AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056541

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056541.t1 MTR_8g071280 98.826 596 7 0 1 596 1 596 0.0 1229
MS.gene056541.t1 MTR_5g026930 76.667 600 134 3 1 596 1 598 0.0 964
MS.gene056541.t1 MTR_5g026930 76.647 501 111 3 1 497 1 499 0.0 804
MS.gene056541.t1 MTR_1g101370 48.983 541 265 8 57 588 79 617 0.0 547
MS.gene056541.t1 MTR_8g432680 49.817 546 256 11 52 588 94 630 0.0 540
MS.gene056541.t1 MTR_0019s0120 48.921 556 266 10 43 590 83 628 0.0 531
MS.gene056541.t1 MTR_7g076170 50.854 527 247 10 71 588 102 625 1.53e-180 525
MS.gene056541.t1 MTR_8g100075 45.455 550 284 10 58 595 82 627 1.90e-174 509
MS.gene056541.t1 MTR_8g055840 42.479 605 323 10 10 595 9 607 4.84e-169 494
MS.gene056541.t1 MTR_5g040360 44.746 552 292 8 45 590 57 601 1.13e-166 488
MS.gene056541.t1 MTR_4g098890 47.159 528 266 7 66 587 82 602 2.27e-165 512
MS.gene056541.t1 MTR_7g006060 45.719 549 284 9 45 582 20 565 2.58e-165 483
MS.gene056541.t1 MTR_3g040370 42.762 601 311 11 16 595 16 604 2.57e-163 479
MS.gene056541.t1 MTR_8g432620 41.241 548 294 8 52 588 70 600 2.14e-156 462
MS.gene056541.t1 MTR_7g076150 41.398 558 296 9 45 588 47 587 3.17e-155 458
MS.gene056541.t1 MTR_5g080720 43.199 544 284 11 53 585 108 637 6.11e-153 454
MS.gene056541.t1 MTR_0019s0120 49.134 462 218 9 43 497 83 534 2.31e-150 444
MS.gene056541.t1 MTR_3g068990 42.989 542 282 11 57 587 142 667 9.78e-148 442
MS.gene056541.t1 MTR_1g442840 37.621 622 332 10 11 591 12 618 5.80e-145 433
MS.gene056541.t1 MTR_1g075900 38.782 624 324 11 11 592 11 618 2.94e-144 431
MS.gene056541.t1 MTR_1g029730 44.275 524 261 14 79 587 151 658 3.01e-144 432
MS.gene056541.t1 MTR_1g069785 39.000 600 325 12 11 591 11 588 2.58e-143 428
MS.gene056541.t1 MTR_1g442840 42.032 502 270 6 101 591 14 505 5.58e-143 424
MS.gene056541.t1 MTR_5g021100 40.952 525 275 13 79 588 329 833 5.40e-137 419
MS.gene056541.t1 MTR_3g096480 41.856 528 270 12 79 588 268 776 8.28e-137 417
MS.gene056541.t1 MTR_8g076650 39.964 548 302 11 52 588 359 890 2.78e-136 419
MS.gene056541.t1 MTR_8g093880 39.469 565 302 14 44 588 262 806 8.51e-133 408
MS.gene056541.t1 MTR_5g080720 43.243 481 249 10 53 523 108 574 2.02e-132 399
MS.gene056541.t1 MTR_7g076150 43.601 461 232 8 139 588 1 444 5.80e-132 394
MS.gene056541.t1 MTR_5g083150 39.231 520 284 9 79 588 101 598 2.73e-130 395
MS.gene056541.t1 MTR_3g088790 40.571 525 282 11 79 588 233 742 7.73e-128 393
MS.gene056541.t1 MTR_1g051055 37.311 595 331 12 22 588 23 603 6.62e-126 384
MS.gene056541.t1 MTR_3g071530 40.000 520 276 11 79 588 153 646 8.87e-126 385
MS.gene056541.t1 MTR_1g022255 36.973 522 288 13 78 588 208 699 6.66e-107 337
MS.gene056541.t1 MTR_4g083030 32.914 556 315 12 59 585 75 601 2.88e-95 304
MS.gene056541.t1 MTR_3g074780 30.978 552 335 14 64 585 150 685 3.72e-93 301
MS.gene056541.t1 MTR_5g064080 31.365 542 320 13 73 585 149 667 1.13e-91 296
MS.gene056541.t1 MTR_1g022255 33.248 391 224 11 78 458 208 571 5.69e-60 210
MS.gene056541.t1 MTR_5g033760 45.455 66 36 0 531 596 41 106 2.19e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056541.t1 AT4G19120 73.782 595 145 3 7 596 10 598 0.0 947
MS.gene056541.t1 AT4G19120 73.782 595 145 3 7 596 10 598 0.0 947
MS.gene056541.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene056541.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene056541.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene056541.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene056541.t1 AT4G19120 74.194 496 117 3 7 497 10 499 0.0 790
MS.gene056541.t1 AT4G00750 44.901 608 302 13 18 595 25 629 1.38e-180 525
MS.gene056541.t1 AT4G10440 46.429 532 276 6 70 594 93 622 9.81e-178 517
MS.gene056541.t1 AT2G45750 47.566 534 266 9 67 588 84 615 1.41e-175 512
MS.gene056541.t1 AT1G33170 46.679 527 271 7 70 588 110 634 6.72e-175 510
MS.gene056541.t1 AT4G18030 43.212 604 315 10 10 592 11 607 1.48e-171 501
MS.gene056541.t1 AT1G26850 44.853 544 286 8 61 595 77 615 2.51e-165 485
MS.gene056541.t1 AT1G26850 44.853 544 286 8 61 595 77 615 2.51e-165 485
MS.gene056541.t1 AT4G00740 44.023 527 267 8 73 588 84 593 2.09e-162 477
MS.gene056541.t1 AT4G00740 44.023 527 267 8 73 588 84 593 2.09e-162 477
MS.gene056541.t1 AT2G43200 45.800 500 264 7 70 565 91 587 5.42e-155 458
MS.gene056541.t1 AT2G43200 45.800 500 264 7 70 565 91 587 5.42e-155 458
MS.gene056541.t1 AT2G39750 43.831 543 280 11 56 587 163 691 3.35e-152 454
MS.gene056541.t1 AT1G77260 41.331 571 304 14 28 587 105 655 1.61e-146 438
MS.gene056541.t1 AT3G23300 39.138 603 330 8 16 591 12 604 1.07e-142 427
MS.gene056541.t1 AT3G23300 39.138 603 330 8 16 591 12 604 1.07e-142 427
MS.gene056541.t1 AT5G06050 40.741 540 291 11 61 587 140 663 1.54e-142 429
MS.gene056541.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene056541.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene056541.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene056541.t1 AT4G14360 41.341 537 291 7 69 591 75 601 2.11e-139 418
MS.gene056541.t1 AT4G14360 41.341 537 291 7 69 591 75 601 2.11e-139 418
