Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050532.t1 | XP_013450528.1 | 93.7 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 1.70E-112 | 415.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050532.t1 | P02877 | 71.1 | 201 | 51 | 3 | 6 | 202 | 1 | 198 | 5.9e-85 | 315.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050532.t1 | A0A072U5A4 | 93.7 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 1.3e-112 | 415.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050467 | MS.gene050532 | 0.993785 | 3.34E-202 | -1.69E-46 |
| MS.gene050532 | MS.gene053826 | 0.814262 | 1.68E-51 | -1.69E-46 |
| MS.gene050532 | MS.gene060187 | 0.809161 | 2.17E-50 | -1.69E-46 |
| MS.gene050532 | MS.gene23406 | 0.813463 | 2.53E-51 | -1.69E-46 |
| MS.gene050532 | MS.gene36282 | 0.805625 | 1.22E-49 | -1.69E-46 |
| MS.gene050532 | MS.gene61320 | 0.806077 | 9.80E-50 | -1.69E-46 |
| MS.gene050532 | MS.gene63052 | 0.81201 | 5.26E-51 | -1.69E-46 |
| MS.gene050532 | MS.gene89097 | 0.811436 | 7.01E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050532.t1 | MTR_7g115220 | 93.689 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 8.93e-136 | 379 |
| MS.gene050532.t1 | MTR_1g080820 | 71.901 | 121 | 34 | 0 | 68 | 188 | 24 | 144 | 5.76e-64 | 194 |
| MS.gene050532.t1 | MTR_1g080810 | 71.074 | 121 | 35 | 0 | 68 | 188 | 24 | 144 | 2.55e-63 | 193 |
| MS.gene050532.t1 | MTR_1g080780 | 70.000 | 120 | 36 | 0 | 71 | 190 | 22 | 141 | 4.17e-63 | 192 |
| MS.gene050532.t1 | MTR_1g080800 | 70.732 | 123 | 36 | 0 | 68 | 190 | 24 | 146 | 1.18e-62 | 191 |
| MS.gene050532.t1 | MTR_6g083270 | 50.000 | 154 | 60 | 2 | 61 | 197 | 15 | 168 | 1.52e-43 | 151 |
| MS.gene050532.t1 | MTR_6g083200 | 47.692 | 130 | 64 | 2 | 61 | 188 | 15 | 142 | 2.65e-38 | 131 |
| MS.gene050532.t1 | MTR_6g083290 | 47.445 | 137 | 61 | 2 | 61 | 190 | 15 | 147 | 1.60e-34 | 124 |
| MS.gene050532.t1 | MTR_6g083310 | 42.667 | 150 | 69 | 3 | 66 | 200 | 20 | 167 | 1.26e-33 | 118 |
| MS.gene050532.t1 | MTR_3g118390 | 51.515 | 66 | 32 | 0 | 1 | 66 | 59 | 124 | 8.51e-17 | 77.8 |
| MS.gene050532.t1 | MTR_8g074335 | 68.293 | 41 | 13 | 0 | 22 | 62 | 22 | 62 | 7.48e-13 | 66.2 |
| MS.gene050532.t1 | MTR_8g074350 | 50.000 | 56 | 28 | 0 | 6 | 61 | 7 | 62 | 8.66e-12 | 63.2 |
| MS.gene050532.t1 | MTR_8g074330 | 67.500 | 40 | 13 | 0 | 22 | 61 | 22 | 61 | 1.50e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050532.t1 | AT3G04720 | 71.292 | 209 | 52 | 3 | 3 | 203 | 2 | 210 | 3.56e-97 | 281 |
| MS.gene050532.t1 | AT3G12500 | 68.293 | 41 | 12 | 1 | 22 | 61 | 33 | 73 | 8.44e-12 | 63.5 |
Find 59 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTAGACATGGGAAAATT+TGG | 0.218092 | 7.4:-2398384 | None:intergenic |
