AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048336

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048336.t1 MTR_8g068890 97.785 316 6 1 1 316 1 315 0.0 630
MS.gene048336.t1 MTR_5g032420 81.366 322 54 4 1 316 1 322 1.64e-178 496
MS.gene048336.t1 MTR_3g100570 74.763 317 75 3 1 316 1 313 2.55e-173 483
MS.gene048336.t1 MTR_8g086070 38.941 321 150 6 3 316 18 299 8.12e-67 211
MS.gene048336.t1 MTR_7g066690 36.977 311 167 5 6 314 17 300 2.76e-61 197
MS.gene048336.t1 MTR_4g018750 36.422 313 170 6 6 316 15 300 1.62e-60 195
MS.gene048336.t1 MTR_1g090330 33.333 303 176 4 11 312 19 296 6.73e-54 178
MS.gene048336.t1 MTR_3g462140 33.013 312 183 3 4 314 13 299 2.81e-50 169
MS.gene048336.t1 MTR_4g018750 34.783 276 151 6 6 279 15 263 5.00e-44 152
MS.gene048336.t1 MTR_4g018750 28.324 173 119 3 144 311 31 203 2.02e-15 75.5
MS.gene048336.t1 MTR_8g086070 38.961 231 98 5 3 229 18 209 2.23e-43 148
MS.gene048336.t1 MTR_1g088995 30.945 307 179 4 6 312 33 306 1.87e-41 146
MS.gene048336.t1 MTR_1g088995 29.596 223 125 3 6 228 33 223 3.25e-21 90.9
MS.gene048336.t1 MTR_4g057850 25.402 311 190 9 7 309 23 299 1.21e-18 85.1
MS.gene048336.t1 MTR_4g057850 28.426 197 120 4 115 303 9 192 8.68e-13 68.2
MS.gene048336.t1 MTR_1g087840 26.441 295 188 9 22 313 35 303 1.79e-16 79.0
MS.gene048336.t1 MTR_4g012850 22.684 313 201 6 6 313 16 292 2.78e-16 78.2
MS.gene048336.t1 MTR_4g012850 28.910 211 115 6 9 217 117 294 5.52e-16 77.4
MS.gene048336.t1 MTR_8g027975 24.484 339 193 12 4 313 49 353 1.46e-15 76.6
MS.gene048336.t1 MTR_1g097890 24.359 312 178 7 4 313 49 304 2.24e-15 75.9
MS.gene048336.t1 MTR_5g009500 21.557 334 207 8 4 313 36 338 5.64e-15 75.1
MS.gene048336.t1 MTR_5g055410 27.483 302 176 12 22 313 37 305 3.43e-14 72.8
MS.gene048336.t1 MTR_2g102010 24.579 297 182 6 4 294 15 275 3.58e-14 72.0
MS.gene048336.t1 MTR_2g102010 25.397 189 113 4 22 208 126 288 8.90e-11 62.0
MS.gene048336.t1 MTR_3g104500 22.340 282 196 7 44 314 53 322 8.62e-14 71.2
MS.gene048336.t1 MTR_4g119770 24.036 337 206 12 5 316 17 328 1.06e-13 71.2
MS.gene048336.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.49e-13 69.3
MS.gene048336.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 1.97e-13 69.7
MS.gene048336.t1 MTR_5g073040 25.159 314 191 7 11 316 40 317 2.03e-13 70.1
MS.gene048336.t1 MTR_4g088545 28.495 186 124 4 130 310 14 195 2.81e-13 69.7
MS.gene048336.t1 MTR_4g088545 23.006 326 204 9 2 313 8 300 8.12e-12 65.5
MS.gene048336.t1 MTR_7g109135 26.214 309 197 9 9 313 25 306 4.48e-13 69.3
MS.gene048336.t1 MTR_5g030530 25.723 311 197 8 9 313 14 296 7.18e-13 68.2
MS.gene048336.t1 MTR_4g114320 24.490 245 168 6 76 315 164 396 8.81e-13 68.9
MS.gene048336.t1 MTR_6g091800 25.943 212 133 6 115 311 5 207 1.23e-12 67.8
MS.gene048336.t1 MTR_8g096050 24.923 325 178 11 6 313 227 502 2.85e-12 67.4
MS.gene048336.t1 MTR_8g092080 22.642 318 180 9 4 315 50 307 5.93e-12 65.9
MS.gene048336.t1 MTR_8g076760 22.801 307 195 9 21 314 29 306 6.87e-12 65.5
MS.gene048336.t1 MTR_7g068340 30.337 178 105 6 144 315 103 267 8.16e-12 65.9
MS.gene048336.t1 MTR_8g066975 26.183 317 188 12 9 313 14 296 1.25e-11 64.7
MS.gene048336.t1 MTR_7g109135 25.890 309 191 10 9 313 25 299 3.30e-11 63.5
MS.gene048336.t1 MTR_6g034515 24.590 305 167 9 21 310 82 338 4.04e-11 63.5
MS.gene048336.t1 MTR_3g098460 25.309 324 182 12 4 313 203 480 4.83e-11 63.5
MS.gene048336.t1 MTR_7g064980 21.515 330 209 11 3 316 22 317 4.92e-11 63.2
MS.gene048336.t1 MTR_1g012030 23.457 243 170 6 76 313 179 410 5.28e-11 63.5
MS.gene048336.t1 MTR_7g117030 24.465 327 212 10 12 315 23 337 8.27e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048336.t1 AT4G24570 74.214 318 75 2 1 316 1 313 5.05e-169 472