MS.gene056541.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene056541.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene056541.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene056541.t1 AT2G40280 39.358 592 307 13 21 596 34 589 4.67e-138 414
MS.gene056541.t1 AT3G56080 43.636 495 253 13 79 564 114 591 1.88e-137 413
MS.gene056541.t1 AT1G29470 40.835 551 289 14 56 588 226 757 8.07e-136 414
MS.gene056541.t1 AT1G29470 40.835 551 289 14 56 588 226 757 8.07e-136 414
MS.gene056541.t1 AT5G64030 40.755 530 279 13 79 591 308 819 3.77e-132 407
MS.gene056541.t1 AT5G14430 39.000 600 324 11 17 588 19 604 1.59e-131 398
MS.gene056541.t1 AT5G14430 38.833 600 325 11 17 588 19 604 3.48e-130 395
MS.gene056541.t1 AT3G10200 36.198 605 345 12 6 588 3 588 1.62e-129 392
MS.gene056541.t1 AT3G51070 40.304 526 281 11 79 588 381 889 4.46e-128 398
MS.gene056541.t1 AT1G26850 43.765 425 227 6 61 478 77 496 2.79e-124 376
MS.gene056541.t1 AT3G56080 44.053 454 228 13 79 523 114 550 1.65e-123 375
MS.gene056541.t1 AT5G04060 36.227 599 337 13 17 588 17 597 6.48e-123 375
MS.gene056541.t1 AT3G10200 36.823 554 311 11 6 539 3 537 1.15e-117 362
MS.gene056541.t1 AT3G51070 42.105 456 234 10 79 518 381 822 5.69e-115 362
MS.gene056541.t1 AT1G19430 37.143 525 284 15 78 588 230 722 3.11e-108 341
MS.gene056541.t1 AT1G19430 37.143 525 284 15 78 588 230 722 3.11e-108 341
MS.gene056541.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene056541.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene056541.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene056541.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene056541.t1 AT1G78240 30.699 544 330 12 73 585 149 676 1.46e-89 291
MS.gene056541.t1 AT1G78240 30.699 544 330 12 73 585 149 676 1.46e-89 291
MS.gene056541.t1 AT2G03480 31.272 566 315 15 55 585 64 590 1.15e-85 278
MS.gene056541.t1 AT2G03480 31.150 565 328 15 55 585 64 601 1.28e-85 279
MS.gene056541.t1 AT1G13860 33.333 465 257 13 154 587 2 444 2.01e-79 258
MS.gene056541.t1 AT1G19430 34.937 395 215 13 78 459 230 595 5.15e-64 221
MS.gene056541.t1 AT2G03480 28.446 341 221 6 55 380 64 396 1.60e-42 159

Find 158 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCGTCCTGGAGGATTT+TGG 0.151747 8.3:+31213016 MS.gene056541:CDS
CTTATTCCATGGACAGAATT+TGG 0.162644 8.3:+31212834 MS.gene056541:CDS
ATAGTCCCATCTTTCATTTC+TGG 0.167085 8.3:-31212218 None:intergenic
TTCTATATGGGTGTAATCTT+TGG 0.198314 8.3:+31211323 MS.gene056541:CDS
CTGCTCTTTGGCCATCTAAT+TGG 0.211320 8.3:-31211931 None:intergenic
ATGTGGACTTTCTTTCTATA+TGG 0.220950 8.3:+31211310 MS.gene056541:CDS
CGGTAAATATGTTGATTTAA+TGG 0.261309 8.3:+31212184 MS.gene056541:CDS
CAATCAACATTGGTTGAAAA+AGG 0.293240 8.3:+31212109 MS.gene056541:CDS
CCCATCAAATTCCTTCGATA+TGG 0.309158 8.3:+31212794 MS.gene056541:CDS
ACTTATTGACGATGATTTCT+TGG 0.312151 8.3:-31211399 None:intergenic
CAAATTCTGTCCATGGAATA+AGG 0.319015 8.3:-31212833 None:intergenic
ATGGATATGAATACGGAATA+TGG 0.323237 8.3:+31213516 MS.gene056541:CDS
ACATATTTACCGACACCGTT+CGG 0.328019 8.3:-31212173 None:intergenic
TGTGGACTTTCTTTCTATAT+GGG 0.328602 8.3:+31211311 MS.gene056541:CDS
CAACATTCATCTCTGCTCTT+TGG 0.332417 8.3:-31211943 None:intergenic
TTATTGTAGCAGTCATTATC+AGG 0.334016 8.3:-31213201 None:intergenic
GGAGAGAAATTCCTCTTTCC+TGG 0.334164 8.3:+31212134 MS.gene056541:CDS
TAGCTGGGGTGGTGATTTGC+TGG 0.334950 8.3:+31212650 MS.gene056541:CDS
CGTGGTATCCCTGCAATTCT+TGG 0.339517 8.3:+31212744 MS.gene056541:CDS
CTGAGGCTGTCATCACATTT+AGG 0.345457 8.3:-31213246 None:intergenic
CATATTTACCGACACCGTTC+GGG 0.351894 8.3:-31212172 None:intergenic
AGCTCAGGTTCAATTTGCTT+TGG 0.352726 8.3:+31212719 MS.gene056541:CDS
CTTATTGACGATGATTTCTT+GGG 0.358122 8.3:-31211398 None:intergenic
ATTCGAACGGCCATTGATAC+TGG 0.358461 8.3:+31212239 MS.gene056541:CDS
TTACAGAGATGGAAGAAGTA+TGG 0.359526 8.3:+31211817 MS.gene056541:intron
TAGTCCCATCTTTCATTTCT+GGG 0.364638 8.3:-31212217 None:intergenic
TATGACCTCCTTCATCTTGC+AGG 0.378268 8.3:+31213788 MS.gene056541:CDS
AGCCACCAGGTCGTAAAATT+CGG 0.398973 8.3:-31214165 None:intergenic
GGAGTATACCATGCTGAATC+TGG 0.400239 8.3:-31213270 None:intergenic
TAAACCATTGAGATTCGTTC+TGG 0.401421 8.3:-31213384 None:intergenic
GATCCAAAGTTTAAGAAAAT+AGG 0.401896 8.3:+31213318 MS.gene056541:CDS
TGAAATGAAGTATGTGTTGC+TGG 0.403874 8.3:+31214135 MS.gene056541:CDS
GCAAGGACACCAAGAATTGC+AGG 0.407630 8.3:-31212753 None:intergenic
CCTTGATTGATGATCCTGTA+TGG 0.411232 8.3:+31213553 MS.gene056541:CDS
ATCTTATCAGTCCCAAGCTC+GGG 0.413990 8.3:-31213483 None:intergenic
TGATAGTCAATACTGCATTC+AGG 0.414829 8.3:-31211449 None:intergenic
TGATACAATTCTATCGTTCT+CGG 0.415528 8.3:-31211348 None:intergenic
GAAGTATGTGTTGCTGGAAA+TGG 0.420396 8.3:+31214141 MS.gene056541:CDS
AGGACAAAACAAGTGCGAAT+TGG 0.432966 8.3:-31213291 None:intergenic
ATCCTATCGGTTCATAATGT+TGG 0.442728 8.3:+31211840 MS.gene056541:CDS
ACGGCCATTGATACTGGTTG+TGG 0.443198 8.3:+31212245 MS.gene056541:CDS
GCTGGATCGTGGAATTCTTA+CGG 0.446421 8.3:+31212668 MS.gene056541:CDS
CTCCATGTCACCTCCTTGCT+CGG 0.448306 8.3:-31214275 None:intergenic
TCAGGAATGTTCCATTTGAC+TGG 0.450903 8.3:+31212069 MS.gene056541:intron
TGTTTGTTAATCCAGTCAAA+TGG 0.459476 8.3:-31212080 None:intergenic
TGTCCATGGAATAAGGCATC+TGG 0.467386 8.3:-31212826 None:intergenic
GGTACCAGCTTCCAATTAGA+TGG 0.467802 8.3:+31211920 MS.gene056541:CDS
