AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067899

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067899.t1 MTR_8g071280 98.826 596 7 0 1 596 1 596 0.0 1229
MS.gene067899.t1 MTR_5g026930 76.667 600 134 3 1 596 1 598 0.0 964
MS.gene067899.t1 MTR_5g026930 76.647 501 111 3 1 497 1 499 0.0 804
MS.gene067899.t1 MTR_1g101370 48.983 541 265 8 57 588 79 617 0.0 547
MS.gene067899.t1 MTR_8g432680 49.817 546 256 11 52 588 94 630 0.0 540
MS.gene067899.t1 MTR_0019s0120 48.921 556 266 10 43 590 83 628 0.0 531
MS.gene067899.t1 MTR_7g076170 50.854 527 247 10 71 588 102 625 1.53e-180 525
MS.gene067899.t1 MTR_8g100075 45.455 550 284 10 58 595 82 627 1.90e-174 509
MS.gene067899.t1 MTR_8g055840 42.479 605 323 10 10 595 9 607 4.84e-169 494
MS.gene067899.t1 MTR_5g040360 44.746 552 292 8 45 590 57 601 1.13e-166 488
MS.gene067899.t1 MTR_4g098890 47.159 528 266 7 66 587 82 602 2.27e-165 512
MS.gene067899.t1 MTR_7g006060 45.719 549 284 9 45 582 20 565 2.58e-165 483
MS.gene067899.t1 MTR_3g040370 42.762 601 311 11 16 595 16 604 2.57e-163 479
MS.gene067899.t1 MTR_8g432620 41.241 548 294 8 52 588 70 600 2.14e-156 462
MS.gene067899.t1 MTR_7g076150 41.398 558 296 9 45 588 47 587 3.17e-155 458
MS.gene067899.t1 MTR_5g080720 43.199 544 284 11 53 585 108 637 6.11e-153 454
MS.gene067899.t1 MTR_0019s0120 49.134 462 218 9 43 497 83 534 2.31e-150 444
MS.gene067899.t1 MTR_3g068990 42.989 542 282 11 57 587 142 667 9.78e-148 442
MS.gene067899.t1 MTR_1g442840 37.621 622 332 10 11 591 12 618 5.80e-145 433
MS.gene067899.t1 MTR_1g075900 38.782 624 324 11 11 592 11 618 2.94e-144 431
MS.gene067899.t1 MTR_1g029730 44.275 524 261 14 79 587 151 658 3.01e-144 432
MS.gene067899.t1 MTR_1g069785 39.000 600 325 12 11 591 11 588 2.58e-143 428
MS.gene067899.t1 MTR_1g442840 42.032 502 270 6 101 591 14 505 5.58e-143 424
MS.gene067899.t1 MTR_5g021100 40.952 525 275 13 79 588 329 833 5.40e-137 419
MS.gene067899.t1 MTR_3g096480 41.856 528 270 12 79 588 268 776 8.28e-137 417
MS.gene067899.t1 MTR_8g076650 39.964 548 302 11 52 588 359 890 2.78e-136 419
MS.gene067899.t1 MTR_8g093880 39.469 565 302 14 44 588 262 806 8.51e-133 408
MS.gene067899.t1 MTR_5g080720 43.243 481 249 10 53 523 108 574 2.02e-132 399
MS.gene067899.t1 MTR_7g076150 43.601 461 232 8 139 588 1 444 5.80e-132 394
MS.gene067899.t1 MTR_5g083150 39.231 520 284 9 79 588 101 598 2.73e-130 395
MS.gene067899.t1 MTR_3g088790 40.571 525 282 11 79 588 233 742 7.73e-128 393
MS.gene067899.t1 MTR_1g051055 37.311 595 331 12 22 588 23 603 6.62e-126 384
MS.gene067899.t1 MTR_3g071530 40.000 520 276 11 79 588 153 646 8.87e-126 385
MS.gene067899.t1 MTR_1g022255 36.973 522 288 13 78 588 208 699 6.66e-107 337
MS.gene067899.t1 MTR_4g083030 32.914 556 315 12 59 585 75 601 2.88e-95 304
MS.gene067899.t1 MTR_3g074780 30.978 552 335 14 64 585 150 685 3.72e-93 301
MS.gene067899.t1 MTR_5g064080 31.365 542 320 13 73 585 149 667 1.13e-91 296
MS.gene067899.t1 MTR_1g022255 33.248 391 224 11 78 458 208 571 5.69e-60 210
MS.gene067899.t1 MTR_5g033760 45.455 66 36 0 531 596 41 106 2.19e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067899.t1 AT4G19120 73.782 595 145 3 7 596 10 598 0.0 947
MS.gene067899.t1 AT4G19120 73.782 595 145 3 7 596 10 598 0.0 947
MS.gene067899.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene067899.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene067899.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene067899.t1 AT1G31850 72.373 590 156 3 12 595 15 603 0.0 936
MS.gene067899.t1 AT4G19120 74.194 496 117 3 7 497 10 499 0.0 790
MS.gene067899.t1 AT4G00750 44.901 608 302 13 18 595 25 629 1.38e-180 525
MS.gene067899.t1 AT4G10440 46.429 532 276 6 70 594 93 622 9.81e-178 517
MS.gene067899.t1 AT2G45750 47.566 534 266 9 67 588 84 615 1.41e-175 512
MS.gene067899.t1 AT1G33170 46.679 527 271 7 70 588 110 634 6.72e-175 510
MS.gene067899.t1 AT4G18030 43.212 604 315 10 10 592 11 607 1.48e-171 501
MS.gene067899.t1 AT1G26850 44.853 544 286 8 61 595 77 615 2.51e-165 485
MS.gene067899.t1 AT1G26850 44.853 544 286 8 61 595 77 615 2.51e-165 485
MS.gene067899.t1 AT4G00740 44.023 527 267 8 73 588 84 593 2.09e-162 477
MS.gene067899.t1 AT4G00740 44.023 527 267 8 73 588 84 593 2.09e-162 477
MS.gene067899.t1 AT2G43200 45.800 500 264 7 70 565 91 587 5.42e-155 458
MS.gene067899.t1 AT2G43200 45.800 500 264 7 70 565 91 587 5.42e-155 458
MS.gene067899.t1 AT2G39750 43.831 543 280 11 56 587 163 691 3.35e-152 454
MS.gene067899.t1 AT1G77260 41.331 571 304 14 28 587 105 655 1.61e-146 438
MS.gene067899.t1 AT3G23300 39.138 603 330 8 16 591 12 604 1.07e-142 427
MS.gene067899.t1 AT3G23300 39.138 603 330 8 16 591 12 604 1.07e-142 427
MS.gene067899.t1 AT5G06050 40.741 540 291 11 61 587 140 663 1.54e-142 429
MS.gene067899.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene067899.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene067899.t1 AT1G04430 37.937 630 337 11 1 591 2 616 4.05e-142 426
MS.gene067899.t1 AT4G14360 41.341 537 291 7 69 591 75 601 2.11e-139 418
MS.gene067899.t1 AT4G14360 41.341 537 291 7 69 591 75 601 2.11e-139 418
MS.gene067899.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene067899.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene067899.t1 AT2G34300 42.468 551 279 15 56 588 227 757 3.37e-138 421
