AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCATTGGTATCGGTAATT+TGG 0.233892 2.3:-67488932 None:intergenic
TTAGGTGGATTGACGCTAAA+TGG 0.269497 2.3:+67491722 MS.gene05841:CDS
TATAATTTACATACCATAAA+AGG 0.293975 2.3:-67489973 None:intergenic
TCCTGGTTGCTTCCTCTTTG+TGG 0.305566 2.3:-67491661 None:intergenic
TCAGTTCATCACCCTGAGTT+TGG 0.330540 2.3:+67490573 MS.gene05841:CDS
TCCTCCAGGCTATAAAATTA+TGG 0.331657 2.3:+67491536 MS.gene05841:intron
AGTACCTTCGTAGAGAGATA+TGG 0.376715 2.3:-67488787 None:intergenic
GTAATTTGGCGAGCTTCATC+TGG 0.388533 2.3:-67488918 None:intergenic
GTCCTAGAGACAGGTGGTTT+AGG 0.396339 2.3:+67491704 MS.gene05841:CDS
AATAAGAGTGGTGAAGGAAA+TGG 0.409411 2.3:-67488676 None:intergenic
AGGTTGCTGTATGGATATAC+TGG 0.426939 2.3:+67490685 MS.gene05841:CDS
GTAAAATAGTTTCCAAACTC+AGG 0.442321 2.3:-67490585 None:intergenic
TATGGGTTCAGCTCCGTCAT+TGG 0.448545 2.3:-67488769 None:intergenic
GCGCGTGAGGTGCACGGTCG+AGG 0.452719 2.3:-67488878 None:intergenic
TGATGTCGAGCACAACTTGT+TGG 0.459330 2.3:-67490622 None:intergenic
CGCCGCAGTAGCGCGTGAAA+AGG 0.462406 2.3:-67488714 None:intergenic
AATATCAAAGCAAGTGATCC+TGG 0.464370 2.3:+67490528 MS.gene05841:CDS
TGTTCATATCCCACACTAGC+TGG 0.466991 2.3:-67489114 None:intergenic
TTCATCTGGAGAAAGATGAT+TGG 0.468451 2.3:-67488904 None:intergenic
GAACCGGGTGGCGGCGCTGA+TGG 0.470400 2.3:-67488815 None:intergenic
TACGTGGGAATGGCATATCT+AGG 0.480461 2.3:-67491586 None:intergenic
CTTCGTCAGCTAAATAATCC+TGG 0.483547 2.3:-67491678 None:intergenic
AACTATGACAGGATATGCTT+GGG 0.484809 2.3:+67490499 MS.gene05841:intron
TAGCAATAATACACACTCAA+TGG 0.488999 2.3:-67489141 None:intergenic
AGGACTGACATGCAAAGCTT+TGG 0.490500 2.3:-67489953 None:intergenic
TTCGTCCCTGTACGTGGGAA+TGG 0.491251 2.3:-67491596 None:intergenic
ATCCATACAGCAACCTTATG+AGG 0.491264 2.3:-67490678 None:intergenic
TATTGAAAGGAATGGTGAAC+CGG 0.494360 2.3:-67488831 None:intergenic
AGAATGCGGATTGAAAATAA+AGG 0.498652 2.3:-67489920 None:intergenic
TCATCTGGAGAAAGATGATT+GGG 0.500771 2.3:-67488903 None:intergenic
CTACCATCAGCGCCGCCACC+CGG 0.504605 2.3:+67488812 MS.gene05841:CDS
GAACTATGACAGGATATGCT+TGG 0.507027 2.3:+67490498 MS.gene05841:intron
GATATGCCATTCCCACGTAC+AGG 0.511186 2.3:+67491590 MS.gene05841:CDS
GACAGGATATGCTTGGGAGT+TGG 0.511416 2.3:+67490505 MS.gene05841:intron
TTCAGCTCCGTCATTGGAAG+AGG 0.511539 2.3:-67488763 None:intergenic
GTACCTTCGTAGAGAGATAT+GGG 0.518661 2.3:-67488786 None:intergenic
AAACACTTACATGGGTCCAT+TGG 0.525548 2.3:-67488946 None:intergenic
AGGAATGGTGAACCGGGTGG+CGG 0.528502 2.3:-67488824 None:intergenic
GCTTCTTCCTCTTCCAATGA+CGG 0.530349 2.3:+67488756 MS.gene05841:CDS
GGATAGAATAAGAGTGGTGA+AGG 0.535689 2.3:-67488682 None:intergenic
AAGGTTACTCTTTCAGCCCA+TGG 0.536237 2.3:-67489901 None:intergenic
CTAGAGACAGGTGGTTTAGG+TGG 0.538191 2.3:+67491707 MS.gene05841:CDS
ATTGACAATTCCAGCTAGTG+TGG 0.542422 2.3:+67489104 MS.gene05841:CDS
ACGAAGCAAGTCCTAGAGAC+AGG 0.547793 2.3:+67491695 MS.gene05841:CDS
CACCTAAACCACCTGTCTCT+AGG 0.548815 2.3:-67491706 None:intergenic
CGCGTGAAAAGGAAGGATAA+TGG 0.557879 2.3:-67488703 None:intergenic
GATAGAAAACTGCAGTGTTG+TGG 0.563843 2.3:+67491631 MS.gene05841:CDS
TAAAGCTTCGTCCCTGTACG+TGG 0.567298 2.3:-67491602 None:intergenic
GATAATGGATAGAATAAGAG+TGG 0.572586 2.3:-67488688 None:intergenic
