AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70069

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70069.t1 MTR_4g016930 96.624 237 8 0 1 237 61 297 1.83e-170 471
MS.gene70069.t1 MTR_7g079520 40.909 132 74 2 107 237 49 177 3.28e-29 108
MS.gene70069.t1 MTR_4g088905 47.368 114 53 2 123 236 147 253 1.09e-26 107
MS.gene70069.t1 MTR_4g088905 35.758 165 87 7 73 231 334 485 1.46e-22 95.9
MS.gene70069.t1 MTR_4g088905 40.385 104 59 1 128 231 277 377 2.50e-20 89.4
MS.gene70069.t1 MTR_2g103130 35.417 96 59 1 136 231 55 147 1.46e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70069.t1 AT2G38270 70.513 234 63 1 2 235 65 292 2.80e-125 357
MS.gene70069.t1 AT3G54900 43.966 116 58 2 120 231 51 163 6.48e-27 102
MS.gene70069.t1 AT4G04950 41.935 124 56 2 113 236 143 250 7.01e-24 99.8
MS.gene70069.t1 AT4G04950 41.667 96 53 1 136 231 390 482 2.19e-21 92.4
MS.gene70069.t1 AT4G04950 40.000 100 57 1 132 231 279 375 6.64e-18 82.4
MS.gene70069.t1 AT3G15660 38.384 99 58 1 133 231 62 157 2.09e-20 85.1
MS.gene70069.t1 AT3G15660 38.384 99 58 1 133 231 62 157 2.09e-20 85.1

Find 70 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTACCCAAGTGGCGTTTC+TGG 0.160160 4.2:-85020036 None:intergenic
CACTTCACCTTTCTCATTCA+TGG 0.250244 4.2:-85020390 None:intergenic
TCCACATAACGGCGCACTTC+TGG 0.276069 4.2:-85020186 None:intergenic
TTGGATGAAGATTATAACTA+TGG 0.276665 4.2:+85020272 MS.gene70069:CDS
CAAGTTGGTAAAAGAGAATA+AGG 0.302408 4.2:+85020142 MS.gene70069:CDS
GCGTTTCTGGATTCATTTCC+AGG 0.311319 4.2:-85020023 None:intergenic
AAAGCTCCGGCACCGATTTC+CGG 0.329481 4.2:-85018226 None:intergenic
CGAAGATCAGCCTTCTTCTT+AGG 0.340552 4.2:-85020065 None:intergenic
GGATTCTCTCAGAGGGTAAT+AGG 0.345382 4.2:+85020206 MS.gene70069:CDS
AACTGAGAATCTTACCTTAA+CGG 0.380493 4.2:-85018255 None:intergenic
TGGGTCAAAGATTCTCTGTC+AGG 0.419390 4.2:-85019948 None:intergenic
CCATCCAAGACTTCCATGCT+TGG 0.419718 4.2:-85019968 None:intergenic
TACTATATAGGTTGGGGTAG+TGG 0.427837 4.2:+85019920 MS.gene70069:intron
AAATCGGTGCCGGAGCTTTG+TGG 0.430690 4.2:+85018230 MS.gene70069:CDS
GGAGCTTTGTGGCTCCGTTA+AGG 0.452752 4.2:+85018241 MS.gene70069:CDS
TGACCCAAGCATGGAAGTCT+TGG 0.456099 4.2:+85019964 MS.gene70069:CDS
CTCACCGTTCTTATCATAAA+CGG 0.466803 4.2:-85018153 None:intergenic
GGTAATAGGCATTCTTGAAA+AGG 0.467989 4.2:+85020220 MS.gene70069:CDS
CATCCAAGACTTCCATGCTT+GGG 0.479131 4.2:-85019967 None:intergenic
TCAGCCTTCTTCTTAGGCTG+TGG 0.482968 4.2:-85020059 None:intergenic
CACAGTCACCGGCGAGGTAT+CGG 0.486863 4.2:-85018072 None:intergenic
TGTCTCGGCTCACCGGAAAT+CGG 0.487605 4.2:+85018214 MS.gene70069:CDS
AAGATTATAACTATGGATTG+AGG 0.490227 4.2:+85020279 MS.gene70069:CDS
TTATGAGAGTGTAGATGTAT+TGG 0.491403 4.2:+85020253 MS.gene70069:CDS
GGCTCACCGGAAATCGGTGC+CGG 0.493679 4.2:+85018220 MS.gene70069:CDS
AACGGTGAGCTTCAGTTCAT+CGG 0.497572 4.2:+85018167 MS.gene70069:CDS
TCCAGAAGTGCGCCGTTATG+TGG 0.500421 4.2:+85020185 MS.gene70069:CDS
ATCATAAACGGCGTAAACTC+CGG 0.503980 4.2:-85018141 None:intergenic
AGATTATAACTATGGATTGA+GGG 0.506926 4.2:+85020280 MS.gene70069:CDS