MS.gene067899.t1 AT2G40280 39.358 592 307 13 21 596 34 589 4.67e-138 414
MS.gene067899.t1 AT3G56080 43.636 495 253 13 79 564 114 591 1.88e-137 413
MS.gene067899.t1 AT1G29470 40.835 551 289 14 56 588 226 757 8.07e-136 414
MS.gene067899.t1 AT1G29470 40.835 551 289 14 56 588 226 757 8.07e-136 414
MS.gene067899.t1 AT5G64030 40.755 530 279 13 79 591 308 819 3.77e-132 407
MS.gene067899.t1 AT5G14430 39.000 600 324 11 17 588 19 604 1.59e-131 398
MS.gene067899.t1 AT5G14430 38.833 600 325 11 17 588 19 604 3.48e-130 395
MS.gene067899.t1 AT3G10200 36.198 605 345 12 6 588 3 588 1.62e-129 392
MS.gene067899.t1 AT3G51070 40.304 526 281 11 79 588 381 889 4.46e-128 398
MS.gene067899.t1 AT1G26850 43.765 425 227 6 61 478 77 496 2.79e-124 376
MS.gene067899.t1 AT3G56080 44.053 454 228 13 79 523 114 550 1.65e-123 375
MS.gene067899.t1 AT5G04060 36.227 599 337 13 17 588 17 597 6.48e-123 375
MS.gene067899.t1 AT3G10200 36.823 554 311 11 6 539 3 537 1.15e-117 362
MS.gene067899.t1 AT3G51070 42.105 456 234 10 79 518 381 822 5.69e-115 362
MS.gene067899.t1 AT1G19430 37.143 525 284 15 78 588 230 722 3.11e-108 341
MS.gene067899.t1 AT1G19430 37.143 525 284 15 78 588 230 722 3.11e-108 341
MS.gene067899.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene067899.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene067899.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene067899.t1 AT1G13860 32.246 552 308 14 70 587 81 600 1.64e-92 297
MS.gene067899.t1 AT1G78240 30.699 544 330 12 73 585 149 676 1.46e-89 291
MS.gene067899.t1 AT1G78240 30.699 544 330 12 73 585 149 676 1.46e-89 291
MS.gene067899.t1 AT2G03480 31.272 566 315 15 55 585 64 590 1.15e-85 278
MS.gene067899.t1 AT2G03480 31.150 565 328 15 55 585 64 601 1.28e-85 279
MS.gene067899.t1 AT1G13860 33.333 465 257 13 154 587 2 444 2.01e-79 258
MS.gene067899.t1 AT1G19430 34.937 395 215 13 78 459 230 595 5.15e-64 221
MS.gene067899.t1 AT2G03480 28.446 341 221 6 55 380 64 396 1.60e-42 159

Find 158 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCGTCCTGGAGGATTT+TGG 0.151747 8.2:+31964490 MS.gene067899:CDS
CTTATTCCATGGACAGAATT+TGG 0.162644 8.2:+31964308 MS.gene067899:CDS
ATAGTCCCATCTTTCATTTC+TGG 0.167085 8.2:-31963692 None:intergenic
TTCTATATGGGTGTAATCTT+TGG 0.198314 8.2:+31962797 MS.gene067899:CDS
CTGCTCTTTGGCCATCTAAT+TGG 0.211320 8.2:-31963405 None:intergenic
ATGTGGACTTTCTTTCTATA+TGG 0.220950 8.2:+31962784 MS.gene067899:CDS
CGGTAAATATGTTGATTTAA+TGG 0.261309 8.2:+31963658 MS.gene067899:CDS
CAATCAACATTGGTTGAAAA+AGG 0.293240 8.2:+31963583 MS.gene067899:CDS
CCCATCAAATTCCTTCGATA+TGG 0.309158 8.2:+31964268 MS.gene067899:CDS
ACTTATTGACGATGATTTCT+TGG 0.312151 8.2:-31962873 None:intergenic
CAAATTCTGTCCATGGAATA+AGG 0.319015 8.2:-31964307 None:intergenic
ATGGATATGAATACGGAATA+TGG 0.323237 8.2:+31964990 MS.gene067899:CDS
ACATATTTACCGACACCGTT+CGG 0.328019 8.2:-31963647 None:intergenic
TGTGGACTTTCTTTCTATAT+GGG 0.328602 8.2:+31962785 MS.gene067899:CDS
CAACATTCATCTCTGCTCTT+TGG 0.332417 8.2:-31963417 None:intergenic
TTATTGTAGCAGTCATTATC+AGG 0.334016 8.2:-31964675 None:intergenic
GGAGAGAAATTCCTCTTTCC+TGG 0.334164 8.2:+31963608 MS.gene067899:CDS
TAGCTGGGGTGGTGATTTGC+TGG 0.334950 8.2:+31964124 MS.gene067899:CDS
CGTGGTATCCCTGCAATTCT+TGG 0.339517 8.2:+31964218 MS.gene067899:CDS
CTGAGGCTGTCATCACATTT+AGG 0.345457 8.2:-31964720 None:intergenic
CATATTTACCGACACCGTTC+GGG 0.351894 8.2:-31963646 None:intergenic
AGCTCAGGTTCAATTTGCTT+TGG 0.352726 8.2:+31964193 MS.gene067899:CDS
CTTATTGACGATGATTTCTT+GGG 0.358122 8.2:-31962872 None:intergenic
ATTCGAACGGCCATTGATAC+TGG 0.358461 8.2:+31963713 MS.gene067899:CDS
TTACAGAGATGGAAGAAGTA+TGG 0.359526 8.2:+31963291 MS.gene067899:intron
TAGTCCCATCTTTCATTTCT+GGG 0.364638 8.2:-31963691 None:intergenic
TATGACCTCCTTCATCTTGC+AGG 0.378268 8.2:+31965262 MS.gene067899:CDS
AGCCACCAGGTCGTAAAATT+CGG 0.398973 8.2:-31965639 None:intergenic
GGAGTATACCATGCTGAATC+TGG 0.400239 8.2:-31964744 None:intergenic
TAAACCATTGAGATTCGTTC+TGG 0.401421 8.2:-31964858 None:intergenic
GATCCAAAGTTTAAGAAAAT+AGG 0.401896 8.2:+31964792 MS.gene067899:CDS
TGAAATGAAGTATGTGTTGC+TGG 0.403874 8.2:+31965609 MS.gene067899:CDS
GCAAGGACACCAAGAATTGC+AGG 0.407630 8.2:-31964227 None:intergenic
CCTTGATTGATGATCCTGTA+TGG 0.411232 8.2:+31965027 MS.gene067899:CDS
ATCTTATCAGTCCCAAGCTC+GGG 0.413990 8.2:-31964957 None:intergenic
TGATAGTCAATACTGCATTC+AGG 0.414829 8.2:-31962923 None:intergenic
TGATACAATTCTATCGTTCT+CGG 0.415528 8.2:-31962822 None:intergenic
GAAGTATGTGTTGCTGGAAA+TGG 0.420396 8.2:+31965615 MS.gene067899:CDS
AGGACAAAACAAGTGCGAAT+TGG 0.432966 8.2:-31964765 None:intergenic
ATCCTATCGGTTCATAATGT+TGG 0.442728 8.2:+31963314 MS.gene067899:CDS
ACGGCCATTGATACTGGTTG+TGG 0.443198 8.2:+31963719 MS.gene067899:CDS
GCTGGATCGTGGAATTCTTA+CGG 0.446421 8.2:+31964142 MS.gene067899:CDS
CTCCATGTCACCTCCTTGCT+CGG 0.448306 8.2:-31965749 None:intergenic
TCAGGAATGTTCCATTTGAC+TGG 0.450903 8.2:+31963543 MS.gene067899:intron
TGTTTGTTAATCCAGTCAAA+TGG 0.459476 8.2:-31963554 None:intergenic
TGTCCATGGAATAAGGCATC+TGG 0.467386 8.2:-31964300 None:intergenic
GGTACCAGCTTCCAATTAGA+TGG 0.467802 8.2:+31963394 MS.gene067899:CDS
