AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056880.t1 MTR_1g059890 96.786 280 9 0 1 280 1 280 0.0 549
MS.gene056880.t1 MTR_7g104725 43.226 310 121 13 3 280 1 287 3.52e-49 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056880.t1 AT2G36220 36.177 293 136 11 1 274 1 261 2.47e-30 115
MS.gene056880.t1 AT3G52710 35.976 328 107 18 1 269 1 284 1.10e-29 114

Find 78 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGCGGAGTTAGGGTTTA+TGG 0.148476 1.1:-45658044 None:intergenic
TTGGTTCGCAAGCGACTTTC+CGG 0.232258 1.1:-45657901 None:intergenic
CACAGTGAACCGGCCGGTTT+GGG 0.244338 1.1:+45658592 MS.gene056880:CDS
TGAGAGTTTGAAGCGGAGTT+AGG 0.333425 1.1:-45658052 None:intergenic
TCACAGTGAACCGGCCGGTT+TGG 0.337046 1.1:+45658591 MS.gene056880:CDS
AACGTAGCTTTCTGTGATTT+CGG 0.349811 1.1:-45658127 None:intergenic
GGGGCGGCGGTCCGAGTCAT+GGG 0.355455 1.1:+45658636 MS.gene056880:CDS
ACTCACCACCGGAATCATTC+TGG 0.363382 1.1:+45657939 MS.gene056880:CDS
GGAAACAACCTCGTGTTTCC+CGG 0.372036 1.1:-45658187 None:intergenic
ACCGCCGCCCCGACGCAAAC+CGG 0.376641 1.1:-45658624 None:intergenic
CGGGGCGGCGGTCCGAGTCA+TGG 0.386264 1.1:+45658635 MS.gene056880:CDS
CGCCGGATAAGGCGGAAACT+AGG 0.395537 1.1:-45657868 None:intergenic
ACTCAAAGAAAGAAGAAAAC+TGG 0.400989 1.1:+45658406 MS.gene056880:CDS
TCGCCGAAGGTTTCTTGTAT+AGG 0.403972 1.1:+45658265 MS.gene056880:CDS
TCGATGAGCCGGTTTGCGTC+GGG 0.405928 1.1:+45658616 MS.gene056880:CDS
TCCACCGGAAGATTCGATTT+CGG 0.409789 1.1:-45658484 None:intergenic
ACCCTAGTTTCCGCCTTATC+CGG 0.413514 1.1:+45657866 MS.gene056880:CDS
GTCGGCGGCAACGCCGGATA+AGG 0.415027 1.1:-45657879 None:intergenic
CCGATCGACGCACTTCGTTT+CGG 0.421050 1.1:-45658208 None:intergenic
TTCGATGAGCCGGTTTGCGT+CGG 0.422675 1.1:+45658615 MS.gene056880:CDS
CCGGTTTGGGTTCGATGAGC+CGG 0.433092 1.1:+45658605 MS.gene056880:CDS
TTAGAGAGCCAGAATGATTC+CGG 0.442874 1.1:-45657947 None:intergenic
CAAGAAACCTTCGGCGAAGA+AGG 0.455115 1.1:-45658259 None:intergenic
GCCGGTTTGCGTCGGGGCGG+CGG 0.460288 1.1:+45658623 MS.gene056880:CDS
CCGGCTCATCGAACCCAAAC+CGG 0.463824 1.1:-45658605 None:intergenic
TATCCGGCGTTGCCGCCGAC+CGG 0.472326 1.1:+45657882 MS.gene056880:CDS
GCCGGATAAGGCGGAAACTA+GGG 0.475520 1.1:-45657867 None:intergenic
GAGAGTTTGAAGCGGAGTTA+GGG 0.479960 1.1:-45658051 None:intergenic