ACCCTTGGCAATAGTTGTAA+TGG 0.468668 8.3:-31214224 None:intergenic
AAGAAGTTGATCCCCGAGCT+TGG 0.469511 8.3:+31213471 MS.gene056541:CDS
TTGGTGTCCTTGCTACTCAC+AGG 0.473805 8.3:+31212763 MS.gene056541:CDS
CTGATCCCAGAAATGAAAGA+TGG 0.476089 8.3:+31212212 MS.gene056541:CDS
GTTTATGATCGAGGCCTCAT+TGG 0.482541 8.3:+31213621 MS.gene056541:CDS
AAATGTGATGGATATGAATA+CGG 0.485516 8.3:+31213509 MS.gene056541:CDS
TCGAAGGAATTTGATGGGAA+TGG 0.487215 8.3:-31212789 None:intergenic
AGAAGTTGATCCCCGAGCTT+GGG 0.487598 8.3:+31213472 MS.gene056541:CDS
ATCCTCCAGGACGAAGAATC+CGG 0.487693 8.3:-31213011 None:intergenic
TGGTGTAACATGCAACCGCT+CGG 0.491978 8.3:-31213364 None:intergenic
CCCTGTGGCAATCATATTTG+TGG 0.495560 8.3:+31211283 MS.gene056541:CDS
GATCTGCGCTCGTAGTTGAT+TGG 0.499029 8.3:-31213054 None:intergenic
GAAGAATAATGAAGGAAAAG+TGG 0.501394 8.3:+31211241 MS.gene056541:CDS
GCCATATCGAAGGAATTTGA+TGG 0.506739 8.3:-31212795 None:intergenic
TGAGCTTGGTGGTTATCTCT+TGG 0.511910 8.3:-31212702 None:intergenic
AGCTGGTACCCATCCGGAGG+AGG 0.516830 8.3:-31211907 None:intergenic
TATCTTATCAGTCCCAAGCT+CGG 0.517834 8.3:-31213484 None:intergenic
GGTGTAACATGCAACCGCTC+GGG 0.518809 8.3:-31213363 None:intergenic
TCATTGGAACGTTCCACGAC+TGG 0.519029 8.3:+31213637 MS.gene056541:CDS
TGAAGAGTCCTGCAAGATGA+AGG 0.520104 8.3:-31213796 None:intergenic
TTGGCCATCTAATTGGAAGC+TGG 0.520718 8.3:-31211924 None:intergenic
ATTGGAAGCTGGTACCCATC+CGG 0.522262 8.3:-31211913 None:intergenic
CAAATTGAACCTGAGCTTGG+TGG 0.522756 8.3:-31212713 None:intergenic
TACACCAGAACGAATCTCAA+TGG 0.522832 8.3:+31213380 MS.gene056541:CDS
GCTCCAGATGCCTTATTCCA+TGG 0.526600 8.3:+31212823 MS.gene056541:CDS
TGCTTAGTTCCTCCTCCGGA+TGG 0.527581 8.3:+31211898 MS.gene056541:CDS
AAATTCCTCTTTCCTGGTGG+AGG 0.529642 8.3:+31212140 MS.gene056541:CDS
AGAATGCTTAGTTCCTCCTC+CGG 0.532704 8.3:+31211894 MS.gene056541:CDS
ACCACATTCATAACCCATAC+AGG 0.533600 8.3:-31213567 None:intergenic
AAGCAAATTGAACCTGAGCT+TGG 0.534577 8.3:-31212716 None:intergenic
AAGAGATAACCACCAAGCTC+AGG 0.536366 8.3:+31212704 MS.gene056541:CDS
GAAAAGTGGTAGACAAGAGT+AGG 0.538081 8.3:+31211255 MS.gene056541:CDS
TAGTACCTCCACCAGGAAAG+AGG 0.538108 8.3:-31212145 None:intergenic
CACTCCATGCACTGATCCAA+GGG 0.540931 8.3:+31211478 MS.gene056541:CDS
CACCCCACAACCAGTATCAA+TGG 0.542219 8.3:-31212249 None:intergenic
TGATCCCAGAAATGAAAGAT+GGG 0.543321 8.3:+31212213 MS.gene056541:CDS
GTAGTACCAAATTCTGTCCA+TGG 0.544289 8.3:-31212840 None:intergenic
CGGCCATTGATACTGGTTGT+GGG 0.544765 8.3:+31212246 MS.gene056541:CDS
GCAGAGATGAATGTTGGTAC+AGG 0.545315 8.3:+31211950 MS.gene056541:CDS
GTTCAATTTGCTTTGGAGCG+TGG 0.546321 8.3:+31212726 MS.gene056541:CDS
TGATAAGATAAGAAATGTGA+TGG 0.548109 8.3:+31213497 MS.gene056541:CDS
TATTTCATAATTACCAGTCG+TGG 0.552553 8.3:-31213650 None:intergenic
TGCCATTACAACTATTGCCA+AGG 0.553183 8.3:+31214222 MS.gene056541:CDS
ATCATATTTGTGGTGCTATG+TGG 0.553513 8.3:+31211293 MS.gene056541:CDS
CTGCGCTCGTAGTTGATTGG+TGG 0.554325 8.3:-31213051 None:intergenic
ACCACAAATATGATTGCCAC+AGG 0.556765 8.3:-31211284 None:intergenic
TTAATATTCTTGAAATACAC+CGG 0.559268 8.3:+31212992 MS.gene056541:CDS
ACACTCCATGCACTGATCCA+AGG 0.560389 8.3:+31211477 MS.gene056541:CDS
ATACACCGGATTCTTCGTCC+TGG 0.561320 8.3:+31213006 MS.gene056541:CDS
TACCGAGCAAGGAGGTGACA+TGG 0.562605 8.3:+31214273 MS.gene056541:CDS
TCAAACGTGACGGCAGTAAG+TGG 0.562817 8.3:+31213427 MS.gene056541:CDS
AACTACGAGCGCAGATCGCG+TGG 0.563131 8.3:+31213060 MS.gene056541:CDS
GTCATCACATTTAGGTGGAT+AGG 0.564284 8.3:-31213238 None:intergenic
AGGACGATGAAGAATAATGA+AGG 0.564405 8.3:+31211233 None:intergenic
ACTATGTTCCCGAACGGTGT+CGG 0.567956 8.3:+31212164 MS.gene056541:CDS
TCTCGAAAATTGGAGGGCAA+TGG 0.568556 8.3:-31211867 None:intergenic
ATCAGGAGACTTCTGCCATA+CGG 0.568603 8.3:-31213184 None:intergenic
CTCCCTGTGGTTTATGATCG+AGG 0.568992 8.3:+31213612 MS.gene056541:CDS
ACTGCATTCAGGAAAACTGA+TGG 0.569719 8.3:-31211438 None:intergenic
ACATTCCCATCGCATACCCT+TGG 0.569792 8.3:-31214239 None:intergenic
AGGCCTCGATCATAAACCAC+AGG 0.572832 8.3:-31213615 None:intergenic
TCTTCACTGCAGAAAGCCAC+AGG 0.573575 8.3:+31213813 MS.gene056541:CDS
CCATACAGGATCATCAATCA+AGG 0.575427 8.3:-31213553 None:intergenic
GACAAGACCCAAAATCCTCC+AGG 0.575555 8.3:-31213024 None:intergenic
GCTTAGTTCCTCCTCCGGAT+GGG 0.577164 8.3:+31211899 MS.gene056541:CDS
CAAAGAGCAGAGATGAATGT+TGG 0.578527 8.3:+31211944 MS.gene056541:CDS
ACGAGCGCAGATCGCGTGGA+TGG 0.579590 8.3:+31213064 MS.gene056541:CDS
TCTTAAACTTTGGATCCGGA+AGG 0.579696 8.3:-31213311 None:intergenic
CCATATCGAAGGAATTTGAT+GGG 0.583652 8.3:-31212794 None:intergenic
GGTGGTGATTTGCTGGATCG+TGG 0.586018 8.3:+31212657 MS.gene056541:CDS
CGAATCTCAATGGTTTATCG+TGG 0.588439 8.3:+31213390 MS.gene056541:CDS
CACCGGATTCTTCGTCCTGG+AGG 0.589366 8.3:+31213009 MS.gene056541:CDS
CTTGATTGATGATCCTGTAT+GGG 0.589605 8.3:+31213554 MS.gene056541:CDS
GTCTTGCATCAATTTCTAAG+TGG 0.591080 8.3:+31213340 MS.gene056541:CDS
TATTGCCAAGGGTATGCGAT+GGG 0.603217 8.3:+31214234 MS.gene056541:CDS
CGGATGAGAGCATAGCCACC+AGG 0.604150 8.3:-31214178 None:intergenic
GAGAAATTCCTCTTTCCTGG+TGG 0.605663 8.3:+31212137 MS.gene056541:CDS
AACAGAAGTCCAATCAACAT+TGG 0.605779 8.3:+31212099 MS.gene056541:CDS
ATTACCCTTGGATCAGTGCA+TGG 0.610417 8.3:-31211482 None:intergenic
