Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89803.t1 | AFK47532.1 | 93 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 7.70E-38 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89803.t1 | I3T4U0 | 93.0 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 5.5e-38 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene89803 | -0.80212 | 6.52E-49 | -1.69E-46 |
| MS.gene049232 | MS.gene89803 | -0.811298 | 7.51E-51 | -1.69E-46 |
| MS.gene049281 | MS.gene89803 | 0.817269 | 3.60E-52 | -1.69E-46 |
| MS.gene049483 | MS.gene89803 | 0.812298 | 4.55E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene89803 | 0.829672 | 4.51E-55 | -1.69E-46 |
| MS.gene049634 | MS.gene89803 | 0.810783 | 9.71E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene89803 | -0.802902 | 4.50E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene89803 | -0.805139 | 1.54E-49 | -1.69E-46 |
| MS.gene050216 | MS.gene89803 | -0.836458 | 9.25E-57 | -1.69E-46 |
| MS.gene050380 | MS.gene89803 | 0.841181 | 5.55E-58 | -1.69E-46 |
| MS.gene050381 | MS.gene89803 | 0.862689 | 4.24E-64 | -1.69E-46 |
| MS.gene050496 | MS.gene89803 | -0.80939 | 1.94E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene89803 | -0.813135 | 2.98E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene89803 | -0.809029 | 2.32E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene89803 | -0.839713 | 1.34E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene89803 | -0.840897 | 6.59E-58 | -1.69E-46 |
| MS.gene050883 | MS.gene89803 | 0.810326 | 1.22E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene89803 | 0.806786 | 6.95E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene89803 | 0.813796 | 2.13E-51 | -1.69E-46 |
| MS.gene051127 | MS.gene89803 | 0.837479 | 5.07E-57 | -1.69E-46 |
| MS.gene051138 | MS.gene89803 | 0.830279 | 3.21E-55 | -1.69E-46 |
| MS.gene051268 | MS.gene89803 | -0.806282 | 8.87E-50 | -1.69E-46 |
| MS.gene051364 | MS.gene89803 | 0.850431 | 1.71E-60 | -1.69E-46 |
| MS.gene051366 | MS.gene89803 | 0.847055 | 1.47E-59 | -1.69E-46 |
| MS.gene051367 | MS.gene89803 | 0.826259 | 2.99E-54 | -1.69E-46 |
| MS.gene05141 | MS.gene89803 | 0.814253 | 1.69E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene89803 | 0.844064 | 9.52E-59 | -1.69E-46 |
| MS.gene052211 | MS.gene89803 | 0.812996 | 3.20E-51 | -1.69E-46 |
| MS.gene05234 | MS.gene89803 | 0.816081 | 6.64E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene89803 | -0.816589 | 5.12E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene89803 | 0.868534 | 6.08E-66 | -1.69E-46 |
| MS.gene053561 | MS.gene89803 | 0.844675 | 6.53E-59 | -1.69E-46 |
| MS.gene053562 | MS.gene89803 | 0.801878 | 7.31E-49 | -1.69E-46 |
| MS.gene054003 | MS.gene89803 | -0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene054054 | MS.gene89803 | 0.806234 | 9.08E-50 | -1.69E-46 |
| MS.gene054616 | MS.gene89803 | 0.816582 | 5.13E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene89803 | 0.822321 | 2.52E-53 | -1.69E-46 |
| MS.gene054707 | MS.gene89803 | 0.813415 | 2.59E-51 | -1.69E-46 |
| MS.gene054708 | MS.gene89803 | 0.843759 | 1.15E-58 | -1.69E-46 |
| MS.gene055124 | MS.gene89803 | 0.856686 | 2.72E-62 | -1.69E-46 |
| MS.gene055139 | MS.gene89803 | 0.82614 | 3.19E-54 | -1.69E-46 |
| MS.gene055154 | MS.gene89803 | 0.867281 | 1.54E-65 | -1.69E-46 |
| MS.gene055202 | MS.gene89803 | 0.952227 | 3.81E-110 | -1.69E-46 |
