Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049131.t1 | XP_003630811.2 | 99.3 | 141 | 1 | 0 | 1 | 141 | 1 | 141 | 3.70E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049131.t1 | Q54UU1 | 55.2 | 125 | 55 | 1 | 4 | 127 | 3 | 127 | 2.2e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049131.t1 | G7L8Y7 | 99.3 | 141 | 1 | 0 | 1 | 141 | 73 | 213 | 2.7e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96826 | MS.gene049131 | PPI |
| MS.gene59549 | MS.gene049131 | PPI |
| MS.gene031550 | MS.gene049131 | PPI |
| MS.gene049131 | MS.gene87621 | PPI |
| MS.gene006753 | MS.gene049131 | PPI |
| MS.gene85984 | MS.gene049131 | PPI |
| MS.gene068341 | MS.gene049131 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049131.t1 | MTR_8g103680 | 99.291 | 141 | 1 | 0 | 1 | 141 | 73 | 213 | 8.99e-105 | 298 |
| MS.gene049131.t1 | MTR_5g075330 | 91.489 | 141 | 12 | 0 | 1 | 141 | 1 | 141 | 7.99e-99 | 280 |
| MS.gene049131.t1 | MTR_5g075330 | 91.489 | 141 | 12 | 0 | 1 | 141 | 34 | 174 | 1.10e-98 | 281 |
| MS.gene049131.t1 | MTR_8g095450 | 29.787 | 141 | 84 | 3 | 7 | 132 | 28 | 168 | 1.42e-14 | 67.0 |
| MS.gene049131.t1 | MTR_4g048530 | 29.078 | 141 | 85 | 3 | 7 | 132 | 26 | 166 | 1.92e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049131.t1 | AT5G02280 | 87.943 | 141 | 17 | 0 | 1 | 141 | 1 | 141 | 1.92e-94 | 269 |
| MS.gene049131.t1 | AT1G51160 | 28.369 | 141 | 86 | 3 | 7 | 132 | 26 | 166 | 7.78e-14 | 65.1 |
| MS.gene049131.t1 | AT1G51160 | 28.369 | 141 | 86 | 3 | 7 | 132 | 26 | 166 | 7.78e-14 | 65.1 |
| MS.gene049131.t1 | AT1G51160 | 28.369 | 141 | 86 | 3 | 7 | 132 | 26 | 166 | 7.78e-14 | 65.1 |
Find 32 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGTTGTCACCTGTTTC+TGG | 0.050534 | 4.1:+2611291 | MS.gene049131:CDS |
| ATTTAGGATTATGGGTCTTC+TGG | 0.243224 | 4.1:+2611204 | MS.gene049131:intron |
| CTTTCCATTTGTTGAGTTCC+AGG | 0.279445 | 4.1:-2611905 | None:intergenic |
| TTTCTGATATTTAGGATTAT+GGG | 0.304809 | 4.1:+2611196 | MS.gene049131:intron |
| GAAGGACCGTGTTGCATTGC+TGG | 0.349606 | 4.1:+2612045 | MS.gene049131:CDS |
| AGGCATCTCCATCTCATAGA+AGG | 0.407862 | 4.1:-2611978 | None:intergenic |
| TGGTGGATTGATCTACTATA+AGG | 0.415123 | 4.1:+2610539 | MS.gene049131:CDS |
| GCTTACGGGTGGCTAGCTTA+TGG | 0.427608 | 4.1:+2611247 | MS.gene049131:CDS |
| ATTCCTGCGCAACCAGAAAC+AGG | 0.450165 | 4.1:-2611303 | None:intergenic |
| AAATAGTACCTGTCAGTGAC+TGG | 0.450546 | 4.1:-2611365 | None:intergenic |
| CTTTACATCATCAATAAGTC+TGG | 0.473288 | 4.1:+2610519 | MS.gene049131:CDS |
| TGAACCTGGAACTCAACAAA+TGG | 0.475337 | 4.1:+2611901 | MS.gene049131:CDS |