CTGCTACTGTCTCGGCTCAC+CGG 0.511437 4.2:+85018207 MS.gene70069:CDS
TTGAATGGTGAACTTGTTGG+TGG 0.513486 4.2:+85020350 MS.gene70069:CDS
GAAGAACTTCCGTCAGGTGC+CGG 0.522260 4.2:+85018122 MS.gene70069:CDS
TAATATTGCTGCTACTGTCT+CGG 0.530231 4.2:+85018199 MS.gene70069:CDS
ACCTGACGGAAGTTCTTCTC+CGG 0.535226 4.2:-85018117 None:intergenic
ATGAATCCAGAAACGCCACT+TGG 0.543314 4.2:+85020030 MS.gene70069:CDS
TTGACCTCCATGAATGAGAA+AGG 0.544715 4.2:+85020383 MS.gene70069:CDS
ACCGGAGAAGAACTTCCGTC+AGG 0.546323 4.2:+85018116 MS.gene70069:CDS
TGAAGAGCTCGTGGACAAGT+TGG 0.551751 4.2:+85020127 MS.gene70069:CDS
TAAGGTAGTTGCATTCATCA+AGG 0.556799 4.2:+85020160 MS.gene70069:CDS
TTAACGGAGCCACAAAGCTC+CGG 0.559023 4.2:-85018239 None:intergenic
GTTAGTTGCATGTGACGACC+AGG 0.570444 4.2:-85020095 None:intergenic
CCAAGCATGGAAGTCTTGGA+TGG 0.573626 4.2:+85019968 MS.gene70069:CDS
AAGACCACAGCCTAAGAAGA+AGG 0.577244 4.2:+85020055 MS.gene70069:CDS
GAGAATCTTTGACCCAAGCA+TGG 0.586957 4.2:+85019955 MS.gene70069:CDS
CGTCCCTCTTGAAGAGCTCG+TGG 0.588084 4.2:+85020118 MS.gene70069:CDS
CACGAGCTCTTCAAGAGGGA+CGG 0.589016 4.2:-85020117 None:intergenic
AGGAACACATAAAGATCAGT+GGG 0.590962 4.2:+85019991 MS.gene70069:CDS
ATCAGTGGGAAAGTTCCACC+TGG 0.600855 4.2:+85020005 MS.gene70069:CDS
AAACCTCGCCGATACCTCGC+CGG 0.600972 4.2:+85018064 MS.gene70069:CDS
CGTTATGTGGATTCTCTCAG+AGG 0.601281 4.2:+85020198 MS.gene70069:CDS
CCTGAAAACGATGAAGTCAC+CGG 0.605156 4.2:+85018098 MS.gene70069:CDS
ATAGGCATTCTTGAAAAGGA+AGG 0.610176 4.2:+85020224 MS.gene70069:CDS
CTCTGAGAGAATCCACATAA+CGG 0.611300 4.2:-85020197 None:intergenic
TTGTCCACGAGCTCTTCAAG+AGG 0.611733 4.2:-85020122 None:intergenic
AAGGTAGTTGCATTCATCAA+GGG 0.614128 4.2:+85020161 MS.gene70069:CDS
TGAATCCAGAAACGCCACTT+GGG 0.616265 4.2:+85020031 MS.gene70069:CDS
GAGGAACACATAAAGATCAG+TGG 0.624888 4.2:+85019990 MS.gene70069:CDS
AATGAGAAAGGTGAAGTGGC+TGG 0.626698 4.2:+85020395 MS.gene70069:CDS
GCGTAAACTCCGGCACCTGA+CGG 0.641237 4.2:-85018131 None:intergenic
TTCACCTTTCTCATTCATGG+AGG 0.647990 4.2:-85020387 None:intergenic
GCTGATCTTCGATTAACACC+TGG 0.649446 4.2:+85020077 MS.gene70069:CDS
AGCATGGAAGTCTTGGATGG+AGG 0.650681 4.2:+85019971 MS.gene70069:CDS
TGTCCACGAGCTCTTCAAGA+GGG 0.653430 4.2:-85020121 None:intergenic
TACGCCGTTTATGATAAGAA+CGG 0.658415 4.2:+85018149 MS.gene70069:CDS
GTTATGTGGATTCTCTCAGA+GGG 0.661671 4.2:+85020199 MS.gene70069:CDS
CATGAATGAGAAAGGTGAAG+TGG 0.666770 4.2:+85020391 MS.gene70069:CDS
AGGCTGTGGTCTTACCCAAG+TGG 0.670747 4.2:-85020045 None:intergenic
TTTCTGGATTCATTTCCAGG+TGG 0.680654 4.2:-85020020 None:intergenic
AGGTGACACAGTCACCGGCG+AGG 0.727871 4.2:-85018078 None:intergenic
TCACCGGCGAGGTATCGGCG+AGG 0.773492 4.2:-85018067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGGTTATTTAATTGTAAAT+TGG + chr4.2:85018503-85018522 MS.gene70069:intron 15.0%
!! TGTAATATACAAACAGTATT+TGG + chr4.2:85019766-85019785 MS.gene70069:intron 20.0%
!!! AAATACCTTTGAAAAGATAA+AGG - chr4.2:85018748-85018767 None:intergenic 20.0%
