AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050695


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050695.t1 MTR_7g118230 70.166 181 29 3 60 240 33 188 3.17e-72 224
MS.gene050695.t1 MTR_1g056520 33.133 166 87 3 55 213 101 249 8.92e-20 86.7
MS.gene050695.t1 MTR_1g045510 32.000 175 100 2 70 235 113 277 1.11e-18 83.2
MS.gene050695.t1 MTR_7g106440 30.189 159 109 1 57 213 96 254 1.50e-18 83.2
MS.gene050695.t1 MTR_3g027140 33.333 132 86 1 71 202 61 190 2.96e-18 81.3
MS.gene050695.t1 MTR_1g097440 33.562 146 84 3 62 207 113 245 1.78e-14 72.8
MS.gene050695.t1 MTR_4g104470 41.573 89 50 1 60 148 208 294 7.88e-14 69.7
MS.gene050695.t1 MTR_4g104470 27.222 180 115 3 71 241 123 295 1.08e-13 69.3
MS.gene050695.t1 MTR_3g075500 28.409 176 109 3 71 235 110 279 3.01e-13 68.2
MS.gene050695.t1 MTR_3g075500 37.778 90 52 2 55 143 193 279 4.87e-12 64.7
MS.gene050695.t1 MTR_3g075500 30.556 144 92 2 71 212 110 247 4.43e-13 67.4
MS.gene050695.t1 MTR_1g064230 27.559 254 138 6 9 233 21 257 2.27e-12 65.5
MS.gene050695.t1 MTR_6g040190 31.579 133 77 3 72 204 115 233 4.29e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050695.t1 AT1G01080 49.231 195 82 4 64 244 102 293 2.63e-57 184
MS.gene050695.t1 AT1G01080 48.980 196 82 5 64 244 102 294 1.47e-55 180
MS.gene050695.t1 AT1G01080 51.497 167 73 3 64 225 102 265 6.62e-51 167
MS.gene050695.t1 AT3G52150 31.667 180 105 4 37 202 33 208 4.49e-19 84.0
MS.gene050695.t1 AT3G52150 31.667 180 105 4 37 202 33 208 4.49e-19 84.0
MS.gene050695.t1 AT3G52380 29.545 176 118 3 67 240 113 284 1.15e-18 84.0
MS.gene050695.t1 AT1G60000 28.253 269 140 10 1 235 1 250 2.47e-16 76.3
MS.gene050695.t1 AT5G50250 30.968 155 94 3 59 213 108 249 1.98e-15 74.3
MS.gene050695.t1 AT5G50250 38.462 91 54 1 58 148 197 285 5.78e-13 67.4
MS.gene050695.t1 AT2G37220 26.857 175 96 2 61 213 82 246 4.30e-14 70.5
MS.gene050695.t1 AT4G24770 32.168 143 90 2 71 213 151 286 3.72e-13 68.2
MS.gene050695.t1 AT4G24770 41.026 78 46 0 71 148 245 322 8.10e-13 67.4
MS.gene050695.t1 AT4G24770 32.168 143 90 2 71 213 151 286 3.72e-13 68.2
MS.gene050695.t1 AT4G24770 41.026 78 46 0 71 148 245 322 8.10e-13 67.4
MS.gene050695.t1 AT3G16380 31.655 139 85 2 72 210 23 151 5.61e-12 65.5
MS.gene050695.t1 AT1G22760 27.907 172 113 3 34 205 103 263 2.38e-11 63.5

