AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24855


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24855.t1 AT5G12010 66.667 321 106 1 1 320 181 501 6.74e-164 466
MS.gene24855.t1 AT4G29780 65.723 318 108 1 1 317 219 536 1.68e-158 454
MS.gene24855.t1 AT3G55350 29.097 299 177 8 1 276 78 364 3.42e-36 135
MS.gene24855.t1 AT3G63270 29.371 286 174 8 2 267 72 349 1.18e-32 125
MS.gene24855.t1 AT3G19120 25.249 301 185 8 1 279 123 405 1.87e-23 100

Find 78 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTGATGGATGGAATTT+TGG 0.058357 8.2:-6862872 MS.gene24855:CDS
TCTTGGGTTGTTGGAAATTC+TGG 0.088510 8.2:-6862898 MS.gene24855:CDS
CTCTTTGAAACCAGCCTTAA+AGG 0.137684 8.2:+6863324 None:intergenic
TTGCCACCGGTGATCCTTTA+AGG 0.178844 8.2:-6863338 MS.gene24855:CDS
TTGGACTTAGGTGCAATTAT+TGG 0.242423 8.2:+6863117 None:intergenic
GAGGAGTTGCCTGGTGTTCT+TGG 0.249284 8.2:-6862706 MS.gene24855:CDS
AAACAGCTGATTCAAGTTCT+TGG 0.262964 8.2:+6863430 None:intergenic
GTCAAATTCTGATGTGTATA+AGG 0.284658 8.2:+6862847 None:intergenic
CAGGGAATTTGAAAGATTCT+TGG 0.293250 8.2:-6862915 MS.gene24855:CDS
TTGCTAAGGGATGCAATTCC+CGG 0.296879 8.2:-6863393 MS.gene24855:CDS
CTTGGGTTGTTGGAAATTCT+GGG 0.311033 8.2:-6862897 MS.gene24855:CDS
TTATAATGATGATCAGGTTT+TGG 0.315416 8.2:-6862968 MS.gene24855:CDS
AGCGCGTGGCGGTTTGTATC+TGG 0.334097 8.2:-6863365 MS.gene24855:CDS
AAATTCTGGGTTCCCTTTGA+TGG 0.337782 8.2:-6862884 MS.gene24855:CDS
AACCGCCACGCGCTGCCGCC+CGG 0.343618 8.2:+6863374 None:intergenic
TGAATTCTTATTTCAATAAG+AGG 0.366055 8.2:-6863092 MS.gene24855:CDS
TTGAAAGATTCTTGGGTTGT+TGG 0.375328 8.2:-6862907 MS.gene24855:CDS
TACATTGAACCACCAACATT+TGG 0.379629 8.2:+6863153 None:intergenic
ACCGCCACGCGCTGCCGCCC+GGG 0.388713 8.2:+6863375 None:intergenic
GGTTTCAAAGAGGTTCGGTT+TGG 0.398582 8.2:-6863313 MS.gene24855:CDS
GCATACTCTGTTACTGTTCA+AGG 0.400851 8.2:-6863045 MS.gene24855:CDS
TGGTTCTTATAATGATGATC+AGG 0.413194 8.2:-6862974 MS.gene24855:CDS
AGGGAATTTGAAAGATTCTT+GGG 0.414339 8.2:-6862914 MS.gene24855:CDS
GTTTCAAAGAGGTTCGGTTT+GGG 0.421074 8.2:-6863312 MS.gene24855:CDS
CACCGGTGATCCTTTAAGGC+TGG 0.435269 8.2:-6863334 MS.gene24855:CDS
CAGCAAGCACCAAGAACACC+AGG 0.435403 8.2:+6862697 None:intergenic
AAGGTGTGGTTGATACTAAA+GGG 0.444687 8.2:-6863026 MS.gene24855:CDS
TAAGAATTCACATTGGACTT+AGG 0.445097 8.2:+6863105 None:intergenic
