AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048625

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048625.t1 MTR_7g095080 97.103 725 20 1 1 724 1 725 0.0 1455
MS.gene048625.t1 MTR_4g094992 92.664 518 32 1 213 724 2 519 0.0 989
MS.gene048625.t1 MTR_1g023985 45.609 706 326 6 20 724 542 1190 0.0 635
MS.gene048625.t1 MTR_1g114490 45.727 667 304 6 59 724 512 1121 0.0 610
MS.gene048625.t1 MTR_3g023880 44.964 685 317 8 43 724 407 1034 0.0 593
MS.gene048625.t1 MTR_6g061840 44.380 685 320 7 41 720 247 875 0.0 582
MS.gene048625.t1 MTR_2g015320 40.993 705 339 7 41 723 46 695 0.0 553
MS.gene048625.t1 MTR_5g075310 41.997 681 295 6 43 720 204 787 0.0 543
MS.gene048625.t1 MTR_1g086640 41.226 718 345 10 27 724 33 693 0.0 543
MS.gene048625.t1 MTR_3g054400 40.462 692 346 8 39 722 359 992 3.06e-178 538
MS.gene048625.t1 MTR_2g098440 40.260 693 348 8 39 723 562 1196 1.54e-175 536
MS.gene048625.t1 MTR_3g052610 40.027 742 340 12 27 722 33 715 2.79e-175 519
MS.gene048625.t1 MTR_6g061840 46.410 571 245 7 41 606 247 761 9.28e-169 504
MS.gene048625.t1 MTR_4g485610 40.211 664 323 9 78 724 49 655 1.55e-166 495
MS.gene048625.t1 MTR_8g080240 38.171 689 343 14 47 712 3 631 4.96e-155 464
MS.gene048625.t1 MTR_8g080240 38.171 689 343 14 47 712 3 631 2.74e-154 464
MS.gene048625.t1 MTR_8g080240 38.390 646 319 13 47 673 3 588 3.02e-144 434
MS.gene048625.t1 MTR_8g041990 35.855 661 253 10 43 699 101 594 9.94e-125 386
MS.gene048625.t1 MTR_6g061840 49.869 381 150 1 41 421 247 586 4.46e-124 382
MS.gene048625.t1 MTR_3g086770 33.287 715 369 21 41 717 255 899 5.24e-97 329
MS.gene048625.t1 MTR_3g014030 32.394 710 382 18 41 717 255 899 1.84e-96 328
MS.gene048625.t1 MTR_0267s0070 32.943 683 362 18 66 717 937 1554 1.32e-91 314
MS.gene048625.t1 MTR_1g056490 30.663 724 416 17 45 712 228 921 2.42e-79 274
MS.gene048625.t1 MTR_1543s0010 41.026 312 162 6 327 633 10 304 4.50e-69 229
MS.gene048625.t1 MTR_4g023590 37.195 328 165 8 15 320 227 535 3.42e-55 205
MS.gene048625.t1 MTR_4g023590 38.624 189 114 2 332 519 692 879 3.90e-32 134
MS.gene048625.t1 MTR_3g055020 41.202 233 121 3 385 616 44 261 7.10e-53 189
MS.gene048625.t1 MTR_8g045110 28.406 433 288 7 288 712 105 523 6.67e-48 179
MS.gene048625.t1 MTR_1g109170 29.493 434 273 11 292 709 601 1017 7.07e-48 183
MS.gene048625.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 4.33e-32 134
MS.gene048625.t1 MTR_1g109170 29.630 432 271 11 294 709 603 1017 1.06e-47 182
MS.gene048625.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 4.66e-32 134
MS.gene048625.t1 MTR_8g106630 29.932 441 271 13 288 709 543 964 1.63e-46 179
MS.gene048625.t1 MTR_8g106630 35.294 238 110 3 35 269 260 456 2.21e-34 141
MS.gene048625.t1 MTR_1g088590 29.532 491 245 15 48 491 30 466 1.60e-43 169
MS.gene048625.t1 MTR_4g134790 33.243 370 216 9 280 630 888 1245 5.03e-42 165
MS.gene048625.t1 MTR_4g134790 33.096 281 133 6 7 273 562 801 1.78e-33 139
MS.gene048625.t1 MTR_4g134790 33.243 370 216 9 280 630 885 1242 5.70e-42 165
MS.gene048625.t1 MTR_4g134790 33.096 281 133 6 7 273 562 801 2.03e-33 139
MS.gene048625.t1 MTR_8g044260 28.341 434 281 10 291 709 521 939 5.21e-41 162
MS.gene048625.t1 MTR_8g044260 31.600 250 119 3 27 269 228 432 1.30e-30 129
MS.gene048625.t1 MTR_5g090430 28.241 432 273 12 273 682 526 942 5.72e-40 159
MS.gene048625.t1 MTR_5g090430 28.512 242 118 5 45 273 184 383 2.22e-25 113
MS.gene048625.t1 MTR_5g090430 28.241 432 273 12 273 682 413 829 7.53e-40 158
MS.gene048625.t1 MTR_5g090430 28.512 242 118 5 45 273 71 270 2.51e-25 112
