Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053405.t1 | XP_003590040.1 | 99.1 | 322 | 3 | 0 | 1 | 322 | 1 | 322 | 2.50E-190 | 674.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053405.t1 | P48489 | 83.3 | 299 | 49 | 1 | 1 | 298 | 15 | 313 | 1.1e-154 | 547.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053405.t1 | G7I5D1 | 99.1 | 322 | 3 | 0 | 1 | 322 | 1 | 322 | 1.8e-190 | 674.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050762 | MS.gene053405 | 0.806939 | 6.45E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene053405 | 0.808698 | 2.73E-50 | -1.69E-46 |
| MS.gene053405 | MS.gene054379 | 0.813225 | 2.85E-51 | -1.69E-46 |
| MS.gene053405 | MS.gene64029 | 0.812718 | 3.68E-51 | -1.69E-46 |
| MS.gene053405 | MS.gene65499 | 0.89953 | 1.81E-77 | -1.69E-46 |
| MS.gene053405 | MS.gene65950 | 0.988561 | 1.67E-174 | -1.69E-46 |
| MS.gene053405 | MS.gene69794 | 0.800364 | 1.49E-48 | -1.69E-46 |
| MS.gene053405 | MS.gene78205 | 0.806727 | 7.15E-50 | -1.69E-46 |
| MS.gene053405 | MS.gene80298 | 0.827761 | 1.31E-54 | -1.69E-46 |
| MS.gene053405 | MS.gene84401 | 0.816443 | 5.52E-52 | -1.69E-46 |
| MS.gene053405 | MS.gene97270 | 0.811326 | 7.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053405.t1 | MTR_1g043240 | 99.068 | 322 | 3 | 0 | 1 | 322 | 1 | 322 | 0.0 | 663 |
| MS.gene053405.t1 | MTR_1g074880 | 82.569 | 327 | 48 | 2 | 1 | 319 | 1 | 326 | 0.0 | 570 |
| MS.gene053405.t1 | MTR_1g079950 | 81.651 | 327 | 51 | 2 | 1 | 319 | 30 | 355 | 0.0 | 566 |
| MS.gene053405.t1 | MTR_1g079950 | 81.651 | 327 | 51 | 2 | 1 | 319 | 1 | 326 | 0.0 | 565 |
| MS.gene053405.t1 | MTR_1g074880 | 84.345 | 313 | 48 | 1 | 1 | 313 | 1 | 312 | 0.0 | 563 |
| MS.gene053405.t1 | MTR_1g079950 | 81.931 | 321 | 57 | 1 | 1 | 321 | 1 | 320 | 0.0 | 563 |
| MS.gene053405.t1 | MTR_1g079950 | 81.931 | 321 | 57 | 1 | 1 | 321 | 1 | 320 | 0.0 | 561 |
| MS.gene053405.t1 | MTR_7g116380 | 82.263 | 327 | 49 | 2 | 1 | 319 | 1 | 326 | 0.0 | 560 |
| MS.gene053405.t1 | MTR_7g116380 | 82.243 | 321 | 56 | 1 | 1 | 321 | 1 | 320 | 0.0 | 555 |
| MS.gene053405.t1 | MTR_5g081230 | 83.278 | 299 | 49 | 1 | 1 | 298 | 17 | 315 | 0.0 | 529 |
| MS.gene053405.t1 | MTR_8g098980 | 75.161 | 310 | 75 | 2 | 1 | 309 | 1 | 309 | 9.32e-180 | 499 |
| MS.gene053405.t1 | MTR_8g005010 | 75.517 | 290 | 71 | 0 | 6 | 295 | 4 | 293 | 4.02e-173 | 482 |
| MS.gene053405.t1 | MTR_1g074880 | 82.156 | 269 | 39 | 2 | 59 | 319 | 4 | 271 | 2.18e-168 | 469 |
| MS.gene053405.t1 | MTR_1g079950 | 82.443 | 262 | 45 | 1 | 60 | 321 | 34 | 294 | 8.62e-168 | 469 |
| MS.gene053405.t1 | MTR_7g112870 | 71.661 | 307 | 82 | 2 | 1 | 307 | 5 | 306 | 2.39e-167 | 468 |
| MS.gene053405.t1 | MTR_1g074880 | 84.314 | 255 | 39 | 1 | 59 | 313 | 4 | 257 | 3.56e-166 | 463 |
| MS.gene053405.t1 | MTR_5g093990 | 48.134 | 268 | 138 | 1 | 26 | 292 | 17 | 284 | 1.26e-93 | 280 |
| MS.gene053405.t1 | MTR_7g107310 | 47.232 | 271 | 141 | 2 | 26 | 295 | 27 | 296 | 7.43e-92 | 276 |
| MS.gene053405.t1 | MTR_8g062430 | 45.455 | 286 | 154 | 2 | 26 | 310 | 20 | 304 | 9.16e-92 | 276 |
| MS.gene053405.t1 | MTR_5g037200 | 45.455 | 286 | 154 | 2 | 26 | 310 | 20 | 304 | 2.03e-90 | 272 |
| MS.gene053405.t1 | MTR_8g085610 | 46.125 | 271 | 144 | 2 | 26 | 295 | 22 | 291 | 4.08e-89 | 269 |
| MS.gene053405.t1 | MTR_8g085610 | 44.815 | 270 | 147 | 2 | 30 | 298 | 2 | 270 | 2.30e-87 | 264 |
| MS.gene053405.t1 | MTR_1g039380 | 44.487 | 263 | 143 | 3 | 25 | 285 | 15 | 276 | 9.57e-87 | 263 |
| MS.gene053405.t1 | MTR_1g039380 | 44.980 | 249 | 134 | 3 | 39 | 285 | 5 | 252 | 2.85e-84 | 256 |
| MS.gene053405.t1 | MTR_2g087530 | 46.405 | 306 | 143 | 9 | 9 | 295 | 644 | 947 | 4.06e-80 | 262 |
| MS.gene053405.t1 | MTR_1g089370 | 47.143 | 280 | 134 | 5 | 30 | 295 | 552 | 831 | 6.46e-80 | 260 |
| MS.gene053405.t1 | MTR_2g087530 | 46.230 | 305 | 145 | 8 | 9 | 295 | 531 | 834 | 6.69e-78 | 255 |
| MS.gene053405.t1 | MTR_4g050540 | 46.230 | 305 | 145 | 8 | 9 | 295 | 649 | 952 | 1.45e-77 | 255 |
| MS.gene053405.t1 | MTR_3g452710 | 42.292 | 253 | 139 | 4 | 33 | 279 | 195 | 446 | 1.40e-67 | 219 |
| MS.gene053405.t1 | MTR_3g452710 | 42.292 | 253 | 139 | 4 | 33 | 279 | 262 | 513 | 5.94e-67 | 219 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 151 | 5 | 22 | 279 | 102 | 365 | 9.68e-64 | 207 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 151 | 5 | 22 | 279 | 172 | 435 | 7.50e-63 | 206 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 151 | 5 | 22 | 279 | 211 | 474 | 1.26e-62 | 207 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 151 | 5 | 22 | 279 | 201 | 464 | 1.34e-62 | 206 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 151 | 5 | 22 | 279 | 211 | 474 | 1.56e-62 | 207 |
| MS.gene053405.t1 | MTR_2g100130 | 38.014 | 292 | 161 | 7 | 22 | 295 | 172 | 461 | 1.77e-61 | 203 |
| MS.gene053405.t1 | MTR_2g100130 | 40.000 | 265 | 150 | 6 | 22 | 279 | 211 | 473 | 2.94e-61 | 203 |
| MS.gene053405.t1 | MTR_2g100130 | 40.392 | 255 | 144 | 5 | 22 | 269 | 211 | 464 | 6.99e-61 | 202 |
| MS.gene053405.t1 | MTR_2g100160 | 41.532 | 248 | 137 | 5 | 39 | 279 | 138 | 384 | 1.16e-56 | 189 |
| MS.gene053405.t1 | MTR_5g037200 | 50.299 | 167 | 82 | 1 | 26 | 191 | 20 | 186 | 2.34e-56 | 182 |