| TCAAAGAGCTCACATCGTAT+AGG | 0.480843 | 4.1:-2611998 | None:intergenic |
| GGACACCAATGATAGCTTAC+GGG | 0.481121 | 4.1:+2611233 | MS.gene049131:CDS |
| GGCATCTCCATCTCATAGAA+GGG | 0.506472 | 4.1:-2611977 | None:intergenic |
| AGCCACCCGTAAGCTATCAT+TGG | 0.510288 | 4.1:-2611238 | None:intergenic |
| TGGACACCAATGATAGCTTA+CGG | 0.512682 | 4.1:+2611232 | MS.gene049131:CDS |
| CATTGCTTCCAGTCACTGAC+AGG | 0.515176 | 4.1:+2611357 | MS.gene049131:CDS |
| GAAGAACCCCTTCTATGAGA+TGG | 0.515244 | 4.1:+2611970 | MS.gene049131:CDS |
| TCACCTGTTTCTGGTTGCGC+AGG | 0.516213 | 4.1:+2611300 | MS.gene049131:CDS |
| CTTTGATATCAACTTAGCTC+AGG | 0.519060 | 4.1:+2612015 | MS.gene049131:CDS |
| AAGGACCGTGTTGCATTGCT+GGG | 0.527169 | 4.1:+2612046 | MS.gene049131:CDS |
| GAAGATCAAATGTGTCAGCT+TGG | 0.530412 | 4.1:-2611335 | None:intergenic |
| CTTAGCTCAGGCAGTGCAGA+AGG | 0.554879 | 4.1:+2612027 | MS.gene049131:CDS |
| TACATCATCAATAAGTCTGG+TGG | 0.561761 | 4.1:+2610522 | MS.gene049131:CDS |
| TTATGGGTCTTCTGGACGAA+TGG | 0.565210 | 4.1:+2611212 | MS.gene049131:CDS |
| AGGTGACAACTGCTGAGAGA+TGG | 0.604194 | 4.1:-2611283 | None:intergenic |
| AAAGGCTTTAGGAATGGCAG+CGG | 0.636991 | 4.1:+2610488 | None:intergenic |
| GCATCTCCATCTCATAGAAG+GGG | 0.643808 | 4.1:-2611976 | None:intergenic |
| CACCAATGATAGCTTACGGG+TGG | 0.665422 | 4.1:+2611236 | MS.gene049131:CDS |
| ATTCGTATATGAATTGTACA+CGG | 0.693192 | 4.1:+2611937 | MS.gene049131:CDS |
| ATCGACCCAGCAATGCAACA+CGG | 0.698434 | 4.1:-2612051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAATACTAAAAAATTT+AGG | + | chr4.1:2610795-2610814 | MS.gene049131:intron | 10.0% |
| !! | GAAAGTTTAGTAATAATTAT+TGG | + | chr4.1:2611583-2611602 | MS.gene049131:intron | 15.0% |
| !!! | TGTTTAATTTTCTGATATTT+AGG | + | chr4.1:2611188-2611207 | MS.gene049131:intron | 15.0% |
| !!! | TTATTTTTATTGTAAGCTTA+AGG | - | chr4.1:2610906-2610925 | None:intergenic | 15.0% |
| !!! | ATTTAAAATCTACTAGTACT+AGG | - | chr4.1:2611813-2611832 | None:intergenic | 20.0% |
| !!! | TTGAGTAAAACATTTTTCAT+AGG | - | chr4.1:2610632-2610651 | None:intergenic | 20.0% |
| !!! | TTTCTGATATTTAGGATTAT+GGG | + | chr4.1:2611196-2611215 | MS.gene049131:intron | 20.0% |
| !!! | TTTTCTGATATTTAGGATTA+TGG | + | chr4.1:2611195-2611214 | MS.gene049131:intron | 20.0% |
| ! | AAAAACTTTCTGAGTTAGAT+GGG | - | chr4.1:2611095-2611114 | None:intergenic | 25.0% |
| ! | ATTCGTATATGAATTGTACA+CGG | + | chr4.1:2611937-2611956 | MS.gene049131:CDS | 25.0% |
| ! | CTTAACATAATGATGAGTTA+TGG | - | chr4.1:2611392-2611411 | None:intergenic | 25.0% |
| ! | TAAAAACTTTCTGAGTTAGA+TGG | - | chr4.1:2611096-2611115 | None:intergenic | 25.0% |