ACCCTTGGCAATAGTTGTAA+TGG 0.468668 8.2:-31965698 None:intergenic
AAGAAGTTGATCCCCGAGCT+TGG 0.469511 8.2:+31964945 MS.gene067899:CDS
TTGGTGTCCTTGCTACTCAC+AGG 0.473805 8.2:+31964237 MS.gene067899:CDS
CTGATCCCAGAAATGAAAGA+TGG 0.476089 8.2:+31963686 MS.gene067899:CDS
GTTTATGATCGAGGCCTCAT+TGG 0.482541 8.2:+31965095 MS.gene067899:CDS
AAATGTGATGGATATGAATA+CGG 0.485516 8.2:+31964983 MS.gene067899:CDS
TCGAAGGAATTTGATGGGAA+TGG 0.487215 8.2:-31964263 None:intergenic
AGAAGTTGATCCCCGAGCTT+GGG 0.487598 8.2:+31964946 MS.gene067899:CDS
ATCCTCCAGGACGAAGAATC+CGG 0.487693 8.2:-31964485 None:intergenic
TGGTGTAACATGCAACCGCT+CGG 0.491978 8.2:-31964838 None:intergenic
CCCTGTGGCAATCATATTTG+TGG 0.495560 8.2:+31962757 MS.gene067899:CDS
GATCTGCGCTCGTAGTTGAT+TGG 0.499029 8.2:-31964528 None:intergenic
GAAGAATAATGAAGGAAAAG+TGG 0.501394 8.2:+31962715 MS.gene067899:CDS
GCCATATCGAAGGAATTTGA+TGG 0.506739 8.2:-31964269 None:intergenic
TGAGCTTGGTGGTTATCTCT+TGG 0.511910 8.2:-31964176 None:intergenic
AGCTGGTACCCATCCGGAGG+AGG 0.516830 8.2:-31963381 None:intergenic
TATCTTATCAGTCCCAAGCT+CGG 0.517834 8.2:-31964958 None:intergenic
GGTGTAACATGCAACCGCTC+GGG 0.518809 8.2:-31964837 None:intergenic
TCATTGGAACGTTCCACGAC+TGG 0.519029 8.2:+31965111 MS.gene067899:CDS
TGAAGAGTCCTGCAAGATGA+AGG 0.520104 8.2:-31965270 None:intergenic
TTGGCCATCTAATTGGAAGC+TGG 0.520718 8.2:-31963398 None:intergenic
ATTGGAAGCTGGTACCCATC+CGG 0.522262 8.2:-31963387 None:intergenic
CAAATTGAACCTGAGCTTGG+TGG 0.522756 8.2:-31964187 None:intergenic
TACACCAGAACGAATCTCAA+TGG 0.522832 8.2:+31964854 MS.gene067899:CDS
GCTCCAGATGCCTTATTCCA+TGG 0.526600 8.2:+31964297 MS.gene067899:CDS
TGCTTAGTTCCTCCTCCGGA+TGG 0.527581 8.2:+31963372 MS.gene067899:CDS
AAATTCCTCTTTCCTGGTGG+AGG 0.529642 8.2:+31963614 MS.gene067899:CDS
AGAATGCTTAGTTCCTCCTC+CGG 0.532704 8.2:+31963368 MS.gene067899:CDS
ACCACATTCATAACCCATAC+AGG 0.533600 8.2:-31965041 None:intergenic
AAGCAAATTGAACCTGAGCT+TGG 0.534577 8.2:-31964190 None:intergenic
AAGAGATAACCACCAAGCTC+AGG 0.536366 8.2:+31964178 MS.gene067899:CDS
GAAAAGTGGTAGACAAGAGT+AGG 0.538081 8.2:+31962729 MS.gene067899:CDS
TAGTACCTCCACCAGGAAAG+AGG 0.538108 8.2:-31963619 None:intergenic
CACTCCATGCACTGATCCAA+GGG 0.540931 8.2:+31962952 MS.gene067899:CDS
CACCCCACAACCAGTATCAA+TGG 0.542219 8.2:-31963723 None:intergenic
TGATCCCAGAAATGAAAGAT+GGG 0.543321 8.2:+31963687 MS.gene067899:CDS
GTAGTACCAAATTCTGTCCA+TGG 0.544289 8.2:-31964314 None:intergenic
CGGCCATTGATACTGGTTGT+GGG 0.544765 8.2:+31963720 MS.gene067899:CDS
GCAGAGATGAATGTTGGTAC+AGG 0.545315 8.2:+31963424 MS.gene067899:CDS
GTTCAATTTGCTTTGGAGCG+TGG 0.546321 8.2:+31964200 MS.gene067899:CDS
TGATAAGATAAGAAATGTGA+TGG 0.548109 8.2:+31964971 MS.gene067899:CDS
TATTTCATAATTACCAGTCG+TGG 0.552553 8.2:-31965124 None:intergenic
TGCCATTACAACTATTGCCA+AGG 0.553183 8.2:+31965696 MS.gene067899:CDS
ATCATATTTGTGGTGCTATG+TGG 0.553513 8.2:+31962767 MS.gene067899:CDS
CTGCGCTCGTAGTTGATTGG+TGG 0.554325 8.2:-31964525 None:intergenic
ACCACAAATATGATTGCCAC+AGG 0.556765 8.2:-31962758 None:intergenic
TTAATATTCTTGAAATACAC+CGG 0.559268 8.2:+31964466 MS.gene067899:CDS
ACACTCCATGCACTGATCCA+AGG 0.560389 8.2:+31962951 MS.gene067899:CDS
ATACACCGGATTCTTCGTCC+TGG 0.561320 8.2:+31964480 MS.gene067899:CDS
TACCGAGCAAGGAGGTGACA+TGG 0.562605 8.2:+31965747 MS.gene067899:CDS
TCAAACGTGACGGCAGTAAG+TGG 0.562817 8.2:+31964901 MS.gene067899:CDS
AACTACGAGCGCAGATCGCG+TGG 0.563131 8.2:+31964534 MS.gene067899:CDS
GTCATCACATTTAGGTGGAT+AGG 0.564284 8.2:-31964712 None:intergenic
AGGACGATGAAGAATAATGA+AGG 0.564405 8.2:+31962707 None:intergenic
ACTATGTTCCCGAACGGTGT+CGG 0.567956 8.2:+31963638 MS.gene067899:CDS
TCTCGAAAATTGGAGGGCAA+TGG 0.568556 8.2:-31963341 None:intergenic
ATCAGGAGACTTCTGCCATA+CGG 0.568603 8.2:-31964658 None:intergenic
CTCCCTGTGGTTTATGATCG+AGG 0.568992 8.2:+31965086 MS.gene067899:CDS
ACTGCATTCAGGAAAACTGA+TGG 0.569719 8.2:-31962912 None:intergenic
ACATTCCCATCGCATACCCT+TGG 0.569792 8.2:-31965713 None:intergenic
AGGCCTCGATCATAAACCAC+AGG 0.572832 8.2:-31965089 None:intergenic
TCTTCACTGCAGAAAGCCAC+AGG 0.573575 8.2:+31965287 MS.gene067899:CDS
CCATACAGGATCATCAATCA+AGG 0.575427 8.2:-31965027 None:intergenic
GACAAGACCCAAAATCCTCC+AGG 0.575555 8.2:-31964498 None:intergenic
GCTTAGTTCCTCCTCCGGAT+GGG 0.577164 8.2:+31963373 MS.gene067899:CDS
CAAAGAGCAGAGATGAATGT+TGG 0.578527 8.2:+31963418 MS.gene067899:CDS
ACGAGCGCAGATCGCGTGGA+TGG 0.579590 8.2:+31964538 MS.gene067899:CDS
TCTTAAACTTTGGATCCGGA+AGG 0.579696 8.2:-31964785 None:intergenic
CCATATCGAAGGAATTTGAT+GGG 0.583652 8.2:-31964268 None:intergenic
GGTGGTGATTTGCTGGATCG+TGG 0.586018 8.2:+31964131 MS.gene067899:CDS
CGAATCTCAATGGTTTATCG+TGG 0.588439 8.2:+31964864 MS.gene067899:CDS
CACCGGATTCTTCGTCCTGG+AGG 0.589366 8.2:+31964483 MS.gene067899:CDS
CTTGATTGATGATCCTGTAT+GGG 0.589605 8.2:+31965028 MS.gene067899:CDS
GTCTTGCATCAATTTCTAAG+TGG 0.591080 8.2:+31964814 MS.gene067899:CDS
TATTGCCAAGGGTATGCGAT+GGG 0.603217 8.2:+31965708 MS.gene067899:CDS
CGGATGAGAGCATAGCCACC+AGG 0.604150 8.2:-31965652 None:intergenic
GAGAAATTCCTCTTTCCTGG+TGG 0.605663 8.2:+31963611 MS.gene067899:CDS
AACAGAAGTCCAATCAACAT+TGG 0.605779 8.2:+31963573 MS.gene067899:CDS