TTATTGACGATGATTTCTTG+GGG 0.610889 8.3:-31211397 None:intergenic
CTATTGCCAAGGGTATGCGA+TGG 0.619397 8.3:+31214233 MS.gene056541:CDS
GAATCTCAATGGTTTATCGT+GGG 0.620901 8.3:+31213391 MS.gene056541:CDS
TATTGACGATGATTTCTTGG+GGG 0.626617 8.3:-31211396 None:intergenic
ATGGAAGCCTGTGAGTAGCA+AGG 0.627736 8.3:-31212770 None:intergenic
GGAAGAAGTATGGATCCTAT+CGG 0.630329 8.3:+31211827 MS.gene056541:CDS
TCCTGTATGGGTTATGAATG+TGG 0.630835 8.3:+31213566 MS.gene056541:CDS
TGCTGTGAATACACTCCCTG+TGG 0.631567 8.3:+31213599 MS.gene056541:CDS
AGGCTGTCATCACATTTAGG+TGG 0.634724 8.3:-31213243 None:intergenic
GAAAGATGGGACTATTCGAA+CGG 0.640093 8.3:+31212226 MS.gene056541:CDS
GCCATTACAACTATTGCCAA+GGG 0.640948 8.3:+31214223 MS.gene056541:CDS
GCCTCAGACCAGATTCAGCA+TGG 0.641516 8.3:+31213262 MS.gene056541:CDS
GGAGGTACTATGTTCCCGAA+CGG 0.648294 8.3:+31212158 MS.gene056541:CDS
TCTTATCAGTCCCAAGCTCG+GGG 0.654043 8.3:-31213482 None:intergenic
GATAGTACTTTCAAACGTGA+CGG 0.658518 8.3:+31213417 MS.gene056541:CDS
ACCATGCTGAATCTGGTCTG+AGG 0.664749 8.3:-31213263 None:intergenic
CAAGGACACCAAGAATTGCA+GGG 0.666290 8.3:-31212752 None:intergenic
GGCCATTGATACTGGTTGTG+GGG 0.668748 8.3:+31212247 MS.gene056541:CDS
CGTACAGAAGATACCGAGCA+AGG 0.670500 8.3:+31214262 MS.gene056541:CDS
TTCCAACATTATGAACCGAT+AGG 0.674489 8.3:-31211842 None:intergenic
CAATTTCTAAGTGGCCCGAG+CGG 0.675590 8.3:+31213349 MS.gene056541:CDS
GATATGAATACGGAATATGG+AGG 0.676561 8.3:+31213519 MS.gene056541:CDS
TGAAGGAGGTCATATGTCCG+AGG 0.677170 8.3:-31213779 None:intergenic
AGTCGTGGAACGTTCCAATG+AGG 0.677842 8.3:-31213635 None:intergenic
GGAAGCTGGTACCCATCCGG+AGG 0.678508 8.3:-31211910 None:intergenic
GGAGCAATGAGCCATATCGA+AGG 0.684039 8.3:-31212805 None:intergenic
AGAGTCCTGCAAGATGAAGG+AGG 0.687154 8.3:-31213793 None:intergenic
GGCCTCGATCATAAACCACA+GGG 0.703174 8.3:-31213614 None:intergenic
CAAGAGTAGGATCGTCCCTG+TGG 0.703595 8.3:+31211268 MS.gene056541:CDS
GGGAACATAGTACCTCCACC+AGG 0.712101 8.3:-31212152 None:intergenic
CCACAAATATGATTGCCACA+GGG 0.719216 8.3:-31211283 None:intergenic
ACAGAAGATACCGAGCAAGG+AGG 0.748039 8.3:+31214265 MS.gene056541:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTGTTATTAAAACAA+TGG + chr8.3:31213668-31213687 MS.gene056541:intron 10.0%
!!! ATTTGGTTTAAATATATTTT+TGG + chr8.3:31213879-31213898 MS.gene056541:intron 10.0%
!! TAAAGATTAAAATGATTACA+AGG + chr8.3:31214026-31214045 MS.gene056541:intron 15.0%
!! TATTTGTACACATTAATATT+TGG + chr8.3:31213862-31213881 MS.gene056541:intron 15.0%
!!! AAATTTGTTGATATTGAAAT+TGG + chr8.3:31213961-31213980 MS.gene056541:intron 15.0%
!!! ATTTTAATCTTTACTTTTAG+GGG - chr8.3:31214019-31214038 None:intergenic 15.0%
!!! CATTTTAATCTTTACTTTTA+GGG - chr8.3:31214020-31214039 None:intergenic 15.0%
!!! CTGATATTAATTGATTTTTT+AGG - chr8.3:31212554-31212573 None:intergenic 15.0%
!!! TCATTTTAATCTTTACTTTT+AGG - chr8.3:31214021-31214040 None:intergenic 15.0%
!! CCAAGATAATTTATAAAAAC+AGG + chr8.3:31211664-31211683 MS.gene056541:intron 20.0%
!! GTACAAATAACAAAATTAGT+TGG - chr8.3:31212532-31212551 None:intergenic 20.0%
!! TTAATATTCTTGAAATACAC+CGG + chr8.3:31212992-31213011 MS.gene056541:CDS 20.0%
!!! CCTGTTTTTATAAATTATCT+TGG - chr8.3:31211667-31211686 None:intergenic 20.0%
!!! GATTTGATTTTTGTTATGAT+AGG + chr8.3:31213735-31213754 MS.gene056541:intron 20.0%
!!! TATAGTTAATTTTACAGAGA+TGG + chr8.3:31211806-31211825 MS.gene056541:intron 20.0%
!!! TTTATCAGTGTTTTTTATCA+CGG + chr8.3:31212293-31212312 MS.gene056541:intron 20.0%
! ATAAGTTGTTTATCCAATCA+GGG + chr8.3:31211777-31211796 MS.gene056541:intron 25.0%
! CAGGAAACTTAAAATTATGT+CGG - chr8.3:31212013-31212032 None:intergenic 25.0%
! GATCCAAAGTTTAAGAAAAT+AGG + chr8.3:31213318-31213337 MS.gene056541:CDS 25.0%
! TAATATGCAAGAAAAAGCTA+TGG + chr8.3:31214305-31214324 MS.gene056541:CDS 25.0%
! TATAAGTTGTTTATCCAATC+AGG + chr8.3:31211776-31211795 MS.gene056541:intron 25.0%
! TGATAAGATAAGAAATGTGA+TGG + chr8.3:31213497-31213516 MS.gene056541:CDS 25.0%
!! AAAGAGAATTGTAATTTGCA+AGG - chr8.3:31212424-31212443 None:intergenic 25.0%
!! ACAAAACTCTTACTCATTTT+AGG + chr8.3:31212623-31212642 MS.gene056541:intron 25.0%
!! AGACCTATTTTCTTAAACTT+TGG - chr8.3:31213324-31213343 None:intergenic 25.0%
!! ATTTTCTTAAACTTTGGATC+CGG - chr8.3:31213318-31213337 None:intergenic 25.0%
!! CAATATCAACAAATTTTGCA+GGG - chr8.3:31213957-31213976 None:intergenic 25.0%
!! TAAAATGAGTAAGAGTTTTG+TGG - chr8.3:31212624-31212643 None:intergenic 25.0%
!! TCAATATCAACAAATTTTGC+AGG - chr8.3:31213958-31213977 None:intergenic 25.0%
!! TTGCTGTTATATCTAATTTC+AGG + chr8.3:31212051-31212070 MS.gene056541:intron 25.0%
!!! AAATGTGATGGATATGAATA+CGG + chr8.3:31213509-31213528 MS.gene056541:CDS 25.0%
!!! CGGTAAATATGTTGATTTAA+TGG + chr8.3:31212184-31212203 MS.gene056541:CDS 25.0%
AAATTATCTTGGACAGCTTA+TGG - chr8.3:31211656-31211675 None:intergenic 30.0%
AACACTGATAAAAACACAAG+AGG - chr8.3:31212285-31212304 None:intergenic 30.0%
ACTTATTGACGATGATTTCT+TGG - chr8.3:31211402-31211421 None:intergenic 30.0%
ATGGATATGAATACGGAATA+TGG + chr8.3:31213516-31213535 MS.gene056541:CDS 30.0%
ATGTGGACTTTCTTTCTATA+TGG + chr8.3:31211310-31211329 MS.gene056541:CDS 30.0%
CACTATACTAATATTACCCT+TGG - chr8.3:31211497-31211516 None:intergenic 30.0%