| MS.gene053405.t1 | MTR_8g072600 | 31.897 | 348 | 158 | 12 | 33 | 313 | 100 | 435 | 1.45e-40 | 147 |
| MS.gene053405.t1 | MTR_8g072600 | 33.468 | 248 | 119 | 7 | 33 | 235 | 100 | 346 | 1.31e-32 | 124 |
| MS.gene053405.t1 | MTR_2g099710 | 30.408 | 319 | 162 | 14 | 31 | 313 | 211 | 505 | 1.46e-23 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053405.t1 | AT2G39840 | 81.940 | 299 | 53 | 1 | 1 | 298 | 14 | 312 | 0.0 | 530 |
| MS.gene053405.t1 | AT2G29400 | 80.858 | 303 | 54 | 3 | 1 | 300 | 15 | 316 | 0.0 | 520 |
| MS.gene053405.t1 | AT5G59160 | 79.934 | 304 | 58 | 3 | 1 | 302 | 9 | 311 | 0.0 | 517 |
| MS.gene053405.t1 | AT5G59160 | 79.934 | 304 | 58 | 3 | 1 | 302 | 9 | 311 | 0.0 | 517 |
| MS.gene053405.t1 | AT5G59160 | 79.934 | 304 | 58 | 3 | 1 | 302 | 9 | 311 | 0.0 | 517 |
| MS.gene053405.t1 | AT5G43380 | 75.701 | 321 | 77 | 1 | 1 | 321 | 1 | 320 | 0.0 | 511 |
| MS.gene053405.t1 | AT5G43380 | 75.701 | 321 | 77 | 1 | 1 | 321 | 1 | 320 | 0.0 | 510 |
| MS.gene053405.t1 | AT5G43380 | 75.938 | 320 | 76 | 1 | 1 | 320 | 1 | 319 | 0.0 | 509 |
| MS.gene053405.t1 | AT3G46820 | 78.667 | 300 | 62 | 1 | 1 | 298 | 9 | 308 | 0.0 | 507 |
| MS.gene053405.t1 | AT1G64040 | 77.705 | 305 | 66 | 2 | 1 | 304 | 1 | 304 | 0.0 | 507 |
| MS.gene053405.t1 | AT4G11240 | 72.492 | 309 | 83 | 2 | 1 | 308 | 1 | 308 | 4.49e-174 | 485 |
| MS.gene053405.t1 | AT5G27840 | 71.290 | 310 | 80 | 2 | 1 | 303 | 9 | 316 | 4.97e-168 | 470 |
| MS.gene053405.t1 | AT3G05580 | 72.581 | 310 | 76 | 2 | 1 | 303 | 9 | 316 | 7.71e-168 | 469 |
| MS.gene053405.t1 | AT5G27840 | 71.429 | 308 | 82 | 2 | 1 | 304 | 9 | 314 | 1.01e-167 | 469 |
| MS.gene053405.t1 | AT5G27840 | 73.559 | 295 | 76 | 1 | 1 | 295 | 9 | 301 | 1.73e-167 | 469 |
| MS.gene053405.t1 | AT5G27840 | 73.559 | 295 | 76 | 1 | 1 | 295 | 9 | 301 | 1.73e-167 | 469 |
| MS.gene053405.t1 | AT3G05580 | 73.577 | 246 | 63 | 1 | 1 | 246 | 9 | 252 | 1.82e-134 | 382 |
| MS.gene053405.t1 | AT5G55260 | 48.507 | 268 | 137 | 1 | 26 | 292 | 17 | 284 | 4.13e-96 | 287 |
| MS.gene053405.t1 | AT4G26720 | 46.269 | 268 | 143 | 1 | 26 | 292 | 17 | 284 | 9.74e-94 | 281 |
| MS.gene053405.t1 | AT2G42500 | 47.080 | 274 | 143 | 2 | 26 | 298 | 27 | 299 | 2.40e-93 | 280 |
| MS.gene053405.t1 | AT3G58500 | 46.350 | 274 | 145 | 2 | 26 | 298 | 27 | 299 | 1.11e-91 | 276 |
| MS.gene053405.t1 | AT1G10430 | 44.755 | 286 | 156 | 2 | 26 | 310 | 20 | 304 | 1.05e-90 | 273 |
| MS.gene053405.t1 | AT1G10430 | 44.755 | 286 | 156 | 2 | 26 | 310 | 20 | 304 | 1.05e-90 | 273 |
| MS.gene053405.t1 | AT1G69960 | 45.105 | 286 | 155 | 2 | 26 | 310 | 21 | 305 | 2.30e-90 | 272 |
| MS.gene053405.t1 | AT1G59830 | 44.755 | 286 | 156 | 2 | 26 | 310 | 20 | 304 | 7.35e-90 | 271 |
| MS.gene053405.t1 | AT5G55260 | 42.071 | 309 | 137 | 2 | 26 | 292 | 17 | 325 | 1.52e-87 | 266 |
| MS.gene053405.t1 | AT3G19980 | 44.487 | 263 | 143 | 3 | 25 | 285 | 15 | 276 | 2.29e-86 | 262 |
| MS.gene053405.t1 | AT1G50370 | 44.106 | 263 | 144 | 3 | 25 | 285 | 15 | 276 | 3.54e-86 | 261 |
| MS.gene053405.t1 | AT4G03080 | 47.143 | 280 | 134 | 5 | 30 | 295 | 554 | 833 | 5.16e-80 | 260 |
| MS.gene053405.t1 | AT2G27210 | 45.902 | 305 | 146 | 8 | 9 | 295 | 650 | 953 | 4.88e-79 | 259 |
| MS.gene053405.t1 | AT2G27210 | 45.902 | 305 | 146 | 8 | 9 | 295 | 655 | 958 | 5.14e-79 | 259 |
| MS.gene053405.t1 | AT1G08420 | 46.230 | 305 | 145 | 8 | 9 | 295 | 661 | 964 | 9.27e-79 | 259 |
| MS.gene053405.t1 | AT1G08420 | 46.230 | 305 | 145 | 8 | 9 | 295 | 666 | 969 | 9.74e-79 | 259 |
| MS.gene053405.t1 | AT1G59830 | 48.908 | 229 | 115 | 2 | 26 | 253 | 20 | 247 | 3.05e-78 | 239 |
| MS.gene053405.t1 | AT2G42500 | 48.416 | 221 | 112 | 2 | 79 | 298 | 33 | 252 | 1.43e-74 | 230 |
| MS.gene053405.t1 | AT1G03445 | 43.662 | 284 | 143 | 7 | 26 | 294 | 476 | 757 | 2.07e-73 | 241 |
| MS.gene053405.t1 | AT1G03445 | 43.662 | 284 | 143 | 7 | 26 | 294 | 476 | 757 | 2.19e-73 | 241 |
| MS.gene053405.t1 | AT2G42810 | 42.688 | 253 | 138 | 4 | 33 | 279 | 197 | 448 | 4.46e-66 | 215 |
| MS.gene053405.t1 | AT2G42810 | 42.688 | 253 | 138 | 4 | 33 | 279 | 197 | 448 | 4.46e-66 | 215 |
| MS.gene053405.t1 | AT2G42810 | 42.688 | 253 | 138 | 4 | 33 | 279 | 197 | 448 | 4.46e-66 | 215 |
| MS.gene053405.t1 | AT2G42810 | 42.688 | 253 | 138 | 4 | 33 | 279 | 251 | 502 | 1.15e-65 | 216 |
| MS.gene053405.t1 | AT2G42810 | 42.688 | 253 | 138 | 4 | 33 | 279 | 250 | 501 | 1.34e-65 | 215 |
| MS.gene053405.t1 | AT2G42500 | 46.939 | 147 | 74 | 3 | 26 | 168 | 27 | 173 | 8.97e-43 | 146 |
| MS.gene053405.t1 | AT2G42500 | 46.104 | 154 | 77 | 4 | 26 | 175 | 27 | 178 | 2.93e-42 | 144 |
| MS.gene053405.t1 | AT2G42500 | 48.921 | 139 | 67 | 3 | 26 | 160 | 27 | 165 | 5.31e-42 | 144 |
| MS.gene053405.t1 | AT5G63870 | 30.205 | 341 | 170 | 13 | 33 | 306 | 52 | 391 | 3.72e-35 | 132 |
| MS.gene053405.t1 | AT5G63870 | 30.205 | 341 | 170 | 13 | 33 | 306 | 52 | 391 | 3.72e-35 | 132 |
| MS.gene053405.t1 | AT1G48120 | 30.872 | 298 | 146 | 6 | 31 | 268 | 628 | 925 | 1.73e-28 | 116 |
| MS.gene053405.t1 | AT5G63870 | 31.474 | 251 | 130 | 9 | 33 | 242 | 52 | 301 | 4.26e-28 | 110 |
| MS.gene053405.t1 | AT5G10900 | 28.276 | 290 | 149 | 11 | 55 | 302 | 225 | 497 | 2.28e-21 | 95.1 |
| MS.gene053405.t1 | AT5G10900 | 28.276 | 290 | 149 | 11 | 55 | 302 | 225 | 497 | 2.50e-21 | 95.1 |