ATTACCCTTGGATCAGTGCA+TGG 0.610417 8.2:-31962956 None:intergenic
TTATTGACGATGATTTCTTG+GGG 0.610889 8.2:-31962871 None:intergenic
CTATTGCCAAGGGTATGCGA+TGG 0.619397 8.2:+31965707 MS.gene067899:CDS
GAATCTCAATGGTTTATCGT+GGG 0.620901 8.2:+31964865 MS.gene067899:CDS
TATTGACGATGATTTCTTGG+GGG 0.626617 8.2:-31962870 None:intergenic
ATGGAAGCCTGTGAGTAGCA+AGG 0.627736 8.2:-31964244 None:intergenic
GGAAGAAGTATGGATCCTAT+CGG 0.630329 8.2:+31963301 MS.gene067899:CDS
TCCTGTATGGGTTATGAATG+TGG 0.630835 8.2:+31965040 MS.gene067899:CDS
TGCTGTGAATACACTCCCTG+TGG 0.631567 8.2:+31965073 MS.gene067899:CDS
AGGCTGTCATCACATTTAGG+TGG 0.634724 8.2:-31964717 None:intergenic
GAAAGATGGGACTATTCGAA+CGG 0.640093 8.2:+31963700 MS.gene067899:CDS
GCCATTACAACTATTGCCAA+GGG 0.640948 8.2:+31965697 MS.gene067899:CDS
GCCTCAGACCAGATTCAGCA+TGG 0.641516 8.2:+31964736 MS.gene067899:CDS
GGAGGTACTATGTTCCCGAA+CGG 0.648294 8.2:+31963632 MS.gene067899:CDS
TCTTATCAGTCCCAAGCTCG+GGG 0.654043 8.2:-31964956 None:intergenic
GATAGTACTTTCAAACGTGA+CGG 0.658518 8.2:+31964891 MS.gene067899:CDS
ACCATGCTGAATCTGGTCTG+AGG 0.664749 8.2:-31964737 None:intergenic
CAAGGACACCAAGAATTGCA+GGG 0.666290 8.2:-31964226 None:intergenic
GGCCATTGATACTGGTTGTG+GGG 0.668748 8.2:+31963721 MS.gene067899:CDS
CGTACAGAAGATACCGAGCA+AGG 0.670500 8.2:+31965736 MS.gene067899:CDS
TTCCAACATTATGAACCGAT+AGG 0.674489 8.2:-31963316 None:intergenic
CAATTTCTAAGTGGCCCGAG+CGG 0.675590 8.2:+31964823 MS.gene067899:CDS
GATATGAATACGGAATATGG+AGG 0.676561 8.2:+31964993 MS.gene067899:CDS
TGAAGGAGGTCATATGTCCG+AGG 0.677170 8.2:-31965253 None:intergenic
AGTCGTGGAACGTTCCAATG+AGG 0.677842 8.2:-31965109 None:intergenic
GGAAGCTGGTACCCATCCGG+AGG 0.678508 8.2:-31963384 None:intergenic
GGAGCAATGAGCCATATCGA+AGG 0.684039 8.2:-31964279 None:intergenic
AGAGTCCTGCAAGATGAAGG+AGG 0.687154 8.2:-31965267 None:intergenic
GGCCTCGATCATAAACCACA+GGG 0.703174 8.2:-31965088 None:intergenic
CAAGAGTAGGATCGTCCCTG+TGG 0.703595 8.2:+31962742 MS.gene067899:CDS
GGGAACATAGTACCTCCACC+AGG 0.712101 8.2:-31963626 None:intergenic
CCACAAATATGATTGCCACA+GGG 0.719216 8.2:-31962757 None:intergenic
ACAGAAGATACCGAGCAAGG+AGG 0.748039 8.2:+31965739 MS.gene067899:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTGTTATTAAAACAA+TGG + chr8.2:31965142-31965161 MS.gene067899:intron 10.0%
!!! ATTTGGTTTAAATATATTTT+TGG + chr8.2:31965353-31965372 MS.gene067899:intron 10.0%
!! TAAAGATTAAAATGATTACA+AGG + chr8.2:31965500-31965519 MS.gene067899:intron 15.0%
!! TATTTGTACACATTAATATT+TGG + chr8.2:31965336-31965355 MS.gene067899:intron 15.0%
!!! AAATTTGTTGATATTGAAAT+TGG + chr8.2:31965435-31965454 MS.gene067899:intron 15.0%
!!! ATTTTAATCTTTACTTTTAG+GGG - chr8.2:31965493-31965512 None:intergenic 15.0%
!!! CATTTTAATCTTTACTTTTA+GGG - chr8.2:31965494-31965513 None:intergenic 15.0%
!!! CTGATATTAATTGATTTTTT+AGG - chr8.2:31964028-31964047 None:intergenic 15.0%
!!! TCATTTTAATCTTTACTTTT+AGG - chr8.2:31965495-31965514 None:intergenic 15.0%
!! CCAAGATAATTTATAAAAAC+AGG + chr8.2:31963138-31963157 MS.gene067899:intron 20.0%
!! GTACAAATAACAAAATTAGT+TGG - chr8.2:31964006-31964025 None:intergenic 20.0%
!! TTAATATTCTTGAAATACAC+CGG + chr8.2:31964466-31964485 MS.gene067899:CDS 20.0%
!!! CCTGTTTTTATAAATTATCT+TGG - chr8.2:31963141-31963160 None:intergenic 20.0%
!!! GATTTGATTTTTGTTATGAT+AGG + chr8.2:31965209-31965228 MS.gene067899:intron 20.0%
!!! TATAGTTAATTTTACAGAGA+TGG + chr8.2:31963280-31963299 MS.gene067899:intron 20.0%
!!! TTTATCAGTGTTTTTTATCA+CGG + chr8.2:31963767-31963786 MS.gene067899:intron 20.0%
! ATAAGTTGTTTATCCAATCA+GGG + chr8.2:31963251-31963270 MS.gene067899:intron 25.0%
! CAGGAAACTTAAAATTATGT+CGG - chr8.2:31963487-31963506 None:intergenic 25.0%
! GATCCAAAGTTTAAGAAAAT+AGG + chr8.2:31964792-31964811 MS.gene067899:CDS 25.0%
! TAATATGCAAGAAAAAGCTA+TGG + chr8.2:31965779-31965798 MS.gene067899:CDS 25.0%
! TATAAGTTGTTTATCCAATC+AGG + chr8.2:31963250-31963269 MS.gene067899:intron 25.0%
! TGATAAGATAAGAAATGTGA+TGG + chr8.2:31964971-31964990 MS.gene067899:CDS 25.0%
!! AAAGAGAATTGTAATTTGCA+AGG - chr8.2:31963898-31963917 None:intergenic 25.0%
!! ACAAAACTCTTACTCATTTT+AGG + chr8.2:31964097-31964116 MS.gene067899:intron 25.0%
!! AGACCTATTTTCTTAAACTT+TGG - chr8.2:31964798-31964817 None:intergenic 25.0%
!! ATTTTCTTAAACTTTGGATC+CGG - chr8.2:31964792-31964811 None:intergenic 25.0%
!! CAATATCAACAAATTTTGCA+GGG - chr8.2:31965431-31965450 None:intergenic 25.0%
!! TAAAATGAGTAAGAGTTTTG+TGG - chr8.2:31964098-31964117 None:intergenic 25.0%
!! TCAATATCAACAAATTTTGC+AGG - chr8.2:31965432-31965451 None:intergenic 25.0%
!! TTGCTGTTATATCTAATTTC+AGG + chr8.2:31963525-31963544 MS.gene067899:intron 25.0%
!!! AAATGTGATGGATATGAATA+CGG + chr8.2:31964983-31965002 MS.gene067899:CDS 25.0%
!!! CGGTAAATATGTTGATTTAA+TGG + chr8.2:31963658-31963677 MS.gene067899:CDS 25.0%
AAATTATCTTGGACAGCTTA+TGG - chr8.2:31963130-31963149 None:intergenic 30.0%
AACACTGATAAAAACACAAG+AGG - chr8.2:31963759-31963778 None:intergenic 30.0%
ACTTATTGACGATGATTTCT+TGG - chr8.2:31962876-31962895 None:intergenic 30.0%
ATGGATATGAATACGGAATA+TGG + chr8.2:31964990-31965009 MS.gene067899:CDS 30.0%
ATGTGGACTTTCTTTCTATA+TGG + chr8.2:31962784-31962803 MS.gene067899:CDS 30.0%