TTTGATTAAACAAGAGTTTG+AGG 0.451818 8.2:-6863190 MS.gene24855:CDS
TTATGCCAAAGTTTATTAGT+TGG 0.453461 8.2:-6863233 MS.gene24855:CDS
CAAGGTGTGGTTGATACTAA+AGG 0.464115 8.2:-6863027 MS.gene24855:CDS
TCCCGGGCGGCAGCGCGTGG+CGG 0.468360 8.2:-6863376 MS.gene24855:CDS
GAGGATAGAGGTGAAGATTG+AGG 0.471283 8.2:-6862725 MS.gene24855:CDS
TCAGGCCAACTAATAAACTT+TGG 0.476597 8.2:+6863228 None:intergenic
CTGTTGCTGCTGTTCAAGCT+AGG 0.480095 8.2:-6862573 MS.gene24855:CDS
TCTGGGTTCCCTTTGATGGA+TGG 0.480100 8.2:-6862880 MS.gene24855:CDS
AGGCTGGTTTCAAAGAGGTT+CGG 0.481630 8.2:-6863318 MS.gene24855:CDS
GGTGGTCTTGTTTGCAGAAG+AGG 0.482578 8.2:-6862744 MS.gene24855:CDS
TTGATTAAACAAGAGTTTGA+GGG 0.485947 8.2:-6863189 MS.gene24855:CDS
ACCAAAATTCCATCCATCAA+AGG 0.513056 8.2:+6862871 None:intergenic
GGATATTCAGAAGCTGTGTA+AGG 0.523114 8.2:-6862791 MS.gene24855:CDS
CTCTGTTACTGTTCAAGGTG+TGG 0.523511 8.2:-6863040 MS.gene24855:CDS
CCAAAATTCCATCCATCAAA+GGG 0.523543 8.2:+6862872 None:intergenic
TTGTTTGCAGAAGAGGATAG+AGG 0.524424 8.2:-6862737 MS.gene24855:CDS
TTTGGAATGCCAAATGTTGG+TGG 0.527866 8.2:-6863162 MS.gene24855:CDS
TGAAATGAGAAATGAGAAGA+TGG 0.532464 8.2:-6862656 MS.gene24855:CDS
TATATCCACCTGTCATAAGC+TGG 0.547796 8.2:-6863289 MS.gene24855:CDS
AAAAGTTCAAAATTCCATGC+AGG 0.556616 8.2:+6862631 None:intergenic
TGCTAAGGGATGCAATTCCC+GGG 0.559385 8.2:-6863392 MS.gene24855:CDS
CTCGAGAACCAGCTTATGAC+AGG 0.564326 8.2:+6863281 None:intergenic
AATTCCCGGGCGGCAGCGCG+TGG 0.564819 8.2:-6863379 MS.gene24855:CDS
ATGTATCAGAGAGCTATGAC+AGG 0.567243 8.2:-6862934 MS.gene24855:CDS
GATGAAATGGTTGCTGAGAA+TGG 0.571501 8.2:-6862604 MS.gene24855:CDS
CTTTAAGGCTGGTTTCAAAG+AGG 0.577123 8.2:-6863323 MS.gene24855:CDS
CTGTCATAAGCTGGTTCTCG+AGG 0.578653 8.2:-6863280 MS.gene24855:CDS
ATGAGAAGATGGATCCTGCA+TGG 0.580887 8.2:-6862645 MS.gene24855:CDS
TGATCATCATTATAAGAACC+AGG 0.582500 8.2:+6862976 None:intergenic
TGTTGCTGCTGTTCAAGCTA+GGG 0.584860 8.2:-6862572 MS.gene24855:CDS
GATGCGTTTGCAAAGGTGAA+AGG 0.587098 8.2:-6862769 MS.gene24855:CDS
ATACACATCAGAATTTGACT+TGG 0.591529 8.2:-6862843 MS.gene24855:CDS
AACCAGCCTTAAAGGATCAC+CGG 0.593167 8.2:+6863332 None:intergenic
TTATTGCATCGTGGTCGTGT+TGG 0.605444 8.2:-6862532 MS.gene24855:CDS
ATTGAAATAAGAATTCACAT+TGG 0.606107 8.2:+6863098 None:intergenic
