Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89320.t1 | AFK47532.1 | 93 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 7.70E-38 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89320.t1 | I3T4U0 | 93.0 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 5.5e-38 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049611 | MS.gene89320 | 0.816147 | 6.42E-52 | -1.69E-46 |
| MS.gene049634 | MS.gene89320 | 0.813002 | 3.19E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene89320 | -0.827496 | 1.52E-54 | -1.69E-46 |
| MS.gene050380 | MS.gene89320 | 0.829304 | 5.55E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene89320 | 0.842369 | 2.70E-58 | -1.69E-46 |
| MS.gene050647 | MS.gene89320 | 0.801061 | 1.08E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene89320 | -0.825859 | 3.73E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene89320 | -0.826312 | 2.91E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene89320 | 0.817597 | 3.04E-52 | -1.69E-46 |
| MS.gene051138 | MS.gene89320 | 0.806407 | 8.35E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene89320 | -0.817387 | 3.39E-52 | -1.69E-46 |
| MS.gene051874 | MS.gene89320 | -0.821209 | 4.56E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene89320 | 0.802564 | 5.29E-49 | -1.69E-46 |
| MS.gene05234 | MS.gene89320 | 0.800638 | 1.31E-48 | -1.69E-46 |
| MS.gene052966 | MS.gene89320 | -0.81535 | 9.67E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene89320 | 0.84974 | 2.66E-60 | -1.69E-46 |
| MS.gene053561 | MS.gene89320 | 0.833243 | 5.96E-56 | -1.69E-46 |
| MS.gene053562 | MS.gene89320 | 0.807748 | 4.34E-50 | -1.69E-46 |
| MS.gene054290 | MS.gene89320 | 0.805554 | 1.26E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene89320 | 0.803727 | 3.04E-49 | -1.69E-46 |
| MS.gene054708 | MS.gene89320 | 0.829679 | 4.50E-55 | -1.69E-46 |
| MS.gene055124 | MS.gene89320 | 0.841902 | 3.58E-58 | -1.69E-46 |
| MS.gene055139 | MS.gene89320 | 0.80632 | 8.71E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene89320 | 0.831238 | 1.87E-55 | -1.69E-46 |
| MS.gene055202 | MS.gene89320 | 0.935942 | 3.83E-97 | -1.69E-46 |
| MS.gene05589 | MS.gene89320 | -0.801278 | 9.71E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene89320 | 0.803297 | 3.73E-49 | -1.69E-46 |
| MS.gene056313 | MS.gene89320 | 0.81468 | 1.36E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene89320 | -0.812299 | 4.55E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene89320 | -0.801289 | 9.66E-49 | -1.69E-46 |
| MS.gene056880 | MS.gene89320 | 0.808709 | 2.71E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene89320 | 0.803465 | 3.44E-49 | -1.69E-46 |
| MS.gene057473 | MS.gene89320 | 0.814073 | 1.85E-51 | -1.69E-46 |
| MS.gene058093 | MS.gene89320 | 0.806432 | 8.25E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene89320 | 0.808358 | 3.22E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene89320 | 0.849664 | 2.80E-60 | -1.69E-46 |
| MS.gene058173 | MS.gene89320 | 0.84165 | 4.18E-58 | -1.69E-46 |
| MS.gene058175 | MS.gene89320 | 0.851953 | 6.34E-61 | -1.69E-46 |
| MS.gene058840 | MS.gene89320 | 0.828389 | 9.24E-55 | -1.69E-46 |
| MS.gene059037 | MS.gene89320 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene89320 | 0.807065 | 6.06E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene89320 | -0.805377 | 1.37E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene89320 | -0.817622 | 3.00E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene89320 | -0.800605 | 1.33E-48 | -1.69E-46 |
| MS.gene060595 | MS.gene89320 | 0.817795 | 2.74E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene89320 | -0.80049 | 1.41E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89320.t1 | MTR_7g063490 | 93.023 | 86 | 6 | 0 | 1 | 86 | 1 | 86 | 1.05e-46 | 144 |
