AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84401


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84401.t1 MTR_0026s0010 73.944 142 34 2 22 161 25 165 8.97e-65 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84401.t1 AT1G10155 41.558 154 71 6 24 159 29 181 1.24e-26 99.4
MS.gene84401.t1 AT1G31200 39.370 127 62 5 47 159 3 128 7.26e-18 75.5

Find 43 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAAGGACTCAACATTATA+TGG 0.205970 2.4:+37148578 MS.gene84401:CDS
GAGGAAAAGGAACTTCATTT+TGG 0.223452 2.4:+37149750 MS.gene84401:CDS
CATATAATGTTGAGTCCTTT+TGG 0.242983 2.4:-37148577 None:intergenic
TTGTTAATTATATAAATAAA+TGG 0.263676 2.4:-37148508 None:intergenic
GATGGCTAAAGTTGGGAAAA+AGG 0.285093 2.4:+37149632 MS.gene84401:CDS
TCAAGCTTAGCCTCATCTTT+TGG 0.301147 2.4:-37149711 None:intergenic
CAGAGCTGACACAAGTTTCA+TGG 0.313789 2.4:+37149496 MS.gene84401:CDS
GTTTCATGGTTGGAGGTATC+TGG 0.319576 2.4:+37149510 MS.gene84401:CDS
GTTCCCCAACCGAAACCTTC+TGG 0.324475 2.4:-37149591 None:intergenic
AAAGTAAAAGTAGTAGATAA+AGG 0.334874 2.4:+37149534 MS.gene84401:CDS
ATGAAGTATGGAGTGGAAAA+TGG 0.336655 2.4:+37149778 MS.gene84401:CDS
GATATACGTGTGCAGGTTGA+TGG 0.418940 2.4:+37148538 MS.gene84401:CDS
AAGTAAAAGTAGTAGATAAA+GGG 0.432345 2.4:+37149535 MS.gene84401:CDS
AAAAGGACTCAACATTATAT+GGG 0.432953 2.4:+37148579 MS.gene84401:CDS
GGGGAAATGATCCTCGCTAC+TGG 0.437766 2.4:+37148599 MS.gene84401:CDS
AAGCCAGAAGGTTTCGGTTG+GGG 0.447465 2.4:+37149588 MS.gene84401:CDS
TGAAGTGTCAGGTGAGGAAA+AGG 0.454863 2.4:+37149737 MS.gene84401:CDS
TAAGCCAGAAGGTTTCGGTT+GGG 0.457218 2.4:+37149587 MS.gene84401:CDS
GAAGTTAAGCCAGAAGGTTT+CGG 0.458947 2.4:+37149582 MS.gene84401:CDS
GGGAACCAGCACAGATGTAC+TGG 0.493026 2.4:+37149608 MS.gene84401:CDS
AAGCTTGAAATTGAAGTGTC+AGG 0.504703 2.4:+37149726 MS.gene84401:CDS
GAAATATACAGTGAAATTTG+AGG 0.518771 2.4:+37149557 MS.gene84401:CDS
AAAGGGAACATATAAATATG+AGG 0.525864 2.4:+37149650 MS.gene84401:CDS
GACACAAGTTTCATGGTTGG+AGG 0.541826 2.4:+37149503 MS.gene84401:CDS
GCTGACACAAGTTTCATGGT+TGG 0.548605 2.4:+37149500 MS.gene84401:CDS
CATCACCAGTACATCTGTGC+TGG 0.558599 2.4:-37149613 None:intergenic
CGGAGGCGATATACGTGTGC+AGG 0.566543 2.4:+37148531 MS.gene84401:CDS
AAGTGCGAGATAAAACCAAA+AGG 0.571191 2.4:+37148562 MS.gene84401:CDS