CATTAGAATAAGCTGTTTGT+TGG + chr8.3:31213839-31213858 MS.gene056541:intron 30.0%
CTGGTAAATGTTGCATAAAA+AGG - chr8.3:31212899-31212918 None:intergenic 30.0%
CTTATTGACGATGATTTCTT+GGG - chr8.3:31211401-31211420 None:intergenic 30.0%
GAAGAATAATGAAGGAAAAG+TGG + chr8.3:31211241-31211260 MS.gene056541:CDS 30.0%
GAGCTAGAATATGATTGTTA+AGG - chr8.3:31212447-31212466 None:intergenic 30.0%
GATACTTGTTTCATAGTTAC+TGG + chr8.3:31214066-31214085 MS.gene056541:intron 30.0%
TAACTACTATTCAACAACAC+TGG + chr8.3:31212380-31212399 MS.gene056541:intron 30.0%
TATTTCATAATTACCAGTCG+TGG - chr8.3:31213653-31213672 None:intergenic 30.0%
TGATACAATTCTATCGTTCT+CGG - chr8.3:31211351-31211370 None:intergenic 30.0%
TGTGGACTTTCTTTCTATAT+GGG + chr8.3:31211311-31211330 MS.gene056541:CDS 30.0%
TTAATGATCATGTGTCTGTT+TGG + chr8.3:31211559-31211578 MS.gene056541:intron 30.0%
TTATTGACGATGATTTCTTG+GGG - chr8.3:31211400-31211419 None:intergenic 30.0%
TTATTGTAGCAGTCATTATC+AGG - chr8.3:31213204-31213223 None:intergenic 30.0%
TTCATAGTTACTGGTATTTG+CGG + chr8.3:31214075-31214094 MS.gene056541:intron 30.0%
TTCTATATGGGTGTAATCTT+TGG + chr8.3:31211323-31211342 MS.gene056541:CDS 30.0%
TTCTGACAATGATTGTTACT+TGG + chr8.3:31214106-31214125 MS.gene056541:intron 30.0%
! AAAGTATTTGAGAGAGCTTA+TGG + chr8.3:31211588-31211607 MS.gene056541:intron 30.0%
! AATATTGCACCTTTTGCTTT+TGG + chr8.3:31213910-31213929 MS.gene056541:intron 30.0%
! CAATCAACATTGGTTGAAAA+AGG + chr8.3:31212109-31212128 MS.gene056541:CDS 30.0%
! CATTCTTTTCTCTCGAAAAT+TGG - chr8.3:31211880-31211899 None:intergenic 30.0%
! TGTTTGTTAATCCAGTCAAA+TGG - chr8.3:31212083-31212102 None:intergenic 30.0%
!! GTTGCGTTTTAATATGTCAT+GGG + chr8.3:31211516-31211535 MS.gene056541:intron 30.0%
!! TGTTGCGTTTTAATATGTCA+TGG + chr8.3:31211515-31211534 MS.gene056541:intron 30.0%
!!! AACAACACTGGTTTTTATGT+AGG + chr8.3:31212392-31212411 MS.gene056541:intron 30.0%
!!! ACAACACTGGTTTTTATGTA+GGG + chr8.3:31212393-31212412 MS.gene056541:intron 30.0%
!!! CTTTTTCAACCAATGTTGAT+TGG - chr8.3:31212111-31212130 None:intergenic 30.0%
AACAGAAGTCCAATCAACAT+TGG + chr8.3:31212099-31212118 MS.gene056541:CDS 35.0%
AACTATACTTAGACCCTGAT+TGG - chr8.3:31211793-31211812 None:intergenic 35.0%
AGATATAACAGCAACCATGA+CGG - chr8.3:31212045-31212064 None:intergenic 35.0%
ATAGTCCCATCTTTCATTTC+TGG - chr8.3:31212221-31212240 None:intergenic 35.0%
CAAATTCTGTCCATGGAATA+AGG - chr8.3:31212836-31212855 None:intergenic 35.0%
CCATATCGAAGGAATTTGAT+GGG - chr8.3:31212797-31212816 None:intergenic 35.0%
CTTATTCCATGGACAGAATT+TGG + chr8.3:31212834-31212853 MS.gene056541:CDS 35.0%
CTTGTAAAGTTCATGATCAC+TGG - chr8.3:31212918-31212937 None:intergenic 35.0%
GAATCTCAATGGTTTATCGT+GGG + chr8.3:31213391-31213410 MS.gene056541:CDS 35.0%
GATAGTACTTTCAAACGTGA+CGG + chr8.3:31213417-31213436 MS.gene056541:CDS 35.0%
GATATGAATACGGAATATGG+AGG + chr8.3:31213519-31213538 MS.gene056541:CDS 35.0%
GTCTTGCATCAATTTCTAAG+TGG + chr8.3:31213340-31213359 MS.gene056541:CDS 35.0%
TAAACCATTGAGATTCGTTC+TGG - chr8.3:31213387-31213406 None:intergenic 35.0%
TAGTAGTTATAGCGATGCTA+TGG - chr8.3:31212369-31212388 None:intergenic 35.0%
TAGTCCCATCTTTCATTTCT+GGG - chr8.3:31212220-31212239 None:intergenic 35.0%
TATTGACGATGATTTCTTGG+GGG - chr8.3:31211399-31211418 None:intergenic 35.0%
TCTCGCGATAGATAAAAGAA+TGG + chr8.3:31212587-31212606 MS.gene056541:intron 35.0%
TGAAATGAAGTATGTGTTGC+TGG + chr8.3:31214135-31214154 MS.gene056541:CDS 35.0%
TGATAGTCAATACTGCATTC+AGG - chr8.3:31211452-31211471 None:intergenic 35.0%
TGATCCCAGAAATGAAAGAT+GGG + chr8.3:31212213-31212232 MS.gene056541:CDS 35.0%
TTACAGAGATGGAAGAAGTA+TGG + chr8.3:31211817-31211836 MS.gene056541:intron 35.0%
TTCCAACATTATGAACCGAT+AGG - chr8.3:31211845-31211864 None:intergenic 35.0%
! AAGCATTTTCGACATATCCT+CGG + chr8.3:31213762-31213781 MS.gene056541:CDS 35.0%
! CAACATTGGTTGAAAAAGGA+AGG + chr8.3:31212113-31212132 MS.gene056541:CDS 35.0%
! CTTGATTGATGATCCTGTAT+GGG + chr8.3:31213554-31213573 MS.gene056541:CDS 35.0%
! CTTTTCTCTCGAAAATTGGA+GGG - chr8.3:31211876-31211895 None:intergenic 35.0%
! TCTTTTCTCTCGAAAATTGG+AGG - chr8.3:31211877-31211896 None:intergenic 35.0%
!! ATCATATTTGTGGTGCTATG+TGG + chr8.3:31211293-31211312 MS.gene056541:CDS 35.0%
!! ATCCTATCGGTTCATAATGT+TGG + chr8.3:31211840-31211859 MS.gene056541:CDS 35.0%
AAGCAAATTGAACCTGAGCT+TGG - chr8.3:31212719-31212738 None:intergenic 40.0%
ACATATTTACCGACACCGTT+CGG - chr8.3:31212176-31212195 None:intergenic 40.0%
ACCACAAATATGATTGCCAC+AGG - chr8.3:31211287-31211306 None:intergenic 40.0%
ACCACATTCATAACCCATAC+AGG - chr8.3:31213570-31213589 None:intergenic 40.0%
ACCCTTGGCAATAGTTGTAA+TGG - chr8.3:31214227-31214246 None:intergenic 40.0%
ACTGCATTCAGGAAAACTGA+TGG - chr8.3:31211441-31211460 None:intergenic 40.0%
AGGACAAAACAAGTGCGAAT+TGG - chr8.3:31213294-31213313 None:intergenic 40.0%
ATGATTTCTTGGGGGAATCA+AGG - chr8.3:31211391-31211410 None:intergenic 40.0%
CAAAGAGCAGAGATGAATGT+TGG + chr8.3:31211944-31211963 MS.gene056541:CDS 40.0%
CAACAAGGACCAAAAGCAAA+AGG - chr8.3:31213922-31213941 None:intergenic 40.0%
CAACATTCATCTCTGCTCTT+TGG - chr8.3:31211946-31211965 None:intergenic 40.0%
CCAAAAAGTAACTAGACTCG+CGG - chr8.3:31214201-31214220 None:intergenic 40.0%
