AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24491


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24491.t1 MTR_1g017830 91.026 156 6 2 1 153 10 160 6.26e-90 259
MS.gene24491.t1 MTR_3g103290 57.516 153 50 5 2 150 13 154 4.43e-48 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24491.t1 AT1G10140 46.753 154 73 5 2 147 8 160 7.18e-23 89.0
MS.gene24491.t1 AT1G58420 43.976 166 75 4 2 150 11 175 4.10e-20 82.4

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAACAGAAATTGAATAA+AGG 0.334932 1.1:-4805206 MS.gene24491:CDS
GCCGCTTCTGCTAGAACTTT+AGG 0.343900 1.1:-4805266 MS.gene24491:CDS
TGTTCACCAATAAGAGCATT+AGG 0.382819 1.1:-4805482 MS.gene24491:CDS
AGCAACAGAAATTGAATAAA+GGG 0.385574 1.1:-4805205 MS.gene24491:CDS
TCTAGTGTTGAAGGAAAAGT+TGG 0.393697 1.1:-4805125 MS.gene24491:CDS
ACAGCATAACTCTTTGATCT+AGG 0.434486 1.1:+4805083 None:intergenic
AAGATCATCAGCATCATTAG+AGG 0.441233 1.1:-4805318 MS.gene24491:CDS
TGTGGTCAAAATATGAGTTA+TGG 0.456641 1.1:-4805422 MS.gene24491:CDS
CCTTCAACACTAGAATCAAA+AGG 0.478362 1.1:+4805134 None:intergenic
TTTAGGTAATAGAATTGATG+TGG 0.523602 1.1:-4805249 MS.gene24491:CDS
AATTTACTAGCAGCATCCAT+TGG 0.526181 1.1:+4805392 None:intergenic
TTACCTAAGTCTAGTAGTGT+TGG 0.528478 1.1:-4805182 MS.gene24491:CDS
ACCTAAAGTTCTAGCAGAAG+CGG 0.542651 1.1:+4805265 None:intergenic
ATTCCAACACTACTAGACTT+AGG 0.543949 1.1:+4805179 None:intergenic
TAAGTCTAGTAGTGTTGGAA+TGG 0.550381 1.1:-4805177 MS.gene24491:CDS
AGATCAAAGAGTTATGCTGT+TGG 0.581741 1.1:-4805080 MS.gene24491:CDS
GAGTTATGGAAGTTATCCAA+TGG 0.620886 1.1:-4805408 MS.gene24491:CDS
AGATCATCAGCATCATTAGA+GGG 0.626119 1.1:-4805317 MS.gene24491:CDS
AGAACCAAAACATGTTTGTG+AGG 0.637515 1.1:-4805511 None:intergenic
TGGAATGGCTAAGATTGATG+AGG 0.704946 1.1:-4805162 MS.gene24491:CDS
GTAAGAAGCATAACAAACTG+TGG 0.710541 1.1:-4805440 MS.gene24491:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCAACAGAAATTGAATAAA+GGG - chr1.1:4805347-4805366 MS.gene24491:CDS 25.0%
! TTTAGGTAATAGAATTGATG+TGG - chr1.1:4805303-4805322 MS.gene24491:CDS 25.0%
CAGCAACAGAAATTGAATAA+AGG - chr1.1:4805346-4805365 MS.gene24491:CDS 30.0%
TGTGGTCAAAATATGAGTTA+TGG - chr1.1:4805130-4805149 MS.gene24491:CDS 30.0%
!! AAGATTTTGTTGAGCTTGTT+AGG - chr1.1:4805263-4805282 MS.gene24491:CDS 30.0%
!!! AGATTTTGTTGAGCTTGTTA+GGG - chr1.1:4805264-4805283 MS.gene24491:CDS 30.0%
AAGATCATCAGCATCATTAG+AGG - chr1.1:4805234-4805253 MS.gene24491:CDS 35.0%
AATTTACTAGCAGCATCCAT+TGG + chr1.1:4805163-4805182 None:intergenic 35.0%
ACAGCATAACTCTTTGATCT+AGG + chr1.1:4805472-4805491 None:intergenic 35.0%
AGATCAAAGAGTTATGCTGT+TGG - chr1.1:4805472-4805491 MS.gene24491:CDS 35.0%
AGATCATCAGCATCATTAGA+GGG - chr1.1:4805235-4805254 MS.gene24491:CDS 35.0%
ATTCCAACACTACTAGACTT+AGG + chr1.1:4805376-4805395 None:intergenic 35.0%
CCTTCAACACTAGAATCAAA+AGG + chr1.1:4805421-4805440 None:intergenic 35.0%
GAGTTATGGAAGTTATCCAA+TGG - chr1.1:4805144-4805163 MS.gene24491:CDS 35.0%
TTACCTAAGTCTAGTAGTGT+TGG - chr1.1:4805370-4805389 MS.gene24491:CDS 35.0%
! GCTTTTCCTAATGCTCTTAT+TGG + chr1.1:4805079-4805098 None:intergenic 35.0%
! GTAAGAAGCATAACAAACTG+TGG - chr1.1:4805112-4805131 MS.gene24491:CDS 35.0%
! TAAGTCTAGTAGTGTTGGAA+TGG - chr1.1:4805375-4805394 MS.gene24491:CDS 35.0%
! TCTAGTGTTGAAGGAAAAGT+TGG - chr1.1:4805427-4805446 MS.gene24491:CDS 35.0%
!! CCTTTTGATTCTAGTGTTGA+AGG - chr1.1:4805418-4805437 MS.gene24491:CDS 35.0%
!! TGTTCACCAATAAGAGCATT+AGG - chr1.1:4805070-4805089 MS.gene24491:CDS 35.0%
TGGAATGGCTAAGATTGATG+AGG - chr1.1:4805390-4805409 MS.gene24491:CDS 40.0%
! ACCTAAAGTTCTAGCAGAAG+CGG + chr1.1:4805290-4805309 None:intergenic 40.0%
GCCGCTTCTGCTAGAACTTT+AGG - chr1.1:4805286-4805305 MS.gene24491:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 4805052 4805522 4805052 ID=MS.gene24491
chr1.1 mRNA 4805052 4805522 4805052 ID=MS.gene24491.t1;Parent=MS.gene24491
chr1.1 exon 4805052 4805522 4805052 ID=MS.gene24491.t1.exon1;Parent=MS.gene24491.t1
chr1.1 CDS 4805052 4805522 4805052 ID=cds.MS.gene24491.t1;Parent=MS.gene24491.t1
Gene Sequence

