AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054180.t1 MTR_6g047800 96.774 279 8 1 1 278 1 279 0.0 553
MS.gene054180.t1 MTR_6g047800 96.667 270 8 1 10 278 1 270 0.0 535
MS.gene054180.t1 MTR_2g014590 77.922 231 51 0 1 231 2 232 3.81e-137 387
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054180.t1 AT5G14600 75.812 277 64 1 1 274 1 277 7.20e-160 447

Find 58 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 6.1:-28888681 MS.gene054180:CDS
TCCTGAAGAGTTTGCTGGCT+TGG 0.242918 6.1:-28887054 MS.gene054180:CDS
TAGCTCCTACTCCTGAATTA+TGG 0.261887 6.1:-28888751 MS.gene054180:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 6.1:-28888930 MS.gene054180:CDS
GGTTGTGTCGTTCTTGAATC+TGG 0.284355 6.1:-28888647 MS.gene054180:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 6.1:-28886715 MS.gene054180:CDS
GTCTAAAGTATTCAACAATA+AGG 0.308450 6.1:-28888792 MS.gene054180:CDS
TTGTTTATGTGAAAGATATA+AGG 0.311677 6.1:-28886764 MS.gene054180:intron
GATTGGATAGGAAAGTCATT+TGG 0.331486 6.1:-28888815 MS.gene054180:CDS
AAAACTAAAGTCCATAATTC+AGG 0.343342 6.1:+28888740 None:intergenic
CTTGAATCTGGCACTGGAAG+TGG 0.346516 6.1:-28888635 MS.gene054180:CDS
GCCAAGCCAGCAAACTCTTC+AGG 0.354828 6.1:+28887053 None:intergenic
ATGTATTTGGAGATTGTTCC+TGG 0.385811 6.1:-28888668 MS.gene054180:CDS
GACGGTAATGACGGCTCTAA+TGG 0.408113 6.1:-28886678 MS.gene054180:CDS
GATCCCTCAGATACTGTTAC+TGG 0.419969 6.1:+28888875 None:intergenic
ATTGGATAGGAAAGTCATTT+GGG 0.422230 6.1:-28888814 MS.gene054180:CDS
CGGGAAAGTACCGGTGAAGT+TGG 0.459909 6.1:+28886893 None:intergenic
TCTAAAGTATTCAACAATAA+GGG 0.468151 6.1:-28888791 MS.gene054180:CDS
TTGAATCTGGCACTGGAAGT+GGG 0.495210 6.1:-28888634 MS.gene054180:CDS
GTTGTACTTGTTCAATGCAC+GGG 0.495893 6.1:+28886937 None:intergenic
GTCGTTCTTGAATCTGGCAC+TGG 0.503209 6.1:-28888641 MS.gene054180:CDS
CTTTAACTACTTCACTTGCA+AGG 0.505822 6.1:-28888610 MS.gene054180:CDS
ATTTGGCAATTGTTTATGAA+AGG 0.507354 6.1:-28888913 MS.gene054180:CDS
ATGGAAAGCTCTGAGATTGA+CGG 0.512304 6.1:-28886696 MS.gene054180:CDS
TTTAAACATTCAGATTGGAT+AGG 0.513874 6.1:-28888827 MS.gene054180:CDS
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 6.1:-28888593 MS.gene054180:CDS
CGTTGTACTTGTTCAATGCA+CGG 0.540755 6.1:+28886936 None:intergenic
AAAGTCCATAATTCAGGAGT+AGG 0.545264 6.1:+28888746 None:intergenic
ATAAGCAGCTTAGTAACTGC+AGG 0.549586 6.1:-28887104 MS.gene054180:CDS
GCAGGAGTGAGGGACATTCA+AGG 0.551907 6.1:-28887086 MS.gene054180:CDS
GCTTAGTAACTGCAGGAGTG+AGG 0.562608 6.1:-28887097 MS.gene054180:CDS
GAACGTTGCCTCCTCTTGCA+AGG 0.567462 6.1:+28886648 None:intergenic
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 6.1:+28887008 None:intergenic
GTGAGGGACATTCAAGGTGA+GGG 0.573477 6.1:-28887080 MS.gene054180:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 6.1:-28886659 MS.gene054180:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 6.1:-28888609 MS.gene054180:CDS
GAAGCCAGTAACAGTATCTG+AGG 0.579372 6.1:-28888879 MS.gene054180:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 6.1:-28886903 MS.gene054180:intron