MS.gene048625.t1 MTR_4g120070 32.734 278 129 7 77 320 208 461 1.33e-33 139
MS.gene048625.t1 MTR_4g120070 37.624 202 119 3 324 519 605 805 1.01e-32 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048625.t1 AT1G26370 70.349 688 202 1 35 720 26 713 0.0 1023
MS.gene048625.t1 AT3G26560 45.609 706 326 7 20 724 487 1135 0.0 634
MS.gene048625.t1 AT2G35340 44.476 715 335 9 13 724 371 1026 0.0 607
MS.gene048625.t1 AT2G35340 44.476 715 335 9 13 724 233 888 0.0 606
MS.gene048625.t1 AT1G32490 43.916 715 340 9 13 724 360 1016 0.0 604
MS.gene048625.t1 AT1G32490 43.916 715 340 9 13 724 370 1026 0.0 603
MS.gene048625.t1 AT4G16680 42.616 711 343 8 19 724 106 756 0.0 586
MS.gene048625.t1 AT3G62310 42.378 715 335 10 30 724 40 697 0.0 556
MS.gene048625.t1 AT2G47250 42.308 702 331 9 40 724 57 701 0.0 552
MS.gene048625.t1 AT4G18465 39.800 701 346 6 41 720 41 686 0.0 543
MS.gene048625.t1 AT5G13010 40.490 694 347 8 39 724 549 1184 1.90e-178 543
MS.gene048625.t1 AT3G62310 46.388 526 224 6 30 539 40 523 2.55e-152 454
MS.gene048625.t1 AT3G62310 45.372 551 239 8 14 545 25 532 6.03e-152 454
MS.gene048625.t1 AT4G16680 48.211 475 201 3 19 493 186 615 4.93e-151 454
MS.gene048625.t1 AT1G27900 36.797 693 358 14 47 715 3 639 4.77e-149 451
MS.gene048625.t1 AT1G27900 38.409 591 299 9 47 628 3 537 2.16e-140 423
MS.gene048625.t1 AT1G27900 38.409 591 299 9 47 628 3 537 4.59e-140 423
MS.gene048625.t1 AT5G10370 32.817 710 375 18 48 723 306 947 6.36e-96 327
MS.gene048625.t1 AT2G35920 30.748 722 418 18 45 712 167 860 9.31e-88 296
MS.gene048625.t1 AT4G01020 31.171 709 388 19 48 723 303 944 1.09e-87 302
MS.gene048625.t1 AT2G35920 30.748 722 418 18 45 712 225 918 2.02e-87 296
MS.gene048625.t1 AT2G35920 30.748 722 418 18 45 712 257 950 3.89e-87 296
MS.gene048625.t1 AT1G48650 29.625 746 449 19 16 709 267 988 5.93e-79 275
MS.gene048625.t1 AT1G48650 29.625 746 449 19 16 709 267 988 6.87e-79 275
MS.gene048625.t1 AT1G58050 29.726 693 382 24 14 636 561 1218 2.97e-69 248
MS.gene048625.t1 AT1G33390 38.621 290 136 5 40 312 233 497 1.05e-51 195
MS.gene048625.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.61e-38 153
MS.gene048625.t1 AT1G33390 38.621 290 136 5 40 312 233 497 1.05e-51 195
MS.gene048625.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.61e-38 153
MS.gene048625.t1 AT5G14900 34.615 286 160 8 448 724 3 270 3.36e-48 172
MS.gene048625.t1 AT2G01130 30.000 430 268 9 292 705 492 904 3.81e-48 184
MS.gene048625.t1 AT2G01130 34.553 246 117 3 27 269 199 403 6.65e-35 143
MS.gene048625.t1 AT2G01130 30.000 430 268 9 292 705 427 839 4.58e-48 183
MS.gene048625.t1 AT2G01130 34.553 246 117 3 27 269 134 338 6.38e-35 143
MS.gene048625.t1 AT1G58060 30.223 493 267 15 280 707 895 1375 2.92e-45 175
MS.gene048625.t1 AT1G58060 35.772 246 108 7 39 273 605 811 2.74e-35 144
MS.gene048625.t1 AT1G06670 28.788 396 252 9 247 630 524 901 1.85e-40 160
MS.gene048625.t1 AT1G06670 32.500 240 116 3 39 273 208 406 2.14e-31 132
MS.gene048625.t1 AT1G06670 28.788 396 252 9 247 630 524 901 1.97e-40 160
MS.gene048625.t1 AT1G06670 32.500 240 116 3 39 273 208 406 2.31e-31 132
MS.gene048625.t1 AT2G30800 27.673 477 292 15 274 716 184 641 1.85e-39 157
MS.gene048625.t1 AT2G30800 27.673 477 292 15 274 716 527 984 2.35e-39 157
MS.gene048625.t1 AT2G30800 30.417 240 121 3 39 273 178 376 1.36e-28 123
MS.gene048625.t1 AT5G04895 28.046 435 283 11 288 707 529 948 1.16e-38 155
MS.gene048625.t1 AT5G04895 30.315 254 125 3 27 273 239 447 1.73e-31 132
MS.gene048625.t1 AT2G47680 30.337 445 226 12 47 456 25 420 1.81e-38 154

