Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04927.t1 | XP_003600750.2 | 81.1 | 122 | 20 | 1 | 1 | 119 | 19 | 140 | 4.70E-54 | 220.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04927.t1 | P62926 | 45.7 | 116 | 53 | 4 | 1 | 114 | 18 | 125 | 1.3e-20 | 100.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04927.t1 | G7J9G6 | 81.1 | 122 | 20 | 1 | 1 | 119 | 19 | 140 | 3.4e-54 | 220.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04927 | MS.gene08901 | 0.816315 | 5.89E-52 | -1.69E-46 |
| MS.gene04927 | MS.gene08902 | 0.821568 | 3.77E-53 | -1.69E-46 |
| MS.gene04927 | MS.gene29765 | 0.803169 | 3.96E-49 | -1.69E-46 |
| MS.gene04927 | MS.gene33698 | 0.808553 | 2.93E-50 | -1.69E-46 |
| MS.gene04927 | MS.gene38435 | 0.819782 | 9.69E-53 | -1.69E-46 |
| MS.gene04927 | MS.gene38815 | 0.811547 | 6.63E-51 | -1.69E-46 |
| MS.gene04927 | MS.gene50279 | 0.800056 | 1.72E-48 | -1.69E-46 |
| MS.gene04927 | MS.gene51595 | 0.800079 | 1.71E-48 | -1.69E-46 |
| MS.gene04927 | MS.gene63066 | 0.927006 | 2.14E-91 | -1.69E-46 |
| MS.gene04927 | MS.gene99062 | 0.820542 | 6.49E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04927.t1 | MTR_3g067430 | 81.148 | 122 | 20 | 1 | 1 | 119 | 19 | 140 | 1.08e-70 | 208 |
| MS.gene04927.t1 | MTR_3g067445 | 80.808 | 99 | 16 | 1 | 1 | 96 | 19 | 117 | 1.66e-56 | 171 |
| MS.gene04927.t1 | MTR_3g067540 | 53.279 | 122 | 54 | 1 | 1 | 119 | 19 | 140 | 2.88e-39 | 128 |
| MS.gene04927.t1 | MTR_3g067555 | 53.279 | 122 | 54 | 1 | 1 | 119 | 19 | 140 | 2.88e-39 | 128 |
| MS.gene04927.t1 | MTR_3g067555 | 53.279 | 122 | 54 | 1 | 1 | 119 | 6 | 127 | 3.17e-39 | 128 |
| MS.gene04927.t1 | MTR_3g067550 | 52.941 | 119 | 53 | 1 | 4 | 119 | 1 | 119 | 6.31e-37 | 122 |
| MS.gene04927.t1 | MTR_3g067555 | 52.941 | 119 | 53 | 1 | 4 | 119 | 1 | 119 | 6.31e-37 | 122 |
| MS.gene04927.t1 | MTR_3g067510 | 53.719 | 121 | 45 | 4 | 1 | 119 | 24 | 135 | 5.44e-36 | 120 |
| MS.gene04927.t1 | MTR_3g067510 | 53.719 | 121 | 45 | 4 | 1 | 119 | 19 | 130 | 5.78e-36 | 119 |
| MS.gene04927.t1 | MTR_3g067437 | 53.448 | 116 | 45 | 3 | 1 | 112 | 19 | 129 | 7.63e-36 | 119 |
| MS.gene04927.t1 | MTR_3g067535 | 55.285 | 123 | 46 | 3 | 1 | 119 | 19 | 136 | 8.77e-36 | 119 |
| MS.gene04927.t1 | MTR_3g067580 | 51.220 | 123 | 51 | 3 | 1 | 119 | 27 | 144 | 1.50e-35 | 119 |
| MS.gene04927.t1 | MTR_3g067270 | 54.701 | 117 | 46 | 2 | 1 | 115 | 19 | 130 | 1.76e-33 | 114 |
| MS.gene04927.t1 | MTR_3g467750 | 54.701 | 117 | 46 | 2 | 1 | 115 | 19 | 130 | 1.76e-33 | 114 |
| MS.gene04927.t1 | MTR_3g463570 | 52.500 | 120 | 53 | 2 | 1 | 119 | 20 | 136 | 5.31e-33 | 112 |
| MS.gene04927.t1 | MTR_3g067280 | 56.897 | 116 | 45 | 4 | 1 | 115 | 19 | 130 | 1.27e-32 | 111 |
| MS.gene04927.t1 | MTR_3g463570 | 52.500 | 120 | 53 | 2 | 1 | 119 | 40 | 156 | 1.38e-32 | 112 |
| MS.gene04927.t1 | MTR_3g467760 | 53.043 | 115 | 27 | 2 | 1 | 115 | 19 | 106 | 3.17e-32 | 109 |
| MS.gene04927.t1 | MTR_3g067500 | 55.000 | 120 | 44 | 4 | 4 | 119 | 1 | 114 | 3.83e-32 | 109 |
| MS.gene04927.t1 | MTR_3g067830 | 67.857 | 84 | 23 | 2 | 1 | 81 | 19 | 101 | 6.31e-32 | 108 |