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 6.1:-28886656 MS.gene054180:CDS
ATTACAGTCACTGGATGAAG+AGG 0.584950 6.1:-28886321 MS.gene054180:intron
GATTCAAGAACGACACAACC+AGG 0.585429 6.1:+28888650 None:intergenic
AGTAACAGTATCTGAGGGAT+CGG 0.586054 6.1:-28888873 MS.gene054180:CDS
TCTGAGATTGACGGTAATGA+CGG 0.588128 6.1:-28886687 MS.gene054180:CDS
CTTAGTAACTGCAGGAGTGA+GGG 0.595594 6.1:-28887096 MS.gene054180:CDS
TTTAATAGAACAATTAGTAA+CGG 0.596180 6.1:-28888938 MS.gene054180:CDS
AAGAGGAGGCAACGTTCAGA+TGG 0.606722 6.1:-28886642 MS.gene054180:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 6.1:+28888578 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 6.1:-28886723 MS.gene054180:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 6.1:+28886727 None:intergenic
ACGTTCAGATGGAGCTAGCG+TGG 0.623569 6.1:-28886631 MS.gene054180:intron
AGTGAGGGACATTCAAGGTG+AGG 0.624772 6.1:-28887081 MS.gene054180:CDS
GGACATACGAAGTTCGAGAA+GGG 0.625372 6.1:-28886722 MS.gene054180:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 6.1:-28888544 MS.gene054180:intron
AAAGTATTCAACAATAAGGG+CGG 0.646223 6.1:-28888788 MS.gene054180:CDS
TATATAGAATCTGTGTCCTG+TGG 0.646737 6.1:+28888714 None:intergenic
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 6.1:+28887002 None:intergenic
AAGCCAGTAACAGTATCTGA+GGG 0.661239 6.1:-28888878 MS.gene054180:CDS
TTGTACTTGTTCAATGCACG+GGG 0.675813 6.1:+28886938 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTCAATTATATATAAAACA+AGG + chr6.1:28887819-28887838 None:intergenic 15.0%
!! GTAACTTATATATTGTATTA+CGG + chr6.1:28887758-28887777 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG - chr6.1:28886349-28886368 MS.gene054180:intron 15.0%
!!! TTTTTACAATGAAAAAACTT+AGG - chr6.1:28887705-28887724 MS.gene054180:intron 15.0%
!! AAATAAGATACGTATAAAAG+AGG + chr6.1:28888425-28888444 None:intergenic 20.0%
!! ACATAAACAAACATTTACAA+AGG + chr6.1:28888513-28888532 None:intergenic 20.0%
!! TAAAAATAAACTGTTGTATG+AGG + chr6.1:28888825-28888844 None:intergenic 20.0%
!! TTGTTTATGTGAAAGATATA+AGG - chr6.1:28888523-28888542 MS.gene054180:intron 20.0%
!!! TCTAAAGTATTCAACAATAA+GGG - chr6.1:28886496-28886515 MS.gene054180:intron 20.0%
! AAAACTAAAGTCCATAATTC+AGG + chr6.1:28886550-28886569 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG - chr6.1:28886800-28886819 MS.gene054180:intron 25.0%
! ATTTGGCAATTGTTTATGAA+AGG - chr6.1:28886374-28886393 MS.gene054180:intron 25.0%
! CTACCAAAAAACTAGAATAA+CGG + chr6.1:28886930-28886949 None:intergenic 25.0%
! TACGTATAAAAGAGGAAAAT+CGG + chr6.1:28888417-28888436 None:intergenic 25.0%
! TTCTAATTCTTCTTCTGTTA+TGG - chr6.1:28888686-28888705 MS.gene054180:CDS 25.0%
! TTTAAACATTCAGATTGGAT+AGG - chr6.1:28886460-28886479 MS.gene054180:intron 25.0%
! TTTCTGTAATATGCTATTGA+TGG - chr6.1:28887904-28887923 MS.gene054180:intron 25.0%
!! GTCTAAAGTATTCAACAATA+AGG - chr6.1:28886495-28886514 MS.gene054180:intron 25.0%
!! TAATTGTACTCAACTTTTGT+TGG - chr6.1:28888037-28888056 MS.gene054180:intron 25.0%
!!! GAGAATGATTTTTTCAATAG+CGG - chr6.1:28887487-28887506 MS.gene054180:intron 25.0%