MS.gene048336.t1 AT2G22500 75.316 316 73 1 1 316 1 311 6.31e-166 464
MS.gene048336.t1 AT5G09470 59.882 339 107 6 1 315 1 334 4.17e-125 361
MS.gene048336.t1 AT5G19760 39.498 319 147 5 3 314 15 294 6.08e-70 219
MS.gene048336.t1 AT5G58970 38.535 314 160 6 6 313 12 298 3.52e-65 207
MS.gene048336.t1 AT3G54110 37.898 314 168 6 4 316 13 300 2.89e-63 202
MS.gene048336.t1 AT1G14140 33.987 306 171 4 11 312 21 299 4.06e-52 174
MS.gene048336.t1 AT1G14140 27.094 203 122 2 9 211 121 297 3.12e-13 69.7
MS.gene048336.t1 AT4G03115 32.797 311 171 5 3 312 35 308 1.40e-46 160
MS.gene048336.t1 AT4G03115 32.476 311 172 5 3 312 67 340 2.50e-46 160
MS.gene048336.t1 AT5G58970 37.751 249 129 5 6 248 12 240 5.02e-44 152
MS.gene048336.t1 AT5G58970 26.590 173 122 3 144 311 32 204 2.60e-18 83.6
MS.gene048336.t1 AT4G03115 33.858 254 155 4 60 312 4 245 3.22e-42 146
MS.gene048336.t1 AT4G03115 34.583 240 144 4 74 312 10 237 8.80e-41 142
MS.gene048336.t1 AT4G03115 30.396 227 121 4 3 228 35 225 1.80e-24 99.4
MS.gene048336.t1 AT4G03115 30.396 227 121 4 3 228 35 225 2.37e-24 99.0
MS.gene048336.t1 AT5G01340 26.433 314 194 8 7 314 18 300 3.77e-22 94.7
MS.gene048336.t1 AT5G01340 27.869 183 125 3 122 303 11 187 2.11e-11 64.3
MS.gene048336.t1 AT1G25380 26.910 301 184 9 22 315 37 308 3.46e-16 78.6
MS.gene048336.t1 AT1G25380 26.910 301 184 9 22 315 37 308 3.46e-16 78.6
MS.gene048336.t1 AT5G56450 25.100 251 165 7 75 314 88 326 7.41e-16 77.4
MS.gene048336.t1 AT2G33820 24.848 330 191 11 5 313 13 306 1.01e-15 76.6
MS.gene048336.t1 AT5G46800 26.332 319 188 12 9 313 14 299 1.84e-15 75.9
MS.gene048336.t1 AT5G46800 22.535 213 145 3 110 313 1 202 5.75e-11 62.8
MS.gene048336.t1 AT5G46800 26.332 319 188 12 9 313 14 299 1.84e-15 75.9
MS.gene048336.t1 AT5G46800 22.535 213 145 3 110 313 1 202 5.75e-11 62.8
MS.gene048336.t1 AT5G46800 26.332 319 188 12 9 313 14 299 1.84e-15 75.9
MS.gene048336.t1 AT5G46800 22.535 213 145 3 110 313 1 202 5.75e-11 62.8
MS.gene048336.t1 AT3G53940 24.465 327 195 10 3 313 70 360 1.91e-15 76.6
MS.gene048336.t1 AT1G34065 24.204 314 180 7 4 315 80 337 3.23e-15 75.9
MS.gene048336.t1 AT1G34065 23.567 314 182 6 4 315 73 330 3.37e-15 75.5
MS.gene048336.t1 AT4G01100 25.000 340 190 12 4 313 40 344 4.67e-15 75.5
MS.gene048336.t1 AT4G01100 24.779 339 206 13 4 313 40 358 1.49e-14 73.9
MS.gene048336.t1 AT4G01100 26.792 265 157 9 78 313 38 294 7.81e-14 71.2
MS.gene048336.t1 AT2G33820 24.848 330 199 12 5 313 13 314 2.13e-13 70.1
MS.gene048336.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.97e-13 69.7
MS.gene048336.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.97e-13 69.7
MS.gene048336.t1 AT4G39460 23.871 310 182 5 4 313 56 311 2.97e-13 69.7
MS.gene048336.t1 AT2G37890 22.118 321 208 8 4 313 43 332 6.86e-13 68.9
MS.gene048336.t1 AT3G53940 26.425 193 125 4 132 312 73 260 8.81e-13 68.2
MS.gene048336.t1 AT2G35800 25.159 314 184 10 3 314 542 806 1.53e-12 68.6
MS.gene048336.t1 AT2G47490 27.213 305 172 12 22 313 33 300 1.67e-12 67.4
MS.gene048336.t1 AT5G66380 28.718 195 121 8 130 316 12 196 2.31e-12 67.0
MS.gene048336.t1 AT1G07030 26.380 326 183 12 6 316 36 319 2.56e-12 67.0
MS.gene048336.t1 AT4G26180 24.537 216 143 7 109 311 1 209 4.15e-12 66.2
MS.gene048336.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.95e-12 65.5
MS.gene048336.t1 AT3G55640 25.000 196 130 5 132 315 38 228 4.95e-12 65.5
MS.gene048336.t1 AT3G51870 28.977 176 116 4 141 315 104 271 6.63e-12 66.2
MS.gene048336.t1 AT3G51870 25.000 296 180 11 20 313 105 360 1.48e-11 65.1
MS.gene048336.t1 AT3G55640 24.747 198 136 4 126 315 36 228 6.68e-12 65.9