| MS.gene055502 | MS.gene89803 | -0.800797 | 1.22E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene89803 | -0.819608 | 1.06E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene89803 | -0.82101 | 5.07E-53 | -1.69E-46 |
| MS.gene056310 | MS.gene89803 | 0.83335 | 5.61E-56 | -1.69E-46 |
| MS.gene056311 | MS.gene89803 | 0.819587 | 1.07E-52 | -1.69E-46 |
| MS.gene056313 | MS.gene89803 | 0.833704 | 4.58E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene89803 | -0.833323 | 5.70E-56 | -1.69E-46 |
| MS.gene056880 | MS.gene89803 | 0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene89803 | 0.833455 | 5.28E-56 | -1.69E-46 |
| MS.gene057473 | MS.gene89803 | 0.846201 | 2.52E-59 | -1.69E-46 |
| MS.gene058068 | MS.gene89803 | 0.819921 | 9.01E-53 | -1.69E-46 |
| MS.gene058093 | MS.gene89803 | 0.809294 | 2.03E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene89803 | 0.829278 | 5.63E-55 | -1.69E-46 |
| MS.gene058108 | MS.gene89803 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene058109 | MS.gene89803 | 0.825492 | 4.55E-54 | -1.69E-46 |
| MS.gene058157 | MS.gene89803 | -0.829682 | 4.49E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene89803 | 0.870402 | 1.50E-66 | -1.69E-46 |
| MS.gene058173 | MS.gene89803 | 0.853654 | 2.07E-61 | -1.69E-46 |
| MS.gene058175 | MS.gene89803 | 0.866269 | 3.22E-65 | -1.69E-46 |
| MS.gene058268 | MS.gene89803 | 0.836466 | 9.20E-57 | -1.69E-46 |
| MS.gene058269 | MS.gene89803 | 0.84324 | 1.58E-58 | -1.69E-46 |
| MS.gene05837 | MS.gene89803 | -0.825352 | 4.91E-54 | -1.69E-46 |
| MS.gene058493 | MS.gene89803 | 0.821245 | 4.47E-53 | -1.69E-46 |
| MS.gene058494 | MS.gene89803 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene89803 | -0.808016 | 3.81E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene89803 | 0.8008 | 1.22E-48 | -1.69E-46 |
| MS.gene058978 | MS.gene89803 | 0.801007 | 1.10E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene89803 | -0.818128 | 2.30E-52 | -1.69E-46 |
| MS.gene059037 | MS.gene89803 | 0.824532 | 7.67E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene89803 | 0.86059 | 1.86E-63 | -1.69E-46 |
| MS.gene059865 | MS.gene89803 | -0.832135 | 1.12E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene89803 | -0.802997 | 4.30E-49 | -1.69E-46 |
| MS.gene059916 | MS.gene89803 | 0.824263 | 8.87E-54 | -1.69E-46 |
| MS.gene060187 | MS.gene89803 | 0.804004 | 2.66E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene89803 | -0.818313 | 2.09E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene89803 | 0.824229 | 9.04E-54 | -1.69E-46 |
| MS.gene060595 | MS.gene89803 | 0.86357 | 2.27E-64 | -1.69E-46 |
| MS.gene060762 | MS.gene89803 | -0.804593 | 2.01E-49 | -1.69E-46 |
| MS.gene060778 | MS.gene89803 | -0.82235 | 2.48E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene89803 | -0.813265 | 2.79E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89803.t1 | MTR_7g063490 | 93.023 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 1.05e-46 | 144 |
| MS.gene89803.t1 | MTR_4g021330 | 55.056 | 89 | 36 | 3 | 1 | 85 | 1 | 89 | 1.31e-23 | 85.9 |
| MS.gene89803.t1 | MTR_2g104750 | 50.000 | 86 | 38 | 4 | 1 | 81 | 1 | 86 | 3.85e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACTTCCAACCGGTGATTC+CGG | 0.332515 | 7.3:-41740298 | MS.gene89803:CDS |
| AATGGAGGGTCTTATACCTT+TGG | 0.361131 | 7.3:-41740407 | MS.gene89803:CDS |
| GCACAAATCATTGTTTCATC+TGG | 0.387174 | 7.3:-41740217 | MS.gene89803:CDS |
