AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78205


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78205.t1 MTR_0026s0010 73.944 142 34 2 22 161 25 165 8.97e-65 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78205.t1 AT1G10155 41.558 154 71 6 24 159 29 181 1.24e-26 99.4
MS.gene78205.t1 AT1G31200 39.370 127 62 5 47 159 3 128 7.26e-18 75.5

Find 43 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAAGGACTCAACATTATA+TGG 0.205970 2.2:+32818409 MS.gene78205:CDS
GAGGAAAAGGAACTTCATTT+TGG 0.223452 2.2:+32819818 MS.gene78205:CDS
CATATAATGTTGAGTCCTTT+TGG 0.242983 2.2:-32818408 None:intergenic
TTGTTAATTATATAAATAAA+TGG 0.263676 2.2:-32818339 None:intergenic
GATGGCTAAAGTTGGGAAAA+AGG 0.285093 2.2:+32819700 MS.gene78205:CDS
TCAAGCTTAGCCTCATCTTT+TGG 0.301147 2.2:-32819779 None:intergenic
CAGAGCTGACACAAGTTTCA+TGG 0.313789 2.2:+32819564 MS.gene78205:CDS
GTTTCATGGTTGGAGGTATC+TGG 0.319576 2.2:+32819578 MS.gene78205:CDS
GTTCCCCAACCGAAACCTTC+TGG 0.324475 2.2:-32819659 None:intergenic
AAAGTAAAAGTAGTAGATAA+AGG 0.334874 2.2:+32819602 MS.gene78205:CDS
ATGAAGTATGGAGTGGAAAA+TGG 0.336655 2.2:+32819846 MS.gene78205:CDS
GATATACGTGTGCAGGTTGA+TGG 0.418940 2.2:+32818369 MS.gene78205:CDS
AAGTAAAAGTAGTAGATAAA+GGG 0.432345 2.2:+32819603 MS.gene78205:CDS
AAAAGGACTCAACATTATAT+GGG 0.432953 2.2:+32818410 MS.gene78205:CDS
GGGGAAATGATCCTCGCTAC+TGG 0.437766 2.2:+32818430 MS.gene78205:CDS
AAGCCAGAAGGTTTCGGTTG+GGG 0.447465 2.2:+32819656 MS.gene78205:CDS
TGAAGTGTCAGGTGAGGAAA+AGG 0.454863 2.2:+32819805 MS.gene78205:CDS
TAAGCCAGAAGGTTTCGGTT+GGG 0.457218 2.2:+32819655 MS.gene78205:CDS
GAAGTTAAGCCAGAAGGTTT+CGG 0.458947 2.2:+32819650 MS.gene78205:CDS
GGGAACCAGCACAGATGTAC+TGG 0.493026 2.2:+32819676 MS.gene78205:CDS
AAGCTTGAAATTGAAGTGTC+AGG 0.504703 2.2:+32819794 MS.gene78205:CDS
GAAATATACAGTGAAATTTG+AGG 0.518771 2.2:+32819625 MS.gene78205:CDS
AAAGGGAACATATAAATATG+AGG 0.525864 2.2:+32819718 MS.gene78205:CDS
GACACAAGTTTCATGGTTGG+AGG 0.541826 2.2:+32819571 MS.gene78205:CDS
GCTGACACAAGTTTCATGGT+TGG 0.548605 2.2:+32819568 MS.gene78205:CDS
CATCACCAGTACATCTGTGC+TGG 0.558599 2.2:-32819681 None:intergenic
CGGAGGCGATATACGTGTGC+AGG 0.566543 2.2:+32818362 MS.gene78205:CDS
AAGTGCGAGATAAAACCAAA+AGG 0.571191 2.2:+32818393 MS.gene78205:CDS
TACTGGTGATGGCTAAAGTT+GGG 0.572904 2.2:+32819693 MS.gene78205:CDS
TGGAGTGGAAAATGGAAAGG+AGG 0.576113 2.2:+32819854 MS.gene78205:CDS
TTAAGCCAGAAGGTTTCGGT+TGG 0.579224 2.2:+32819654 MS.gene78205:CDS
GTACTGGTGATGGCTAAAGT+TGG 0.597259 2.2:+32819692 MS.gene78205:CDS
CAGCACAGATGTACTGGTGA+TGG 0.597825 2.2:+32819682 MS.gene78205:CDS
TTTATTTATATAATTAACAA+CGG 0.607777 2.2:+32818342 MS.gene78205:CDS
GTATGGAGTGGAAAATGGAA+AGG 0.608777 2.2:+32819851 MS.gene78205:CDS
ATATACAGTGAAATTTGAGG+TGG 0.617992 2.2:+32819628 MS.gene78205:CDS