GCACGCGTTTAATGAGAAAG+TGG 0.606857 8.2:-6862815 MS.gene24855:CDS
GTGAAGATTGAGGAGTTGCC+TGG 0.615794 8.2:-6862715 MS.gene24855:CDS
GCTCATGATTTATTGCATCG+TGG 0.641202 8.2:-6862541 MS.gene24855:CDS
TGCAATTATTGGAATATGTG+TGG 0.641263 8.2:+6863128 None:intergenic
TGTATCTGGAGACTTGCCAC+CGG 0.646276 8.2:-6863351 MS.gene24855:CDS
CGTTTGCAAAGGTGAAAGGA+AGG 0.647177 8.2:-6862765 MS.gene24855:CDS
TCAATAAGAGGCAGACTCAG+AGG 0.654570 8.2:-6863080 MS.gene24855:CDS
CGCGTTTAATGAGAAAGTGG+AGG 0.659194 8.2:-6862812 MS.gene24855:CDS
GAGAACCAGCTTATGACAGG+TGG 0.660646 8.2:+6863284 None:intergenic
GTGTAAGGATGCGTTTGCAA+AGG 0.672985 8.2:-6862776 MS.gene24855:CDS
GATCATCATTATAAGAACCA+GGG 0.676277 8.2:+6862977 None:intergenic
TTGCAAAGGTGAAAGGAAGG+TGG 0.690826 8.2:-6862762 MS.gene24855:CDS
CAGCCTTAAAGGATCACCGG+TGG 0.709341 8.2:+6863335 None:intergenic
TGTATCAGAGAGCTATGACA+GGG 0.718997 8.2:-6862933 MS.gene24855:CDS
TAAGGGATGCAATTCCCGGG+CGG 0.733518 8.2:-6863389 MS.gene24855:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAATAAGAATTCACAT+TGG + chr8.2:6862883-6862902 None:intergenic 20.0%
!!! AAATTCATCTTATTTTCATC+AGG + chr8.2:6862771-6862790 None:intergenic 20.0%
!!! TGAATTCTTATTTCAATAAG+AGG - chr8.2:6862886-6862905 MS.gene24855:CDS 20.0%
! AAAGAAAAACACTTTGCTAA+GGG - chr8.2:6862572-6862591 MS.gene24855:CDS 25.0%
! TTATGCCAAAGTTTATTAGT+TGG - chr8.2:6862745-6862764 MS.gene24855:CDS 25.0%
! TTGATTAAACAAGAGTTTGA+GGG - chr8.2:6862789-6862808 MS.gene24855:CDS 25.0%
! TTTGATTAAACAAGAGTTTG+AGG - chr8.2:6862788-6862807 MS.gene24855:CDS 25.0%
!! ATTTTCACTGATGTTTTTCT+TGG - chr8.2:6862975-6862994 MS.gene24855:CDS 25.0%
!! TTATAATGATGATCAGGTTT+TGG - chr8.2:6863010-6863029 MS.gene24855:CDS 25.0%
!!! TGAACTTTTTGATGATGAAA+TGG - chr8.2:6863361-6863380 MS.gene24855:CDS 25.0%
AAAAGTTCAAAATTCCATGC+AGG + chr8.2:6863350-6863369 None:intergenic 30.0%
AGGGAATTTGAAAGATTCTT+GGG - chr8.2:6863064-6863083 MS.gene24855:CDS 30.0%
ATACACATCAGAATTTGACT+TGG - chr8.2:6863135-6863154 MS.gene24855:CDS 30.0%
CAAAGAAAAACACTTTGCTA+AGG - chr8.2:6862571-6862590 MS.gene24855:CDS 30.0%
GATCATCATTATAAGAACCA+GGG + chr8.2:6863004-6863023 None:intergenic 30.0%
GTCAAATTCTGATGTGTATA+AGG + chr8.2:6863134-6863153 None:intergenic 30.0%
TAAGAATTCACATTGGACTT+AGG + chr8.2:6862876-6862895 None:intergenic 30.0%