MS.gene048336.t1 AT3G55640 24.747 198 136 4 126 315 36 228 6.68e-12 65.9
MS.gene048336.t1 AT4G26180 24.537 216 143 7 109 311 1 209 7.57e-12 65.5
MS.gene048336.t1 AT2G33820 23.596 267 146 9 5 243 13 249 1.06e-11 64.7
MS.gene048336.t1 AT4G27940 24.340 341 217 10 12 314 71 408 1.17e-11 65.5
MS.gene048336.t1 AT5G17400 23.793 290 185 7 6 287 14 275 4.40e-11 63.2
MS.gene048336.t1 AT5G01500 29.121 182 110 8 140 315 131 299 4.64e-11 63.5
MS.gene048336.t1 AT3G21390 23.476 328 197 10 9 307 22 324 6.28e-11 62.8
MS.gene048336.t1 AT5G51050 27.513 189 126 5 132 316 223 404 8.58e-11 63.2
MS.gene048336.t1 AT3G21390 23.476 328 197 10 9 307 44 346 9.00e-11 62.8

Find 128 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCCTATGACGAGTTTA+AGG 0.260144 8.4:+34480739 MS.gene048336:CDS
GGGCTGCTCGCCGGTGGGAT+TGG 0.301666 8.4:+34480518 MS.gene048336:CDS
TGGCGCGGCGGTCGGGAATC+CGG 0.321453 8.4:+34480538 MS.gene048336:CDS
CGGCCCAACACGGGGCGGTT+TGG 0.326403 8.4:-34480312 None:intergenic
GTGGGATTGGCGCGGCGGTC+GGG 0.359376 8.4:+34480531 MS.gene048336:CDS
CGGATTTATAGTTTCTTCTC+TGG 0.361423 8.4:-34480610 None:intergenic
TGCTAATGCTGGTCGGATAT+TGG 0.367208 8.4:-34480249 None:intergenic
ACAGGTTCGTAAGTTGCTTA+AGG 0.385790 8.4:+34481048 MS.gene048336:CDS
CGGCCAAACCGCCCCGTGTT+GGG 0.388767 8.4:+34480309 MS.gene048336:CDS
GGTGGGATTGGCGCGGCGGT+CGG 0.398701 8.4:+34480530 MS.gene048336:CDS
GTGCTGCTACACCTTCTTGT+TGG 0.410225 8.4:-34480358 None:intergenic
GGGTCCTATGGCTCTTTACA+AGG 0.411226 8.4:+34480964 MS.gene048336:CDS
TTGCCGTTGACAAGCAAGAT+TGG 0.420958 8.4:+34480491 MS.gene048336:CDS
ACAAACAACACAACGGTAAA+CGG 0.423325 8.4:-34481016 None:intergenic
GTTGTTTGTTACATTGGAAC+AGG 0.435604 8.4:+34481030 MS.gene048336:CDS
GGCATAGCTTCCATCATTGC+AGG 0.435640 8.4:+34480155 MS.gene048336:CDS
ACGGCCAAACCGCCCCGTGT+TGG 0.435675 8.4:+34480308 MS.gene048336:CDS
GGTTGGATGAAGGATGGGCT+TGG 0.436837 8.4:+34480779 MS.gene048336:CDS
GGCGGTTTGGCCGTCACCAC+CGG 0.437029 8.4:-34480299 None:intergenic
GGTCCCTGCCTTGTAATTGT+AGG 0.442748 8.4:-34480995 None:intergenic
ATTGGAGCGGGGCTGCTCGC+CGG 0.443449 8.4:+34480509 MS.gene048336:CDS
TCCAACCCGGTTCGGTTCAT+CGG 0.448540 8.4:+34480273 MS.gene048336:CDS
CGTTGTGTTGTTTGTTACAT+TGG 0.449852 8.4:+34481024 MS.gene048336:CDS
CTCTATTCCACCACCCGTAT+GGG 0.452624 8.4:+34480419 MS.gene048336:CDS
GGATTTATAGTTTCTTCTCT+GGG 0.452868 8.4:-34480609 None:intergenic
CGGCGACCGATGAACCGAAC+CGG 0.453026 8.4:-34480279 None:intergenic
TCCCTCGTCTTTCGCCATCC+TGG 0.455818 8.4:-34480648 None:intergenic
GTTAGTTACCGCGTCCCAGC+TGG 0.456033 8.4:+34480718 MS.gene048336:CDS
CAATCAATGGCACCGGAGTA+AGG 0.460053 8.4:-34480917 None:intergenic
GGAGTAAGGCGGAGGCGATC+CGG 0.461442 8.4:-34480903 None:intergenic
TCATCGGTCGCCGGTGGTGA+CGG 0.462318 8.4:+34480289 MS.gene048336:CDS
GTCTTTCGCCATCCTGGTGA+TGG 0.464552 8.4:-34480642 None:intergenic
GGAGCGGGGCTGCTCGCCGG+TGG 0.465110 8.4:+34480512 MS.gene048336:CDS
GTTGGATGAAGGATGGGCTT+GGG 0.467370 8.4:+34480780 MS.gene048336:CDS
TTGACAAGCAAGATTGGAGC+GGG 0.472465 8.4:+34480497 MS.gene048336:CDS
ATTAGCATTCCAACCCGGTT+CGG 0.477760 8.4:+34480265 MS.gene048336:CDS
CGGTGGATGTGATTAAGACT+AGG 0.482921 8.4:+34480858 MS.gene048336:CDS
GTGTCTCCGCCACCGTTCTC+CGG 0.485350 8.4:+34480390 MS.gene048336:CDS
TTGAAGACTGTTCGTGCTGA+GGG 0.486561 8.4:+34480944 MS.gene048336:CDS
GACCGCCGCGCCAATCCCAC+CGG 0.488627 8.4:-34480528 None:intergenic
TATTCCTACAATTACAAGGC+AGG 0.491525 8.4:+34480991 MS.gene048336:CDS
TGTTCGTGCTGAGGGTCCTA+TGG 0.492843 8.4:+34480952 MS.gene048336:CDS
TGTTGAACACCCTGCAATGA+TGG 0.493211 8.4:-34480165 None:intergenic
AAGGTTCGAATGCAGCTTCA+AGG 0.496054 8.4:+34480206 MS.gene048336:CDS