GACAACCATTCCAAAAGATG+AGG 0.618532 2.2:+32819769 MS.gene78205:CDS
GAGGTGGAAGTTAAGCCAGA+AGG 0.632622 2.2:+32819644 MS.gene78205:CDS
ATTTATATAATTAACAACGG+AGG 0.643308 2.2:+32818345 MS.gene78205:CDS
AAAGGACTCAACATTATATG+GGG 0.648494 2.2:+32818411 MS.gene78205:CDS
TGAAATTGAAGTGTCAGGTG+AGG 0.650518 2.2:+32819799 MS.gene78205:CDS
GGATTGTATGAAGTATGGAG+TGG 0.655368 2.2:+32819839 MS.gene78205:CDS
GAGGTAGTGCATCTAGAGTG+TGG 0.663909 2.2:+32819737 MS.gene78205:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG + chr2.2:32819307-32819326 MS.gene78205:intron 10.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr2.2:32819306-32819325 MS.gene78205:intron 10.0%
!! TATGTATATGTATAAATTAA+GGG + chr2.2:32818764-32818783 MS.gene78205:intron 10.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr2.2:32819308-32819327 MS.gene78205:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr2.2:32819372-32819391 MS.gene78205:intron 15.0%
!! GTATGTATATGTATAAATTA+AGG + chr2.2:32818763-32818782 MS.gene78205:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr2.2:32819371-32819390 MS.gene78205:intron 15.0%
!!! AAATCGAAGAAATTTTTTAA+AGG + chr2.2:32819164-32819183 MS.gene78205:intron 15.0%
!!! AATCGAAGAAATTTTTTAAA+GGG + chr2.2:32819165-32819184 MS.gene78205:intron 15.0%
!!! TATGAATTTTATATTTTTGG+CGG - chr2.2:32819034-32819053 None:intergenic 15.0%
!! AAAAAACAACAAATATCAAG+AGG - chr2.2:32818565-32818584 None:intergenic 20.0%
!! AAAGTAAAAGTAGTAGATAA+AGG + chr2.2:32819602-32819621 MS.gene78205:CDS 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr2.2:32819331-32819350 MS.gene78205:intron 20.0%
!! AAGTAAAAGTAGTAGATAAA+GGG + chr2.2:32819603-32819622 MS.gene78205:CDS 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr2.2:32819404-32819423 MS.gene78205:intron 20.0%
!! ATTTATATAATTAACAACGG+AGG + chr2.2:32818345-32818364 MS.gene78205:CDS 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr2.2:32819405-32819424 MS.gene78205:intron 20.0%
!!! ACATTTTATATACTGATTTG+AGG + chr2.2:32818623-32818642 MS.gene78205:intron 20.0%
!!! CTAAAAACATATTTAAAGCA+TGG + chr2.2:32819220-32819239 MS.gene78205:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr2.2:32819441-32819460 MS.gene78205:intron 20.0%
!!! TGTTGTTTTTTTTTTTGTTG+AGG + chr2.2:32818574-32818593 MS.gene78205:intron 20.0%
! AAAAGGACTCAACATTATAT+GGG + chr2.2:32818410-32818429 MS.gene78205:CDS 25.0%
! AAAGGGAACATATAAATATG+AGG + chr2.2:32819718-32819737 MS.gene78205:CDS 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr2.2:32819330-32819349 MS.gene78205:intron 25.0%
! GAAATATACAGTGAAATTTG+AGG + chr2.2:32819625-32819644 MS.gene78205:CDS 25.0%
! TATACATACATATGTGAAGA+AGG - chr2.2:32818752-32818771 None:intergenic 25.0%