TACTGGTGATGGCTAAAGTT+GGG 0.572904 2.4:+37149625 MS.gene84401:CDS
TGGAGTGGAAAATGGAAAGG+AGG 0.576113 2.4:+37149786 MS.gene84401:CDS
TTAAGCCAGAAGGTTTCGGT+TGG 0.579224 2.4:+37149586 MS.gene84401:CDS
GTACTGGTGATGGCTAAAGT+TGG 0.597259 2.4:+37149624 MS.gene84401:CDS
CAGCACAGATGTACTGGTGA+TGG 0.597825 2.4:+37149614 MS.gene84401:CDS
TTTATTTATATAATTAACAA+CGG 0.607777 2.4:+37148511 MS.gene84401:CDS
GTATGGAGTGGAAAATGGAA+AGG 0.608777 2.4:+37149783 MS.gene84401:CDS
ATATACAGTGAAATTTGAGG+TGG 0.617992 2.4:+37149560 MS.gene84401:CDS
GACAACCATTCCAAAAGATG+AGG 0.618532 2.4:+37149701 MS.gene84401:CDS
GAGGTGGAAGTTAAGCCAGA+AGG 0.632622 2.4:+37149576 MS.gene84401:CDS
ATTTATATAATTAACAACGG+AGG 0.643308 2.4:+37148514 MS.gene84401:CDS
AAAGGACTCAACATTATATG+GGG 0.648494 2.4:+37148580 MS.gene84401:CDS
TGAAATTGAAGTGTCAGGTG+AGG 0.650518 2.4:+37149731 MS.gene84401:CDS
GGATTGTATGAAGTATGGAG+TGG 0.655368 2.4:+37149771 MS.gene84401:CDS
GAGGTAGTGCATCTAGAGTG+TGG 0.663909 2.4:+37149669 MS.gene84401:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATGTATGTATAAATTA+AGG + chr2.4:37148926-37148945 MS.gene84401:intron 15.0%
!! CATATGTATGTATAAATTAA+GGG + chr2.4:37148927-37148946 MS.gene84401:intron 15.0%
!!! AAATCGAAGAAATTTTTTAA+AGG + chr2.4:37149325-37149344 MS.gene84401:intron 15.0%
!!! AATCGAAGAAATTTTTTAAA+GGG + chr2.4:37149326-37149345 MS.gene84401:intron 15.0%
!!! TATGAATTTTATATTTTTGG+CGG - chr2.4:37149197-37149216 None:intergenic 15.0%
!! AAAAAACAACAAATATCAAG+AGG - chr2.4:37148734-37148753 None:intergenic 20.0%
!! AAAGTAAAAGTAGTAGATAA+AGG + chr2.4:37149534-37149553 MS.gene84401:CDS 20.0%
!! AAGTAAAAGTAGTAGATAAA+GGG + chr2.4:37149535-37149554 MS.gene84401:CDS 20.0%
!! ATTTATATAATTAACAACGG+AGG + chr2.4:37148514-37148533 MS.gene84401:CDS 20.0%
!!! ACATTTTATATACTGATTTG+AGG + chr2.4:37148792-37148811 MS.gene84401:intron 20.0%
!!! CTAAAAACATATTTAAAGCA+TGG + chr2.4:37149381-37149400 MS.gene84401:intron 20.0%
!!! TGTTGTTTTTTTTTTTGTTG+AGG + chr2.4:37148743-37148762 MS.gene84401:intron 20.0%
! AAAAGGACTCAACATTATAT+GGG + chr2.4:37148579-37148598 MS.gene84401:CDS 25.0%
! AAAGGGAACATATAAATATG+AGG + chr2.4:37149650-37149669 MS.gene84401:CDS 25.0%
! GAAATATACAGTGAAATTTG+AGG + chr2.4:37149557-37149576 MS.gene84401:CDS 25.0%