TGCCTCATAAGGTTGCTGTA+TGG 0.575098 2.3:+67490676 MS.gene05841:CDS
TAAAATAGTTTCCAAACTCA+GGG 0.579999 2.3:-67490584 None:intergenic
ACGTGGGAATGGCATATCTA+GGG 0.589402 2.3:-67491585 None:intergenic
AGAGGAAGAAGCGGCGCGTG+AGG 0.594894 2.3:-67488745 None:intergenic
GTAGCGAACATTGTATTGAA+AGG 0.600684 2.3:-67488844 None:intergenic
TCCACAAAGAGGAAGCAACC+AGG 0.603581 2.3:+67491660 MS.gene05841:CDS
TTACATGGGTCCATTGGTAT+CGG 0.607772 2.3:-67488940 None:intergenic
CGCCAAATTACCGATACCAA+TGG 0.613455 2.3:+67488930 MS.gene05841:CDS
GGATTGACGCTAAATGGCCA+TGG 0.618793 2.3:+67491728 MS.gene05841:CDS
GAACATTGTATTGAAAGGAA+TGG 0.618795 2.3:-67488839 None:intergenic
TGGGTGGCGCGTGAGGTGCA+CGG 0.621460 2.3:-67488884 None:intergenic
AGATGATTGGGTGGCGCGTG+AGG 0.621550 2.3:-67488891 None:intergenic
GCAGTAGCGCGTGAAAAGGA+AGG 0.621678 2.3:-67488710 None:intergenic
TCCAGGCTATAAAATTATGG+TGG 0.628267 2.3:+67491539 MS.gene05841:intron
GTCATTGGAAGAGGAAGAAG+CGG 0.628278 2.3:-67488754 None:intergenic
GTTGTGCTCGACATCAGCAT+CGG 0.629113 2.3:+67490629 MS.gene05841:CDS
ATATGCCATTCCCACGTACA+GGG 0.629309 2.3:+67491591 MS.gene05841:CDS
TCAGGTAACAAATACACCAT+GGG 0.642717 2.3:+67489885 MS.gene05841:intron
TTTGAAGAAGTGCATTGCTG+AGG 0.643953 2.3:+67489188 MS.gene05841:CDS
TTCAGGTAACAAATACACCA+TGG 0.647309 2.3:+67489884 MS.gene05841:intron
TTGACAATTCCAGCTAGTGT+GGG 0.653320 2.3:+67489105 MS.gene05841:CDS
TCTGGAGAAAGATGATTGGG+TGG 0.677082 2.3:-67488900 None:intergenic
ATTGAAAGGAATGGTGAACC+GGG 0.679558 2.3:-67488830 None:intergenic
TGTGGATTCAGTCCACAAAG+AGG 0.695546 2.3:+67491649 MS.gene05841:CDS
GAAAGGAATGGTGAACCGGG+TGG 0.699357 2.3:-67488827 None:intergenic
AAAGCTTCGTCCCTGTACGT+GGG 0.700795 2.3:-67491601 None:intergenic
TTGGCTACTAGATCAGAATG+CGG 0.714234 2.3:-67489934 None:intergenic
AAGCAAGTCCTAGAGACAGG+TGG 0.718600 2.3:+67491698 MS.gene05841:CDS
GAACCCATATCTCTCTACGA+AGG 0.724309 2.3:+67488783 MS.gene05841:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGACAATTAATAAAA+GGG + chr2.3:67490242-67490261 MS.gene05841:intron 10.0%
!! AATACTTAATCATTTAAAAA+TGG - chr2.3:67490722-67490741 None:intergenic 10.0%
!! TAAAAATGACAATTAATAAA+AGG + chr2.3:67490241-67490260 MS.gene05841:intron 10.0%
!! ATTATATGTGTAAATTGAAA+AGG - chr2.3:67489252-67489271 None:intergenic 15.0%
!! CATATAAAACATTGTTTATA+TGG + chr2.3:67491162-67491181 MS.gene05841:intron 15.0%
!! TATAATTTACATACCATAAA+AGG - chr2.3:67489976-67489995 None:intergenic 15.0%
!!! TTAATTTAAGTTAACTATGT+AGG - chr2.3:67490219-67490238 None:intergenic 15.0%
!!! TTAGTTATTTTGAAAATTGA+GGG - chr2.3:67490975-67490994 None:intergenic 15.0%
!! CAAAAAATATTTACTTCTCA+TGG - chr2.3:67490836-67490855 None:intergenic 20.0%
!! TGAGAAGATTAATTAATTAG+TGG + chr2.3:67489338-67489357 MS.gene05841:intron 20.0%
!! TTACGTTTCAATTTAAATGT+AGG + chr2.3:67489028-67489047 MS.gene05841:intron 20.0%
!! TTCTATCATAAACCATATTT+GGG + chr2.3:67489572-67489591 MS.gene05841:intron 20.0%
!! TTTCAATTCAAATGCAAAAA+TGG - chr2.3:67491392-67491411 None:intergenic 20.0%
!!! AAATATTTTTTGCTTGAGAT+TGG + chr2.3:67490844-67490863 MS.gene05841:intron 20.0%
!!! AATATTTTTTGCTTGAGATT+GGG + chr2.3:67490845-67490864 MS.gene05841:intron 20.0%
!!! ATTCTTTACTTTTTCTCAAT+TGG + chr2.3:67488975-67488994 MS.gene05841:intron 20.0%