| MS.gene053405.t1 | AT5G10900 | 28.276 | 290 | 149 | 11 | 55 | 302 | 225 | 497 | 2.81e-21 | 94.7 |
Find 66 sgRNAs with CRISPR-Local
Find 271 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCCAACTACTTGTTCTT+AGG | 0.194579 | 1.4:+27592418 | MS.gene053405:CDS |
| GAATCCGTAGACGCGATTTA+TGG | 0.207636 | 1.4:-27592554 | None:intergenic |
| TAGTTACCACATATCTTAAT+TGG | 0.282650 | 1.4:-27589776 | None:intergenic |
| TGTTTCAAACTATGCAATTC+AGG | 0.295203 | 1.4:-27592700 | None:intergenic |
| TCAAACTCTCCACAGTAATT+AGG | 0.300714 | 1.4:-27593662 | None:intergenic |
| CATGCTCAAATAGGCTTAAT+AGG | 0.316064 | 1.4:-27592381 | None:intergenic |
| CTTGAAGCAGCCTAATTTGT+TGG | 0.324778 | 1.4:+27589742 | MS.gene053405:CDS |
| TCACATAAAAGACCAGTTTC+AGG | 0.342012 | 1.4:-27592754 | None:intergenic |
| CGTGGTGTTTCATTTACTTT+TGG | 0.343305 | 1.4:+27592829 | MS.gene053405:CDS |
| GATCATAAAATGCCTAAGTT+TGG | 0.343821 | 1.4:+27593752 | MS.gene053405:CDS |
| ATTCATGGTTTCCTCGTAGA+AGG | 0.349139 | 1.4:-27592525 | None:intergenic |
| GGAATCCACCATGCTCAAAT+AGG | 0.355827 | 1.4:-27592390 | None:intergenic |
| CTTGATTTGTAGAGCTCATC+AGG | 0.356629 | 1.4:+27592894 | MS.gene053405:CDS |
| TTCTTGATGGTATCATTAAT+AGG | 0.371158 | 1.4:+27589630 | MS.gene053405:CDS |
| GCCTCAAGTTCCAACAAATT+AGG | 0.376724 | 1.4:-27589752 | None:intergenic |
| CCAAGTGAAGTTCCTGAAAC+TGG | 0.383450 | 1.4:+27592742 | MS.gene053405:CDS |
| TTTCTATAGGTTGTTGAAGA+TGG | 0.384025 | 1.4:+27593593 | MS.gene053405:intron |
| AGATTGTTTCAACTGTTTGC+CGG | 0.384374 | 1.4:+27592627 | MS.gene053405:CDS |
| AATAGGTTGCTTGATGTTAG+AGG | 0.400822 | 1.4:+27589647 | MS.gene053405:CDS |
| GGACCGTGGAAAACAAAGTT+TGG | 0.405620 | 1.4:+27592450 | MS.gene053405:CDS |
| GATTTATGGAAGCACATTCA+TGG | 0.406324 | 1.4:-27592540 | None:intergenic |
| TCAGGAACTTCACTTGGCCT+TGG | 0.409043 | 1.4:-27592736 | None:intergenic |
| TTACTTTCAGGAGATATTCA+TGG | 0.409622 | 1.4:+27592346 | MS.gene053405:intron |
| TAGAGGAAGACCTGGGAAAC+AGG | 0.412490 | 1.4:+27589664 | MS.gene053405:CDS |
| TAGTAAAGATGTTCATGGTT+GGG | 0.413408 | 1.4:+27592795 | MS.gene053405:CDS |
| CCAGTTTCAGGAACTTCACT+TGG | 0.414690 | 1.4:-27592742 | None:intergenic |
| GCAAGAGGAGATTCAATGTA+AGG | 0.431969 | 1.4:+27592587 | MS.gene053405:CDS |
| ATGGAACGAAGGGTTCTTGA+TGG | 0.433784 | 1.4:+27589617 | MS.gene053405:CDS |
| TAGGAGCACAACTTCATTCA+AGG | 0.442692 | 1.4:+27593778 | MS.gene053405:CDS |
| GGAGATTCAATGTAAGGGTA+TGG | 0.442978 | 1.4:+27592593 | MS.gene053405:CDS |
| GATCCTAGTAAAGATGTTCA+TGG | 0.464856 | 1.4:+27592790 | MS.gene053405:CDS |
| TTTCAACTGTTTGCCGGTCG+CGG | 0.466909 | 1.4:+27592633 | MS.gene053405:CDS |
| CAACCATGAACATCTTTACT+AGG | 0.473905 | 1.4:-27592793 | None:intergenic |
| TGTGGAGAGTTTGACAATGC+TGG | 0.482312 | 1.4:+27593671 | MS.gene053405:CDS |
| GCCTAATTTGTTGGAACTTG+AGG | 0.482437 | 1.4:+27589751 | MS.gene053405:CDS |
| GAAAGCTGAACCTGTTTCCC+AGG | 0.504004 | 1.4:-27589674 | None:intergenic |
| AGTAAAGATGTTCATGGTTG+GGG | 0.513074 | 1.4:+27592796 | MS.gene053405:CDS |
| GCTTCCATAAATCGCGTCTA+CGG | 0.514773 | 1.4:+27592550 | MS.gene053405:CDS |
| GTCACCTAAGAACAAGTAGT+TGG | 0.527172 | 1.4:-27592422 | None:intergenic |
| CTAGTAAAGATGTTCATGGT+TGG | 0.530691 | 1.4:+27592794 | MS.gene053405:CDS |
| TTTCTTGATTATGGAACGAA+GGG | 0.547346 | 1.4:+27589607 | None:intergenic |
| TCATCGATGATAGCCGCGAC+CGG | 0.549911 | 1.4:-27592646 | None:intergenic |
| CTTTCTTGATTATGGAACGA+AGG | 0.562095 | 1.4:+27589606 | None:intergenic |
| AACAGATAAGTAATTTGCCA+AGG | 0.564405 | 1.4:+27592719 | MS.gene053405:CDS |
| CTTGATGTTAGAGGAAGACC+TGG | 0.565129 | 1.4:+27589656 | MS.gene053405:CDS |
| GTGCTCCTAAAACCAAACTT+AGG | 0.573212 | 1.4:-27593764 | None:intergenic |
| GTGGAGAGTTTGACAATGCT+GGG | 0.589015 | 1.4:+27593672 | MS.gene053405:CDS |
| CAAGAGGAGATTCAATGTAA+GGG | 0.594417 | 1.4:+27592588 | MS.gene053405:CDS |
| CTTGTTCTTAGGTGACTATG+TGG | 0.595503 | 1.4:+27592429 | MS.gene053405:CDS |
| AAACAGGTTCAGCTTTCAGA+GGG | 0.596976 | 1.4:+27589680 | MS.gene053405:CDS |
| AGATTCAAGAGTTTCTTCAA+AGG | 0.599855 | 1.4:+27592860 | MS.gene053405:CDS |
| CTATTAAGCCTATTTGAGCA+TGG | 0.607320 | 1.4:+27592382 | MS.gene053405:CDS |
| GAGGCACCAATTAAGATATG+TGG | 0.612070 | 1.4:+27589770 | MS.gene053405:CDS |
| AGAAATCAAGCAGCTTTGTG+TGG | 0.618536 | 1.4:+27589703 | MS.gene053405:CDS |
| GAAACAGGTTCAGCTTTCAG+AGG | 0.619149 | 1.4:+27589679 | MS.gene053405:CDS |
| TTAAGCCTATTTGAGCATGG+TGG | 0.624084 | 1.4:+27592385 | MS.gene053405:CDS |
| GAAAAGATTATATGTATGCA+TGG | 0.633670 | 1.4:+27592667 | MS.gene053405:CDS |
| GATTCTACGACGAATGCAAG+AGG | 0.633685 | 1.4:+27592572 | MS.gene053405:CDS |
| ACAAGTAGTTGGAACGTGGA+GGG | 0.641155 | 1.4:-27592411 | None:intergenic |
| AACAAGTAGTTGGAACGTGG+AGG | 0.651695 | 1.4:-27592412 | None:intergenic |
| AAGAACAAGTAGTTGGAACG+TGG | 0.682620 | 1.4:-27592415 | None:intergenic |
| TTAGGTGACTATGTGGACCG+TGG | 0.683185 | 1.4:+27592436 | MS.gene053405:CDS |
| AAGATTATATGTATGCATGG+TGG | 0.692863 | 1.4:+27592670 | MS.gene053405:CDS |
| GGTTGGGGTGTTAATGAACG+TGG | 0.714430 | 1.4:+27592811 | MS.gene053405:CDS |
| TGGAGAGTTTGACAATGCTG+GGG | 0.744427 | 1.4:+27593673 | MS.gene053405:CDS |
| TTGATGTTAGAGGAAGACCT+GGG | 0.755374 | 1.4:+27589657 | MS.gene053405:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAGTAATAGTTT+AGG | + | chr1.4:27591513-27591532 | MS.gene053405:intron | 10.0% |
