AlfalfaGEDB | MS.gene51432

M. sativa cultivar XinJiangDaYe / MS.gene51432

KEGG: K03627
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01381.25 PF08523.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51432.t1	AES76734.2	97.3	146	4	0	1	146	4	149	2.60E-70	274.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51432.t1	Q9LV58	71.6	148	40	1	1	146	1	148	8.2e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51432.t1	G7KNH9	97.3	146	4	0	1	146	4	149	1.9e-70	274.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene51432.t1	TR	MBF1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05524	MS.gene51432	0.991445	1.09E-187	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene91241	MS.gene51432	PPI
MS.gene94585	MS.gene51432	PPI
MS.gene045483	MS.gene51432	PPI
MS.gene006697	MS.gene51432	PPI
MS.gene51432	MS.gene073154	PPI
MS.gene51432	MS.gene35285	PPI
MS.gene51432	MS.gene73865	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51432.t1	MTR_6g086280	97.260	146	4	0	1	146	4	149	1.78e-100	285
MS.gene51432.t1	MTR_6g086280	97.222	144	4	0	1	144	4	147	4.12e-99	281
MS.gene51432.t1	MTR_2g084220	50.000	138	69	0	6	143	2	139	4.64e-43	139
MS.gene51432.t1	MTR_4g080090	50.725	138	68	0	6	143	4	141	9.39e-42	136
MS.gene51432.t1	MTR_6g018330	42.105	133	61	1	11	143	26	142	1.70e-26	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51432.t1	AT3G24500	71.622	148	40	1	1	146	1	148	9.93e-69	204
MS.gene51432.t1	AT3G58680	52.174	138	66	0	6	143	4	141	4.19e-44	142
MS.gene51432.t1	AT2G42680	50.725	138	68	0	6	143	4	141	7.40e-43	139
MS.gene51432.t1	AT3G24500	58.947	95	37	1	1	93	1	95	8.16e-33	112