!!! CAGAAAAGCTATTTTTATTA+AGG - chr2.3:67490446-67490465 None:intergenic 20.0%
!!! GTTAGTTATTTTGAAAATTG+AGG - chr2.3:67490976-67490995 None:intergenic 20.0%
!!! TATTTTTAAATCCACGATTT+TGG + chr2.3:67490948-67490967 MS.gene05841:intron 20.0%
!!! TGTAGCTTTTGAATAAATAT+TGG - chr2.3:67490337-67490356 None:intergenic 20.0%
!!! TTCTTTACTTTTTCTCAATT+GGG + chr2.3:67488976-67488995 MS.gene05841:intron 20.0%
!!! TTGTTATGATGAATTTTCAT+TGG + chr2.3:67490763-67490782 MS.gene05841:intron 20.0%
!!! TTTGACTTAAAATGCATTTT+TGG + chr2.3:67490900-67490919 MS.gene05841:intron 20.0%
! AAAATAAAGGCAGTAAAGTT+GGG - chr2.3:67491422-67491441 None:intergenic 25.0%
! AAACTATAAGAGAAACTGTT+AGG - chr2.3:67489624-67489643 None:intergenic 25.0%
! AATGAAGAAAAACACTTACA+TGG - chr2.3:67488958-67488977 None:intergenic 25.0%
! AATTCAGGGATCTAAAAATA+TGG + chr2.3:67491310-67491329 MS.gene05841:intron 25.0%
! AGAAGAATAAACTGACAAAA+TGG - chr2.3:67489680-67489699 None:intergenic 25.0%
! AGATTAATTAATTAGTGGTG+AGG + chr2.3:67489343-67489362 MS.gene05841:intron 25.0%
! ATGAAGAAAAACACTTACAT+GGG - chr2.3:67488957-67488976 None:intergenic 25.0%
! ATTCAGGGATCTAAAAATAT+GGG + chr2.3:67491311-67491330 MS.gene05841:intron 25.0%
! CAACATGTCCATTAAAATAA+AGG - chr2.3:67491435-67491454 None:intergenic 25.0%
! CATATGGAGAAAATTCAATT+GGG + chr2.3:67489423-67489442 MS.gene05841:intron 25.0%
! GATTAATTAATTAGTGGTGA+GGG + chr2.3:67489344-67489363 MS.gene05841:intron 25.0%
! GTCAGTTTATTCTTCTTTAT+AGG + chr2.3:67489684-67489703 MS.gene05841:intron 25.0%
! GTTCTATCATAAACCATATT+TGG + chr2.3:67489571-67489590 MS.gene05841:intron 25.0%
! TAAAATAAAGGCAGTAAAGT+TGG - chr2.3:67491423-67491442 None:intergenic 25.0%
! TAAAATAGTTTCCAAACTCA+GGG - chr2.3:67490587-67490606 None:intergenic 25.0%
! TCTGTGACATAATACTATTT+AGG + chr2.3:67491069-67491088 MS.gene05841:intron 25.0%
! TTGTTAGGTTATCATGTTAT+AGG - chr2.3:67489484-67489503 None:intergenic 25.0%
!! AGCAATTTAAAATCTCACTT+CGG + chr2.3:67490788-67490807 MS.gene05841:intron 25.0%
!! ATTCAGATACTTGATAATTC+AGG + chr2.3:67489774-67489793 MS.gene05841:intron 25.0%
!! CGTAAAAATCAAATTTTGCA+TGG - chr2.3:67489013-67489032 None:intergenic 25.0%
!! GCTTTTGAATAAATATTGGT+TGG - chr2.3:67490333-67490352 None:intergenic 25.0%
!! GTTTCTCTTATAGTTTTTCT+AGG + chr2.3:67489628-67489647 MS.gene05841:intron 25.0%
!! TATTTTGTCTCATACGATTT+TGG - chr2.3:67491338-67491357 None:intergenic 25.0%
!!! ATAGCTTTTCTGCATTTTTT+CGG + chr2.3:67490454-67490473 MS.gene05841:intron 25.0%
!!! CGATGTTTTTTTTTTGTTTC+AGG + chr2.3:67489867-67489886 MS.gene05841:intron 25.0%
!!! CTTTACTGCCTTTATTTTAA+TGG + chr2.3:67491424-67491443 MS.gene05841:intron 25.0%
!!! TATTTTTAGATCCCTGAATT+TGG - chr2.3:67491310-67491329 None:intergenic 25.0%
AAAATCAACCCAAATGCATA+TGG + chr2.3:67491258-67491277 MS.gene05841:intron 30.0%
AAATCAACCCAAATGCATAT+GGG + chr2.3:67491259-67491278 MS.gene05841:intron 30.0%
AATTGAAAACATGTCCAAAG+AGG - chr2.3:67491476-67491495 None:intergenic 30.0%
ATGTAGCTATCTCATTTAGT+AGG - chr2.3:67489531-67489550 None:intergenic 30.0%
CAACAACAACAACAAAATAG+AGG - chr2.3:67490928-67490947 None:intergenic 30.0%
CAATGAAAAGTTACTACTCT+TGG - chr2.3:67490306-67490325 None:intergenic 30.0%
CTTCTTTATAGGATTTCTCT+TGG + chr2.3:67489695-67489714 MS.gene05841:intron 30.0%