!!! GTGTTTTTAAACATTCAGAT+TGG - chr6.1:28886455-28886474 MS.gene054180:intron 25.0%
AAAATCACGATCAAACTGAT+GGG + chr6.1:28887243-28887262 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG - chr6.1:28886357-28886376 MS.gene054180:intron 30.0%
ACGTATAAAAGAGGAAAATC+GGG + chr6.1:28888416-28888435 None:intergenic 30.0%
ACTGTAATGGTGTGTTTATA+TGG + chr6.1:28887025-28887044 None:intergenic 30.0%
ATTGGATAGGAAAGTCATTT+GGG - chr6.1:28886473-28886492 MS.gene054180:intron 30.0%
ATTGTAAAAATAAGCCACGT+TGG + chr6.1:28887695-28887714 None:intergenic 30.0%
CCAAACATGAATTTCCTATA+TGG + chr6.1:28888895-28888914 None:intergenic 30.0%
CCATATAGGAAATTCATGTT+TGG - chr6.1:28888892-28888911 MS.gene054180:CDS 30.0%
CTATCTTCTATTTCACATAG+CGG - chr6.1:28887879-28887898 MS.gene054180:intron 30.0%
CTTAATTCACAACAATCATG+TGG - chr6.1:28888846-28888865 MS.gene054180:CDS 30.0%
GACTTATTTCAACTGTTATG+CGG - chr6.1:28887835-28887854 MS.gene054180:intron 30.0%
GCAAAAATGTTACGATATGA+CGG - chr6.1:28888309-28888328 MS.gene054180:intron 30.0%
GCTGATATAGGAACTATATT+GGG - chr6.1:28887618-28887637 MS.gene054180:intron 30.0%
GGTATTTGTAATCTCCATAT+AGG - chr6.1:28888878-28888897 MS.gene054180:CDS 30.0%
TAATTCTGTATTACAGTCAC+TGG - chr6.1:28888957-28888976 MS.gene054180:CDS 30.0%
TATGTAATATTGCATCAGCT+AGG - chr6.1:28887406-28887425 MS.gene054180:intron 30.0%
TATTGTATTACGGTGAATAC+AGG + chr6.1:28887748-28887767 None:intergenic 30.0%
TGAAATAGAAGATAGTGTTG+CGG + chr6.1:28887874-28887893 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG + chr6.1:28887694-28887713 None:intergenic 30.0%
TTTAACTACTTCACTTGCAA+GGG - chr6.1:28886678-28886697 MS.gene054180:CDS 30.0%
TTTCTCGTTCTAGTAATTGT+AGG - chr6.1:28887954-28887973 MS.gene054180:intron 30.0%
! AAAGTATTCAACAATAAGGG+CGG - chr6.1:28886499-28886518 MS.gene054180:intron 30.0%
! AGACAAAAGAGAATTTTAGG+CGG - chr6.1:28886969-28886988 MS.gene054180:CDS 30.0%
! AGCAGACAAAAGAGAATTTT+AGG - chr6.1:28886966-28886985 MS.gene054180:CDS 30.0%
! ATACGATTTTCAGACTGTAA+TGG + chr6.1:28887038-28887057 None:intergenic 30.0%
! ATTTGATTTCCATGAGCATA+GGG - chr6.1:28887373-28887392 MS.gene054180:intron 30.0%
! CAGTCTGAAAATCGTATTTT+TGG - chr6.1:28887041-28887060 MS.gene054180:CDS 30.0%
! GAAAGATATAAGGACTTTTG+AGG - chr6.1:28888533-28888552 MS.gene054180:intron 30.0%
! TTTCTAGTGTTGTCAACTAT+TGG - chr6.1:28886841-28886860 MS.gene054180:intron 30.0%
!! ATACTTGTTTTTCTCCTTGT+AGG - chr6.1:28888139-28888158 MS.gene054180:intron 30.0%
!! ATCTTGCAATTCATTGATTC+AGG + chr6.1:28887119-28887138 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG - chr6.1:28886606-28886625 MS.gene054180:intron 30.0%
!! TACTTGTTTTTCTCCTTGTA+GGG - chr6.1:28888140-28888159 MS.gene054180:intron 30.0%
!!! AGTGTTGCAGTTTTTTTTAG+CGG + chr6.1:28886895-28886914 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG - chr6.1:28886924-28886943 MS.gene054180:CDS 30.0%
AAAGTCCATAATTCAGGAGT+AGG + chr6.1:28886544-28886563 None:intergenic 35.0%