| !! | AAAACAAGAATTATTAAAAT+TGG | + | chr1.4:27594667-27594686 | MS.gene053405:intron | 10.0% |
| !! | AAACAAGAATTATTAAAATT+GGG | + | chr1.4:27594668-27594687 | MS.gene053405:intron | 10.0% |
| !! | ATGAGTATTTAAAAAAATTA+AGG | + | chr1.4:27593460-27593479 | MS.gene053405:intron | 10.0% |
| !!! | AAAATTACTTTTTTTATTCA+AGG | + | chr1.4:27593400-27593419 | MS.gene053405:intron | 10.0% |
| !!! | AAGAATTCTTTTTTTTTTTT+TGG | - | chr1.4:27592272-27592291 | None:intergenic | 10.0% |
| !!! | TATTTTATTTTTTTCTTGAA+GGG | + | chr1.4:27591984-27592003 | MS.gene053405:intron | 10.0% |
| !!! | TTATTTTATTTTTTTCTTGA+AGG | + | chr1.4:27591983-27592002 | MS.gene053405:intron | 10.0% |
| !! | AAAAAAGAAAAAAGAAAATG+AGG | - | chr1.4:27589810-27589829 | None:intergenic | 15.0% |
| !! | AAAGAATTAGAATTGATAAA+GGG | + | chr1.4:27591140-27591159 | MS.gene053405:intron | 15.0% |
| !! | AAATGAAACTAAATTTAACA+AGG | - | chr1.4:27591885-27591904 | None:intergenic | 15.0% |
| !! | AAATTATACATAACATAACA+TGG | + | chr1.4:27594145-27594164 | MS.gene053405:intron | 15.0% |
| !! | AATGAAACACAATAAAATAT+AGG | - | chr1.4:27594914-27594933 | None:intergenic | 15.0% |
| !! | TAAACAAAATGATTTATGTT+TGG | + | chr1.4:27590555-27590574 | MS.gene053405:intron | 15.0% |
| !! | TAAAGAATTAGAATTGATAA+AGG | + | chr1.4:27591139-27591158 | MS.gene053405:intron | 15.0% |
| !! | TTAAGTTTATGAATACAAAT+GGG | + | chr1.4:27594187-27594206 | MS.gene053405:intron | 15.0% |
| !!! | AAGTCGTTTTATATATTTTA+AGG | + | chr1.4:27591443-27591462 | MS.gene053405:intron | 15.0% |
| !!! | ACAAAATTTTTCTCAAATTT+AGG | - | chr1.4:27592996-27593015 | None:intergenic | 15.0% |
| !!! | CAAAATTTTTCTCAAATTTA+GGG | - | chr1.4:27592995-27593014 | None:intergenic | 15.0% |
| !!! | CTTTTTATTTTATTTTCAGA+AGG | + | chr1.4:27595013-27595032 | MS.gene053405:intron | 15.0% |
| !!! | TATTTTCTGTTTTAGATTTT+CGG | - | chr1.4:27594648-27594667 | None:intergenic | 15.0% |
| !!! | TGCTTATTATTATAACTTTT+TGG | - | chr1.4:27594718-27594737 | None:intergenic | 15.0% |
| !!! | TTAAAGTTTATGTTCATTTT+TGG | + | chr1.4:27591677-27591696 | MS.gene053405:intron | 15.0% |
| !! | AAAGAATGAGAATTGATAAA+GGG | + | chr1.4:27590646-27590665 | MS.gene053405:intron | 20.0% |
| !! | AAATACTAAGAAAACCTTAA+TGG | + | chr1.4:27594621-27594640 | MS.gene053405:intron | 20.0% |
| !! | AATAGTGTAAGTTTGATATT+TGG | - | chr1.4:27594749-27594768 | None:intergenic | 20.0% |
| !! | AATGATATGAAACTTAAGTA+AGG | - | chr1.4:27594301-27594320 | None:intergenic | 20.0% |
| !! | ACTAAAATATACATAGCTAA+AGG | + | chr1.4:27590614-27590633 | MS.gene053405:intron | 20.0% |
| !! | ACTCATATTTAATGATTAGT+GGG | - | chr1.4:27590179-27590198 | None:intergenic | 20.0% |
| !! | AGCAATTAGAATAATGTTAT+TGG | + | chr1.4:27593858-27593877 | MS.gene053405:intron | 20.0% |
| !! | ATAAATGAAATCTGCAAATT+TGG | + | chr1.4:27590380-27590399 | MS.gene053405:intron | 20.0% |
| !! | ATATACTTAACGTACTAATT+TGG | + | chr1.4:27594849-27594868 | MS.gene053405:intron | 20.0% |
| !! | ATGATATGAAACTTAAGTAA+GGG | - | chr1.4:27594300-27594319 | None:intergenic | 20.0% |
| !! | CAATTTATGAGCTAAATTAA+TGG | - | chr1.4:27590309-27590328 | None:intergenic | 20.0% |
| !! | CTTAAGTTTATGAATACAAA+TGG | + | chr1.4:27594186-27594205 | MS.gene053405:intron | 20.0% |
| !! | GGAACTAAAAACATATTTAA+CGG | - | chr1.4:27592941-27592960 | None:intergenic | 20.0% |
| !! | TAAAGAATGAGAATTGATAA+AGG | + | chr1.4:27590645-27590664 | MS.gene053405:intron | 20.0% |
| !!! | ATCAAATTTTGTTACATTCA+AGG | + | chr1.4:27589918-27589937 | MS.gene053405:intron | 20.0% |
| !!! | ATTATATTTTTGGAAAACAG+AGG | - | chr1.4:27591908-27591927 | None:intergenic | 20.0% |
| !!! | ATTTTTGGTCTAATTGAAAT+TGG | + | chr1.4:27591692-27591711 | MS.gene053405:intron | 20.0% |
| !!! | CTAAAAATGATAACACATTT+TGG | - | chr1.4:27594394-27594413 | None:intergenic | 20.0% |
| !!! | CTTTGATGTTTTTTTCATTT+TGG | + | chr1.4:27594242-27594261 | MS.gene053405:intron | 20.0% |
| !!! | TAAGAGAATTGATTTATGTT+TGG | + | chr1.4:27591005-27591024 | MS.gene053405:intron | 20.0% |
| !!! | TCAAATTTTGTTACATTCAA+GGG | + | chr1.4:27589919-27589938 | MS.gene053405:intron | 20.0% |
| !!! | TGAAATATCAATATTTCTGT+TGG | - | chr1.4:27593812-27593831 | None:intergenic | 20.0% |
| !!! | TGTTTTTTTCATTTTGGTTT+TGG | + | chr1.4:27594248-27594267 | MS.gene053405:intron | 20.0% |
| !!! | TTTTAGTCAATTTAAGTGTA+TGG | + | chr1.4:27589852-27589871 | MS.gene053405:intron | 20.0% |
| !!! | TTTTCAAAATGACAATGTTA+AGG | - | chr1.4:27594112-27594131 | None:intergenic | 20.0% |
| ! | AAAAACCACATAGAAAAACA+TGG | - | chr1.4:27589878-27589897 | None:intergenic | 25.0% |
| ! | AAAAGAGATTATGTCTAATG+AGG | - | chr1.4:27592134-27592153 | None:intergenic | 25.0% |
| ! | AATTCTTGTCTACTAAAACA+AGG | + | chr1.4:27592285-27592304 | MS.gene053405:intron | 25.0% |
| ! | ACTAAAATAGACATAGCTAA+AGG | + | chr1.4:27591108-27591127 | MS.gene053405:intron | 25.0% |
| ! | ACTTAGTCCTTGAAAAAAAT+CGG | + | chr1.4:27593291-27593310 | MS.gene053405:intron | 25.0% |
| ! | ATCCAAAATCTTAACTCTTA+AGG | - | chr1.4:27593166-27593185 | None:intergenic | 25.0% |
| ! | CATGAAAAAATTGGATCTTA+CGG | - | chr1.4:27593327-27593346 | None:intergenic | 25.0% |