| MS.gene89320.t1 | MTR_4g021330 | 55.056 | 89 | 36 | 3 | 1 | 85 | 1 | 89 | 1.31e-23 | 85.9 |
| MS.gene89320.t1 | MTR_2g104750 | 50.000 | 86 | 38 | 4 | 1 | 81 | 1 | 86 | 3.85e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACTTCCAACCGGTGATTC+CGG | 0.332515 | 7.1:-38170442 | MS.gene89320:CDS |
| AATGGAGGGTCTTATACCTT+TGG | 0.361131 | 7.1:-38170551 | MS.gene89320:CDS |
| GCACAAATCATTGTTTCATC+TGG | 0.387174 | 7.1:-38170361 | MS.gene89320:CDS |
| GGTGGTAAAGAAGGTGCTAT+AGG | 0.442411 | 7.1:-38170502 | MS.gene89320:CDS |
| AAGAAGGTGCTATAGGATCA+TGG | 0.480205 | 7.1:-38170495 | MS.gene89320:CDS |
| TTGAAACCGACCGGAATCAC+CGG | 0.491223 | 7.1:+38170432 | None:intergenic |
| CAATACAAAGGTGGTAAAGA+AGG | 0.512508 | 7.1:-38170511 | MS.gene89320:CDS |
| AACATGGTTGGAAGATGAAG+TGG | 0.530962 | 7.1:+38170402 | None:intergenic |
| ATTATTGTCTTGTAAACCAA+AGG | 0.536810 | 7.1:+38170535 | None:intergenic |
| AGAAGGTGCTATAGGATCAT+GGG | 0.557929 | 7.1:-38170494 | MS.gene89320:CDS |
| GGTTTGCATTTGAAACCGAC+CGG | 0.571028 | 7.1:+38170423 | None:intergenic |
| TCATACATGAGACTTCCAAC+CGG | 0.604318 | 7.1:-38170451 | MS.gene89320:CDS |
| TTCCAACCGGTGATTCCGGT+CGG | 0.604554 | 7.1:-38170438 | MS.gene89320:CDS |
| AACCGACCGGAATCACCGGT+TGG | 0.622675 | 7.1:+38170436 | None:intergenic |
| AGTAGAAGAAGAAACATGGT+TGG | 0.627898 | 7.1:+38170390 | None:intergenic |
| CGATGTGTGAGACAATTATG+TGG | 0.634825 | 7.1:+38170328 | None:intergenic |
| ACAATAATTCAATACAAAGG+TGG | 0.639646 | 7.1:-38170520 | MS.gene89320:CDS |
| AAGACAATAATTCAATACAA+AGG | 0.658842 | 7.1:-38170523 | MS.gene89320:CDS |
| AGTCTCATGTATGAATACGA+AGG | 0.695808 | 7.1:+38170460 | None:intergenic |
| TTGCAGTAGAAGAAGAAACA+TGG | 0.697263 | 7.1:+38170386 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGACAATAATTCAATACAA+AGG | - | chr7.1:38170339-38170358 | MS.gene89320:CDS | 20.0% |
| ! | ACAATAATTCAATACAAAGG+TGG | - | chr7.1:38170342-38170361 | MS.gene89320:CDS | 25.0% |
| ! | ATTATTGTCTTGTAAACCAA+AGG | + | chr7.1:38170330-38170349 | None:intergenic | 25.0% |
| AGTAGAAGAAGAAACATGGT+TGG | + | chr7.1:38170475-38170494 | None:intergenic | 35.0% | |
| AGTCTCATGTATGAATACGA+AGG | + | chr7.1:38170405-38170424 | None:intergenic | 35.0% | |
| CAATACAAAGGTGGTAAAGA+AGG | - | chr7.1:38170351-38170370 | MS.gene89320:CDS | 35.0% | |
| GCACAAATCATTGTTTCATC+TGG | - | chr7.1:38170501-38170520 | MS.gene89320:CDS | 35.0% | |
| TTGCAGTAGAAGAAGAAACA+TGG | + | chr7.1:38170479-38170498 | None:intergenic | 35.0% | |
| AACATGGTTGGAAGATGAAG+TGG | + | chr7.1:38170463-38170482 | None:intergenic | 40.0% | |
| CGATGTGTGAGACAATTATG+TGG | + | chr7.1:38170537-38170556 | None:intergenic | 40.0% | |
| TCATACATGAGACTTCCAAC+CGG | - | chr7.1:38170411-38170430 | MS.gene89320:CDS | 40.0% | |
| !! | AAGAAGGTGCTATAGGATCA+TGG | - | chr7.1:38170367-38170386 | MS.gene89320:CDS | 40.0% |
| !! | AGAAGGTGCTATAGGATCAT+GGG | - | chr7.1:38170368-38170387 | MS.gene89320:CDS | 40.0% |
| ! | GGTTTGCATTTGAAACCGAC+CGG | + | chr7.1:38170442-38170461 | None:intergenic | 45.0% |
| !! | GGTGGTAAAGAAGGTGCTAT+AGG | - | chr7.1:38170360-38170379 | MS.gene89320:CDS | 45.0% |
| AGACTTCCAACCGGTGATTC+CGG | - | chr7.1:38170420-38170439 | MS.gene89320:CDS | 50.0% | |
| TTGAAACCGACCGGAATCAC+CGG | + | chr7.1:38170433-38170452 | None:intergenic | 50.0% | |
| TTCCAACCGGTGATTCCGGT+CGG | - | chr7.1:38170424-38170443 | MS.gene89320:CDS | 55.0% | |
| AACCGACCGGAATCACCGGT+TGG | + | chr7.1:38170429-38170448 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 38170312 | 38170572 | 38170312 | ID=MS.gene89320 |
| chr7.1 | mRNA | 38170312 | 38170572 | 38170312 | ID=MS.gene89320.t1;Parent=MS.gene89320 |
| chr7.1 | exon | 38170312 | 38170572 | 38170312 | ID=MS.gene89320.t1.exon1;Parent=MS.gene89320.t1 |
| chr7.1 | CDS | 38170312 | 38170572 | 38170312 | ID=cds.MS.gene89320.t1;Parent=MS.gene89320.t1 |
| Gene Sequence |
| Protein sequence |