GGCAGCGGAGACGCCGGTAG+CGG 0.484213 1.1:-45658353 None:intergenic
TTTCCGGTCGGCGGCAACGC+CGG 0.485958 1.1:-45657885 None:intergenic
TGTTCCGAAATCGAATCTTC+CGG 0.502511 1.1:+45658480 MS.gene056880:CDS
TCGATTTCTTCCACCGCTAC+CGG 0.504664 1.1:+45658340 MS.gene056880:CDS
GCTACCGGCGTCTCCGCTGC+CGG 0.507701 1.1:+45658355 MS.gene056880:CDS
CTGTCTTACAACGCCGGCAG+CGG 0.516489 1.1:-45658368 None:intergenic
TTCGTTGAAGAATCTTCCAC+CGG 0.530549 1.1:-45658499 None:intergenic
GAGTCTTCCGCCTCATCAGT+TGG 0.531012 1.1:-45657920 None:intergenic
TGATTCGTTTCTTTCGTAGA+CGG 0.541509 1.1:-45658002 None:intergenic
GAGTAGGACGGTTGAAGAGA+CGG 0.543360 1.1:+45658567 MS.gene056880:CDS
TCAAAAGCTACGACAATCAT+CGG 0.554027 1.1:+45658100 MS.gene056880:CDS
TTCACCTACTCCCCATGACT+CGG 0.557110 1.1:-45658647 None:intergenic
AGCGGAGACGCCGGTAGCGG+TGG 0.558353 1.1:-45658350 None:intergenic
CGTCGGAATCATCACAAGCC+GGG 0.559044 1.1:+45658169 MS.gene056880:CDS
CTACCTATACAAGAAACCTT+CGG 0.563783 1.1:-45658268 None:intergenic
AACGCCGGCAGCGGAGACGC+CGG 0.571035 1.1:-45658359 None:intergenic
AGAGACGGTTCACAGTGAAC+CGG 0.571066 1.1:+45658582 MS.gene056880:CDS
TTCGCAAGCGACTTTCCGGT+CGG 0.571303 1.1:-45657897 None:intergenic
CGGTCCGAGTCATGGGGAGT+AGG 0.577370 1.1:+45658643 MS.gene056880:CDS
CCGTCGGAATCATCACAAGC+CGG 0.577564 1.1:+45658168 MS.gene056880:CDS
ACGAAGAAGAAGAGAAACAA+TGG 0.589286 1.1:+45658517 MS.gene056880:CDS
GGCGGCAACGCCGGATAAGG+CGG 0.589432 1.1:-45657876 None:intergenic
CTCATCGAACCCAAACCGGC+CGG 0.591441 1.1:-45658601 None:intergenic
ACGACGCTGAATACGACTGG+TGG 0.592186 1.1:+45657969 MS.gene056880:CDS
CCGAAACGAAGTGCGTCGAT+CGG 0.602094 1.1:+45658208 MS.gene056880:CDS
CTAACGACGCTGAATACGAC+TGG 0.603633 1.1:+45657966 MS.gene056880:CDS
GCAAGCGACTTTCCGGTCGG+CGG 0.604992 1.1:-45657894 None:intergenic
GGGCGGCGGTCCGAGTCATG+GGG 0.607070 1.1:+45658637 MS.gene056880:CDS
CGATGAGCCGGTTTGCGTCG+GGG 0.607545 1.1:+45658617 MS.gene056880:CDS
AAACGAAGTGCGTCGATCGG+AGG 0.610287 1.1:+45658211 MS.gene056880:CDS
CGACGCTGAATACGACTGGT+GGG 0.619893 1.1:+45657970 MS.gene056880:CDS
GCTACGTTAGCAGAAGCTAG+AGG 0.623845 1.1:+45658142 MS.gene056880:CDS
CGCTTGCGAACCAACTGATG+AGG 0.624098 1.1:+45657910 MS.gene056880:CDS
CCGGCTTGTGATGATTCCGA+CGG 0.625963 1.1:-45658168 None:intergenic
GGGAAGGAAGTACGGTGAGT+AGG 0.628019 1.1:+45658551 MS.gene056880:CDS
