Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071772.t1 | XP_013452215.1 | 99.3 | 152 | 1 | 0 | 30 | 181 | 198 | 349 | 3.20E-78 | 301.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071772.t1 | Q9MBA2 | 95.4 | 152 | 6 | 1 | 31 | 181 | 175 | 326 | 2.6e-76 | 286.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071772.t1 | A0A072UAX9 | 99.3 | 152 | 1 | 0 | 30 | 181 | 198 | 349 | 2.3e-78 | 301.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene071772 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene071772 | 0.839244 | 1.78E-57 | -1.69E-46 |
| MS.gene049758 | MS.gene071772 | 0.817301 | 3.54E-52 | -1.69E-46 |
| MS.gene050099 | MS.gene071772 | 0.825196 | 5.35E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene071772 | 0.804815 | 1.80E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene071772 | 0.803832 | 2.89E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene071772 | -0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene071772 | 0.812502 | 4.11E-51 | -1.69E-46 |
| MS.gene050516 | MS.gene071772 | 0.813632 | 2.32E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene071772 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene050560 | MS.gene071772 | 0.810277 | 1.25E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene071772 | 0.818596 | 1.81E-52 | -1.69E-46 |
| MS.gene050787 | MS.gene071772 | 0.806597 | 7.62E-50 | -1.69E-46 |
| MS.gene051257 | MS.gene071772 | 0.821581 | 3.74E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene071772 | 0.808744 | 2.66E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene071772 | 0.810817 | 9.54E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene071772 | 0.80135 | 9.39E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene071772 | 0.814393 | 1.58E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene071772 | 0.820151 | 7.98E-53 | -1.69E-46 |
| MS.gene052256 | MS.gene071772 | 0.805708 | 1.17E-49 | -1.69E-46 |
| MS.gene052600 | MS.gene071772 | 0.83308 | 6.55E-56 | -1.69E-46 |
| MS.gene053520 | MS.gene071772 | 0.831838 | 1.33E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene071772 | 0.859135 | 5.10E-63 | -1.69E-46 |
| MS.gene054473 | MS.gene071772 | 0.859788 | 3.25E-63 | -1.69E-46 |
| MS.gene054942 | MS.gene071772 | 0.857099 | 2.06E-62 | -1.69E-46 |
| MS.gene054983 | MS.gene071772 | 0.816841 | 4.49E-52 | -1.69E-46 |
| MS.gene055303 | MS.gene071772 | 0.812207 | 4.76E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene071772 | 0.823405 | 1.41E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene071772 | 0.840252 | 9.73E-58 | -1.69E-46 |
| MS.gene057121 | MS.gene071772 | 0.858253 | 9.35E-63 | -1.69E-46 |
