AlfalfaGEDB | MS.gene05524

M. sativa cultivar XinJiangDaYe / MS.gene05524

KEGG: K03627
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01381.25 PF08523.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05524.t1	AES76734.2	97.3	146	4	0	1	146	4	149	2.60E-70	274.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05524.t1	Q9LV58	71.6	148	40	1	1	146	1	148	8.2e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05524.t1	G7KNH9	97.3	146	4	0	1	146	4	149	1.9e-70	274.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene05524.t1	TR	MBF1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05524	MS.gene51432	0.991445	1.09E-187	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene05524	MS.gene91241	PPI
MS.gene045483	MS.gene05524	PPI
MS.gene05524	MS.gene006697	PPI
MS.gene05524	MS.gene073154	PPI
MS.gene05524	MS.gene73865	PPI
MS.gene05524	MS.gene35285	PPI
MS.gene05524	MS.gene045483	PPI
MS.gene91241	MS.gene05524	PPI
MS.gene05524	MS.gene94585	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05524.t1	MTR_6g086280	97.260	146	4	0	1	146	4	149	1.78e-100	285
MS.gene05524.t1	MTR_6g086280	97.222	144	4	0	1	144	4	147	4.12e-99	281
MS.gene05524.t1	MTR_2g084220	50.000	138	69	0	6	143	2	139	4.64e-43	139
MS.gene05524.t1	MTR_4g080090	50.725	138	68	0	6	143	4	141	9.39e-42	136
MS.gene05524.t1	MTR_6g018330	42.105	133	61	1	11	143	26	142	1.70e-26	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05524.t1	AT3G24500	71.622	148	40	1	1	146	1	148	9.93e-69	204
MS.gene05524.t1	AT3G58680	52.174	138	66	0	6	143	4	141	4.19e-44	142
MS.gene05524.t1	AT2G42680	50.725	138	68	0	6	143	4	141	7.40e-43	139
MS.gene05524.t1	AT3G24500	58.947	95	37	1	1	93	1	95	8.16e-33	112