CAACAGCGATCGGCCCAACA+CGG 0.500458 8.4:-34480322 None:intergenic
AACAGCGATCGGCCCAACAC+GGG 0.504249 8.4:-34480321 None:intergenic
GCTGGTCGGATATTGGTTGT+AGG 0.505194 8.4:-34480242 None:intergenic
TCTCTATTCCACCACCCGTA+TGG 0.506394 8.4:+34480418 MS.gene048336:CDS
GGCGGCGGTTGCGTCCAATC+CGG 0.509572 8.4:+34480838 MS.gene048336:CDS
ATGAATATGAAGGTGGAGGC+CGG 0.509995 8.4:+34480884 MS.gene048336:CDS
CTTAATCACATCCACCGGAT+TGG 0.511289 8.4:-34480852 None:intergenic
CCGTTGTCGACGCCATCACC+AGG 0.516545 8.4:+34480630 MS.gene048336:CDS
AACGGCAAAGTACCGGAGAT+AGG 0.516962 8.4:-34480476 None:intergenic
CGGCTGATGTGGCAATGGTT+AGG 0.521248 8.4:+34480558 MS.gene048336:CDS
AGTCTTCAAAGCACAATCAA+TGG 0.523057 8.4:-34480930 None:intergenic
ATAGAGTCCCATACGGGTGG+TGG 0.523945 8.4:-34480426 None:intergenic
GAGCGGGGCTGCTCGCCGGT+GGG 0.525418 8.4:+34480513 MS.gene048336:CDS
GGTCGGGAATCCGGCTGATG+TGG 0.525455 8.4:+34480547 MS.gene048336:CDS
ACGAGGGAGTTACTAGCCTC+TGG 0.527867 8.4:+34480663 MS.gene048336:CDS
CTAACCATTGCCACATCAGC+CGG 0.528201 8.4:-34480557 None:intergenic
ACCGATGAACCGAACCGGGT+TGG 0.533148 8.4:-34480274 None:intergenic
AAATGGTCTGATCCTATCTC+CGG 0.538460 8.4:+34480464 MS.gene048336:CDS
AGTAATCGCAATGGGTGTGA+AGG 0.538899 8.4:+34480118 None:intergenic
CGTGTTGGGCCGATCGCTGT+TGG 0.539682 8.4:+34480323 MS.gene048336:CDS
GCTTGTCAACGGCAAAGTAC+CGG 0.541832 8.4:-34480483 None:intergenic
TTTGAAGACTGTTCGTGCTG+AGG 0.545035 8.4:+34480943 MS.gene048336:CDS
AGCTTAACACCAACAGCGAT+CGG 0.547313 8.4:-34480332 None:intergenic
CTCGTCATAGGATGCCAGCT+GGG 0.548714 8.4:-34480732 None:intergenic
GGCGACCGATGAACCGAACC+GGG 0.549411 8.4:-34480278 None:intergenic
TGTGGCAATGGTTAGGATGC+AGG 0.550254 8.4:+34480565 MS.gene048336:CDS
AAACCCTTGTAAAGAGCCAT+AGG 0.551305 8.4:-34480968 None:intergenic
CGGTTCGGTTCATCGGTCGC+CGG 0.554937 8.4:+34480280 MS.gene048336:CDS
GAGAACGGTGGCGGAGACAC+CGG 0.556496 8.4:-34480387 None:intergenic
ACTCGTCATAGGATGCCAGC+TGG 0.556535 8.4:-34480733 None:intergenic
CTGGGACGCGGTAACTAACA+TGG 0.558044 8.4:-34480714 None:intergenic
GCTCGCCGGTGGGATTGGCG+CGG 0.558280 8.4:+34480523 MS.gene048336:CDS
ACACTGCCGGAGAACGGTGG+CGG 0.561241 8.4:-34480396 None:intergenic
CCTGGTGATGGCGTCGACAA+CGG 0.566152 8.4:-34480630 None:intergenic
CGCCGGTGGGATTGGCGCGG+CGG 0.566467 8.4:+34480526 MS.gene048336:CDS
GGATCGCCTCCGCCTTACTC+CGG 0.567757 8.4:+34480905 MS.gene048336:CDS
TAGTTTCTTCTCTGGGCCGA+CGG 0.570902 8.4:-34480602 None:intergenic
CCGGGTTGGAATGCTAATGC+TGG 0.573037 8.4:-34480260 None:intergenic
GGTGTGAAGGGTTTCGTCGA+AGG 0.574034 8.4:+34480131 MS.gene048336:CDS
ATGGCACCGGAGTAAGGCGG+AGG 0.575695 8.4:-34480911 None:intergenic
TTCGGTTCATCGGTCGCCGG+TGG 0.575971 8.4:+34480283 MS.gene048336:CDS
ATTCCTACAATTACAAGGCA+GGG 0.578803 8.4:+34480992 MS.gene048336:CDS
ATAGGATGCCAGCTGGGACG+CGG 0.579724 8.4:-34480726 None:intergenic
GGTGGATGTGATTAAGACTA+GGG 0.581075 8.4:+34480859 MS.gene048336:CDS
GGTCCTATGGCTCTTTACAA+GGG 0.581509 8.4:+34480965 MS.gene048336:CDS
GTAATCGCAATGGGTGTGAA+GGG 0.582693 8.4:+34480119 None:intergenic
TCATATCATAGAGTCCCATA+CGG 0.584545 8.4:-34480433 None:intergenic
GGTGATGAATATGAAGGTGG+AGG 0.589447 8.4:+34480880 MS.gene048336:CDS
GGCGGTTGCGTCCAATCCGG+TGG 0.592377 8.4:+34480841 MS.gene048336:CDS
CAAAGCACAATCAATGGCAC+CGG 0.593300 8.4:-34480924 None:intergenic
CATATCATAGAGTCCCATAC+GGG 0.597212 8.4:-34480432 None:intergenic
GACTAGGGTGATGAATATGA+AGG 0.600049 8.4:+34480874 MS.gene048336:CDS
CACCAGGATGGCGAAAGACG+AGG 0.603337 8.4:+34480646 MS.gene048336:CDS
CTAGTCTTAATCACATCCAC+CGG 0.603477 8.4:-34480857 None:intergenic
GAATCCGGCTGATGTGGCAA+TGG 0.605065 8.4:+34480553 MS.gene048336:CDS