TTGCGAACCAACTGATGAGG+CGG 0.641142 1.1:+45657913 MS.gene056880:CDS
AGATTTCTGTCTTACAACGC+CGG 0.641306 1.1:-45658374 None:intergenic
GAATCGTTGAGAGTTTGAAG+CGG 0.651586 1.1:-45658059 None:intergenic
ACGGTTCACAGTGAACCGGC+CGG 0.657233 1.1:+45658586 MS.gene056880:CDS
AGGAAGTACGGTGAGTAGGA+CGG 0.657574 1.1:+45658555 MS.gene056880:CDS
CGAAACGTGGACATAATCGA+AGG 0.662504 1.1:+45658299 MS.gene056880:CDS
CAGAAGCTAGAGGCTGCCGT+CGG 0.668462 1.1:+45658152 MS.gene056880:CDS
TCCGAAATCGAATCTTCCGG+TGG 0.683823 1.1:+45658483 MS.gene056880:CDS
TGAGCCGGTTTGCGTCGGGG+CGG 0.691949 1.1:+45658620 MS.gene056880:CDS
AGGGTTTCGAAATCTAGTTG+TGG 0.692327 1.1:-45657848 None:intergenic
TGAGGCGGAAGACTCACCAC+CGG 0.696003 1.1:+45657928 MS.gene056880:CDS
ATCACAAGCCGGGAAACACG+AGG 0.698693 1.1:+45658179 MS.gene056880:CDS
GAGAGCCAGAATGATTCCGG+TGG 0.708692 1.1:-45657944 None:intergenic
GCAGCCACCGAATTCACACG+TGG 0.735786 1.1:-45658678 None:intergenic
GGTAGAGTGAGATCGAAACG+TGG 0.747418 1.1:+45658286 MS.gene056880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATTTCTTCAAATTCT+TGG - chr1.1:45658086-45658105 None:intergenic 20.0%
ACTCAAAGAAAGAAGAAAAC+TGG + chr1.1:45658406-45658425 MS.gene056880:CDS 30.0%
AAACAATGGAAGAAAAACGC+GGG + chr1.1:45658531-45658550 MS.gene056880:CDS 35.0%
AACGTAGCTTTCTGTGATTT+CGG - chr1.1:45658130-45658149 None:intergenic 35.0%
ACGAAGAAGAAGAGAAACAA+TGG + chr1.1:45658517-45658536 MS.gene056880:CDS 35.0%
CTACCTATACAAGAAACCTT+CGG - chr1.1:45658271-45658290 None:intergenic 35.0%
GAAACAATGGAAGAAAAACG+CGG + chr1.1:45658530-45658549 MS.gene056880:CDS 35.0%
TCAAAAGCTACGACAATCAT+CGG + chr1.1:45658100-45658119 MS.gene056880:CDS 35.0%
TGATTCGTTTCTTTCGTAGA+CGG - chr1.1:45658005-45658024 None:intergenic 35.0%
AGATTTCTGTCTTACAACGC+CGG - chr1.1:45658377-45658396 None:intergenic 40.0%
AGGGTTTCGAAATCTAGTTG+TGG - chr1.1:45657851-45657870 None:intergenic 40.0%
GAATCGTTGAGAGTTTGAAG+CGG - chr1.1:45658062-45658081 None:intergenic 40.0%
TGTTCCGAAATCGAATCTTC+CGG + chr1.1:45658480-45658499 MS.gene056880:CDS 40.0%
TTAGAGAGCCAGAATGATTC+CGG - chr1.1:45657950-45657969 None:intergenic 40.0%
TTCGTTGAAGAATCTTCCAC+CGG - chr1.1:45658502-45658521 None:intergenic 40.0%
AAAAACGCGGGAAGGAAGTA+CGG + chr1.1:45658543-45658562 MS.gene056880:CDS 45.0%
AATGGAAGAAAAACGCGGGA+AGG + chr1.1:45658535-45658554 MS.gene056880:CDS 45.0%