Find 60 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATCTCTTTGGTAAATT+TGG 0.099306 7.4:-429431 MS.gene050695:CDS
AAGAGATGTCTCACGTCTTC+AGG 0.315903 7.4:+429443 None:intergenic
TCTCACCAGTCTCTGCATTT+CGG 0.377067 7.4:+429771 None:intergenic
TGTCGAGTATCACAGCTTCT+AGG 0.377323 7.4:+429914 None:intergenic
CAGAGACTGGTGAGAGTAAA+GGG 0.393341 7.4:-429763 MS.gene050695:CDS
CTCCAAGTAGTGGCTGCTTA+TGG 0.394918 7.4:+430086 None:intergenic
GGTGTTGCAAGATTTGAATC+AGG 0.395551 7.4:-429390 MS.gene050695:CDS
TACCATAAGCAGCCACTACT+TGG 0.428423 7.4:-430088 MS.gene050695:CDS
TGCAGACGTAAACTTGGGTT+GGG 0.442375 7.4:+429942 None:intergenic
TAACGAAGAGGAAGAGAAAG+AGG 0.445652 7.4:+430114 None:intergenic
TTCCCAACATACACCTTATG+AGG 0.456223 7.4:+429485 None:intergenic
GAAGGTTGCAGACGTAAACT+TGG 0.462333 7.4:+429936 None:intergenic
TGTTGGTGGGCGCGAAATGC+GGG 0.472414 7.4:-429588 MS.gene050695:CDS
CTTTGGTAAATTTGGAAATG+TGG 0.479793 7.4:-429423 MS.gene050695:CDS
GCAGAGACTGGTGAGAGTAA+AGG 0.480321 7.4:-429764 MS.gene050695:CDS
GTCCTCATAAGGTGTATGTT+GGG 0.485859 7.4:-429487 MS.gene050695:CDS
TTGCAGACGTAAACTTGGGT+TGG 0.485972 7.4:+429941 None:intergenic
ATACTATGAAGGTCCTCATA+AGG 0.498833 7.4:-429498 MS.gene050695:CDS
GAAGACGTGAGACATCTCTT+TGG 0.500754 7.4:-429440 MS.gene050695:CDS
GGGAGTGCTTATGTCACAAT+GGG 0.508546 7.4:-429743 MS.gene050695:CDS
TCGTTTGAATTGAAGGATGT+TGG 0.510713 7.4:-429605 MS.gene050695:intron
ACAACCACGTAAGTTGAAGC+TGG 0.512125 7.4:-429969 MS.gene050695:CDS
CGCGAGGAGAAAGAAGGATA+AGG 0.512495 7.4:+430025 None:intergenic
AATGCACTTGCTGCATTGGA+TGG 0.517474 7.4:-429701 MS.gene050695:CDS
GGTCCTCATAAGGTGTATGT+TGG 0.523656 7.4:-429488 MS.gene050695:CDS
GAAACACAGAGTAGCAGAGT+TGG 0.527590 7.4:+430138 None:intergenic
GTATATAGTTCTTCTTCTCA+CGG 0.533646 7.4:+429517 None:intergenic
TCTAGTGGTTAGAGAGGGTG+TGG 0.535491 7.4:-429191 MS.gene050695:CDS
AGAAGAACTATATACTATGA+AGG 0.536527 7.4:-429509 MS.gene050695:CDS
ATGTTGGTGGGCGCGAAATG+CGG 0.539679 7.4:-429589 MS.gene050695:CDS
TATATAGTTCTTCTTCTCAC+GGG 0.541659 7.4:+429518 None:intergenic
GTGTTGCAAGATTTGAATCA+GGG 0.546447 7.4:-429389 MS.gene050695:CDS
TCGAACTCTAGTGGTTAGAG+AGG 0.547494 7.4:-429197 MS.gene050695:CDS
CAACCACGTAAGTTGAAGCT+GGG 0.548485 7.4:-429968 MS.gene050695:CDS
AAGCAGCCACTACTTGGAGT+TGG 0.552480 7.4:-430082 MS.gene050695:CDS
GAGGAGTGAAGAGATGAAGT+AGG 0.557135 7.4:+429891 None:intergenic
