Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050467.t1 | XP_013450528.1 | 93.7 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 1.70E-112 | 415.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050467.t1 | P02877 | 71.1 | 201 | 51 | 3 | 6 | 202 | 1 | 198 | 5.9e-85 | 315.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050467.t1 | A0A072U5A4 | 93.7 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 1.3e-112 | 415.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050467 | MS.gene050532 | 0.993785 | 3.34E-202 | -1.69E-46 |
| MS.gene050467 | MS.gene053826 | 0.811478 | 6.86E-51 | -1.69E-46 |
| MS.gene050467 | MS.gene058171 | 0.803549 | 3.31E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene058172 | 0.80304 | 4.21E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene058173 | 0.803517 | 3.36E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene060187 | 0.813107 | 3.03E-51 | -1.69E-46 |
| MS.gene050467 | MS.gene23406 | 0.817613 | 3.01E-52 | -1.69E-46 |
| MS.gene050467 | MS.gene36282 | 0.803747 | 3.01E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene61320 | 0.804734 | 1.87E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene63052 | 0.807519 | 4.86E-50 | -1.69E-46 |
| MS.gene050467 | MS.gene63751 | 0.804471 | 2.13E-49 | -1.69E-46 |
| MS.gene050467 | MS.gene89097 | 0.818321 | 2.08E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050467.t1 | MTR_7g115220 | 93.689 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 8.93e-136 | 379 |
| MS.gene050467.t1 | MTR_1g080820 | 71.901 | 121 | 34 | 0 | 68 | 188 | 24 | 144 | 5.76e-64 | 194 |
| MS.gene050467.t1 | MTR_1g080810 | 71.074 | 121 | 35 | 0 | 68 | 188 | 24 | 144 | 2.55e-63 | 193 |
| MS.gene050467.t1 | MTR_1g080780 | 70.000 | 120 | 36 | 0 | 71 | 190 | 22 | 141 | 4.17e-63 | 192 |
| MS.gene050467.t1 | MTR_1g080800 | 70.732 | 123 | 36 | 0 | 68 | 190 | 24 | 146 | 1.18e-62 | 191 |
| MS.gene050467.t1 | MTR_6g083270 | 50.000 | 154 | 60 | 2 | 61 | 197 | 15 | 168 | 1.52e-43 | 151 |
| MS.gene050467.t1 | MTR_6g083200 | 47.692 | 130 | 64 | 2 | 61 | 188 | 15 | 142 | 2.65e-38 | 131 |
| MS.gene050467.t1 | MTR_6g083290 | 47.445 | 137 | 61 | 2 | 61 | 190 | 15 | 147 | 1.60e-34 | 124 |
| MS.gene050467.t1 | MTR_6g083310 | 42.667 | 150 | 69 | 3 | 66 | 200 | 20 | 167 | 1.26e-33 | 118 |
| MS.gene050467.t1 | MTR_3g118390 | 51.515 | 66 | 32 | 0 | 1 | 66 | 59 | 124 | 8.51e-17 | 77.8 |
| MS.gene050467.t1 | MTR_8g074335 | 68.293 | 41 | 13 | 0 | 22 | 62 | 22 | 62 | 7.48e-13 | 66.2 |
| MS.gene050467.t1 | MTR_8g074350 | 50.000 | 56 | 28 | 0 | 6 | 61 | 7 | 62 | 8.66e-12 | 63.2 |
| MS.gene050467.t1 | MTR_8g074330 | 67.500 | 40 | 13 | 0 | 22 | 61 | 22 | 61 | 1.50e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050467.t1 | AT3G04720 | 71.292 | 209 | 52 | 3 | 3 | 203 | 2 | 210 | 3.56e-97 | 281 |