CCACAAATATGATTGCCACA+GGG - chr8.3:31211286-31211305 None:intergenic 40.0%
CCATACAGGATCATCAATCA+AGG - chr8.3:31213556-31213575 None:intergenic 40.0%
CCCATCAAATTCCTTCGATA+TGG + chr8.3:31212794-31212813 MS.gene056541:CDS 40.0%
CGAATCTCAATGGTTTATCG+TGG + chr8.3:31213390-31213409 MS.gene056541:CDS 40.0%
CTGATCCCAGAAATGAAAGA+TGG + chr8.3:31212212-31212231 MS.gene056541:CDS 40.0%
GAAAAGTGGTAGACAAGAGT+AGG + chr8.3:31211255-31211274 MS.gene056541:CDS 40.0%
GAAAGATGGGACTATTCGAA+CGG + chr8.3:31212226-31212245 MS.gene056541:CDS 40.0%
GCCATATCGAAGGAATTTGA+TGG - chr8.3:31212798-31212817 None:intergenic 40.0%
GCCATTACAACTATTGCCAA+GGG + chr8.3:31214223-31214242 MS.gene056541:CDS 40.0%
GCTTATTCTAATGTCACCTG+TGG - chr8.3:31213832-31213851 None:intergenic 40.0%
GGGACTAATACAAGACAACA+AGG - chr8.3:31213937-31213956 None:intergenic 40.0%
GTAGTACCAAATTCTGTCCA+TGG - chr8.3:31212843-31212862 None:intergenic 40.0%
GTCATCACATTTAGGTGGAT+AGG - chr8.3:31213241-31213260 None:intergenic 40.0%
TACACCAGAACGAATCTCAA+TGG + chr8.3:31213380-31213399 MS.gene056541:CDS 40.0%
TATCTTATCAGTCCCAAGCT+CGG - chr8.3:31213487-31213506 None:intergenic 40.0%
TCAAGAATATTAACTCCGCC+TGG - chr8.3:31212985-31213004 None:intergenic 40.0%
TCAGGAATGTTCCATTTGAC+TGG + chr8.3:31212069-31212088 MS.gene056541:intron 40.0%
TCGAAGGAATTTGATGGGAA+TGG - chr8.3:31212792-31212811 None:intergenic 40.0%
TCTTAAACTTTGGATCCGGA+AGG - chr8.3:31213314-31213333 None:intergenic 40.0%
TGATTTCTTGGGGGAATCAA+GGG - chr8.3:31211390-31211409 None:intergenic 40.0%
TGCCATTACAACTATTGCCA+AGG + chr8.3:31214222-31214241 MS.gene056541:CDS 40.0%
! AGCTCAGGTTCAATTTGCTT+TGG + chr8.3:31212719-31212738 MS.gene056541:CDS 40.0%
! CCTTGATTGATGATCCTGTA+TGG + chr8.3:31213553-31213572 MS.gene056541:CDS 40.0%
! GAAGTATGTGTTGCTGGAAA+TGG + chr8.3:31214141-31214160 MS.gene056541:CDS 40.0%
! GGAAGAAGTATGGATCCTAT+CGG + chr8.3:31211827-31211846 MS.gene056541:CDS 40.0%
! TCACACTGCAATCTTTTACC+AGG + chr8.3:31212964-31212983 MS.gene056541:intron 40.0%
! TCCTGTATGGGTTATGAATG+TGG + chr8.3:31213566-31213585 MS.gene056541:CDS 40.0%
!!! ACTCATTTTAGGTTGCTAGC+TGG + chr8.3:31212634-31212653 MS.gene056541:intron 40.0%
!!! CTCATTTTAGGTTGCTAGCT+GGG + chr8.3:31212635-31212654 MS.gene056541:intron 40.0%
!!! GTGTTTTTTATCACGGAGCA+TGG + chr8.3:31212300-31212319 MS.gene056541:intron 40.0%
!!! TCATTTTAGGTTGCTAGCTG+GGG + chr8.3:31212636-31212655 MS.gene056541:intron 40.0%
AAAAAGGCGACATTGCCGTA+TGG + chr8.3:31213169-31213188 MS.gene056541:CDS 45.0%
AAATTCCTCTTTCCTGGTGG+AGG + chr8.3:31212140-31212159 MS.gene056541:CDS 45.0%
AAGAGATAACCACCAAGCTC+AGG + chr8.3:31212704-31212723 MS.gene056541:CDS 45.0%
AGAATGCTTAGTTCCTCCTC+CGG + chr8.3:31211894-31211913 MS.gene056541:CDS 45.0%
AGCCACCAGGTCGTAAAATT+CGG - chr8.3:31214168-31214187 None:intergenic 45.0%
AGGCTGTCATCACATTTAGG+TGG - chr8.3:31213246-31213265 None:intergenic 45.0%
ATCAGGAGACTTCTGCCATA+CGG - chr8.3:31213187-31213206 None:intergenic 45.0%
ATCTTATCAGTCCCAAGCTC+GGG - chr8.3:31213486-31213505 None:intergenic 45.0%
ATTACCCTTGGATCAGTGCA+TGG - chr8.3:31211485-31211504 None:intergenic 45.0%
ATTCGAACGGCCATTGATAC+TGG + chr8.3:31212239-31212258 MS.gene056541:CDS 45.0%
CAAATTGAACCTGAGCTTGG+TGG - chr8.3:31212716-31212735 None:intergenic 45.0%
CAAGGACACCAAGAATTGCA+GGG - chr8.3:31212755-31212774 None:intergenic 45.0%
CATATTTACCGACACCGTTC+GGG - chr8.3:31212175-31212194 None:intergenic 45.0%
CCCTGTGGCAATCATATTTG+TGG + chr8.3:31211283-31211302 MS.gene056541:CDS 45.0%
CTGAGGCTGTCATCACATTT+AGG - chr8.3:31213249-31213268 None:intergenic 45.0%
CTGCTCTTTGGCCATCTAAT+TGG - chr8.3:31211934-31211953 None:intergenic 45.0%
GAGAAATTCCTCTTTCCTGG+TGG + chr8.3:31212137-31212156 MS.gene056541:CDS 45.0%
GCAGAGATGAATGTTGGTAC+AGG + chr8.3:31211950-31211969 MS.gene056541:CDS 45.0%
GCTGGATCGTGGAATTCTTA+CGG + chr8.3:31212668-31212687 MS.gene056541:CDS 45.0%
GGAGAGAAATTCCTCTTTCC+TGG + chr8.3:31212134-31212153 MS.gene056541:CDS 45.0%
GGAGTATACCATGCTGAATC+TGG - chr8.3:31213273-31213292 None:intergenic 45.0%
GGTACCAGCTTCCAATTAGA+TGG + chr8.3:31211920-31211939 MS.gene056541:CDS 45.0%
GTTTATGATCGAGGCCTCAT+TGG + chr8.3:31213621-31213640 MS.gene056541:CDS 45.0%
TATCACGGAGCATGGAATAG+CGG + chr8.3:31212308-31212327 MS.gene056541:intron 45.0%
TATGACCTCCTTCATCTTGC+AGG + chr8.3:31213788-31213807 MS.gene056541:CDS 45.0%
TCTCGAAAATTGGAGGGCAA+TGG - chr8.3:31211870-31211889 None:intergenic 45.0%
TGAAGAGTCCTGCAAGATGA+AGG - chr8.3:31213799-31213818 None:intergenic 45.0%
TTGGCCATCTAATTGGAAGC+TGG - chr8.3:31211927-31211946 None:intergenic 45.0%
! ATGGACCGAATTTTACGACC+TGG + chr8.3:31214160-31214179 MS.gene056541:CDS 45.0%
! CACTGCAATCTTTTACCAGG+CGG + chr8.3:31212967-31212986 MS.gene056541:intron 45.0%
! GGAATATGGAGGTTTTGCTG+CGG + chr8.3:31213530-31213549 MS.gene056541:CDS 45.0%
! TATTGCCAAGGGTATGCGAT+GGG + chr8.3:31214234-31214253 MS.gene056541:CDS 45.0%
! TCTTCGTCCTGGAGGATTTT+GGG + chr8.3:31213017-31213036 MS.gene056541:CDS 45.0%
! TGAGCTTGGTGGTTATCTCT+TGG - chr8.3:31212705-31212724 None:intergenic 45.0%
! TGTCCATGGAATAAGGCATC+TGG - chr8.3:31212829-31212848 None:intergenic 45.0%
! TTCTTCGTCCTGGAGGATTT+TGG + chr8.3:31213016-31213035 MS.gene056541:CDS 45.0%