| ! | GAAAAGATTATATGTATGCA+TGG | + | chr1.4:27592667-27592686 | MS.gene053405:CDS | 25.0% |
| ! | GAAATTTATGAAAGTGATGT+AGG | - | chr1.4:27594828-27594847 | None:intergenic | 25.0% |
| ! | GACTCATATTTAATGATTAG+TGG | - | chr1.4:27590180-27590199 | None:intergenic | 25.0% |
| ! | GGGAAAAAGAATAATGTTTA+AGG | + | chr1.4:27594207-27594226 | MS.gene053405:intron | 25.0% |
| ! | TAGAGTAGACATGAAAAAAT+TGG | - | chr1.4:27593336-27593355 | None:intergenic | 25.0% |
| ! | TAGTTACCACATATCTTAAT+TGG | - | chr1.4:27589779-27589798 | None:intergenic | 25.0% |
| ! | TCGAAGTTTCATGATTATAT+AGG | - | chr1.4:27592962-27592981 | None:intergenic | 25.0% |
| ! | TCTATAGACATCACATAATT+TGG | + | chr1.4:27591955-27591974 | MS.gene053405:intron | 25.0% |
| ! | TTCACTATGTTATTACTTTC+AGG | + | chr1.4:27592334-27592353 | MS.gene053405:intron | 25.0% |
| !! | CAAATTTTGTTACATTCAAG+GGG | + | chr1.4:27589920-27589939 | MS.gene053405:intron | 25.0% |
| !! | TGAGAGAATTGATTTATGTT+TGG | + | chr1.4:27590511-27590530 | MS.gene053405:intron | 25.0% |
| !! | TGGTATATTTTTCTACTTAG+TGG | + | chr1.4:27591627-27591646 | MS.gene053405:intron | 25.0% |
| !! | TGTTTGAAACTTCTGATTAT+TGG | - | chr1.4:27590213-27590232 | None:intergenic | 25.0% |
| !! | TTAAGAGTTAAGATTTTGGA+TGG | + | chr1.4:27593165-27593184 | MS.gene053405:intron | 25.0% |
| !! | TTCTTGATGGTATCATTAAT+AGG | + | chr1.4:27589630-27589649 | MS.gene053405:CDS | 25.0% |
| !! | TTTTATTCAAGGACCAAATT+AGG | + | chr1.4:27593411-27593430 | MS.gene053405:intron | 25.0% |
| !! | TTTTCAGATATTAAAACCTG+TGG | + | chr1.4:27593730-27593749 | MS.gene053405:CDS | 25.0% |
| !!! | AGAATTTTTTCAAATTCTGG+TGG | - | chr1.4:27594549-27594568 | None:intergenic | 25.0% |
| !!! | CAGAGAATTTTTTCAAATTC+TGG | - | chr1.4:27594552-27594571 | None:intergenic | 25.0% |
| !!! | TATTTTTTTCTTGAAGGGAA+AGG | + | chr1.4:27591989-27592008 | MS.gene053405:intron | 25.0% |
| !!! | TCTTGTGTTTTGTTTTCTAT+AGG | + | chr1.4:27593580-27593599 | MS.gene053405:intron | 25.0% |
| !!! | TTCAGGGTATTTTATTTTGT+AGG | - | chr1.4:27592497-27592516 | None:intergenic | 25.0% |
| !!! | TTTAACTTTTTTCAGAAGCA+CGG | + | chr1.4:27591599-27591618 | MS.gene053405:intron | 25.0% |
| !!! | TTTTGTTTTGACTTGAGTAT+TGG | + | chr1.4:27592150-27592169 | MS.gene053405:intron | 25.0% |
| !!! | TTTTTTCATGTCTACTCTAT+AGG | + | chr1.4:27593337-27593356 | MS.gene053405:intron | 25.0% |
| AAAATGACAATGTTAAGGTC+AGG | - | chr1.4:27594107-27594126 | None:intergenic | 30.0% | |
| AAAATTAGCGACACCTAATT+TGG | - | chr1.4:27593427-27593446 | None:intergenic | 30.0% | |
| AACCTTATTAAAGCAACCTA+TGG | + | chr1.4:27591793-27591812 | MS.gene053405:intron | 30.0% | |
| AAGATTATATGTATGCATGG+TGG | + | chr1.4:27592670-27592689 | MS.gene053405:CDS | 30.0% | |
| AATCAAAATTGAACCCAACA+TGG | - | chr1.4:27593072-27593091 | None:intergenic | 30.0% | |
| AGATTCAAGAGTTTCTTCAA+AGG | + | chr1.4:27592860-27592879 | MS.gene053405:CDS | 30.0% | |
| ATATTCAAAGCTGAACCAAA+CGG | - | chr1.4:27590437-27590456 | None:intergenic | 30.0% | |
| ATTCAGTGTGTTATAACTAG+TGG | + | chr1.4:27591832-27591851 | MS.gene053405:intron | 30.0% | |
| ATTCTTGGATTATCCTTAGT+CGG | + | chr1.4:27594439-27594458 | MS.gene053405:intron | 30.0% | |
| CATAGTACATAGATTTGAGT+AGG | + | chr1.4:27594342-27594361 | MS.gene053405:intron | 30.0% | |
| CTTAGTCCTTGAAAAAAATC+GGG | + | chr1.4:27593292-27593311 | MS.gene053405:intron | 30.0% | |
| GATCATAAAATGCCTAAGTT+TGG | + | chr1.4:27593752-27593771 | MS.gene053405:CDS | 30.0% | |
| GTTAAACCTAGATTGAAAAG+AGG | + | chr1.4:27594480-27594499 | MS.gene053405:intron | 30.0% | |
| TAGTAAAGATGTTCATGGTT+GGG | + | chr1.4:27592795-27592814 | MS.gene053405:CDS | 30.0% | |
| TATCGTTGAGAAAAAAAACC+AGG | - | chr1.4:27593381-27593400 | None:intergenic | 30.0% | |
| TGCTTAACATTTGCAAATTC+TGG | - | chr1.4:27593842-27593861 | None:intergenic | 30.0% | |
| TGTTTCAAACTATGCAATTC+AGG | - | chr1.4:27592703-27592722 | None:intergenic | 30.0% | |
| TTAACAAGGTAACTGAATCA+TGG | - | chr1.4:27591871-27591890 | None:intergenic | 30.0% | |
| TTACTTTCAGGAGATATTCA+TGG | + | chr1.4:27592346-27592365 | MS.gene053405:intron | 30.0% | |
| TTTATGAAAGTGATGTAGGA+AGG | - | chr1.4:27594824-27594843 | None:intergenic | 30.0% | |
| TTTCTATAGGTTGTTGAAGA+TGG | + | chr1.4:27593593-27593612 | MS.gene053405:intron | 30.0% | |
| ! | AACAGATAAGTAATTTGCCA+AGG | + | chr1.4:27592719-27592738 | MS.gene053405:CDS | 30.0% |
| ! | AACTTTCCTCTTTTCAATCT+AGG | - | chr1.4:27594489-27594508 | None:intergenic | 30.0% |
| ! | AGTCAATAGTTTGTCATTCT+TGG | + | chr1.4:27594424-27594443 | MS.gene053405:intron | 30.0% |
| ! | ATCTTAACTCTTAAGGCATT+AGG | - | chr1.4:27593159-27593178 | None:intergenic | 30.0% |
| ! | CTATTTTACAATTCTCTTCG+AGG | + | chr1.4:27592046-27592065 | MS.gene053405:intron | 30.0% |
| ! | CTCTTAGTGCTAATTTTAGA+TGG | + | chr1.4:27592206-27592225 | MS.gene053405:intron | 30.0% |
| ! | GCTAATGTTACTGCTATTTA+GGG | - | chr1.4:27591409-27591428 | None:intergenic | 30.0% |
| ! | GTAGAAGGAAAAAGTTTTCA+GGG | - | chr1.4:27592513-27592532 | None:intergenic | 30.0% |
| ! | GTCTACTAAAACAAGGTATT+AGG | + | chr1.4:27592292-27592311 | MS.gene053405:intron | 30.0% |
| ! | GTCTTTTATGTGATCTTTTG+TGG | + | chr1.4:27592764-27592783 | MS.gene053405:CDS | 30.0% |