| MS.gene050467.t1 | AT3G12500 | 68.293 | 41 | 12 | 1 | 22 | 61 | 33 | 73 | 8.44e-12 | 63.5 |
Find 59 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTAGACATGGGAAAATT+TGG | 0.218092 | 7.3:-2269260 | None:intergenic |
| ATGATGCAGGTGACAAATTC+AGG | 0.293677 | 7.3:-2268670 | MS.gene050467:intron |
| CAGCAATGGAGGGTTGGATT+TGG | 0.326401 | 7.3:-2268608 | MS.gene050467:CDS |
| TACTCATGGCGTAGCAAGTA+TGG | 0.353266 | 7.3:-2268936 | MS.gene050467:CDS |
| CTTCTTGTGGAAAGTGTCTC+AGG | 0.353594 | 7.3:-2268872 | MS.gene050467:CDS |
| TGGCTGCAACAAAGGTTGTT+TGG | 0.365069 | 7.3:+2269149 | None:intergenic |
| GCTTGACCACGAGGTCCAAC+AGG | 0.397549 | 7.3:+2268894 | None:intergenic |
| GGAGGGTTGGATTTGGATGT+AGG | 0.409363 | 7.3:-2268601 | MS.gene050467:CDS |
| AAGTCCCATCCATGTTGCTC+TGG | 0.422201 | 7.3:+2269005 | None:intergenic |
| TATTGTAAGCTATCAGTTTG+TGG | 0.428774 | 7.3:-2268527 | MS.gene050467:CDS |
| GCTATCAGTTTGTGGATTGT+GGG | 0.462963 | 7.3:-2268519 | MS.gene050467:CDS |
| AGATGGCAGAGGATACCAAC+AGG | 0.473028 | 7.3:-2268557 | MS.gene050467:CDS |
| AACCTTTGTTGCAGCCAATA+CGG | 0.476377 | 7.3:-2269143 | MS.gene050467:CDS |
| TACTTGCTACGCCATGAGTA+AGG | 0.479661 | 7.3:+2268939 | None:intergenic |
| AACCGTATTGGCTGCAACAA+AGG | 0.489204 | 7.3:+2269141 | None:intergenic |
| AAATTCAGGGACGGGAGCAC+AGG | 0.490592 | 7.3:-2268656 | MS.gene050467:CDS |
| AGCTATCAGTTTGTGGATTG+TGG | 0.492406 | 7.3:-2268520 | MS.gene050467:CDS |
| GAACAATGCGGTAAACAAGC+TGG | 0.511319 | 7.3:-2269185 | MS.gene050467:CDS |
| TTATTGTTCAGCATCAATGA+TGG | 0.515481 | 7.3:+2268465 | None:intergenic |
| TGGTACCACACCAACCGTAT+TGG | 0.526780 | 7.3:+2269129 | None:intergenic |
| TAAGTGCTTATTGTTCAACT+TGG | 0.529463 | 7.3:-2268974 | MS.gene050467:CDS |
| TGACAATTACTCTGACAGTT+CGG | 0.538086 | 7.3:+2269089 | None:intergenic |
| CAGGTGACAAATTCAGGGAC+GGG | 0.545443 | 7.3:-2268664 | MS.gene050467:CDS |
| CCTCGTGGTCAAGCTTCTTG+TGG | 0.556286 | 7.3:-2268885 | MS.gene050467:CDS |
| TCAATGCAGCAATGGAGGGT+TGG | 0.558488 | 7.3:-2268614 | MS.gene050467:CDS |
| AAGTGCTTATTGTTCAACTT+GGG | 0.564174 | 7.3:-2268973 | MS.gene050467:CDS |
| AATGATGGAGAACAGAGGAT+TGG | 0.564526 | 7.3:+2268480 | None:intergenic |
| GTAGATCAATGCAGCAATGG+AGG | 0.578522 | 7.3:-2268619 | MS.gene050467:CDS |
| TTTGTTGCAGCCAATACGGT+TGG | 0.578744 | 7.3:-2269139 | MS.gene050467:CDS |
| TGATGCAGGTGACAAATTCA+GGG | 0.583955 | 7.3:-2268669 | MS.gene050467:intron |
| ATGCAAGCAAACCTTACTCA+TGG | 0.586811 | 7.3:-2268950 | MS.gene050467:CDS |
| ATCAACCAGAGCAACATGGA+TGG | 0.587569 | 7.3:-2269010 | MS.gene050467:CDS |
| TCAACCAGAGCAACATGGAT+GGG | 0.598724 | 7.3:-2269009 | MS.gene050467:CDS |
| CATGGCGTAGCAAGTATGGT+TGG | 0.600676 | 7.3:-2268932 | MS.gene050467:CDS |
