Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056171.t1 | XP_013445072.1 | 99.6 | 464 | 2 | 0 | 43 | 506 | 34 | 497 | 7.40E-274 | 952.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056171.t1 | Q6DKP5 | 37.0 | 330 | 198 | 7 | 177 | 499 | 158 | 484 | 3.8e-53 | 210.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056171.t1 | A0A072TQH3 | 99.6 | 464 | 2 | 0 | 43 | 506 | 34 | 497 | 5.3e-274 | 952.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene056171 | MS.gene036937 | PPI |
| MS.gene056171 | MS.gene51850 | PPI |
| MS.gene056171 | MS.gene022257 | PPI |
| MS.gene056171 | MS.gene074392 | PPI |
| MS.gene056171 | MS.gene27507 | PPI |
| MS.gene056171 | MS.gene70587 | PPI |
| MS.gene056171 | MS.gene87680 | PPI |
| MS.gene056171 | MS.gene92768 | PPI |
| MS.gene056171 | MS.gene25914 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056171.t1 | MTR_8g037340 | 96.640 | 506 | 8 | 1 | 1 | 506 | 1 | 497 | 0.0 | 1011 |
| MS.gene056171.t1 | MTR_8g037340 | 91.231 | 536 | 8 | 2 | 1 | 506 | 1 | 527 | 0.0 | 935 |
| MS.gene056171.t1 | MTR_1g072140 | 28.182 | 330 | 205 | 13 | 175 | 498 | 12 | 315 | 3.48e-25 | 106 |
| MS.gene056171.t1 | MTR_4g119620 | 27.301 | 326 | 194 | 15 | 190 | 499 | 27 | 325 | 6.93e-19 | 88.2 |
| MS.gene056171.t1 | MTR_4g123597 | 21.557 | 334 | 227 | 8 | 190 | 495 | 106 | 432 | 1.18e-11 | 67.8 |
| MS.gene056171.t1 | MTR_2g079740 | 22.887 | 284 | 183 | 8 | 217 | 482 | 474 | 739 | 3.21e-11 | 66.6 |
| MS.gene056171.t1 | MTR_8g092890 | 21.849 | 238 | 167 | 6 | 217 | 453 | 276 | 495 | 5.66e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056171.t1 | AT2G16405 | 72.671 | 483 | 127 | 4 | 20 | 501 | 3 | 481 | 0.0 | 734 |
| MS.gene056171.t1 | AT3G49660 | 27.329 | 322 | 189 | 16 | 192 | 499 | 26 | 316 | 3.22e-21 | 94.7 |
| MS.gene056171.t1 | AT4G02730 | 25.316 | 316 | 201 | 14 | 191 | 498 | 44 | 332 | 4.16e-17 | 82.8 |
| MS.gene056171.t1 | AT5G54200 | 25.207 | 242 | 153 | 9 | 217 | 452 | 453 | 672 | 3.51e-12 | 69.7 |
| MS.gene056171.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 217 | 451 | 313 | 530 | 4.44e-12 | 68.9 |
| MS.gene056171.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 217 | 451 | 313 | 530 | 4.44e-12 | 68.9 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTCTTTCTCTTGAAAA+AGG | 0.184205 | 8.1:-65974517 | MS.gene056171:CDS |
| GAGGTGGACAGCTGGAGTTC+TGG | 0.218453 | 8.1:-65979202 | MS.gene056171:CDS |
| AGTTCTGACCTACTTGCTTA+TGG | 0.246804 | 8.1:-65978196 | MS.gene056171:CDS |
| TGGATAAAACTGTGAGAGTA+TGG | 0.287100 | 8.1:-65976876 | MS.gene056171:CDS |
| GTACATTGCTTCTGCTTCAT+TGG | 0.327705 | 8.1:-65976896 | MS.gene056171:CDS |
| AATTGCATGCGGGTGATATA+TGG | 0.338828 | 8.1:-65976838 | MS.gene056171:CDS |
