AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074598


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074598.t1 MTR_4g088920 89.474 152 16 0 7 158 1 152 1.76e-92 287
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074598.t1 AT1G65070 47.581 124 61 1 34 153 40 163 7.43e-29 111
MS.gene074598.t1 AT1G65070 47.581 124 61 1 34 153 40 163 7.56e-29 111
MS.gene074598.t1 AT1G65070 47.581 124 61 1 34 153 40 163 7.60e-29 111
MS.gene074598.t1 AT1G65070 47.581 124 61 1 34 153 40 163 8.65e-29 111

Find 46 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACACTCGCTGCTTCC+AGG 0.290821 4.3:+24160099 MS.gene074598:CDS
ATTCTCTCCGTTGCTGTTTC+CGG 0.310263 4.3:+24160041 MS.gene074598:CDS
AGGTTTGATCGAGAAGCTTT+TGG 0.334460 4.3:-24159956 None:intergenic
AGGGATTGAGGGTTTGTTGA+TGG 0.339868 4.3:-24159751 None:intergenic
GGGATTGAGGGTTTGTTGAT+GGG 0.370731 4.3:-24159750 None:intergenic
AGCATTCACTTCCACTTCAA+TGG 0.372505 4.3:+24159878 MS.gene074598:CDS
AAGGAGAAGCGAGGGATTGA+GGG 0.374390 4.3:-24159762 None:intergenic
ACGGGCGTTGTTGGCGGCGG+AGG 0.376941 4.3:-24159904 None:intergenic
GAGTACGTCCGACGGGTAAA+CGG 0.383430 4.3:-24159923 None:intergenic
GAAGGAGAAGCGAGGGATTG+AGG 0.404110 4.3:-24159763 None:intergenic
CATTCACACAGGTTTGAACT+TGG 0.408717 4.3:+24160164 MS.gene074598:CDS
GACGGGTAAACGGGCGTTGT+TGG 0.416611 4.3:-24159913 None:intergenic
GTAGCAGAAGCAAGGTTTGA+AGG 0.434423 4.3:-24159781 None:intergenic
CTTCGAACGGTGCAAAGCTC+GGG 0.437748 4.3:-24160080 None:intergenic
TGCTGAGAGTTGCTTGCATA+TGG 0.449310 4.3:-24159859 None:intergenic
GCATTCACTTCCACTTCAAT+GGG 0.457389 4.3:+24159879 MS.gene074598:CDS
GGATTGAGGGTTTGTTGATG+GGG 0.474954 4.3:-24159749 None:intergenic
AGTACGTCCGACGGGTAAAC+GGG 0.478043 4.3:-24159922 None:intergenic
GGGTAAACGGGCGTTGTTGG+CGG 0.478868 4.3:-24159910 None:intergenic
TAAACGGGCGTTGTTGGCGG+CGG 0.480719 4.3:-24159907 None:intergenic
TCTTCGAACGGTGCAAAGCT+CGG 0.496479 4.3:-24160081 None:intergenic
GATGGGGATGAAGAGGTTGC+AGG 0.501851 4.3:-24159733 None:intergenic
GTTTGATAGTTTGAAACACT+TGG 0.502558 4.3:+24160127 MS.gene074598:CDS
CAAACTATCAAACAATTCCC+TGG 0.528874 4.3:-24160117 None:intergenic
GTTTGTTGATGGGGATGAAG+AGG 0.545453 4.3:-24159740 None:intergenic
GGATTGGACTGAGTTGGACT+CGG 0.547239 4.3:-24159808 None:intergenic
AAGGTTTGAAGGAGAAGCGA+GGG 0.550811 4.3:-24159770 None:intergenic
TTGTTGTTGAGGAATGAGTC+TGG 0.550825 4.3:-24159985 None:intergenic
GTCTGTTGGAGTACGTCCGA+CGG 0.557543 4.3:-24159931 None:intergenic
AAGAACACTCGCTGCTTCCA+GGG 0.558472 4.3:+24160100 MS.gene074598:CDS
AGGTTGCAGGTGAGTTGCAT+CGG 0.562147 4.3:-24159720 None:intergenic
TCTGTTGGAGTACGTCCGAC+GGG 0.568179 4.3:-24159930 None:intergenic
ATAGCCTCAAAACTCTCGAA+TGG 0.578502 4.3:+24159832 MS.gene074598:CDS
AGGAATGAGTCTGGCAGCGG+AGG 0.579503 4.3:-24159976 None:intergenic
GCTATCGGATTGGACTGAGT+TGG 0.590319 4.3:-24159814 None:intergenic
TTGAGGAATGAGTCTGGCAG+CGG 0.591996 4.3:-24159979 None:intergenic
GGCGGAGGAGCCCATTGAAG+TGG 0.595273 4.3:-24159889 None:intergenic
CTGTATCTAATCATTCACAC+AGG 0.610846 4.3:+24160153 MS.gene074598:CDS
GGGAATAGTCAACAATCTAC+CGG 0.610891 4.3:-24160060 None:intergenic
TCGGTTGAGTAGCAGAAGCA+AGG 0.613416 4.3:-24159789 None:intergenic
AACAACGCCCGTTTACCCGT+CGG 0.621448 4.3:+24159915 MS.gene074598:CDS
CAAGGTTTGAAGGAGAAGCG+AGG 0.631968 4.3:-24159771 None:intergenic
GAAAAGTCGAGTTGTTGTTG+AGG 0.632731 4.3:-24159996 None:intergenic
CAATCTACCGGAAACAGCAA+CGG 0.634527 4.3:-24160048 None:intergenic
AGCAGCGAGTGTTCTTCGAA+CGG 0.642225 4.3:-24160093 None:intergenic
GCAGGTGAGTTGCATCGGTG+TGG 0.676745 4.3:-24159715 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTTGATAGTTTGAAACACT+TGG + chr4.3:24160127-24160146 MS.gene074598:CDS 30.0%
CAAACTATCAAACAATTCCC+TGG - chr4.3:24160120-24160139 None:intergenic 35.0%
CTGTATCTAATCATTCACAC+AGG + chr4.3:24160153-24160172 MS.gene074598:CDS 35.0%
AGCATTCACTTCCACTTCAA+TGG + chr4.3:24159878-24159897 MS.gene074598:CDS 40.0%