| ! | TGCCTTAAGAGTTAAGATTT+TGG | + | chr1.4:27593161-27593180 | MS.gene053405:intron | 30.0% |
| ! | TTCCATAGGTTGCTTTAATA+AGG | - | chr1.4:27591798-27591817 | None:intergenic | 30.0% |
| ! | TTTAGATTTTCGGTCCATTA+AGG | - | chr1.4:27594638-27594657 | None:intergenic | 30.0% |
| !! | ATGTTGGGTTCAATTTTGAT+TGG | + | chr1.4:27593071-27593090 | MS.gene053405:intron | 30.0% |
| !! | CACTGTAGAGAATTGATTTT+GGG | + | chr1.4:27590855-27590874 | MS.gene053405:intron | 30.0% |
| !!! | AAATGCCTAAGTTTGGTTTT+AGG | + | chr1.4:27593759-27593778 | MS.gene053405:CDS | 30.0% |
| !!! | TATTTTCAGAAGGCATTTCT+TGG | + | chr1.4:27595023-27595042 | MS.gene053405:intron | 30.0% |
| !!! | TTTCCAAACTTTGTTTTCCA+CGG | - | chr1.4:27592456-27592475 | None:intergenic | 30.0% |
| !!! | TTTTAGAACCCTATTTTCAC+AGG | - | chr1.4:27594525-27594544 | None:intergenic | 30.0% |
| !!! | TTTTTGGCTAGAATTGCTTT+TGG | + | chr1.4:27590832-27590851 | MS.gene053405:intron | 30.0% |
| AAACCTATACACGTTGTTCA+AGG | + | chr1.4:27590708-27590727 | MS.gene053405:intron | 35.0% | |
| AACGACTTAGAGAGTTGAAA+CGG | - | chr1.4:27590073-27590092 | None:intergenic | 35.0% | |
| AACGACTTAGAGAGTTGAAA+TGG | - | chr1.4:27590073-27590092 | None:intergenic | 35.0% | |
| AATAGGTTGCTTGATGTTAG+AGG | + | chr1.4:27589647-27589666 | MS.gene053405:CDS | 35.0% | |
| ACCAATCTATATCCACTTTG+TGG | + | chr1.4:27590236-27590255 | MS.gene053405:intron | 35.0% | |
| AGATTGTTTCAACTGTTTGC+CGG | + | chr1.4:27592627-27592646 | MS.gene053405:CDS | 35.0% | |
| AGCTAGGATTTATAGCTTCA+TGG | + | chr1.4:27590755-27590774 | MS.gene053405:intron | 35.0% | |
| AGTAAAGATGTTCATGGTTG+GGG | + | chr1.4:27592796-27592815 | MS.gene053405:CDS | 35.0% | |
| ATCTATATCCACTTTGTGGA+AGG | + | chr1.4:27590240-27590259 | MS.gene053405:intron | 35.0% | |
| CAACCATGAACATCTTTACT+AGG | - | chr1.4:27592796-27592815 | None:intergenic | 35.0% | |
| CAAGAGGAGATTCAATGTAA+GGG | + | chr1.4:27592588-27592607 | MS.gene053405:CDS | 35.0% | |
| CATGCTCAAATAGGCTTAAT+AGG | - | chr1.4:27592384-27592403 | None:intergenic | 35.0% | |
| CCTTGCAAAACTCATCATTT+AGG | - | chr1.4:27594065-27594084 | None:intergenic | 35.0% | |
| CTAGTAAAGATGTTCATGGT+TGG | + | chr1.4:27592794-27592813 | MS.gene053405:CDS | 35.0% | |
| CTATTAAGCCTATTTGAGCA+TGG | + | chr1.4:27592382-27592401 | MS.gene053405:CDS | 35.0% | |
| GAACATGAATGTTGTTCCAT+AGG | - | chr1.4:27591812-27591831 | None:intergenic | 35.0% | |
| GATCCTAGTAAAGATGTTCA+TGG | + | chr1.4:27592790-27592809 | MS.gene053405:CDS | 35.0% | |
| GATTATGTCTAATGAGGTGT+CGG | - | chr1.4:27592128-27592147 | None:intergenic | 35.0% | |
| GTTACATTCAAGGGGATATA+AGG | + | chr1.4:27589928-27589947 | MS.gene053405:intron | 35.0% | |
| TATTCAAAGCTGAACCAAAC+GGG | - | chr1.4:27590436-27590455 | None:intergenic | 35.0% | |
| TCAAACTCTCCACAGTAATT+AGG | - | chr1.4:27593665-27593684 | None:intergenic | 35.0% | |
| TCACATAAAAGACCAGTTTC+AGG | - | chr1.4:27592757-27592776 | None:intergenic | 35.0% | |
| TCCACAAAGTGGATATAGAT+TGG | - | chr1.4:27590240-27590259 | None:intergenic | 35.0% | |
| TGAATGAAAAGATGTCTCTC+TGG | + | chr1.4:27594581-27594600 | MS.gene053405:intron | 35.0% | |
| ! | AAATGATGAGTTTTGCAAGG+AGG | + | chr1.4:27594065-27594084 | MS.gene053405:intron | 35.0% |
| ! | ATTTTTCTCAGCGACCAAAT+CGG | - | chr1.4:27593263-27593282 | None:intergenic | 35.0% |
| ! | CCTAAATGATGAGTTTTGCA+AGG | + | chr1.4:27594062-27594081 | MS.gene053405:intron | 35.0% |
| ! | CGTAGAAGGAAAAAGTTTTC+AGG | - | chr1.4:27592514-27592533 | None:intergenic | 35.0% |
| ! | CGTGGTGTTTCATTTACTTT+TGG | + | chr1.4:27592829-27592848 | MS.gene053405:CDS | 35.0% |
| ! | GATTTATGGAAGCACATTCA+TGG | - | chr1.4:27592543-27592562 | None:intergenic | 35.0% |
| ! | GGCTAATGTTACTGCTATTT+AGG | - | chr1.4:27591410-27591429 | None:intergenic | 35.0% |
| ! | TATGGCCATGTTTTTCTATG+TGG | + | chr1.4:27589870-27589889 | MS.gene053405:intron | 35.0% |
| ! | TCAAAACTAAGACTCTGAGA+AGG | - | chr1.4:27592184-27592203 | None:intergenic | 35.0% |
| ! | TTAGGCATTTTATGATCCAC+AGG | - | chr1.4:27593749-27593768 | None:intergenic | 35.0% |
| ! | TTCTAGAAAAGCTAGAAGCT+AGG | + | chr1.4:27590739-27590758 | MS.gene053405:intron | 35.0% |
| ! | TTTTCTGCTCCTAATTACTG+TGG | + | chr1.4:27593653-27593672 | MS.gene053405:CDS | 35.0% |
| !! | ATGTGCAAATTTGCTAAGAG+TGG | - | chr1.4:27590015-27590034 | None:intergenic | 35.0% |
| !! | GAAAACTTTTTCCTTCTACG+AGG | + | chr1.4:27592514-27592533 | MS.gene053405:CDS | 35.0% |
| !! | GCACTGTAGAGAATTGATTT+TGG | + | chr1.4:27590854-27590873 | MS.gene053405:intron | 35.0% |
| !! | TGAACCAAACACGTTGTTTT+TGG | + | chr1.4:27590816-27590835 | MS.gene053405:intron | 35.0% |
| !! | TGTGCAAATTTGCTAAGAGT+GGG | - | chr1.4:27590014-27590033 | None:intergenic | 35.0% |
| !!! | TAGTTGCCCGATTTTTTTCA+AGG | - | chr1.4:27593301-27593320 | None:intergenic | 35.0% |
| !!! | TTAGGTGTCGCTAATTTTCT+TGG | + | chr1.4:27593429-27593448 | MS.gene053405:intron | 35.0% |
| AAACAGGTTCAGCTTTCAGA+GGG | + | chr1.4:27589680-27589699 | MS.gene053405:CDS | 40.0% | |
| AAATTCATCAAGTGCCCGTT+TGG | + | chr1.4:27590419-27590438 | MS.gene053405:intron | 40.0% | |
| AAGAACAAGTAGTTGGAACG+TGG | - | chr1.4:27592418-27592437 | None:intergenic | 40.0% | |
| ACCAAACGGATGTTAAAGAC+AGG | - | chr1.4:27590917-27590936 | None:intergenic | 40.0% | |
