Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46106.t1 | AFK47532.1 | 96.5 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 1.20E-38 | 168.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46106.t1 | I3T4U0 | 96.5 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 8.5e-39 | 168.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene46106 | -0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene049611 | MS.gene46106 | 0.8122 | 4.78E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene46106 | -0.826057 | 3.34E-54 | -1.69E-46 |
| MS.gene050380 | MS.gene46106 | 0.858134 | 1.01E-62 | -1.69E-46 |
| MS.gene050381 | MS.gene46106 | 0.860233 | 2.38E-63 | -1.69E-46 |
| MS.gene050868 | MS.gene46106 | -0.826892 | 2.11E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene46106 | -0.8266 | 2.48E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene46106 | 0.823504 | 1.34E-53 | -1.69E-46 |
| MS.gene051268 | MS.gene46106 | -0.805147 | 1.54E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene46106 | 0.811318 | 7.43E-51 | -1.69E-46 |
| MS.gene051366 | MS.gene46106 | 0.806927 | 6.49E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene46106 | 0.814944 | 1.19E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene46106 | 0.846902 | 1.62E-59 | -1.69E-46 |
| MS.gene05234 | MS.gene46106 | 0.813649 | 2.30E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene46106 | -0.821616 | 3.67E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene46106 | 0.861904 | 7.39E-64 | -1.69E-46 |
| MS.gene053561 | MS.gene46106 | 0.828728 | 7.65E-55 | -1.69E-46 |
| MS.gene054003 | MS.gene46106 | -0.806197 | 9.25E-50 | -1.69E-46 |
| MS.gene054707 | MS.gene46106 | 0.806328 | 8.68E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene46106 | 0.840295 | 9.48E-58 | -1.69E-46 |
| MS.gene055124 | MS.gene46106 | 0.828901 | 6.95E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene46106 | 0.823294 | 1.50E-53 | -1.69E-46 |
| MS.gene055202 | MS.gene46106 | 0.923396 | 2.81E-89 | -1.69E-46 |
| MS.gene056298 | MS.gene46106 | 0.806524 | 7.89E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene46106 | 0.831269 | 1.84E-55 | -1.69E-46 |
| MS.gene056311 | MS.gene46106 | 0.842128 | 3.12E-58 | -1.69E-46 |
| MS.gene056313 | MS.gene46106 | 0.844768 | 6.16E-59 | -1.69E-46 |
| MS.gene056504 | MS.gene46106 | -0.80735 | 5.28E-50 | -1.69E-46 |
| MS.gene056880 | MS.gene46106 | 0.814019 | 1.91E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene46106 | 0.858317 | 8.95E-63 | -1.69E-46 |
| MS.gene057173 | MS.gene46106 | 0.819772 | 9.74E-53 | -1.69E-46 |
| MS.gene057473 | MS.gene46106 | 0.813667 | 2.28E-51 | -1.69E-46 |
| MS.gene058041 | MS.gene46106 | -0.825013 | 5.91E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene46106 | 0.814511 | 1.48E-51 | -1.69E-46 |
| MS.gene058108 | MS.gene46106 | 0.808452 | 3.08E-50 | -1.69E-46 |
| MS.gene058109 | MS.gene46106 | 0.812091 | 5.05E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene46106 | -0.814027 | 1.90E-51 | -1.69E-46 |
| MS.gene058170 | MS.gene46106 | 0.808778 | 2.62E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene46106 | 0.878737 | 2.20E-69 | -1.69E-46 |
| MS.gene058173 | MS.gene46106 | 0.870824 | 1.09E-66 | -1.69E-46 |
| MS.gene058174 | MS.gene46106 | 0.807495 | 4.92E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene46106 | 0.878323 | 3.08E-69 | -1.69E-46 |
| MS.gene05837 | MS.gene46106 | -0.80642 | 8.30E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene46106 | 0.829243 | 5.74E-55 | -1.69E-46 |
| MS.gene058494 | MS.gene46106 | 0.820422 | 6.92E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene46106 | 0.804526 | 2.07E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene46106 | 0.812785 | 3.56E-51 | -1.69E-46 |
| MS.gene05884 | MS.gene46106 | 0.801585 | 8.40E-49 | -1.69E-46 |
| MS.gene059037 | MS.gene46106 | 0.809079 | 2.26E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene46106 | 0.800178 | 1.63E-48 | -1.69E-46 |
| MS.gene059045 | MS.gene46106 | 0.810118 | 1.35E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene46106 | 0.815418 | 9.34E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene46106 | -0.800178 | 1.63E-48 | -1.69E-46 |