CACTATACTAATATTACCCT+TGG - chr8.2:31962971-31962990 None:intergenic 30.0%
CATTAGAATAAGCTGTTTGT+TGG + chr8.2:31965313-31965332 MS.gene067899:intron 30.0%
CTGGTAAATGTTGCATAAAA+AGG - chr8.2:31964373-31964392 None:intergenic 30.0%
CTTATTGACGATGATTTCTT+GGG - chr8.2:31962875-31962894 None:intergenic 30.0%
GAAGAATAATGAAGGAAAAG+TGG + chr8.2:31962715-31962734 MS.gene067899:CDS 30.0%
GAGCTAGAATATGATTGTTA+AGG - chr8.2:31963921-31963940 None:intergenic 30.0%
GATACTTGTTTCATAGTTAC+TGG + chr8.2:31965540-31965559 MS.gene067899:intron 30.0%
TAACTACTATTCAACAACAC+TGG + chr8.2:31963854-31963873 MS.gene067899:intron 30.0%
TATTTCATAATTACCAGTCG+TGG - chr8.2:31965127-31965146 None:intergenic 30.0%
TGATACAATTCTATCGTTCT+CGG - chr8.2:31962825-31962844 None:intergenic 30.0%
TGTGGACTTTCTTTCTATAT+GGG + chr8.2:31962785-31962804 MS.gene067899:CDS 30.0%
TTAATGATCATGTGTCTGTT+TGG + chr8.2:31963033-31963052 MS.gene067899:intron 30.0%
TTATTGACGATGATTTCTTG+GGG - chr8.2:31962874-31962893 None:intergenic 30.0%
TTATTGTAGCAGTCATTATC+AGG - chr8.2:31964678-31964697 None:intergenic 30.0%
TTCATAGTTACTGGTATTTG+CGG + chr8.2:31965549-31965568 MS.gene067899:intron 30.0%
TTCTATATGGGTGTAATCTT+TGG + chr8.2:31962797-31962816 MS.gene067899:CDS 30.0%
TTCTGACAATGATTGTTACT+TGG + chr8.2:31965580-31965599 MS.gene067899:intron 30.0%
! AAAGTATTTGAGAGAGCTTA+TGG + chr8.2:31963062-31963081 MS.gene067899:intron 30.0%
! AATATTGCACCTTTTGCTTT+TGG + chr8.2:31965384-31965403 MS.gene067899:intron 30.0%
! CAATCAACATTGGTTGAAAA+AGG + chr8.2:31963583-31963602 MS.gene067899:CDS 30.0%
! CATTCTTTTCTCTCGAAAAT+TGG - chr8.2:31963354-31963373 None:intergenic 30.0%
! TGTTTGTTAATCCAGTCAAA+TGG - chr8.2:31963557-31963576 None:intergenic 30.0%
!! GTTGCGTTTTAATATGTCAT+GGG + chr8.2:31962990-31963009 MS.gene067899:intron 30.0%
!! TGTTGCGTTTTAATATGTCA+TGG + chr8.2:31962989-31963008 MS.gene067899:intron 30.0%
!!! AACAACACTGGTTTTTATGT+AGG + chr8.2:31963866-31963885 MS.gene067899:intron 30.0%
!!! ACAACACTGGTTTTTATGTA+GGG + chr8.2:31963867-31963886 MS.gene067899:intron 30.0%
!!! CTTTTTCAACCAATGTTGAT+TGG - chr8.2:31963585-31963604 None:intergenic 30.0%
AACAGAAGTCCAATCAACAT+TGG + chr8.2:31963573-31963592 MS.gene067899:CDS 35.0%
AACTATACTTAGACCCTGAT+TGG - chr8.2:31963267-31963286 None:intergenic 35.0%
AGATATAACAGCAACCATGA+CGG - chr8.2:31963519-31963538 None:intergenic 35.0%
ATAGTCCCATCTTTCATTTC+TGG - chr8.2:31963695-31963714 None:intergenic 35.0%
CAAATTCTGTCCATGGAATA+AGG - chr8.2:31964310-31964329 None:intergenic 35.0%
CCATATCGAAGGAATTTGAT+GGG - chr8.2:31964271-31964290 None:intergenic 35.0%
CTTATTCCATGGACAGAATT+TGG + chr8.2:31964308-31964327 MS.gene067899:CDS 35.0%
CTTGTAAAGTTCATGATCAC+TGG - chr8.2:31964392-31964411 None:intergenic 35.0%
GAATCTCAATGGTTTATCGT+GGG + chr8.2:31964865-31964884 MS.gene067899:CDS 35.0%
GATAGTACTTTCAAACGTGA+CGG + chr8.2:31964891-31964910 MS.gene067899:CDS 35.0%
GATATGAATACGGAATATGG+AGG + chr8.2:31964993-31965012 MS.gene067899:CDS 35.0%
GTCTTGCATCAATTTCTAAG+TGG + chr8.2:31964814-31964833 MS.gene067899:CDS 35.0%
TAAACCATTGAGATTCGTTC+TGG - chr8.2:31964861-31964880 None:intergenic 35.0%
TAGTAGTTATAGCGATGCTA+TGG - chr8.2:31963843-31963862 None:intergenic 35.0%
TAGTCCCATCTTTCATTTCT+GGG - chr8.2:31963694-31963713 None:intergenic 35.0%
TATTGACGATGATTTCTTGG+GGG - chr8.2:31962873-31962892 None:intergenic 35.0%
TCTCGCGATAGATAAAAGAA+TGG + chr8.2:31964061-31964080 MS.gene067899:intron 35.0%
TGAAATGAAGTATGTGTTGC+TGG + chr8.2:31965609-31965628 MS.gene067899:CDS 35.0%
TGATAGTCAATACTGCATTC+AGG - chr8.2:31962926-31962945 None:intergenic 35.0%
TGATCCCAGAAATGAAAGAT+GGG + chr8.2:31963687-31963706 MS.gene067899:CDS 35.0%
TTACAGAGATGGAAGAAGTA+TGG + chr8.2:31963291-31963310 MS.gene067899:intron 35.0%
TTCCAACATTATGAACCGAT+AGG - chr8.2:31963319-31963338 None:intergenic 35.0%
! AAGCATTTTCGACATATCCT+CGG + chr8.2:31965236-31965255 MS.gene067899:CDS 35.0%
! CAACATTGGTTGAAAAAGGA+AGG + chr8.2:31963587-31963606 MS.gene067899:CDS 35.0%
! CTTGATTGATGATCCTGTAT+GGG + chr8.2:31965028-31965047 MS.gene067899:CDS 35.0%
! CTTTTCTCTCGAAAATTGGA+GGG - chr8.2:31963350-31963369 None:intergenic 35.0%
! TCTTTTCTCTCGAAAATTGG+AGG - chr8.2:31963351-31963370 None:intergenic 35.0%
!! ATCATATTTGTGGTGCTATG+TGG + chr8.2:31962767-31962786 MS.gene067899:CDS 35.0%
!! ATCCTATCGGTTCATAATGT+TGG + chr8.2:31963314-31963333 MS.gene067899:CDS 35.0%
AAGCAAATTGAACCTGAGCT+TGG - chr8.2:31964193-31964212 None:intergenic 40.0%
ACATATTTACCGACACCGTT+CGG - chr8.2:31963650-31963669 None:intergenic 40.0%
ACCACAAATATGATTGCCAC+AGG - chr8.2:31962761-31962780 None:intergenic 40.0%
ACCACATTCATAACCCATAC+AGG - chr8.2:31965044-31965063 None:intergenic 40.0%
ACCCTTGGCAATAGTTGTAA+TGG - chr8.2:31965701-31965720 None:intergenic 40.0%
ACTGCATTCAGGAAAACTGA+TGG - chr8.2:31962915-31962934 None:intergenic 40.0%
AGGACAAAACAAGTGCGAAT+TGG - chr8.2:31964768-31964787 None:intergenic 40.0%
ATGATTTCTTGGGGGAATCA+AGG - chr8.2:31962865-31962884 None:intergenic 40.0%
CAAAGAGCAGAGATGAATGT+TGG + chr8.2:31963418-31963437 MS.gene067899:CDS 40.0%
CAACAAGGACCAAAAGCAAA+AGG - chr8.2:31965396-31965415 None:intergenic 40.0%
CAACATTCATCTCTGCTCTT+TGG - chr8.2:31963420-31963439 None:intergenic 40.0%
CCAAAAAGTAACTAGACTCG+CGG - chr8.2:31965675-31965694 None:intergenic 40.0%
