Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51850.t1 | XP_024626163.1 | 97.1 | 175 | 5 | 0 | 507 | 681 | 662 | 836 | 4.30E-83 | 319.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51850.t1 | A0A072U0S8 | 97.1 | 173 | 5 | 0 | 509 | 681 | 739 | 911 | 4.5e-82 | 315.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene51850 | 0.81122 | 7.81E-51 | -1.69E-46 |
| MS.gene049159 | MS.gene51850 | 0.819723 | 1.00E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene51850 | 0.827488 | 1.52E-54 | -1.69E-46 |
| MS.gene049647 | MS.gene51850 | 0.802143 | 6.45E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene51850 | 0.801759 | 7.74E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene51850 | 0.850629 | 1.50E-60 | -1.69E-46 |
| MS.gene050721 | MS.gene51850 | 0.85426 | 1.39E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene51850 | 0.849657 | 2.81E-60 | -1.69E-46 |
| MS.gene050815 | MS.gene51850 | 0.820554 | 6.45E-53 | -1.69E-46 |
| MS.gene050824 | MS.gene51850 | 0.831503 | 1.61E-55 | -1.69E-46 |
| MS.gene051247 | MS.gene51850 | 0.801403 | 9.15E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene51850 | 0.829435 | 5.16E-55 | -1.69E-46 |
| MS.gene052020 | MS.gene51850 | 0.807403 | 5.14E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene51850 | 0.808184 | 3.51E-50 | -1.69E-46 |
| MS.gene052856 | MS.gene51850 | 0.808991 | 2.36E-50 | -1.69E-46 |
| MS.gene052858 | MS.gene51850 | 0.801585 | 8.40E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene51850 | 0.824872 | 6.38E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene51850 | 0.838375 | 2.98E-57 | -1.69E-46 |
| MS.gene053008 | MS.gene51850 | 0.819011 | 1.45E-52 | -1.69E-46 |
| MS.gene053381 | MS.gene51850 | 0.804192 | 2.43E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene51850 | 0.817248 | 3.64E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene51850 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene51850 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene51850 | 0.804399 | 2.20E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene51850 | 0.807419 | 5.10E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene51850 | 0.806402 | 8.37E-50 | -1.69E-46 |
| MS.gene056043 | MS.gene51850 | -0.804983 | 1.66E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene51850 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene51850 | 0.800298 | 1.54E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene51850 | 0.850734 | 1.40E-60 | -1.69E-46 |
| MS.gene057980 | MS.gene51850 | 0.810219 | 1.29E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene51850 | 0.824625 | 7.29E-54 | -1.69E-46 |
| MS.gene058636 | MS.gene51850 | 0.815166 | 1.06E-51 | -1.69E-46 |
| MS.gene058851 | MS.gene51850 | 0.811517 | 6.73E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene51850 | 0.85629 | 3.56E-62 | -1.69E-46 |
| MS.gene059249 | MS.gene51850 | 0.811084 | 8.36E-51 | -1.69E-46 |
| MS.gene059937 | MS.gene51850 | 0.8099 | 1.51E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene51850 | 0.806382 | 8.45E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene51850 | 0.81462 | 1.40E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene51850 | 0.801772 | 7.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51850 | MS.gene074392 | PPI |
| MS.gene51850 | MS.gene060358 | PPI |
| MS.gene45751 | MS.gene51850 | PPI |
| MS.gene036548 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene29263 | PPI |
| MS.gene51850 | MS.gene033811 | PPI |
| MS.gene056171 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene70587 | PPI |
| MS.gene002806 | MS.gene51850 | PPI |
| MS.gene022257 | MS.gene51850 | PPI |
| MS.gene041500 | MS.gene51850 | PPI |
| MS.gene007439 | MS.gene51850 | PPI |
| MS.gene016665 | MS.gene51850 | PPI |
| MS.gene049813 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene067126 | PPI |
| MS.gene51850 | MS.gene75814 | PPI |
| MS.gene88572 | MS.gene51850 | PPI |
| MS.gene97056 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene00961 | PPI |
| MS.gene51850 | MS.gene22441 | PPI |
| MS.gene51850 | MS.gene91079 | PPI |
| MS.gene007508 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene25914 | PPI |
| MS.gene20056 | MS.gene51850 | PPI |
| MS.gene004491 | MS.gene51850 | PPI |
| MS.gene51850 | MS.gene95252 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51850.t1 | MTR_7g073700 | 76.107 | 745 | 93 | 5 | 1 | 681 | 188 | 911 | 0.0 | 1006 |
| MS.gene51850.t1 | MTR_7g073700 | 67.049 | 610 | 48 | 7 | 1 | 457 | 90 | 699 | 0.0 | 701 |
| MS.gene51850.t1 | MTR_0279s0010 | 57.746 | 213 | 38 | 3 | 99 | 259 | 12 | 224 | 6.41e-60 | 201 |