| GGTGGTAAAGAAGGTGCTAT+AGG | 0.442411 | 7.3:-41740358 | MS.gene89803:CDS |
| AAGAAGGTGCTATAGGATCA+TGG | 0.480205 | 7.3:-41740351 | MS.gene89803:CDS |
| TTGAAACCGACCGGAATCAC+CGG | 0.491223 | 7.3:+41740288 | None:intergenic |
| CAATACAAAGGTGGTAAAGA+AGG | 0.512508 | 7.3:-41740367 | MS.gene89803:CDS |
| AACATGGTTGGAAGATGAAG+TGG | 0.530962 | 7.3:+41740258 | None:intergenic |
| ATTATTGTCTTGTAAACCAA+AGG | 0.536810 | 7.3:+41740391 | None:intergenic |
| AGAAGGTGCTATAGGATCAT+GGG | 0.557929 | 7.3:-41740350 | MS.gene89803:CDS |
| GGTTTGCATTTGAAACCGAC+CGG | 0.571028 | 7.3:+41740279 | None:intergenic |
| TCATACATGAGACTTCCAAC+CGG | 0.604318 | 7.3:-41740307 | MS.gene89803:CDS |
| TTCCAACCGGTGATTCCGGT+CGG | 0.604554 | 7.3:-41740294 | MS.gene89803:CDS |
| AACCGACCGGAATCACCGGT+TGG | 0.622675 | 7.3:+41740292 | None:intergenic |
| AGTCTCATGTATGAATATGA+AGG | 0.623950 | 7.3:+41740316 | None:intergenic |
| AGTAGAAGAAGAAACATGGT+TGG | 0.627898 | 7.3:+41740246 | None:intergenic |
| CGATGTGTGAGACAATTATG+TGG | 0.634825 | 7.3:+41740184 | None:intergenic |
| ACAATAATTCAATACAAAGG+TGG | 0.639646 | 7.3:-41740376 | MS.gene89803:CDS |
| AAGACAATAATTCAATACAA+AGG | 0.658842 | 7.3:-41740379 | MS.gene89803:CDS |
| TTGCAGTAGAAGAAGAAACA+TGG | 0.697263 | 7.3:+41740242 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGACAATAATTCAATACAA+AGG | - | chr7.3:41740195-41740214 | MS.gene89803:CDS | 20.0% |
| ! | ACAATAATTCAATACAAAGG+TGG | - | chr7.3:41740198-41740217 | MS.gene89803:CDS | 25.0% |
| ! | ATTATTGTCTTGTAAACCAA+AGG | + | chr7.3:41740186-41740205 | None:intergenic | 25.0% |
| AGTCTCATGTATGAATATGA+AGG | + | chr7.3:41740261-41740280 | None:intergenic | 30.0% | |
| AGTAGAAGAAGAAACATGGT+TGG | + | chr7.3:41740331-41740350 | None:intergenic | 35.0% | |
| CAATACAAAGGTGGTAAAGA+AGG | - | chr7.3:41740207-41740226 | MS.gene89803:CDS | 35.0% | |
| GCACAAATCATTGTTTCATC+TGG | - | chr7.3:41740357-41740376 | MS.gene89803:CDS | 35.0% | |
| TTGCAGTAGAAGAAGAAACA+TGG | + | chr7.3:41740335-41740354 | None:intergenic | 35.0% | |
| AACATGGTTGGAAGATGAAG+TGG | + | chr7.3:41740319-41740338 | None:intergenic | 40.0% | |
| CGATGTGTGAGACAATTATG+TGG | + | chr7.3:41740393-41740412 | None:intergenic | 40.0% | |
| TCATACATGAGACTTCCAAC+CGG | - | chr7.3:41740267-41740286 | MS.gene89803:CDS | 40.0% | |
| !! | AAGAAGGTGCTATAGGATCA+TGG | - | chr7.3:41740223-41740242 | MS.gene89803:CDS | 40.0% |
| !! | AGAAGGTGCTATAGGATCAT+GGG | - | chr7.3:41740224-41740243 | MS.gene89803:CDS | 40.0% |
| ! | GGTTTGCATTTGAAACCGAC+CGG | + | chr7.3:41740298-41740317 | None:intergenic | 45.0% |
| !! | GGTGGTAAAGAAGGTGCTAT+AGG | - | chr7.3:41740216-41740235 | MS.gene89803:CDS | 45.0% |
| AGACTTCCAACCGGTGATTC+CGG | - | chr7.3:41740276-41740295 | MS.gene89803:CDS | 50.0% | |
| TTGAAACCGACCGGAATCAC+CGG | + | chr7.3:41740289-41740308 | None:intergenic | 50.0% | |
| TTCCAACCGGTGATTCCGGT+CGG | - | chr7.3:41740280-41740299 | MS.gene89803:CDS | 55.0% | |
| AACCGACCGGAATCACCGGT+TGG | + | chr7.3:41740285-41740304 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 41740168 | 41740428 | 41740168 | ID=MS.gene89803 |
| chr7.3 | mRNA | 41740168 | 41740428 | 41740168 | ID=MS.gene89803.t1;Parent=MS.gene89803 |
| chr7.3 | exon | 41740168 | 41740428 | 41740168 | ID=MS.gene89803.t1.exon1;Parent=MS.gene89803.t1 |
| chr7.3 | CDS | 41740168 | 41740428 | 41740168 | ID=cds.MS.gene89803.t1;Parent=MS.gene89803.t1 |
| Gene Sequence |
| Protein sequence |
