109-2076128 | MS.gene04771:intron | 40.0% | |
| TCAAAGAGCTCACATCGTAT+AGG | - | chr4.2:2077476-2077495 | None:intergenic | 40.0% | |
| TGAACCTGGAACTCAACAAA+TGG | + | chr4.2:2077376-2077395 | MS.gene04771:CDS | 40.0% | |
| TGGAGACCAATGATAGCTTA+CGG | + | chr4.2:2076719-2076738 | MS.gene04771:CDS | 40.0% | |
| ! | ATGACCAAGGCATTACATAC+AGG | - | chr4.2:2077129-2077148 | None:intergenic | 40.0% |
| ! | CTTTCCATTTGTTGAGTTCC+AGG | - | chr4.2:2077383-2077402 | None:intergenic | 40.0% |
| ! | CTTTCTGAGTTAGATGGGTT+TGG | - | chr4.2:2076577-2076596 | None:intergenic | 40.0% |
| !! | CGGTGGATTGATCTACTATA+AGG | + | chr4.2:2076059-2076078 | MS.gene04771:CDS | 40.0% |
| AGGCATCTCCATCTCATAGA+AGG | - | chr4.2:2077456-2077475 | None:intergenic | 45.0% | |
| CAGAGTCTTGCAAAGGCTTT+GGG | + | chr4.2:2075997-2076016 | MS.gene04771:CDS | 45.0% | |
| CTAGTCTCTACTCTCTACTG+TGG | + | chr4.2:2076108-2076127 | MS.gene04771:intron | 45.0% | |
| GAAGAACCCCTTCTATGAGA+TGG | + | chr4.2:2077445-2077464 | MS.gene04771:CDS | 45.0% | |
| GAGTAGAGACTAGAGAGCAA+TGG | - | chr4.2:2076101-2076120 | None:intergenic | 45.0% | |
| GCATCTCCATCTCATAGAAG+GGG | - | chr4.2:2077454-2077473 | None:intergenic | 45.0% | |
| GGAGACCAATGATAGCTTAC+GGG | + | chr4.2:2076720-2076739 | MS.gene04771:CDS | 45.0% | |
| GGCATCTCCATCTCATAGAA+GGG | - | chr4.2:2077455-2077474 | None:intergenic | 45.0% | |
| GGCATTTCAGAGTCTTGCAA+AGG | + | chr4.2:2075990-2076009 | MS.gene04771:CDS | 45.0% | |
| GTGAACGCAAAGGCCAATTA+AGG | - | chr4.2:2077215-2077234 | None:intergenic | 45.0% | |
| TCAGAGTCTTGCAAAGGCTT+TGG | + | chr4.2:2075996-2076015 | MS.gene04771:CDS | 45.0% | |
| TTATGGGTCTTCTGGACGAA+TGG | + | chr4.2:2076699-2076718 | MS.gene04771:CDS | 45.0% | |
| ! | CAAGGCACCTTAAATGACCA+AGG | - | chr4.2:2077142-2077161 | None:intergenic | 45.0% |
| ! | TTTTTCGTGGTCTGTGAACC+TGG | + | chr4.2:2077362-2077381 | MS.gene04771:CDS | 45.0% |
| !! | AAAGGCTTTGGGAATGACAG+CGG | + | chr4.2:2076008-2076027 | MS.gene04771:CDS | 45.0% |
| AAGGACCGTGTTGCATTGCT+GGG | + | chr4.2:2077521-2077540 | MS.gene04771:CDS | 50.0% | |
| AGCCACCCGTAAGCTATCAT+TGG | - | chr4.2:2076728-2076747 | None:intergenic | 50.0% | |
| AGGTGACAACTGCTGAGAGA+TGG | - | chr4.2:2076773-2076792 | None:intergenic | 50.0% | |
| ATCGACCCAGCAATGCAACA+CGG | - | chr4.2:2077529-2077548 | None:intergenic | 50.0% | |
| ATTCCTGCGCAACCAGAAAC+AGG | - | chr4.2:2076793-2076812 | None:intergenic | 50.0% | |
| CACGAAAAACTTCGTCCCTG+TGG | - | chr4.2:2077351-2077370 | None:intergenic | 50.0% | |
| CAGCAGTTGTCACCTGTTTC+TGG | + | chr4.2:2076778-2076797 | MS.gene04771:CDS | 50.0% | |
| CAGCATCACAGTGAACGCAA+AGG | - | chr4.2:2077225-2077244 | None:intergenic | 50.0% | |
| CATTGCTTCCAGTCACTGAC+AGG | + | chr4.2:2076844-2076863 | MS.gene04771:CDS | 50.0% | |
| GACCAATGATAGCTTACGGG+TGG | + | chr4.2:2076723-2076742 | MS.gene04771:CDS | 50.0% | |
| TGGTAACAACTGGTGCAGCA+AGG | - | chr4.2:2077160-2077179 | None:intergenic | 50.0% | |
| ! | CACAGGGACGAAGTTTTTCG+TGG | + | chr4.2:2077349-2077368 | MS.gene04771:intron | 50.0% |
| CTTAGCTCAGGCAGTGCAGA+AGG | + | chr4.2:2077502-2077521 | MS.gene04771:CDS | 55.0% | |
| GAAGGACCGTGTTGCATTGC+TGG | + | chr4.2:2077520-2077539 | MS.gene04771:CDS | 55.0% | |
| GTACTGTGCAGTCTGTGCTG+TGG | + | chr4.2:2077024-2077043 | MS.gene04771:intron | 55.0% | |
| TGCACAGTACAGTGCCTCCA+AGG | - | chr4.2:2077014-2077033 | None:intergenic | 55.0% | |
| !! | GCTTACGGGTGGCTAGCTTA+TGG | + | chr4.2:2076734-2076753 | MS.gene04771:CDS | 55.0% |
| !! | TCACCTGTTTCTGGTTGCGC+AGG | + | chr4.2:2076787-2076806 | MS.gene04771:CDS | 55.0% |
| GTGCAGTCTGTGCTGTGGCT+GGG | + | chr4.2:2077029-2077048 | MS.gene04771:intron | 60.0% | |
| TGTGCAGTCTGTGCTGTGGC+TGG | + | chr4.2:2077028-2077047 | MS.gene04771:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 2075988 | 2077550 | 2075988 | ID=MS.gene04771 |
| chr4.2 | mRNA | 2075988 | 2077550 | 2075988 | ID=MS.gene04771.t1;Parent=MS.gene04771 |
| chr4.2 | exon | 2075988 | 2076080 | 2075988 | ID=MS.gene04771.t1.exon1;Parent=MS.gene04771.t1 |
| chr4.2 | CDS | 2075988 | 2076080 | 2075988 | ID=cds.MS.gene04771.t1;Parent=MS.gene04771.t1 |
| chr4.2 | exon | 2076697 | 2076865 | 2076697 | ID=MS.gene04771.t1.exon2;Parent=MS.gene04771.t1 |
| chr4.2 | CDS | 2076697 | 2076865 | 2076697 | ID=cds.MS.gene04771.t1;Parent=MS.gene04771.t1 |
| chr4.2 | exon | 2077354 | 2077550 | 2077354 | ID=MS.gene04771.t1.exon3;Parent=MS.gene04771.t1 |
| chr4.2 | CDS | 2077354 | 2077550 | 2077354 | ID=cds.MS.gene04771.t1;Parent=MS.gene04771.t1 |
| Gene Sequence |
| Protein sequence |