CTGTGACGCGAGGAGAAAGA+AGG 0.561006 7.4:+430019 None:intergenic
TTTGAATTGAAGGATGTTGG+TGG 0.563904 7.4:-429602 MS.gene050695:intron
GTTTGCCGAAATGCAGAGAC+TGG 0.571702 7.4:-429776 MS.gene050695:CDS
TTGAATTGAAGGATGTTGGT+GGG 0.579799 7.4:-429601 MS.gene050695:intron
GAGTATCCAACTCCAAGTAG+TGG 0.587862 7.4:+430076 None:intergenic
CTTCATCTCTTCACTCCTCA+CGG 0.594839 7.4:-429887 MS.gene050695:CDS
TTCCCCAGCTTCAACTTACG+TGG 0.601446 7.4:+429965 None:intergenic
AGGGAGTGCTTATGTCACAA+TGG 0.612091 7.4:-429744 MS.gene050695:CDS
AAGGTTGCAGACGTAAACTT+GGG 0.613456 7.4:+429937 None:intergenic
TTGAGAGAGATAAGAGACAA+AGG 0.615725 7.4:+429344 None:intergenic
AATGGACGCGCTGTGACGCG+AGG 0.621619 7.4:+430009 None:intergenic
ACTTGCTGCATTGGATGGAT+CGG 0.623524 7.4:-429696 MS.gene050695:intron
GAGTATCACAGCTTCTAGGA+AGG 0.626004 7.4:+429918 None:intergenic
TGGAAATGTGGCTAGTGTGA+GGG 0.626364 7.4:-429411 MS.gene050695:CDS
ATAGTTCTTCTTCTCACGGG+AGG 0.629773 7.4:+429521 None:intergenic
TTGGAAATGTGGCTAGTGTG+AGG 0.645229 7.4:-429412 MS.gene050695:CDS
ACGTACCTCGGTAGATAGAA+CGG 0.653839 7.4:+429862 None:intergenic
CGAACTCTAGTGGTTAGAGA+GGG 0.654487 7.4:-429196 MS.gene050695:CDS
TGTTGCAAGATTTGAATCAG+GGG 0.656854 7.4:-429388 MS.gene050695:CDS
AACCACGTAAGTTGAAGCTG+GGG 0.663989 7.4:-429967 MS.gene050695:CDS
CTTATGGTAGAGTAACGAAG+AGG 0.714741 7.4:+430102 None:intergenic
AAGAGACAAAGGCATAGACA+CGG 0.730141 7.4:+429355 None:intergenic
GTAGATAGAACGGTACCGTG+AGG 0.731002 7.4:+429872 None:intergenic
CGGTACCGTTCTATCTACCG+AGG 0.742490 7.4:-429867 MS.gene050695:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTATTACTTGTAATGTAA+TGG - chr7.4:429497-429516 MS.gene050695:CDS 20.0%
!!! ATATTTTACAGTTTCATTGA+GGG + chr7.4:430023-430042 None:intergenic 20.0%
! AGAAGAACTATATACTATGA+AGG - chr7.4:429810-429829 MS.gene050695:intron 25.0%
!! CATATTTTACAGTTTCATTG+AGG + chr7.4:430024-430043 None:intergenic 25.0%
!!! TTGTTTTTCGTTTGAATTGA+AGG - chr7.4:429707-429726 MS.gene050695:CDS 25.0%
AGACATCTCTTTGGTAAATT+TGG - chr7.4:429888-429907 MS.gene050695:CDS 30.0%
CTTTGGTAAATTTGGAAATG+TGG - chr7.4:429896-429915 MS.gene050695:CDS 30.0%
GCAGTAGAAAAAGTGAAAAA+AGG + chr7.4:430053-430072 None:intergenic 30.0%
GTAGAAAAAGTGAAAAAAGG+TGG + chr7.4:430050-430069 None:intergenic 30.0%
GTATATAGTTCTTCTTCTCA+CGG + chr7.4:429805-429824 None:intergenic 30.0%