| MS.gene057245 | MS.gene071772 | 0.831183 | 1.93E-55 | -1.69E-46 |
| MS.gene057260 | MS.gene071772 | 0.80732 | 5.36E-50 | -1.69E-46 |
| MS.gene057439 | MS.gene071772 | 0.838052 | 3.62E-57 | -1.69E-46 |
| MS.gene057469 | MS.gene071772 | 0.808913 | 2.45E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene071772 | 0.818175 | 2.25E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene071772 | 0.833484 | 5.20E-56 | -1.69E-46 |
| MS.gene058749 | MS.gene071772 | 0.814321 | 1.64E-51 | -1.69E-46 |
| MS.gene058833 | MS.gene071772 | 0.830341 | 3.10E-55 | -1.69E-46 |
| MS.gene059518 | MS.gene071772 | 0.829793 | 4.22E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene071772 | 0.83858 | 2.64E-57 | -1.69E-46 |
| MS.gene059896 | MS.gene071772 | -0.805121 | 1.56E-49 | -1.69E-46 |
| MS.gene060165 | MS.gene071772 | 0.826223 | 3.05E-54 | -1.69E-46 |
| MS.gene060476 | MS.gene071772 | 0.808835 | 2.55E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene071772 | 0.818421 | 1.98E-52 | -1.69E-46 |
| MS.gene060994 | MS.gene071772 | 0.826995 | 2.00E-54 | -1.69E-46 |
| MS.gene061000 | MS.gene071772 | 0.824821 | 6.56E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071772.t1 | MTR_6g048300 | 96.045 | 177 | 4 | 1 | 5 | 181 | 176 | 349 | 5.61e-119 | 340 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071772.t1 | AT5G24020 | 94.805 | 154 | 7 | 1 | 29 | 181 | 173 | 326 | 6.15e-102 | 296 |
Find 0 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTAAGATGATAGTGAAT+AGG | + | 42983:2683-2702 | MS.gene071772:CDS | 20.0% |
| !!! | TATTAAGATGATAGTGAATA+GGG | + | 42983:2684-2703 | MS.gene071772:CDS | 20.0% |
| TAAAGGCGAAGATATGATGT+CGG | + | 42983:2723-2742 | MS.gene071772:CDS | 35.0% | |
| TCCCTTAATGCAGTTATATC+CGG | - | 42983:2619-2638 | None:intergenic | 35.0% | |
| TTTGAATAAGCCTCCTACTT+TGG | + | 42983:2843-2862 | MS.gene071772:CDS | 35.0% | |
| ! | AACGAGAAAAACCCTCTTTT+AGG | - | 42983:2946-2965 | None:intergenic | 35.0% |
| ! | TTATAAGGAGTACGAATCGA+GGG | + | 42983:2809-2828 | MS.gene071772:CDS | 35.0% |
| !! | AGAGGGTTTTTCTCGTTTTT+TGG | + | 42983:2949-2968 | MS.gene071772:CDS | 35.0% |
| AAAACCGGTGGAAATGGAAA+TGG | + | 42983:2445-2464 | MS.gene071772:CDS | 40.0% | |
| AGTTCCATTTCCATTTCCAC+CGG | - | 42983:2452-2471 | None:intergenic | 40.0% | |
| ATCAAAACCGCTTCATTAGC+AGG | - | 42983:2589-2608 | None:intergenic | 40.0% | |
| CAATCGATCAGGATAAAGTC+CGG | - | 42983:2526-2545 | None:intergenic | 40.0% | |
| CTTATAACCTCACTATCCTC+CGG | - | 42983:2796-2815 | None:intergenic | 40.0% | |
| GAGATAAAAACCGGTGGAAA+TGG | + | 42983:2439-2458 | MS.gene071772:CDS | 40.0% | |