ACACCCTCTTGATCTCATCA+AGG 0.606884 8.4:+34480187 MS.gene048336:CDS
GTGAAGGGTTTCGTCGAAGG+AGG 0.607310 8.4:+34480134 MS.gene048336:CDS
CGAACCTTGATGAGATCAAG+AGG 0.611493 8.4:-34480191 None:intergenic
ACAGCGATCGGCCCAACACG+GGG 0.612038 8.4:-34480320 None:intergenic
GGCCGACGGAAGTCTTCCGT+CGG 0.612610 8.4:-34480588 None:intergenic
GTTAAGCTAGTCCAACAAGA+AGG 0.614148 8.4:+34480347 MS.gene048336:CDS
ATAGAGACACTGCCGGAGAA+CGG 0.615334 8.4:-34480402 None:intergenic
AAAATGGTTGGATGAAGGAT+GGG 0.619691 8.4:+34480774 MS.gene048336:CDS
ACCAGGATGGCGAAAGACGA+GGG 0.622914 8.4:+34480647 MS.gene048336:CDS
ATGGTTAGGATGCAGGCCGA+CGG 0.622979 8.4:+34480572 MS.gene048336:CDS
TAGGGTGATGAATATGAAGG+TGG 0.625045 8.4:+34480877 MS.gene048336:CDS
GGCCGACGGAAGACTTCCGT+CGG 0.629424 8.4:+34480586 MS.gene048336:CDS
GCTCCAATCTTGCTTGTCAA+CGG 0.630329 8.4:-34480494 None:intergenic
CCAGCATTAGCATTCCAACC+CGG 0.630776 8.4:+34480260 MS.gene048336:CDS
TGGTGGAATAGAGACACTGC+CGG 0.636597 8.4:-34480409 None:intergenic
TGTCGACGCCATCACCAGGA+TGG 0.643772 8.4:+34480634 MS.gene048336:CDS
TAAGAGATGAACCGCGCCAG+AGG 0.646828 8.4:-34480679 None:intergenic
GCATAGCTTCCATCATTGCA+GGG 0.651032 8.4:+34480156 MS.gene048336:CDS
GTTGGAATGCTAATGCTGGT+CGG 0.653431 8.4:-34480256 None:intergenic
ATCATAGAGTCCCATACGGG+TGG 0.658486 8.4:-34480429 None:intergenic
ACGCGGTAACTAACATGGCG+CGG 0.664454 8.4:-34480709 None:intergenic
GTTGACAAGCAAGATTGGAG+CGG 0.666908 8.4:+34480496 MS.gene048336:CDS
GCGATCGGCCCAACACGGGG+CGG 0.686402 8.4:-34480317 None:intergenic
GAGACACTGCCGGAGAACGG+TGG 0.687440 8.4:-34480399 None:intergenic
CAATGTAACAAACAACACAA+CGG 0.699064 8.4:-34481023 None:intergenic
GAACCTTGATGAGATCAAGA+GGG 0.713345 8.4:-34480190 None:intergenic
TGACAAGCAAGATTGGAGCG+GGG 0.724268 8.4:+34480498 MS.gene048336:CDS
TCAATGGCACCGGAGTAAGG+CGG 0.732141 8.4:-34480914 None:intergenic
GGAGTTACTAGCCTCTGGCG+CGG 0.739969 8.4:+34480668 MS.gene048336:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATATGATGAAGAAAAAA+TGG + chr8.4:34480447-34480466 MS.gene048336:CDS 20.0%
!!! AAAAGATTTTGAAAAATGGT+TGG + chr8.4:34480762-34480781 MS.gene048336:CDS 20.0%
!!! AAGGAAAAGATTTTGAAAAA+TGG + chr8.4:34480758-34480777 MS.gene048336:CDS 20.0%
!!! GTTTTATTCCTACAATTACA+AGG + chr8.4:34480987-34481006 MS.gene048336:CDS 25.0%
CAATGTAACAAACAACACAA+CGG - chr8.4:34481026-34481045 None:intergenic 30.0%
GGATTTATAGTTTCTTCTCT+GGG - chr8.4:34480612-34480631 None:intergenic 30.0%
TTTGAAAAATGGTTGGATGA+AGG + chr8.4:34480769-34480788 MS.gene048336:CDS 30.0%
AAAAATGGTTGGATGAAGGA+TGG + chr8.4:34480773-34480792 MS.gene048336:CDS 35.0%
AAAATGGTTGGATGAAGGAT+GGG + chr8.4:34480774-34480793 MS.gene048336:CDS 35.0%
ACAAACAACACAACGGTAAA+CGG - chr8.4:34481019-34481038 None:intergenic 35.0%
AGTCTTCAAAGCACAATCAA+TGG - chr8.4:34480933-34480952 None:intergenic 35.0%
ATTCCTACAATTACAAGGCA+GGG + chr8.4:34480992-34481011 MS.gene048336:CDS 35.0%
CGGATTTATAGTTTCTTCTC+TGG - chr8.4:34480613-34480632 None:intergenic 35.0%
TATTCCTACAATTACAAGGC+AGG + chr8.4:34480991-34481010 MS.gene048336:CDS 35.0%
TCATATCATAGAGTCCCATA+CGG - chr8.4:34480436-34480455 None:intergenic 35.0%
! CTTTTCCTTAAACTCGTCAT+AGG - chr8.4:34480747-34480766 None:intergenic 35.0%
! GTTGTTTGTTACATTGGAAC+AGG + chr8.4:34481030-34481049 MS.gene048336:CDS 35.0%
!! CGTTGTGTTGTTTGTTACAT+TGG + chr8.4:34481024-34481043 MS.gene048336:CDS 35.0%
AAACCCTTGTAAAGAGCCAT+AGG - chr8.4:34480971-34480990 None:intergenic 40.0%
AAATGGTCTGATCCTATCTC+CGG + chr8.4:34480464-34480483 MS.gene048336:CDS 40.0%
ACAGGTTCGTAAGTTGCTTA+AGG + chr8.4:34481048-34481067 MS.gene048336:CDS 40.0%
CATATCATAGAGTCCCATAC+GGG - chr8.4:34480435-34480454 None:intergenic 40.0%