!!! TAATTGTTTAGAACAATTTG+TGG + chr4.2:85018356-85018375 MS.gene70069:intron 20.0%
!!! TTTGTAAATTTTATTGCAGT+AGG + chr4.2:85018460-85018479 MS.gene70069:intron 20.0%
! AAAATCTCCAAAAGAAATTG+TGG - chr4.2:85019497-85019516 None:intergenic 25.0%
! AAAATGCAGAAAAAGACATA+TGG - chr4.2:85019722-85019741 None:intergenic 25.0%
! AAATCTCCAAAAGAAATTGT+GGG - chr4.2:85019496-85019515 None:intergenic 25.0%
! AAATGCAGAAAAAGACATAT+GGG - chr4.2:85019721-85019740 None:intergenic 25.0%
! AAATTGTCTACTATATAGGT+TGG + chr4.2:85019912-85019931 MS.gene70069:intron 25.0%
! AATTGTCTACTATATAGGTT+GGG + chr4.2:85019913-85019932 MS.gene70069:intron 25.0%
! AATTTGATCCAATTTCATTG+TGG + chr4.2:85018604-85018623 MS.gene70069:intron 25.0%
! ACAATGAAAAATTCATCATC+AGG - chr4.2:85019891-85019910 None:intergenic 25.0%
! ACTTGTGTATTTCAAAATTC+AGG - chr4.2:85018441-85018460 None:intergenic 25.0%
! ATCATCAGGAAAGTAATATT+TGG - chr4.2:85019877-85019896 None:intergenic 25.0%
! ATTTGAAAAGTCTCAAATCA+TGG - chr4.2:85019657-85019676 None:intergenic 25.0%
! ATTTGATCCAATTTCATTGT+GGG + chr4.2:85018605-85018624 MS.gene70069:intron 25.0%
! TAAAATCACATCCTTAACTT+CGG + chr4.2:85018554-85018573 MS.gene70069:intron 25.0%
! TGTGAAATTGTCTACTATAT+AGG + chr4.2:85019908-85019927 MS.gene70069:intron 25.0%
! TGTTTGTATATTACATCTGT+AGG - chr4.2:85019761-85019780 None:intergenic 25.0%
! TTGGATGAAGATTATAACTA+TGG + chr4.2:85020272-85020291 MS.gene70069:CDS 25.0%
!! AAGATTATAACTATGGATTG+AGG + chr4.2:85020279-85020298 MS.gene70069:CDS 25.0%
!! AGATTATAACTATGGATTGA+GGG + chr4.2:85020280-85020299 MS.gene70069:CDS 25.0%
!! GTGAAAAGTTTGCTTTAATT+TGG + chr4.2:85018789-85018808 MS.gene70069:intron 25.0%
AAAAATATCTGAGGGAATGT+AGG - chr4.2:85020332-85020351 None:intergenic 30.0%
AACTGAGAATCTTACCTTAA+CGG - chr4.2:85018258-85018277 None:intergenic 30.0%
ACTTCTTCAACACAATAAGT+GGG + chr4.2:85019410-85019429 MS.gene70069:intron 30.0%
AGTAACTAAGTTCGTAGAAA+TGG - chr4.2:85018674-85018693 None:intergenic 30.0%
ATTGTCTACTATATAGGTTG+GGG + chr4.2:85019914-85019933 MS.gene70069:intron 30.0%
CACCATTCAAAAATATCTGA+GGG - chr4.2:85020340-85020359 None:intergenic 30.0%
GTAACTAAGTTCGTAGAAAT+GGG - chr4.2:85018673-85018692 None:intergenic 30.0%
GTATAACAGTCTTAAAGAGT+TGG + chr4.2:85018484-85018503 MS.gene70069:intron 30.0%
TCACCATTCAAAAATATCTG+AGG - chr4.2:85020341-85020360 None:intergenic 30.0%
! ATATTTGGAGATTTAGAGCA+TGG - chr4.2:85019862-85019881 None:intergenic 30.0%
! CACTGAAAAAGTATAGCAAT+TGG + chr4.2:85020306-85020325 MS.gene70069:CDS 30.0%
! TTATGAGAGTGTAGATGTAT+TGG + chr4.2:85020253-85020272 MS.gene70069:CDS 30.0%
! TTTTTGAATGGTGAACTTGT+TGG + chr4.2:85020347-85020366 MS.gene70069:CDS 30.0%
!! CAAGTTGGTAAAAGAGAATA+AGG + chr4.2:85020142-85020161 MS.gene70069:CDS 30.0%
!! TTAGGCATGAAGGTTTTAAT+TGG + chr4.2:85018858-85018877 MS.gene70069:intron 30.0%
!! TTCCCTCAGATATTTTTGAA+TGG + chr4.2:85020335-85020354 MS.gene70069:CDS 30.0%
!!! CTTTTCAAAGGTATTTTGAG+TGG + chr4.2:85018752-85018771 MS.gene70069:intron 30.0%
!!! TAGGCATGAAGGTTTTAATT+GGG + chr4.2:85018859-85018878 MS.gene70069:intron 30.0%