CGAAACGTGGACATAATCGA+AGG + chr1.1:45658299-45658318 MS.gene056880:CDS 45.0%
TCCACCGGAAGATTCGATTT+CGG - chr1.1:45658487-45658506 None:intergenic 45.0%
TCGCCGAAGGTTTCTTGTAT+AGG + chr1.1:45658265-45658284 MS.gene056880:CDS 45.0%
TGAAGCGGAGTTAGGGTTTA+TGG - chr1.1:45658047-45658066 None:intergenic 45.0%
! GAGAGTTTGAAGCGGAGTTA+GGG - chr1.1:45658054-45658073 None:intergenic 45.0%
! TCGGAACAGTTTTCTCTCTC+CGG - chr1.1:45658468-45658487 None:intergenic 45.0%
! TGAGAGTTTGAAGCGGAGTT+AGG - chr1.1:45658055-45658074 None:intergenic 45.0%
! TTTTGAACCTTCTTCGCCGA+AGG + chr1.1:45658252-45658271 MS.gene056880:CDS 45.0%
!! AAAGCGTTTGCGCGATTTTC+AGG + chr1.1:45658437-45658456 MS.gene056880:CDS 45.0%
ACCCTAGTTTCCGCCTTATC+CGG + chr1.1:45657866-45657885 MS.gene056880:CDS 50.0%
ACTCACCACCGGAATCATTC+TGG + chr1.1:45657939-45657958 MS.gene056880:CDS 50.0%
AGAGACGGTTCACAGTGAAC+CGG + chr1.1:45658582-45658601 MS.gene056880:CDS 50.0%
CAAGAAACCTTCGGCGAAGA+AGG - chr1.1:45658262-45658281 None:intergenic 50.0%
CTAACGACGCTGAATACGAC+TGG + chr1.1:45657966-45657985 MS.gene056880:CDS 50.0%
GAGTAGGACGGTTGAAGAGA+CGG + chr1.1:45658567-45658586 MS.gene056880:CDS 50.0%
GCTACGTTAGCAGAAGCTAG+AGG + chr1.1:45658142-45658161 MS.gene056880:CDS 50.0%
GGAAACAACCTCGTGTTTCC+CGG - chr1.1:45658190-45658209 None:intergenic 50.0%
GGTAGAGTGAGATCGAAACG+TGG + chr1.1:45658286-45658305 MS.gene056880:CDS 50.0%
TCCGAAATCGAATCTTCCGG+TGG + chr1.1:45658483-45658502 MS.gene056880:CDS 50.0%
TCGATTTCTTCCACCGCTAC+CGG + chr1.1:45658340-45658359 MS.gene056880:CDS 50.0%
TTCACCTACTCCCCATGACT+CGG - chr1.1:45658650-45658669 None:intergenic 50.0%
TTGCGAACCAACTGATGAGG+CGG + chr1.1:45657913-45657932 MS.gene056880:CDS 50.0%
! AGGAAGTACGGTGAGTAGGA+CGG + chr1.1:45658555-45658574 MS.gene056880:CDS 50.0%
! TTGGTTCGCAAGCGACTTTC+CGG - chr1.1:45657904-45657923 None:intergenic 50.0%
ACGACGCTGAATACGACTGG+TGG + chr1.1:45657969-45657988 MS.gene056880:CDS 55.0%
ATCACAAGCCGGGAAACACG+AGG + chr1.1:45658179-45658198 MS.gene056880:CDS 55.0%
CCGATCGACGCACTTCGTTT+CGG - chr1.1:45658211-45658230 None:intergenic 55.0%
CCGTCGGAATCATCACAAGC+CGG + chr1.1:45658168-45658187 MS.gene056880:CDS 55.0%
CGACGCTGAATACGACTGGT+GGG + chr1.1:45657970-45657989 MS.gene056880:CDS 55.0%
CGCTTGCGAACCAACTGATG+AGG + chr1.1:45657910-45657929 MS.gene056880:CDS 55.0%