AACGTATAAACATGTCCTGT+AGG + chr6.1:28886712-28886731 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG + chr6.1:28887447-28887466 None:intergenic 35.0%
ACCCTGCATGCAAATAAAAA+GGG + chr6.1:28887795-28887814 None:intergenic 35.0%
ACTTGAGATGTTCAATGCAA+AGG - chr6.1:28888100-28888119 MS.gene054180:intron 35.0%
ATAAACATGCCCTTCACAAA+TGG + chr6.1:28887351-28887370 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG - chr6.1:28887285-28887304 MS.gene054180:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG - chr6.1:28886619-28886638 MS.gene054180:intron 35.0%
ATTCACAACAATCATGTGGA+AGG - chr6.1:28888850-28888869 MS.gene054180:CDS 35.0%
CAAAATCACGATCAAACTGA+TGG + chr6.1:28887244-28887263 None:intergenic 35.0%
CGTAGTAGTTATGCAACTAA+TGG - chr6.1:28888460-28888479 MS.gene054180:intron 35.0%
CTTTAACTACTTCACTTGCA+AGG - chr6.1:28886677-28886696 MS.gene054180:CDS 35.0%
GAAATAGAAGATAGTGTTGC+GGG + chr6.1:28887873-28887892 None:intergenic 35.0%
GATTGGATAGGAAAGTCATT+TGG - chr6.1:28886472-28886491 MS.gene054180:intron 35.0%
GCATGCAAATAAAAAGGGAA+TGG + chr6.1:28887790-28887809 None:intergenic 35.0%
GGCTGATATAGGAACTATAT+TGG - chr6.1:28887617-28887636 MS.gene054180:intron 35.0%
GGTTATCTTCCTTGAAATTC+CGG + chr6.1:28887098-28887117 None:intergenic 35.0%
GTATGGTCGGAATCAAAATT+TGG + chr6.1:28887186-28887205 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG + chr6.1:28887097-28887116 None:intergenic 35.0%
TATATAGAATCTGTGTCCTG+TGG + chr6.1:28886576-28886595 None:intergenic 35.0%
TTCTCAAAATCATCCCTACA+AGG + chr6.1:28888156-28888175 None:intergenic 35.0%
! CATTTGATTTCCATGAGCAT+AGG - chr6.1:28887372-28887391 MS.gene054180:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG - chr6.1:28886733-28886752 MS.gene054180:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG - chr6.1:28886433-28886452 MS.gene054180:intron 35.0%
!! AGGGATGATTTTGAGAAGAT+TGG - chr6.1:28888159-28888178 MS.gene054180:intron 35.0%
!! TCCCTTTTTATTTGCATGCA+GGG - chr6.1:28887791-28887810 MS.gene054180:intron 35.0%
!! TTCCCTTTTTATTTGCATGC+AGG - chr6.1:28887790-28887809 MS.gene054180:intron 35.0%
!!! ATCGTATTTTTGGTGAAACG+TGG - chr6.1:28887051-28887070 MS.gene054180:CDS 35.0%
AACAAAGATCTGGATCAGCT+AGG - chr6.1:28886743-28886762 MS.gene054180:CDS 40.0%
AAGCCAGTAACAGTATCTGA+GGG - chr6.1:28886409-28886428 MS.gene054180:intron 40.0%
ACAATCATGTGGAAGGTTTC+TGG - chr6.1:28888857-28888876 MS.gene054180:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG - chr6.1:28888384-28888403 MS.gene054180:intron 40.0%
ACCGCAGAAAATTGTAGGTA+CGG + chr6.1:28887146-28887165 None:intergenic 40.0%
ACTATAGTGCTATCACATGG+TGG - chr6.1:28887511-28887530 MS.gene054180:intron 40.0%
AGTAACAGTATCTGAGGGAT+CGG - chr6.1:28886414-28886433 MS.gene054180:intron 40.0%
ATAAGCAGCTTAGTAACTGC+AGG - chr6.1:28888183-28888202 MS.gene054180:intron 40.0%
ATCAAACATCAGAGGAAGTC+AGG + chr6.1:28887439-28887458 None:intergenic 40.0%
ATTACAGTCACTGGATGAAG+AGG - chr6.1:28888966-28888985 MS.gene054180:CDS 40.0%
CACCCTGCATGCAAATAAAA+AGG + chr6.1:28887796-28887815 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG - chr6.1:28887288-28887307 MS.gene054180:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG + chr6.1:28887290-28887309 None:intergenic 40.0%