Find 151 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGTGTTGATGACATATT+AGG 0.230492 7.4:+14189969 MS.gene048625:CDS
GCATCTACATAATCTGTTTC+TGG 0.232424 7.4:-14187133 None:intergenic
GCCTTTGATCCGGCTCCATC+TGG 0.237514 7.4:+14187611 MS.gene048625:CDS
TTCTAAGAAAACTGTCATTC+TGG 0.285262 7.4:-14186961 None:intergenic
TTGATTATTGTTGGTGAAAC+TGG 0.291242 7.4:+14185811 MS.gene048625:CDS
ATAAGAGCTTTGGCATAAAC+TGG 0.292906 7.4:-14192076 None:intergenic
CACAGCTGCATTGAGGAAAA+AGG 0.328575 7.4:-14192846 None:intergenic
TTATGCACTCTGGTTTCTTC+CGG 0.338992 7.4:-14193175 None:intergenic
AGGTTGCAACGCTTAATTTC+CGG 0.339311 7.4:-14189918 None:intergenic
TTCATGTTGGATAAAGATAA+TGG 0.353602 7.4:+14186938 MS.gene048625:CDS
AAGTCACTTCCTATTGCTTC+GGG 0.354749 7.4:+14185661 MS.gene048625:CDS
TCTTTGCAATGCCTGGGACT+TGG 0.361472 7.4:-14189017 None:intergenic
TGTTTACAGGGAAGCATTGT+TGG 0.379684 7.4:+14186751 MS.gene048625:intron
CCCTTGAACGTGAACAGCTT+TGG 0.383415 7.4:-14187074 None:intergenic
TAACAGAGTACCCAACCTTT+TGG 0.385983 7.4:-14186364 None:intergenic
TTCTTTACACAACAGGGCTT+TGG 0.389466 7.4:+14193109 MS.gene048625:intron
CTCGTGCGGAAAATAAAATA+TGG 0.390077 7.4:-14192164 None:intergenic
GAGACATCAATTACAATTCC+TGG 0.391735 7.4:+14188905 MS.gene048625:CDS
CATACCTTCTTTGCAATGCC+TGG 0.393486 7.4:-14189024 None:intergenic
TGTCCACACTGATGTCTTGA+TGG 0.399891 7.4:+14186829 MS.gene048625:CDS
AAAGTCACTTCCTATTGCTT+CGG 0.400688 7.4:+14185660 MS.gene048625:CDS
GATGGAAGAGCTGCATATAT+AGG 0.404918 7.4:-14187572 None:intergenic
CAACCTATGGTTAACTGAAC+TGG 0.411358 7.4:+14193256 MS.gene048625:CDS
TGGGTGTTGATGACATATTA+GGG 0.411725 7.4:+14189970 MS.gene048625:CDS
TCAAGGACATGTTCAACAAA+TGG 0.413696 7.4:+14192763 MS.gene048625:CDS
TCAATTACATACTTGATTCC+AGG 0.417163 7.4:-14188923 None:intergenic
AGCAAGTCAGTTTAACTGCT+TGG 0.419602 7.4:+14192102 MS.gene048625:CDS
AGAGATTAATTGAAGAGGTT+CGG 0.420010 7.4:+14185776 MS.gene048625:CDS
GGCAAACAACTCATGGTGTT+TGG 0.423711 7.4:+14185374 None:intergenic
TAGAGCAGCTACTGATTCAT+TGG 0.426806 7.4:+14192531 MS.gene048625:CDS
GCCAAAGCTGTTCACGTTCA+AGG 0.430027 7.4:+14187073 MS.gene048625:CDS
GACAGCTATTATAAAATCAT+TGG 0.430046 7.4:+14191967 MS.gene048625:CDS
CGGGCCATTTGACGTCCAAC+TGG 0.430443 7.4:-14192040 None:intergenic
TTGGCGTTGGCATTAGCATT+GGG 0.434102 7.4:-14185443 None:intergenic
TAACCAGAGTTGACAACCTA+TGG 0.434887 7.4:+14193243 MS.gene048625:CDS
ACTTGCTAAAATAAGAGCTT+TGG 0.436656 7.4:-14192086 None:intergenic
ATCCTTCGTCTGTATTGTTC+CGG 0.437198 7.4:+14193156 MS.gene048625:CDS
TTTGGTAACAGTGGGCGTGC+AGG 0.437226 7.4:+14189822 MS.gene048625:intron
CCTTTGATCCGGCTCCATCT+GGG 0.438053 7.4:+14187612 MS.gene048625:CDS
CAACTCACAACCACATTCTT+CGG 0.438554 7.4:-14186339 None:intergenic
TGACAAGGTTCTCACCTTGA+AGG 0.442041 7.4:-14190011 None:intergenic
TGCCATGACGGAAAAGTTGT+TGG 0.442723 7.4:+14186266 MS.gene048625:CDS
ATTGGCGTTGGCATTAGCAT+TGG 0.442749 7.4:-14185444 None:intergenic
AAGGACCTGGAAAATGCTTT+CGG 0.443930 7.4:+14189850 MS.gene048625:CDS
TTGGTTATTGCGATTGGCGT+TGG 0.445640 7.4:-14185456 None:intergenic
AACTGGGTCCAAGGGAAGTC+GGG 0.447934 7.4:-14192059 None:intergenic
GACCTGGAAAATTGTTATCA+AGG 0.449776 7.4:-14187158 None:intergenic
TATTCTTGTTCTCATTATCA+AGG 0.450410 7.4:-14186907 None:intergenic
ATTTCAATACCCGAAGCAAT+AGG 0.452539 7.4:-14185670 None:intergenic
GACAATTATAACAGAATACT+TGG 0.453394 7.4:-14186786 None:intergenic
CAGGGCTTTGGCAAGTGGTC+AGG 0.455322 7.4:+14193121 MS.gene048625:intron
GACATGCTCGTGACATTCAC+AGG 0.455685 7.4:+14192644 MS.gene048625:CDS