| ATGATGCAGGTGACAAATTC+AGG | 0.293677 | 7.4:-2397796 | MS.gene050532:intron |
| CAGCAATGGAGGGTTGGATT+TGG | 0.326401 | 7.4:-2397734 | MS.gene050532:CDS |
| TACTCATGGCGTAGCAAGTA+TGG | 0.353266 | 7.4:-2398060 | MS.gene050532:CDS |
| CTTCTTGTGGAAAGTGTCTC+AGG | 0.353594 | 7.4:-2397996 | MS.gene050532:CDS |
| TGGCTGCAACAAAGGTTGTT+TGG | 0.365069 | 7.4:+2398273 | None:intergenic |
| GCTTGACCACGAGGTCCAAC+AGG | 0.397549 | 7.4:+2398018 | None:intergenic |
| GGAGGGTTGGATTTGGATGT+AGG | 0.409363 | 7.4:-2397727 | MS.gene050532:CDS |
| AAGTCCCATCCATGTTGCTC+TGG | 0.422201 | 7.4:+2398129 | None:intergenic |
| TATTGTAAGCTATCAGTTTG+TGG | 0.428774 | 7.4:-2397653 | MS.gene050532:CDS |
| GCTATCAGTTTGTGGATTGT+GGG | 0.462963 | 7.4:-2397645 | MS.gene050532:CDS |
| AGATGGCAGAGGATACCAAC+AGG | 0.473028 | 7.4:-2397683 | MS.gene050532:CDS |
| AACCTTTGTTGCAGCCAATA+CGG | 0.476377 | 7.4:-2398267 | MS.gene050532:CDS |
| TACTTGCTACGCCATGAGTA+AGG | 0.479661 | 7.4:+2398063 | None:intergenic |
| AACCGTATTGGCTGCAACAA+AGG | 0.489204 | 7.4:+2398265 | None:intergenic |
| AAATTCAGGGACGGGAGCAC+AGG | 0.490592 | 7.4:-2397782 | MS.gene050532:CDS |
| AGCTATCAGTTTGTGGATTG+TGG | 0.492406 | 7.4:-2397646 | MS.gene050532:CDS |
| GAACAATGCGGTAAACAAGC+TGG | 0.511319 | 7.4:-2398309 | MS.gene050532:CDS |
| TTATTGTTCAGCATCAATGA+TGG | 0.515481 | 7.4:+2397591 | None:intergenic |
| TGGTACCACACCAACCGTAT+TGG | 0.526780 | 7.4:+2398253 | None:intergenic |
| TAAGTGCTTATTGTTCAACT+TGG | 0.529463 | 7.4:-2398098 | MS.gene050532:CDS |
| TGACAATTACTCTGACAGTT+CGG | 0.538086 | 7.4:+2398213 | None:intergenic |
| CAGGTGACAAATTCAGGGAC+GGG | 0.545443 | 7.4:-2397790 | MS.gene050532:CDS |
| CCTCGTGGTCAAGCTTCTTG+TGG | 0.556286 | 7.4:-2398009 | MS.gene050532:CDS |
| TCAATGCAGCAATGGAGGGT+TGG | 0.558488 | 7.4:-2397740 | MS.gene050532:CDS |
| AAGTGCTTATTGTTCAACTT+GGG | 0.564174 | 7.4:-2398097 | MS.gene050532:CDS |
| AATGATGGAGAACAGAGGAT+TGG | 0.564526 | 7.4:+2397606 | None:intergenic |
| GTAGATCAATGCAGCAATGG+AGG | 0.578522 | 7.4:-2397745 | MS.gene050532:CDS |
| TTTGTTGCAGCCAATACGGT+TGG | 0.578744 | 7.4:-2398263 | MS.gene050532:CDS |
| TGATGCAGGTGACAAATTCA+GGG | 0.583955 | 7.4:-2397795 | MS.gene050532:intron |
| ATGCAAGCAAACCTTACTCA+TGG | 0.586811 | 7.4:-2398074 | MS.gene050532:CDS |
| ATCAACCAGAGCAACATGGA+TGG | 0.587569 | 7.4:-2398134 | MS.gene050532:CDS |
| TCAACCAGAGCAACATGGAT+GGG | 0.598724 | 7.4:-2398133 | MS.gene050532:CDS |
| CATGGCGTAGCAAGTATGGT+TGG | 0.600676 | 7.4:-2398056 | MS.gene050532:CDS |
| TAGATCAATGCAGCAATGGA+GGG | 0.603290 | 7.4:-2397744 | MS.gene050532:CDS |
| TTACAATAAGATGTCCCTGT+TGG | 0.604159 | 7.4:+2397668 | None:intergenic |
| GCAGGTGACAAATTCAGGGA+CGG | 0.605105 | 7.4:-2397791 | MS.gene050532:intron |