Find 43 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGGAAAATGGAGAAGGTTT+TGG	0.237217	6.2:+84681591	MS.gene51432:CDS
TGTTATTAATCAGGGTGTTT+TGG	0.262576	6.2:+84681570	MS.gene51432:CDS
GTTAAACTTAGGGGTAAAAT+TGG	0.277746	6.2:+84681616	MS.gene51432:CDS
AGGGCTTGGTTTATTGCCTT+TGG	0.277912	6.2:-84681295	None:intergenic
CTGCTCCGGTTCGGAGGGCT+TGG	0.299889	6.2:-84681309	None:intergenic
TTACGAAGGTCTTGAGCTTT+GGG	0.313356	6.2:-84681271	None:intergenic
TGAAAGGGTTCAGGTTGTTC+AGG	0.318932	6.2:+84681528	MS.gene51432:CDS
CGCGATACAGAAAGCGCGTT+TGG	0.332684	6.2:+84681465	MS.gene51432:CDS
GTTACGAAGGTCTTGAGCTT+TGG	0.336478	6.2:-84681272	None:intergenic
ATTGATTAATGAAAGGGTTC+AGG	0.341931	6.2:+84681519	MS.gene51432:CDS
TGGTAAAGCTGTTATTAATC+AGG	0.346445	6.2:+84681561	MS.gene51432:CDS
GAAGATGAGTCAGGGTGATT+TGG	0.359012	6.2:+84681492	MS.gene51432:CDS
GGGCTTGGTTTATTGCCTTT+GGG	0.364374	6.2:-84681294	None:intergenic
AGCTTCCTCGCGTTTAAAAC+CGG	0.385062	6.2:-84681388	None:intergenic
GGCGAAATTGATTAATGAAA+GGG	0.407053	6.2:+84681513	MS.gene51432:CDS
CTGAACCAGCAGCGTTGGAG+CGG	0.424927	6.2:+84681422	MS.gene51432:CDS
AAGGTCTTGAGCTTTGGGTT+TGG	0.439281	6.2:-84681266	None:intergenic
TAGGAACAATAAAACAAGAC+TGG	0.459948	6.2:+84681221	MS.gene51432:CDS
GCAGCGTTGGAGCGGGTTGG+TGG	0.474323	6.2:+84681430	MS.gene51432:CDS
TGGCGAAATTGATTAATGAA+AGG	0.476549	6.2:+84681512	MS.gene51432:CDS
TTTGGATAAGAAGATGAGTC+AGG	0.478923	6.2:+84681483	MS.gene51432:CDS
ATAAACCAAGCCCTCCGAAC+CGG	0.491593	6.2:+84681304	MS.gene51432:CDS
AGCGGCTGAACCAGCAGCGT+TGG	0.495442	6.2:+84681417	MS.gene51432:CDS
GACTGTTAAGACCTCTGCTC+CGG	0.499262	6.2:-84681323	None:intergenic
TTTAAAACCGGTCCGCTCGT+TGG	0.502945	6.2:-84681376	None:intergenic
TTAAGACCTCTGCTCCGGTT+CGG	0.505245	6.2:-84681318	None:intergenic
CCAACCCGCTCCAACGCTGC+TGG	0.509376	6.2:-84681427	None:intergenic
GACCTCTGCTCCGGTTCGGA+GGG	0.511130	6.2:-84681314	None:intergenic
TGAACCAGCAGCGTTGGAGC+GGG	0.514144	6.2:+84681423	MS.gene51432:CDS
AGACCTCTGCTCCGGTTCGG+AGG	0.519624	6.2:-84681315	None:intergenic
ATTGTTCCTACTGTTCGTGT+CGG	0.524804	6.2:-84681208	None:intergenic
GGTAAAGCTGTTATTAATCA+GGG	0.553453	6.2:+84681562	MS.gene51432:CDS
CGCGAGGAAGCTAGATGAAG+CGG	0.564479	6.2:+84681399	MS.gene51432:CDS
GTTGTTCAGGAGTATGAGAA+TGG	0.565499	6.2:+84681541	MS.gene51432:CDS
AGCCCTCCGAACCGGAGCAG+AGG	0.582656	6.2:+84681312	MS.gene51432:CDS
CCAGCAGCGTTGGAGCGGGT+TGG	0.583304	6.2:+84681427	MS.gene51432:CDS
ACGATGCCGACACGAACAGT+AGG	0.590972	6.2:+84681202	None:intergenic
TTATTGCCTTTGGGTTACGA+AGG	0.607239	6.2:-84681285	None:intergenic
AGGAACAATAAAACAAGACT+GGG	0.609753	6.2:+84681222	MS.gene51432:CDS
AAAACAAGACTGGGAACCCG+TGG	0.638139	6.2:+84681231	MS.gene51432:CDS
ACAGTCAAGAAACCAACAGC+CGG	0.641212	6.2:+84681340	MS.gene51432:CDS
TTGGATAAGAAGATGAGTCA+GGG	0.654305	6.2:+84681484	MS.gene51432:CDS
TCAAGACCTTCGTAACCCAA+AGG	0.668433	6.2:+84681279	MS.gene51432:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGAACAATAAAACAAGACT+GGG	+	chr6.2:84681222-84681241	MS.gene51432:CDS	30.0%
	ATTGATTAATGAAAGGGTTC+AGG	+	chr6.2:84681519-84681538	MS.gene51432:CDS	30.0%
	GGCGAAATTGATTAATGAAA+GGG	+	chr6.2:84681513-84681532	MS.gene51432:CDS	30.0%
	GGTAAAGCTGTTATTAATCA+GGG	+	chr6.2:84681562-84681581	MS.gene51432:CDS	30.0%
	GTTAAACTTAGGGGTAAAAT+TGG	+	chr6.2:84681616-84681635	MS.gene51432:CDS	30.0%
	TAGGAACAATAAAACAAGAC+TGG	+	chr6.2:84681221-84681240	MS.gene51432:CDS	30.0%
	TGGCGAAATTGATTAATGAA+AGG	+	chr6.2:84681512-84681531	MS.gene51432:CDS	30.0%
	TGGTAAAGCTGTTATTAATC+AGG	+	chr6.2:84681561-84681580	MS.gene51432:CDS	30.0%
!!	GTTATTAATCAGGGTGTTTT+GGG	+	chr6.2:84681571-84681590	MS.gene51432:CDS	30.0%
!!	TGTTATTAATCAGGGTGTTT+TGG	+	chr6.2:84681570-84681589	MS.gene51432:CDS	30.0%
!!	TTATTAATCAGGGTGTTTTG+GGG	+	chr6.2:84681572-84681591	MS.gene51432:CDS	30.0%
	TTGGATAAGAAGATGAGTCA+GGG	+	chr6.2:84681484-84681503	MS.gene51432:CDS	35.0%
	TTTGGATAAGAAGATGAGTC+AGG	+	chr6.2:84681483-84681502	MS.gene51432:CDS	35.0%