| MS.gene04927.t1 | MTR_3g067570 | 57.292 | 96 | 37 | 3 | 4 | 99 | 1 | 92 | 2.29e-29 | 102 |
| MS.gene04927.t1 | MTR_6g036620 | 47.934 | 121 | 56 | 3 | 1 | 119 | 19 | 134 | 3.51e-29 | 102 |
| MS.gene04927.t1 | MTR_7g056817 | 47.581 | 124 | 57 | 4 | 1 | 119 | 19 | 139 | 2.02e-28 | 100 |
| MS.gene04927.t1 | MTR_7g056803 | 43.443 | 122 | 64 | 3 | 1 | 119 | 19 | 138 | 7.76e-27 | 97.1 |
| MS.gene04927.t1 | MTR_6g017150 | 48.305 | 118 | 53 | 5 | 2 | 115 | 20 | 133 | 5.96e-26 | 94.7 |
| MS.gene04927.t1 | MTR_6g017170 | 46.429 | 112 | 52 | 5 | 1 | 108 | 19 | 126 | 8.86e-25 | 91.3 |
| MS.gene04927.t1 | MTR_8g022430 | 42.857 | 119 | 60 | 5 | 1 | 115 | 19 | 133 | 2.73e-23 | 88.2 |
| MS.gene04927.t1 | MTR_8g022400 | 44.068 | 118 | 58 | 6 | 1 | 115 | 20 | 132 | 1.66e-22 | 86.3 |
| MS.gene04927.t1 | MTR_8g022420 | 43.478 | 115 | 58 | 5 | 1 | 112 | 19 | 129 | 3.07e-22 | 85.5 |
| MS.gene04927.t1 | MTR_6g047880 | 42.735 | 117 | 63 | 3 | 1 | 115 | 19 | 133 | 5.93e-22 | 84.3 |
| MS.gene04927.t1 | MTR_3g436100 | 38.235 | 136 | 61 | 4 | 1 | 117 | 19 | 150 | 2.20e-21 | 83.6 |
| MS.gene04927.t1 | MTR_4g026590 | 43.548 | 124 | 57 | 6 | 1 | 115 | 20 | 139 | 1.92e-19 | 78.2 |
| MS.gene04927.t1 | MTR_7g044920 | 38.806 | 134 | 62 | 4 | 1 | 117 | 18 | 148 | 2.39e-19 | 78.2 |
| MS.gene04927.t1 | MTR_7g044980 | 37.795 | 127 | 62 | 3 | 3 | 117 | 20 | 141 | 3.08e-19 | 77.8 |
| MS.gene04927.t1 | MTR_6g047900 | 39.655 | 116 | 65 | 3 | 1 | 115 | 19 | 130 | 1.44e-17 | 73.2 |
| MS.gene04927.t1 | MTR_8g461020 | 38.235 | 102 | 56 | 2 | 1 | 100 | 20 | 116 | 4.46e-17 | 72.0 |
| MS.gene04927.t1 | MTR_1g019650 | 38.679 | 106 | 50 | 2 | 1 | 105 | 19 | 110 | 1.24e-15 | 67.8 |
| MS.gene04927.t1 | MTR_6g082060 | 34.513 | 113 | 64 | 4 | 1 | 106 | 19 | 128 | 1.82e-13 | 62.8 |
| MS.gene04927.t1 | MTR_8g056800 | 37.500 | 96 | 48 | 2 | 33 | 117 | 3 | 97 | 2.85e-13 | 61.2 |
| MS.gene04927.t1 | MTR_7g029540 | 40.000 | 105 | 57 | 4 | 1 | 103 | 21 | 121 | 1.77e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTCTTTCTTGAATTTAT+AGG | 0.213981 | 3.4:+67731425 | None:intergenic |
| CCAAATTCTAATCTCAAATA+TGG | 0.221865 | 3.4:-67731501 | MS.gene04927:CDS |
| ATAATCATTGATTGTACTTT+TGG | 0.228048 | 3.4:-67731651 | MS.gene04927:CDS |
| CCATATTTGAGATTAGAATT+TGG | 0.234945 | 3.4:+67731501 | None:intergenic |
| TTTCTTGAATTTATAGGTAG+AGG | 0.443826 | 3.4:+67731431 | None:intergenic |
| TCATGAGACCAACATAACTT+TGG | 0.463675 | 3.4:+67731567 | None:intergenic |
| TTTCCGATGAAGAAAGTAGA+AGG | 0.469210 | 3.4:-67731735 | MS.gene04927:CDS |
| GCTCCTTCTACTTTCTTCAT+CGG | 0.489698 | 3.4:+67731732 | None:intergenic |
| GCTAGGACAGCCTGGCGAAA+AGG | 0.500319 | 3.4:+67731686 | None:intergenic |
| TCACAACCGCTAGGACAGCC+TGG | 0.515758 | 3.4:+67731678 | None:intergenic |
| ATGAAGAAAGTAGAAGGAGC+AGG | 0.537028 | 3.4:-67731729 | MS.gene04927:CDS |
| AGGAACATCCAAAGTTATGT+TGG | 0.542709 | 3.4:-67731575 | MS.gene04927:CDS |
| AGCACAAGACGCATACAAGA+TGG | 0.606334 | 3.4:-67731454 | MS.gene04927:CDS |
| ATTATGCATTCACAACCGCT+AGG | 0.655067 | 3.4:+67731669 | None:intergenic |