! TTATAGATACTAAAAGTCGA+AGG - chr2.2:32818851-32818870 None:intergenic 25.0%
!! AAGTCGAAGGAAAATTTTAT+AGG - chr2.2:32818838-32818857 None:intergenic 25.0%
!! ACATGAGTTGACTTAAAAAT+AGG - chr2.2:32818898-32818917 None:intergenic 25.0%
!! AGTCGAAGGAAAATTTTATA+GGG - chr2.2:32818837-32818856 None:intergenic 25.0%
!! ATGAGTTGACTTAAAAATAG+GGG - chr2.2:32818896-32818915 None:intergenic 25.0%
!! ATTTTGGATTGTATGAAGTA+TGG + chr2.2:32819834-32819853 MS.gene78205:CDS 25.0%
!! CATGAGTTGACTTAAAAATA+GGG - chr2.2:32818897-32818916 None:intergenic 25.0%
!! TACATATGAGTTTGCTTAAA+AGG + chr2.2:32819102-32819121 MS.gene78205:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr2.2:32819440-32819459 MS.gene78205:intron 25.0%
AAAGGACTCAACATTATATG+GGG + chr2.2:32818411-32818430 MS.gene78205:CDS 30.0%
ATATACAGTGAAATTTGAGG+TGG + chr2.2:32819628-32819647 MS.gene78205:CDS 30.0%
CAAAAGGACTCAACATTATA+TGG + chr2.2:32818409-32818428 MS.gene78205:CDS 30.0%
TGATTAGATAGAGTTCTACA+TGG - chr2.2:32818536-32818555 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr2.2:32819315-32819334 MS.gene78205:intron 30.0%
TGTCTTTCACAAATATCAAG+AGG - chr2.2:32818606-32818625 None:intergenic 30.0%
TTAAGGGTTAAATACGTGTT+TGG + chr2.2:32818780-32818799 MS.gene78205:intron 30.0%
TTATGTGCACACATTATGTT+AGG + chr2.2:32818696-32818715 MS.gene78205:intron 30.0%
! AAAAATTCCATCTTCGCTTT+TGG - chr2.2:32819133-32819152 None:intergenic 30.0%
! CATATAATGTTGAGTCCTTT+TGG - chr2.2:32818411-32818430 None:intergenic 30.0%
! TATGAGTTTGCTTAAAAGGA+TGG + chr2.2:32819106-32819125 MS.gene78205:intron 30.0%
! TTGAAATGGGTCAGTTATTT+TGG + chr2.2:32819418-32819437 MS.gene78205:intron 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG + chr2.2:32819419-32819438 MS.gene78205:intron 30.0%
AAGTGCGAGATAAAACCAAA+AGG + chr2.2:32818393-32818412 MS.gene78205:CDS 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr2.2:32819454-32819473 MS.gene78205:intron 35.0%
ATGAAGTATGGAGTGGAAAA+TGG + chr2.2:32819846-32819865 MS.gene78205:CDS 35.0%
ATGGCTAAAGTTGGGAAAAA+GGG + chr2.2:32819701-32819720 MS.gene78205:CDS 35.0%
CAAAGTAGGTCAATTATACC+GGG - chr2.2:32819271-32819290 None:intergenic 35.0%
CCAAAGTAGGTCAATTATAC+CGG - chr2.2:32819272-32819291 None:intergenic 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - chr2.2:32819285-32819304 None:intergenic 35.0%
! AAGCTTGAAATTGAAGTGTC+AGG + chr2.2:32819794-32819813 MS.gene78205:CDS 35.0%
! GAGGAAAAGGAACTTCATTT+TGG + chr2.2:32819818-32819837 MS.gene78205:CDS 35.0%
! TCTATTATTTTCCAGTAGCG+AGG - chr2.2:32818444-32818463 None:intergenic 35.0%
!!! TCTTTTGGAATGGTTGTCTT+AGG - chr2.2:32819767-32819786 None:intergenic 35.0%
CCGGTATAATTGACCTACTT+TGG + chr2.2:32819269-32819288 MS.gene78205:intron 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr2.2:32819455-32819474 MS.gene78205:intron 40.0%
