AlfalfaGEDB | MS.gene70587

M. sativa cultivar XinJiangDaYe / MS.gene70587

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF13041.9 PF13812.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70587.t1	XP_013461055.1	96.5	490	14	1	1	487	1	490	6.10E-140	507.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70587.t1	Q9S7R4	75.6	464	106	4	30	487	20	482	7.2e-65	249.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70587.t1	A0A072UZ58	96.5	490	14	1	1	487	1	490	4.4e-140	507.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002806	MS.gene70587	PPI
MS.gene004491	MS.gene70587	PPI
MS.gene00454	MS.gene70587	PPI
MS.gene007439	MS.gene70587	PPI
MS.gene007508	MS.gene70587	PPI
MS.gene00961	MS.gene70587	PPI
MS.gene009807	MS.gene70587	PPI
MS.gene015792	MS.gene70587	PPI
MS.gene015797	MS.gene70587	PPI
MS.gene016665	MS.gene70587	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70587.t1	MTR_3g078580	96.122	490	16	1	1	487	1	490	0.0	972
MS.gene70587.t1	MTR_3g088505	31.163	430	291	3	34	463	34	458	1.82e-76	248
MS.gene70587.t1	MTR_8g071300	29.584	409	282	3	65	470	116	521	2.52e-63	217
MS.gene70587.t1	MTR_8g071300	23.457	243	181	3	238	479	150	388	2.33e-16	82.4
MS.gene70587.t1	MTR_5g006640	28.777	417	294	1	35	451	106	519	8.74e-61	208
MS.gene70587.t1	MTR_3g114700	30.548	347	240	1	79	425	125	470	4.03e-60	206
MS.gene70587.t1	MTR_3g114700	23.301	206	154	2	274	479	146	347	1.14e-13	73.6
MS.gene70587.t1	MTR_6g079830	28.818	347	243	2	104	449	232	575	6.08e-58	203
MS.gene70587.t1	MTR_6g079830	27.966	354	249	3	108	459	96	445	4.07e-45	167
MS.gene70587.t1	MTR_6g079830	24.481	241	148	1	99	339	367	573	1.31e-19	92.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70587.t1	AT1G74900	62.500	464	138	5	30	487	20	453	0.0	599
MS.gene70587.t1	AT1G77360	28.602	472	318	9	12	470	4	469	1.17e-65	219
MS.gene70587.t1	AT1G77360	28.602	472	318	9	12	470	40	505	4.13e-65	219
MS.gene70587.t1	AT5G65820	28.543	501	276	6	47	471	98	592	2.36e-61	212
MS.gene70587.t1	AT1G62680	31.445	353	238	2	112	463	135	484	1.73e-58	202
MS.gene70587.t1	AT1G62680	25.281	356	254	2	93	447	186	530	1.28e-38	148
MS.gene70587.t1	AT1G62680	24.615	325	241	2	147	470	65	386	1.76e-31	127
MS.gene70587.t1	AT1G62680	27.500	280	200	1	202	481	86	362	7.86e-30	122
MS.gene70587.t1	AT1G62680	31.445	353	238	2	112	463	135	484	1.73e-58	202
MS.gene70587.t1	AT1G62680	25.281	356	254	2	93	447	186	530	1.28e-38	148

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Find 149 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACCGCTTGGGCCTTAAT+GGG	0.458308	3.1:-64382090	MS.gene70587:CDS
AACCGGTGGAAAAGCTCATA+AGG	0.524360	3.1:-64382001	MS.gene70587:CDS
AAGTGGGGTCCATTGGAAAG+TGG	0.533486	3.1:+64382260	None:intergenic
AAGTGGGGTTTGATAGAGTT+TGG	0.283782	3.1:+64382277	None:intergenic
AAGTGGTGGATATTCGGGTA+CGG	0.634926	3.1:+64382356	None:intergenic
AAGTTGTTGATTGAAATGGT+TGG	0.680759	3.1:-64381127	MS.gene70587:CDS
AATATTATAAGTATCCAAGT+TGG	0.505964	3.1:+64381204	None:intergenic
AATGTTGTCGACGAGAAGTG+GGG	0.674549	3.1:+64382245	None:intergenic
AATTTGTTTAAAACTCTTAG+AGG	0.370908	3.1:-64381868	MS.gene70587:CDS
ACAACATTCTCAAACGCCTC+TGG	0.461582	3.1:-64382230	MS.gene70587:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACGTTATTATTGTGATGAAG+GGG	-	chr3.1:64382136-64382155	MS.gene70587:CDS	30.0%
ACTTATACTACTATGGTTCA+TGG	-	chr3.1:64381804-64381823	MS.gene70587:CDS	30.0%
AGATATTAAGAATGCAAAGC+AGG	-	chr3.1:64382396-64382415	MS.gene70587:CDS	30.0%
ATAATATAATGGCGAATGGT+TGG	-	chr3.1:64381598-64381617	MS.gene70587:CDS	30.0%
ATGAAAAGAATGGAGGAAAA+TGG	-	chr3.1:64382077-64382096	MS.gene70587:CDS	30.0%
CCCGATTAAAAGTAAACTTA+CGG	+	chr3.1:64382331-64382350	None:intergenic	30.0%
CCGATTAAAAGTAAACTTAC+GGG	+	chr3.1:64382330-64382349	None:intergenic	30.0%
CGATTAAAAGTAAACTTACG+GGG	+	chr3.1:64382329-64382348	None:intergenic	30.0%
TGAAAAGAATGGAGGAAAAT+GGG	-	chr3.1:64382078-64382097	MS.gene70587:CDS	30.0%
TGTGGAGTTTATGAAAAGAA+TGG	-	chr3.1:64382067-64382086	MS.gene70587:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	64380988	64382451	64380988	ID=MS.gene70587
chr3.1	mRNA	64380988	64382451	64380988	ID=MS.gene70587.t1;Parent=MS.gene70587
chr3.1	exon	64380988	64382451	64380988	ID=MS.gene70587.t1.exon1;Parent=MS.gene70587.t1
chr3.1	CDS	64380988	64382451	64380988	ID=cds.MS.gene70587.t1;Parent=MS.gene70587.t1