CGTTGTACTTGTTCAATGCA+CGG + chr6.1:28888354-28888373 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG - chr6.1:28887289-28887308 MS.gene054180:intron 40.0%
GGTCGGAATCAAAATTTGGT+GGG + chr6.1:28887182-28887201 None:intergenic 40.0%
GTTGTACTTGTTCAATGCAC+GGG + chr6.1:28888353-28888372 None:intergenic 40.0%
TAGCTCCTACTCCTGAATTA+TGG - chr6.1:28886536-28886555 MS.gene054180:intron 40.0%
TGGTCGGAATCAAAATTTGG+TGG + chr6.1:28887183-28887202 None:intergenic 40.0%
TTGTACTTGTTCAATGCACG+GGG + chr6.1:28888352-28888371 None:intergenic 40.0%
! ACCGTACCTACAATTTTCTG+CGG - chr6.1:28887142-28887161 MS.gene054180:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG + chr6.1:28888303-28888322 None:intergenic 40.0%
! TCTGAGATTGACGGTAATGA+CGG - chr6.1:28888600-28888619 MS.gene054180:CDS 40.0%
! TGCTATTGATGGCACTTTCA+TGG - chr6.1:28887915-28887934 MS.gene054180:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG - chr6.1:28888268-28888287 MS.gene054180:intron 40.0%
!! ATGGAAAGCTCTGAGATTGA+CGG - chr6.1:28888591-28888610 MS.gene054180:CDS 40.0%
!! CGTATGATTTTGATACCCCT+TGG + chr6.1:28887218-28887237 None:intergenic 40.0%
!! CTATTGGCTCTATAGCATAG+CGG - chr6.1:28886857-28886876 MS.gene054180:intron 40.0%
!! GAAGTTTTGCTGGCTGATAT+AGG - chr6.1:28887606-28887625 MS.gene054180:intron 40.0%
!! TTTTTGGTGAAACGTGGTCA+TGG - chr6.1:28887057-28887076 MS.gene054180:CDS 40.0%
!!! ATTTTAGGCGGTGTTGTCAA+TGG - chr6.1:28886981-28887000 MS.gene054180:CDS 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG - chr6.1:28888251-28888270 MS.gene054180:intron 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG - chr6.1:28887251-28887270 MS.gene054180:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG - chr6.1:28886557-28886576 MS.gene054180:intron 40.0%
AACATGCCCTTCACAAATGG+TGG + chr6.1:28887348-28887367 None:intergenic 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG - chr6.1:28888628-28888647 MS.gene054180:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG + chr6.1:28887291-28887310 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG - chr6.1:28888572-28888591 MS.gene054180:CDS 45.0%
CTTAGTAACTGCAGGAGTGA+GGG - chr6.1:28888191-28888210 MS.gene054180:intron 45.0%
GAAGCCAGTAACAGTATCTG+AGG - chr6.1:28886408-28886427 MS.gene054180:intron 45.0%
GATCCCTCAGATACTGTTAC+TGG + chr6.1:28886415-28886434 None:intergenic 45.0%
GATTCAAGAACGACACAACC+AGG + chr6.1:28886640-28886659 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+TGG + chr6.1:28887143-28887162 None:intergenic 45.0%
GGACATACGAAGTTCGAGAA+GGG - chr6.1:28888565-28888584 MS.gene054180:CDS 45.0%
GGATCACCGCAGAAAATTGT+AGG + chr6.1:28887151-28887170 None:intergenic 45.0%
GGCACTATAGTGCTATCACA+TGG - chr6.1:28887508-28887527 MS.gene054180:intron 45.0%
TCCTTGAAATTCCGGGTTCA+CGG + chr6.1:28887090-28887109 None:intergenic 45.0%
TCTGTTATGGCTAGACCATG+TGG - chr6.1:28888699-28888718 MS.gene054180:CDS 45.0%