Find 43 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGGAAAATGGAGAAGGTTT+TGG	0.237217	6.2:+84572985	MS.gene05524:CDS
TGTTATTAATCAGGGTGTTT+TGG	0.262576	6.2:+84572964	MS.gene05524:CDS
GTTAAACTTAGGGGTAAAAT+TGG	0.277746	6.2:+84573010	MS.gene05524:CDS
AGGGCTTGGTTTATTGCCTT+TGG	0.277912	6.2:-84572689	None:intergenic
CTGCTCCGGTTCGGAGGGCT+TGG	0.299889	6.2:-84572703	None:intergenic
TTACGAAGGTCTTGAGCTTT+GGG	0.313356	6.2:-84572665	None:intergenic
TGAAAGGGTTCAGGTTGTTC+AGG	0.318932	6.2:+84572922	MS.gene05524:CDS
CGCGATACAGAAAGCGCGTT+TGG	0.332684	6.2:+84572859	MS.gene05524:CDS
GTTACGAAGGTCTTGAGCTT+TGG	0.336478	6.2:-84572666	None:intergenic
ATTGATTAATGAAAGGGTTC+AGG	0.341931	6.2:+84572913	MS.gene05524:CDS
TGGTAAAGCTGTTATTAATC+AGG	0.346445	6.2:+84572955	MS.gene05524:CDS
GAAGATGAGTCAGGGTGATT+TGG	0.359012	6.2:+84572886	MS.gene05524:CDS
GGGCTTGGTTTATTGCCTTT+GGG	0.364374	6.2:-84572688	None:intergenic
AGCTTCCTCGCGTTTAAAAC+CGG	0.385062	6.2:-84572782	None:intergenic
GGCGAAATTGATTAATGAAA+GGG	0.407053	6.2:+84572907	MS.gene05524:CDS
CTGAACCAGCAGCGTTGGAG+CGG	0.424927	6.2:+84572816	MS.gene05524:CDS
AAGGTCTTGAGCTTTGGGTT+TGG	0.439281	6.2:-84572660	None:intergenic
TAGGAACAATAAAACAAGAC+TGG	0.459948	6.2:+84572615	MS.gene05524:CDS
GCAGCGTTGGAGCGGGTTGG+TGG	0.474323	6.2:+84572824	MS.gene05524:CDS
TGGCGAAATTGATTAATGAA+AGG	0.476549	6.2:+84572906	MS.gene05524:CDS
TTTGGATAAGAAGATGAGTC+AGG	0.478923	6.2:+84572877	MS.gene05524:CDS
ATAAACCAAGCCCTCCGAAC+CGG	0.491593	6.2:+84572698	MS.gene05524:CDS
AGCGGCTGAACCAGCAGCGT+TGG	0.495442	6.2:+84572811	MS.gene05524:CDS
GACTGTTAAGACCTCTGCTC+CGG	0.499262	6.2:-84572717	None:intergenic
TTTAAAACCGGTCCGCTCGT+TGG	0.502945	6.2:-84572770	None:intergenic
TTAAGACCTCTGCTCCGGTT+CGG	0.505245	6.2:-84572712	None:intergenic
CCAACCCGCTCCAACGCTGC+TGG	0.509376	6.2:-84572821	None:intergenic
GACCTCTGCTCCGGTTCGGA+GGG	0.511130	6.2:-84572708	None:intergenic
TGAACCAGCAGCGTTGGAGC+GGG	0.514144	6.2:+84572817	MS.gene05524:CDS
AGACCTCTGCTCCGGTTCGG+AGG	0.519624	6.2:-84572709	None:intergenic
ATTGTTCCTACTGTTCGTGT+CGG	0.524804	6.2:-84572602	None:intergenic
GGTAAAGCTGTTATTAATCA+GGG	0.553453	6.2:+84572956	MS.gene05524:CDS
CGCGAGGAAGCTAGATGAAG+CGG	0.564479	6.2:+84572793	MS.gene05524:CDS
GTTGTTCAGGAGTATGAGAA+TGG	0.565499	6.2:+84572935	MS.gene05524:CDS
AGCCCTCCGAACCGGAGCAG+AGG	0.582656	6.2:+84572706	MS.gene05524:CDS
CCAGCAGCGTTGGAGCGGGT+TGG	0.583304	6.2:+84572821	MS.gene05524:CDS
ACGATGCCGACACGAACAGT+AGG	0.590972	6.2:+84572596	None:intergenic
TTATTGCCTTTGGGTTACGA+AGG	0.607239	6.2:-84572679	None:intergenic
AGGAACAATAAAACAAGACT+GGG	0.609753	6.2:+84572616	MS.gene05524:CDS
AAAACAAGACTGGGAACCCG+TGG	0.638139	6.2:+84572625	MS.gene05524:CDS
ACAGTCAAGAAACCAACAGC+CGG	0.641212	6.2:+84572734	MS.gene05524:CDS
TTGGATAAGAAGATGAGTCA+GGG	0.654305	6.2:+84572878	MS.gene05524:CDS
TCAAGACCTTCGTAACCCAA+AGG	0.668433	6.2:+84572673	MS.gene05524:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGAACAATAAAACAAGACT+GGG	+	chr6.2:84572616-84572635	MS.gene05524:CDS	30.0%
	ATTGATTAATGAAAGGGTTC+AGG	+	chr6.2:84572913-84572932	MS.gene05524:CDS	30.0%
	GGCGAAATTGATTAATGAAA+GGG	+	chr6.2:84572907-84572926	MS.gene05524:CDS	30.0%
	GGTAAAGCTGTTATTAATCA+GGG	+	chr6.2:84572956-84572975	MS.gene05524:CDS	30.0%
	GTTAAACTTAGGGGTAAAAT+TGG	+	chr6.2:84573010-84573029	MS.gene05524:CDS	30.0%
	TAGGAACAATAAAACAAGAC+TGG	+	chr6.2:84572615-84572634	MS.gene05524:CDS	30.0%
	TGGCGAAATTGATTAATGAA+AGG	+	chr6.2:84572906-84572925	MS.gene05524:CDS	30.0%
	TGGTAAAGCTGTTATTAATC+AGG	+	chr6.2:84572955-84572974	MS.gene05524:CDS	30.0%
!!	GTTATTAATCAGGGTGTTTT+GGG	+	chr6.2:84572965-84572984	MS.gene05524:CDS	30.0%
!!	TGTTATTAATCAGGGTGTTT+TGG	+	chr6.2:84572964-84572983	MS.gene05524:CDS	30.0%
!!	TTATTAATCAGGGTGTTTTG+GGG	+	chr6.2:84572966-84572985	MS.gene05524:CDS	30.0%
	TTGGATAAGAAGATGAGTCA+GGG	+	chr6.2:84572878-84572897	MS.gene05524:CDS	35.0%
	TTTGGATAAGAAGATGAGTC+AGG	+	chr6.2:84572877-84572896	MS.gene05524:CDS	35.0%