CGTCGGAATCATCACAAGCC+GGG + chr1.1:45658169-45658188 MS.gene056880:CDS 55.0%
GAGAGCCAGAATGATTCCGG+TGG - chr1.1:45657947-45657966 None:intergenic 55.0%
GAGTCTTCCGCCTCATCAGT+TGG - chr1.1:45657923-45657942 None:intergenic 55.0%
GCCGGATAAGGCGGAAACTA+GGG - chr1.1:45657870-45657889 None:intergenic 55.0%
TTCGCAAGCGACTTTCCGGT+CGG - chr1.1:45657900-45657919 None:intergenic 55.0%
! AAACGAAGTGCGTCGATCGG+AGG + chr1.1:45658211-45658230 MS.gene056880:CDS 55.0%
! CCGAAACGAAGTGCGTCGAT+CGG + chr1.1:45658208-45658227 MS.gene056880:CDS 55.0%
! CCGGCTTGTGATGATTCCGA+CGG - chr1.1:45658171-45658190 None:intergenic 55.0%
! GGGAAGGAAGTACGGTGAGT+AGG + chr1.1:45658551-45658570 MS.gene056880:CDS 55.0%
!! GCGCGATTTTCAGGAGTCAC+CGG + chr1.1:45658446-45658465 MS.gene056880:CDS 55.0%
!! TTCGATGAGCCGGTTTGCGT+CGG + chr1.1:45658615-45658634 MS.gene056880:CDS 55.0%
ACGGTTCACAGTGAACCGGC+CGG + chr1.1:45658586-45658605 MS.gene056880:CDS 60.0%
CAGAAGCTAGAGGCTGCCGT+CGG + chr1.1:45658152-45658171 MS.gene056880:CDS 60.0%
CCGGCTCATCGAACCCAAAC+CGG - chr1.1:45658608-45658627 None:intergenic 60.0%
CGCCGGATAAGGCGGAAACT+AGG - chr1.1:45657871-45657890 None:intergenic 60.0%
CTCATCGAACCCAAACCGGC+CGG - chr1.1:45658604-45658623 None:intergenic 60.0%
CTGTCTTACAACGCCGGCAG+CGG - chr1.1:45658371-45658390 None:intergenic 60.0%
TCACAGTGAACCGGCCGGTT+TGG + chr1.1:45658591-45658610 MS.gene056880:CDS 60.0%
TGAGGCGGAAGACTCACCAC+CGG + chr1.1:45657928-45657947 MS.gene056880:CDS 60.0%
! CACAGTGAACCGGCCGGTTT+GGG + chr1.1:45658592-45658611 MS.gene056880:CDS 60.0%
!! CCGGTTTGGGTTCGATGAGC+CGG + chr1.1:45658605-45658624 MS.gene056880:CDS 60.0%
!! TCGATGAGCCGGTTTGCGTC+GGG + chr1.1:45658616-45658635 MS.gene056880:CDS 60.0%
CGGTCCGAGTCATGGGGAGT+AGG + chr1.1:45658643-45658662 MS.gene056880:CDS 65.0%
GCAAGCGACTTTCCGGTCGG+CGG - chr1.1:45657897-45657916 None:intergenic 65.0%
!! CGATGAGCCGGTTTGCGTCG+GGG + chr1.1:45658617-45658636 MS.gene056880:CDS 65.0%
GGCGGCAACGCCGGATAAGG+CGG - chr1.1:45657879-45657898 None:intergenic 70.0%
GTCGGCGGCAACGCCGGATA+AGG - chr1.1:45657882-45657901 None:intergenic 70.0%
TATCCGGCGTTGCCGCCGAC+CGG + chr1.1:45657882-45657901 MS.gene056880:CDS 70.0%
TTTCCGGTCGGCGGCAACGC+CGG - chr1.1:45657888-45657907 None:intergenic 70.0%