| TAGATCAATGCAGCAATGGA+GGG | 0.603290 | 7.3:-2268618 | MS.gene050467:CDS |
| TTACAATAAGATGTCCCTGT+TGG | 0.604159 | 7.3:+2268542 | None:intergenic |
| GCAGGTGACAAATTCAGGGA+CGG | 0.605105 | 7.3:-2268665 | MS.gene050467:intron |
| AGTAATTGTCATGGCAGTAG+TGG | 0.614410 | 7.3:-2269077 | MS.gene050467:CDS |
| TGTGGCCCTGTTGGACCTCG+TGG | 0.621769 | 7.3:-2268900 | MS.gene050467:CDS |
| TGTCATGGCAGTAGTGGTGG+TGG | 0.621989 | 7.3:-2269071 | MS.gene050467:CDS |
| TTCTTGTGGAAAGTGTCTCA+GGG | 0.622227 | 7.3:-2268871 | MS.gene050467:intron |
| AATTGTCATGGCAGTAGTGG+TGG | 0.624496 | 7.3:-2269074 | MS.gene050467:CDS |
| AAGCCTAGCGGTATTAACAT+TGG | 0.627981 | 7.3:-2269234 | MS.gene050467:CDS |
| AACTGTCAGAGTAATTGTCA+TGG | 0.629228 | 7.3:-2269086 | MS.gene050467:CDS |
| ATTGTAGATCAATGCAGCAA+TGG | 0.636888 | 7.3:-2268622 | MS.gene050467:CDS |
| TACTATCAACCAGAGCAACA+TGG | 0.648851 | 7.3:-2269014 | MS.gene050467:CDS |
| TGCAGCCAATACGGTTGGTG+TGG | 0.651269 | 7.3:-2269134 | MS.gene050467:CDS |
| TTTAACAGAATCGACACAGA+TGG | 0.651956 | 7.3:-2268574 | MS.gene050467:CDS |
| CCACAAGAAGCTTGACCACG+AGG | 0.656609 | 7.3:+2268885 | None:intergenic |
| AGAATCGACACAGATGGCAG+AGG | 0.657142 | 7.3:-2268568 | MS.gene050467:CDS |
| ACACCAATGTTAATACCGCT+AGG | 0.661884 | 7.3:+2269231 | None:intergenic |
| CAATGCGGTAAACAAGCTGG+AGG | 0.695826 | 7.3:-2269182 | MS.gene050467:CDS |
| CTTGACCACGAGGTCCAACA+GGG | 0.697195 | 7.3:+2268895 | None:intergenic |
| CGGACCACAGTATTCATCTG+TGG | 0.698610 | 7.3:+2269109 | None:intergenic |
| GCATCAATGATGGAGAACAG+AGG | 0.711827 | 7.3:+2268475 | None:intergenic |
| GATGGCAGAGGATACCAACA+GGG | 0.745988 | 7.3:-2268556 | MS.gene050467:CDS |
| ACAACAGTTGCAGAACAATG+CGG | 0.746680 | 7.3:-2269197 | MS.gene050467:CDS |
| AAAATTTGGAGTAAGCCTAG+CGG | 0.781644 | 7.3:-2269246 | MS.gene050467:CDS |
| GGTACCACAGATGAATACTG+TGG | 0.812966 | 7.3:-2269113 | MS.gene050467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGAGTAAATATAGATAAAA+AGG | + | chr7.3:2268902-2268921 | None:intergenic | 15.0% |
| !!! | TACTCTAATGTTTTAAAAAT+AGG | - | chr7.3:2268915-2268934 | MS.gene050467:CDS | 15.0% |
| !! | TAGATAAAAAGGAATAGAAT+TGG | + | chr7.3:2268891-2268910 | None:intergenic | 20.0% |
| AAGTGCTTATTGTTCAACTT+GGG | - | chr7.3:2268743-2268762 | MS.gene050467:intron | 30.0% | |
| TAAGTGCTTATTGTTCAACT+TGG | - | chr7.3:2268742-2268761 | MS.gene050467:intron | 30.0% | |
| TATTGTAAGCTATCAGTTTG+TGG | - | chr7.3:2269189-2269208 | MS.gene050467:CDS | 30.0% | |
| TTATTGTTCAGCATCAATGA+TGG | + | chr7.3:2269254-2269273 | None:intergenic | 30.0% | |
| AACTGTCAGAGTAATTGTCA+TGG | - | chr7.3:2268630-2268649 | MS.gene050467:CDS | 35.0% | |
| ATTGTAGATCAATGCAGCAA+TGG | - | chr7.3:2269094-2269113 | MS.gene050467:CDS | 35.0% | |