GAACATTGTATTGAAAGGAA+TGG - chr2.3:67488842-67488861 None:intergenic 30.0%
GAAGAGGAAAACACAATATA+TGG - chr2.3:67490106-67490125 None:intergenic 30.0%
GCATAATATGATTCAAACTG+AGG + chr2.3:67490137-67490156 MS.gene05841:intron 30.0%
GCATATGGAGAAAATTCAAT+TGG + chr2.3:67489422-67489441 MS.gene05841:intron 30.0%
GTAAAATAGTTTCCAAACTC+AGG - chr2.3:67490588-67490607 None:intergenic 30.0%
TAATTGTGTGAGAACTAAGA+TGG - chr2.3:67491218-67491237 None:intergenic 30.0%
TACTAAATGAGATAGCTACA+TGG + chr2.3:67489530-67489549 MS.gene05841:intron 30.0%
TAGCAATAATACACACTCAA+TGG - chr2.3:67489144-67489163 None:intergenic 30.0%
TCCTAAAATCTGCATAGAAA+TGG - chr2.3:67489999-67490018 None:intergenic 30.0%
TTTGTCGCTAAACATAGTAA+GGG + chr2.3:67489303-67489322 MS.gene05841:intron 30.0%
! ACCATTTCTATGCAGATTTT+AGG + chr2.3:67489995-67490014 MS.gene05841:intron 30.0%
! AGAATGCGGATTGAAAATAA+AGG - chr2.3:67489923-67489942 None:intergenic 30.0%
! ATTGTGTTTTCCTCTTCTTT+TGG + chr2.3:67490109-67490128 MS.gene05841:intron 30.0%
! CAATTTAAAATCTCACTTCG+GGG + chr2.3:67490790-67490809 MS.gene05841:intron 30.0%
! GCAATTTAAAATCTCACTTC+GGG + chr2.3:67490789-67490808 MS.gene05841:intron 30.0%
!! AAGTAGAGTATTGTGAAAAC+TGG + chr2.3:67489841-67489860 MS.gene05841:intron 30.0%
!! GATAATGGATAGAATAAGAG+TGG - chr2.3:67488691-67488710 None:intergenic 30.0%
!! GGTGTTTTTCACTTTTATGT+GGG + chr2.3:67489738-67489757 MS.gene05841:intron 30.0%
!! TGGTGTTTTTCACTTTTATG+TGG + chr2.3:67489737-67489756 MS.gene05841:intron 30.0%
!!! TGGGTTGATTTTTGATGATT+TGG - chr2.3:67491250-67491269 None:intergenic 30.0%
!!! TGGTTGTTTTTGTTGTTGTT+AGG - chr2.3:67489499-67489518 None:intergenic 30.0%
AAAACCGAATGACCAAATTC+AGG + chr2.3:67491295-67491314 MS.gene05841:intron 35.0%
AAACCGAATGACCAAATTCA+GGG + chr2.3:67491296-67491315 MS.gene05841:intron 35.0%
AACTATGACAGGATATGCTT+GGG + chr2.3:67490499-67490518 MS.gene05841:intron 35.0%
AATAAGAGTGGTGAAGGAAA+TGG - chr2.3:67488679-67488698 None:intergenic 35.0%
AATATCAAAGCAAGTGATCC+TGG + chr2.3:67490528-67490547 MS.gene05841:CDS 35.0%
AGCTATCTCATTTAGTAGGA+TGG - chr2.3:67489527-67489546 None:intergenic 35.0%
AGCTTCAAAATGTGTAACTG+AGG - chr2.3:67491004-67491023 None:intergenic 35.0%
ATGGAGAAAATTCAATTGGG+AGG + chr2.3:67489426-67489445 MS.gene05841:intron 35.0%
CAACACATACATATCATGGT+TGG + chr2.3:67490179-67490198 MS.gene05841:intron 35.0%
CAACCAACACATACATATCA+TGG + chr2.3:67490175-67490194 MS.gene05841:intron 35.0%
CAACCATGATATGTATGTGT+TGG - chr2.3:67490181-67490200 None:intergenic 35.0%
CTTCTCATGGAAGAAGATAA+TGG - chr2.3:67490823-67490842 None:intergenic 35.0%
CTTTGTCGCTAAACATAGTA+AGG + chr2.3:67489302-67489321 MS.gene05841:intron 35.0%
GAAATACCAGAACAACACTT+TGG + chr2.3:67490867-67490886 MS.gene05841:intron 35.0%
GCTTCAAAATGTGTAACTGA+GGG - chr2.3:67491003-67491022 None:intergenic 35.0%
GTAGCGAACATTGTATTGAA+AGG - chr2.3:67488847-67488866 None:intergenic 35.0%
GTGCAATATTGCACATGTTA+AGG + chr2.3:67490277-67490296 MS.gene05841:intron 35.0%
GTTGAGTGAAAAACAGTTTG+TGG + chr2.3:67489717-67489736 MS.gene05841:intron 35.0%
TATGCAATGACCAAAAGAAG+AGG - chr2.3:67490122-67490141 None:intergenic 35.0%
TATTGAAAGGAATGGTGAAC+CGG - chr2.3:67488834-67488853 None:intergenic 35.0%