CGTGCAGGGCGTGAAGGACC+TGG 0.456345 7.4:+14189837 MS.gene048625:CDS
GTGAGTTGGGCCAAAAGGTT+GGG 0.456369 7.4:+14186354 MS.gene048625:CDS
ATGGGTTTGGTTATTGCGAT+TGG 0.457755 7.4:-14185462 None:intergenic
GTAATACCAGGGAGAGGAAT+GGG 0.458451 7.4:-14185480 None:intergenic
ATCAAGTATGTAATTGATCC+TGG 0.458843 7.4:+14188929 MS.gene048625:CDS
AGTCCCAGGCATTGCAAAGA+AGG 0.461418 7.4:+14189020 MS.gene048625:CDS
CGTACTTTCTCTGAGTACTT+TGG 0.462297 7.4:+14187046 MS.gene048625:CDS
GCCATGACGGAAAAGTTGTT+GGG 0.462870 7.4:+14186267 MS.gene048625:CDS
ACATTCTTCGGCAACTCGCT+TGG 0.465098 7.4:-14186327 None:intergenic
GAAGAATGTGGTTGTGAGTT+GGG 0.470574 7.4:+14186341 MS.gene048625:CDS
TGATCCAGTTGGACGTCAAA+TGG 0.470663 7.4:+14192036 MS.gene048625:CDS
TAACCATAGGTTGTCAACTC+TGG 0.470971 7.4:-14193246 None:intergenic
TGCTGTTGAACGGCTTATCA+AGG 0.477511 7.4:+14187502 MS.gene048625:CDS
CGAAGAATGTGGTTGTGAGT+TGG 0.477627 7.4:+14186340 MS.gene048625:CDS
GCTGCAAAATGACATTAGAA+AGG 0.479362 7.4:-14189938 None:intergenic
GATATACTAGTATTTCTGAC+TGG 0.479761 7.4:+14187464 MS.gene048625:CDS
AGAGTGATGTGGTCTCCCTC+TGG 0.480341 7.4:-14192496 None:intergenic
TCAAATGGCCCGACTTCCCT+TGG 0.483015 7.4:+14192051 MS.gene048625:CDS
ACCAGGGAGAGGAATGGGTT+TGG 0.484017 7.4:-14185475 None:intergenic
GGCTTTGGCAAGTGGTCAGG+TGG 0.485781 7.4:+14193124 MS.gene048625:CDS
CAAGGACATGTTCAACAAAT+GGG 0.487040 7.4:+14192764 MS.gene048625:CDS
GCTCGCATTTGCTGCTCAGA+TGG 0.488003 7.4:-14187590 None:intergenic
CGGTCAAATTCTATCAAAGA+TGG 0.488070 7.4:+14186878 MS.gene048625:CDS
ATATTTGATCCTTGTTGAGT+TGG 0.489249 7.4:-14186405 None:intergenic
AAAGCGCGTTCCTATGATCC+TGG 0.491588 7.4:+14188959 MS.gene048625:CDS
TGAGAAGAGATTAATTGAAG+AGG 0.492285 7.4:+14185771 MS.gene048625:CDS
TTGGTAACAGTGGGCGTGCA+GGG 0.495052 7.4:+14189823 MS.gene048625:intron
CGTTCCTATGATCCTGGAAA+AGG 0.496489 7.4:+14188965 MS.gene048625:CDS
ATTTCATTGAGAAACCTTCA+AGG 0.501014 7.4:+14189997 MS.gene048625:CDS
TAATTTCGACGAGCCACAAA+AGG 0.507055 7.4:-14185416 None:intergenic
GCAAATGCGAGCCTTTGATC+CGG 0.507905 7.4:+14187601 MS.gene048625:CDS
ACAGAATACTTGGAAAGATA+AGG 0.508465 7.4:-14186776 None:intergenic
TGTGAGTTGGGCCAAAAGGT+TGG 0.511660 7.4:+14186353 MS.gene048625:CDS
CGTTGGCATTAGCATTGGGT+TGG 0.512423 7.4:-14185439 None:intergenic
GGATACCTGTAGGTTCCATC+TGG 0.513099 7.4:-14192875 None:intergenic
ATACCTTCTTTGCAATGCCT+GGG 0.513868 7.4:-14189023 None:intergenic
TCGTTAAATATTATGCACTC+TGG 0.514440 7.4:-14193185 None:intergenic
ACTTTCTCTGAGTACTTTGG+TGG 0.515214 7.4:+14187049 MS.gene048625:CDS
CCCAGATGGAGCCGGATCAA+AGG 0.518326 7.4:-14187612 None:intergenic
AGGATCAAATATATGACAGA+TGG 0.520985 7.4:+14186416 MS.gene048625:CDS
CACAACAGGGCTTTGGCAAG+TGG 0.525131 7.4:+14193116 MS.gene048625:intron
CAAATTCTATCAAAGATGGT+TGG 0.530800 7.4:+14186882 MS.gene048625:CDS
ATTGTTGGTGAAACTGGAAG+TGG 0.530851 7.4:+14185817 MS.gene048625:CDS
ACCAAACCCATTCCTCTCCC+TGG 0.534233 7.4:+14185474 MS.gene048625:CDS
GGTCTGAAACTTGCTTCTTG+TGG 0.535848 7.4:+14192785 MS.gene048625:CDS
CCAAAGCTGTTCACGTTCAA+GGG 0.541798 7.4:+14187074 MS.gene048625:CDS
GTGATAGCAGCAACACGTCT+AGG 0.547288 7.4:-14186299 None:intergenic
ACTTGAGGATTCTACAATGC+CGG 0.554792 7.4:+14189899 MS.gene048625:CDS
GAGCCAGTTCAGTTAACCAT+AGG 0.554841 7.4:-14193259 None:intergenic
TTGCGAAACCCAGATGGAGC+CGG 0.557730 7.4:-14187620 None:intergenic
CGAGTATAAAATATATCCAC+AGG 0.558690 7.4:-14187106 None:intergenic
AAACTGGGTCCAAGGGAAGT+CGG 0.561694 7.4:-14192060 None:intergenic
AAATGAATTTGAGAAACTTG+AGG 0.562155 7.4:+14189884 MS.gene048625:CDS