| TGACAATTACTCTGACAGTT+CGG | + | chr7.3:2268630-2268649 | None:intergenic | 35.0% | |
| TTACAATAAGATGTCCCTGT+TGG | + | chr7.3:2269177-2269196 | None:intergenic | 35.0% | |
| TTTAACAGAATCGACACAGA+TGG | - | chr7.3:2269142-2269161 | MS.gene050467:CDS | 35.0% | |
| ! | AAAATTTGGAGTAAGCCTAG+CGG | - | chr7.3:2268470-2268489 | MS.gene050467:CDS | 35.0% |
| ! | GAATGTAAATGTGATGATGC+AGG | - | chr7.3:2269033-2269052 | MS.gene050467:CDS | 35.0% |
| AACCTTTGTTGCAGCCAATA+CGG | - | chr7.3:2268573-2268592 | MS.gene050467:CDS | 40.0% | |
| AAGCCTAGCGGTATTAACAT+TGG | - | chr7.3:2268482-2268501 | MS.gene050467:CDS | 40.0% | |
| ACAACAGTTGCAGAACAATG+CGG | - | chr7.3:2268519-2268538 | MS.gene050467:CDS | 40.0% | |
| ACACCAATGTTAATACCGCT+AGG | + | chr7.3:2268488-2268507 | None:intergenic | 40.0% | |
| AGTAATTGTCATGGCAGTAG+TGG | - | chr7.3:2268639-2268658 | MS.gene050467:CDS | 40.0% | |
| ATGATGCAGGTGACAAATTC+AGG | - | chr7.3:2269046-2269065 | MS.gene050467:CDS | 40.0% | |
| ATGCAAGCAAACCTTACTCA+TGG | - | chr7.3:2268766-2268785 | MS.gene050467:intron | 40.0% | |
| TACTATCAACCAGAGCAACA+TGG | - | chr7.3:2268702-2268721 | MS.gene050467:intron | 40.0% | |
| TAGATCAATGCAGCAATGGA+GGG | - | chr7.3:2269098-2269117 | MS.gene050467:CDS | 40.0% | |
| TGATGCAGGTGACAAATTCA+GGG | - | chr7.3:2269047-2269066 | MS.gene050467:CDS | 40.0% | |
| ! | AATGATGGAGAACAGAGGAT+TGG | + | chr7.3:2269239-2269258 | None:intergenic | 40.0% |
| ! | AGCTATCAGTTTGTGGATTG+TGG | - | chr7.3:2269196-2269215 | MS.gene050467:CDS | 40.0% |
| ! | GCTATCAGTTTGTGGATTGT+GGG | - | chr7.3:2269197-2269216 | MS.gene050467:CDS | 40.0% |
| !! | TTCTTGTGGAAAGTGTCTCA+GGG | - | chr7.3:2268845-2268864 | MS.gene050467:intron | 40.0% |
| !!! | TATGGTTGGACTGCTTTTTG+TGG | - | chr7.3:2268798-2268817 | MS.gene050467:intron | 40.0% |
| AATTGTCATGGCAGTAGTGG+TGG | - | chr7.3:2268642-2268661 | MS.gene050467:CDS | 45.0% | |
| ATCAACCAGAGCAACATGGA+TGG | - | chr7.3:2268706-2268725 | MS.gene050467:intron | 45.0% | |
| GAACAATGCGGTAAACAAGC+TGG | - | chr7.3:2268531-2268550 | MS.gene050467:CDS | 45.0% | |
| GGTACCACAGATGAATACTG+TGG | - | chr7.3:2268603-2268622 | MS.gene050467:CDS | 45.0% | |
| GTAGATCAATGCAGCAATGG+AGG | - | chr7.3:2269097-2269116 | MS.gene050467:CDS | 45.0% | |
| TACTCATGGCGTAGCAAGTA+TGG | - | chr7.3:2268780-2268799 | MS.gene050467:intron | 45.0% | |
| TCAACCAGAGCAACATGGAT+GGG | - | chr7.3:2268707-2268726 | MS.gene050467:intron | 45.0% | |
| TGGCTGCAACAAAGGTTGTT+TGG | + | chr7.3:2268570-2268589 | None:intergenic | 45.0% | |
| TTTGTTGCAGCCAATACGGT+TGG | - | chr7.3:2268577-2268596 | MS.gene050467:CDS | 45.0% | |
| ! | GCATCAATGATGGAGAACAG+AGG | + | chr7.3:2269244-2269263 | None:intergenic | 45.0% |
| ! | TACTTGCTACGCCATGAGTA+AGG | + | chr7.3:2268780-2268799 | None:intergenic | 45.0% |