ATAGCCTCAAAACTCTCGAA+TGG + chr4.3:24159832-24159851 MS.gene074598:CDS 40.0%
CATTCACACAGGTTTGAACT+TGG + chr4.3:24160164-24160183 MS.gene074598:CDS 40.0%
GCATTCACTTCCACTTCAAT+GGG + chr4.3:24159879-24159898 MS.gene074598:CDS 40.0%
GGGAATAGTCAACAATCTAC+CGG - chr4.3:24160063-24160082 None:intergenic 40.0%
! AGGTTTGATCGAGAAGCTTT+TGG - chr4.3:24159959-24159978 None:intergenic 40.0%
! AGTTCCATTCGAGAGTTTTG+AGG - chr4.3:24159839-24159858 None:intergenic 40.0%
! CAACAACAACTCGACTTTTC+TGG + chr4.3:24159999-24160018 MS.gene074598:CDS 40.0%
! GAAAAGTCGAGTTGTTGTTG+AGG - chr4.3:24159999-24160018 None:intergenic 40.0%
! TTGTTGTTGAGGAATGAGTC+TGG - chr4.3:24159988-24160007 None:intergenic 40.0%
!!! TTCGAGAGTTTTGAGGCTAT+CGG - chr4.3:24159832-24159851 None:intergenic 40.0%
AAGGTTTGAAGGAGAAGCGA+GGG - chr4.3:24159773-24159792 None:intergenic 45.0%
CAATCTACCGGAAACAGCAA+CGG - chr4.3:24160051-24160070 None:intergenic 45.0%
GTAGCAGAAGCAAGGTTTGA+AGG - chr4.3:24159784-24159803 None:intergenic 45.0%
TGCTGAGAGTTGCTTGCATA+TGG - chr4.3:24159862-24159881 None:intergenic 45.0%
! GTTTGTTGATGGGGATGAAG+AGG - chr4.3:24159743-24159762 None:intergenic 45.0%
!! AGAAGCTTTTGGCTGTCTGT+TGG - chr4.3:24159948-24159967 None:intergenic 45.0%
!! AGGGATTGAGGGTTTGTTGA+TGG - chr4.3:24159754-24159773 None:intergenic 45.0%
!! ATTCTCTCCGTTGCTGTTTC+CGG + chr4.3:24160041-24160060 MS.gene074598:CDS 45.0%
!! GGATTGAGGGTTTGTTGATG+GGG - chr4.3:24159752-24159771 None:intergenic 45.0%
!! GGGATTGAGGGTTTGTTGAT+GGG - chr4.3:24159753-24159772 None:intergenic 45.0%
!!! GAGTTTTGAGGCTATCGGAT+TGG - chr4.3:24159827-24159846 None:intergenic 45.0%
AAGAACACTCGCTGCTTCCA+GGG + chr4.3:24160100-24160119 MS.gene074598:CDS 50.0%
AAGGAGAAGCGAGGGATTGA+GGG - chr4.3:24159765-24159784 None:intergenic 50.0%
AGCAGCGAGTGTTCTTCGAA+CGG - chr4.3:24160096-24160115 None:intergenic 50.0%
AGGTTGCAGGTGAGTTGCAT+CGG - chr4.3:24159723-24159742 None:intergenic 50.0%
CAAGGTTTGAAGGAGAAGCG+AGG - chr4.3:24159774-24159793 None:intergenic 50.0%
TCGGTTGAGTAGCAGAAGCA+AGG - chr4.3:24159792-24159811 None:intergenic 50.0%
TCTTCGAACGGTGCAAAGCT+CGG - chr4.3:24160084-24160103 None:intergenic 50.0%
TTGAGGAATGAGTCTGGCAG+CGG - chr4.3:24159982-24160001 None:intergenic 50.0%
! GCTATCGGATTGGACTGAGT+TGG - chr4.3:24159817-24159836 None:intergenic 50.0%
! GGATTGGACTGAGTTGGACT+CGG - chr4.3:24159811-24159830 None:intergenic 50.0%
AACAACGCCCGTTTACCCGT+CGG + chr4.3:24159915-24159934 MS.gene074598:CDS 55.0%
AGTACGTCCGACGGGTAAAC+GGG - chr4.3:24159925-24159944 None:intergenic 55.0%
CTTCGAACGGTGCAAAGCTC+GGG - chr4.3:24160083-24160102 None:intergenic 55.0%
GAAGAACACTCGCTGCTTCC+AGG + chr4.3:24160099-24160118 MS.gene074598:CDS 55.0%
GAAGGAGAAGCGAGGGATTG+AGG - chr4.3:24159766-24159785 None:intergenic 55.0%
GAGTACGTCCGACGGGTAAA+CGG - chr4.3:24159926-24159945 None:intergenic 55.0%
GATGGGGATGAAGAGGTTGC+AGG - chr4.3:24159736-24159755 None:intergenic 55.0%
GTCTGTTGGAGTACGTCCGA+CGG - chr4.3:24159934-24159953 None:intergenic 55.0%
TCTGTTGGAGTACGTCCGAC+GGG - chr4.3:24159933-24159952 None:intergenic 55.0%
AGGAATGAGTCTGGCAGCGG+AGG - chr4.3:24159979-24159998 None:intergenic 60.0%
! GACGGGTAAACGGGCGTTGT+TGG - chr4.3:24159916-24159935 None:intergenic 60.0%
! GCAGGTGAGTTGCATCGGTG+TGG - chr4.3:24159718-24159737 None:intergenic 60.0%
! GGGTAAACGGGCGTTGTTGG+CGG - chr4.3:24159913-24159932 None:intergenic 60.0%
! TAAACGGGCGTTGTTGGCGG+CGG - chr4.3:24159910-24159929 None:intergenic 60.0%
GGCGGAGGAGCCCATTGAAG+TGG - chr4.3:24159892-24159911 None:intergenic 65.0%
! ACGGGCGTTGTTGGCGGCGG+AGG - chr4.3:24159907-24159926 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 24159705 24160193 24159705 ID=MS.gene074598
chr4.3 mRNA 24159705 24160193 24159705 ID=MS.gene074598.t1;Parent=MS.gene074598
chr4.3 exon 24159705 24160193 24159705 ID=MS.gene074598.t1.exon1;Parent=MS.gene074598.t1
chr4.3 CDS 24159705 24160193 24159705 ID=cds.MS.gene074598.t1;Parent=MS.gene074598.t1
Gene Sequence