CCACAAATATGATTGCCACA+GGG - chr8.2:31962760-31962779 None:intergenic 40.0%
CCATACAGGATCATCAATCA+AGG - chr8.2:31965030-31965049 None:intergenic 40.0%
CCCATCAAATTCCTTCGATA+TGG + chr8.2:31964268-31964287 MS.gene067899:CDS 40.0%
CGAATCTCAATGGTTTATCG+TGG + chr8.2:31964864-31964883 MS.gene067899:CDS 40.0%
CTGATCCCAGAAATGAAAGA+TGG + chr8.2:31963686-31963705 MS.gene067899:CDS 40.0%
GAAAAGTGGTAGACAAGAGT+AGG + chr8.2:31962729-31962748 MS.gene067899:CDS 40.0%
GAAAGATGGGACTATTCGAA+CGG + chr8.2:31963700-31963719 MS.gene067899:CDS 40.0%
GCCATATCGAAGGAATTTGA+TGG - chr8.2:31964272-31964291 None:intergenic 40.0%
GCCATTACAACTATTGCCAA+GGG + chr8.2:31965697-31965716 MS.gene067899:CDS 40.0%
GCTTATTCTAATGTCACCTG+TGG - chr8.2:31965306-31965325 None:intergenic 40.0%
GGGACTAATACAAGACAACA+AGG - chr8.2:31965411-31965430 None:intergenic 40.0%
GTAGTACCAAATTCTGTCCA+TGG - chr8.2:31964317-31964336 None:intergenic 40.0%
GTCATCACATTTAGGTGGAT+AGG - chr8.2:31964715-31964734 None:intergenic 40.0%
TACACCAGAACGAATCTCAA+TGG + chr8.2:31964854-31964873 MS.gene067899:CDS 40.0%
TATCTTATCAGTCCCAAGCT+CGG - chr8.2:31964961-31964980 None:intergenic 40.0%
TCAAGAATATTAACTCCGCC+TGG - chr8.2:31964459-31964478 None:intergenic 40.0%
TCAGGAATGTTCCATTTGAC+TGG + chr8.2:31963543-31963562 MS.gene067899:intron 40.0%
TCGAAGGAATTTGATGGGAA+TGG - chr8.2:31964266-31964285 None:intergenic 40.0%
TCTTAAACTTTGGATCCGGA+AGG - chr8.2:31964788-31964807 None:intergenic 40.0%
TGATTTCTTGGGGGAATCAA+GGG - chr8.2:31962864-31962883 None:intergenic 40.0%
TGCCATTACAACTATTGCCA+AGG + chr8.2:31965696-31965715 MS.gene067899:CDS 40.0%
! AGCTCAGGTTCAATTTGCTT+TGG + chr8.2:31964193-31964212 MS.gene067899:CDS 40.0%
! CCTTGATTGATGATCCTGTA+TGG + chr8.2:31965027-31965046 MS.gene067899:CDS 40.0%
! GAAGTATGTGTTGCTGGAAA+TGG + chr8.2:31965615-31965634 MS.gene067899:CDS 40.0%
! GGAAGAAGTATGGATCCTAT+CGG + chr8.2:31963301-31963320 MS.gene067899:CDS 40.0%
! TCACACTGCAATCTTTTACC+AGG + chr8.2:31964438-31964457 MS.gene067899:intron 40.0%
! TCCTGTATGGGTTATGAATG+TGG + chr8.2:31965040-31965059 MS.gene067899:CDS 40.0%
!!! ACTCATTTTAGGTTGCTAGC+TGG + chr8.2:31964108-31964127 MS.gene067899:intron 40.0%
!!! CTCATTTTAGGTTGCTAGCT+GGG + chr8.2:31964109-31964128 MS.gene067899:intron 40.0%
!!! GTGTTTTTTATCACGGAGCA+TGG + chr8.2:31963774-31963793 MS.gene067899:intron 40.0%
!!! TCATTTTAGGTTGCTAGCTG+GGG + chr8.2:31964110-31964129 MS.gene067899:intron 40.0%
AAAAAGGCGACATTGCCGTA+TGG + chr8.2:31964643-31964662 MS.gene067899:CDS 45.0%
AAATTCCTCTTTCCTGGTGG+AGG + chr8.2:31963614-31963633 MS.gene067899:CDS 45.0%
AAGAGATAACCACCAAGCTC+AGG + chr8.2:31964178-31964197 MS.gene067899:CDS 45.0%
AGAATGCTTAGTTCCTCCTC+CGG + chr8.2:31963368-31963387 MS.gene067899:CDS 45.0%
AGCCACCAGGTCGTAAAATT+CGG - chr8.2:31965642-31965661 None:intergenic 45.0%
AGGCTGTCATCACATTTAGG+TGG - chr8.2:31964720-31964739 None:intergenic 45.0%
ATCAGGAGACTTCTGCCATA+CGG - chr8.2:31964661-31964680 None:intergenic 45.0%
ATCTTATCAGTCCCAAGCTC+GGG - chr8.2:31964960-31964979 None:intergenic 45.0%
ATTACCCTTGGATCAGTGCA+TGG - chr8.2:31962959-31962978 None:intergenic 45.0%
ATTCGAACGGCCATTGATAC+TGG + chr8.2:31963713-31963732 MS.gene067899:CDS 45.0%
CAAATTGAACCTGAGCTTGG+TGG - chr8.2:31964190-31964209 None:intergenic 45.0%
CAAGGACACCAAGAATTGCA+GGG - chr8.2:31964229-31964248 None:intergenic 45.0%
CATATTTACCGACACCGTTC+GGG - chr8.2:31963649-31963668 None:intergenic 45.0%
CCCTGTGGCAATCATATTTG+TGG + chr8.2:31962757-31962776 MS.gene067899:CDS 45.0%
CTGAGGCTGTCATCACATTT+AGG - chr8.2:31964723-31964742 None:intergenic 45.0%
CTGCTCTTTGGCCATCTAAT+TGG - chr8.2:31963408-31963427 None:intergenic 45.0%
GAGAAATTCCTCTTTCCTGG+TGG + chr8.2:31963611-31963630 MS.gene067899:CDS 45.0%
GCAGAGATGAATGTTGGTAC+AGG + chr8.2:31963424-31963443 MS.gene067899:CDS 45.0%
GCTGGATCGTGGAATTCTTA+CGG + chr8.2:31964142-31964161 MS.gene067899:CDS 45.0%
GGAGAGAAATTCCTCTTTCC+TGG + chr8.2:31963608-31963627 MS.gene067899:CDS 45.0%
GGAGTATACCATGCTGAATC+TGG - chr8.2:31964747-31964766 None:intergenic 45.0%
GGTACCAGCTTCCAATTAGA+TGG + chr8.2:31963394-31963413 MS.gene067899:CDS 45.0%
GTTTATGATCGAGGCCTCAT+TGG + chr8.2:31965095-31965114 MS.gene067899:CDS 45.0%
TATCACGGAGCATGGAATAG+CGG + chr8.2:31963782-31963801 MS.gene067899:intron 45.0%
TATGACCTCCTTCATCTTGC+AGG + chr8.2:31965262-31965281 MS.gene067899:CDS 45.0%
TCTCGAAAATTGGAGGGCAA+TGG - chr8.2:31963344-31963363 None:intergenic 45.0%
TGAAGAGTCCTGCAAGATGA+AGG - chr8.2:31965273-31965292 None:intergenic 45.0%
TTGGCCATCTAATTGGAAGC+TGG - chr8.2:31963401-31963420 None:intergenic 45.0%
! ATGGACCGAATTTTACGACC+TGG + chr8.2:31965634-31965653 MS.gene067899:CDS 45.0%
! CACTGCAATCTTTTACCAGG+CGG + chr8.2:31964441-31964460 MS.gene067899:intron 45.0%
! GGAATATGGAGGTTTTGCTG+CGG + chr8.2:31965004-31965023 MS.gene067899:CDS 45.0%
! TATTGCCAAGGGTATGCGAT+GGG + chr8.2:31965708-31965727 MS.gene067899:CDS 45.0%
! TCTTCGTCCTGGAGGATTTT+GGG + chr8.2:31964491-31964510 MS.gene067899:CDS 45.0%
! TGAGCTTGGTGGTTATCTCT+TGG - chr8.2:31964179-31964198 None:intergenic 45.0%
! TGTCCATGGAATAAGGCATC+TGG - chr8.2:31964303-31964322 None:intergenic 45.0%
! TTCTTCGTCCTGGAGGATTT+TGG + chr8.2:31964490-31964509 MS.gene067899:CDS 45.0%