! TATACATACATATGTGAAGA+AGG - chr2.4:37148921-37148940 None:intergenic 25.0%
! TTATAGATACTAAAAGTCGA+AGG - chr2.4:37149014-37149033 None:intergenic 25.0%
!! AAGTCGAAGGAAAATTTTAT+AGG - chr2.4:37149001-37149020 None:intergenic 25.0%
!! ACATGAGTTGACTTAAAAAT+AGG - chr2.4:37149061-37149080 None:intergenic 25.0%
!! AGTCGAAGGAAAATTTTATA+GGG - chr2.4:37149000-37149019 None:intergenic 25.0%
!! ATGAGTTGACTTAAAAATAG+GGG - chr2.4:37149059-37149078 None:intergenic 25.0%
!! ATTTTGGATTGTATGAAGTA+TGG + chr2.4:37149766-37149785 MS.gene84401:CDS 25.0%
!! CATGAGTTGACTTAAAAATA+GGG - chr2.4:37149060-37149079 None:intergenic 25.0%
!! TACATATGAGTTTGCTTAAA+AGG + chr2.4:37149263-37149282 MS.gene84401:intron 25.0%
AAAGGACTCAACATTATATG+GGG + chr2.4:37148580-37148599 MS.gene84401:CDS 30.0%
ATATACAGTGAAATTTGAGG+TGG + chr2.4:37149560-37149579 MS.gene84401:CDS 30.0%
CAAAAGGACTCAACATTATA+TGG + chr2.4:37148578-37148597 MS.gene84401:CDS 30.0%
TGATTAGATAGAGTTCTACA+TGG - chr2.4:37148705-37148724 None:intergenic 30.0%
TGTCTTTCACAAATATCAAG+AGG - chr2.4:37148775-37148794 None:intergenic 30.0%
TTAAGGGTTAAATACGTGTT+TGG + chr2.4:37148943-37148962 MS.gene84401:intron 30.0%
TTATGTGCACACATTATGTT+AGG + chr2.4:37148865-37148884 MS.gene84401:intron 30.0%
! AAAAATTCCATCTTCGCTTT+TGG - chr2.4:37149294-37149313 None:intergenic 30.0%
! CATATAATGTTGAGTCCTTT+TGG - chr2.4:37148580-37148599 None:intergenic 30.0%
! TATGAGTTTGCTTAAAAGGA+TGG + chr2.4:37149267-37149286 MS.gene84401:intron 30.0%
AAGTGCGAGATAAAACCAAA+AGG + chr2.4:37148562-37148581 MS.gene84401:CDS 35.0%
ATGAAGTATGGAGTGGAAAA+TGG + chr2.4:37149778-37149797 MS.gene84401:CDS 35.0%
ATGGCTAAAGTTGGGAAAAA+GGG + chr2.4:37149633-37149652 MS.gene84401:CDS 35.0%
! AAGCTTGAAATTGAAGTGTC+AGG + chr2.4:37149726-37149745 MS.gene84401:CDS 35.0%
! GAGGAAAAGGAACTTCATTT+TGG + chr2.4:37149750-37149769 MS.gene84401:CDS 35.0%
! TCTATTATTTTCCAGTAGCG+AGG - chr2.4:37148613-37148632 None:intergenic 35.0%
!!! TCTTTTGGAATGGTTGTCTT+AGG - chr2.4:37149699-37149718 None:intergenic 35.0%
GAAGTTAAGCCAGAAGGTTT+CGG + chr2.4:37149582-37149601 MS.gene84401:CDS 40.0%
GACAACCATTCCAAAAGATG+AGG + chr2.4:37149701-37149720 MS.gene84401:CDS 40.0%