| GTAGTTACGGTGATAAAGTT+TGG | 0.339409 | 8.1:-65978226 | MS.gene056171:intron |
| AGGTGGACAGCTGGAGTTCT+GGG | 0.349940 | 8.1:-65979201 | MS.gene056171:CDS |
| CCCGGAGTACTTCGAAGACT+TGG | 0.357960 | 8.1:+65979912 | None:intergenic |
| GGCATATTACAGTGTGGCTT+TGG | 0.361902 | 8.1:-65974617 | MS.gene056171:intron |
| ACATAAACAGGCCTAGGAAC+TGG | 0.364322 | 8.1:-65979953 | MS.gene056171:CDS |
| TTGTGGTTGGTGGTGGTTGA+CGG | 0.364670 | 8.1:+65981125 | None:intergenic |
| AGAGTATGGGAGATAGAAAA+AGG | 0.367149 | 8.1:-65976862 | MS.gene056171:CDS |
| TAGGCCTGTTTATGTAATTC+CGG | 0.374723 | 8.1:+65979961 | None:intergenic |
| TGACGCCGAAGTTACCTCTA+TGG | 0.376140 | 8.1:-65975044 | MS.gene056171:CDS |
| TCCAATCATAGAGCAAGTTT+TGG | 0.376757 | 8.1:-65978452 | MS.gene056171:CDS |
| TTTACGGCATAGTAATTGTT+TGG | 0.377298 | 8.1:-65974097 | MS.gene056171:CDS |
| AAATTCAGCAACCTGTTTCC+CGG | 0.380388 | 8.1:+65979894 | None:intergenic |
| GTTCTGACCTACTTGCTTAT+GGG | 0.394653 | 8.1:-65978195 | MS.gene056171:CDS |
| CAGTCACAACAGTGCAGTAT+AGG | 0.394700 | 8.1:-65974800 | MS.gene056171:CDS |
| GGTGTTGCGGTTGTGGTTGG+TGG | 0.400969 | 8.1:+65981115 | None:intergenic |
| CCTAGGAACTGGGAAAGTTT+AGG | 0.402238 | 8.1:-65979942 | MS.gene056171:CDS |
| TTGTTCACACATGTATGCTT+TGG | 0.408174 | 8.1:+65974266 | None:intergenic |
| TTCGAAGTACTCCGGGAAAC+AGG | 0.408718 | 8.1:-65979905 | MS.gene056171:intron |
| AGTGTGGCTTTGGAGATAAA+AGG | 0.415343 | 8.1:-65974607 | MS.gene056171:CDS |
| GCCAAAACTTGCTCTATGAT+TGG | 0.428070 | 8.1:+65978451 | None:intergenic |
| CCGGAGTACTTCGAAGACTT+GGG | 0.432049 | 8.1:+65979913 | None:intergenic |
| GGGTCATCGGTTTCCTGTGA+TGG | 0.438655 | 8.1:-65974165 | MS.gene056171:CDS |
| AAGCAGGAGGCGACGAATGC+CGG | 0.439791 | 8.1:+65980966 | None:intergenic |
| GGTGGTGTTGCGGTTGTGGT+TGG | 0.439985 | 8.1:+65981112 | None:intergenic |
| TAGTTACGGTGATAAAGTTT+GGG | 0.440842 | 8.1:-65978225 | MS.gene056171:CDS |
| AGAAGACAGCAGAATAAGTC+TGG | 0.447786 | 8.1:+65981024 | None:intergenic |
| GTTGCGGTTGTGGTTGGTGG+TGG | 0.448576 | 8.1:+65981118 | None:intergenic |
| TCTGATCGTCCACGAATTCG+TGG | 0.450477 | 8.1:-65978407 | MS.gene056171:CDS |
| GACCAACGGGAGAGTGGACT+TGG | 0.450591 | 8.1:+65979226 | None:intergenic |
| AAAATATGAGATGGCCAGTA+TGG | 0.452859 | 8.1:+65975014 | None:intergenic |
| ATGCATTCCTACGAAGGAAT+AGG | 0.453630 | 8.1:+65978373 | None:intergenic |
| CATACCGGAATTACATAAAC+AGG | 0.453670 | 8.1:-65979965 | MS.gene056171:CDS |
| CCTAAACTTTCCCAGTTCCT+AGG | 0.455108 | 8.1:+65979942 | None:intergenic |
| TTCCTGTGATGGGGATTGCT+TGG | 0.458213 | 8.1:-65974154 | MS.gene056171:CDS |
| GGTCATCGGTTTCCTGTGAT+GGG | 0.461880 | 8.1:-65974164 | MS.gene056171:CDS |
| AATAACAACTTCCTTTCTGT+TGG | 0.467290 | 8.1:-65975873 | MS.gene056171:CDS |