TATATAGTTCTTCTTCTCAC+GGG + chr7.4:429804-429823 None:intergenic 30.0%
! TTCTTCTTCGTTTTCTACAA+TGG + chr7.4:429331-429350 None:intergenic 30.0%
ATACTATGAAGGTCCTCATA+AGG - chr7.4:429821-429840 MS.gene050695:intron 35.0%
ATCTTATAATGCTGTGCTGT+AGG - chr7.4:429521-429540 MS.gene050695:CDS 35.0%
GAAAAAGTGAAAAAAGGTGG+AGG + chr7.4:430047-430066 None:intergenic 35.0%
GTGTTGCAAGATTTGAATCA+GGG - chr7.4:429930-429949 MS.gene050695:CDS 35.0%
TCGTTTGAATTGAAGGATGT+TGG - chr7.4:429714-429733 MS.gene050695:CDS 35.0%
TGTTGCAAGATTTGAATCAG+GGG - chr7.4:429931-429950 MS.gene050695:CDS 35.0%
TTGAATTGAAGGATGTTGGT+GGG - chr7.4:429718-429737 MS.gene050695:CDS 35.0%
TTGAGAGAGATAAGAGACAA+AGG + chr7.4:429978-429997 None:intergenic 35.0%
TTTGAATTGAAGGATGTTGG+TGG - chr7.4:429717-429736 MS.gene050695:CDS 35.0%
! TAAAAATGCACTTGCTGCAT+TGG - chr7.4:429614-429633 MS.gene050695:intron 35.0%
! TTGTTCTTGCAGCAATTTTG+TGG - chr7.4:430099-430118 MS.gene050695:CDS 35.0%
AAGGTTGCAGACGTAAACTT+GGG + chr7.4:429385-429404 None:intergenic 40.0%
AGCAACATACTTACGTACCT+CGG + chr7.4:429472-429491 None:intergenic 40.0%
AGTTTCATTGAGGGACAAAG+AGG + chr7.4:430014-430033 None:intergenic 40.0%
CTTATGGTAGAGTAACGAAG+AGG + chr7.4:429220-429239 None:intergenic 40.0%
GTCCTCATAAGGTGTATGTT+GGG - chr7.4:429832-429851 MS.gene050695:intron 40.0%
TAACGAAGAGGAAGAGAAAG+AGG + chr7.4:429208-429227 None:intergenic 40.0%
TTCCCAACATACACCTTATG+AGG + chr7.4:429837-429856 None:intergenic 40.0%
! AAGAGACAAAGGCATAGACA+CGG + chr7.4:429967-429986 None:intergenic 40.0%
! ATTTTGTGGTCGAACTCTAG+TGG - chr7.4:430113-430132 MS.gene050695:CDS 40.0%
! GGTGTTGCAAGATTTGAATC+AGG - chr7.4:429929-429948 MS.gene050695:CDS 40.0%
AAGAGATGTCTCACGTCTTC+AGG + chr7.4:429879-429898 None:intergenic 45.0%
ACGTACCTCGGTAGATAGAA+CGG + chr7.4:429460-429479 None:intergenic 45.0%
ATAGTTCTTCTTCTCACGGG+AGG + chr7.4:429801-429820 None:intergenic 45.0%
CAGAGACTGGTGAGAGTAAA+GGG - chr7.4:429556-429575 MS.gene050695:CDS 45.0%
CGAACTCTAGTGGTTAGAGA+GGG - chr7.4:430123-430142 MS.gene050695:CDS 45.0%
CTTCATCTCTTCACTCCTCA+CGG - chr7.4:429432-429451 MS.gene050695:CDS 45.0%
GAAACACAGAGTAGCAGAGT+TGG + chr7.4:429184-429203 None:intergenic 45.0%
GAAGACGTGAGACATCTCTT+TGG - chr7.4:429879-429898 MS.gene050695:CDS 45.0%
GAAGGTTGCAGACGTAAACT+TGG + chr7.4:429386-429405 None:intergenic 45.0%