TCAGGTAACAAATACACCAT+GGG + chr2.3:67489885-67489904 MS.gene05841:intron 35.0%
TCATCTGGAGAAAGATGATT+GGG - chr2.3:67488906-67488925 None:intergenic 35.0%
TCCAGGCTATAAAATTATGG+TGG + chr2.3:67491539-67491558 MS.gene05841:intron 35.0%
TCCTCCAGGCTATAAAATTA+TGG + chr2.3:67491536-67491555 MS.gene05841:intron 35.0%
TGGAGAAAATTCAATTGGGA+GGG + chr2.3:67489427-67489446 MS.gene05841:intron 35.0%
TTCAGGTAACAAATACACCA+TGG + chr2.3:67489884-67489903 MS.gene05841:intron 35.0%
TTCATCTGGAGAAAGATGAT+TGG - chr2.3:67488907-67488926 None:intergenic 35.0%
TTCTCCATATGCAATAGTCA+GGG - chr2.3:67489414-67489433 None:intergenic 35.0%
TTTCTCCATATGCAATAGTC+AGG - chr2.3:67489415-67489434 None:intergenic 35.0%
! ACCATAATTTTATAGCCTGG+AGG - chr2.3:67491540-67491559 None:intergenic 35.0%
! CAATGAGTTGAACCCAAATA+TGG - chr2.3:67489587-67489606 None:intergenic 35.0%
! GATATGCTAAGATTTTCTCC+AGG - chr2.3:67490549-67490568 None:intergenic 35.0%
! TCCACCATAATTTTATAGCC+TGG - chr2.3:67491543-67491562 None:intergenic 35.0%
! TTTAATGGACATGTTGCCTT+TGG + chr2.3:67491439-67491458 MS.gene05841:intron 35.0%
! TTTGCATGTCAGTCCTTTTA+TGG + chr2.3:67489960-67489979 MS.gene05841:CDS 35.0%
!! TCAAATTTTGCATGGTTCTG+AGG - chr2.3:67489005-67489024 None:intergenic 35.0%
!!! CAAATTTTGCATGGTTCTGA+GGG - chr2.3:67489004-67489023 None:intergenic 35.0%
AAACACTTACATGGGTCCAT+TGG - chr2.3:67488949-67488968 None:intergenic 40.0%
AAATTGAGGGACCAAAATCG+TGG - chr2.3:67490962-67490981 None:intergenic 40.0%
AGCATATCCTGTCATAGTTC+AGG - chr2.3:67490498-67490517 None:intergenic 40.0%
AGGTTGCTGTATGGATATAC+TGG + chr2.3:67490685-67490704 MS.gene05841:CDS 40.0%
AGTACCTTCGTAGAGAGATA+TGG - chr2.3:67488790-67488809 None:intergenic 40.0%
ATCCATACAGCAACCTTATG+AGG - chr2.3:67490681-67490700 None:intergenic 40.0%
ATTGAAAGGAATGGTGAACC+GGG - chr2.3:67488833-67488852 None:intergenic 40.0%
ATTGACAATTCCAGCTAGTG+TGG + chr2.3:67489104-67489123 MS.gene05841:CDS 40.0%
CATTTAGTAGGATGGAGCTT+TGG - chr2.3:67489519-67489538 None:intergenic 40.0%
CTTCGTCAGCTAAATAATCC+TGG - chr2.3:67491681-67491700 None:intergenic 40.0%
GAACTATGACAGGATATGCT+TGG + chr2.3:67490498-67490517 MS.gene05841:intron 40.0%
GAGAAAATTCAATTGGGAGG+GGG + chr2.3:67489429-67489448 MS.gene05841:intron 40.0%
GAGTCGAACATGGTTTATGA+TGG - chr2.3:67489658-67489677 None:intergenic 40.0%
GATAGAAAACTGCAGTGTTG+TGG + chr2.3:67491631-67491650 MS.gene05841:CDS 40.0%
GATCCCTGAATTTGGTCATT+CGG - chr2.3:67491302-67491321 None:intergenic 40.0%
GGAGAAAATTCAATTGGGAG+GGG + chr2.3:67489428-67489447 MS.gene05841:intron 40.0%
GGATAGAATAAGAGTGGTGA+AGG - chr2.3:67488685-67488704 None:intergenic 40.0%
GTACCTTCGTAGAGAGATAT+GGG - chr2.3:67488789-67488808 None:intergenic 40.0%
TGACAAAATGGAGTCGAACA+TGG - chr2.3:67489668-67489687 None:intergenic 40.0%
TGATCCCTGACTATTGCATA+TGG + chr2.3:67489407-67489426 MS.gene05841:intron 40.0%
TGGAACAACATCAACCTCTT+TGG + chr2.3:67491459-67491478 MS.gene05841:intron 40.0%
TTACATGGGTCCATTGGTAT+CGG - chr2.3:67488943-67488962 None:intergenic 40.0%
TTGACAATTCCAGCTAGTGT+GGG + chr2.3:67489105-67489124 MS.gene05841:CDS 40.0%
TTGGCTACTAGATCAGAATG+CGG - chr2.3:67489937-67489956 None:intergenic 40.0%
! TTTGAAGAAGTGCATTGCTG+AGG + chr2.3:67489188-67489207 MS.gene05841:CDS 40.0%
!! AGAGGTTGATGTTGTTCCAA+AGG - chr2.3:67491458-67491477 None:intergenic 40.0%