AAGGTAGTTGCATTCATCAA+GGG + chr4.2:85020161-85020180 MS.gene70069:CDS 35.0%
AGGAACACATAAAGATCAGT+GGG + chr4.2:85019991-85020010 MS.gene70069:CDS 35.0%
ATAGGCATTCTTGAAAAGGA+AGG + chr4.2:85020224-85020243 MS.gene70069:CDS 35.0%
ATTCCAAATAGTCGTGACAT+TGG - chr4.2:85018819-85018838 None:intergenic 35.0%
ATTCTCAAGAGCAAGCTTAA+AGG + chr4.2:85018908-85018927 MS.gene70069:intron 35.0%
CTCACCGTTCTTATCATAAA+CGG - chr4.2:85018156-85018175 None:intergenic 35.0%
GACTGAAAAACAACCAAACA+AGG - chr4.2:85019126-85019145 None:intergenic 35.0%
GACTTCTTCAACACAATAAG+TGG + chr4.2:85019409-85019428 MS.gene70069:intron 35.0%
GGTAATAGGCATTCTTGAAA+AGG + chr4.2:85020220-85020239 MS.gene70069:CDS 35.0%
TAAGGTAGTTGCATTCATCA+AGG + chr4.2:85020160-85020179 MS.gene70069:CDS 35.0%
TAATATTGCTGCTACTGTCT+CGG + chr4.2:85018199-85018218 MS.gene70069:CDS 35.0%
TACGCCGTTTATGATAAGAA+CGG + chr4.2:85018149-85018168 MS.gene70069:CDS 35.0%
TATCCAAGAGTTACCATGTT+TGG + chr4.2:85019569-85019588 MS.gene70069:intron 35.0%
TGTGTTGAAGAAGTCTCATA+TGG - chr4.2:85019404-85019423 None:intergenic 35.0%
TTAAACTCTGTGGCTTGATT+TGG + chr4.2:85018959-85018978 MS.gene70069:intron 35.0%
TTTAGCCCAAACCATTGTTT+GGG - chr4.2:85019607-85019626 None:intergenic 35.0%
TTTCAACACTCTATTTCGCT+GGG - chr4.2:85019457-85019476 None:intergenic 35.0%
TTTCCAATGTCACGACTATT+TGG + chr4.2:85018813-85018832 MS.gene70069:intron 35.0%
! AAGAATAATTGAAGAGCACG+TGG + chr4.2:85018713-85018732 MS.gene70069:intron 35.0%
! CTGTAACCCACAATTTCTTT+TGG + chr4.2:85019487-85019506 MS.gene70069:intron 35.0%
! GAGCACCTTTATCTTTTCAA+AGG + chr4.2:85018740-85018759 MS.gene70069:intron 35.0%
! TCACGACTATTTGGAATACT+AGG + chr4.2:85018822-85018841 MS.gene70069:intron 35.0%
! TTAATTCTTGCTGCCTTGTT+TGG + chr4.2:85019110-85019129 MS.gene70069:intron 35.0%
!! TGATTGATTTTTCTGTGGAG+TGG + chr4.2:85019354-85019373 MS.gene70069:intron 35.0%
!! TGCTGTGATTGATTTTTCTG+TGG + chr4.2:85019349-85019368 MS.gene70069:intron 35.0%
!!! TGAAGGTTTTAATTGGGAGA+AGG + chr4.2:85018865-85018884 MS.gene70069:intron 35.0%
!!! TTCAAAGGTATTTTGAGTGG+AGG + chr4.2:85018755-85018774 MS.gene70069:intron 35.0%
ACATCCTTAACTTCGGAGTT+TGG + chr4.2:85018561-85018580 MS.gene70069:intron 40.0%
AGTTGCTCCCACAATGAAAT+TGG - chr4.2:85018615-85018634 None:intergenic 40.0%
ATCATAAACGGCGTAAACTC+CGG - chr4.2:85018144-85018163 None:intergenic 40.0%
ATGACCAAACTCCGAAGTTA+AGG - chr4.2:85018568-85018587 None:intergenic 40.0%
CACTTCACCTTTCTCATTCA+TGG - chr4.2:85020393-85020412 None:intergenic 40.0%
CATGAATGAGAAAGGTGAAG+TGG + chr4.2:85020391-85020410 MS.gene70069:CDS 40.0%
CTCTTGGATAGATTCAAGTG+TGG - chr4.2:85019559-85019578 None:intergenic 40.0%
CTTTAGCCCAAACCATTGTT+TGG - chr4.2:85019608-85019627 None:intergenic 40.0%
CTTTCAACACTCTATTTCGC+TGG - chr4.2:85019458-85019477 None:intergenic 40.0%
GAACCAAACATGGTAACTCT+TGG - chr4.2:85019575-85019594 None:intergenic 40.0%
GAGGAACACATAAAGATCAG+TGG + chr4.2:85019990-85020009 MS.gene70069:CDS 40.0%
GCATTAATGTAGCTTGAGGA+TGG + chr4.2:85018378-85018397 MS.gene70069:intron 40.0%