	ATTGTTCCTACTGTTCGTGT+CGG	-	chr6.2:84681211-84681230	None:intergenic	40.0%
	TCGAACAAAAAACCAACGAG+CGG	+	chr6.2:84681364-84681383	MS.gene51432:CDS	40.0%
	TTACGAAGGTCTTGAGCTTT+GGG	-	chr6.2:84681274-84681293	None:intergenic	40.0%
!	GGAAAATGGAGAAGGTTTTG+GGG	+	chr6.2:84681593-84681612	MS.gene51432:CDS	40.0%
!	GGGAAAATGGAGAAGGTTTT+GGG	+	chr6.2:84681592-84681611	MS.gene51432:CDS	40.0%
!	GTTGTTCAGGAGTATGAGAA+TGG	+	chr6.2:84681541-84681560	MS.gene51432:CDS	40.0%
!	TTATTGCCTTTGGGTTACGA+AGG	-	chr6.2:84681288-84681307	None:intergenic	40.0%
!!	GAAAATGGAGAAGGTTTTGG+GGG	+	chr6.2:84681594-84681613	MS.gene51432:CDS	40.0%
!!!	AGGTTTTGGGGGTTAAACTT+AGG	+	chr6.2:84681605-84681624	MS.gene51432:CDS	40.0%
!!!	CGTTGGTTTTTTGTTCGAAC+CGG	-	chr6.2:84681362-84681381	None:intergenic	40.0%
!!!	GGTTTTGGGGGTTAAACTTA+GGG	+	chr6.2:84681606-84681625	MS.gene51432:CDS	40.0%
!!!	GTTTTGGGGGTTAAACTTAG+GGG	+	chr6.2:84681607-84681626	MS.gene51432:CDS	40.0%
!!!	TGTTTTGGGGAAAATGGAGA+AGG	+	chr6.2:84681585-84681604	MS.gene51432:CDS	40.0%
!!!	TTTGGTTTTGTGGAGAACCA+CGG	-	chr6.2:84681251-84681270	None:intergenic	40.0%
	ACAGTCAAGAAACCAACAGC+CGG	+	chr6.2:84681340-84681359	MS.gene51432:CDS	45.0%
	GAAGATGAGTCAGGGTGATT+TGG	+	chr6.2:84681492-84681511	MS.gene51432:CDS	45.0%
	GTTACGAAGGTCTTGAGCTT+TGG	-	chr6.2:84681275-84681294	None:intergenic	45.0%
	TCAAGACCTTCGTAACCCAA+AGG	+	chr6.2:84681279-84681298	MS.gene51432:CDS	45.0%
!	AGCTTCCTCGCGTTTAAAAC+CGG	-	chr6.2:84681391-84681410	None:intergenic	45.0%
!	AGGGCTTGGTTTATTGCCTT+TGG	-	chr6.2:84681298-84681317	None:intergenic	45.0%
!	GGGCTTGGTTTATTGCCTTT+GGG	-	chr6.2:84681297-84681316	None:intergenic	45.0%
!	GGGGAAAATGGAGAAGGTTT+TGG	+	chr6.2:84681591-84681610	MS.gene51432:CDS	45.0%
!	TTTTTGTTCGAACCGGCTGT+TGG	-	chr6.2:84681355-84681374	None:intergenic	45.0%
!!	AAGGTCTTGAGCTTTGGGTT+TGG	-	chr6.2:84681269-84681288	None:intergenic	45.0%
!!	TGAAAGGGTTCAGGTTGTTC+AGG	+	chr6.2:84681528-84681547	MS.gene51432:CDS	45.0%
!!!	GAGCTTTGGGTTTGGTTTTG+TGG	-	chr6.2:84681261-84681280	None:intergenic	45.0%
!!!	TCAGGGTGTTTTGGGGAAAA+TGG	+	chr6.2:84681579-84681598	MS.gene51432:CDS	45.0%
!!!	TTGGTTTTGTGGAGAACCAC+GGG	-	chr6.2:84681250-84681269	None:intergenic	45.0%
	AAAACAAGACTGGGAACCCG+TGG	+	chr6.2:84681231-84681250	MS.gene51432:CDS	50.0%
	ATAAACCAAGCCCTCCGAAC+CGG	+	chr6.2:84681304-84681323	MS.gene51432:CDS	50.0%
	CAAAAAACCAACGAGCGGAC+CGG	+	chr6.2:84681369-84681388	MS.gene51432:CDS	50.0%
	GACTGTTAAGACCTCTGCTC+CGG	-	chr6.2:84681326-84681345	None:intergenic	50.0%
	TTAAGACCTCTGCTCCGGTT+CGG	-	chr6.2:84681321-84681340	None:intergenic	50.0%
!	TTTAAAACCGGTCCGCTCGT+TGG	-	chr6.2:84681379-84681398	None:intergenic	50.0%
	CGCGAGGAAGCTAGATGAAG+CGG	+	chr6.2:84681399-84681418	MS.gene51432:CDS	55.0%
!	CGCGATACAGAAAGCGCGTT+TGG	+	chr6.2:84681465-84681484	MS.gene51432:CDS	55.0%
!	CTGAACCAGCAGCGTTGGAG+CGG	+	chr6.2:84681422-84681441	MS.gene51432:CDS	60.0%
!	TGAACCAGCAGCGTTGGAGC+GGG	+	chr6.2:84681423-84681442	MS.gene51432:CDS	60.0%
!!!	GCGGACCGGTTTTAAACGCG+AGG	+	chr6.2:84681383-84681402	MS.gene51432:CDS	60.0%
	AGCGGCTGAACCAGCAGCGT+TGG	+	chr6.2:84681417-84681436	MS.gene51432:CDS	65.0%
!!	AGACCTCTGCTCCGGTTCGG+AGG	-	chr6.2:84681318-84681337	None:intergenic	65.0%
!!	GACCTCTGCTCCGGTTCGGA+GGG	-	chr6.2:84681317-84681336	None:intergenic	65.0%
	AGCCCTCCGAACCGGAGCAG+AGG	+	chr6.2:84681312-84681331	MS.gene51432:CDS	70.0%
	CCAACCCGCTCCAACGCTGC+TGG	-	chr6.2:84681430-84681449	None:intergenic	70.0%
!	CCAGCAGCGTTGGAGCGGGT+TGG	+	chr6.2:84681427-84681446	MS.gene51432:CDS	70.0%
!!	CTGCTCCGGTTCGGAGGGCT+TGG	-	chr6.2:84681312-84681331	None:intergenic	70.0%
!!	GCAGCGTTGGAGCGGGTTGG+TGG	+	chr6.2:84681430-84681449	MS.gene51432:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	84681205	84681645	84681205	ID=MS.gene51432
chr6.2	mRNA	84681205	84681645	84681205	ID=MS.gene51432.t1;Parent=MS.gene51432
chr6.2	exon	84681205	84681645	84681205	ID=MS.gene51432.t1.exon1;Parent=MS.gene51432.t1
chr6.2	CDS	84681205	84681645	84681205	ID=cds.MS.gene51432.t1;Parent=MS.gene51432.t1