| AGTAATTGTCATGGCAGTAG+TGG | 0.614410 | 7.4:-2398201 | MS.gene050532:CDS |
| TGTGGCCCTGTTGGACCTCG+TGG | 0.621769 | 7.4:-2398024 | MS.gene050532:CDS |
| TGTCATGGCAGTAGTGGTGG+TGG | 0.621989 | 7.4:-2398195 | MS.gene050532:CDS |
| TTCTTGTGGAAAGTGTCTCA+GGG | 0.622227 | 7.4:-2397995 | MS.gene050532:intron |
| AATTGTCATGGCAGTAGTGG+TGG | 0.624496 | 7.4:-2398198 | MS.gene050532:CDS |
| AAGCCTAGCGGTATTAACAT+TGG | 0.627981 | 7.4:-2398358 | MS.gene050532:CDS |
| AACTGTCAGAGTAATTGTCA+TGG | 0.629228 | 7.4:-2398210 | MS.gene050532:CDS |
| ATTGTAGATCAATGCAGCAA+TGG | 0.636888 | 7.4:-2397748 | MS.gene050532:CDS |
| TACTATCAACCAGAGCAACA+TGG | 0.648851 | 7.4:-2398138 | MS.gene050532:CDS |
| TGCAGCCAATACGGTTGGTG+TGG | 0.651269 | 7.4:-2398258 | MS.gene050532:CDS |
| TTTAACAGAATCGACACAGA+TGG | 0.651956 | 7.4:-2397700 | MS.gene050532:CDS |
| CCACAAGAAGCTTGACCACG+AGG | 0.656609 | 7.4:+2398009 | None:intergenic |
| AGAATCGACACAGATGGCAG+AGG | 0.657142 | 7.4:-2397694 | MS.gene050532:CDS |
| ACACCAATGTTAATACCGCT+AGG | 0.661884 | 7.4:+2398355 | None:intergenic |
| CAATGCGGTAAACAAGCTGG+AGG | 0.695826 | 7.4:-2398306 | MS.gene050532:CDS |
| CTTGACCACGAGGTCCAACA+GGG | 0.697195 | 7.4:+2398019 | None:intergenic |
| CGGACCACAGTATTCATCTG+TGG | 0.698610 | 7.4:+2398233 | None:intergenic |
| GCATCAATGATGGAGAACAG+AGG | 0.711827 | 7.4:+2397601 | None:intergenic |
| GATGGCAGAGGATACCAACA+GGG | 0.745988 | 7.4:-2397682 | MS.gene050532:CDS |
| ACAACAGTTGCAGAACAATG+CGG | 0.746680 | 7.4:-2398321 | MS.gene050532:CDS |
| AAAATTTGGAGTAAGCCTAG+CGG | 0.781644 | 7.4:-2398370 | MS.gene050532:CDS |
| GGTACCACAGATGAATACTG+TGG | 0.812966 | 7.4:-2398237 | MS.gene050532:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGAGTAAATATAGATAAAA+AGG | + | chr7.4:2398028-2398047 | None:intergenic | 15.0% |
| !! | TAGATAAAAAGGAATATAAT+TGG | + | chr7.4:2398017-2398036 | None:intergenic | 15.0% |
| !!! | TACTCTAATGTTTTAAAAAT+AGG | - | chr7.4:2398041-2398060 | MS.gene050532:CDS | 15.0% |
| ! | CAATTGAACTATATATGATC+TGG | + | chr7.4:2398084-2398103 | None:intergenic | 25.0% |
| AAGTGCTTATTGTTCAACTT+GGG | - | chr7.4:2397869-2397888 | MS.gene050532:intron | 30.0% | |
| TAAGTGCTTATTGTTCAACT+TGG | - | chr7.4:2397868-2397887 | MS.gene050532:intron | 30.0% | |
| TATTGTAAGCTATCAGTTTG+TGG | - | chr7.4:2398313-2398332 | MS.gene050532:CDS | 30.0% | |
| TTATTGTTCAGCATCAATGA+TGG | + | chr7.4:2398378-2398397 | None:intergenic | 30.0% | |
| AACTGTCAGAGTAATTGTCA+TGG | - | chr7.4:2397756-2397775 | MS.gene050532:CDS | 35.0% | |
| ATTGTAGATCAATGCAGCAA+TGG | - | chr7.4:2398218-2398237 | MS.gene050532:CDS | 35.0% | |
| TGACAATTACTCTGACAGTT+CGG | + | chr7.4:2397756-2397775 | None:intergenic | 35.0% | |