GATGGCTAAAGTTGGGAAAA+AGG + chr2.4:37149632-37149651 MS.gene84401:CDS 40.0%
GGATTGTATGAAGTATGGAG+TGG + chr2.4:37149771-37149790 MS.gene84401:CDS 40.0%
GTATGGAGTGGAAAATGGAA+AGG + chr2.4:37149783-37149802 MS.gene84401:CDS 40.0%
GTGCACACATTATGTTAGGA+TGG + chr2.4:37148869-37148888 MS.gene84401:intron 40.0%
! TCAAGCTTAGCCTCATCTTT+TGG - chr2.4:37149714-37149733 None:intergenic 40.0%
! TGAAATTGAAGTGTCAGGTG+AGG + chr2.4:37149731-37149750 MS.gene84401:CDS 40.0%
!! CTTAGCCTCATCTTTTGGAA+TGG - chr2.4:37149709-37149728 None:intergenic 40.0%
!! TACTGGTGATGGCTAAAGTT+GGG + chr2.4:37149625-37149644 MS.gene84401:CDS 40.0%
CAGAGCTGACACAAGTTTCA+TGG + chr2.4:37149496-37149515 MS.gene84401:CDS 45.0%
GACACAAGTTTCATGGTTGG+AGG + chr2.4:37149503-37149522 MS.gene84401:CDS 45.0%
GCTGACACAAGTTTCATGGT+TGG + chr2.4:37149500-37149519 MS.gene84401:CDS 45.0%
TAAGCCAGAAGGTTTCGGTT+GGG + chr2.4:37149587-37149606 MS.gene84401:CDS 45.0%
TGGAGTGGAAAATGGAAAGG+AGG + chr2.4:37149786-37149805 MS.gene84401:CDS 45.0%
TTAAGCCAGAAGGTTTCGGT+TGG + chr2.4:37149586-37149605 MS.gene84401:CDS 45.0%
! GATATACGTGTGCAGGTTGA+TGG + chr2.4:37148538-37148557 MS.gene84401:CDS 45.0%
! TGAAGTGTCAGGTGAGGAAA+AGG + chr2.4:37149737-37149756 MS.gene84401:CDS 45.0%
!! GTACTGGTGATGGCTAAAGT+TGG + chr2.4:37149624-37149643 MS.gene84401:CDS 45.0%
!! GTTTCATGGTTGGAGGTATC+TGG + chr2.4:37149510-37149529 MS.gene84401:CDS 45.0%
!! TTGTTAATTATATAAATAAA+TGG - chr2.4:37148511-37148530 None:intergenic 5.0%
!! TTTATTTATATAATTAACAA+CGG + chr2.4:37148511-37148530 MS.gene84401:CDS 5.0%
!!! AAATATGAATTTTATATTTT+TGG - chr2.4:37149200-37149219 None:intergenic 5.0%
AAGCCAGAAGGTTTCGGTTG+GGG + chr2.4:37149588-37149607 MS.gene84401:CDS 50.0%
CATCACCAGTACATCTGTGC+TGG - chr2.4:37149616-37149635 None:intergenic 50.0%
GAGGTGGAAGTTAAGCCAGA+AGG + chr2.4:37149576-37149595 MS.gene84401:CDS 50.0%
GGATGGACCAAAAGCGAAGA+TGG + chr2.4:37149284-37149303 MS.gene84401:intron 50.0%
! CAGCACAGATGTACTGGTGA+TGG + chr2.4:37149614-37149633 MS.gene84401:CDS 50.0%
! GAGGTAGTGCATCTAGAGTG+TGG + chr2.4:37149669-37149688 MS.gene84401:CDS 50.0%
GGGAACCAGCACAGATGTAC+TGG + chr2.4:37149608-37149627 MS.gene84401:CDS 55.0%
GGGGAAATGATCCTCGCTAC+TGG + chr2.4:37148599-37148618 MS.gene84401:CDS 55.0%