| ! | TGTGAATAAAAAGCAAACAT+TGG | - | chr4.1:2611493-2611512 | None:intergenic | 25.0% |
| !! | AAAGTTTTTACTACTGTTGT+TGG | + | chr4.1:2611106-2611125 | MS.gene049131:intron | 25.0% |
| !! | CAAACATAATTTGTAACTAC+TGG | - | chr4.1:2611680-2611699 | None:intergenic | 25.0% |
| !!! | TGTTTGCTTTTTATTCACAT+TGG | + | chr4.1:2611494-2611513 | MS.gene049131:intron | 25.0% |
| AAAACTTTCTGAGTTAGATG+GGG | - | chr4.1:2611094-2611113 | None:intergenic | 30.0% | |
| AAGAATGAATGCCAAAAATC+TGG | + | chr4.1:2611439-2611458 | MS.gene049131:intron | 30.0% | |
| CTTTACATCATCAATAAGTC+TGG | + | chr4.1:2610519-2610538 | MS.gene049131:CDS | 30.0% | |
| TACATACAGGTAAAATTCCA+AGG | - | chr4.1:2611626-2611645 | None:intergenic | 30.0% | |
| TACTAGTACTAGGTTATAGA+TGG | - | chr4.1:2611803-2611822 | None:intergenic | 30.0% | |
| ! | AATAAGTTGGGTTGTTAATC+AGG | + | chr4.1:2610673-2610692 | MS.gene049131:intron | 30.0% |
| ! | AGTGATATGTACCAGATTTT+TGG | - | chr4.1:2611453-2611472 | None:intergenic | 30.0% |
| ! | ATAATTTGTAACTACTGGTG+CGG | - | chr4.1:2611675-2611694 | None:intergenic | 30.0% |
| !!! | TGATTTTTGGTTTTAGGAGA+TGG | + | chr4.1:2610838-2610857 | MS.gene049131:intron | 30.0% |
| AAGAAAAAACTTCAAGCCCA+CGG | - | chr4.1:2610600-2610619 | None:intergenic | 35.0% | |
| AATTAATTCTTCTCTGCCAC+AGG | + | chr4.1:2611857-2611876 | MS.gene049131:intron | 35.0% | |
| ACCCAACTTATTCATCAAAC+AGG | - | chr4.1:2610665-2610684 | None:intergenic | 35.0% | |
| ATCTGGTACATATCACTGAT+TGG | + | chr4.1:2611456-2611475 | MS.gene049131:intron | 35.0% | |
| ATTAATTCTTCTCTGCCACA+GGG | + | chr4.1:2611858-2611877 | MS.gene049131:intron | 35.0% | |
| CCCAACTTATTCATCAAACA+GGG | - | chr4.1:2610664-2610683 | None:intergenic | 35.0% | |
| CCCTGTTTGATGAATAAGTT+GGG | + | chr4.1:2610661-2610680 | MS.gene049131:intron | 35.0% | |
| CGTAGATTTGTCACCTTAAT+TGG | + | chr4.1:2611709-2611728 | MS.gene049131:intron | 35.0% | |
| CTTTGATATCAACTTAGCTC+AGG | + | chr4.1:2612015-2612034 | MS.gene049131:CDS | 35.0% | |
| TACATCATCAATAAGTCTGG+TGG | + | chr4.1:2610522-2610541 | MS.gene049131:CDS | 35.0% | |
| TGTAATGCCTTGGTCATTTA+AGG | + | chr4.1:2611642-2611661 | MS.gene049131:intron | 35.0% | |
| TTCACATTGGTAAGTAACCT+TGG | + | chr4.1:2611507-2611526 | MS.gene049131:intron | 35.0% | |
| TTTACCTGTATGTAATGCCT+TGG | + | chr4.1:2611632-2611651 | MS.gene049131:intron | 35.0% | |
| TTTGTCAAGGCTATTGATCA+AGG | - | chr4.1:2611035-2611054 | None:intergenic | 35.0% | |
| ! | ATAGCCTTGACAAATCACAA+TGG | + | chr4.1:2611041-2611060 | MS.gene049131:intron | 35.0% |
| ! | ATTTAGGATTATGGGTCTTC+TGG | + | chr4.1:2611204-2611223 | MS.gene049131:intron | 35.0% |