>MS.gene074598

CAGCGAGTCACCACACCGATGCAACTCACCTGCAACCTCTTCATCCCCATCAACAAACCCTCAATCCCTCGCTTCTCCTTCAAACCTTGCTTCTGCTACTCAACCGAGTCCAACTCAGTCCAATCCGATAGCCTCAAAACTCTCGAATGGAACTCCATATGCAAGCAACTCTCAGCATTCACTTCCACTTCAATGGGCTCCTCCGCCGCCAACAACGCCCGTTTACCCGTCGGACGTACTCCAACAGACAGCCAAAAGCTTCTCGATCAAACCTCCGCTGCCAGACTCATTCCTCAACAACAACTCGACTTTTCTGGAATACATGACTTGACTGACATTCTCTCCGTTGCTGTTTCCGGTAGATTGTTGACTATTCCCGAGCTTTGCACCGTTCGAAGAACACTCGCTGCTTCCAGGGAATTGTTTGATAGTTTGAAACACTTGGCTTCTGTATCTAATCATTCACACAGGTTTGAACTTGGAATCTAA

Protein sequence

>MS.gene074598.t1

QRVTTPMQLTCNLFIPINKPSIPRFSFKPCFCYSTESNSVQSDSLKTLEWNSICKQLSAFTSTSMGSSAANNARLPVGRTPTDSQKLLDQTSAARLIPQQQLDFSGIHDLTDILSVAVSGRLLTIPELCTVRRTLAASRELFDSLKHLASVSNHSHRFELGI