| GCCGGATATAACTGCATTAA+GGG | + | 42983:2615-2634 | MS.gene071772:CDS | 40.0% | |
| GCTAGGACGGATATGATTAA+AGG | + | 42983:2706-2725 | MS.gene071772:CDS | 40.0% | |
| GGAGGCTTATTCAAAACAAG+TGG | - | 42983:2838-2857 | None:intergenic | 40.0% | |
| GGATAGTATGCAAGCTGTTA+TGG | + | 42983:2909-2928 | MS.gene071772:CDS | 40.0% | |
| TATGCAAGCTGTTATGGTTG+AGG | + | 42983:2915-2934 | MS.gene071772:CDS | 40.0% | |
| TTGAGGAAGAGCCTAAAAGA+GGG | + | 42983:2932-2951 | MS.gene071772:CDS | 40.0% | |
| TTGGATGTGCAAGAGATGTT+GGG | + | 42983:2748-2767 | MS.gene071772:CDS | 40.0% | |
| TTTGGATGTGCAAGAGATGT+TGG | + | 42983:2747-2766 | MS.gene071772:CDS | 40.0% | |
| ! | AGATGATAGTGAATAGGGCT+AGG | + | 42983:2689-2708 | MS.gene071772:CDS | 40.0% |
| ! | GTTATAAGGAGTACGAATCG+AGG | + | 42983:2808-2827 | MS.gene071772:CDS | 40.0% |
| !!! | CGAAGATATGATGTCGGTTT+TGG | + | 42983:2729-2748 | MS.gene071772:CDS | 40.0% |
| !!! | GGGTTTTTCTCGTTTTTTGG+TGG | + | 42983:2952-2971 | MS.gene071772:CDS | 40.0% |
| AAAGCCAAACCAGCCAAAGT+AGG | - | 42983:2859-2878 | None:intergenic | 45.0% | |
| AAGGGATGCAGATAGAGTGA+CGG | + | 42983:2633-2652 | MS.gene071772:CDS | 45.0% | |
| AATAAGCCTCCTACTTTGGC+TGG | + | 42983:2847-2866 | MS.gene071772:CDS | 45.0% | |
| AATGGAACTGGAACCAATGG+TGG | + | 42983:2463-2482 | MS.gene071772:CDS | 45.0% | |
| CATTACGCCTGCTAATGAAG+CGG | + | 42983:2579-2598 | MS.gene071772:CDS | 45.0% | |
| CGCCGGATATAACTGCATTA+AGG | + | 42983:2614-2633 | MS.gene071772:CDS | 45.0% | |
| CGGAGGATAGTGAGGTTATA+AGG | + | 42983:2794-2813 | MS.gene071772:CDS | 45.0% | |
| GATAGTGAATAGGGCTAGGA+CGG | + | 42983:2693-2712 | MS.gene071772:CDS | 45.0% | |
| GGAAATGGAACTGGAACCAA+TGG | + | 42983:2460-2479 | MS.gene071772:CDS | 45.0% | |
| GGTGGAAATGGAAATGGAAC+TGG | + | 42983:2451-2470 | MS.gene071772:CDS | 45.0% | |
| GTTGAGGAAGAGCCTAAAAG+AGG | + | 42983:2931-2950 | MS.gene071772:CDS | 45.0% | |
| TCCGGTATAACACCAAGCAA+CGG | - | 42983:2778-2797 | None:intergenic | 45.0% | |
| TGTTATACCGGAGGATAGTG+AGG | + | 42983:2786-2805 | MS.gene071772:CDS | 45.0% | |
| ! | AGATAGAGTGACGGGATTGT+TGG | + | 42983:2642-2661 | MS.gene071772:CDS | 45.0% |
| ! | GTTGGAGTGTGATGGGATTA+GGG | + | 42983:2660-2679 | MS.gene071772:CDS | 45.0% |
| ! | TGGCTTTTGAACAAGCTGCT+TGG | + | 42983:2872-2891 | MS.gene071772:CDS | 45.0% |
| ! | TGTTGGAGTGTGATGGGATT+AGG | + | 42983:2659-2678 | MS.gene071772:CDS | 45.0% |
| !! | AGCGGTTTTGATAACAACGC+CGG | + | 42983:2597-2616 | MS.gene071772:CDS | 45.0% |
| ACAAGCTGCTTGGAGACTTG+TGG | + | 42983:2882-2901 | MS.gene071772:CDS | 50.0% | |
| ACGGGATTGTTGGAGTGTGA+TGG | + | 42983:2652-2671 | MS.gene071772:CDS | 50.0% | |