| GTTGGTCTGAGGTGGACAGC+TGG | 0.471148 | 8.1:-65979210 | MS.gene056171:CDS |
| TCTATGGACCACGACCATAC+TGG | 0.474694 | 8.1:-65975028 | MS.gene056171:CDS |
| GCTGTCCACCTCAGACCAAC+GGG | 0.477029 | 8.1:+65979213 | None:intergenic |
| ACTTCGATGACGATGTGATC+GGG | 0.477217 | 8.1:+65974842 | None:intergenic |
| TCAGTCATCAAACAATTAAA+CGG | 0.482319 | 8.1:-65978127 | MS.gene056171:CDS |
| TTCGACCAGTGCAAAGCCTC+TGG | 0.486329 | 8.1:-65978344 | MS.gene056171:intron |
| TTGTACTCACAGGGTGGAAA+CGG | 0.487559 | 8.1:+65976794 | None:intergenic |
| TGGTTGGTGGTGGTTGACGG+TGG | 0.495026 | 8.1:+65981128 | None:intergenic |
| CGGGAGAGTGGACTTGGAGA+TGG | 0.503119 | 8.1:+65979232 | None:intergenic |
| TGGATACTGAATTTGCAACT+TGG | 0.513649 | 8.1:+65978471 | None:intergenic |
| CTATACTGCACTGTTGTGAC+TGG | 0.513927 | 8.1:+65974801 | None:intergenic |
| TACTTCGATGACGATGTGAT+CGG | 0.514557 | 8.1:+65974841 | None:intergenic |
| TTTAATTGTTTGATGACTGA+AGG | 0.515062 | 8.1:+65978130 | None:intergenic |
| CTTGCTTATGGGGCATCAGA+TGG | 0.516204 | 8.1:-65978184 | MS.gene056171:CDS |
| TAGATTTCCATCCTGAGTAC+AGG | 0.521762 | 8.1:+65974740 | None:intergenic |
| CCCAAGTCTTCGAAGTACTC+CGG | 0.525236 | 8.1:-65979913 | MS.gene056171:CDS |
| GGAAAACTTACGTGGCATAC+CGG | 0.525881 | 8.1:-65979980 | MS.gene056171:CDS |
| GAGCCTGCCTATTCCTTCGT+AGG | 0.527204 | 8.1:-65978380 | MS.gene056171:CDS |
| CGACGAATGCCGGTGTACTC+AGG | 0.531207 | 8.1:+65980976 | None:intergenic |
| CATAAACAGGCCTAGGAACT+GGG | 0.532543 | 8.1:-65979952 | MS.gene056171:CDS |
| TTCTGACCTACTTGCTTATG+GGG | 0.543681 | 8.1:-65978194 | MS.gene056171:CDS |
| TACCTGTGATGTCTTTCGAA+TGG | 0.549760 | 8.1:+65978104 | None:intergenic |
| AGCTGGAATTCTCCGCCGTA+AGG | 0.553349 | 8.1:-65980929 | MS.gene056171:intron |
| TCCACTCTCCCGTTGGTCTG+AGG | 0.553452 | 8.1:-65979221 | MS.gene056171:CDS |
| CTCCAAGTCCACTCTCCCGT+TGG | 0.556019 | 8.1:-65979228 | MS.gene056171:CDS |
| ACTCTCCCGTTGGTCTGAGG+TGG | 0.557213 | 8.1:-65979218 | MS.gene056171:CDS |
| TGTTTGCATTGCCAACAGAA+AGG | 0.557295 | 8.1:+65975862 | None:intergenic |
| GGCTCAACACCACGAATTCG+TGG | 0.557690 | 8.1:+65978398 | None:intergenic |
| TGTGTTGTTGACCTGTACTC+AGG | 0.559101 | 8.1:-65974751 | MS.gene056171:CDS |
| AGCTGTCCACCTCAGACCAA+CGG | 0.563191 | 8.1:+65979212 | None:intergenic |
| TGAAGGATTGGAGATGCAAT+GGG | 0.565209 | 8.1:-65980001 | MS.gene056171:intron |
| CCAAGTCTTCGAAGTACTCC+GGG | 0.573735 | 8.1:-65979912 | MS.gene056171:CDS |
| CGGAGTACTTCGAAGACTTG+GGG | 0.573894 | 8.1:+65979914 | None:intergenic |
| TTCCAAGCAATCCCCATCAC+AGG | 0.577118 | 8.1:+65974152 | None:intergenic |
| TACATGTGTGAACAAGCTAC+AGG | 0.579845 | 8.1:-65974258 | MS.gene056171:intron |
