Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053520.t1 | XP_003607955.1 | 100 | 73 | 0 | 0 | 1 | 73 | 98 | 170 | 4.80E-33 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053520.t1 | Q9SEU7 | 67.1 | 73 | 24 | 0 | 1 | 73 | 101 | 173 | 2.7e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053520.t1 | G7JL65 | 100.0 | 73 | 0 | 0 | 1 | 73 | 98 | 170 | 3.5e-33 | 149.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052207 | MS.gene053520 | 0.816143 | 6.44E-52 | -1.69E-46 |
| MS.gene053520 | MS.gene071772 | 0.831838 | 1.33E-55 | -1.69E-46 |
| MS.gene053520 | MS.gene27924 | 0.802112 | 6.55E-49 | -1.69E-46 |
| MS.gene053520 | MS.gene59498 | 0.811021 | 8.62E-51 | -1.69E-46 |
| MS.gene053520 | MS.gene64799 | 0.811491 | 6.82E-51 | -1.69E-46 |
| MS.gene053520 | MS.gene70487 | 0.80136 | 9.34E-49 | -1.69E-46 |
| MS.gene053520 | MS.gene75475 | 0.818008 | 2.45E-52 | -1.69E-46 |
| MS.gene053520 | MS.gene79217 | 0.808691 | 2.73E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053520.t1 | MTR_4g085880 | 100.000 | 73 | 0 | 0 | 1 | 73 | 98 | 170 | 5.82e-50 | 154 |
| MS.gene053520.t1 | MTR_2g079420 | 52.113 | 71 | 34 | 0 | 1 | 71 | 103 | 173 | 3.12e-24 | 89.4 |
| MS.gene053520.t1 | MTR_3g089065 | 45.070 | 71 | 39 | 0 | 1 | 71 | 103 | 173 | 1.39e-20 | 80.1 |
| MS.gene053520.t1 | MTR_8g059015 | 45.070 | 71 | 39 | 0 | 1 | 71 | 106 | 176 | 2.30e-20 | 79.7 |
| MS.gene053520.t1 | MTR_2g079400 | 40.625 | 64 | 38 | 0 | 8 | 71 | 74 | 137 | 6.59e-14 | 62.0 |
| MS.gene053520.t1 | MTR_2g079340 | 36.232 | 69 | 44 | 0 | 3 | 71 | 71 | 139 | 9.69e-14 | 62.0 |
| MS.gene053520.t1 | MTR_2g079360 | 35.821 | 67 | 43 | 0 | 2 | 68 | 81 | 147 | 4.26e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053520.t1 | AT2G15570 | 67.123 | 73 | 24 | 0 | 1 | 73 | 101 | 173 | 1.34e-33 | 113 |
| MS.gene053520.t1 | AT2G15570 | 67.123 | 73 | 24 | 0 | 1 | 73 | 102 | 174 | 1.35e-33 | 113 |
| MS.gene053520.t1 | AT3G15360 | 40.845 | 71 | 42 | 0 | 1 | 71 | 121 | 191 | 1.42e-18 | 75.5 |
| MS.gene053520.t1 | AT1G03680 | 33.803 | 71 | 47 | 0 | 1 | 71 | 109 | 179 | 2.94e-14 | 63.9 |
| MS.gene053520.t1 | AT4G03520 | 28.169 | 71 | 51 | 0 | 1 | 71 | 1 | 71 | 6.30e-14 | 60.5 |
| MS.gene053520.t1 | AT4G03520 | 28.169 | 71 | 51 | 0 | 1 | 71 | 115 | 185 | 1.38e-12 | 59.7 |
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCAAATTGCTGAAGAATA+TGG | 0.258338 | 4.3:+26902997 | MS.gene053520:CDS |
| TGCTGAAGAATATGGAATTA+AGG | 0.337886 | 4.3:+26903005 | MS.gene053520:CDS |
| TTCTTCAGCAATTTGCATAT+CGG | 0.349566 | 4.3:-26902992 | None:intergenic |
| GCAGTCACATAAAATTCCTT+TGG | 0.390666 | 4.3:-26903090 | None:intergenic |
| CACATAAAATTCCTTTGGCA+TGG | 0.535181 | 4.3:-26903085 | None:intergenic |
| GAGATTGCAACAGAGTATGC+TGG | 0.565780 | 4.3:+26902937 | MS.gene053520:CDS |
| ATATGGAATTAAGGCAGTAC+CGG | 0.575063 | 4.3:+26903014 | MS.gene053520:CDS |
| TTGCAATCTCGTCAATAACT+CGG | 0.590528 | 4.3:-26902924 | None:intergenic |
| TGGAATTAAGGCAGTACCGG+TGG | 0.616593 | 4.3:+26903017 | MS.gene053520:CDS |
| TTTATGTGACTGCAATTGAG+AGG | 0.642363 | 4.3:+26903100 | MS.gene053520:CDS |
| TGTAGTTGGTACCATGCCAA+AGG | 0.663538 | 4.3:+26903074 | MS.gene053520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTGGTTGTATTGTTTAAAAA+TGG | + | chr4.3:26903036-26903055 | MS.gene053520:CDS | 25.0% |
| !! | TTTTTAAACAATACAACCAC+CGG | - | chr4.3:26903036-26903055 | None:intergenic | 25.0% |
| AAAAAATGTGATGCTGTAGT+TGG | + | chr4.3:26903060-26903079 | MS.gene053520:CDS | 30.0% | |
| ATGCAAATTGCTGAAGAATA+TGG | + | chr4.3:26902997-26903016 | MS.gene053520:CDS | 30.0% | |
| TGCTGAAGAATATGGAATTA+AGG | + | chr4.3:26903005-26903024 | MS.gene053520:CDS | 30.0% | |
| TTCTTCAGCAATTTGCATAT+CGG | - | chr4.3:26902995-26903014 | None:intergenic | 30.0% | |
| ATATGGAATTAAGGCAGTAC+CGG | + | chr4.3:26903014-26903033 | MS.gene053520:CDS | 35.0% | |
| CACATAAAATTCCTTTGGCA+TGG | - | chr4.3:26903088-26903107 | None:intergenic | 35.0% | |
| GCAGTCACATAAAATTCCTT+TGG | - | chr4.3:26903093-26903112 | None:intergenic | 35.0% | |
| TTGCAATCTCGTCAATAACT+CGG | - | chr4.3:26902927-26902946 | None:intergenic | 35.0% | |
| TTTATGTGACTGCAATTGAG+AGG | + | chr4.3:26903100-26903119 | MS.gene053520:CDS | 35.0% | |
| GAGATTGCAACAGAGTATGC+TGG | + | chr4.3:26902937-26902956 | MS.gene053520:CDS | 45.0% | |
| ! | TGTAGTTGGTACCATGCCAA+AGG | + | chr4.3:26903074-26903093 | MS.gene053520:CDS | 45.0% |
| ! | TGGAATTAAGGCAGTACCGG+TGG | + | chr4.3:26903017-26903036 | MS.gene053520:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 26902916 | 26903137 | 26902916 | ID=MS.gene053520 |
| chr4.3 | mRNA | 26902916 | 26903137 | 26902916 | ID=MS.gene053520.t1;Parent=MS.gene053520 |
| chr4.3 | exon | 26902916 | 26903137 | 26902916 | ID=MS.gene053520.t1.exon1;Parent=MS.gene053520.t1 |
| chr4.3 | CDS | 26902916 | 26903137 | 26902916 | ID=cds.MS.gene053520.t1;Parent=MS.gene053520.t1 |
| Gene Sequence |
| Protein sequence |