GTCGAATGTTTCTTGTGTAG+CGG + chr4.2:85019285-85019304 MS.gene70069:intron 40.0%
GTTATGTGGATTCTCTCAGA+GGG + chr4.2:85020199-85020218 MS.gene70069:CDS 40.0%
TCAGCACAAAACTCTCAATC+AGG - chr4.2:85019326-85019345 None:intergenic 40.0%
TGTGGCATTAATGTAGCTTG+AGG + chr4.2:85018374-85018393 MS.gene70069:intron 40.0%
TTCACCTTTCTCATTCATGG+AGG - chr4.2:85020390-85020409 None:intergenic 40.0%
TTGACCTCCATGAATGAGAA+AGG + chr4.2:85020383-85020402 MS.gene70069:CDS 40.0%
TTGGAATACTAGGAATGCCA+CGG + chr4.2:85018832-85018851 MS.gene70069:intron 40.0%
TTTCTGGATTCATTTCCAGG+TGG - chr4.2:85020023-85020042 None:intergenic 40.0%
TTTGGCCCAAACAATGGTTT+GGG + chr4.2:85019599-85019618 MS.gene70069:intron 40.0%
! CAAACTTTTCACGAACCTTC+CGG - chr4.2:85018781-85018800 None:intergenic 40.0%
! CTCTGAGAGAATCCACATAA+CGG - chr4.2:85020200-85020219 None:intergenic 40.0%
! GTGCAATTCTGTTTTGCAGT+CGG + chr4.2:85019201-85019220 MS.gene70069:intron 40.0%
! TACTATATAGGTTGGGGTAG+TGG + chr4.2:85019920-85019939 MS.gene70069:intron 40.0%
! TAGCTTTTCGCTTAACCAGT+GGG + chr4.2:85018934-85018953 MS.gene70069:intron 40.0%
! TTGTTTGCTTGAGGGTGTAT+GGG + chr4.2:85019049-85019068 MS.gene70069:intron 40.0%
!! ACCATGTTTGGTTCTCTGTT+TGG + chr4.2:85019581-85019600 MS.gene70069:intron 40.0%
!! TGTGGAGTGGCTTTTTAGTA+TGG + chr4.2:85019367-85019386 MS.gene70069:intron 40.0%
!! TTAGCTTTTCGCTTAACCAG+TGG + chr4.2:85018933-85018952 MS.gene70069:intron 40.0%
!! TTGAATGGTGAACTTGTTGG+TGG + chr4.2:85020350-85020369 MS.gene70069:CDS 40.0%
!!! AGAAGGCGGAAATTTTAGCT+TGG + chr4.2:85018882-85018901 MS.gene70069:intron 40.0%
!!! AGGTTTTAATTGGGAGAAGG+CGG + chr4.2:85018868-85018887 MS.gene70069:intron 40.0%
AACGGTGAGCTTCAGTTCAT+CGG + chr4.2:85018167-85018186 MS.gene70069:CDS 45.0%
AAGACCACAGCCTAAGAAGA+AGG + chr4.2:85020055-85020074 MS.gene70069:CDS 45.0%
AAGCAATACCAGAACAGGCA+AGG - chr4.2:85019004-85019023 None:intergenic 45.0%
AATGAGAAAGGTGAAGTGGC+TGG + chr4.2:85020395-85020414 MS.gene70069:CDS 45.0%
ACACAAGAAACATTCGACCC+CGG - chr4.2:85019283-85019302 None:intergenic 45.0%
ACTTCGGAGTTTGGTCATGA+CGG + chr4.2:85018570-85018589 MS.gene70069:intron 45.0%
AGCAATACCAGAACAGGCAA+GGG - chr4.2:85019003-85019022 None:intergenic 45.0%
ATGAATCCAGAAACGCCACT+TGG + chr4.2:85020030-85020049 MS.gene70069:CDS 45.0%
CATCCAAGACTTCCATGCTT+GGG - chr4.2:85019970-85019989 None:intergenic 45.0%
CATCTTCATCTGTGCATTGG+AGG + chr4.2:85019171-85019190 MS.gene70069:intron 45.0%
CCTGAAAACGATGAAGTCAC+CGG + chr4.2:85018098-85018117 MS.gene70069:CDS 45.0%
CGAAGATCAGCCTTCTTCTT+AGG - chr4.2:85020068-85020087 None:intergenic 45.0%
CGTTATGTGGATTCTCTCAG+AGG + chr4.2:85020198-85020217 MS.gene70069:CDS 45.0%
CTCAATCAGGTAAAGATCCC+TGG - chr4.2:85019313-85019332 None:intergenic 45.0%
CTGCAAGCAAAGCTGCAAAA+AGG - chr4.2:85019148-85019167 None:intergenic 45.0%
CTGCATCTTCATCTGTGCAT+TGG + chr4.2:85019168-85019187 MS.gene70069:intron 45.0%
GAGAATCTTTGACCCAAGCA+TGG + chr4.2:85019955-85019974 MS.gene70069:CDS 45.0%
GCCAAACAGAGAACCAAACA+TGG - chr4.2:85019585-85019604 None:intergenic 45.0%