| TTACAATAAGATGTCCCTGT+TGG | + | chr7.4:2398301-2398320 | None:intergenic | 35.0% | |
| TTTAACAGAATCGACACAGA+TGG | - | chr7.4:2398266-2398285 | MS.gene050532:CDS | 35.0% | |
| ! | AAAATTTGGAGTAAGCCTAG+CGG | - | chr7.4:2397596-2397615 | MS.gene050532:CDS | 35.0% |
| ! | GAATGTAAATGTGATGATGC+AGG | - | chr7.4:2398157-2398176 | MS.gene050532:CDS | 35.0% |
| AACCTTTGTTGCAGCCAATA+CGG | - | chr7.4:2397699-2397718 | MS.gene050532:CDS | 40.0% | |
| AAGCCTAGCGGTATTAACAT+TGG | - | chr7.4:2397608-2397627 | MS.gene050532:CDS | 40.0% | |
| ACAACAGTTGCAGAACAATG+CGG | - | chr7.4:2397645-2397664 | MS.gene050532:CDS | 40.0% | |
| ACACCAATGTTAATACCGCT+AGG | + | chr7.4:2397614-2397633 | None:intergenic | 40.0% | |
| AGTAATTGTCATGGCAGTAG+TGG | - | chr7.4:2397765-2397784 | MS.gene050532:CDS | 40.0% | |
| ATGATGCAGGTGACAAATTC+AGG | - | chr7.4:2398170-2398189 | MS.gene050532:CDS | 40.0% | |
| ATGCAAGCAAACCTTACTCA+TGG | - | chr7.4:2397892-2397911 | MS.gene050532:intron | 40.0% | |
| TACTATCAACCAGAGCAACA+TGG | - | chr7.4:2397828-2397847 | MS.gene050532:intron | 40.0% | |
| TAGATCAATGCAGCAATGGA+GGG | - | chr7.4:2398222-2398241 | MS.gene050532:CDS | 40.0% | |
| TGATGCAGGTGACAAATTCA+GGG | - | chr7.4:2398171-2398190 | MS.gene050532:CDS | 40.0% | |
| ! | AATGATGGAGAACAGAGGAT+TGG | + | chr7.4:2398363-2398382 | None:intergenic | 40.0% |
| ! | AGCTATCAGTTTGTGGATTG+TGG | - | chr7.4:2398320-2398339 | MS.gene050532:CDS | 40.0% |
| ! | GCTATCAGTTTGTGGATTGT+GGG | - | chr7.4:2398321-2398340 | MS.gene050532:CDS | 40.0% |
| !! | TTCTTGTGGAAAGTGTCTCA+GGG | - | chr7.4:2397971-2397990 | MS.gene050532:intron | 40.0% |
| !!! | TATGGTTGGACTGCTTTTTG+TGG | - | chr7.4:2397924-2397943 | MS.gene050532:intron | 40.0% |
| AATTGTCATGGCAGTAGTGG+TGG | - | chr7.4:2397768-2397787 | MS.gene050532:CDS | 45.0% | |
| ATCAACCAGAGCAACATGGA+TGG | - | chr7.4:2397832-2397851 | MS.gene050532:intron | 45.0% | |
| GAACAATGCGGTAAACAAGC+TGG | - | chr7.4:2397657-2397676 | MS.gene050532:CDS | 45.0% | |
| GGTACCACAGATGAATACTG+TGG | - | chr7.4:2397729-2397748 | MS.gene050532:CDS | 45.0% | |
| GTAGATCAATGCAGCAATGG+AGG | - | chr7.4:2398221-2398240 | MS.gene050532:CDS | 45.0% | |
| TACTCATGGCGTAGCAAGTA+TGG | - | chr7.4:2397906-2397925 | MS.gene050532:intron | 45.0% | |
| TCAACCAGAGCAACATGGAT+GGG | - | chr7.4:2397833-2397852 | MS.gene050532:intron | 45.0% | |
| TGGCTGCAACAAAGGTTGTT+TGG | + | chr7.4:2397696-2397715 | None:intergenic | 45.0% | |
| TTTGTTGCAGCCAATACGGT+TGG | - | chr7.4:2397703-2397722 | MS.gene050532:CDS | 45.0% | |
| ! | GCATCAATGATGGAGAACAG+AGG | + | chr7.4:2398368-2398387 | None:intergenic | 45.0% |
| ! | TACTTGCTACGCCATGAGTA+AGG | + | chr7.4:2397906-2397925 | None:intergenic | 45.0% |
| !! | AACCGTATTGGCTGCAACAA+AGG | + | chr7.4:2397704-2397723 | None:intergenic | 45.0% |