!! CTTTTGGTTGATGCCTCATA+AGG + chr2.3:67490665-67490684 MS.gene05841:CDS 40.0%
!! GTCCATTGGTATCGGTAATT+TGG - chr2.3:67488935-67488954 None:intergenic 40.0%
!! TACACACCAAAGTGTTGTTC+TGG - chr2.3:67490876-67490895 None:intergenic 40.0%
!! TTAGGTGGATTGACGCTAAA+TGG + chr2.3:67491722-67491741 MS.gene05841:CDS 40.0%
AAGGTTACTCTTTCAGCCCA+TGG - chr2.3:67489904-67489923 None:intergenic 45.0%
ACAAAGTTTCGCACACATGG+TGG - chr2.3:67489460-67489479 None:intergenic 45.0%
ACGTGGGAATGGCATATCTA+GGG - chr2.3:67491588-67491607 None:intergenic 45.0%
AGGACTGACATGCAAAGCTT+TGG - chr2.3:67489956-67489975 None:intergenic 45.0%
AGTTGAGCCACTTAAGCAAG+TGG + chr2.3:67489375-67489394 MS.gene05841:intron 45.0%
ATATGCCATTCCCACGTACA+GGG + chr2.3:67491591-67491610 MS.gene05841:CDS 45.0%
ATTATGTCCATTCGTCCTCC+AGG + chr2.3:67491522-67491541 MS.gene05841:intron 45.0%
CAAAGTTTCGCACACATGGT+GGG - chr2.3:67489459-67489478 None:intergenic 45.0%
CGCCAAATTACCGATACCAA+TGG + chr2.3:67488930-67488949 MS.gene05841:CDS 45.0%
CGCGTGAAAAGGAAGGATAA+TGG - chr2.3:67488706-67488725 None:intergenic 45.0%
GAACCCATATCTCTCTACGA+AGG + chr2.3:67488783-67488802 MS.gene05841:CDS 45.0%
GCTTCTTCCTCTTCCAATGA+CGG + chr2.3:67488756-67488775 MS.gene05841:CDS 45.0%
GGAGGACGAATGGACATAAT+GGG - chr2.3:67491522-67491541 None:intergenic 45.0%
GTAATTTGGCGAGCTTCATC+TGG - chr2.3:67488921-67488940 None:intergenic 45.0%
GTCATTGGAAGAGGAAGAAG+CGG - chr2.3:67488757-67488776 None:intergenic 45.0%
GTCTGCTCCCATATGCATTT+GGG - chr2.3:67491269-67491288 None:intergenic 45.0%
GTTACTTCCTCAGTTTCGCT+CGG + chr2.3:67490079-67490098 MS.gene05841:intron 45.0%
TACGTGGGAATGGCATATCT+AGG - chr2.3:67491589-67491608 None:intergenic 45.0%
TCAGTTCATCACCCTGAGTT+TGG + chr2.3:67490573-67490592 MS.gene05841:CDS 45.0%
TCTGGAGAAAGATGATTGGG+TGG - chr2.3:67488903-67488922 None:intergenic 45.0%
TGATGTCGAGCACAACTTGT+TGG - chr2.3:67490625-67490644 None:intergenic 45.0%
TGCCTCATAAGGTTGCTGTA+TGG + chr2.3:67490676-67490695 MS.gene05841:CDS 45.0%
TGGAGGACGAATGGACATAA+TGG - chr2.3:67491523-67491542 None:intergenic 45.0%
TGTCTGCTCCCATATGCATT+TGG - chr2.3:67491270-67491289 None:intergenic 45.0%
TGTGGATTCAGTCCACAAAG+AGG + chr2.3:67491649-67491668 MS.gene05841:CDS 45.0%
TGTTCATATCCCACACTAGC+TGG - chr2.3:67489117-67489136 None:intergenic 45.0%
TTTATAGCCTGGAGGACGAA+TGG - chr2.3:67491532-67491551 None:intergenic 45.0%
! CCTGTCATAGTTCAGGTTCA+GGG - chr2.3:67490491-67490510 None:intergenic 45.0%
! CGGATCACGCAGTTTTCTTT+TGG + chr2.3:67490649-67490668 MS.gene05841:CDS 45.0%
! TCCTGTCATAGTTCAGGTTC+AGG - chr2.3:67490492-67490511 None:intergenic 45.0%
AAAGCTTCGTCCCTGTACGT+GGG - chr2.3:67491604-67491623 None:intergenic 50.0%
AAGCAAGTCCTAGAGACAGG+TGG + chr2.3:67491698-67491717 MS.gene05841:CDS 50.0%
ACGAAGCAAGTCCTAGAGAC+AGG + chr2.3:67491695-67491714 MS.gene05841:CDS 50.0%
AGCCACTTAAGCAAGTGGTC+AGG + chr2.3:67489380-67489399 MS.gene05841:intron 50.0%
ATATGGACCGAGCGAAACTG+AGG - chr2.3:67490089-67490108 None:intergenic 50.0%
CACCTAAACCACCTGTCTCT+AGG - chr2.3:67491709-67491728 None:intergenic 50.0%
CCACTTAAGCAAGTGGTCAG+GGG + chr2.3:67489382-67489401 MS.gene05841:intron 50.0%
CCCTGAACCTGAACTATGAC+AGG + chr2.3:67490488-67490507 MS.gene05841:intron 50.0%