GAGGAGTGAAGAGATGAAGT+AGG + chr7.4:429431-429450 None:intergenic 45.0%
GAGTATCCAACTCCAAGTAG+TGG + chr7.4:429246-429265 None:intergenic 45.0%
GCTGCAAGAACAAGACTAGT+AGG + chr7.4:430092-430111 None:intergenic 45.0%
GGTCCTCATAAGGTGTATGT+TGG - chr7.4:429831-429850 MS.gene050695:intron 45.0%
TACCATAAGCAGCCACTACT+TGG - chr7.4:429231-429250 MS.gene050695:intron 45.0%
TCGAACTCTAGTGGTTAGAG+AGG - chr7.4:430122-430141 MS.gene050695:CDS 45.0%
TCTCACCAGTCTCTGCATTT+CGG + chr7.4:429551-429570 None:intergenic 45.0%
TCTCTCTCAAAGAGAACGTG+AGG - chr7.4:429988-430007 MS.gene050695:CDS 45.0%
TGCAGACGTAAACTTGGGTT+GGG + chr7.4:429380-429399 None:intergenic 45.0%
TGGAAATGTGGCTAGTGTGA+GGG - chr7.4:429908-429927 MS.gene050695:CDS 45.0%
TGTCGAGTATCACAGCTTCT+AGG + chr7.4:429408-429427 None:intergenic 45.0%
TTGCAGACGTAAACTTGGGT+TGG + chr7.4:429381-429400 None:intergenic 45.0%
TTGGAAATGTGGCTAGTGTG+AGG - chr7.4:429907-429926 MS.gene050695:CDS 45.0%
! AACCACGTAAGTTGAAGCTG+GGG - chr7.4:429352-429371 MS.gene050695:CDS 45.0%
! AATGCACTTGCTGCATTGGA+TGG - chr7.4:429618-429637 MS.gene050695:intron 45.0%
! ACAACCACGTAAGTTGAAGC+TGG - chr7.4:429350-429369 MS.gene050695:CDS 45.0%
! ACTTGCTGCATTGGATGGAT+CGG - chr7.4:429623-429642 MS.gene050695:intron 45.0%
! CAACCACGTAAGTTGAAGCT+GGG - chr7.4:429351-429370 MS.gene050695:CDS 45.0%
! GAGTATCACAGCTTCTAGGA+AGG + chr7.4:429404-429423 None:intergenic 45.0%
!! AGGGAGTGCTTATGTCACAA+TGG - chr7.4:429575-429594 MS.gene050695:CDS 45.0%
!! GGGAGTGCTTATGTCACAAT+GGG - chr7.4:429576-429595 MS.gene050695:CDS 45.0%
AAGCAGCCACTACTTGGAGT+TGG - chr7.4:429237-429256 MS.gene050695:intron 50.0%
CGCGAGGAGAAAGAAGGATA+AGG + chr7.4:429297-429316 None:intergenic 50.0%
CTCCAAGTAGTGGCTGCTTA+TGG + chr7.4:429236-429255 None:intergenic 50.0%
GCAGAGACTGGTGAGAGTAA+AGG - chr7.4:429555-429574 MS.gene050695:CDS 50.0%
GTAGATAGAACGGTACCGTG+AGG + chr7.4:429450-429469 None:intergenic 50.0%
GTTTGCCGAAATGCAGAGAC+TGG - chr7.4:429543-429562 MS.gene050695:CDS 50.0%
TCTAGTGGTTAGAGAGGGTG+TGG - chr7.4:430128-430147 MS.gene050695:CDS 50.0%
TTCCCCAGCTTCAACTTACG+TGG + chr7.4:429357-429376 None:intergenic 50.0%
CGGTACCGTTCTATCTACCG+AGG - chr7.4:429452-429471 MS.gene050695:CDS 55.0%
CTGTGACGCGAGGAGAAAGA+AGG + chr7.4:429303-429322 None:intergenic 55.0%