| TTGAAGGATTGGAGATGCAA+TGG | 0.580876 | 8.1:-65980002 | MS.gene056171:intron |
| TCCATCACCATGGCGGAGCA+CGG | 0.582837 | 8.1:-65981156 | None:intergenic |
| TACTACCAGAGGCTTTGCAC+TGG | 0.583440 | 8.1:+65978339 | None:intergenic |
| ATGGGGATTGCTTGGAACCA+TGG | 0.583684 | 8.1:-65974146 | MS.gene056171:CDS |
| TGAAACACTGCACACAGTCA+AGG | 0.586692 | 8.1:+65978159 | None:intergenic |
| GGATAAAACTGTGAGAGTAT+GGG | 0.586995 | 8.1:-65976875 | MS.gene056171:CDS |
| TTGGTGGTGGTTGACGGTGG+AGG | 0.587897 | 8.1:+65981131 | None:intergenic |
| CTGATGCCCCATAAGCAAGT+AGG | 0.590315 | 8.1:+65978188 | None:intergenic |
| GGATCAGCGTCGGAGGTGAG+AGG | 0.592584 | 8.1:+65980997 | None:intergenic |
| TGAGATGGCCAGTATGGTCG+TGG | 0.593850 | 8.1:+65975020 | None:intergenic |
| ACCTCAGACCAACGGGAGAG+TGG | 0.599253 | 8.1:+65979220 | None:intergenic |
| GGACAGCTGGAGTTCTGGGA+GGG | 0.602771 | 8.1:-65979197 | MS.gene056171:intron |
| CATCTCCACAAAATATGAGA+TGG | 0.602782 | 8.1:+65975005 | None:intergenic |
| GCTCGCTCAAGTTAACTCCT+CGG | 0.603557 | 8.1:-65974567 | MS.gene056171:CDS |
| GGAATTACATAAACAGGCCT+AGG | 0.605131 | 8.1:-65979959 | MS.gene056171:CDS |
| GATGCAATGCATTCCTACGA+AGG | 0.607558 | 8.1:+65978367 | None:intergenic |
| TGAGTACAGGTCAACAACAC+AGG | 0.611720 | 8.1:+65974753 | None:intergenic |
| TCGGGATAATATACCTGTGT+GGG | 0.612554 | 8.1:+65974860 | None:intergenic |
| ATTCCTACGAAGGAATAGGC+AGG | 0.617187 | 8.1:+65978377 | None:intergenic |
| GTCATCGGTTTCCTGTGATG+GGG | 0.617591 | 8.1:-65974163 | MS.gene056171:CDS |
| AGTATGGTCGTGGTCCATAG+AGG | 0.617688 | 8.1:+65975030 | None:intergenic |
| AGTTTCTCTCTGCTAGCTCG+AGG | 0.630037 | 8.1:-65974777 | MS.gene056171:CDS |
| GTTGATGCATCAGTGGCCGA+TGG | 0.633147 | 8.1:-65979258 | MS.gene056171:intron |
| GGTGTACTCAGGATCAGCGT+CGG | 0.640899 | 8.1:+65980987 | None:intergenic |
| GTGGTCCATAGAGGTAACTT+CGG | 0.643822 | 8.1:+65975039 | None:intergenic |
| TGGACAGCTGGAGTTCTGGG+AGG | 0.650084 | 8.1:-65979198 | MS.gene056171:CDS |
| TTGTTGACCTGTACTCAGGA+TGG | 0.657122 | 8.1:-65974747 | MS.gene056171:CDS |
| GTAAATATGAACTCCCACAC+AGG | 0.663031 | 8.1:-65974873 | MS.gene056171:CDS |
| GGCCATTCGAAAGACATCAC+AGG | 0.665122 | 8.1:-65978106 | MS.gene056171:intron |
| GTACTCAGGATCAGCGTCGG+AGG | 0.665749 | 8.1:+65980990 | None:intergenic |
| ATATGCAGTTGCTGGAAGTG+AGG | 0.669433 | 8.1:-65974321 | MS.gene056171:intron |
| ATCGGGATAATATACCTGTG+TGG | 0.691235 | 8.1:+65974859 | None:intergenic |
| GACTTGGAGATGGAAACCAT+CGG | 0.697706 | 8.1:+65979242 | None:intergenic |
| ATGCAATGGGAAAACTTACG+TGG | 0.711127 | 8.1:-65979988 | MS.gene056171:CDS |
| CAACAACAATATCGACGACG+AGG | 0.716001 | 8.1:-65981073 | MS.gene056171:CDS |
| GACGCTGATCCTGAGTACAC+CGG | 0.736062 | 8.1:-65980985 | MS.gene056171:CDS |