| ACTGGAACCAATGGTGGTTC+TGG | + | 42983:2469-2488 | MS.gene071772:CDS | 50.0% | |
| AGAACCAGTGGAACTGGAGT+TGG | - | 42983:2497-2516 | None:intergenic | 50.0% | |
| AGGGATGCAGATAGAGTGAC+GGG | + | 42983:2634-2653 | MS.gene071772:CDS | 50.0% | |
| ATGTTGGGTTTGCCGTTGCT+TGG | + | 42983:2763-2782 | MS.gene071772:CDS | 50.0% | |
| CGCTTCATTAGCAGGCGTAA+TGG | - | 42983:2581-2600 | None:intergenic | 50.0% | |
| CGGGATTGTTGGAGTGTGAT+GGG | + | 42983:2653-2672 | MS.gene071772:CDS | 50.0% | |
| GTTGGAACCAGAACCACCAT+TGG | - | 42983:2479-2498 | None:intergenic | 50.0% | |
| TATCCTGATCGATTGCCCAG+CGG | + | 42983:2531-2550 | MS.gene071772:CDS | 50.0% | |
| !! | AACTCCAGTTCCACTGGTTC+TGG | + | 42983:2496-2515 | MS.gene071772:CDS | 50.0% |
| !! | GCCGTTGCTTGGTGTTATAC+CGG | + | 42983:2774-2793 | MS.gene071772:CDS | 50.0% |
| !! | GTTGCTTGGTGTTATACCGG+AGG | + | 42983:2777-2796 | MS.gene071772:CDS | 50.0% |
| !!! | TCCACCGGTTTTTATCTCCC+CGG | - | 42983:2437-2456 | None:intergenic | 50.0% |
| ACAGCCAGAACCAGTGGAAC+TGG | - | 42983:2503-2522 | None:intergenic | 55.0% | |
| ATCCTGATCGATTGCCCAGC+GGG | + | 42983:2532-2551 | MS.gene071772:CDS | 55.0% | |
| GCCAAACCAGCCAAAGTAGG+AGG | - | 42983:2856-2875 | None:intergenic | 55.0% | |
| GGTTCCAACTCCAGTTCCAC+TGG | + | 42983:2490-2509 | MS.gene071772:CDS | 55.0% | |
| TGCTTGGAGACTTGTGGAGC+AGG | + | 42983:2888-2907 | MS.gene071772:CDS | 55.0% | |
| ! | GCCTCCTACTTTGGCTGGTT+TGG | + | 42983:2852-2871 | MS.gene071772:CDS | 55.0% |
| !! | TTCCACTGGTTCTGGCTGTC+CGG | + | 42983:2504-2523 | MS.gene071772:CDS | 55.0% |
| !!! | CCACCGGTTTTTATCTCCCC+GGG | - | 42983:2436-2455 | None:intergenic | 55.0% |
| CCCGGGGAGATAAAAACCGG+TGG | + | 42983:2433-2452 | MS.gene071772:CDS | 60.0% | |
| TCCTGATCGATTGCCCAGCG+GGG | + | 42983:2533-2552 | MS.gene071772:CDS | 60.0% | |
| GTCCGGACAGCCAGAACCAG+TGG | - | 42983:2509-2528 | None:intergenic | 65.0% | |
| TCCCCGCTGGGCAATCGATC+AGG | - | 42983:2537-2556 | None:intergenic | 65.0% | |
| ! | AACCCTGCATCGATCCCCGC+TGG | - | 42983:2550-2569 | None:intergenic | 65.0% |
| ! | ACCCTGCATCGATCCCCGCT+GGG | - | 42983:2549-2568 | None:intergenic | 65.0% |
| GCCCAGCGGGGATCGATGCA+GGG | + | 42983:2545-2564 | MS.gene071772:CDS | 70.0% | |
| TGCCCAGCGGGGATCGATGC+AGG | + | 42983:2544-2563 | MS.gene071772:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42983 | gene | 2433 | 2978 | 2433 | ID=MS.gene071772 |
| 42983 | mRNA | 2433 | 2978 | 2433 | ID=MS.gene071772.t1;Parent=MS.gene071772 |
| 42983 | exon | 2433 | 2978 | 2433 | ID=MS.gene071772.t1.exon1;Parent=MS.gene071772.t1 |
| 42983 | CDS | 2433 | 2978 | 2433 | ID=cds.MS.gene071772.t1;Parent=MS.gene071772.t1 |
| Gene Sequence |
| Protein sequence |