CTAGAGACAGGTGGTTTAGG+TGG + chr2.3:67491707-67491726 MS.gene05841:CDS 50.0%
GACAGGATATGCTTGGGAGT+TGG + chr2.3:67490505-67490524 MS.gene05841:intron 50.0%
GATATGCCATTCCCACGTAC+AGG + chr2.3:67491590-67491609 MS.gene05841:CDS 50.0%
GCCACTTAAGCAAGTGGTCA+GGG + chr2.3:67489381-67489400 MS.gene05841:intron 50.0%
GGATTGACGCTAAATGGCCA+TGG + chr2.3:67491728-67491747 MS.gene05841:CDS 50.0%
GGCACAAAGTTTCGCACACA+TGG - chr2.3:67489463-67489482 None:intergenic 50.0%
GTCCTAGAGACAGGTGGTTT+AGG + chr2.3:67491704-67491723 MS.gene05841:CDS 50.0%
GTTGTGCTCGACATCAGCAT+CGG + chr2.3:67490629-67490648 MS.gene05841:CDS 50.0%
TAAAGCTTCGTCCCTGTACG+TGG - chr2.3:67491605-67491624 None:intergenic 50.0%
TATGGGTTCAGCTCCGTCAT+TGG - chr2.3:67488772-67488791 None:intergenic 50.0%
TCCACAAAGAGGAAGCAACC+AGG + chr2.3:67491660-67491679 MS.gene05841:CDS 50.0%
TCCTGGTTGCTTCCTCTTTG+TGG - chr2.3:67491664-67491683 None:intergenic 50.0%
TTCAGCTCCGTCATTGGAAG+AGG - chr2.3:67488766-67488785 None:intergenic 50.0%
! TTCCTTTTCACGCGCTACTG+CGG + chr2.3:67488712-67488731 MS.gene05841:CDS 50.0%
CCCCTGACCACTTGCTTAAG+TGG - chr2.3:67489385-67489404 None:intergenic 55.0%
GAAAGGAATGGTGAACCGGG+TGG - chr2.3:67488830-67488849 None:intergenic 55.0%
GCAGTAGCGCGTGAAAAGGA+AGG - chr2.3:67488713-67488732 None:intergenic 55.0%
TTCGTCCCTGTACGTGGGAA+TGG - chr2.3:67491599-67491618 None:intergenic 55.0%
AGATGATTGGGTGGCGCGTG+AGG - chr2.3:67488894-67488913 None:intergenic 60.0%
AGGAATGGTGAACCGGGTGG+CGG - chr2.3:67488827-67488846 None:intergenic 60.0%
AGAGGAAGAAGCGGCGCGTG+AGG - chr2.3:67488748-67488767 None:intergenic 65.0%
CGCCGCAGTAGCGCGTGAAA+AGG - chr2.3:67488717-67488736 None:intergenic 65.0%
CTACCATCAGCGCCGCCACC+CGG + chr2.3:67488812-67488831 MS.gene05841:CDS 70.0%
TGGGTGGCGCGTGAGGTGCA+CGG - chr2.3:67488887-67488906 None:intergenic 70.0%
GCGCGTGAGGTGCACGGTCG+AGG - chr2.3:67488881-67488900 None:intergenic 75.0%
! GAACCGGGTGGCGGCGCTGA+TGG - chr2.3:67488818-67488837 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 67488673 67491756 67488673 ID=MS.gene05841
chr2.3 mRNA 67488673 67491756 67488673 ID=MS.gene05841.t1;Parent=MS.gene05841
chr2.3 exon 67488673 67488958 67488673 ID=MS.gene05841.t1.exon1;Parent=MS.gene05841.t1
chr2.3 CDS 67488673 67488958 67488673 ID=cds.MS.gene05841.t1;Parent=MS.gene05841.t1
chr2.3 exon 67489098 67489209 67489098 ID=MS.gene05841.t1.exon2;Parent=MS.gene05841.t1
chr2.3 CDS 67489098 67489209 67489098 ID=cds.MS.gene05841.t1;Parent=MS.gene05841.t1
chr2.3 exon 67489889 67489981 67489889 ID=MS.gene05841.t1.exon3;Parent=MS.gene05841.t1
chr2.3 CDS 67489889 67489981 67489889 ID=cds.MS.gene05841.t1;Parent=MS.gene05841.t1
chr2.3 exon 67490510 67490710 67490510 ID=MS.gene05841.t1.exon4;Parent=MS.gene05841.t1
chr2.3 CDS 67490510 67490710 67490510 ID=cds.MS.gene05841.t1;Parent=MS.gene05841.t1
chr2.3 exon 67491544 67491756 67491544 ID=MS.gene05841.t1.exon5;Parent=MS.gene05841.t1
chr2.3 CDS 67491544 67491756 67491544 ID=cds.MS.gene05841.t1;Parent=MS.gene05841.t1
Gene Sequence

>MS.gene05841