! ATGTTGGTGGGCGCGAAATG+CGG - chr7.4:429730-429749 MS.gene050695:CDS 55.0%
! TGTTGGTGGGCGCGAAATGC+GGG - chr7.4:429731-429750 MS.gene050695:CDS 60.0%
AATGGACGCGCTGTGACGCG+AGG + chr7.4:429313-429332 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 429174 430167 429174 ID=MS.gene050695
chr7.4 mRNA 429174 430167 429174 ID=MS.gene050695.t1;Parent=MS.gene050695
chr7.4 exon 429868 430167 429868 ID=MS.gene050695.t1.exon1;Parent=MS.gene050695.t1
chr7.4 CDS 429868 430167 429868 ID=cds.MS.gene050695.t1;Parent=MS.gene050695.t1
chr7.4 exon 429697 429798 429697 ID=MS.gene050695.t1.exon2;Parent=MS.gene050695.t1
chr7.4 CDS 429697 429798 429697 ID=cds.MS.gene050695.t1;Parent=MS.gene050695.t1
chr7.4 exon 429337 429612 429337 ID=MS.gene050695.t1.exon3;Parent=MS.gene050695.t1
chr7.4 CDS 429337 429612 429337 ID=cds.MS.gene050695.t1;Parent=MS.gene050695.t1
chr7.4 exon 429174 429230 429174 ID=MS.gene050695.t1.exon4;Parent=MS.gene050695.t1
chr7.4 CDS 429174 429230 429174 ID=cds.MS.gene050695.t1;Parent=MS.gene050695.t1
Gene Sequence

>MS.gene050695

ATGGCTGCCAACTCTGCTACTCTGTGTTTCTCCTCTTTCTCTTCCTCTTCGTTACTCTACCATAAGCAGCCACTACTTGGAGTTGGATACTCAAAACGATTCGTTTCGTTTTCTGCGCTGCCTTATCCTTCTTTCTCCTCGCGTCACAGCGCGTCCATTGTAGAAAACGAAGAAGAACAACCACGTAAGTTGAAGCTGGGGAACCCAACCCAAGTTTACGTCTGCAACCTTCCTAGAAGCTGTGATACTCGACACCTACTTCATCTCTTCACTCCTCACGGTACCGTTCTATCTACCGAGGTACGTAAGTATGTTGCTTTCTTCATTATTACTTGTAATGTAATGGAATCTTATAATGCTGTGCTGTAGGTTTGCCGAAATGCAGAGACTGGTGAGAGTAAAGGGAGTGCTTATGTCACAATGGGATCTTATAATGCTGCTAAAAATGCACTTGCTGCATTGGATGGATCGGTTAGTCACTATCATATCTAATAAATGCACTTGCTGCAATGCATGATCTGAATAATGAAACTTTGTTTTTCGTTTGAATTGAAGGATGTTGGTGGGCGCGAAATGCGGGTTAAATTTTCTGTTGAGATGAATAATTTAGAGACATTGAACTCACCTCCCGTGAGAAGAAGAACTATATACTATGAAGGTCCTCATAAGGTGTATGTTGGGAATCTCGCTAAGTCTGCTACACCTGAAGACGTGAGACATCTCTTTGGTAAATTTGGAAATGTGGCTAGTGTGAGGGTGTTGCAAGATTTGAATCAGGGGAAAAAAAAAGCCGTGTCTATGCCTTTGTCTCTTATCTCTCTCAAAGAGAACGTGAGGCCTCTTTGTCCCTCAATGAAACTGTAAAATATGCCTCCACCTTTTTTCACTTTTTCTACTGCTTATTTCTATCTCATGCCTACTAGTCTTGTTCTTGCAGCAATTTTGTGGTCGAACTCTAGTGGTTAGAGAGGGTGTGGAGAAGAATGAGACCTAA

Protein sequence

>MS.gene050695.t1

MAANSATLCFSSFSSSSLLYHKQPLLGVGYSKRFVSFSALPYPSFSSRHSASIVENEEEQPRKLKLGNPTQVYVCNLPRSCDTRHLLHLFTPHGTVLSTEVCRNAETGESKGSAYVTMGSYNAAKNALAALDGSDVGGREMRVKFSVEMNNLETLNSPPVRRRTIYYEGPHKVYVGNLAKSATPEDVRHLFGKFGNVASVRVLQDLNQGKKKAVSMPLSLISLKENQFCGRTLVVREGVEKNET