| !! | TAACATAGTCTTATGTGCCT+TGG | + | chr4.1:2611606-2611625 | MS.gene049131:intron | 35.0% |
| !! | TGGTGGATTGATCTACTATA+AGG | + | chr4.1:2610539-2610558 | MS.gene049131:CDS | 35.0% |
| !!! | CGTCGTTGATTTTTGGTTTT+AGG | + | chr4.1:2610832-2610851 | MS.gene049131:intron | 35.0% |
| !!! | CTATTCACGTCGTTGATTTT+TGG | + | chr4.1:2610825-2610844 | MS.gene049131:intron | 35.0% |
| AAATAGTACCTGTCAGTGAC+TGG | - | chr4.1:2611368-2611387 | None:intergenic | 40.0% | |
| ACATTGGTAAGTAACCTTGG+AGG | + | chr4.1:2611510-2611529 | MS.gene049131:intron | 40.0% | |
| GAAGATCAAATGTGTCAGCT+TGG | - | chr4.1:2611338-2611357 | None:intergenic | 40.0% | |
| GCCCTGTTTGATGAATAAGT+TGG | + | chr4.1:2610660-2610679 | MS.gene049131:intron | 40.0% | |
| TCAAAGAGCTCACATCGTAT+AGG | - | chr4.1:2612001-2612020 | None:intergenic | 40.0% | |
| TCACGTCGTAGATGGAAATA+AGG | + | chr4.1:2610863-2610882 | MS.gene049131:intron | 40.0% | |
| TGAACCTGGAACTCAACAAA+TGG | + | chr4.1:2611901-2611920 | MS.gene049131:CDS | 40.0% | |
| TGGACACCAATGATAGCTTA+CGG | + | chr4.1:2611232-2611251 | MS.gene049131:CDS | 40.0% | |
| ! | ATGACCAAGGCATTACATAC+AGG | - | chr4.1:2611639-2611658 | None:intergenic | 40.0% |
| ! | CTTTCCATTTGTTGAGTTCC+AGG | - | chr4.1:2611908-2611927 | None:intergenic | 40.0% |
| AAGCATCACAGTGAACGCAA+AGG | - | chr4.1:2611735-2611754 | None:intergenic | 45.0% | |
| AGGCATCTCCATCTCATAGA+AGG | - | chr4.1:2611981-2612000 | None:intergenic | 45.0% | |
| CTTTCTGAGTTAGATGGGGT+TGG | - | chr4.1:2611090-2611109 | None:intergenic | 45.0% | |
| GAAGAACCCCTTCTATGAGA+TGG | + | chr4.1:2611970-2611989 | MS.gene049131:CDS | 45.0% | |
| GCATCTCCATCTCATAGAAG+GGG | - | chr4.1:2611979-2611998 | None:intergenic | 45.0% | |
| GGACACCAATGATAGCTTAC+GGG | + | chr4.1:2611233-2611252 | MS.gene049131:CDS | 45.0% | |
| GGCATCTCCATCTCATAGAA+GGG | - | chr4.1:2611980-2611999 | None:intergenic | 45.0% | |
| GTGAACGCAAAGGCCAATTA+AGG | - | chr4.1:2611725-2611744 | None:intergenic | 45.0% | |
| TTATGGGTCTTCTGGACGAA+TGG | + | chr4.1:2611212-2611231 | MS.gene049131:CDS | 45.0% | |
| ! | AGATGGATTCACGTCGTAGA+TGG | + | chr4.1:2610855-2610874 | MS.gene049131:intron | 45.0% |
| ! | CAAGGCACCTTAAATGACCA+AGG | - | chr4.1:2611652-2611671 | None:intergenic | 45.0% |
| ! | GACGCCATTGTGATTTGTCA+AGG | - | chr4.1:2611048-2611067 | None:intergenic | 45.0% |
| ! | TTTTTCGTGGTCTGTGAACC+TGG | + | chr4.1:2611887-2611906 | MS.gene049131:CDS | 45.0% |
| AAGGACCGTGTTGCATTGCT+GGG | + | chr4.1:2612046-2612065 | MS.gene049131:CDS | 50.0% | |
| ACAGCACAGACTGCACAGTA+CGG | - | chr4.1:2611538-2611557 | None:intergenic | 50.0% | |
| AGCCACCCGTAAGCTATCAT+TGG | - | chr4.1:2611241-2611260 | None:intergenic | 50.0% | |