TCTCCATTTCCTTCACCACTCTTATTCTATCCATTATCCTTCCTTTTCACGCGCTACTGCGGCGTTTAGTAACCTCACGCGCCGCTTCTTCCTCTTCCAATGACGGAGCTGAACCCATATCTCTCTACGAAGGTACTGTCTACCATCAGCGCCGCCACCCGGTTCACCATTCCTTTCAATACAATGTTCGCTACGCACTTATTGACCTCGACCGTGCACCTCACGCGCCACCCAATCATCTTTCTCCAGATGAAGCTCGCCAAATTACCGATACCAATGGACCCATGTAAGTGTTTTTCTTCATTCTTTACTTTTTCTCAATTGGGTTCCCTCAGAACCATGCAAAATTTGATTTTTACGTTTCAATTTAAATGTAGGTGAAATTTTCTGTGTTTTTGATTCATTTGTTGAATTCAATCTTGTAGTTTGCTATTGACAATTCCAGCTAGTGTGGGATATGAACAGAATCCATTGAGTGTGTATTATTGCTATGATGTTGAAGACTCTGATACACGTTTGAAGAAGTGCATTGCTGAGGTATGTGCTAGTTAAGCTCTATTTTCTTAGTTTCTCTGACCTTTTCAATTTACACATATAATTAGTGATTCACTTGCTCAAACGAATTACTACTTTGTCGCTAAACATAGTAAGGGCATTGATTAAATTGAGAAGATTAATTAATTAGTGGTGAGGGACTTGAACAGTTGAGCCACTTAAGCAAGTGGTCAGGGGTTTGATCCCTGACTATTGCATATGGAGAAAATTCAATTGGGAGGGGGAGAACCCACCATGTGTGCGAAACTTTGTGCCTATAACATGATAACCTAACAACAACAAAAACAACCAAAGCTCCATCCTACTAAATGAGATAGCTACATGGATCAAATTACGACGCAATGTTCTATCATAAACCATATTTGGGTTCAACTCATTGATCTCTAAATCTTTCCTAACAGTTTCTCTTATAGTTTTTCTAGGTTTTCCATCATAAACCATGTTCGACTCCATTTTGTCAGTTTATTCTTCTTTATAGGATTTCTCTTGGTTGAGTGAAAAACAGTTTGTGGTGTTTTTCACTTTTATGTGGGATATTTAATTTGAATTCAGATACTTGATAATTCAGGACTTTTTTCTTTCTTCGTTTCTGTTAATATTGTCAGTAAGTAGTAAGTAGAGTATTGTGAAAACTGGTGACGATGTTTTTTTTTTGTTTCAGGTAACAAATACACCATGGGCTGAAAGAGTAACCTTTATTTTCAATCCGCATTCTGATCTAGTAGCCAAAGCTTTGCATGTCAGTCCTTTTATGGTATGTAAATTATACCATTTCTATGCAGATTTTAGGACACTTTCGTTCAAATTGCTATTTGCTCTTCAATTTTATCTATGTTTTGAGAAAAACAACAGTTACTTCCTCAGTTTCGCTCGGTCCATATATTGTGTTTTCCTCTTCTTTTGGTCATTGCATAATATGATTCAAACTGAGGAAACTGAATCAATGACAACCAACACATACATATCATGGTTGGCATTAGATGCTTAGCCTACATAGTTAACTTAAATTAATATAAAAATGACAATTAATAAAAGGGTTTGTCTAACATGTGCAATATTGCACATGTTAAGGTAACCAAGAGTAGTAACTTTTCATTGAAAACCAACCAATATTTATTCAAAAGCTACATATTTATTGTAAAAAACACAAGTTTCAAGTCAAAGTTGCTACTTTAAACTTCTTATCATGTGCAAATTTGCACATGATAGAAAAATCCTTAATAAAAATAGCTTTTCTGCATTTTTTCGGTGATCATATCTCCCTGAACCTGAACTATGACAGGATATGCTTGGGAGTTGGAATATCAAAGCAAGTGATCCTGGAGAAAATCTTAGCATATCAATTTCAGTTCATCACCCTGAGTTTGGAAACTATTTTACTGCTAGTCTGAGAGCCAACAAGTTGTGCTCGACATCAGCATCGGATCACGCAGTTTTCTTTTGGTTGATGCCTCATAAGGTTGCTGTATGGATATACTGGCATGTAAGCTTCCATTTTTAAATGATTAAGTATTTACTGCGATTTATGTTATGTTTTGTTATGATGAATTTTCATTGGCAAGCAATTTAAAATCTCACTTCGGGGTGTGACGCCATTATCTTCTTCCATGAGAAGTAAATATTTTTTGCTTGAGATTGGGAAATACCAGAACAACACTTTGGTGTGTAGACATTTGACTTAAAATGCATTTTTGGTTCCTCTATTTTGTTGTTGTTGTTGTATTTTTAAATCCACGATTTTGGTCCCTCAATTTTCAAAATAACTAACTTTTCCCTCAGTTACACATTTTGAAGCTGAATTTTGATGATGTAGCAAGTAAGATGTTTACATGACAATTCAGTCTGTGACATAATACTATTTAGGTAAATTGTTAATATCTAATTTCTAATTCACAAAAATACAATAATCTATATCACAAAAACAAACAAAAAATCATATAAAACATTGTTTATATGGATTCTCACTTAATAACTCATTTTCATGTCGCCATCTTAGTTCTCACACAATTATTTCACTTGCCAAATCATCAAAAATCAACCCAAATGCATATGGGAGCAGACATTTTGAAAACCGAATGACCAAATTCAGGGATCTAAAAATATGGGACCAAAATCGTATGAGACAAAATAGAGAACTAAAACTACATTTAAGCGTAATCAACCATTTTTGCATTTGAATTGAAAAGTGCATCCCAACTTTACTGCCTTTATTTTAATGGACATGTTGCCTTTGGAACAACATCAACCTCTTTGGACATGTTTTCAATTGATTTTGTTTGACAAGTACTGAGCCCATTATGTCCATTCGTCCTCCAGGCTATAAAATTATGGTGGAAGAATGTGAAATTTATACAACACCCTAGATATGCCATTCCCACGTACAGGGACGAAGCTTTAATACGAGATAGAAAACTGCAGTGTTGTGGATTCAGTCCACAAAGAGGAAGCAACCAGGATTATTTAGCTGACGAAGCAAGTCCTAGAGACAGGTGGTTTAGGTGGATTGACGCTAAATGGCCATGGTCGTAG
Protein sequence

>MS.gene05841.t1

SPFPSPLLFYPLSFLFTRYCGVUUPHAPLLPLPMTELNPYLSTKVLSTISAATRFTIPFNTMFATHLLTSTVHLTRHPIIFLQMKLAKLPIPMDPFCYUQFQLVWDMNRIHUVCIIAMMLKTLIHVURSALLRUQIHHGLKEUPLFSIRILIUUPKLCMSVLLWICLGVGISKQVILEKILAYQFQFITLSLETILLLVUEPTSCARHQHRITQFSFGUCLIRLLYGYTGMLUNYGGRMUNLYNTLDMPFPRTGTKLUYEIENCSVVDSVHKEEATRIIULTKQVLETGGLGGLTLNGHGR