GAACCTTGATGAGATCAAGA+GGG - chr8.4:34480193-34480212 None:intergenic 40.0%
GACTAGGGTGATGAATATGA+AGG + chr8.4:34480874-34480893 MS.gene048336:CDS 40.0%
GGTGGATGTGATTAAGACTA+GGG + chr8.4:34480859-34480878 MS.gene048336:CDS 40.0%
GTTAAGCTAGTCCAACAAGA+AGG + chr8.4:34480347-34480366 MS.gene048336:CDS 40.0%
TAGGGTGATGAATATGAAGG+TGG + chr8.4:34480877-34480896 MS.gene048336:CDS 40.0%
!! CTAGTCTTAATCACATCCAC+CGG - chr8.4:34480860-34480879 None:intergenic 40.0%
AAGGTTCGAATGCAGCTTCA+AGG + chr8.4:34480206-34480225 MS.gene048336:CDS 45.0%
ACACCCTCTTGATCTCATCA+AGG + chr8.4:34480187-34480206 MS.gene048336:CDS 45.0%
AGCTTAACACCAACAGCGAT+CGG - chr8.4:34480335-34480354 None:intergenic 45.0%
ATGAATATGAAGGTGGAGGC+CGG + chr8.4:34480884-34480903 MS.gene048336:CDS 45.0%
ATTAGCATTCCAACCCGGTT+CGG + chr8.4:34480265-34480284 MS.gene048336:CDS 45.0%
CAAAGCACAATCAATGGCAC+CGG - chr8.4:34480927-34480946 None:intergenic 45.0%
CGAACCTTGATGAGATCAAG+AGG - chr8.4:34480194-34480213 None:intergenic 45.0%
CGGTGGATGTGATTAAGACT+AGG + chr8.4:34480858-34480877 MS.gene048336:CDS 45.0%
CTTAATCACATCCACCGGAT+TGG - chr8.4:34480855-34480874 None:intergenic 45.0%
GCATAGCTTCCATCATTGCA+GGG + chr8.4:34480156-34480175 MS.gene048336:CDS 45.0%
GCTCCAATCTTGCTTGTCAA+CGG - chr8.4:34480497-34480516 None:intergenic 45.0%
GGCATCCTATGACGAGTTTA+AGG + chr8.4:34480739-34480758 MS.gene048336:CDS 45.0%
GGTCCTATGGCTCTTTACAA+GGG + chr8.4:34480965-34480984 MS.gene048336:CDS 45.0%
GGTGATGAATATGAAGGTGG+AGG + chr8.4:34480880-34480899 MS.gene048336:CDS 45.0%
GTTGACAAGCAAGATTGGAG+CGG + chr8.4:34480496-34480515 MS.gene048336:CDS 45.0%
GTTGGAATGCTAATGCTGGT+CGG - chr8.4:34480259-34480278 None:intergenic 45.0%
TGCTAATGCTGGTCGGATAT+TGG - chr8.4:34480252-34480271 None:intergenic 45.0%
TGTTGAACACCCTGCAATGA+TGG - chr8.4:34480168-34480187 None:intergenic 45.0%
TTGAAGACTGTTCGTGCTGA+GGG + chr8.4:34480944-34480963 MS.gene048336:CDS 45.0%
TTGACAAGCAAGATTGGAGC+GGG + chr8.4:34480497-34480516 MS.gene048336:CDS 45.0%
TTGCCGTTGACAAGCAAGAT+TGG + chr8.4:34480491-34480510 MS.gene048336:CDS 45.0%
TTTGAAGACTGTTCGTGCTG+AGG + chr8.4:34480943-34480962 MS.gene048336:CDS 45.0%
!!! GAGTTTTTCGGCTGGTTTTG+TGG + chr8.4:34480817-34480836 MS.gene048336:CDS 45.0%
!!! GGTGTAGCAGCACTTTTTTC+CGG + chr8.4:34480368-34480387 MS.gene048336:CDS 45.0%
!!! TTTTTCGGCTGGTTTTGTGG+CGG + chr8.4:34480820-34480839 MS.gene048336:CDS 45.0%
ATAGAGACACTGCCGGAGAA+CGG - chr8.4:34480405-34480424 None:intergenic 50.0%
ATCATAGAGTCCCATACGGG+TGG - chr8.4:34480432-34480451 None:intergenic 50.0%
CCAGCATTAGCATTCCAACC+CGG + chr8.4:34480260-34480279 MS.gene048336:CDS 50.0%
CGAAAAACTCGCTGTCACGT+GGG - chr8.4:34480807-34480826 None:intergenic 50.0%
CTAACCATTGCCACATCAGC+CGG - chr8.4:34480560-34480579 None:intergenic 50.0%
CTCTATTCCACCACCCGTAT+GGG + chr8.4:34480419-34480438 MS.gene048336:CDS 50.0%
GCTTGTCAACGGCAAAGTAC+CGG - chr8.4:34480486-34480505 None:intergenic 50.0%
GGCATAGCTTCCATCATTGC+AGG + chr8.4:34480155-34480174 MS.gene048336:CDS 50.0%
GGGTCCTATGGCTCTTTACA+AGG + chr8.4:34480964-34480983 MS.gene048336:CDS 50.0%
GGTCCCTGCCTTGTAATTGT+AGG - chr8.4:34480998-34481017 None:intergenic 50.0%
TAGTTTCTTCTCTGGGCCGA+CGG - chr8.4:34480605-34480624 None:intergenic 50.0%
TCTCTATTCCACCACCCGTA+TGG + chr8.4:34480418-34480437 MS.gene048336:CDS 50.0%
TGACAAGCAAGATTGGAGCG+GGG + chr8.4:34480498-34480517 MS.gene048336:CDS 50.0%
TGGTGGAATAGAGACACTGC+CGG - chr8.4:34480412-34480431 None:intergenic 50.0%
TGTGGCAATGGTTAGGATGC+AGG + chr8.4:34480565-34480584 MS.gene048336:CDS 50.0%
! CAATCAATGGCACCGGAGTA+AGG - chr8.4:34480920-34480939 None:intergenic 50.0%