TGGTTGTGAGTTGGGCCAAA+AGG 0.566424 7.4:+14186349 MS.gene048625:CDS
AAGAGCTTCGCAAGTCCAGA+GGG 0.566562 7.4:+14192481 MS.gene048625:CDS
GAATACGAATGGTTTCATGT+TGG 0.567599 7.4:+14186925 MS.gene048625:CDS
GGCTCCATCTGGGTTTCGCA+AGG 0.567724 7.4:+14187622 MS.gene048625:CDS
GACATGATGATTAACTTCAA+CGG 0.568214 7.4:-14187007 None:intergenic
AGTGGGCGTGCAGGGCGTGA+AGG 0.569161 7.4:+14189831 MS.gene048625:intron
CTATGATCCTGGAAAAGGCA+TGG 0.570476 7.4:+14188970 MS.gene048625:CDS
ATATACTAGTATTTCTGACT+GGG 0.570954 7.4:+14187465 MS.gene048625:CDS
GGAACAATACAGACGAAGGA+TGG 0.575517 7.4:-14193154 None:intergenic
TGACAGATGGGTTACTTCTG+AGG 0.582783 7.4:+14186429 MS.gene048625:CDS
AGTAATACCAGGGAGAGGAA+TGG 0.583151 7.4:-14185481 None:intergenic
ACCTCTTCGCACTTCTCGTG+CGG 0.584338 7.4:-14192178 None:intergenic
GCAGCTGTGAAGCAGCCAGA+TGG 0.589847 7.4:+14192860 MS.gene048625:CDS
AAGAGATTGATGCTGTTGAA+CGG 0.590120 7.4:+14187492 MS.gene048625:CDS
GTCCACACTGATGTCTTGAT+GGG 0.592239 7.4:+14186830 MS.gene048625:CDS
GGAAAAGTAATACCAGGGAG+AGG 0.595830 7.4:-14185486 None:intergenic
AATGAGAACAAGAATACGAA+TGG 0.596334 7.4:+14186914 MS.gene048625:CDS
TTGGCATAAACTGGGTCCAA+GGG 0.596569 7.4:-14192067 None:intergenic
GGATCAAATATATGACAGAT+GGG 0.600655 7.4:+14186417 MS.gene048625:CDS
AAGCGAGTTGCCGAAGAATG+TGG 0.604736 7.4:+14186329 MS.gene048625:CDS
GCTGCTTCACAGCTGCATTG+AGG 0.605522 7.4:-14192853 None:intergenic
GATACCTTGCGAAACCCAGA+TGG 0.606659 7.4:-14187626 None:intergenic
AGCAGCCAGATGGAACCTAC+AGG 0.608253 7.4:+14192870 MS.gene048625:CDS
TTTGGCATAAACTGGGTCCA+AGG 0.609632 7.4:-14192068 None:intergenic
CACGAGCATGTCTAAGAGAA+CGG 0.611248 7.4:-14192633 None:intergenic
TTCCGCAGAAAGCATTGCAA+CGG 0.612393 7.4:-14192140 None:intergenic
AGAAATACTAGTATATCACC+AGG 0.613029 7.4:-14187458 None:intergenic
AGTACCTACATCCAAGTCCC+AGG 0.615133 7.4:+14189006 MS.gene048625:CDS
GTACATCAAGATGAGGCCCC+TGG 0.615632 7.4:+14187440 MS.gene048625:CDS
TAAGAGCTTCGCAAGTCCAG+AGG 0.617178 7.4:+14192480 MS.gene048625:CDS
AGCCGTTGCAATGCTTTCTG+CGG 0.618592 7.4:+14192138 MS.gene048625:CDS
ATTCTACTTCCAACTCAACA+AGG 0.634787 7.4:+14186396 MS.gene048625:CDS
TGTCAACTATCTGATCCAGT+TGG 0.635262 7.4:+14192025 MS.gene048625:CDS
AATGCCTGGGACTTGGATGT+AGG 0.635279 7.4:-14189010 None:intergenic
GTTATAGGTACATCAAGATG+AGG 0.635310 7.4:+14187433 MS.gene048625:intron
TAAGAGCTTTGGCATAAACT+GGG 0.636597 7.4:-14192075 None:intergenic
TCCGCACGAGAAGTGCGAAG+AGG 0.638157 7.4:+14192177 MS.gene048625:CDS
TGTCAACTCTGGTTAAATCA+CGG 0.646694 7.4:-14193235 None:intergenic
TTCCGGAACAATACAGACGA+AGG 0.647037 7.4:-14193158 None:intergenic
ACACATTAATCAGAGTGATG+TGG 0.651588 7.4:-14192507 None:intergenic
TCAAGGGAGACAGTTTCCTG+TGG 0.671336 7.4:+14187090 MS.gene048625:CDS
GAAATACTAGTATATCACCA+GGG 0.680918 7.4:-14187457 None:intergenic
TTCTGCATAGCGTAAAACTG+AGG 0.692959 7.4:-14193281 None:intergenic
AAATACTAGTATATCACCAG+GGG 0.695525 7.4:-14187456 None:intergenic
AGCCCATCAAGACATCAGTG+TGG 0.744619 7.4:-14186832 None:intergenic
ACGTGTTGCTGCTATCACTG+TGG 0.765431 7.4:+14186304 MS.gene048625:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.4 gene 14185386 14193307 14185386 ID=MS.gene048625
chr7.4 mRNA 14185386 14193307 14185386 ID=MS.gene048625.t1;Parent=MS.gene048625
chr7.4 exon 14185386 14185495 14185386 ID=MS.gene048625.t1.exon1;Parent=MS.gene048625.t1
chr7.4 CDS 14185386 14185495 14185386 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14185636 14185682 14185636 ID=MS.gene048625.t1.exon2;Parent=MS.gene048625.t1