Gene Sequence

>MS.gene51432

ATGCCGACACGAACAGTAGGAACAATAAAACAAGACTGGGAACCCGTGGTTCTCCACAAAACCAAACCCAAAGCTCAAGACCTTCGTAACCCAAAGGCAATAAACCAAGCCCTCCGAACCGGAGCAGAGGTCTTAACAGTCAAGAAACCAACAGCCGGTTCGAACAAAAAACCAACGAGCGGACCGGTTTTAAACGCGAGGAAGCTAGATGAAGCGGCTGAACCAGCAGCGTTGGAGCGGGTTGGTGGAGAAGTGAGACACGCGATACAGAAAGCGCGTTTGGATAAGAAGATGAGTCAGGGTGATTTGGCGAAATTGATTAATGAAAGGGTTCAGGTTGTTCAGGAGTATGAGAATGGTAAAGCTGTTATTAATCAGGGTGTTTTGGGGAAAATGGAGAAGGTTTTGGGGGTTAAACTTAGGGGTAAAATTGGAAAATAA

Protein sequence

>MS.gene51432.t1

MPTRTVGTIKQDWEPVVLHKTKPKAQDLRNPKAINQALRTGAEVLTVKKPTAGSNKKPTSGPVLNARKLDEAAEPAALERVGGEVRHAIQKARLDKKMSQGDLAKLINERVQVVQEYENGKAVINQGVLGKMEKVLGVKLRGKIGK