TGAAATGAGAAATGAGAAGA+TGG - chr8.2:6863322-6863341 MS.gene24855:CDS 30.0%
TGATCATCATTATAAGAACC+AGG + chr8.2:6863005-6863024 None:intergenic 30.0%
TGCAATTATTGGAATATGTG+TGG + chr8.2:6862853-6862872 None:intergenic 30.0%
TGGTTCTTATAATGATGATC+AGG - chr8.2:6863004-6863023 MS.gene24855:CDS 30.0%
ACCAAAATTCCATCCATCAA+AGG + chr8.2:6863110-6863129 None:intergenic 35.0%
CAGGGAATTTGAAAGATTCT+TGG - chr8.2:6863063-6863082 MS.gene24855:CDS 35.0%
CCAAAATTCCATCCATCAAA+GGG + chr8.2:6863109-6863128 None:intergenic 35.0%
TACATTGAACCACCAACATT+TGG + chr8.2:6862828-6862847 None:intergenic 35.0%
TCAGGCCAACTAATAAACTT+TGG + chr8.2:6862753-6862772 None:intergenic 35.0%
TTGGACTTAGGTGCAATTAT+TGG + chr8.2:6862864-6862883 None:intergenic 35.0%
! AAACAGCTGATTCAAGTTCT+TGG + chr8.2:6862551-6862570 None:intergenic 35.0%
! AAGGTGTGGTTGATACTAAA+GGG - chr8.2:6862952-6862971 MS.gene24855:CDS 35.0%
! TAACAGAGTATGCAGTTTTC+TGG + chr8.2:6862926-6862945 None:intergenic 35.0%
! TTGAAAGATTCTTGGGTTGT+TGG - chr8.2:6863071-6863090 MS.gene24855:CDS 35.0%
!!! CTTTTTGGAATGCCAAATGT+TGG - chr8.2:6862813-6862832 MS.gene24855:CDS 35.0%
AAATTCTGGGTTCCCTTTGA+TGG - chr8.2:6863094-6863113 MS.gene24855:CDS 40.0%
ATGTATCAGAGAGCTATGAC+AGG - chr8.2:6863044-6863063 MS.gene24855:CDS 40.0%
GATGAAATGGTTGCTGAGAA+TGG - chr8.2:6863374-6863393 MS.gene24855:CDS 40.0%
GCATACTCTGTTACTGTTCA+AGG - chr8.2:6862933-6862952 MS.gene24855:CDS 40.0%
GCTCATGATTTATTGCATCG+TGG - chr8.2:6863437-6863456 MS.gene24855:CDS 40.0%
GGATATTCAGAAGCTGTGTA+AGG - chr8.2:6863187-6863206 MS.gene24855:CDS 40.0%
TATATCCACCTGTCATAAGC+TGG - chr8.2:6862689-6862708 MS.gene24855:CDS 40.0%
TGTATCAGAGAGCTATGACA+GGG - chr8.2:6863045-6863064 MS.gene24855:CDS 40.0%
TTGTTTGCAGAAGAGGATAG+AGG - chr8.2:6863241-6863260 MS.gene24855:CDS 40.0%
! CAAGAGTTTGAGGGTCTTTT+TGG - chr8.2:6862798-6862817 MS.gene24855:CDS 40.0%
! CAAGGTGTGGTTGATACTAA+AGG - chr8.2:6862951-6862970 MS.gene24855:CDS 40.0%
! CTTGGGTTGTTGGAAATTCT+GGG - chr8.2:6863081-6863100 MS.gene24855:CDS 40.0%
! GATGTTTTTCTTGGTTACCC+TGG - chr8.2:6862984-6863003 MS.gene24855:CDS 40.0%
! GTTTCAAAGAGGTTCGGTTT+GGG - chr8.2:6862666-6862685 MS.gene24855:CDS 40.0%
! TCTTGGGTTGTTGGAAATTC+TGG - chr8.2:6863080-6863099 MS.gene24855:CDS 40.0%
! TTTGGAATGCCAAATGTTGG+TGG - chr8.2:6862816-6862835 MS.gene24855:CDS 40.0%