| ATGCGGAGTATACCTTACGG+CGG | 0.798435 | 8.1:+65980917 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 65974061 | 65981169 | 65974061 | ID=MS.gene056171 |
| chr8.1 | mRNA | 65974061 | 65981169 | 65974061 | ID=MS.gene056171.t1;Parent=MS.gene056171 |
| chr8.1 | exon | 65980930 | 65981169 | 65980930 | ID=MS.gene056171.t1.exon1;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65980930 | 65981169 | 65980930 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65979906 | 65980018 | 65979906 | ID=MS.gene056171.t1.exon2;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65979906 | 65980018 | 65979906 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65979198 | 65979267 | 65979198 | ID=MS.gene056171.t1.exon3;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65979198 | 65979267 | 65979198 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65978345 | 65978498 | 65978345 | ID=MS.gene056171.t1.exon4;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65978345 | 65978498 | 65978345 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65978107 | 65978244 | 65978107 | ID=MS.gene056171.t1.exon5;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65978107 | 65978244 | 65978107 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65976804 | 65976943 | 65976804 | ID=MS.gene056171.t1.exon6;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65976804 | 65976943 | 65976804 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65975851 | 65975898 | 65975851 | ID=MS.gene056171.t1.exon7;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65975851 | 65975898 | 65975851 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65975000 | 65975110 | 65975000 | ID=MS.gene056171.t1.exon8;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65975000 | 65975110 | 65975000 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65974729 | 65974910 | 65974729 | ID=MS.gene056171.t1.exon9;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65974729 | 65974910 | 65974729 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65974506 | 65974627 | 65974506 | ID=MS.gene056171.t1.exon10;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65974506 | 65974627 | 65974506 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65974259 | 65974335 | 65974259 | ID=MS.gene056171.t1.exon11;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65974259 | 65974335 | 65974259 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| chr8.1 | exon | 65974061 | 65974186 | 65974061 | ID=MS.gene056171.t1.exon12;Parent=MS.gene056171.t1 |
| chr8.1 | CDS | 65974061 | 65974186 | 65974061 | ID=cds.MS.gene056171.t1;Parent=MS.gene056171.t1 |
| Gene Sequence |
| Protein sequence |