	ATTGTTCCTACTGTTCGTGT+CGG	-	chr6.2:84572605-84572624	None:intergenic	40.0%
	TCGAACAAAAAACCAACGAG+CGG	+	chr6.2:84572758-84572777	MS.gene05524:CDS	40.0%
	TTACGAAGGTCTTGAGCTTT+GGG	-	chr6.2:84572668-84572687	None:intergenic	40.0%
!	GGAAAATGGAGAAGGTTTTG+GGG	+	chr6.2:84572987-84573006	MS.gene05524:CDS	40.0%
!	GGGAAAATGGAGAAGGTTTT+GGG	+	chr6.2:84572986-84573005	MS.gene05524:CDS	40.0%
!	GTTGTTCAGGAGTATGAGAA+TGG	+	chr6.2:84572935-84572954	MS.gene05524:CDS	40.0%
!	TTATTGCCTTTGGGTTACGA+AGG	-	chr6.2:84572682-84572701	None:intergenic	40.0%
!!	GAAAATGGAGAAGGTTTTGG+GGG	+	chr6.2:84572988-84573007	MS.gene05524:CDS	40.0%
!!!	AGGTTTTGGGGGTTAAACTT+AGG	+	chr6.2:84572999-84573018	MS.gene05524:CDS	40.0%
!!!	CGTTGGTTTTTTGTTCGAAC+CGG	-	chr6.2:84572756-84572775	None:intergenic	40.0%
!!!	GGTTTTGGGGGTTAAACTTA+GGG	+	chr6.2:84573000-84573019	MS.gene05524:CDS	40.0%
!!!	GTTTTGGGGGTTAAACTTAG+GGG	+	chr6.2:84573001-84573020	MS.gene05524:CDS	40.0%
!!!	TGTTTTGGGGAAAATGGAGA+AGG	+	chr6.2:84572979-84572998	MS.gene05524:CDS	40.0%
!!!	TTTGGTTTTGTGGAGAACCA+CGG	-	chr6.2:84572645-84572664	None:intergenic	40.0%
	ACAGTCAAGAAACCAACAGC+CGG	+	chr6.2:84572734-84572753	MS.gene05524:CDS	45.0%
	GAAGATGAGTCAGGGTGATT+TGG	+	chr6.2:84572886-84572905	MS.gene05524:CDS	45.0%
	GTTACGAAGGTCTTGAGCTT+TGG	-	chr6.2:84572669-84572688	None:intergenic	45.0%
	TCAAGACCTTCGTAACCCAA+AGG	+	chr6.2:84572673-84572692	MS.gene05524:CDS	45.0%
!	AGCTTCCTCGCGTTTAAAAC+CGG	-	chr6.2:84572785-84572804	None:intergenic	45.0%
!	AGGGCTTGGTTTATTGCCTT+TGG	-	chr6.2:84572692-84572711	None:intergenic	45.0%
!	GGGCTTGGTTTATTGCCTTT+GGG	-	chr6.2:84572691-84572710	None:intergenic	45.0%
!	GGGGAAAATGGAGAAGGTTT+TGG	+	chr6.2:84572985-84573004	MS.gene05524:CDS	45.0%
!	TTTTTGTTCGAACCGGCTGT+TGG	-	chr6.2:84572749-84572768	None:intergenic	45.0%
!!	AAGGTCTTGAGCTTTGGGTT+TGG	-	chr6.2:84572663-84572682	None:intergenic	45.0%
!!	TGAAAGGGTTCAGGTTGTTC+AGG	+	chr6.2:84572922-84572941	MS.gene05524:CDS	45.0%
!!!	GAGCTTTGGGTTTGGTTTTG+TGG	-	chr6.2:84572655-84572674	None:intergenic	45.0%
!!!	TCAGGGTGTTTTGGGGAAAA+TGG	+	chr6.2:84572973-84572992	MS.gene05524:CDS	45.0%
!!!	TTGGTTTTGTGGAGAACCAC+GGG	-	chr6.2:84572644-84572663	None:intergenic	45.0%
	AAAACAAGACTGGGAACCCG+TGG	+	chr6.2:84572625-84572644	MS.gene05524:CDS	50.0%
	ATAAACCAAGCCCTCCGAAC+CGG	+	chr6.2:84572698-84572717	MS.gene05524:CDS	50.0%
	CAAAAAACCAACGAGCGGAC+CGG	+	chr6.2:84572763-84572782	MS.gene05524:CDS	50.0%
	GACTGTTAAGACCTCTGCTC+CGG	-	chr6.2:84572720-84572739	None:intergenic	50.0%
	TTAAGACCTCTGCTCCGGTT+CGG	-	chr6.2:84572715-84572734	None:intergenic	50.0%
!	TTTAAAACCGGTCCGCTCGT+TGG	-	chr6.2:84572773-84572792	None:intergenic	50.0%
	CGCGAGGAAGCTAGATGAAG+CGG	+	chr6.2:84572793-84572812	MS.gene05524:CDS	55.0%
!	CGCGATACAGAAAGCGCGTT+TGG	+	chr6.2:84572859-84572878	MS.gene05524:CDS	55.0%
!	CTGAACCAGCAGCGTTGGAG+CGG	+	chr6.2:84572816-84572835	MS.gene05524:CDS	60.0%
!	TGAACCAGCAGCGTTGGAGC+GGG	+	chr6.2:84572817-84572836	MS.gene05524:CDS	60.0%
!!!	GCGGACCGGTTTTAAACGCG+AGG	+	chr6.2:84572777-84572796	MS.gene05524:CDS	60.0%
	AGCGGCTGAACCAGCAGCGT+TGG	+	chr6.2:84572811-84572830	MS.gene05524:CDS	65.0%
!!	AGACCTCTGCTCCGGTTCGG+AGG	-	chr6.2:84572712-84572731	None:intergenic	65.0%
!!	GACCTCTGCTCCGGTTCGGA+GGG	-	chr6.2:84572711-84572730	None:intergenic	65.0%
	AGCCCTCCGAACCGGAGCAG+AGG	+	chr6.2:84572706-84572725	MS.gene05524:CDS	70.0%
	CCAACCCGCTCCAACGCTGC+TGG	-	chr6.2:84572824-84572843	None:intergenic	70.0%
!	CCAGCAGCGTTGGAGCGGGT+TGG	+	chr6.2:84572821-84572840	MS.gene05524:CDS	70.0%
!!	CTGCTCCGGTTCGGAGGGCT+TGG	-	chr6.2:84572706-84572725	None:intergenic	70.0%
!!	GCAGCGTTGGAGCGGGTTGG+TGG	+	chr6.2:84572824-84572843	MS.gene05524:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	84572599	84573039	84572599	ID=MS.gene05524
chr6.2	mRNA	84572599	84573039	84572599	ID=MS.gene05524.t1;Parent=MS.gene05524
chr6.2	exon	84572599	84573039	84572599	ID=MS.gene05524.t1.exon1;Parent=MS.gene05524.t1
chr6.2	CDS	84572599	84573039	84572599	ID=cds.MS.gene05524.t1;Parent=MS.gene05524.t1