| AGCTAGGATTTGTAGCTTCA+TGG | + | chr1.4:27591249-27591268 | MS.gene053405:intron | 40.0% | |
| ATTCATGGTTTCCTCGTAGA+AGG | - | chr1.4:27592528-27592547 | None:intergenic | 40.0% | |
| ATTGACCAAGAGAATGTTGG+AGG | - | chr1.4:27593231-27593250 | None:intergenic | 40.0% | |
| CAAACGGATGTTAAAGACAG+GGG | - | chr1.4:27590915-27590934 | None:intergenic | 40.0% | |
| CATTCAAGGGGATATAAGGT+TGG | + | chr1.4:27589932-27589951 | MS.gene053405:intron | 40.0% | |
| CCAAACGGATGTTAAAGACA+GGG | - | chr1.4:27590916-27590935 | None:intergenic | 40.0% | |
| CGTTCCAACTACTTGTTCTT+AGG | + | chr1.4:27592418-27592437 | MS.gene053405:CDS | 40.0% | |
| CTAGCCAAAAACAACGTGTT+TGG | - | chr1.4:27590823-27590842 | None:intergenic | 40.0% | |
| CTTGTTCTTAGGTGACTATG+TGG | + | chr1.4:27592429-27592448 | MS.gene053405:CDS | 40.0% | |
| GCAAGAGGAGATTCAATGTA+AGG | + | chr1.4:27592587-27592606 | MS.gene053405:CDS | 40.0% | |
| GCAATTGCTCGTACTATGTT+GGG | - | chr1.4:27590130-27590149 | None:intergenic | 40.0% | |
| GCCTCAAGTTCCAACAAATT+AGG | - | chr1.4:27589755-27589774 | None:intergenic | 40.0% | |
| GGAGATTCAATGTAAGGGTA+TGG | + | chr1.4:27592593-27592612 | MS.gene053405:CDS | 40.0% | |
| GGTACAAGAAACAAAGAGAC+AGG | + | chr1.4:27594363-27594382 | MS.gene053405:intron | 40.0% | |
| GTCACCTAAGAACAAGTAGT+TGG | - | chr1.4:27592425-27592444 | None:intergenic | 40.0% | |
| GTGCTCCTAAAACCAAACTT+AGG | - | chr1.4:27593767-27593786 | None:intergenic | 40.0% | |
| GTTCAGATCTCTTGTGGAAT+CGG | + | chr1.4:27593187-27593206 | MS.gene053405:intron | 40.0% | |
| GTTTGACCTTGGAACATCAA+GGG | - | chr1.4:27592094-27592113 | None:intergenic | 40.0% | |
| TCTCCTTGAACAACGTGTAT+AGG | - | chr1.4:27590714-27590733 | None:intergenic | 40.0% | |
| TGAAAGTGATGTAGGAAGGT+TGG | - | chr1.4:27594820-27594839 | None:intergenic | 40.0% | |
| TGAAGTGACGCATAAGAAAG+AGG | - | chr1.4:27594781-27594800 | None:intergenic | 40.0% | |
| TGGATTGACCAAGAGAATGT+TGG | - | chr1.4:27593234-27593253 | None:intergenic | 40.0% | |
| TTAAGCCTATTTGAGCATGG+TGG | + | chr1.4:27592385-27592404 | MS.gene053405:CDS | 40.0% | |
| TTACGAGGGTAGTGATAAGA+TGG | + | chr1.4:27589974-27589993 | MS.gene053405:intron | 40.0% | |
| TTGATGTTAGAGGAAGACCT+GGG | + | chr1.4:27589657-27589676 | MS.gene053405:CDS | 40.0% | |
| TTGTGTGTGCAAGTTTACGA+GGG | + | chr1.4:27589960-27589979 | MS.gene053405:intron | 40.0% | |
| ! | AAGGGGATATAAGGTTGGTT+TGG | + | chr1.4:27589937-27589956 | MS.gene053405:intron | 40.0% |
| ! | AGAAATCAAGCAGCTTTGTG+TGG | + | chr1.4:27589703-27589722 | MS.gene053405:CDS | 40.0% |
| ! | CTAGAATTGCTTCTGAGAAG+TGG | + | chr1.4:27590878-27590897 | MS.gene053405:intron | 40.0% |
| ! | CTTGAAGCAGCCTAATTTGT+TGG | + | chr1.4:27589742-27589761 | MS.gene053405:CDS | 40.0% |
| ! | GAGGCACCAATTAAGATATG+TGG | + | chr1.4:27589770-27589789 | MS.gene053405:CDS | 40.0% |
| ! | GCTTCATGGAGAATTGCTTT+TGG | + | chr1.4:27590769-27590788 | MS.gene053405:intron | 40.0% |
| ! | TAGGAGCACAACTTCATTCA+AGG | + | chr1.4:27593778-27593797 | MS.gene053405:CDS | 40.0% |
| ! | TATTTAGGGCCTGTATGGTT+TGG | - | chr1.4:27591395-27591414 | None:intergenic | 40.0% |
| ! | TTATCCTTAGTCGGAAGCAT+AGG | + | chr1.4:27594448-27594467 | MS.gene053405:intron | 40.0% |
| ! | TTTTTCTCAGCGACCAAATC+GGG | - | chr1.4:27593262-27593281 | None:intergenic | 40.0% |
| !! | CTTGATTTGTAGAGCTCATC+AGG | + | chr1.4:27592894-27592913 | MS.gene053405:CDS | 40.0% |
| !! | GCCTAATTTGTTGGAACTTG+AGG | + | chr1.4:27589751-27589770 | MS.gene053405:CDS | 40.0% |
| !! | GTGCAAATTTGCTAAGAGTG+GGG | - | chr1.4:27590013-27590032 | None:intergenic | 40.0% |
| !! | TCATGGAGAATTGCTTTTGG+AGG | + | chr1.4:27590772-27590791 | MS.gene053405:intron | 40.0% |
| !! | TTTTCAGAAGCACGGTTTGT+TGG | + | chr1.4:27591607-27591626 | MS.gene053405:intron | 40.0% |
| AACAAGTAGTTGGAACGTGG+AGG | - | chr1.4:27592415-27592434 | None:intergenic | 45.0% | |
| ACAAGTAGTTGGAACGTGGA+GGG | - | chr1.4:27592414-27592433 | None:intergenic | 45.0% | |
| ACCCAACATGGTCTCGAAAT+TGG | - | chr1.4:27593060-27593079 | None:intergenic | 45.0% | |
| ACCCAATTTCGAGACCATGT+TGG | + | chr1.4:27593055-27593074 | MS.gene053405:intron | 45.0% | |
| AGCGCGAAACCTGTGAAAAT+AGG | + | chr1.4:27594513-27594532 | MS.gene053405:intron | 45.0% | |
| AGGGTAGTGATAAGATGGTC+GGG | + | chr1.4:27589979-27589998 | MS.gene053405:intron | 45.0% | |
| ATGGAACGAAGGGTTCTTGA+TGG | + | chr1.4:27589617-27589636 | MS.gene053405:CDS | 45.0% | |
| CCAAGTGAAGTTCCTGAAAC+TGG | + | chr1.4:27592742-27592761 | MS.gene053405:CDS | 45.0% | |
| CCAGGAAAACTTCATCCTTG+AGG | - | chr1.4:27593363-27593382 | None:intergenic | 45.0% | |
| CCAGTTTCAGGAACTTCACT+TGG | - | chr1.4:27592745-27592764 | None:intergenic | 45.0% | |
| CCCAACATGGTCTCGAAATT+GGG | - | chr1.4:27593059-27593078 | None:intergenic | 45.0% | |
| CCCAATTTCGAGACCATGTT+GGG | + | chr1.4:27593056-27593075 | MS.gene053405:intron | 45.0% | |
| CCCTGTCTTTAACATCCGTT+TGG | + | chr1.4:27590913-27590932 | MS.gene053405:intron | 45.0% | |
| CTAACCTATGCTTCCGACTA+AGG | - | chr1.4:27594455-27594474 | None:intergenic | 45.0% | |
| CTCTTGTGGAATCGGATCAT+TGG | + | chr1.4:27593195-27593214 | MS.gene053405:intron | 45.0% | |
| CTTGATGTTAGAGGAAGACC+TGG | + | chr1.4:27589656-27589675 | MS.gene053405:CDS | 45.0% | |