!! GTTCAATTTGCTTTGGAGCG+TGG + chr8.2:31964200-31964219 MS.gene067899:CDS 45.0%
!!! CCGCGAGTCTAGTTACTTTT+TGG + chr8.2:31965672-31965691 MS.gene067899:CDS 45.0%
!!! TCGCACTTGTTTTGTCCTTC+CGG + chr8.2:31964770-31964789 MS.gene067899:CDS 45.0%
!! TTTAAATTGTATAATAAAAA+AGG + chr8.2:31964627-31964646 MS.gene067899:CDS 5.0%
ACAGAAGATACCGAGCAAGG+AGG + chr8.2:31965739-31965758 MS.gene067899:CDS 50.0%
ACATTCCCATCGCATACCCT+TGG - chr8.2:31965716-31965735 None:intergenic 50.0%
ACCATGCTGAATCTGGTCTG+AGG - chr8.2:31964740-31964759 None:intergenic 50.0%
ACTATGTTCCCGAACGGTGT+CGG + chr8.2:31963638-31963657 MS.gene067899:CDS 50.0%
AGAGTCCTGCAAGATGAAGG+AGG - chr8.2:31965270-31965289 None:intergenic 50.0%
AGGCCTCGATCATAAACCAC+AGG - chr8.2:31965092-31965111 None:intergenic 50.0%
AGTCGTGGAACGTTCCAATG+AGG - chr8.2:31965112-31965131 None:intergenic 50.0%
ATACACCGGATTCTTCGTCC+TGG + chr8.2:31964480-31964499 MS.gene067899:CDS 50.0%
ATCCTCCAGGACGAAGAATC+CGG - chr8.2:31964488-31964507 None:intergenic 50.0%
ATGGAAGCCTGTGAGTAGCA+AGG - chr8.2:31964247-31964266 None:intergenic 50.0%
ATTGGAAGCTGGTACCCATC+CGG - chr8.2:31963390-31963409 None:intergenic 50.0%
CAATTTCTAAGTGGCCCGAG+CGG + chr8.2:31964823-31964842 MS.gene067899:CDS 50.0%
CACCCCACAACCAGTATCAA+TGG - chr8.2:31963726-31963745 None:intergenic 50.0%
CGTACAGAAGATACCGAGCA+AGG + chr8.2:31965736-31965755 MS.gene067899:CDS 50.0%
CGTGGTATCCCTGCAATTCT+TGG + chr8.2:31964218-31964237 MS.gene067899:CDS 50.0%
CTCCCTGTGGTTTATGATCG+AGG + chr8.2:31965086-31965105 MS.gene067899:CDS 50.0%
GACAAGACCCAAAATCCTCC+AGG - chr8.2:31964501-31964520 None:intergenic 50.0%
GATCTGCGCTCGTAGTTGAT+TGG - chr8.2:31964531-31964550 None:intergenic 50.0%
GCAAGGACACCAAGAATTGC+AGG - chr8.2:31964230-31964249 None:intergenic 50.0%
GGAGCAATGAGCCATATCGA+AGG - chr8.2:31964282-31964301 None:intergenic 50.0%
GGCCTCGATCATAAACCACA+GGG - chr8.2:31965091-31965110 None:intergenic 50.0%
TAGTACCTCCACCAGGAAAG+AGG - chr8.2:31963622-31963641 None:intergenic 50.0%
TCAAACGTGACGGCAGTAAG+TGG + chr8.2:31964901-31964920 MS.gene067899:CDS 50.0%
TCATTGGAACGTTCCACGAC+TGG + chr8.2:31965111-31965130 MS.gene067899:CDS 50.0%
TCCTGCAACTTACTCCGTCA+TGG + chr8.2:31963502-31963521 MS.gene067899:intron 50.0%
TCTTATCAGTCCCAAGCTCG+GGG - chr8.2:31964959-31964978 None:intergenic 50.0%
TCTTCACTGCAGAAAGCCAC+AGG + chr8.2:31965287-31965306 MS.gene067899:CDS 50.0%
TGAAGGAGGTCATATGTCCG+AGG - chr8.2:31965256-31965275 None:intergenic 50.0%
TGCTGTGAATACACTCCCTG+TGG + chr8.2:31965073-31965092 MS.gene067899:CDS 50.0%
TGGTGTAACATGCAACCGCT+CGG - chr8.2:31964841-31964860 None:intergenic 50.0%
! AAGAAGTTGATCCCCGAGCT+TGG + chr8.2:31964945-31964964 MS.gene067899:CDS 50.0%
! ACACTCCATGCACTGATCCA+AGG + chr8.2:31962951-31962970 MS.gene067899:CDS 50.0%
! ACCATGACGGAGTAAGTTGC+AGG - chr8.2:31963506-31963525 None:intergenic 50.0%
! ACGGCCATTGATACTGGTTG+TGG + chr8.2:31963719-31963738 MS.gene067899:CDS 50.0%
! AGAAGTTGATCCCCGAGCTT+GGG + chr8.2:31964946-31964965 MS.gene067899:CDS 50.0%
! CACTCCATGCACTGATCCAA+GGG + chr8.2:31962952-31962971 MS.gene067899:CDS 50.0%
! CGGCCATTGATACTGGTTGT+GGG + chr8.2:31963720-31963739 MS.gene067899:CDS 50.0%
! CTATTGCCAAGGGTATGCGA+TGG + chr8.2:31965707-31965726 MS.gene067899:CDS 50.0%
! GACCGAATTTTACGACCTGG+TGG + chr8.2:31965637-31965656 MS.gene067899:CDS 50.0%
! GCTCCAGATGCCTTATTCCA+TGG + chr8.2:31964297-31964316 MS.gene067899:CDS 50.0%
! GGCCATTGATACTGGTTGTG+GGG + chr8.2:31963721-31963740 MS.gene067899:CDS 50.0%
! TTGGTGTCCTTGCTACTCAC+AGG + chr8.2:31964237-31964256 MS.gene067899:CDS 50.0%
!! GGAGGATTTTGGGTCTTGTC+TGG + chr8.2:31964501-31964520 MS.gene067899:CDS 50.0%
!! GGAGGTACTATGTTCCCGAA+CGG + chr8.2:31963632-31963651 MS.gene067899:CDS 50.0%
!!! TTTTAGGTTGCTAGCTGGGG+TGG + chr8.2:31964113-31964132 MS.gene067899:intron 50.0%
CAAGAGTAGGATCGTCCCTG+TGG + chr8.2:31962742-31962761 MS.gene067899:CDS 55.0%
CTCCATGTCACCTCCTTGCT+CGG - chr8.2:31965752-31965771 None:intergenic 55.0%
CTGCGCTCGTAGTTGATTGG+TGG - chr8.2:31964528-31964547 None:intergenic 55.0%
GCCTCAGACCAGATTCAGCA+TGG + chr8.2:31964736-31964755 MS.gene067899:CDS 55.0%
GCTTAGTTCCTCCTCCGGAT+GGG + chr8.2:31963373-31963392 MS.gene067899:CDS 55.0%
GGGAACATAGTACCTCCACC+AGG - chr8.2:31963629-31963648 None:intergenic 55.0%
GGTGTAACATGCAACCGCTC+GGG - chr8.2:31964840-31964859 None:intergenic 55.0%
TACCGAGCAAGGAGGTGACA+TGG + chr8.2:31965747-31965766 MS.gene067899:CDS 55.0%
TGCTTAGTTCCTCCTCCGGA+TGG + chr8.2:31963372-31963391 MS.gene067899:CDS 55.0%
TGGGGGAATCAAGGGATCTC+TGG - chr8.2:31962856-31962875 None:intergenic 55.0%
! TAGCTGGGGTGGTGATTTGC+TGG + chr8.2:31964124-31964143 MS.gene067899:CDS 55.0%
!! GGTGGTGATTTGCTGGATCG+TGG + chr8.2:31964131-31964150 MS.gene067899:CDS 55.0%
AACTACGAGCGCAGATCGCG+TGG + chr8.2:31964534-31964553 MS.gene067899:CDS 60.0%
CACCGGATTCTTCGTCCTGG+AGG + chr8.2:31964483-31964502 MS.gene067899:CDS 60.0%
! CGGATGAGAGCATAGCCACC+AGG - chr8.2:31965655-31965674 None:intergenic 60.0%
ACGAGCGCAGATCGCGTGGA+TGG + chr8.2:31964538-31964557 MS.gene067899:CDS 65.0%
AGCTGGTACCCATCCGGAGG+AGG - chr8.2:31963384-31963403 None:intergenic 65.0%
GGAAGCTGGTACCCATCCGG+AGG - chr8.2:31963387-31963406 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 31962713 31965828 31962713 ID=MS.gene067899