Gene Sequence

>MS.gene05524

ATGCCGACACGAACAGTAGGAACAATAAAACAAGACTGGGAACCCGTGGTTCTCCACAAAACCAAACCCAAAGCTCAAGACCTTCGTAACCCAAAGGCAATAAACCAAGCCCTCCGAACCGGAGCAGAGGTCTTAACAGTCAAGAAACCAACAGCCGGTTCGAACAAAAAACCAACGAGCGGACCGGTTTTAAACGCGAGGAAGCTAGATGAAGCGGCTGAACCAGCAGCGTTGGAGCGGGTTGGTGGAGAAGTGAGACACGCGATACAGAAAGCGCGTTTGGATAAGAAGATGAGTCAGGGTGATTTGGCGAAATTGATTAATGAAAGGGTTCAGGTTGTTCAGGAGTATGAGAATGGTAAAGCTGTTATTAATCAGGGTGTTTTGGGGAAAATGGAGAAGGTTTTGGGGGTTAAACTTAGGGGTAAAATTGGAAAATAA

Protein sequence

>MS.gene05524.t1

MPTRTVGTIKQDWEPVVLHKTKPKAQDLRNPKAINQALRTGAEVLTVKKPTAGSNKKPTSGPVLNARKLDEAAEPAALERVGGEVRHAIQKARLDKKMSQGDLAKLINERVQVVQEYENGKAVINQGVLGKMEKVLGVKLRGKIGK