| CTTTGTCCCTTGATGTTCCA+AGG | + | chr1.4:27592085-27592104 | MS.gene053405:intron | 45.0% | |
| GAAACAGGTTCAGCTTTCAG+AGG | + | chr1.4:27589679-27589698 | MS.gene053405:CDS | 45.0% | |
| GAATCCGTAGACGCGATTTA+TGG | - | chr1.4:27592557-27592576 | None:intergenic | 45.0% | |
| GAGGGTAGTGATAAGATGGT+CGG | + | chr1.4:27589978-27589997 | MS.gene053405:intron | 45.0% | |
| GATTCTACGACGAATGCAAG+AGG | + | chr1.4:27592572-27592591 | MS.gene053405:CDS | 45.0% | |
| GCAAGGAGGATTACTCTCAT+TGG | + | chr1.4:27594079-27594098 | MS.gene053405:intron | 45.0% | |
| GCGCGAAACCTGTGAAAATA+GGG | + | chr1.4:27594514-27594533 | MS.gene053405:intron | 45.0% | |
| GCTTCCATAAATCGCGTCTA+CGG | + | chr1.4:27592550-27592569 | MS.gene053405:CDS | 45.0% | |
| GGAATCCACCATGCTCAAAT+AGG | - | chr1.4:27592393-27592412 | None:intergenic | 45.0% | |
| GGACCGTGGAAAACAAAGTT+TGG | + | chr1.4:27592450-27592469 | MS.gene053405:CDS | 45.0% | |
| GGCAATTGCTCGTACTATGT+TGG | - | chr1.4:27590131-27590150 | None:intergenic | 45.0% | |
| GGTTTGACCTTGGAACATCA+AGG | - | chr1.4:27592095-27592114 | None:intergenic | 45.0% | |
| GTGGAGAGTTTGACAATGCT+GGG | + | chr1.4:27593672-27593691 | MS.gene053405:CDS | 45.0% | |
| GTTGTGTGTGCAAGTTTACG+AGG | + | chr1.4:27589959-27589978 | MS.gene053405:intron | 45.0% | |
| TATCCACTTTGTGGAAGGCT+TGG | + | chr1.4:27590245-27590264 | MS.gene053405:intron | 45.0% | |
| TCAATCCAACAGACCCGATT+TGG | + | chr1.4:27593246-27593265 | MS.gene053405:intron | 45.0% | |
| TCTCAGAAGCCAAACCATAC+AGG | + | chr1.4:27591383-27591402 | MS.gene053405:intron | 45.0% | |
| TCTGAGAAGTGGAACCAAAC+AGG | + | chr1.4:27590889-27590908 | MS.gene053405:intron | 45.0% | |
| TGGAGAGTTTGACAATGCTG+GGG | + | chr1.4:27593673-27593692 | MS.gene053405:CDS | 45.0% | |
| TGGATGGTTCAGATCTCTTG+TGG | + | chr1.4:27593181-27593200 | MS.gene053405:intron | 45.0% | |
| TGGTTACTCTCTGTTGCTAC+TGG | + | chr1.4:27591712-27591731 | MS.gene053405:intron | 45.0% | |
| TGTCTACTCTATAGGCCTCA+AGG | + | chr1.4:27593345-27593364 | MS.gene053405:intron | 45.0% | |
| TGTGGAGAGTTTGACAATGC+TGG | + | chr1.4:27593671-27593690 | MS.gene053405:CDS | 45.0% | |
| TGTTGCCTCCAACATTCTCT+TGG | + | chr1.4:27593223-27593242 | MS.gene053405:intron | 45.0% | |
| TTACCAAGCCTTCCACAAAG+TGG | - | chr1.4:27590251-27590270 | None:intergenic | 45.0% | |
| ! | ACTGCTATTTAGGGCCTGTA+TGG | - | chr1.4:27591400-27591419 | None:intergenic | 45.0% |
| ! | CCTCAAGGATGAAGTTTTCC+TGG | + | chr1.4:27593360-27593379 | MS.gene053405:intron | 45.0% |
| ! | TCTCTGTTGCTACTGGTTAG+TGG | + | chr1.4:27591719-27591738 | MS.gene053405:intron | 45.0% |
| !!! | TAAATCATTAATTATATTTT+TGG | - | chr1.4:27591918-27591937 | None:intergenic | 5.0% |
| !!! | TTTTTTTTTTTTTTTTTTTG+AGG | + | chr1.4:27591770-27591789 | MS.gene053405:intron | 5.0% |
| GAAAGCTGAACCTGTTTCCC+AGG | - | chr1.4:27589677-27589696 | None:intergenic | 50.0% | |
| GGGTAGTGATAAGATGGTCG+GGG | + | chr1.4:27589980-27589999 | MS.gene053405:intron | 50.0% | |
| GTTAAAGACAGGGGCCTGTT+TGG | - | chr1.4:27590906-27590925 | None:intergenic | 50.0% | |
| TAGAGGAAGACCTGGGAAAC+AGG | + | chr1.4:27589664-27589683 | MS.gene053405:CDS | 50.0% | |
| TCAGGAACTTCACTTGGCCT+TGG | - | chr1.4:27592739-27592758 | None:intergenic | 50.0% | |
| TTAGGTGACTATGTGGACCG+TGG | + | chr1.4:27592436-27592455 | MS.gene053405:CDS | 50.0% | |
| TTTCAACTGTTTGCCGGTCG+CGG | + | chr1.4:27592633-27592652 | MS.gene053405:CDS | 50.0% | |
| ! | GGTTGGGGTGTTAATGAACG+TGG | + | chr1.4:27592811-27592830 | MS.gene053405:CDS | 50.0% |
| !! | CTCGGTGTTAGGTTTGACCT+TGG | - | chr1.4:27592105-27592124 | None:intergenic | 50.0% |
| !! | TGTCTAATGAGGTGTCGGCT+CGG | - | chr1.4:27592123-27592142 | None:intergenic | 50.0% |
| AGCGACCAAATCGGGTCTGT+TGG | - | chr1.4:27593254-27593273 | None:intergenic | 55.0% | |
| GTACGAGCAATTGCCCAGCT+AGG | + | chr1.4:27590136-27590155 | MS.gene053405:intron | 55.0% | |
| TCATCGATGATAGCCGCGAC+CGG | - | chr1.4:27592649-27592668 | None:intergenic | 55.0% | |
| ACGCTCGAGCATAGCCTAGC+TGG | - | chr1.4:27590153-27590172 | None:intergenic | 60.0% | |
| CGCTCGAGCATAGCCTAGCT+GGG | - | chr1.4:27590152-27590171 | None:intergenic | 60.0% | |
| !! | TGAGGTGTCGGCTCGGTGTT+AGG | - | chr1.4:27592116-27592135 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 27589617 | 27595067 | 27589617 | ID=MS.gene053405 |
| chr1.4 | mRNA | 27589617 | 27595067 | 27589617 | ID=MS.gene053405.t1;Parent=MS.gene053405 |
| chr1.4 | exon | 27589617 | 27589791 | 27589617 | ID=MS.gene053405.t1.exon1;Parent=MS.gene053405.t1 |
| chr1.4 | CDS | 27589617 | 27589791 | 27589617 | ID=cds.MS.gene053405.t1;Parent=MS.gene053405.t1 |
| chr1.4 | exon | 27592356 | 27592915 | 27592356 | ID=MS.gene053405.t1.exon2;Parent=MS.gene053405.t1 |
| chr1.4 | CDS | 27592356 | 27592915 | 27592356 | ID=cds.MS.gene053405.t1;Parent=MS.gene053405.t1 |
| chr1.4 | exon | 27593602 | 27593799 | 27593602 | ID=MS.gene053405.t1.exon3;Parent=MS.gene053405.t1 |
| chr1.4 | CDS | 27593602 | 27593799 | 27593602 | ID=cds.MS.gene053405.t1;Parent=MS.gene053405.t1 |
| chr1.4 | exon | 27595032 | 27595067 | 27595032 | ID=MS.gene053405.t1.exon4;Parent=MS.gene053405.t1 |
| chr1.4 | CDS | 27595032 | 27595067 | 27595032 | ID=cds.MS.gene053405.t1;Parent=MS.gene053405.t1 |
| Gene Sequence |
| Protein sequence |