| !! | AACCGTATTGGCTGCAACAA+AGG | + | chr7.3:2268578-2268597 | None:intergenic | 45.0% |
| !! | CTTCTTGTGGAAAGTGTCTC+AGG | - | chr7.3:2268844-2268863 | MS.gene050467:intron | 45.0% |
| AAGTCCCATCCATGTTGCTC+TGG | + | chr7.3:2268714-2268733 | None:intergenic | 50.0% | |
| AGAATCGACACAGATGGCAG+AGG | - | chr7.3:2269148-2269167 | MS.gene050467:CDS | 50.0% | |
| AGATGGCAGAGGATACCAAC+AGG | - | chr7.3:2269159-2269178 | MS.gene050467:CDS | 50.0% | |
| CAATGCGGTAAACAAGCTGG+AGG | - | chr7.3:2268534-2268553 | MS.gene050467:CDS | 50.0% | |
| CAGCAATGGAGGGTTGGATT+TGG | - | chr7.3:2269108-2269127 | MS.gene050467:CDS | 50.0% | |
| CAGGTGACAAATTCAGGGAC+GGG | - | chr7.3:2269052-2269071 | MS.gene050467:CDS | 50.0% | |
| CATGGCGTAGCAAGTATGGT+TGG | - | chr7.3:2268784-2268803 | MS.gene050467:intron | 50.0% | |
| CGGACCACAGTATTCATCTG+TGG | + | chr7.3:2268610-2268629 | None:intergenic | 50.0% | |
| GATGGCAGAGGATACCAACA+GGG | - | chr7.3:2269160-2269179 | MS.gene050467:CDS | 50.0% | |
| GCAGGTGACAAATTCAGGGA+CGG | - | chr7.3:2269051-2269070 | MS.gene050467:CDS | 50.0% | |
| GGAGGGTTGGATTTGGATGT+AGG | - | chr7.3:2269115-2269134 | MS.gene050467:CDS | 50.0% | |
| TCAATGCAGCAATGGAGGGT+TGG | - | chr7.3:2269102-2269121 | MS.gene050467:CDS | 50.0% | |
| TGGTACCACACCAACCGTAT+TGG | + | chr7.3:2268590-2268609 | None:intergenic | 50.0% | |
| !!! | ACTGCTTTTTGTGGCCCTGT+TGG | - | chr7.3:2268807-2268826 | MS.gene050467:intron | 50.0% |
| CCACAAGAAGCTTGACCACG+AGG | + | chr7.3:2268834-2268853 | None:intergenic | 55.0% | |
| CCTCGTGGTCAAGCTTCTTG+TGG | - | chr7.3:2268831-2268850 | MS.gene050467:intron | 55.0% | |
| CTTGACCACGAGGTCCAACA+GGG | + | chr7.3:2268824-2268843 | None:intergenic | 55.0% | |
| TGTCATGGCAGTAGTGGTGG+TGG | - | chr7.3:2268645-2268664 | MS.gene050467:CDS | 55.0% | |
| ! | AAATTCAGGGACGGGAGCAC+AGG | - | chr7.3:2269060-2269079 | MS.gene050467:CDS | 55.0% |
| ! | TGCAGCCAATACGGTTGGTG+TGG | - | chr7.3:2268582-2268601 | MS.gene050467:CDS | 55.0% |
| GCTTGACCACGAGGTCCAAC+AGG | + | chr7.3:2268825-2268844 | None:intergenic | 60.0% | |
| TGTGGCCCTGTTGGACCTCG+TGG | - | chr7.3:2268816-2268835 | MS.gene050467:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 2268465 | 2269273 | 2268465 | ID=MS.gene050467 |
| chr7.3 | mRNA | 2268465 | 2269273 | 2268465 | ID=MS.gene050467.t1;Parent=MS.gene050467 |
| chr7.3 | exon | 2268872 | 2269273 | 2268872 | ID=MS.gene050467.t1.exon1;Parent=MS.gene050467.t1 |
| chr7.3 | CDS | 2268872 | 2269273 | 2268872 | ID=cds.MS.gene050467.t1;Parent=MS.gene050467.t1 |
| chr7.3 | exon | 2268465 | 2268683 | 2268465 | ID=MS.gene050467.t1.exon2;Parent=MS.gene050467.t1 |
| chr7.3 | CDS | 2268465 | 2268683 | 2268465 | ID=cds.MS.gene050467.t1;Parent=MS.gene050467.t1 |
| Gene Sequence |
| Protein sequence |