! CCACGTGACAGCGAGTTTTT+CGG + chr8.4:34480805-34480824 MS.gene048336:CDS 50.0%
! GCTGGTCGGATATTGGTTGT+AGG - chr8.4:34480245-34480264 None:intergenic 50.0%
! GTGCTGCTACACCTTCTTGT+TGG - chr8.4:34480361-34480380 None:intergenic 50.0%
! GTTGGATGAAGGATGGGCTT+GGG + chr8.4:34480780-34480799 MS.gene048336:CDS 50.0%
!! AACGGCAAAGTACCGGAGAT+AGG - chr8.4:34480479-34480498 None:intergenic 50.0%
ACCAGGATGGCGAAAGACGA+GGG + chr8.4:34480647-34480666 MS.gene048336:CDS 55.0%
ACGCGGTAACTAACATGGCG+CGG - chr8.4:34480712-34480731 None:intergenic 55.0%
ACTCGTCATAGGATGCCAGC+TGG - chr8.4:34480736-34480755 None:intergenic 55.0%
ATAGAGTCCCATACGGGTGG+TGG - chr8.4:34480429-34480448 None:intergenic 55.0%
ATGGTTAGGATGCAGGCCGA+CGG + chr8.4:34480572-34480591 MS.gene048336:CDS 55.0%
CCGAAAAACTCGCTGTCACG+TGG - chr8.4:34480808-34480827 None:intergenic 55.0%
CGGCTGATGTGGCAATGGTT+AGG + chr8.4:34480558-34480577 MS.gene048336:CDS 55.0%
CTCGTCATAGGATGCCAGCT+GGG - chr8.4:34480735-34480754 None:intergenic 55.0%
CTGGGACGCGGTAACTAACA+TGG - chr8.4:34480717-34480736 None:intergenic 55.0%
GAATCCGGCTGATGTGGCAA+TGG + chr8.4:34480553-34480572 MS.gene048336:CDS 55.0%
GGTGTGAAGGGTTTCGTCGA+AGG + chr8.4:34480131-34480150 MS.gene048336:CDS 55.0%
GTCTTTCGCCATCCTGGTGA+TGG - chr8.4:34480645-34480664 None:intergenic 55.0%
GTGAAGGGTTTCGTCGAAGG+AGG + chr8.4:34480134-34480153 MS.gene048336:CDS 55.0%
TAAGAGATGAACCGCGCCAG+AGG - chr8.4:34480682-34480701 None:intergenic 55.0%
TGTTCGTGCTGAGGGTCCTA+TGG + chr8.4:34480952-34480971 MS.gene048336:CDS 55.0%
! ACGAGGGAGTTACTAGCCTC+TGG + chr8.4:34480663-34480682 MS.gene048336:CDS 55.0%
! CCGGGTTGGAATGCTAATGC+TGG - chr8.4:34480263-34480282 None:intergenic 55.0%
! GGTTGGATGAAGGATGGGCT+TGG + chr8.4:34480779-34480798 MS.gene048336:CDS 55.0%
! TCAATGGCACCGGAGTAAGG+CGG - chr8.4:34480917-34480936 None:intergenic 55.0%
!! GTGACAGCGAGTTTTTCGGC+TGG + chr8.4:34480809-34480828 MS.gene048336:CDS 55.0%
!! TCCAACCCGGTTCGGTTCAT+CGG + chr8.4:34480273-34480292 MS.gene048336:CDS 55.0%
AACAGCGATCGGCCCAACAC+GGG - chr8.4:34480324-34480343 None:intergenic 60.0%
ACCGATGAACCGAACCGGGT+TGG - chr8.4:34480277-34480296 None:intergenic 60.0%
ATAGGATGCCAGCTGGGACG+CGG - chr8.4:34480729-34480748 None:intergenic 60.0%
CAACAGCGATCGGCCCAACA+CGG - chr8.4:34480325-34480344 None:intergenic 60.0%
CACCAGGATGGCGAAAGACG+AGG + chr8.4:34480646-34480665 MS.gene048336:CDS 60.0%
GTTAGTTACCGCGTCCCAGC+TGG + chr8.4:34480718-34480737 MS.gene048336:CDS 60.0%
TCCCTCGTCTTTCGCCATCC+TGG - chr8.4:34480651-34480670 None:intergenic 60.0%
TGTCGACGCCATCACCAGGA+TGG + chr8.4:34480634-34480653 MS.gene048336:CDS 60.0%
! GGAGTTACTAGCCTCTGGCG+CGG + chr8.4:34480668-34480687 MS.gene048336:CDS 60.0%
!! CCTGGTGATGGCGTCGACAA+CGG - chr8.4:34480633-34480652 None:intergenic 60.0%
!!! TTCGGCTGGTTTTGTGGCGG+CGG + chr8.4:34480823-34480842 MS.gene048336:CDS 60.0%
ACACTGCCGGAGAACGGTGG+CGG - chr8.4:34480399-34480418 None:intergenic 65.0%
ACAGCGATCGGCCCAACACG+GGG - chr8.4:34480323-34480342 None:intergenic 65.0%
CCGTTGTCGACGCCATCACC+AGG + chr8.4:34480630-34480649 MS.gene048336:CDS 65.0%
CGGCGACCGATGAACCGAAC+CGG - chr8.4:34480282-34480301 None:intergenic 65.0%
CGTGTTGGGCCGATCGCTGT+TGG + chr8.4:34480323-34480342 MS.gene048336:CDS 65.0%
GAGACACTGCCGGAGAACGG+TGG - chr8.4:34480402-34480421 None:intergenic 65.0%
GGAGTAAGGCGGAGGCGATC+CGG - chr8.4:34480906-34480925 None:intergenic 65.0%
GGATCGCCTCCGCCTTACTC+CGG + chr8.4:34480905-34480924 MS.gene048336:CDS 65.0%
GGCCGACGGAAGACTTCCGT+CGG + chr8.4:34480586-34480605 MS.gene048336:CDS 65.0%
GGCCGACGGAAGTCTTCCGT+CGG - chr8.4:34480591-34480610 None:intergenic 65.0%