GCTGATCTTCGATTAACACC+TGG + chr4.2:85020077-85020096 MS.gene70069:CDS 45.0%
GGATTCTCTCAGAGGGTAAT+AGG + chr4.2:85020206-85020225 MS.gene70069:CDS 45.0%
GTGTGGACAAACATAGAGCA+TGG - chr4.2:85019542-85019561 None:intergenic 45.0%
GTTTGGCCCAAACAATGGTT+TGG + chr4.2:85019598-85019617 MS.gene70069:intron 45.0%
TCTCTGTTTGGCCCAAACAA+TGG + chr4.2:85019593-85019612 MS.gene70069:intron 45.0%
TGAATCCAGAAACGCCACTT+GGG + chr4.2:85020031-85020050 MS.gene70069:CDS 45.0%
TGACCAAGCAATACCAGAAC+AGG - chr4.2:85019009-85019028 None:intergenic 45.0%
TGGGTCAAAGATTCTCTGTC+AGG - chr4.2:85019951-85019970 None:intergenic 45.0%
TGTTCTGGTATTGCTTGGTC+AGG + chr4.2:85019008-85019027 MS.gene70069:intron 45.0%
TTGCCTGTTCTGGTATTGCT+TGG + chr4.2:85019003-85019022 MS.gene70069:intron 45.0%
TTTCTTGTGTAGCGGTTCCA+GGG + chr4.2:85019293-85019312 MS.gene70069:intron 45.0%
! CCACAGAGTTTAAAACCCAC+TGG - chr4.2:85018952-85018971 None:intergenic 45.0%
! CTTGTTTGCTTGAGGGTGTA+TGG + chr4.2:85019048-85019067 MS.gene70069:intron 45.0%
! GCGTTTCTGGATTCATTTCC+AGG - chr4.2:85020026-85020045 None:intergenic 45.0%
! GTCGCAGCAGTTTCATTTTC+CGG + chr4.2:85019261-85019280 MS.gene70069:intron 45.0%
! TAATTGAAGAGCACGTGGAC+AGG + chr4.2:85018718-85018737 MS.gene70069:intron 45.0%
! TCGCAGCAGTTTCATTTTCC+GGG + chr4.2:85019262-85019281 MS.gene70069:intron 45.0%
!! AAGGTATTTTGAGTGGAGGC+CGG + chr4.2:85018759-85018778 MS.gene70069:intron 45.0%
!!! AGGCGGAAATTTTAGCTTGG+AGG + chr4.2:85018885-85018904 MS.gene70069:intron 45.0%
!!! CCAGTGGGTTTTAAACTCTG+TGG + chr4.2:85018949-85018968 MS.gene70069:intron 45.0%
!!! TTTTCAGGTGACACAGTCAC+CGG - chr4.2:85018086-85018105 None:intergenic 45.0%
!!! AAAAAAAACAATATTTTTAA+CGG - chr4.2:85019804-85019823 None:intergenic 5.0%
ACACCCTCAAGCAAACAAGC+AGG - chr4.2:85019047-85019066 None:intergenic 50.0%
ACCTGACGGAAGTTCTTCTC+CGG - chr4.2:85018120-85018139 None:intergenic 50.0%
ACCTTCATGCCTAAAAGCCG+TGG - chr4.2:85018852-85018871 None:intergenic 50.0%
AGATAGTGCGAGGTTAGCAG+GGG + chr4.2:85019081-85019100 MS.gene70069:intron 50.0%
AGCATGGAAGTCTTGGATGG+AGG + chr4.2:85019971-85019990 MS.gene70069:CDS 50.0%
AGTTAAGCCCTTGCCTGTTC+TGG + chr4.2:85018993-85019012 MS.gene70069:intron 50.0%
ATCAGTGGGAAAGTTCCACC+TGG + chr4.2:85020005-85020024 MS.gene70069:CDS 50.0%
CACCCTCAAGCAAACAAGCA+GGG - chr4.2:85019046-85019065 None:intergenic 50.0%
CCAAGCATGGAAGTCTTGGA+TGG + chr4.2:85019968-85019987 MS.gene70069:CDS 50.0%
CCATCCAAGACTTCCATGCT+TGG - chr4.2:85019971-85019990 None:intergenic 50.0%
GAGATAGTGCGAGGTTAGCA+GGG + chr4.2:85019080-85019099 MS.gene70069:intron 50.0%
GTCCCTGCTTGTTTGCTTGA+GGG + chr4.2:85019041-85019060 MS.gene70069:intron 50.0%
GTTAGTTGCATGTGACGACC+AGG - chr4.2:85020098-85020117 None:intergenic 50.0%
GTTTCTTGTGTAGCGGTTCC+AGG + chr4.2:85019292-85019311 MS.gene70069:intron 50.0%
TCAGCCTTCTTCTTAGGCTG+TGG - chr4.2:85020062-85020081 None:intergenic 50.0%
TCTTACCCAAGTGGCGTTTC+TGG - chr4.2:85020039-85020058 None:intergenic 50.0%
TGAAGAGCTCGTGGACAAGT+TGG + chr4.2:85020127-85020146 MS.gene70069:CDS 50.0%