chr8.2 mRNA 31962713 31965828 31962713 ID=MS.gene067899.t1;Parent=MS.gene067899
chr8.2 exon 31962713 31962973 31962713 ID=MS.gene067899.t1.exon1;Parent=MS.gene067899.t1
chr8.2 CDS 31962713 31962973 31962713 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31963297 31963445 31963297 ID=MS.gene067899.t1.exon2;Parent=MS.gene067899.t1
chr8.2 CDS 31963297 31963445 31963297 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31963547 31963742 31963547 ID=MS.gene067899.t1.exon3;Parent=MS.gene067899.t1
chr8.2 CDS 31963547 31963742 31963547 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31964119 31964329 31964119 ID=MS.gene067899.t1.exon4;Parent=MS.gene067899.t1
chr8.2 CDS 31964119 31964329 31964119 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31964460 31965132 31964460 ID=MS.gene067899.t1.exon5;Parent=MS.gene067899.t1
chr8.2 CDS 31964460 31965132 31964460 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31965231 31965308 31965231 ID=MS.gene067899.t1.exon6;Parent=MS.gene067899.t1
chr8.2 CDS 31965231 31965308 31965231 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
chr8.2 exon 31965606 31965828 31965606 ID=MS.gene067899.t1.exon7;Parent=MS.gene067899.t1
chr8.2 CDS 31965606 31965828 31965606 ID=cds.MS.gene067899.t1;Parent=MS.gene067899.t1
Gene Sequence

>MS.gene067899

ATGAAGAATAATGAAGGAAAAGTGGTAGACAAGAGTAGGATCGTCCCTGTGGCAATCATATTTGTGGTGCTATGTGGACTTTCTTTCTATATGGGTGTAATCTTTGGTTCCGAGAACGATAGAATTGTATCAATTATCAGCCAGAGATCCCTTGATTCCCCCAAGAAATCATCGTCAATAAGTTCTCTGCAAATTAAATCCATCAGTTTTCCTGAATGCAGTATTGACTATCAAGATTACACTCCATGCACTGATCCAAGGGTAATATTAGTATAGTGTTGCGTTTTAATATGTCATGGGATTTGTAGAAGAAAGTTTTCTTAATGATCATGTGTCTGTTTGGATCGTAAAAGTATTTGAGAGAGCTTATGGAAACAACTTATGATATGTCTATAAGTTGTTTTCAGTTTATTTCCATAAGCTGTCCAAGATAATTTATAAAAACAGGCTATATCTTGTATAAAAACAATTTGTCTTTATTTTATCTTTTATTATAGATATAGCTTATTCACAATCATTTACAATAAGCTCTTATGCTATAAGTTGTTTATCCAATCAGGGTCTAAGTATAGTTAATTTTACAGAGATGGAAGAAGTATGGATCCTATCGGTTCATAATGTTGGAACGCCATTGCCCTCCAATTTTCGAGAGAAAAGAATGCTTAGTTCCTCCTCCGGATGGGTACCAGCTTCCAATTAGATGGCCAAAGAGCAGAGATGAATGTTGGTACAGGTAATTATATAAGTCTGATGACGAATGATAATATAAGACCGACATAATTTTAAGTTTCCTGCAACTTACTCCGTCATGGTTGCTGTTATATCTAATTTCAGGAATGTTCCATTTGACTGGATTAACAAACAGAAGTCCAATCAACATTGGTTGAAAAAGGAAGGAGAGAAATTCCTCTTTCCTGGTGGAGGTACTATGTTCCCGAACGGTGTCGGTAAATATGTTGATTTAATGGAAGATCTGATCCCAGAAATGAAAGATGGGACTATTCGAACGGCCATTGATACTGGTTGTGGGGTGAGTCTTGCTTCCTCTTGTGTTTTTATCAGTGTTTTTTATCACGGAGCATGGAATAGCGGTATGCTCAAATTCTGCTACACTACAGTCTACAGCGCCATAGCATCGCTATAACTACTATTCAACAACACTGGTTTTTATGTAGGGAATAGCCTTGCAAATTACAATTCTCTTTCCTTAACAATCATATTCTAGCTCAATTATATGTGCTTCTTCTTGCAGTTGTGCTTTTCTAACTACATTGTAAATATATTTTAAAACCAACTAATTTTGTTATTTGTACCTAAAAAATCAATTAATATCAGTTGCAATTTTCAATCTCGCGATAGATAAAAGAATGGCATCAATTAATCCACAAAACTCTTACTCATTTTAGGTTGCTAGCTGGGGTGGTGATTTGCTGGATCGTGGAATTCTTACGGTTTCTCTTGCACCAAGAGATAACCACCAAGCTCAGGTTCAATTTGCTTTGGAGCGTGGTATCCCTGCAATTCTTGGTGTCCTTGCTACTCACAGGCTTCCATTCCCATCAAATTCCTTCGATATGGCTCATTGCTCCAGATGCCTTATTCCATGGACAGAATTTGGTACTACTATGAATTTTGCTCTTAATCAAACTGAATTAATCCTTTTTATGCAACATTTACCAGTGATCATGAACTTTACAAGTCTTGTAGCTTCTTAAACTTTAAAATTCACACTGCAATCTTTTACCAGGCGGAGTTAATATTCTTGAAATACACCGGATTCTTCGTCCTGGAGGATTTTGGGTCTTGTCTGGTCCACCAATCAACTACGAGCGCAGATCGCGTGGATGGAACACAACTATCGAAGAGCAAAGATCAACTTACGAGAAATTGCAGAACTTACTGACTTCATTGTGCTTTAAATTGTATAATAAAAAAGGCGACATTGCCGTATGGCAGAAGTCTCCTGATAATGACTGCTACAATAAGCTGACTAGAGATACCTATCCACCTAAATGTGATGACAGCCTCAGACCAGATTCAGCATGGTATACTCCAATTCGCACTTGTTTTGTCCTTCCGGATCCAAAGTTTAAGAAAATAGGTCTTGCATCAATTTCTAAGTGGCCCGAGCGGTTGCATGTTACACCAGAACGAATCTCAATGGTTTATCGTGGGAGTGATAGTACTTTCAAACGTGACGGCAGTAAGTGGAAGAAGCATGTCGCACACTACAAGAAGTTGATCCCCGAGCTTGGGACTGATAAGATAAGAAATGTGATGGATATGAATACGGAATATGGAGGTTTTGCTGCGGCCTTGATTGATGATCCTGTATGGGTTATGAATGTGGTCTCATCTTATGCTGTGAATACACTCCCTGTGGTTTATGATCGAGGCCTCATTGGAACGTTCCACGACTGGTAATTATGAAATATTGTTATTAAAACAATGGTTTTATTTCTGTATATAATTTTATCTTATAAATAATCTACTGTTGATTTGATTTTTGTTATGATAGGTGTGAAGCATTTTCGACATATCCTCGGACATATGACCTCCTTCATCTTGCAGGACTCTTCACTGCAGAAAGCCACAGGTGACATTAGAATAAGCTGTTTGTTGGTATTTGTACACATTAATATTTGGTTTAAATATATTTTTGGTCTTTGCAAATATTGCACCTTTTGCTTTTGGTCCTTGTTGTCTTGTATTAGTCCCTGCAAAATTTGTTGATATTGAAATTGGTGTTTGTAATATGTAAAATATTTGATTTTAGTCCCCTAAAAGTAAAGATTAAAATGATTACAAGGCTAATTTGATAAGTGATGATACTTGTTTCATAGTTACTGGTATTTGCGGCTGTTTCATTCTGACAATGATTGTTACTTGGCAGATGTGAAATGAAGTATGTGTTGCTGGAAATGGACCGAATTTTACGACCTGGTGGCTATGCTCTCATCCGCGAGTCTAGTTACTTTTTGGATGCCATTACAACTATTGCCAAGGGTATGCGATGGGAATGTCGTACAGAAGATACCGAGCAAGGAGGTGACATGGAGAAGATACTAATATGCAAGAAAAAGCTATGGTATTCATCAAATAAAGATTCAAGATGA
Protein sequence

>MS.gene067899.t1

MKNNEGKVVDKSRIVPVAIIFVVLCGLSFYMGVIFGSENDRIVSIISQRSLDSPKKSSSISSLQIKSISFPECSIDYQDYTPCTDPRRWKKYGSYRFIMLERHCPPIFERKECLVPPPDGYQLPIRWPKSRDECWYRNVPFDWINKQKSNQHWLKKEGEKFLFPGGGTMFPNGVGKYVDLMEDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHQAQVQFALERGIPAILGVLATHRLPFPSNSFDMAHCSRCLIPWTEFGGVNILEIHRILRPGGFWVLSGPPINYERRSRGWNTTIEEQRSTYEKLQNLLTSLCFKLYNKKGDIAVWQKSPDNDCYNKLTRDTYPPKCDDSLRPDSAWYTPIRTCFVLPDPKFKKIGLASISKWPERLHVTPERISMVYRGSDSTFKRDGSKWKKHVAHYKKLIPELGTDKIRNVMDMNTEYGGFAAALIDDPVWVMNVVSSYAVNTLPVVYDRGLIGTFHDWCEAFSTYPRTYDLLHLAGLFTAESHRCEMKYVLLEMDRILRPGGYALIRESSYFLDAITTIAKGMRWECRTEDTEQGGDMEKILICKKKLWYSSNKDSR