TGAGTCCACTATGAAAACCG+TGG - chr6.1:28887541-28887560 MS.gene054180:intron 45.0%
TGCTTGTATACACGTGTGTG+CGG - chr6.1:28888071-28888090 MS.gene054180:intron 45.0%
TTTCGCTATCAGGACAGTAG+CGG - chr6.1:28887575-28887594 MS.gene054180:intron 45.0%
! ACAATTTTCTGCGGTGATCC+TGG - chr6.1:28887151-28887170 MS.gene054180:intron 45.0%
! ACTGTCCTGATAGCGAAACT+GGG + chr6.1:28887573-28887592 None:intergenic 45.0%
! GAGGAAAATCGGGAAAGTAC+CGG + chr6.1:28888406-28888425 None:intergenic 45.0%
! GGCTGCTATTGAAGTTTTGC+TGG - chr6.1:28887596-28887615 MS.gene054180:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG - chr6.1:28886640-28886659 MS.gene054180:CDS 45.0%
! GGTTTTCCTGAAGAGTTTGC+TGG - chr6.1:28888228-28888247 MS.gene054180:intron 45.0%
! TACTGTCCTGATAGCGAAAC+TGG + chr6.1:28887574-28887593 None:intergenic 45.0%
! TTGAATCTGGCACTGGAAGT+GGG - chr6.1:28886653-28886672 MS.gene054180:CDS 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG - chr6.1:28887257-28887276 MS.gene054180:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG - chr6.1:28888544-28888563 MS.gene054180:intron 45.0%
AAATTCCGGGTTCACGGGTT+GGG + chr6.1:28887084-28887103 None:intergenic 50.0%
AAGAGGAGGCAACGTTCAGA+TGG - chr6.1:28888645-28888664 MS.gene054180:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG - chr6.1:28887199-28887218 MS.gene054180:intron 50.0%
AGACCATGTGGTGAAGCTAG+AGG - chr6.1:28888711-28888730 MS.gene054180:CDS 50.0%
AGTGAGGGACATTCAAGGTG+AGG - chr6.1:28888206-28888225 MS.gene054180:intron 50.0%
CCTTGAAATTCCGGGTTCAC+GGG + chr6.1:28887089-28887108 None:intergenic 50.0%
CGGACATACGAAGTTCGAGA+AGG - chr6.1:28888564-28888583 MS.gene054180:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG + chr6.1:28888563-28888582 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG - chr6.1:28887377-28887396 MS.gene054180:intron 50.0%
GCGGGCATCCCTTCATAAAT+TGG - chr6.1:28887279-28887298 MS.gene054180:intron 50.0%
GCTATCCCAGTTTCGCTATC+AGG - chr6.1:28887565-28887584 MS.gene054180:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG + chr6.1:28888288-28888307 None:intergenic 50.0%
GCTTAGTAACTGCAGGAGTG+AGG - chr6.1:28888190-28888209 MS.gene054180:intron 50.0%
GGAATAGCTAGCCAAGGTTG+AGG + chr6.1:28888282-28888301 None:intergenic 50.0%
GTCGTTCTTGAATCTGGCAC+TGG - chr6.1:28886646-28886665 MS.gene054180:CDS 50.0%
GTGAGGGACATTCAAGGTGA+GGG - chr6.1:28888207-28888226 MS.gene054180:intron 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG - chr6.1:28888631-28888650 MS.gene054180:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG + chr6.1:28887199-28887218 None:intergenic 50.0%
TTTGATACCCCTTGGCATGC+TGG + chr6.1:28887210-28887229 None:intergenic 50.0%
! AAAATTTGGTGGGTGCAGCC+AGG + chr6.1:28887172-28887191 None:intergenic 50.0%
! CTTGAATCTGGCACTGGAAG+TGG - chr6.1:28886652-28886671 MS.gene054180:CDS 50.0%
! GACGGTAATGACGGCTCTAA+TGG - chr6.1:28888609-28888628 MS.gene054180:CDS 50.0%
! TAGCGCCACGGTTTTCATAG+TGG + chr6.1:28887549-28887568 None:intergenic 50.0%
! TGACCTCTAGCTTCACCACA+TGG + chr6.1:28888717-28888736 None:intergenic 50.0%