!! GTTCAATTTGCTTTGGAGCG+TGG + chr8.3:31212726-31212745 MS.gene056541:CDS 45.0%
!!! CCGCGAGTCTAGTTACTTTT+TGG + chr8.3:31214198-31214217 MS.gene056541:CDS 45.0%
!!! TCGCACTTGTTTTGTCCTTC+CGG + chr8.3:31213296-31213315 MS.gene056541:CDS 45.0%
!! TTTAAATTGTATAATAAAAA+AGG + chr8.3:31213153-31213172 MS.gene056541:CDS 5.0%
ACAGAAGATACCGAGCAAGG+AGG + chr8.3:31214265-31214284 MS.gene056541:CDS 50.0%
ACATTCCCATCGCATACCCT+TGG - chr8.3:31214242-31214261 None:intergenic 50.0%
ACCATGCTGAATCTGGTCTG+AGG - chr8.3:31213266-31213285 None:intergenic 50.0%
ACTATGTTCCCGAACGGTGT+CGG + chr8.3:31212164-31212183 MS.gene056541:CDS 50.0%
AGAGTCCTGCAAGATGAAGG+AGG - chr8.3:31213796-31213815 None:intergenic 50.0%
AGGCCTCGATCATAAACCAC+AGG - chr8.3:31213618-31213637 None:intergenic 50.0%
AGTCGTGGAACGTTCCAATG+AGG - chr8.3:31213638-31213657 None:intergenic 50.0%
ATACACCGGATTCTTCGTCC+TGG + chr8.3:31213006-31213025 MS.gene056541:CDS 50.0%
ATCCTCCAGGACGAAGAATC+CGG - chr8.3:31213014-31213033 None:intergenic 50.0%
ATGGAAGCCTGTGAGTAGCA+AGG - chr8.3:31212773-31212792 None:intergenic 50.0%
ATTGGAAGCTGGTACCCATC+CGG - chr8.3:31211916-31211935 None:intergenic 50.0%
CAATTTCTAAGTGGCCCGAG+CGG + chr8.3:31213349-31213368 MS.gene056541:CDS 50.0%
CACCCCACAACCAGTATCAA+TGG - chr8.3:31212252-31212271 None:intergenic 50.0%
CGTACAGAAGATACCGAGCA+AGG + chr8.3:31214262-31214281 MS.gene056541:CDS 50.0%
CGTGGTATCCCTGCAATTCT+TGG + chr8.3:31212744-31212763 MS.gene056541:CDS 50.0%
CTCCCTGTGGTTTATGATCG+AGG + chr8.3:31213612-31213631 MS.gene056541:CDS 50.0%
GACAAGACCCAAAATCCTCC+AGG - chr8.3:31213027-31213046 None:intergenic 50.0%
GATCTGCGCTCGTAGTTGAT+TGG - chr8.3:31213057-31213076 None:intergenic 50.0%
GCAAGGACACCAAGAATTGC+AGG - chr8.3:31212756-31212775 None:intergenic 50.0%
GGAGCAATGAGCCATATCGA+AGG - chr8.3:31212808-31212827 None:intergenic 50.0%
GGCCTCGATCATAAACCACA+GGG - chr8.3:31213617-31213636 None:intergenic 50.0%
TAGTACCTCCACCAGGAAAG+AGG - chr8.3:31212148-31212167 None:intergenic 50.0%
TCAAACGTGACGGCAGTAAG+TGG + chr8.3:31213427-31213446 MS.gene056541:CDS 50.0%
TCATTGGAACGTTCCACGAC+TGG + chr8.3:31213637-31213656 MS.gene056541:CDS 50.0%
TCCTGCAACTTACTCCGTCA+TGG + chr8.3:31212028-31212047 MS.gene056541:intron 50.0%
TCTTATCAGTCCCAAGCTCG+GGG - chr8.3:31213485-31213504 None:intergenic 50.0%
TCTTCACTGCAGAAAGCCAC+AGG + chr8.3:31213813-31213832 MS.gene056541:CDS 50.0%
TGAAGGAGGTCATATGTCCG+AGG - chr8.3:31213782-31213801 None:intergenic 50.0%
TGCTGTGAATACACTCCCTG+TGG + chr8.3:31213599-31213618 MS.gene056541:CDS 50.0%
TGGTGTAACATGCAACCGCT+CGG - chr8.3:31213367-31213386 None:intergenic 50.0%
! AAGAAGTTGATCCCCGAGCT+TGG + chr8.3:31213471-31213490 MS.gene056541:CDS 50.0%
! ACACTCCATGCACTGATCCA+AGG + chr8.3:31211477-31211496 MS.gene056541:CDS 50.0%
! ACCATGACGGAGTAAGTTGC+AGG - chr8.3:31212032-31212051 None:intergenic 50.0%
! ACGGCCATTGATACTGGTTG+TGG + chr8.3:31212245-31212264 MS.gene056541:CDS 50.0%
! AGAAGTTGATCCCCGAGCTT+GGG + chr8.3:31213472-31213491 MS.gene056541:CDS 50.0%
! CACTCCATGCACTGATCCAA+GGG + chr8.3:31211478-31211497 MS.gene056541:CDS 50.0%
! CGGCCATTGATACTGGTTGT+GGG + chr8.3:31212246-31212265 MS.gene056541:CDS 50.0%
! CTATTGCCAAGGGTATGCGA+TGG + chr8.3:31214233-31214252 MS.gene056541:CDS 50.0%
! GACCGAATTTTACGACCTGG+TGG + chr8.3:31214163-31214182 MS.gene056541:CDS 50.0%
! GCTCCAGATGCCTTATTCCA+TGG + chr8.3:31212823-31212842 MS.gene056541:CDS 50.0%
! GGCCATTGATACTGGTTGTG+GGG + chr8.3:31212247-31212266 MS.gene056541:CDS 50.0%
! TTGGTGTCCTTGCTACTCAC+AGG + chr8.3:31212763-31212782 MS.gene056541:CDS 50.0%
!! GGAGGATTTTGGGTCTTGTC+TGG + chr8.3:31213027-31213046 MS.gene056541:CDS 50.0%
!! GGAGGTACTATGTTCCCGAA+CGG + chr8.3:31212158-31212177 MS.gene056541:CDS 50.0%
!!! TTTTAGGTTGCTAGCTGGGG+TGG + chr8.3:31212639-31212658 MS.gene056541:intron 50.0%
CAAGAGTAGGATCGTCCCTG+TGG + chr8.3:31211268-31211287 MS.gene056541:CDS 55.0%
CTCCATGTCACCTCCTTGCT+CGG - chr8.3:31214278-31214297 None:intergenic 55.0%
CTGCGCTCGTAGTTGATTGG+TGG - chr8.3:31213054-31213073 None:intergenic 55.0%
GCCTCAGACCAGATTCAGCA+TGG + chr8.3:31213262-31213281 MS.gene056541:CDS 55.0%
GCTTAGTTCCTCCTCCGGAT+GGG + chr8.3:31211899-31211918 MS.gene056541:CDS 55.0%
GGGAACATAGTACCTCCACC+AGG - chr8.3:31212155-31212174 None:intergenic 55.0%
GGTGTAACATGCAACCGCTC+GGG - chr8.3:31213366-31213385 None:intergenic 55.0%
TACCGAGCAAGGAGGTGACA+TGG + chr8.3:31214273-31214292 MS.gene056541:CDS 55.0%
TGCTTAGTTCCTCCTCCGGA+TGG + chr8.3:31211898-31211917 MS.gene056541:CDS 55.0%
TGGGGGAATCAAGGGATCTC+TGG - chr8.3:31211382-31211401 None:intergenic 55.0%
! TAGCTGGGGTGGTGATTTGC+TGG + chr8.3:31212650-31212669 MS.gene056541:CDS 55.0%
!! GGTGGTGATTTGCTGGATCG+TGG + chr8.3:31212657-31212676 MS.gene056541:CDS 55.0%
AACTACGAGCGCAGATCGCG+TGG + chr8.3:31213060-31213079 MS.gene056541:CDS 60.0%
CACCGGATTCTTCGTCCTGG+AGG + chr8.3:31213009-31213028 MS.gene056541:CDS 60.0%
! CGGATGAGAGCATAGCCACC+AGG - chr8.3:31214181-31214200 None:intergenic 60.0%
ACGAGCGCAGATCGCGTGGA+TGG + chr8.3:31213064-31213083 MS.gene056541:CDS 65.0%
AGCTGGTACCCATCCGGAGG+AGG - chr8.3:31211910-31211929 None:intergenic 65.0%
GGAAGCTGGTACCCATCCGG+AGG - chr8.3:31211913-31211932 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 31211239 31214354 31211239 ID=MS.gene056541