GTTCCCCAACCGAAACCTTC+TGG - chr2.4:37149594-37149613 None:intergenic 55.0%
CGGAGGCGATATACGTGTGC+AGG + chr2.4:37148531-37148550 MS.gene84401:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 37148502 37149848 37148502 ID=MS.gene84401
chr2.4 mRNA 37148502 37149848 37148502 ID=MS.gene84401.t1;Parent=MS.gene84401
chr2.4 exon 37148502 37148632 37148502 ID=MS.gene84401.t1.exon1;Parent=MS.gene84401.t1
chr2.4 CDS 37148502 37148632 37148502 ID=cds.MS.gene84401.t1;Parent=MS.gene84401.t1
chr2.4 exon 37149488 37149848 37149488 ID=MS.gene84401.t1.exon2;Parent=MS.gene84401.t1
chr2.4 CDS 37149488 37149848 37149488 ID=cds.MS.gene84401.t1;Parent=MS.gene84401.t1
Gene Sequence

>MS.gene84401

ATGCTGCCATTTATTTATATAATTAACAACGGAGGCGATATACGTGTGCAGGTTGATGGCAAGTGCGAGATAAAACCAAAAGGACTCAACATTATATGGGGAAATGATCCTCGCTACTGGAAAATAATAGAGTAAGTTGCATTCATATATTGTTATTACAATGAAAATGATAACTTATTGATTTTATTTGCATACACAAACCATGTAGAACTCTATCTAATCAAATGTACCTCTTGATATTTGTTGTTTTTTTTTTTGTTGAGGAAAGTACCTCTTGATATTTGTGAAAGACATTTTATATACTGATTTGAGGAATGATATGATATTATAGTATGATTTGTCTGAATACATGAGCAAAATCTATTATGTGCACACATTATGTTAGGATGGTAATTAAATTATATAATTTTATTTTTCCTTCTTCACATATGTATGTATAAATTAAGGGTTAAATACGTGTTTGGTCTCTATAAATATATCGATTTTTTTTTAAGTCCCTATAAAATTTTCCTTCGACTTTTAGTATCTATAAAATTTTCTGTCATCACTTTTGACCCCTATTTTTAAGTCAACTCATGTGTAGCTTTTGTATTTTTTAATAAAATTGTGCAAAAATATGTAGAATATTGTAAAAATCTCTTTAAAAGAAATTAGAATTTTTAACAAAATATAAATTAAATATGAATTTTTAACCGCCAAAAATATAAAATTCATATTTAATTTATGTTTTGTTAAAAAATTTATAAAAAAAAAAAGAATTTTACATATGAGTTTGCTTAAAAGGATGGACCAAAAGCGAAGATGGAATTTTTTTTAAGACTAAAAATCGAAGAAATTTTTTAAAGGGATTAAAATGACAAGTTTGTATATTTATAAGAACTAAAAACATATTTAAAGCATGGATTAATTGTCTAATAGTAGTATTTTTTTTGATAGAGATGTGTTAATTAAATAATTAATGTTTCTATATGCATGCATTATTTCAGCGAAGTTGCAGAGCTGACACAAGTTTCATGGTTGGAGGTATCTGGTAAAGTAAAAGTAGTAGATAAAGGGAAATATACAGTGAAATTTGAGGTGGAAGTTAAGCCAGAAGGTTTCGGTTGGGGAACCAGCACAGATGTACTGGTGATGGCTAAAGTTGGGAAAAAGGGAACATATAAATATGAGGTAGTGCATCTAGAGTGTGGTACTCCTAAGACAACCATTCCAAAAGATGAGGCTAAGCTTGAAATTGAAGTGTCAGGTGAGGAAAAGGAACTTCATTTTGGATTGTATGAAGTATGGAGTGGAAAATGGAAAGGAGGTTTGAAAATCTACAATGCAGTAGTTGCTAAGACGCCTTAA

Protein sequence

>MS.gene84401.t1

MLPFIYIINNGGDIRVQVDGKCEIKPKGLNIIWGNDPRYWKIIDEVAELTQVSWLEVSGKVKVVDKGKYTVKFEVEVKPEGFGWGTSTDVLVMAKVGKKGTYKYEVVHLECGTPKTTIPKDEAKLEIEVSGEEKELHFGLYEVWSGKWKGGLKIYNAVVAKTP