! TTTGACTTCCTTGGCCATGG+CGG - chr6.1:28887260-28887279 MS.gene054180:intron 50.0%
!! TCCTGAAGAGTTTGCTGGCT+TGG - chr6.1:28888233-28888252 MS.gene054180:intron 50.0%
!! TTAGGCGGTGTTGTCAATGG+TGG - chr6.1:28886984-28887003 MS.gene054180:CDS 50.0%
ACCGTGGCCACCATTTGTGA+AGG - chr6.1:28887338-28887357 MS.gene054180:intron 55.0%
ACGTTCAGATGGAGCTAGCG+TGG - chr6.1:28888656-28888675 MS.gene054180:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG - chr6.1:28887678-28887697 MS.gene054180:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG - chr6.1:28887200-28887219 MS.gene054180:intron 55.0%
CCCGTGAACCCGGAATTTCA+AGG - chr6.1:28887086-28887105 MS.gene054180:CDS 55.0%
CCCTTCACAAATGGTGGCCA+CGG + chr6.1:28887342-28887361 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG - chr6.1:28887339-28887358 MS.gene054180:intron 55.0%
CTGATCCAGCCCTATGCTCA+TGG + chr6.1:28887385-28887404 None:intergenic 55.0%
GAAATTCCGGGTTCACGGGT+TGG + chr6.1:28887085-28887104 None:intergenic 55.0%
GAACGTTGCCTCCTCTTGCA+AGG + chr6.1:28888642-28888661 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG - chr6.1:28887198-28887217 MS.gene054180:intron 55.0%
GCAGGAGTGAGGGACATTCA+AGG - chr6.1:28888201-28888220 MS.gene054180:intron 55.0%
GCCAAGCCAGCAAACTCTTC+AGG + chr6.1:28888237-28888256 None:intergenic 55.0%
GGTGAAGCTAGAGGTCACAC+TGG - chr6.1:28888720-28888739 MS.gene054180:CDS 55.0%
TCTGCCTCACAATTCCACCG+TGG - chr6.1:28887322-28887341 MS.gene054180:intron 55.0%
TTATGAAGGGATGCCCGCCA+TGG + chr6.1:28887277-28887296 None:intergenic 55.0%
TTCACAAATGGTGGCCACGG+TGG + chr6.1:28887339-28887358 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+GGG - chr6.1:28887261-28887280 MS.gene054180:intron 55.0%
!! CGGGAAAGTACCGGTGAAGT+TGG + chr6.1:28888397-28888416 None:intergenic 55.0%
!! GCGGTGTTGTCAATGGTGGA+AGG - chr6.1:28886988-28887007 MS.gene054180:CDS 55.0%
ATGGACCCAACCCGTGAACC+CGG - chr6.1:28887076-28887095 MS.gene054180:CDS 60.0%
CCCCTTGGCATGCTGGAGTA+TGG + chr6.1:28887203-28887222 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG - chr6.1:28886694-28886713 MS.gene054180:CDS 60.0%
GCGAAACTGGGATAGCGCCA+CGG + chr6.1:28887561-28887580 None:intergenic 60.0%
GTGGCCACGGTGGAATTGTG+AGG + chr6.1:28887329-28887348 None:intergenic 60.0%
AGGGATGCCCGCCATGGCCA+AGG + chr6.1:28887271-28887290 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 28886316 28888993 28886316 ID=MS.gene054180
chr6.1 mRNA 28886316 28888993 28886316 ID=MS.gene054180.t1;Parent=MS.gene054180
chr6.1 exon 28888545 28888993 28888545 ID=MS.gene054180.t1.exon1;Parent=MS.gene054180.t1
chr6.1 CDS 28888545 28888993 28888545 ID=cds.MS.gene054180.t1;Parent=MS.gene054180.t1
chr6.1 exon 28886904 28887148 28886904 ID=MS.gene054180.t1.exon2;Parent=MS.gene054180.t1
chr6.1 CDS 28886904 28887148 28886904 ID=cds.MS.gene054180.t1;Parent=MS.gene054180.t1
chr6.1 exon 28886632 28886771 28886632 ID=MS.gene054180.t1.exon3;Parent=MS.gene054180.t1
chr6.1 CDS 28886632 28886771 28886632 ID=cds.MS.gene054180.t1;Parent=MS.gene054180.t1
chr6.1 exon 28886316 28886336 28886316 ID=MS.gene054180.t1.exon4;Parent=MS.gene054180.t1