GAAGTTAAGCCAGAAGGTTT+CGG + chr2.2:32819650-32819669 MS.gene78205:CDS 40.0%
GACAACCATTCCAAAAGATG+AGG + chr2.2:32819769-32819788 MS.gene78205:CDS 40.0%
GATGGCTAAAGTTGGGAAAA+AGG + chr2.2:32819700-32819719 MS.gene78205:CDS 40.0%
GGATTGTATGAAGTATGGAG+TGG + chr2.2:32819839-32819858 MS.gene78205:CDS 40.0%
GTATGGAGTGGAAAATGGAA+AGG + chr2.2:32819851-32819870 MS.gene78205:CDS 40.0%
GTGCACACATTATGTTAGGA+TGG + chr2.2:32818700-32818719 MS.gene78205:intron 40.0%
! TCAAGCTTAGCCTCATCTTT+TGG - chr2.2:32819782-32819801 None:intergenic 40.0%
! TGAAATTGAAGTGTCAGGTG+AGG + chr2.2:32819799-32819818 MS.gene78205:CDS 40.0%
!! CTTAGCCTCATCTTTTGGAA+TGG - chr2.2:32819777-32819796 None:intergenic 40.0%
!! TACTGGTGATGGCTAAAGTT+GGG + chr2.2:32819693-32819712 MS.gene78205:CDS 40.0%
CAGAGCTGACACAAGTTTCA+TGG + chr2.2:32819564-32819583 MS.gene78205:CDS 45.0%
GACACAAGTTTCATGGTTGG+AGG + chr2.2:32819571-32819590 MS.gene78205:CDS 45.0%
GCTGACACAAGTTTCATGGT+TGG + chr2.2:32819568-32819587 MS.gene78205:CDS 45.0%
GTAGGTCAATTATACCGGGA+CGG - chr2.2:32819267-32819286 None:intergenic 45.0%
TAAGCCAGAAGGTTTCGGTT+GGG + chr2.2:32819655-32819674 MS.gene78205:CDS 45.0%
TGGAGTGGAAAATGGAAAGG+AGG + chr2.2:32819854-32819873 MS.gene78205:CDS 45.0%
TTAAGCCAGAAGGTTTCGGT+TGG + chr2.2:32819654-32819673 MS.gene78205:CDS 45.0%
! GATATACGTGTGCAGGTTGA+TGG + chr2.2:32818369-32818388 MS.gene78205:CDS 45.0%
! TGAAGTGTCAGGTGAGGAAA+AGG + chr2.2:32819805-32819824 MS.gene78205:CDS 45.0%
!! GTACTGGTGATGGCTAAAGT+TGG + chr2.2:32819692-32819711 MS.gene78205:CDS 45.0%
!! GTTTCATGGTTGGAGGTATC+TGG + chr2.2:32819578-32819597 MS.gene78205:CDS 45.0%
!! TTGTTAATTATATAAATAAA+TGG - chr2.2:32818342-32818361 None:intergenic 5.0%
!! TTTATTTATATAATTAACAA+CGG + chr2.2:32818342-32818361 MS.gene78205:CDS 5.0%
!!! AAATATGAATTTTATATTTT+TGG - chr2.2:32819037-32819056 None:intergenic 5.0%
AAATACTACTCCCTCCCTCC+CGG - chr2.2:32819476-32819495 None:intergenic 50.0%
AAGCCAGAAGGTTTCGGTTG+GGG + chr2.2:32819656-32819675 MS.gene78205:CDS 50.0%
AATGGGTCAGTTATTCCGGG+AGG + chr2.2:32819458-32819477 MS.gene78205:intron 50.0%
ATGGGTCAGTTATTCCGGGA+GGG + chr2.2:32819459-32819478 MS.gene78205:intron 50.0%
CATCACCAGTACATCTGTGC+TGG - chr2.2:32819684-32819703 None:intergenic 50.0%
GAGGTGGAAGTTAAGCCAGA+AGG + chr2.2:32819644-32819663 MS.gene78205:CDS 50.0%
GGATGGACCAAAAGCGAAGA+TGG + chr2.2:32819123-32819142 MS.gene78205:intron 50.0%
GTCTAATACTACCTCCGTCC+CGG + chr2.2:32819250-32819269 MS.gene78205:intron 50.0%
! CAGCACAGATGTACTGGTGA+TGG + chr2.2:32819682-32819701 MS.gene78205:CDS 50.0%
! GAGGTAGTGCATCTAGAGTG+TGG + chr2.2:32819737-32819756 MS.gene78205:CDS 50.0%
GGGAACCAGCACAGATGTAC+TGG + chr2.2:32819676-32819695 MS.gene78205:CDS 55.0%
GGGGAAATGATCCTCGCTAC+TGG + chr2.2:32818430-32818449 MS.gene78205:CDS 55.0%