!! TGAGCCGGTTTGCGTCGGGG+CGG + chr1.1:45658620-45658639 MS.gene056880:CDS 70.0%
AACGCCGGCAGCGGAGACGC+CGG - chr1.1:45658362-45658381 None:intergenic 75.0%
ACCGCCGCCCCGACGCAAAC+CGG - chr1.1:45658627-45658646 None:intergenic 75.0%
GCTACCGGCGTCTCCGCTGC+CGG + chr1.1:45658355-45658374 MS.gene056880:CDS 75.0%
GGCAGCGGAGACGCCGGTAG+CGG - chr1.1:45658356-45658375 None:intergenic 75.0%
GGGCGGCGGTCCGAGTCATG+GGG + chr1.1:45658637-45658656 MS.gene056880:CDS 75.0%
GGGGCGGCGGTCCGAGTCAT+GGG + chr1.1:45658636-45658655 MS.gene056880:CDS 75.0%
! AGCGGAGACGCCGGTAGCGG+TGG - chr1.1:45658353-45658372 None:intergenic 75.0%
! CGGGGCGGCGGTCCGAGTCA+TGG + chr1.1:45658635-45658654 MS.gene056880:CDS 80.0%
!!! GCCGGTTTGCGTCGGGGCGG+CGG + chr1.1:45658623-45658642 MS.gene056880:CDS 80.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 45657845 45658687 45657845 ID=MS.gene056880
chr1.1 mRNA 45657845 45658687 45657845 ID=MS.gene056880.t1;Parent=MS.gene056880
chr1.1 exon 45657845 45658687 45657845 ID=MS.gene056880.t1.exon1;Parent=MS.gene056880.t1
chr1.1 CDS 45657845 45658687 45657845 ID=cds.MS.gene056880.t1;Parent=MS.gene056880.t1
Gene Sequence

>MS.gene056880

ATGCCACAACTAGATTTCGAAACCCTAGTTTCCGCCTTATCCGGCGTTGCCGCCGACCGGAAAGTCGCTTGCGAACCAACTGATGAGGCGGAAGACTCACCACCGGAATCATTCTGGCTCTCTAACGACGCTGAATACGACTGGTGGGACAGAAACGCCGTCTACGAAAGAAACGAATCAACAAAAGCAAGCTCAATTTCCATAAACCCTAACTCCGCTTCAAACTCTCAACGATTCTCCAAGAATTTGAAGAAATCAAAAGCTACGACAATCATCGGTTTACCGAAATCACAGAAAGCTACGTTAGCAGAAGCTAGAGGCTGCCGTCGGAATCATCACAAGCCGGGAAACACGAGGTTGTTTCCGAAACGAAGTGCGTCGATCGGAGGAAAATCTGATTCTACTGTTTTTGAACCTTCTTCGCCGAAGGTTTCTTGTATAGGTAGAGTGAGATCGAAACGTGGACATAATCGAAGGTTGAGAACTCGTCAGAGATCGATTTCTTCCACCGCTACCGGCGTCTCCGCTGCCGGCGTTGTAAGACAGAAATCTTCACGTAGTACTCAAAGAAAGAAGAAAACTGGTTTTATCGAAAGCGTTTGCGCGATTTTCAGGAGTCACCGGAGAGAGAAAACTGTTCCGAAATCGAATCTTCCGGTGGAAGATTCTTCAACGAAGAAGAAGAGAAACAATGGAAGAAAAACGCGGGAAGGAAGTACGGTGAGTAGGACGGTTGAAGAGACGGTTCACAGTGAACCGGCCGGTTTGGGTTCGATGAGCCGGTTTGCGTCGGGGCGGCGGTCCGAGTCATGGGGAGTAGGTGAAATTGAAAACCACGTGTGA

Protein sequence

>MS.gene056880.t1

MPQLDFETLVSALSGVAADRKVACEPTDEAEDSPPESFWLSNDAEYDWWDRNAVYERNESTKASSISINPNSASNSQRFSKNLKKSKATTIIGLPKSQKATLAEARGCRRNHHKPGNTRLFPKRSASIGGKSDSTVFEPSSPKVSCIGRVRSKRGHNRRLRTRQRSISSTATGVSAAGVVRQKSSRSTQRKKKTGFIESVCAIFRSHRREKTVPKSNLPVEDSSTKKKRNNGRKTREGSTVSRTVEETVHSEPAGLGSMSRFASGRRSESWGVGEIENHV