| MS.gene51850.t1 | MTR_7g073690 | 44.958 | 238 | 87 | 6 | 7 | 211 | 32 | 258 | 2.18e-38 | 151 |
| MS.gene51850.t1 | MTR_7g073690 | 61.972 | 71 | 27 | 0 | 141 | 211 | 24 | 94 | 2.34e-16 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51850.t1 | AT2G38580 | 34.249 | 473 | 234 | 10 | 207 | 674 | 81 | 481 | 8.98e-52 | 187 |
| MS.gene51850.t1 | AT2G38580 | 31.525 | 295 | 174 | 5 | 143 | 416 | 80 | 367 | 3.25e-20 | 94.7 |
| MS.gene51850.t1 | AT2G38580 | 34.505 | 455 | 209 | 11 | 230 | 674 | 85 | 460 | 4.09e-51 | 184 |
| MS.gene51850.t1 | AT2G38580 | 29.352 | 293 | 162 | 5 | 143 | 416 | 80 | 346 | 6.44e-16 | 81.3 |
| MS.gene51850.t1 | AT2G38580 | 33.453 | 278 | 139 | 5 | 207 | 481 | 81 | 315 | 9.29e-23 | 100 |
| MS.gene51850.t1 | AT2G38580 | 33.197 | 244 | 154 | 2 | 143 | 384 | 80 | 316 | 7.05e-20 | 92.0 |
| MS.gene51850.t1 | AT2G38580 | 30.000 | 280 | 145 | 4 | 169 | 446 | 85 | 315 | 7.88e-17 | 82.8 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51661 | gene | 922 | 7877 | 922 | ID=MS.gene51850 |
| 51661 | mRNA | 922 | 7877 | 922 | ID=MS.gene51850.t1;Parent=MS.gene51850 |
| 51661 | exon | 922 | 1287 | 922 | ID=MS.gene51850.t1.exon1;Parent=MS.gene51850.t1 |
| 51661 | CDS | 922 | 1287 | 922 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 1584 | 1649 | 1584 | ID=MS.gene51850.t1.exon2;Parent=MS.gene51850.t1 |
| 51661 | CDS | 1584 | 1649 | 1584 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 2291 | 2353 | 2291 | ID=MS.gene51850.t1.exon3;Parent=MS.gene51850.t1 |
| 51661 | CDS | 2291 | 2353 | 2291 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 2577 | 2639 | 2577 | ID=MS.gene51850.t1.exon4;Parent=MS.gene51850.t1 |
| 51661 | CDS | 2577 | 2639 | 2577 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 2740 | 2802 | 2740 | ID=MS.gene51850.t1.exon5;Parent=MS.gene51850.t1 |
| 51661 | CDS | 2740 | 2802 | 2740 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 2889 | 2952 | 2889 | ID=MS.gene51850.t1.exon6;Parent=MS.gene51850.t1 |
| 51661 | CDS | 2889 | 2952 | 2889 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 3199 | 3254 | 3199 | ID=MS.gene51850.t1.exon7;Parent=MS.gene51850.t1 |
| 51661 | CDS | 3199 | 3254 | 3199 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 3407 | 3469 | 3407 | ID=MS.gene51850.t1.exon8;Parent=MS.gene51850.t1 |
| 51661 | CDS | 3407 | 3469 | 3407 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 3658 | 3720 | 3658 | ID=MS.gene51850.t1.exon9;Parent=MS.gene51850.t1 |
| 51661 | CDS | 3658 | 3720 | 3658 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 3824 | 3865 | 3824 | ID=MS.gene51850.t1.exon10;Parent=MS.gene51850.t1 |
| 51661 | CDS | 3824 | 3865 | 3824 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 4036 | 4077 | 4036 | ID=MS.gene51850.t1.exon11;Parent=MS.gene51850.t1 |
| 51661 | CDS | 4036 | 4077 | 4036 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 4249 | 4311 | 4249 | ID=MS.gene51850.t1.exon12;Parent=MS.gene51850.t1 |
| 51661 | CDS | 4249 | 4311 | 4249 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 4601 | 4642 | 4601 | ID=MS.gene51850.t1.exon13;Parent=MS.gene51850.t1 |
| 51661 | CDS | 4601 | 4642 | 4601 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 4813 | 4875 | 4813 | ID=MS.gene51850.t1.exon14;Parent=MS.gene51850.t1 |
| 51661 | CDS | 4813 | 4875 | 4813 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 4987 | 5049 | 4987 | ID=MS.gene51850.t1.exon15;Parent=MS.gene51850.t1 |
| 51661 | CDS | 4987 | 5049 | 4987 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 5151 | 5213 | 5151 | ID=MS.gene51850.t1.exon16;Parent=MS.gene51850.t1 |
| 51661 | CDS | 5151 | 5213 | 5151 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 5366 | 5428 | 5366 | ID=MS.gene51850.t1.exon17;Parent=MS.gene51850.t1 |
| 51661 | CDS | 5366 | 5428 | 5366 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 6132 | 6191 | 6132 | ID=MS.gene51850.t1.exon18;Parent=MS.gene51850.t1 |
| 51661 | CDS | 6132 | 6191 | 6132 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 6728 | 6865 | 6728 | ID=MS.gene51850.t1.exon19;Parent=MS.gene51850.t1 |
| 51661 | CDS | 6728 | 6865 | 6728 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 7184 | 7282 | 7184 | ID=MS.gene51850.t1.exon20;Parent=MS.gene51850.t1 |
| 51661 | CDS | 7184 | 7282 | 7184 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| 51661 | exon | 7437 | 7877 | 7437 | ID=MS.gene51850.t1.exon21;Parent=MS.gene51850.t1 |
| 51661 | CDS | 7437 | 7877 | 7437 | ID=cds.MS.gene51850.t1;Parent=MS.gene51850.t1 |
| Gene Sequence |
| Protein sequence |