GGCGACCGATGAACCGAACC+GGG - chr8.4:34480281-34480300 None:intergenic 65.0%
GGTCGGGAATCCGGCTGATG+TGG + chr8.4:34480547-34480566 MS.gene048336:CDS 65.0%
GTGTCTCCGCCACCGTTCTC+CGG + chr8.4:34480390-34480409 MS.gene048336:CDS 65.0%
TCATCGGTCGCCGGTGGTGA+CGG + chr8.4:34480289-34480308 MS.gene048336:CDS 65.0%
TTCGGTTCATCGGTCGCCGG+TGG + chr8.4:34480283-34480302 MS.gene048336:CDS 65.0%
! ATGGCACCGGAGTAAGGCGG+AGG - chr8.4:34480914-34480933 None:intergenic 65.0%
!! CGGTTCGGTTCATCGGTCGC+CGG + chr8.4:34480280-34480299 MS.gene048336:CDS 65.0%
!! GAGAACGGTGGCGGAGACAC+CGG - chr8.4:34480390-34480409 None:intergenic 65.0%
ACGGCCAAACCGCCCCGTGT+TGG + chr8.4:34480308-34480327 MS.gene048336:CDS 70.0%
ATTGGAGCGGGGCTGCTCGC+CGG + chr8.4:34480509-34480528 MS.gene048336:CDS 70.0%
CGGCCAAACCGCCCCGTGTT+GGG + chr8.4:34480309-34480328 MS.gene048336:CDS 70.0%
GGCGGCGGTTGCGTCCAATC+CGG + chr8.4:34480838-34480857 MS.gene048336:CDS 70.0%
GGCGGTTGCGTCCAATCCGG+TGG + chr8.4:34480841-34480860 MS.gene048336:CDS 70.0%
! GGCGGTTTGGCCGTCACCAC+CGG - chr8.4:34480302-34480321 None:intergenic 70.0%
CGGCCCAACACGGGGCGGTT+TGG - chr8.4:34480315-34480334 None:intergenic 75.0%
GACCGCCGCGCCAATCCCAC+CGG - chr8.4:34480531-34480550 None:intergenic 75.0%
GCGATCGGCCCAACACGGGG+CGG - chr8.4:34480320-34480339 None:intergenic 75.0%
GGGCTGCTCGCCGGTGGGAT+TGG + chr8.4:34480518-34480537 MS.gene048336:CDS 75.0%
TGGCGCGGCGGTCGGGAATC+CGG + chr8.4:34480538-34480557 MS.gene048336:CDS 75.0%
! GCTCGCCGGTGGGATTGGCG+CGG + chr8.4:34480523-34480542 MS.gene048336:CDS 75.0%
! GGTGGGATTGGCGCGGCGGT+CGG + chr8.4:34480530-34480549 MS.gene048336:CDS 75.0%
! GTGGGATTGGCGCGGCGGTC+GGG + chr8.4:34480531-34480550 MS.gene048336:CDS 75.0%
! GAGCGGGGCTGCTCGCCGGT+GGG + chr8.4:34480513-34480532 MS.gene048336:CDS 80.0%
!! CGCCGGTGGGATTGGCGCGG+CGG + chr8.4:34480526-34480545 MS.gene048336:CDS 80.0%
! GGAGCGGGGCTGCTCGCCGG+TGG + chr8.4:34480512-34480531 MS.gene048336:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 34480128 34481078 34480128 ID=MS.gene048336
chr8.4 mRNA 34480128 34481078 34480128 ID=MS.gene048336.t1;Parent=MS.gene048336
chr8.4 exon 34480128 34481078 34480128 ID=MS.gene048336.t1.exon1;Parent=MS.gene048336.t1
chr8.4 CDS 34480128 34481078 34480128 ID=cds.MS.gene048336.t1;Parent=MS.gene048336.t1
Gene Sequence

>MS.gene048336

ATGGGTGTGAAGGGTTTCGTCGAAGGAGGCATAGCTTCCATCATTGCAGGGTGTTCAACACACCCTCTTGATCTCATCAAGGTTCGAATGCAGCTTCAAGGAGAGAATAGTACTCCTACAACCAATATCCGACCAGCATTAGCATTCCAACCCGGTTCGGTTCATCGGTCGCCGGTGGTGACGGCCAAACCGCCCCGTGTTGGGCCGATCGCTGTTGGTGTTAAGCTAGTCCAACAAGAAGGTGTAGCAGCACTTTTTTCCGGTGTCTCCGCCACCGTTCTCCGGCAGTGTCTCTATTCCACCACCCGTATGGGACTCTATGATATGATGAAGAAAAAATGGTCTGATCCTATCTCCGGTACTTTGCCGTTGACAAGCAAGATTGGAGCGGGGCTGCTCGCCGGTGGGATTGGCGCGGCGGTCGGGAATCCGGCTGATGTGGCAATGGTTAGGATGCAGGCCGACGGAAGACTTCCGTCGGCCCAGAGAAGAAACTATAAATCCGTTGTCGACGCCATCACCAGGATGGCGAAAGACGAGGGAGTTACTAGCCTCTGGCGCGGTTCATCTCTTACAGTGAACCGCGCCATGTTAGTTACCGCGTCCCAGCTGGCATCCTATGACGAGTTTAAGGAAAAGATTTTGAAAAATGGTTGGATGAAGGATGGGCTTGGGACCCACGTGACAGCGAGTTTTTCGGCTGGTTTTGTGGCGGCGGTTGCGTCCAATCCGGTGGATGTGATTAAGACTAGGGTGATGAATATGAAGGTGGAGGCCGGATCGCCTCCGCCTTACTCCGGTGCCATTGATTGTGCTTTGAAGACTGTTCGTGCTGAGGGTCCTATGGCTCTTTACAAGGGTTTTATTCCTACAATTACAAGGCAGGGACCGTTTACCGTTGTGTTGTTTGTTACATTGGAACAGGTTCGTAAGTTGCTTAAGGATTTCTAA
Protein sequence

>MS.gene048336.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENSTPTTNIRPALAFQPGSVHRSPVVTAKPPRVGPIAVGVKLVQQEGVAALFSGVSATVLRQCLYSTTRMGLYDMMKKKWSDPISGTLPLTSKIGAGLLAGGIGAAVGNPADVAMVRMQADGRLPSAQRRNYKSVVDAITRMAKDEGVTSLWRGSSLTVNRAMLVTASQLASYDEFKEKILKNGWMKDGLGTHVTASFSAGFVAAVASNPVDVIKTRVMNMKVEAGSPPPYSGAIDCALKTVRAEGPMALYKGFIPTITRQGPFTVVLFVTLEQVRKLLKDF