| MS.gene060187 | MS.gene46106 | 0.838527 | 2.73E-57 | -1.69E-46 |
| MS.gene060595 | MS.gene46106 | 0.871082 | 8.96E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46106.t1 | MTR_7g063490 | 96.512 | 86 | 3 | 0 | 1 | 86 | 1 | 86 | 7.44e-58 | 172 |
| MS.gene46106.t1 | MTR_4g021330 | 55.056 | 89 | 36 | 3 | 1 | 85 | 1 | 89 | 1.33e-22 | 83.6 |
| MS.gene46106.t1 | MTR_2g104750 | 50.000 | 86 | 38 | 4 | 1 | 81 | 1 | 86 | 4.29e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGTTTCTTCTTCTACTTC+AGG | 0.270205 | 7.2:+41290326 | MS.gene46106:CDS |
| AGACTTCCTACTGGTGATTC+CGG | 0.399839 | 7.2:+41290269 | MS.gene46106:CDS |
| GCACAGATCATTGTTTCATC+TGG | 0.416986 | 7.2:+41290350 | MS.gene46106:CDS |
| ATTATTGTCTTGTAAACTAA+AGG | 0.417225 | 7.2:-41290176 | None:intergenic |
| GGTGGTAAAGAAGGTGCTAT+AGG | 0.442411 | 7.2:+41290209 | MS.gene46106:CDS |
| CAATACAAAGGTGGTAAAGA+AGG | 0.510069 | 7.2:+41290200 | MS.gene46106:CDS |
| AACATGGTTGGAAGATGAAG+TGG | 0.530962 | 7.2:-41290309 | None:intergenic |
| TTCCTACTGGTGATTCCGGT+CGG | 0.535055 | 7.2:+41290273 | MS.gene46106:CDS |
| AAGAAGGTGCTATAGGATCA+TGG | 0.541472 | 7.2:+41290216 | MS.gene46106:CDS |
| AGTCTCATGTATGAATATGA+AGG | 0.564487 | 7.2:-41290251 | None:intergenic |
| GGTTTGCATTTGAAACCGAC+CGG | 0.571028 | 7.2:-41290288 | None:intergenic |
| AGTAGAAGAAGAAACATGGT+TGG | 0.588464 | 7.2:-41290321 | None:intergenic |
| TCATACATGAGACTTCCTAC+TGG | 0.612878 | 7.2:+41290260 | MS.gene46106:CDS |
| CTGAAGTAGAAGAAGAAACA+TGG | 0.621219 | 7.2:-41290325 | None:intergenic |
| TCTCACACATCGTCGAATCG+CGG | 0.623740 | 7.2:+41290394 | MS.gene46106:CDS |
| CGATGTGTGAGACAATTATG+TGG | 0.634825 | 7.2:-41290383 | None:intergenic |
| ACAATAATGCAATACAAAGG+TGG | 0.639646 | 7.2:+41290191 | MS.gene46106:CDS |
| AAGACAATAATGCAATACAA+AGG | 0.668198 | 7.2:+41290188 | MS.gene46106:CDS |
| AACCGACCGGAATCACCAGT+AGG | 0.722417 | 7.2:-41290275 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTGTCTTGTAAACTAA+AGG | - | chr7.2:41290179-41290198 | None:intergenic | 20.0% |
| ! | AAGACAATAATGCAATACAA+AGG | + | chr7.2:41290188-41290207 | MS.gene46106:CDS | 25.0% |
| ACAATAATGCAATACAAAGG+TGG | + | chr7.2:41290191-41290210 | MS.gene46106:CDS | 30.0% | |
| AGTCTCATGTATGAATATGA+AGG | - | chr7.2:41290254-41290273 | None:intergenic | 30.0% | |
| AGTAGAAGAAGAAACATGGT+TGG | - | chr7.2:41290324-41290343 | None:intergenic | 35.0% | |
| CAATACAAAGGTGGTAAAGA+AGG | + | chr7.2:41290200-41290219 | MS.gene46106:CDS | 35.0% | |
| CATGTTTCTTCTTCTACTTC+AGG | + | chr7.2:41290326-41290345 | MS.gene46106:CDS | 35.0% | |
| CTGAAGTAGAAGAAGAAACA+TGG | - | chr7.2:41290328-41290347 | None:intergenic | 35.0% | |
| AACATGGTTGGAAGATGAAG+TGG | - | chr7.2:41290312-41290331 | None:intergenic | 40.0% | |
| CGATGTGTGAGACAATTATG+TGG | - | chr7.2:41290386-41290405 | None:intergenic | 40.0% | |
| GCACAGATCATTGTTTCATC+TGG | + | chr7.2:41290350-41290369 | MS.gene46106:CDS | 40.0% | |
| TCATACATGAGACTTCCTAC+TGG | + | chr7.2:41290260-41290279 | MS.gene46106:CDS | 40.0% | |
| !! | AAGAAGGTGCTATAGGATCA+TGG | + | chr7.2:41290216-41290235 | MS.gene46106:CDS | 40.0% |
| AGACTTCCTACTGGTGATTC+CGG | + | chr7.2:41290269-41290288 | MS.gene46106:CDS | 45.0% | |
| ! | GGTTTGCATTTGAAACCGAC+CGG | - | chr7.2:41290291-41290310 | None:intergenic | 45.0% |
| !! | GGTGGTAAAGAAGGTGCTAT+AGG | + | chr7.2:41290209-41290228 | MS.gene46106:CDS | 45.0% |
| TCTCACACATCGTCGAATCG+CGG | + | chr7.2:41290394-41290413 | MS.gene46106:CDS | 50.0% | |
| TTCCTACTGGTGATTCCGGT+CGG | + | chr7.2:41290273-41290292 | MS.gene46106:CDS | 50.0% | |
| AACCGACCGGAATCACCAGT+AGG | - | chr7.2:41290278-41290297 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 41290161 | 41290421 | 41290161 | ID=MS.gene46106 |
| chr7.2 | mRNA | 41290161 | 41290421 | 41290161 | ID=MS.gene46106.t1;Parent=MS.gene46106 |
| chr7.2 | exon | 41290161 | 41290421 | 41290161 | ID=MS.gene46106.t1.exon1;Parent=MS.gene46106.t1 |
| chr7.2 | CDS | 41290161 | 41290421 | 41290161 | ID=cds.MS.gene46106.t1;Parent=MS.gene46106.t1 |
| Gene Sequence |
| Protein sequence |