>MS.gene24491

ATGTTTGTGAGGATCATGTGTTCACCAATAAGAGCATTAGGAAAAGCAAGAGATGCATACGTAAGAAGCATAACAAACTGTGGTCAAAATATGAGTTATGGAAGTTATCCAATGGATGCTGCTAGTAAATTTTCTTTATCAAGAAGTCAAAGTGCAGCAACATCAAGAAGATCATCATCAAGAAGATCATCAGCATCATTAGAGGGTAATGAAGATTTTGTTGAGCTTGTTAGGGCCGCTTCTGCTAGAACTTTAGGTAATAGAATTGATGTGGATTTAGTTCTCAAGCAACAACAGCAACAGAAATTGAATAAAGGGTTACCTAAGTCTAGTAGTGTTGGAATGGCTAAGATTGATGAGGATATGCCTTTTGATTCTAGTGTTGAAGGAAAAGTTGGTTTTGTTGCTGATTCTTATCCTAGATCAAAGAGTTATGCTGTTGGACATAGAAGAAATGTTGTTGCTTTCTGA

Protein sequence

>MS.gene24491.t1

MFVRIMCSPIRALGKARDAYVRSITNCGQNMSYGSYPMDAASKFSLSRSQSAATSRRSSSRRSSASLEGNEDFVELVRAASARTLGNRIDVDLVLKQQQQQKLNKGLPKSSSVGMAKIDEDMPFDSSVEGKVGFVADSYPRSKSYAVGHRRNVVAF