Gene Sequence

>MS.gene70587

ATGTTGAACCAGTTACTGGTTAAACCAGCTCAACGAACCTTGAACACTTCCCGATTCTGTCTTCCCTTCACCACCGTACCCGAATATCCACCACTTTCCATCGTCGCCACCGTCGCAAAACTCGTCCTCGAATCAGATCCAAAAACCCTCTCCCAAACTCTATCAAACCCCACTTTCCAATGGACCCCACTTCTCGTCGACAACATTCTCAAACGCCTCTGGAACCACGGACCCAAAGCCTTGCAATTCTTCAAACACCTAGACCGCCACCCAACCTACATCCACTCCACCTCCTCCTTCGAGCACGCCGTCGACATCGCCGCCCGGTTACGCGAATACAACACCGCTTGGGCCTTAATGGGCCGCATGCGGGCCCTCCGTCTCGGCCCAACTCCCAAAACCTTTGCAATCCTCGCTGAACGCTATGCAACCGGTGGAAAAGCTCATAAGGCTGTGAAAGTGTTTTTGTCAATGCATGAACATGGTTGTCATCAGGATTTGAATTCGTTCAACACAATCCTTGATATTCTTTGCAAGACGAGGCGCGTTGAAATGGCGAATAATTTGTTTAAAACTCTTAGAGGGAGGTTTAAATGTGATGGTGTTAGTTATAATATAATGGCGAATGGTTGGTGTTTGATTAAGCGAACACCGATGGCGTTGCAGGTTTTGAAGGAGATGGTGGAGAGGGGGATTAATCCGACGATGGTTACGTATAATACATTGTTGAAAGGCTATTTTAGGTGTGGCCAATTGAATGAAGCTTGGGAGTTTTTTTTGGAGATGAAGAAGAGGAAGTGTGAGATTGATGTGGTTACTTATACTACTATGGTTCATGGATTTGGTGTTGCTGGTGAGGTTAAGAGATCGAAGAGGGTTTTCGATGCAATGGTGAAAGCAGGTTTGGTTCCTTCTGTTGCTACTTATAATGCTTTGATTCAGGTTTTGTGTAAGAAGGATAGTGTGCAGAATGCTTTGTTGGTTTTTGATGAGATGGTTGAGAAAGGGTGTGTGCCTAATTTGACTACTTATAATGTTGTTATTAGAGGTTTGTGTCATTCGGGTGAAATCGAGAGAGGTGTGGAGTTTATGAAAAGAATGGAGGAAAATGGGTGCAGGCCAAGTGTTCAGACATACAATGTTGTGATACGTTATTATTGTGATGAAGGGGAACTTGAAAAGGGTTTGGAGTTGTTTGAAAAGATGGGGAATGGGACTTGTTTGCCCAACTTGGATACTTATAATATTCTGATTAGTGCCATGTTTGTGAGGAAGAAATCAGAGGATTTGGTGTTGGCTGGGAAGTTGTTGATTGAAATGGTTGGTAGAGGTTTCTTGCCCCGTAAGTTTACTTTTAATCGGGTGTTGAATGGACTTGTTCTAACGGGGAATCGAGAATTTGCGAATGAGATATTAAGAATGCAAAGCAGGTCTGGCCGTGTTCTTCGTCATGTGAAATTGTGA

Protein sequence

>MS.gene70587.t1

MLNQLLVKPAQRTLNTSRFCLPFTTVPEYPPLSIVATVAKLVLESDPKTLSQTLSNPTFQWTPLLVDNILKRLWNHGPKALQFFKHLDRHPTYIHSTSSFEHAVDIAARLREYNTAWALMGRMRALRLGPTPKTFAILAERYATGGKAHKAVKVFLSMHEHGCHQDLNSFNTILDILCKTRRVEMANNLFKTLRGRFKCDGVSYNIMANGWCLIKRTPMALQVLKEMVERGINPTMVTYNTLLKGYFRCGQLNEAWEFFLEMKKRKCEIDVVTYTTMVHGFGVAGEVKRSKRVFDAMVKAGLVPSVATYNALIQVLCKKDSVQNALLVFDEMVEKGCVPNLTTYNVVIRGLCHSGEIERGVEFMKRMEENGCRPSVQTYNVVIRYYCDEGELEKGLELFEKMGNGTCLPNLDTYNILISAMFVRKKSEDLVLAGKLLIEMVGRGFLPRKFTFNRVLNGLVLTGNREFANEILRMQSRSGRVLRHVKL