!! CCCTTTGATGGATGGAATTT+TGG - chr8.2:6863106-6863125 MS.gene24855:CDS 40.0%
!! CTCTTTGAAACCAGCCTTAA+AGG + chr8.2:6862657-6862676 None:intergenic 40.0%
!! CTTTAAGGCTGGTTTCAAAG+AGG - chr8.2:6862655-6862674 MS.gene24855:CDS 40.0%
AACCAGCCTTAAAGGATCAC+CGG + chr8.2:6862649-6862668 None:intergenic 45.0%
ATGAGAAGATGGATCCTGCA+TGG - chr8.2:6863333-6863352 MS.gene24855:CDS 45.0%
CGCGTTTAATGAGAAAGTGG+AGG - chr8.2:6863166-6863185 MS.gene24855:CDS 45.0%
CGTTTGCAAAGGTGAAAGGA+AGG - chr8.2:6863213-6863232 MS.gene24855:CDS 45.0%
CTCTGTTACTGTTCAAGGTG+TGG - chr8.2:6862938-6862957 MS.gene24855:CDS 45.0%
GAGGATAGAGGTGAAGATTG+AGG - chr8.2:6863253-6863272 MS.gene24855:CDS 45.0%
GATGCGTTTGCAAAGGTGAA+AGG - chr8.2:6863209-6863228 MS.gene24855:CDS 45.0%
GCACGCGTTTAATGAGAAAG+TGG - chr8.2:6863163-6863182 MS.gene24855:CDS 45.0%
GTGTAAGGATGCGTTTGCAA+AGG - chr8.2:6863202-6863221 MS.gene24855:CDS 45.0%
TCAATAAGAGGCAGACTCAG+AGG - chr8.2:6862898-6862917 MS.gene24855:CDS 45.0%
TGTTGCTGCTGTTCAAGCTA+GGG - chr8.2:6863406-6863425 MS.gene24855:CDS 45.0%
TTATTGCATCGTGGTCGTGT+TGG - chr8.2:6863446-6863465 MS.gene24855:CDS 45.0%
TTGCAAAGGTGAAAGGAAGG+TGG - chr8.2:6863216-6863235 MS.gene24855:CDS 45.0%
TTGCTAAGGGATGCAATTCC+CGG - chr8.2:6862585-6862604 MS.gene24855:CDS 45.0%
! GGTTTCAAAGAGGTTCGGTT+TGG - chr8.2:6862665-6862684 MS.gene24855:CDS 45.0%
!! AGGCTGGTTTCAAAGAGGTT+CGG - chr8.2:6862660-6862679 MS.gene24855:CDS 45.0%
CTCGAGAACCAGCTTATGAC+AGG + chr8.2:6862700-6862719 None:intergenic 50.0%
CTGTTGCTGCTGTTCAAGCT+AGG - chr8.2:6863405-6863424 MS.gene24855:CDS 50.0%
GAGAACCAGCTTATGACAGG+TGG + chr8.2:6862697-6862716 None:intergenic 50.0%
GTGAAGATTGAGGAGTTGCC+TGG - chr8.2:6863263-6863282 MS.gene24855:CDS 50.0%
TCTGGGTTCCCTTTGATGGA+TGG - chr8.2:6863098-6863117 MS.gene24855:CDS 50.0%
TGCTAAGGGATGCAATTCCC+GGG - chr8.2:6862586-6862605 MS.gene24855:CDS 50.0%
TGTATCTGGAGACTTGCCAC+CGG - chr8.2:6862627-6862646 MS.gene24855:CDS 50.0%
TTGCCACCGGTGATCCTTTA+AGG - chr8.2:6862640-6862659 MS.gene24855:CDS 50.0%
! CTGTCATAAGCTGGTTCTCG+AGG - chr8.2:6862698-6862717 MS.gene24855:CDS 50.0%
!! GGTGGTCTTGTTTGCAGAAG+AGG - chr8.2:6863234-6863253 MS.gene24855:CDS 50.0%
CAGCAAGCACCAAGAACACC+AGG + chr8.2:6863284-6863303 None:intergenic 55.0%
CAGCCTTAAAGGATCACCGG+TGG + chr8.2:6862646-6862665 None:intergenic 55.0%