GGTCAATTATACCGGGACGG+AGG - chr2.2:32819264-32819283 None:intergenic 55.0%
GTCAGTTATTCCGGGAGGGA+GGG + chr2.2:32819463-32819482 MS.gene78205:intron 55.0%
GTTCCCCAACCGAAACCTTC+TGG - chr2.2:32819662-32819681 None:intergenic 55.0%
CGGAGGCGATATACGTGTGC+AGG + chr2.2:32818362-32818381 MS.gene78205:CDS 60.0%
GGTCAGTTATTCCGGGAGGG+AGG + chr2.2:32819462-32819481 MS.gene78205:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 32818333 32819916 32818333 ID=MS.gene78205
chr2.2 mRNA 32818333 32819916 32818333 ID=MS.gene78205.t1;Parent=MS.gene78205
chr2.2 exon 32818333 32818463 32818333 ID=MS.gene78205.t1.exon1;Parent=MS.gene78205.t1
chr2.2 CDS 32818333 32818463 32818333 ID=cds.MS.gene78205.t1;Parent=MS.gene78205.t1
chr2.2 exon 32819556 32819916 32819556 ID=MS.gene78205.t1.exon2;Parent=MS.gene78205.t1
chr2.2 CDS 32819556 32819916 32819556 ID=cds.MS.gene78205.t1;Parent=MS.gene78205.t1
Gene Sequence

>MS.gene78205

ATGCTGCCATTTATTTATATAATTAACAACGGAGGCGATATACGTGTGCAGGTTGATGGCAAGTGCGAGATAAAACCAAAAGGACTCAACATTATATGGGGAAATGATCCTCGCTACTGGAAAATAATAGAGTAAGTTGCATTCATATATTGTTATTACAATGAAAATGATAACTTATTGATTTTATTTGCATACACAAACCATGTAGAACTCTATCTAATCAAATGTACCTCTTGATATTTGTTGTTTTTTTTTTTGTTGAGGAAAGTACCTCTTGATATTTGTGAAAGACATTTTATATACTGATTTGAGGAATGATATGATATTATAGTATGATTTGTCTGAATACATGAGCAAAATCTATTATGTGCACACATTATGTTAGGATGGTAATTAAATTATATAATTTTATTTTTCCTTCTTCACATATGTATGTATATGTATAAATTAAGGGTTAAATACGTGTTTGGTCTCTATAAATATATCGATTTTTTTTTAAGTCCCTATAAAATTTTCCTTCGACTTTTAGTATCTATAAAATTTTCTGTCATCACTTTTGACCCCTATTTTTAAGTCAACTCATGTGTAGCTTTTGTATTTTTTAATAAAATTGTGCAAAAATATGTAGAATATTGTAAAAATCTCTTTAAAAGAAATTTGAATTTTTAACAAAATATAAATTAAATATGAATTTTTAACCGCCAAAAATATAAAATTCATATTTAATTTATGTTTTGTTAAAAAATTTATAAAAAAAAAAAAAGAATTTTACATATGAGTTTGCTTAAAAGGATGGACCAAAAGCGAAGATGGAATTTTTTTTAAGACTAAAAATCGAAGAAATTTTTTAAAGGGATTAAAATGACAAGTTTGTATATTTATAAGAACTAAAAACATATTTAAAGCATGGATTAATTGTCTAATACTACCTCCGTCCCGGTATAATTGACCTACTTTGGTTTTTCACACAGATTAAGAAAAATGTATAAATAAAGGGGAAAGTGGAATATATTGTATTGGGAATGATAAAAGTAGTATTATTTAGTGTTATATTGTATTGGGAATGATGAAAGTTGTATTGAAAATTGAAATGGGTCAGTTATTTTGGGACACTTTTTTTTAGAAAATGGGTCAGTTATTCCGGGAGGGAGGGAGTAGTATTTTTTTTGATAGAGATGTGTTAATTAAATAATTAATGTTTCTATATGCATGCATTATTTCAGCGAAGTTGCAGAGCTGACACAAGTTTCATGGTTGGAGGTATCTGGTAAAGTAAAAGTAGTAGATAAAGGGAAATATACAGTGAAATTTGAGGTGGAAGTTAAGCCAGAAGGTTTCGGTTGGGGAACCAGCACAGATGTACTGGTGATGGCTAAAGTTGGGAAAAAGGGAACATATAAATATGAGGTAGTGCATCTAGAGTGTGGTACTCCTAAGACAACCATTCCAAAAGATGAGGCTAAGCTTGAAATTGAAGTGTCAGGTGAGGAAAAGGAACTTCATTTTGGATTGTATGAAGTATGGAGTGGAAAATGGAAAGGAGGTTTGAAAATCTACAATGCAGTAGTTGCTAAGACGCCTTAA

Protein sequence

>MS.gene78205.t1

MLPFIYIINNGGDIRVQVDGKCEIKPKGLNIIWGNDPRYWKIIDEVAELTQVSWLEVSGKVKVVDKGKYTVKFEVEVKPEGFGWGTSTDVLVMAKVGKKGTYKYEVVHLECGTPKTTIPKDEAKLEIEVSGEEKELHFGLYEVWSGKWKGGLKIYNAVVAKTP