chr7.4 CDS 14185636 14185682 14185636 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14185770 14185850 14185770 ID=MS.gene048625.t1.exon3;Parent=MS.gene048625.t1
chr7.4 CDS 14185770 14185850 14185770 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14186234 14186450 14186234 ID=MS.gene048625.t1.exon4;Parent=MS.gene048625.t1
chr7.4 CDS 14186234 14186450 14186234 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14186760 14187177 14186760 ID=MS.gene048625.t1.exon5;Parent=MS.gene048625.t1
chr7.4 CDS 14186760 14187177 14186760 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14187440 14187643 14187440 ID=MS.gene048625.t1.exon6;Parent=MS.gene048625.t1
chr7.4 CDS 14187440 14187643 14187440 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14188881 14189041 14188881 ID=MS.gene048625.t1.exon7;Parent=MS.gene048625.t1
chr7.4 CDS 14188881 14189041 14188881 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14189833 14190018 14189833 ID=MS.gene048625.t1.exon8;Parent=MS.gene048625.t1
chr7.4 CDS 14189833 14190018 14189833 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14191967 14192198 14191967 ID=MS.gene048625.t1.exon9;Parent=MS.gene048625.t1
chr7.4 CDS 14191967 14192198 14191967 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14192466 14192665 14192466 ID=MS.gene048625.t1.exon10;Parent=MS.gene048625.t1
chr7.4 CDS 14192466 14192665 14192466 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14192757 14192891 14192757 ID=MS.gene048625.t1.exon11;Parent=MS.gene048625.t1
chr7.4 CDS 14192757 14192891 14192757 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
chr7.4 exon 14193124 14193307 14193124 ID=MS.gene048625.t1.exon12;Parent=MS.gene048625.t1
chr7.4 CDS 14193124 14193307 14193124 ID=cds.MS.gene048625.t1;Parent=MS.gene048625.t1
Gene Sequence

>MS.gene048625

ATGGTGTTTGGTCAGAGAGATCGTAACAAGCCTTTTGTGGCTCGTCGAAATTACCAACCCAATGCTAATGCCAACGCCAATCGCAATAACCAAACCCATTCCTCTCCCTGGTATTACTTTTCCTAATTCATTTCAATTTTTACTCCTTTTCAACTAAAATTATTCATGCTCAAAAGGGTAGCAGAAAAAGACCAAATTTTGAATTGGGTTTTAGTTTTTTTTTCTTTCTAATTTGTTAATTTTATTACAGGAGACAGAAAATTATAGAGCAGAGAAAGTCACTTCCTATTGCTTCGGGTATTGAAATTTGTTTGATTTTTGTGTTTTGAATGTTCTTACTTTCATATTTATCATGTGTGACCGTTGGCATTGTTTTTATTGTAGTTGAGAAGAGATTAATTGAAGAGGTTCGGAAAAATGATATTTTGATTATTGTTGGTGAAACTGGAAGTGGAAAAAGTACACGTAGGTTACATCTTATCCTATTAATCAATATGTTTATGATTATATCCTTTATAATTTAGGGTATATTTGGATTTACTTATCTGATTATTTACTCGCATAAGCACTTGTGAGAATGTTTGAGAGTTTTTATGAAAACAACAAATAACATGTTCATAAGCTGTTAGATTTGAAAAGAGCTTATAGCTTATATGAAAACAATTTGGCTTTGTTTTATCTTTTGTTTAAAAATAATTTATGTTGTATGATAAGCGCTTATGTTGTACTTGCATAATTTAGCTGTTTATTAAAACATGCCTTAGGTTTTTAGGAAAAGAAACGAAAAAAGGAATTTTAGAGATAGCGGGTTTTCACTGTGTTTATGTTTATTGCATTTTACCGTGTAGAGATTCCACAATTTTTGTTTCATGCTGGATTTTGCCATGACGGAAAAGTTGTTGGGATAACTCAACCTAGACGTGTTGCTGCTATCACTGTGGCCAAGCGAGTTGCCGAAGAATGTGGTTGTGAGTTGGGCCAAAAGGTTGGGTACTCTGTTAGATTTGATGATTCTACTTCCAACTCAACAAGGATCAAATATATGACAGATGGGTTACTTCTGAGGTACAACTTTTTTGTAGCTTTGATTTCTGGGAGTGTTTCCTATCAAGACATGATACTGTGATTTGTTTTTTCTTTTCATTCTTGATGTCAAACTATAAAAATACCATATCACTTTTTTTGAATCAATTGTATTCAACGACGGGTGAGTAACATCCCCTTCCCCGTCGTTGATGCAGGTTTGAGATAGTTACATGATTTGAAGTAGTTAAGGAGGTTTCAGATTAAATATTTTGTTTTTCATGGTTTGTATTTGTAAGTACTGTTTTTCTTATGGTAAAGTTAATAAGCATTTTTTACTTGTGTTTACAGGGAAGCATTGTTGGATCCTTATCTTTCCAAGTATTCTGTTATAATTGTCGATGAAGCACACGAGAGAACTGTCCACACTGATGTCTTGATGGGCTTGCTAAAAAATGTGCAACATGCTCGGTCAAATTCTATCAAAGATGGTTGGAACCTTGATAATGAGAACAAGAATACGAATGGTTTCATGTTGGATAAAGATAATGGCCAGAATGACAGTTTTCTTAGAAAAGACCACCGCGAAAAGAGTTCTCCGTTGAAGTTAATCATCATGTCTGCAAGTCTTGATGCTCGTACTTTCTCTGAGTACTTTGGTGGTGCCAAAGCTGTTCACGTTCAAGGGAGACAGTTTCCTGTGGATATATTTTATACTCGTCATCCAGAAACAGATTATGTAGATGCTGCCTTGATAACAATTTTCCAGGTCATTGGTTTAATCAGTACATGTAATAATTTAATAACGTGGTTTAACTTATTTTTTTGTAAGAGACCTTAGATCCTAATAATATTTTTTTGGGACAGTGGTTTTTTGGTATGCATTTATGGTACATGTTTGTGCACTGCCTCAGTCATTCAGACATACTGCTACACACACACACACACACAAGATTATAACATTGTGGTGATGGCTGAGAAGTTAAGTTCATATAATTGTCACTTAAAGTCATGTGTCTTTGATGTTATAGGTACATCAAGATGAGGCCCCTGGTGATATACTAGTATTTCTGACTGGGCAAGAAGAGATTGATGCTGTTGAACGGCTTATCAAGGAGCGACTTACAAAGTTACCTCAAGAAAATCAGAAGCTTCAAGTTGTGCCTATATATGCAGCTCTTCCATCTGAGCAGCAAATGCGAGCCTTTGATCCGGCTCCATCTGGGTTTCGCAAGGTATCCTGTAGCTATGCATATTATCCTTTGTTAAATTATCGTATTACTTCTGTGTAGAATTCAATAGAAAAATCAGGAAAATAAGTTTAATTTAACCAATCATTATTTTTTTATTTAATTTAAACGGTTGACATTTCCATCTAGGTTTTTATTTATTCTCTTGAAGCACTTTGTTTCCCATGACCATGTTAATTTATAAAGTAAATGCTGGTTCCTGATTGCCGCATTTAGTGCCTCAGCGTGTTTAGAACATATGCTCGTGCTTTGATATTTCATATTTTGTTATATGGAAGTTTCATCATTGGAAGTTCTCTTAGACACATATGACTTATATAATGATCATCTTCTGTTAGAAGTTAGAACTGGTATCGAAAGTAGAAAAAGTAAGATCGAAAAATGAAAATTGCAAAGGAGTGGTTCTTGAATTGATAACAATATGCACCCTGGCCAGTATATCATAGTGGTGGTGGATACACCAGCCTCGGGTTTCAAGTACCTGCAACTTCCAAATTCTACAATCCCCACAAACTCACCACTTTCTAGTTCCACCTGTATTTATAAGTTATAACTCCCTCTTCCTGTCTCTCCTTGATTAGTTATTCAGATGAAATGTATTTCCTATACTGTCCCCTGCTCTTCCTCATATCACTATAAATTACTAGAAGTTTCCTAACATCTTCCATTTACATTCATGATTTGTTACCTATAGTCTCTATCATTTGACAGTGTTGCCATTGTGGTCCAATTACTCTGATGTCAAATTTAGAACTAATTTTTTTTTCTTCCAATGTCATTATTTGCTTTGTTGCTTCTAACAGTGGATCACTTTGGTTGTAAAGTACTATTTGTATCGTTTTTATACCGTCATACTTACCGAAATGCACCTACCAGTATCTATGTATACCGACCTATTATTTTCCTTTACTTATAACCTTCATCGTGTAATATGTTACCATCACCTATACTCCTTTCGGAACTATGCCTTCTGAACTGGCAACAATCCCTATAAATAGTACATGGATATACAAAACAGTGTCTGCTAGGAGTAGGACCATATTTTTTTCCCATAGTAGAGTGTCACATTTTTAGTTTGAGCCTGTGAATAACAATAACACCAATCAAGCCTTTAATGTGAGCTAATTTATATTCAAAGTAACCTCTGATATTTAATATTTATCATAGCAGGTAATCGCAATTCTTAAATTGTATTCAACTTGGTACTTTGTTGTTTTGTTAGGTGGTATTGGCAACTAATATTGCTGAGACATCAATTACAATTCCTGGAATCAAGTATGTAATTGATCCTGGACTTGTAAAAGCGCGTTCCTATGATCCTGGAAAAGGCATGGAATCTTTGATTGTAGTACCTACATCCAAGTCCCAGGCATTGCAAAGAAGGTATGGTGCTGGATTATGTTTCAATCTGTTTAATATTTGATTCTGTTTGATGTTTAATTGCTGCTGCAATTGTTGGTTGAATTTAGTTAATGTAATGTAAATGAAGTAAGTTTGGTTCAGTTTAACTGAGGATGACTTCCTTTCTCATTTCAAGTTCAGTTTATTAATGGATACCTTTGATTTTCCTCAAAAACGTGTATTTTCTTAACAAAGGTTTTTGAAATGTGAATCTAGCATCATTATTTCTAGCACTTGTTTTTTGTTAAACATGGCGAGGCATAACAACAATTAAGCAAAACCTTATGGTTGTGGTCGGCTATGAGTCAAATGATGTCATAATTCAAATGACATCATAATCAAAACCATTGATTTCCCTCAAAAACATGTATTTTCTTAACAAAGACTTTTGGAACGTGAATCTAGCATCATTGTTTCTAGAACTTGTTTTTTATTACACATACCGAGGCATTACAACAACAATAATAAAACCTTATTCCACTAGTTGGGGTCGGCTATATGAGTCAAATGACGTCGTAGTTCCCTATCATGTGATAAGTTCAGAAATAGATTCTCTACTTCTAAATCTCTCTTGATGGTTTGGCCTATAGTTTTTCATGGTCTCACTCCACCTTTAACCATTGGACTGTCCTCTATCTAGCAAACTTTCCTTGACAGTGTCGTGTGTGTGCACTCGTCCATCTTAACCTTCTCATTATGTGACATAGGTAATATATTTAATTTGATTTTGCTATAAGAGTAGTGCAAATTGATTATCTATATTTTGGTAACAGTGGGCGTGCAGGGCGTGAAGGACCTGGAAAATGCTTTCGGCTATATCCAGAAAATGAATTTGAGAAACTTGAGGATTCTACAATGCCGGAAATTAAGCGTTGCAACCTTTCTAATGTCATTTTGCAGCTTAAGGCTTTGGGTGTTGATGACATATTAGGGTTCGATTTCATTGAGAAACCTTCAAGGTGAGAACCTTGTCAAAATTTTGGTTTGACAACTTCACTACCGATGCATATTTGTGGATAAGTTCTGATTGCTTATGTTTTTAAGGCTAAATTACTATGTAGATTTTAAAGTTTCACTTTGTTTTTAAATAGGTTCTTACACTTCAAAAAAATTAGTGTCTGTGATTCTAAAGATTTTTGTACGAGGGCCAACTACCAAGTACTATTAAGACAATGCAAGCTTTTGCTTAATTTGGAGACCCATTTGAAAGCATAGATAAGCTGAAGAGTTTGAGAGATATCACAGTTAAAAAGGCCCCTATACACTTAATCTTAATTTTCAAATAGGTCCTTATAAGCTATAATTTTGCTAAAGTTAACAGCCATTAGGGAGTAGGGATCTTATAAATTTGTCTAAACTATAAGAATCTGTTTGAAAATTCATGAAACTATAGGGATCTGCAGACTAATGAAGACTATTTAAAATAAAATATGCATGTCCTGAAAGCTTTTTTTTTTCTAACTTCTGCTTATCTTTTAAAAGGATTTTAAGTCACTTGAAAAGATGCTATTATCTCTCTACTAAGATGATTCCTTCAACCTATATAGGTCCTTTTGATTAACCTTTGATTTCTTTTTGTTGTAATGTGATCTAAACTGGAGATAAAAAATCCATTTGAATATTAGGTGCTTTAATTCAATTTTTCTTGGTCATGAAAAATAAAATTATTGAGGATTGACTATATTGTATTCATATACCGAAAGAATAAGAATGACATGACATTTGTATTGCAAATAACCAAAAATCTATAAAAGTGTCATATATATTATCAGGGGCGGATCTACATGTAGTTGAGGGTGTGCACCTGCACACCCTGAACTTTCAACATTTACATGCATAACGCTGTATTTTTTACACTTTGCATACCCTGAAAATTTCACTTTGCACCCGCGGTCCACTCTTTTCTCGCAAATAAGTAAGAAACTGAAGAGAAGAAAAGAAAACGAAGTGAAGAAGAGGTGCAGCACGCTAGAGAAAGAAGAGTGCAGAATTAGTTTAGTTTGAGATCTGTAGTAATGTGGAACCAGAGAAGAAGATGTAGTGCGGCAGCATATTCCTTGCAACAAATAATTAGGGTTATGTAGTATGTTATATTATTACGGGCTTGGGCCTTCCAAATGTTATTTTTGTCTAATGACACTAAGAGGGAAATTGCTCTTTAGCACCATCTAATTGCTCTATTTTGGTTTAACCATATTTAAAAAATTCTTCAAACGGTAGTTAATTGTTGAAAATATTTTTATTATTTCGGGTTGTCTGAGAGCAAATCATGGGTACCTAAAGAACAATTTTCCACTAAGAGTTGTGTTACATGAGCATGTTTTTGGTTCAAAATAATGCTTTTGGATAGTTTGGAACAACAGAAAAATTAAAATTTGGAAAAATAAATTATTAGCAAAATAAAAAAGTATATTAGAGGTTGAATTTTTCGAAGTTAAATTTTTTGCACCCCCTGACCTAGGGTCCTGGATACGCCACTGTATATTATTAATGCAAAAGTACTTTTAGGAATACTTGTATACTGATGAAGAAACTGAGAAGAGTACATAGACAACACCTAATAAAACCTTGCTTGGACCAAGATCTTGCACCTTAAAATCAATCTATAAAGATAAATTTCTATTACAAAATATTGAAAGTGGGCTTTAGTGAATTTTTTTGGACTGAATGGAGAAAATAAATAAAGGATGTAGATAATCTATTCCTTTGCATCAGTTATTAATAGTGAGTTCAAATATTTGAAACTTCTATTGATTCAGTTTCTTTAATGTCAGTTGTCTCGTCCATTGTACTCCAGAACTAAATTAACATTTTTTTAGAGTTATTCGTTTCTTTCTTTATTTTCGAAAAGTAATATCACTGAGCAACATATTACTCAAAGCTTGTACCTGTCACATGTTTTAACAGGACAGCTATTATAAAATCATTGGAGCAGCTATTTTTGTTAGGTGCTTTGACAGATGACTGTCAACTATCTGATCCAGTTGGACGTCAAATGGCCCGACTTCCCTTGGACCCAGTTTATGCCAAAGCTCTTATTTTAGCAAGTCAGTTTAACTGCTTGGAAGAGATGTTGATAGCCGTTGCAATGCTTTCTGCGGAATCCATATTTTATTTTCCGCACGAGAAGTGCGAAGAGGTTTGAAGAGTGAATTCGGATTGTAAACCATTCCTGTCATATTCTATTATTCTATTTCGGTGAAATGTAAATACGAGCATAAATCATTGATTATGTGCTATGCCACTCTATTGGTATATTCACCGTTTAACAAAATATTTGAAAAATATCATACGGATGGTTTAACTATATGCGATTGGTAATTGCCGTAGGAAGATTCTAGTCTTTATTTGACGTCAACTTGTCTTCTCTACACTAACTTATTGTGCTTCTCTGATTTATGGCCAGGCAAGAACTGCAGCTAAGAGCTTCGCAAGTCCAGAGGGAGACCACATCACTCTGATTAATGTGTATAGAGCAGCTACTGATTCATTGGAGAAGAGAACTATCGAAACTAATAAAACAAAAAATGAAAAGGATTTTAGAAAATGGTGCAAAGAAAATTTTATAAATAGCCGTTCTCTTAGACATGCTCGTGACATTCACAGGTTGTTTTATTGTGTTTAAACTTTACTTCAAGGCATCTATAATATCCTTGAGACATTTTATGCATTCTAATTTCTGAAATGCCTTTTTCAGGCAGATTCAAGGACATGTTCAACAAATGGGTCTGAAACTTGCTTCTTGTGGAGAAGATATGCTTCAATTTCGCAGATGCCTTGTTGCTTCCTTTTTCCTCAATGCAGCTGTGAAGCAGCCAGATGGAACCTACAGGTATCCATTTTAACTTCAAAATTCTTGGGCAGTCCCGCTAGGGTGGCTTGCCATCTAATTGGAATCATTACATGAAAATATCGTTTAGTTTCGGGATTTTTCATTGGTAATTATGATTCGCTAATTGGAATCCTGACCTTACATGCATATACACTCTTCAAATATTTTGCTTTTTTGTTGTTACTTATACTTTTATGATAAAAAAGACTCTTACTGCTTCTTTACACAACAGGGCTTTGGCAAGTGGTCAGGTGGTGCAGATCCATCCTTCGTCTGTATTGTTCCGGAAGAAACCAGAGTGCATAATATTTAACGAACTGATTCAAACTAATAACAAGTATGTCCGTGATTTAACCAGAGTTGACAACCTATGGTTAACTGAACTGGCTCCTCAGTTTTACGCTATGCAGAATTGA
Protein sequence

>MS.gene048625.t1

MVFGQRDRNKPFVARRNYQPNANANANRNNQTHSSPWRQKIIEQRKSLPIASVEKRLIEEVRKNDILIIVGETGSGKSTQIPQFLFHAGFCHDGKVVGITQPRRVAAITVAKRVAEECGCELGQKVGYSVRFDDSTSNSTRIKYMTDGLLLREALLDPYLSKYSVIIVDEAHERTVHTDVLMGLLKNVQHARSNSIKDGWNLDNENKNTNGFMLDKDNGQNDSFLRKDHREKSSPLKLIIMSASLDARTFSEYFGGAKAVHVQGRQFPVDIFYTRHPETDYVDAALITIFQVHQDEAPGDILVFLTGQEEIDAVERLIKERLTKLPQENQKLQVVPIYAALPSEQQMRAFDPAPSGFRKVVLATNIAETSITIPGIKYVIDPGLVKARSYDPGKGMESLIVVPTSKSQALQRSGRAGREGPGKCFRLYPENEFEKLEDSTMPEIKRCNLSNVILQLKALGVDDILGFDFIEKPSRTAIIKSLEQLFLLGALTDDCQLSDPVGRQMARLPLDPVYAKALILASQFNCLEEMLIAVAMLSAESIFYFPHEKCEEARTAAKSFASPEGDHITLINVYRAATDSLEKRTIETNKTKNEKDFRKWCKENFINSRSLRHARDIHRQIQGHVQQMGLKLASCGEDMLQFRRCLVASFFLNAAVKQPDGTYRALASGQVVQIHPSSVLFRKKPECIIFNELIQTNNKYVRDLTRVDNLWLTELAPQFYAMQN