GAGGAGTTGCCTGGTGTTCT+TGG - chr8.2:6863272-6863291 MS.gene24855:CDS 55.0%
TAAGGGATGCAATTCCCGGG+CGG - chr8.2:6862589-6862608 MS.gene24855:CDS 55.0%
!! CACCGGTGATCCTTTAAGGC+TGG - chr8.2:6862644-6862663 MS.gene24855:CDS 55.0%
AGCGCGTGGCGGTTTGTATC+TGG - chr8.2:6862613-6862632 MS.gene24855:CDS 60.0%
AATTCCCGGGCGGCAGCGCG+TGG - chr8.2:6862599-6862618 MS.gene24855:CDS 75.0%
! AACCGCCACGCGCTGCCGCC+CGG + chr8.2:6862607-6862626 None:intergenic 80.0%
! ACCGCCACGCGCTGCCGCCC+GGG + chr8.2:6862606-6862625 None:intergenic 85.0%
! TCCCGGGCGGCAGCGCGTGG+CGG - chr8.2:6862602-6862621 MS.gene24855:CDS 85.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 6862519 6863481 6862519 ID=MS.gene24855
chr8.2 mRNA 6862519 6863481 6862519 ID=MS.gene24855.t1;Parent=MS.gene24855
chr8.2 exon 6862519 6863481 6862519 ID=MS.gene24855.t1.exon1;Parent=MS.gene24855.t1
chr8.2 CDS 6862519 6863481 6862519 ID=cds.MS.gene24855.t1;Parent=MS.gene24855.t1
Gene Sequence

>MS.gene24855

ATGAGCAAAGCAACGTTCGAGTTTATATGCCAAGAACTTGAATCAGCTGTTTCAAAGAAAAACACTTTGCTAAGGGATGCAATTCCCGGGCGGCAGCGCGTGGCGGTTTGTATCTGGAGACTTGCCACCGGTGATCCTTTAAGGCTGGTTTCAAAGAGGTTCGGTTTGGGTATATCCACCTGTCATAAGCTGGTTCTCGAGGTCTGTGCTGCAATCAAGAAAGTTCTTATGCCAAAGTTTATTAGTTGGCCTGATGAAAATAAGATGAATTTGATTAAACAAGAGTTTGAGGGTCTTTTTGGAATGCCAAATGTTGGTGGTTCAATGTATACCACACATATTCCAATAATTGCACCTAAGTCCAATGTGAATTCTTATTTCAATAAGAGGCAGACTCAGAGGAACCAGAAAACTGCATACTCTGTTACTGTTCAAGGTGTGGTTGATACTAAAGGGATTTTCACTGATGTTTTTCTTGGTTACCCTGGTTCTTATAATGATGATCAGGTTTTGGAGAAATCTGTAATGTATCAGAGAGCTATGACAGGGAATTTGAAAGATTCTTGGGTTGTTGGAAATTCTGGGTTCCCTTTGATGGATGGAATTTTGGTTCCTTATACACATCAGAATTTGACTTGGACACAGCACGCGTTTAATGAGAAAGTGGAGGATATTCAGAAGCTGTGTAAGGATGCGTTTGCAAAGGTGAAAGGAAGGTGGTCTTGTTTGCAGAAGAGGATAGAGGTGAAGATTGAGGAGTTGCCTGGTGTTCTTGGTGCTTGCTGTGTTCTGCATAATATCTGTGAAATGAGAAATGAGAAGATGGATCCTGCATGGAATTTTGAACTTTTTGATGATGAAATGGTTGCTGAGAATGGTGTAAGATCTGTTGCTGCTGTTCAAGCTAGGGATAATATTGCTCATGATTTATTGCATCGTGGTCGTGTTGGCAATACTTTTTAA

Protein sequence

>MS.gene24855.t1

MSKATFEFICQELESAVSKKNTLLRDAIPGRQRVAVCIWRLATGDPLRLVSKRFGLGISTCHKLVLEVCAAIKKVLMPKFISWPDENKMNLIKQEFEGLFGMPNVGGSMYTTHIPIIAPKSNVNSYFNKRQTQRNQKTAYSVTVQGVVDTKGIFTDVFLGYPGSYNDDQVLEKSVMYQRAMTGNLKDSWVVGNSGFPLMDGILVPYTHQNLTWTQHAFNEKVEDIQKLCKDAFAKVKGRWSCLQKRIEVKIEELPGVLGACCVLHNICEMRNEKMDPAWNFELFDDEMVAENGVRSVAAVQARDNIAHDLLHRGRVGNTF