| AGGTGACAACTGCTGAGAGA+TGG | - | chr4.1:2611286-2611305 | None:intergenic | 50.0% | |
| ATCGACCCAGCAATGCAACA+CGG | - | chr4.1:2612054-2612073 | None:intergenic | 50.0% | |
| ATTCCTGCGCAACCAGAAAC+AGG | - | chr4.1:2611306-2611325 | None:intergenic | 50.0% | |
| CACCAATGATAGCTTACGGG+TGG | + | chr4.1:2611236-2611255 | MS.gene049131:CDS | 50.0% | |
| CACGAAAAACTTCGTCCCTG+TGG | - | chr4.1:2611876-2611895 | None:intergenic | 50.0% | |
| CAGCAGTTGTCACCTGTTTC+TGG | + | chr4.1:2611291-2611310 | MS.gene049131:CDS | 50.0% | |
| CATTGCTTCCAGTCACTGAC+AGG | + | chr4.1:2611357-2611376 | MS.gene049131:CDS | 50.0% | |
| CGGTAGAGACTAGAGAGCAA+TGG | - | chr4.1:2610580-2610599 | None:intergenic | 50.0% | |
| TGCTCTCTAGTCTCTACCGT+GGG | + | chr4.1:2610581-2610600 | MS.gene049131:intron | 50.0% | |
| TTGCTCTCTAGTCTCTACCG+TGG | + | chr4.1:2610580-2610599 | MS.gene049131:intron | 50.0% | |
| TTGTAACTACTGGTGCGGCA+AGG | - | chr4.1:2611670-2611689 | None:intergenic | 50.0% | |
| ! | CACAGGGACGAAGTTTTTCG+TGG | + | chr4.1:2611874-2611893 | MS.gene049131:intron | 50.0% |
| CTTAGCTCAGGCAGTGCAGA+AGG | + | chr4.1:2612027-2612046 | MS.gene049131:CDS | 55.0% | |
| GAAGGACCGTGTTGCATTGC+TGG | + | chr4.1:2612045-2612064 | MS.gene049131:CDS | 55.0% | |
| GTACTGTGCAGTCTGTGCTG+TGG | + | chr4.1:2611537-2611556 | MS.gene049131:intron | 55.0% | |
| !! | GCTTACGGGTGGCTAGCTTA+TGG | + | chr4.1:2611247-2611266 | MS.gene049131:CDS | 55.0% |
| !! | TCACCTGTTTCTGGTTGCGC+AGG | + | chr4.1:2611300-2611319 | MS.gene049131:CDS | 55.0% |
| GTGCAGTCTGTGCTGTGGCT+GGG | + | chr4.1:2611542-2611561 | MS.gene049131:intron | 60.0% | |
| TGCACAGTACGGTGCCTCCA+AGG | - | chr4.1:2611527-2611546 | None:intergenic | 60.0% | |
| TGTGCAGTCTGTGCTGTGGC+TGG | + | chr4.1:2611541-2611560 | MS.gene049131:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 2610501 | 2612075 | 2610501 | ID=MS.gene049131 |
| chr4.1 | mRNA | 2610501 | 2612075 | 2610501 | ID=MS.gene049131.t1;Parent=MS.gene049131 |
| chr4.1 | exon | 2610501 | 2610560 | 2610501 | ID=MS.gene049131.t1.exon1;Parent=MS.gene049131.t1 |
| chr4.1 | CDS | 2610501 | 2610560 | 2610501 | ID=cds.MS.gene049131.t1;Parent=MS.gene049131.t1 |
| chr4.1 | exon | 2611210 | 2611378 | 2611210 | ID=MS.gene049131.t1.exon2;Parent=MS.gene049131.t1 |
| chr4.1 | CDS | 2611210 | 2611378 | 2611210 | ID=cds.MS.gene049131.t1;Parent=MS.gene049131.t1 |
| chr4.1 | exon | 2611879 | 2612075 | 2611879 | ID=MS.gene049131.t1.exon3;Parent=MS.gene049131.t1 |
| chr4.1 | CDS | 2611879 | 2612075 | 2611879 | ID=cds.MS.gene049131.t1;Parent=MS.gene049131.t1 |
| Gene Sequence |
| Protein sequence |