| !! | CTTCTTGTGGAAAGTGTCTC+AGG | - | chr7.4:2397970-2397989 | MS.gene050532:intron | 45.0% |
| AAGTCCCATCCATGTTGCTC+TGG | + | chr7.4:2397840-2397859 | None:intergenic | 50.0% | |
| AGAATCGACACAGATGGCAG+AGG | - | chr7.4:2398272-2398291 | MS.gene050532:CDS | 50.0% | |
| AGATGGCAGAGGATACCAAC+AGG | - | chr7.4:2398283-2398302 | MS.gene050532:CDS | 50.0% | |
| CAATGCGGTAAACAAGCTGG+AGG | - | chr7.4:2397660-2397679 | MS.gene050532:CDS | 50.0% | |
| CAGCAATGGAGGGTTGGATT+TGG | - | chr7.4:2398232-2398251 | MS.gene050532:CDS | 50.0% | |
| CAGGTGACAAATTCAGGGAC+GGG | - | chr7.4:2398176-2398195 | MS.gene050532:CDS | 50.0% | |
| CATGGCGTAGCAAGTATGGT+TGG | - | chr7.4:2397910-2397929 | MS.gene050532:intron | 50.0% | |
| CGGACCACAGTATTCATCTG+TGG | + | chr7.4:2397736-2397755 | None:intergenic | 50.0% | |
| GATGGCAGAGGATACCAACA+GGG | - | chr7.4:2398284-2398303 | MS.gene050532:CDS | 50.0% | |
| GCAGGTGACAAATTCAGGGA+CGG | - | chr7.4:2398175-2398194 | MS.gene050532:CDS | 50.0% | |
| GGAGGGTTGGATTTGGATGT+AGG | - | chr7.4:2398239-2398258 | MS.gene050532:CDS | 50.0% | |
| TCAATGCAGCAATGGAGGGT+TGG | - | chr7.4:2398226-2398245 | MS.gene050532:CDS | 50.0% | |
| TGGTACCACACCAACCGTAT+TGG | + | chr7.4:2397716-2397735 | None:intergenic | 50.0% | |
| !!! | ACTGCTTTTTGTGGCCCTGT+TGG | - | chr7.4:2397933-2397952 | MS.gene050532:intron | 50.0% |
| CCACAAGAAGCTTGACCACG+AGG | + | chr7.4:2397960-2397979 | None:intergenic | 55.0% | |
| CCTCGTGGTCAAGCTTCTTG+TGG | - | chr7.4:2397957-2397976 | MS.gene050532:intron | 55.0% | |
| CTTGACCACGAGGTCCAACA+GGG | + | chr7.4:2397950-2397969 | None:intergenic | 55.0% | |
| TGTCATGGCAGTAGTGGTGG+TGG | - | chr7.4:2397771-2397790 | MS.gene050532:CDS | 55.0% | |
| ! | AAATTCAGGGACGGGAGCAC+AGG | - | chr7.4:2398184-2398203 | MS.gene050532:CDS | 55.0% |
| ! | TGCAGCCAATACGGTTGGTG+TGG | - | chr7.4:2397708-2397727 | MS.gene050532:CDS | 55.0% |
| GCTTGACCACGAGGTCCAAC+AGG | + | chr7.4:2397951-2397970 | None:intergenic | 60.0% | |
| TGTGGCCCTGTTGGACCTCG+TGG | - | chr7.4:2397942-2397961 | MS.gene050532:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 2397591 | 2398397 | 2397591 | ID=MS.gene050532 |
| chr7.4 | mRNA | 2397591 | 2398397 | 2397591 | ID=MS.gene050532.t1;Parent=MS.gene050532 |
| chr7.4 | exon | 2397996 | 2398397 | 2397996 | ID=MS.gene050532.t1.exon1;Parent=MS.gene050532.t1 |
| chr7.4 | CDS | 2397996 | 2398397 | 2397996 | ID=cds.MS.gene050532.t1;Parent=MS.gene050532.t1 |
| chr7.4 | exon | 2397591 | 2397809 | 2397591 | ID=MS.gene050532.t1.exon2;Parent=MS.gene050532.t1 |
| chr7.4 | CDS | 2397591 | 2397809 | 2397591 | ID=cds.MS.gene050532.t1;Parent=MS.gene050532.t1 |
| Gene Sequence |
| Protein sequence |