chr8.3 mRNA 31211239 31214354 31211239 ID=MS.gene056541.t1;Parent=MS.gene056541
chr8.3 exon 31211239 31211499 31211239 ID=MS.gene056541.t1.exon1;Parent=MS.gene056541.t1
chr8.3 CDS 31211239 31211499 31211239 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31211823 31211971 31211823 ID=MS.gene056541.t1.exon2;Parent=MS.gene056541.t1
chr8.3 CDS 31211823 31211971 31211823 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31212073 31212268 31212073 ID=MS.gene056541.t1.exon3;Parent=MS.gene056541.t1
chr8.3 CDS 31212073 31212268 31212073 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31212645 31212855 31212645 ID=MS.gene056541.t1.exon4;Parent=MS.gene056541.t1
chr8.3 CDS 31212645 31212855 31212645 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31212986 31213658 31212986 ID=MS.gene056541.t1.exon5;Parent=MS.gene056541.t1
chr8.3 CDS 31212986 31213658 31212986 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31213757 31213834 31213757 ID=MS.gene056541.t1.exon6;Parent=MS.gene056541.t1
chr8.3 CDS 31213757 31213834 31213757 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
chr8.3 exon 31214132 31214354 31214132 ID=MS.gene056541.t1.exon7;Parent=MS.gene056541.t1
chr8.3 CDS 31214132 31214354 31214132 ID=cds.MS.gene056541.t1;Parent=MS.gene056541.t1
Gene Sequence

>MS.gene056541

ATGAAGAATAATGAAGGAAAAGTGGTAGACAAGAGTAGGATCGTCCCTGTGGCAATCATATTTGTGGTGCTATGTGGACTTTCTTTCTATATGGGTGTAATCTTTGGTTCCGAGAACGATAGAATTGTATCAATTATCAGCCAGAGATCCCTTGATTCCCCCAAGAAATCATCGTCAATAAGTTCTCTGCAAATTAAATCCATCAGTTTTCCTGAATGCAGTATTGACTATCAAGATTACACTCCATGCACTGATCCAAGGGTAATATTAGTATAGTGTTGCGTTTTAATATGTCATGGGATTTGTAGAAGAAAGTTTTCTTAATGATCATGTGTCTGTTTGGATCGTAAAAGTATTTGAGAGAGCTTATGGAAACAACTTATGATATGTCTATAAGTTGTTTTCAGTTTATTTCCATAAGCTGTCCAAGATAATTTATAAAAACAGGCTATATCTTGTATAAAAACAATTTGTCTTTATTTTATCTTTTATTATAGATATAGCTTATTCACAATCATTTACAATAAGCTCTTATGCTATAAGTTGTTTATCCAATCAGGGTCTAAGTATAGTTAATTTTACAGAGATGGAAGAAGTATGGATCCTATCGGTTCATAATGTTGGAACGCCATTGCCCTCCAATTTTCGAGAGAAAAGAATGCTTAGTTCCTCCTCCGGATGGGTACCAGCTTCCAATTAGATGGCCAAAGAGCAGAGATGAATGTTGGTACAGGTAATTATATAAGTCTGATGACGAATGATAATATAAGACCGACATAATTTTAAGTTTCCTGCAACTTACTCCGTCATGGTTGCTGTTATATCTAATTTCAGGAATGTTCCATTTGACTGGATTAACAAACAGAAGTCCAATCAACATTGGTTGAAAAAGGAAGGAGAGAAATTCCTCTTTCCTGGTGGAGGTACTATGTTCCCGAACGGTGTCGGTAAATATGTTGATTTAATGGAAGATCTGATCCCAGAAATGAAAGATGGGACTATTCGAACGGCCATTGATACTGGTTGTGGGGTGAGTCTTGCTTCCTCTTGTGTTTTTATCAGTGTTTTTTATCACGGAGCATGGAATAGCGGTATGCTCAAATTCTGCTACACTACAGTCTACAGCGCCATAGCATCGCTATAACTACTATTCAACAACACTGGTTTTTATGTAGGGAATAGCCTTGCAAATTACAATTCTCTTTCCTTAACAATCATATTCTAGCTCAATTATATGTGCTTCTTCTTGCAGTTGTGCTTTTCTAACTACATTGTAAATATATTTTAAAACCAACTAATTTTGTTATTTGTACCTAAAAAATCAATTAATATCAGTTGCAATTTTCAATCTCGCGATAGATAAAAGAATGGCATCAATTAATCCACAAAACTCTTACTCATTTTAGGTTGCTAGCTGGGGTGGTGATTTGCTGGATCGTGGAATTCTTACGGTTTCTCTTGCACCAAGAGATAACCACCAAGCTCAGGTTCAATTTGCTTTGGAGCGTGGTATCCCTGCAATTCTTGGTGTCCTTGCTACTCACAGGCTTCCATTCCCATCAAATTCCTTCGATATGGCTCATTGCTCCAGATGCCTTATTCCATGGACAGAATTTGGTACTACTATGAATTTTGCTCTTAATCAAACTGAATTAATCCTTTTTATGCAACATTTACCAGTGATCATGAACTTTACAAGTCTTGTAGCTTCTTAAACTTTAAAATTCACACTGCAATCTTTTACCAGGCGGAGTTAATATTCTTGAAATACACCGGATTCTTCGTCCTGGAGGATTTTGGGTCTTGTCTGGTCCACCAATCAACTACGAGCGCAGATCGCGTGGATGGAACACAACTATCGAAGAGCAAAGATCAACTTACGAGAAATTGCAGAACTTACTGACTTCATTGTGCTTTAAATTGTATAATAAAAAAGGCGACATTGCCGTATGGCAGAAGTCTCCTGATAATGACTGCTACAATAAGCTGACTAGAGATACCTATCCACCTAAATGTGATGACAGCCTCAGACCAGATTCAGCATGGTATACTCCAATTCGCACTTGTTTTGTCCTTCCGGATCCAAAGTTTAAGAAAATAGGTCTTGCATCAATTTCTAAGTGGCCCGAGCGGTTGCATGTTACACCAGAACGAATCTCAATGGTTTATCGTGGGAGTGATAGTACTTTCAAACGTGACGGCAGTAAGTGGAAGAAGCATGTCGCACACTACAAGAAGTTGATCCCCGAGCTTGGGACTGATAAGATAAGAAATGTGATGGATATGAATACGGAATATGGAGGTTTTGCTGCGGCCTTGATTGATGATCCTGTATGGGTTATGAATGTGGTCTCATCTTATGCTGTGAATACACTCCCTGTGGTTTATGATCGAGGCCTCATTGGAACGTTCCACGACTGGTAATTATGAAATATTGTTATTAAAACAATGGTTTTATTTCTGTATATAATTTTATCTTATAAATAATCTACTGTTGATTTGATTTTTGTTATGATAGGTGTGAAGCATTTTCGACATATCCTCGGACATATGACCTCCTTCATCTTGCAGGACTCTTCACTGCAGAAAGCCACAGGTGACATTAGAATAAGCTGTTTGTTGGTATTTGTACACATTAATATTTGGTTTAAATATATTTTTGGTCTTTGCAAATATTGCACCTTTTGCTTTTGGTCCTTGTTGTCTTGTATTAGTCCCTGCAAAATTTGTTGATATTGAAATTGGTGTTTGTAATATGTAAAATATTTGATTTTAGTCCCCTAAAAGTAAAGATTAAAATGATTACAAGGCTAATTTGATAAGTGATGATACTTGTTTCATAGTTACTGGTATTTGCGGCTGTTTCATTCTGACAATGATTGTTACTTGGCAGATGTGAAATGAAGTATGTGTTGCTGGAAATGGACCGAATTTTACGACCTGGTGGCTATGCTCTCATCCGCGAGTCTAGTTACTTTTTGGATGCCATTACAACTATTGCCAAGGGTATGCGATGGGAATGTCGTACAGAAGATACCGAGCAAGGAGGTGACATGGAGAAGATACTAATATGCAAGAAAAAGCTATGGTATTCATCAAATAAAGATTCAAGATGA
Protein sequence

>MS.gene056541.t1

MKNNEGKVVDKSRIVPVAIIFVVLCGLSFYMGVIFGSENDRIVSIISQRSLDSPKKSSSISSLQIKSISFPECSIDYQDYTPCTDPRRWKKYGSYRFIMLERHCPPIFERKECLVPPPDGYQLPIRWPKSRDECWYRNVPFDWINKQKSNQHWLKKEGEKFLFPGGGTMFPNGVGKYVDLMEDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHQAQVQFALERGIPAILGVLATHRLPFPSNSFDMAHCSRCLIPWTEFGGVNILEIHRILRPGGFWVLSGPPINYERRSRGWNTTIEEQRSTYEKLQNLLTSLCFKLYNKKGDIAVWQKSPDNDCYNKLTRDTYPPKCDDSLRPDSAWYTPIRTCFVLPDPKFKKIGLASISKWPERLHVTPERISMVYRGSDSTFKRDGSKWKKHVAHYKKLIPELGTDKIRNVMDMNTEYGGFAAALIDDPVWVMNVVSSYAVNTLPVVYDRGLIGTFHDWCEAFSTYPRTYDLLHLAGLFTAESHRCEMKYVLLEMDRILRPGGYALIRESSYFLDAITTIAKGMRWECRTEDTEQGGDMEKILICKKKLWYSSNKDSR