TGACCCAAGCATGGAAGTCT+TGG + chr4.2:85019964-85019983 MS.gene70069:CDS 50.0%
TGTCCACGAGCTCTTCAAGA+GGG - chr4.2:85020124-85020143 None:intergenic 50.0%
TGTCCCTGCTTGTTTGCTTG+AGG + chr4.2:85019040-85019059 MS.gene70069:intron 50.0%
TTAACGGAGCCACAAAGCTC+CGG - chr4.2:85018242-85018261 None:intergenic 50.0%
TTGTCCACGAGCTCTTCAAG+AGG - chr4.2:85020125-85020144 None:intergenic 50.0%
! CCGGTGACTTCATCGTTTTC+AGG - chr4.2:85018101-85018120 None:intergenic 50.0%
! CGCAGCAGTTTCATTTTCCG+GGG + chr4.2:85019263-85019282 MS.gene70069:intron 50.0%
! CTAGGAATGCCACGGCTTTT+AGG + chr4.2:85018840-85018859 MS.gene70069:intron 50.0%
!! GGCTTGATTTGGAGCAAGCA+GGG + chr4.2:85018970-85018989 MS.gene70069:intron 50.0%
!! TATTTTGAGTGGAGGCCGGA+AGG + chr4.2:85018763-85018782 MS.gene70069:intron 50.0%
!! TGGCTTGATTTGGAGCAAGC+AGG + chr4.2:85018969-85018988 MS.gene70069:intron 50.0%
ACCGGAGAAGAACTTCCGTC+AGG + chr4.2:85018116-85018135 MS.gene70069:CDS 55.0%
CACGAGCTCTTCAAGAGGGA+CGG - chr4.2:85020120-85020139 None:intergenic 55.0%
CGAGATAGTGCGAGGTTAGC+AGG + chr4.2:85019079-85019098 MS.gene70069:intron 55.0%
GAAGAACTTCCGTCAGGTGC+CGG + chr4.2:85018122-85018141 MS.gene70069:CDS 55.0%
GGTATTGCTTGGTCAGGTGC+TGG + chr4.2:85019014-85019033 MS.gene70069:intron 55.0%
TCCACATAACGGCGCACTTC+TGG - chr4.2:85020189-85020208 None:intergenic 55.0%
TGTCTCGGCTCACCGGAAAT+CGG + chr4.2:85018214-85018233 MS.gene70069:CDS 55.0%
! AGGCTGTGGTCTTACCCAAG+TGG - chr4.2:85020048-85020067 None:intergenic 55.0%
! CTGTTTTGCAGTCGGTGTGC+TGG + chr4.2:85019209-85019228 MS.gene70069:intron 55.0%
! CTTGAGGGTGTATGGGTCGA+AGG + chr4.2:85019056-85019075 MS.gene70069:intron 55.0%
! GCCACGGCTTTTAGGCATGA+AGG + chr4.2:85018848-85018867 MS.gene70069:intron 55.0%
! GGAGCTTTGTGGCTCCGTTA+AGG + chr4.2:85018241-85018260 MS.gene70069:CDS 55.0%
! TCCAGAAGTGCGCCGTTATG+TGG + chr4.2:85020185-85020204 MS.gene70069:CDS 55.0%
!! AAAGCTCCGGCACCGATTTC+CGG - chr4.2:85018229-85018248 None:intergenic 55.0%
!! AAATCGGTGCCGGAGCTTTG+TGG + chr4.2:85018230-85018249 MS.gene70069:CDS 55.0%
AAACCTCGCCGATACCTCGC+CGG + chr4.2:85018064-85018083 MS.gene70069:CDS 60.0%
CACAGTCACCGGCGAGGTAT+CGG - chr4.2:85018075-85018094 None:intergenic 60.0%
CGTCCCTCTTGAAGAGCTCG+TGG + chr4.2:85020118-85020137 MS.gene70069:CDS 60.0%
CTGCTACTGTCTCGGCTCAC+CGG + chr4.2:85018207-85018226 MS.gene70069:CDS 60.0%
GCGTAAACTCCGGCACCTGA+CGG - chr4.2:85018134-85018153 None:intergenic 60.0%
GTCGAAGGCGAGATAGTGCG+AGG + chr4.2:85019071-85019090 MS.gene70069:intron 60.0%
AGGTGACACAGTCACCGGCG+AGG - chr4.2:85018081-85018100 None:intergenic 65.0%
GGCTCACCGGAAATCGGTGC+CGG + chr4.2:85018220-85018239 MS.gene70069:CDS 65.0%
GTGCGAGGTTAGCAGGGGCT+TGG + chr4.2:85019086-85019105 MS.gene70069:intron 65.0%
!! TCACCGGCGAGGTATCGGCG+AGG - chr4.2:85018070-85018089 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 85018053 85020433 85018053 ID=MS.gene70069
chr4.2 mRNA 85018053 85020433 85018053 ID=MS.gene70069.t1;Parent=MS.gene70069