| GTCTTGTGCTTCAGAGACAG+AGG | 0.689585 | 3.4:+67731467 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATCATTGATTGTACTTT+TGG | - | chr3.4:67731488-67731507 | MS.gene04927:CDS | 20.0% |
| ! | CCAAATTCTAATCTCAAATA+TGG | - | chr3.4:67731638-67731657 | MS.gene04927:CDS | 25.0% |
| ! | GAAGTCTTTCTTGAATTTAT+AGG | + | chr3.4:67731717-67731736 | None:intergenic | 25.0% |
| ! | TTTCTTGAATTTATAGGTAG+AGG | + | chr3.4:67731711-67731730 | None:intergenic | 25.0% |
| !! | CCATATTTGAGATTAGAATT+TGG | + | chr3.4:67731641-67731660 | None:intergenic | 25.0% |
| CATGATGAATGCACAAAAAA+GGG | - | chr3.4:67731590-67731609 | MS.gene04927:CDS | 30.0% | |
| TCATGATGAATGCACAAAAA+AGG | - | chr3.4:67731589-67731608 | MS.gene04927:CDS | 30.0% | |
| ! | GTTTTTGCGAAAATGACAAT+GGG | - | chr3.4:67731522-67731541 | MS.gene04927:CDS | 30.0% |
| ! | TGTACTTTTGGTATTTGTGT+GGG | - | chr3.4:67731500-67731519 | MS.gene04927:CDS | 30.0% |
| ! | TTGTACTTTTGGTATTTGTG+TGG | - | chr3.4:67731499-67731518 | MS.gene04927:CDS | 30.0% |
| AATGCACAAAAAAGGGTAGT+GGG | - | chr3.4:67731597-67731616 | MS.gene04927:CDS | 35.0% | |
| AGGAACATCCAAAGTTATGT+TGG | - | chr3.4:67731564-67731583 | MS.gene04927:CDS | 35.0% | |
| TCATGAGACCAACATAACTT+TGG | + | chr3.4:67731575-67731594 | None:intergenic | 35.0% | |
| TTTCCGATGAAGAAAGTAGA+AGG | - | chr3.4:67731404-67731423 | MS.gene04927:CDS | 35.0% | |
| ! | ATGACAATGGGAATTTTCAG+AGG | - | chr3.4:67731534-67731553 | MS.gene04927:CDS | 35.0% |
| ! | GGTTTTTGCGAAAATGACAA+TGG | - | chr3.4:67731521-67731540 | MS.gene04927:CDS | 35.0% |
| ATGAAGAAAGTAGAAGGAGC+AGG | - | chr3.4:67731410-67731429 | MS.gene04927:CDS | 40.0% | |
| ATTATGCATTCACAACCGCT+AGG | + | chr3.4:67731473-67731492 | None:intergenic | 40.0% | |
| GAATGCACAAAAAAGGGTAG+TGG | - | chr3.4:67731596-67731615 | MS.gene04927:CDS | 40.0% | |
| GCTCCTTCTACTTTCTTCAT+CGG | + | chr3.4:67731410-67731429 | None:intergenic | 40.0% | |
| ! | CAATGGGAATTTTCAGAGGA+AGG | - | chr3.4:67731538-67731557 | MS.gene04927:CDS | 40.0% |
| ! | GAATTTTCAGAGGAAGGTTG+AGG | - | chr3.4:67731544-67731563 | MS.gene04927:CDS | 40.0% |
| AGCACAAGACGCATACAAGA+TGG | - | chr3.4:67731685-67731704 | MS.gene04927:CDS | 45.0% | |
| GTCTTGTGCTTCAGAGACAG+AGG | + | chr3.4:67731675-67731694 | None:intergenic | 50.0% | |
| ! | AGTTGCAGTACCTTTTCGCC+AGG | - | chr3.4:67731443-67731462 | MS.gene04927:CDS | 50.0% |
| ! | TTTTCGCCAGGCTGTCCTAG+CGG | - | chr3.4:67731455-67731474 | MS.gene04927:CDS | 55.0% |
| GCTAGGACAGCCTGGCGAAA+AGG | + | chr3.4:67731456-67731475 | None:intergenic | 60.0% | |
| TCACAACCGCTAGGACAGCC+TGG | + | chr3.4:67731464-67731483 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 67731401 | 67731760 | 67731401 | ID=MS.gene04927 |
| chr3.4 | mRNA | 67731401 | 67731760 | 67731401 | ID=MS.gene04927.t1;Parent=MS.gene04927 |
| chr3.4 | exon | 67731401 | 67731760 | 67731401 | ID=MS.gene04927.t1.exon1;Parent=MS.gene04927.t1 |
| chr3.4 | CDS | 67731401 | 67731760 | 67731401 | ID=cds.MS.gene04927.t1;Parent=MS.gene04927.t1 |
| Gene Sequence |
| Protein sequence |