chr6.1 CDS 28886316 28886336 28886316 ID=cds.MS.gene054180.t1;Parent=MS.gene054180.t1
Gene Sequence

>MS.gene054180

ATGTTGGCTATCGATTCTACAAGAAAGATATCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGAAAGGCATGATAGCATGAAGCCAGTAACAGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTTAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAACAATAAGGGCGGTTTTGTTTACTTATTAGCTCCTACTCCTGAATTATGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATATTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGCACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTGTCTTTCTCTGACGATATTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAGTGTTGTCAACTATTGGCTCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGACAAAAGAGAATTTTAGGCGGTGTTGTCAATGGTGGAAGGCTAAAAATCTGCCATATAAACACACCATTACAGTCTGAAAATCGTATTTTTGGTGAAACGTGGTCATGGACCCAACCCGTGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCACCGTACCTACAATTTTCTGCGGTGATCCTGGCTGCACCCACCAAATTTTGATTCCGACCATACTCCAGCATGCCAAGGGGTATCAAAATCATACGATCCCATCAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCGGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCATTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATCAGCTATGTAATATTGCATCAGCTAGGTGCTGCACCTGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGAATGATTTTTTCAATAGCGGCACTATAGTGCTATCACATGGTGGATTTTGATGAGTCCACTATGAAAACCGTGGCGCTATCCCAGTTTCGCTATCAGGACAGTAGCGGCTGCTATTGAAGTTTTGCTGGCTGATATAGGAACTATATTGGGACTTTTTTTCATTTTTTTTAAAATAATAAAATTGAAGATCTGCATGTGTGCCCAACGTGGCTTATTTTTACAATGAAAAAACTTAGGAGTCAGTGAATATTACTCCTGTATTCACCGTAATACAATATATAAGTTACGTTCTGCTTCCATTCCCTTTTTATTTGCATGCAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTGCCCGCAACACTATCTTCTATTTCACATAGCGGATTTTCTGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTTTTTTCTCGTTCTAGTAATTGTAGGTAATATTTTATGTGATCTAATACTACTTTCAGAATGTCAGTGCTTAAGTGCAAATTTATTTAATTGTACTCAACTTTTGTTGGATTCATTTATGTGCTTGTATACACGTGTGTGCGGTGATAAACTTGAGATGTTCAATGCAAAGGTGAATTAGAAATATGAATACTTGTTTTTCTCCTTGTAGGGATGATTTTGAGAAGATTGGCATAAGCAGCTTAGTAACTGCAGGAGTGAGGGACATTCAAGGTGAGGGTTTTCCTGAAGAGTTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGATATGACGGCACTTTATGTTCTTTCTCCCCGTGCATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGGTACTTTCCCGATTTTCCTCTTTTATACGTATCTTATTTTTGCTCTCTCTCACACGTAGTAGTTATGCAACTAATGGTAATTTTATTCAAGCACTGACAAAACTCCTTTGTAAATGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTCTGAGATTGACGGTAATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCAGATGGAGCTAGCGTGGTGAGCTATTCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAAATGTCTGTCATCAAGATTATTATTGTAATTTTATGATCTCTTCAATTTTGACCTCATACAACAGTTTATTTTTATCTTAATTCACAACAATCATGTGGAAGGTTTCTGGTATTTGTAATCTCCATATAGGAAATTCATGTTTGGACAATGTCTTTTTTTTGTTTGTTTTATAATTAAAATATTTGATAATTCTGTATTACAGTCACTGGATGAAGAGGTTTGA
Protein sequence

>MS.gene054180.t1

MLAIDSTRKISFNRTISNGDLAIVYERHDSMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFNNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRYDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESSEIDGNDGSNGSLPCKRRQRSDGASVSLDEEV