chr4.2 exon 85018053 85018262 85018053 ID=MS.gene70069.t1.exon1;Parent=MS.gene70069.t1
chr4.2 CDS 85018053 85018262 85018053 ID=cds.MS.gene70069.t1;Parent=MS.gene70069.t1
chr4.2 exon 85019930 85020433 85019930 ID=MS.gene70069.t1.exon2;Parent=MS.gene70069.t1
chr4.2 CDS 85019930 85020433 85019930 ID=cds.MS.gene70069.t1;Parent=MS.gene70069.t1
Gene Sequence

>MS.gene70069

ATGGCAGTGAAAAACCTCGCCGATACCTCGCCGGTGACTGTGTCACCTGAAAACGATGAAGTCACCGGAGAAGAACTTCCGTCAGGTGCCGGAGTTTACGCCGTTTATGATAAGAACGGTGAGCTTCAGTTCATCGGATTGTCGCGTAATATTGCTGCTACTGTCTCGGCTCACCGGAAATCGGTGCCGGAGCTTTGTGGCTCCGTTAAGGTAAGATTCTCAGTTTTGAATTGAAGTGTTATCGATAAATTTTCAGTTATTTATTCTCATTTTTTTTTTATCTTTTTTCTAAATTAAATGAAATAATTGTTTAGAACAATTTGTGGCATTAATGTAGCTTGAGGATGGATGCACTTACATTATTGAATAACTATTTACATTGAAACCTGAATTTTGAAATACACAAGTTTGTAAATTTTATTGCAGTAGGTGTATAACAGTCTTAAAGAGTTGGTTATTTAATTGTAAATTGGAAGAGAAAAAATTAAAAAAATTGCATCATAAAATCACATCCTTAACTTCGGAGTTTGGTCATGACGGAGATTGTTTTGAATTTGATCCAATTTCATTGTGGGAGCAACTTAATGTTGTATTGTTGAGTTTCAATGACTTCACTTCCCATTTCTACGAACTTAGTTACTATTAGTTAGTGTCTTCTCAAAGAATAATTGAAGAGCACGTGGACAGGAGCACCTTTATCTTTTCAAAGGTATTTTGAGTGGAGGCCGGAAGGTTCGTGAAAAGTTTGCTTTAATTTGGTTTTCCAATGTCACGACTATTTGGAATACTAGGAATGCCACGGCTTTTAGGCATGAAGGTTTTAATTGGGAGAAGGCGGAAATTTTAGCTTGGAGGATTCTCAAGAGCAAGCTTAAAGGTTTTAGCTTTTCGCTTAACCAGTGGGTTTTAAACTCTGTGGCTTGATTTGGAGCAAGCAGGGAGTTAAGCCCTTGCCTGTTCTGGTATTGCTTGGTCAGGTGCTGGTTGTGTCCCTGCTTGTTTGCTTGAGGGTGTATGGGTCGAAGGCGAGATAGTGCGAGGTTAGCAGGGGCTTGGCTTAATTCTTGCTGCCTTGTTTGGTTGTTTTTCAGTCCTTTTTGCAGCTTTGCTTGCAGCTGCATCTTCATCTGTGCATTGGAGGTTTAGCAGTGCAATTCTGTTTTGCAGTCGGTGTGCTGGAAGCAATTTTTCATTGTGTTTCGAATTCTGTCGCAGCAGTTTCATTTTCCGGGGTCGAATGTTTCTTGTGTAGCGGTTCCAGGGATCTTTACCTGATTGAGAGTTTTGTGCTGATACTGCTGTGATTGATTTTTCTGTGGAGTGGCTTTTTAGTATGGTTTGTCAAATGCCATATGAGACTTCTTCAACACAATAAGTGGGCACAATTTAGTGACGTCAGAACCCAGCGAAATAGAGTGTTGAAAGAGTGTGCTTCTGTAACCCACAATTTCTTTTGGAGATTTTCTCTTTTAGTTAGAAAACAGTACCATGCTCTATGTTTGTCCACACTTGAATCTATCCAAGAGTTACCATGTTTGGTTCTCTGTTTGGCCCAAACAATGGTTTGGGCTAAAGCTAAAAGCAACAAGCTTTTATATAGACCATGATTTGAGACTTTTCAAATGTGAAATTGAACATGCACTCAGTATAACTCACACGTCATGACCCATATGTCTTTTTCTGCATTTTAAAATCATTGAAAACACCTACAGATGTAATATACAAACAGTATTTGGAAGTGATATGATCCGTTAAAAATATTGTTTTTTTTTTATTCTTGCACTGTTTTTATTCTGATCAAGATCACCATGCTCTAAATCTCCAAATATTACTTTCCTGATGATGAATTTTTCATTGTGAAATTGTCTACTATATAGGTTGGGGTAGTGGATGAACCTGACAGAGAATCTTTGACCCAAGCATGGAAGTCTTGGATGGAGGAACACATAAAGATCAGTGGGAAAGTTCCACCTGGAAATGAATCCAGAAACGCCACTTGGGTAAGACCACAGCCTAAGAAGAAGGCTGATCTTCGATTAACACCTGGTCGTCACATGCAACTAACCGTCCCTCTTGAAGAGCTCGTGGACAAGTTGGTAAAAGAGAATAAGGTAGTTGCATTCATCAAGGGATCCAGAAGTGCGCCGTTATGTGGATTCTCTCAGAGGGTAATAGGCATTCTTGAAAAGGAAGGAGTAGATTATGAGAGTGTAGATGTATTGGATGAAGATTATAACTATGGATTGAGGGAGACACTGAAAAAGTATAGCAATTGGCCTACATTCCCTCAGATATTTTTGAATGGTGAACTTGTTGGTGGTTGTGATATATTGACCTCCATGAATGAGAAAGGTGAAGTGGCTGGTTTGTTCAAAAAGTAA
Protein sequence

>MS.gene70069.t1

MAVKNLADTSPVTVSPENDEVTGEELPSGAGVYAVYDKNGELQFIGLSRNIAATVSAHRKSVPELCGSVKVGVVDEPDRESLTQAWKSWMEEHIKISGKVPPGNESRNATWVRPQPKKKADLRLTPGRHMQLTVPLEELVDKLVKENKVVAFIKGSRSAPLCGFSQRVIGILEKEGVDYESVDVLDEDYNYGLRETLKKYSNWPTFPQIFLNGELVGGCDILTSMNEKGEVAGLFKK