Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059218.t1 | XP_024638078.1 | 99 | 397 | 4 | 0 | 1 | 397 | 1 | 397 | 6.10E-239 | 836.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059218.t1 | Q9SLN5 | 83.1 | 396 | 66 | 1 | 1 | 395 | 1 | 396 | 1.4e-204 | 713.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059218.t1 | A0A396I104 | 99.0 | 397 | 4 | 0 | 1 | 397 | 1 | 397 | 4.4e-239 | 836.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059218 | MS.gene45850 | 0.988523 | 2.37E-174 | -1.69E-46 |
| MS.gene059218 | MS.gene48925 | 0.987204 | 2.02E-169 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93411 | MS.gene059218 | PPI |
| MS.gene064281 | MS.gene059218 | PPI |
| MS.gene044411 | MS.gene059218 | PPI |
| MS.gene91353 | MS.gene059218 | PPI |
| MS.gene028573 | MS.gene059218 | PPI |
| MS.gene93798 | MS.gene059218 | PPI |
| MS.gene033096 | MS.gene059218 | PPI |
| MS.gene58403 | MS.gene059218 | PPI |
| MS.gene40160 | MS.gene059218 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059218.t1 | MTR_5g017630 | 49.470 | 283 | 137 | 2 | 100 | 382 | 73 | 349 | 2.26e-92 | 281 |
| MS.gene059218.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 78 | 354 | 6.89e-88 | 270 |
| MS.gene059218.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 54 | 330 | 1.17e-87 | 269 |
| MS.gene059218.t1 | MTR_5g017630 | 48.945 | 237 | 115 | 2 | 100 | 336 | 73 | 303 | 3.96e-72 | 228 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059218.t1 | AT2G45240 | 82.872 | 397 | 67 | 1 | 1 | 396 | 1 | 397 | 0.0 | 704 |
| MS.gene059218.t1 | AT2G45240 | 80.685 | 321 | 61 | 1 | 1 | 320 | 1 | 321 | 0.0 | 559 |
| MS.gene059218.t1 | AT1G13270 | 48.763 | 283 | 139 | 2 | 100 | 382 | 91 | 367 | 2.07e-93 | 285 |
| MS.gene059218.t1 | AT4G37040 | 50.177 | 283 | 135 | 3 | 100 | 382 | 72 | 348 | 3.59e-91 | 278 |
| MS.gene059218.t1 | AT3G25740 | 45.196 | 281 | 143 | 4 | 105 | 382 | 69 | 341 | 5.88e-82 | 254 |
| MS.gene059218.t1 | AT1G13270 | 44.949 | 198 | 104 | 1 | 100 | 297 | 91 | 283 | 4.77e-55 | 183 |
| MS.gene059218.t1 | AT3G25740 | 43.333 | 120 | 59 | 2 | 105 | 222 | 69 | 181 | 6.91e-24 | 100 |
Find 110 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTACGTGCAGCAAGATTT+TGG | 0.169908 | 4.1:-73248619 | None:intergenic |
| CCCGTGAAGGTTCGGCATTC+TGG | 0.238200 | 4.1:+73245512 | MS.gene059218:CDS |
| GCACCAAACATTCCTCATTA+TGG | 0.269416 | 4.1:+73249991 | MS.gene059218:CDS |
| CATAATTAGGTGACAGAAAC+TGG | 0.285298 | 4.1:+73251834 | MS.gene059218:intron |
| TTTCTCTCATTCTTTCAATC+TGG | 0.288753 | 4.1:-73247721 | None:intergenic |
| ATAACTATTGGGCTCATCTT+TGG | 0.289295 | 4.1:-73247564 | None:intergenic |
| CAAAGCTTCCTTACTTTGAT+TGG | 0.291250 | 4.1:+73247092 | MS.gene059218:CDS |
| TCAATTTGAGCATACTCTTT+TGG | 0.293472 | 4.1:+73250579 | MS.gene059218:CDS |
| CTCATTCTTTCAATCTGGTC+TGG | 0.298030 | 4.1:-73247716 | None:intergenic |
| AGCACAAGAGAGTTTATTTA+CGG | 0.299141 | 4.1:-73244801 | None:intergenic |
| AATACATACTGAAGATTTGC+AGG | 0.300572 | 4.1:-73244836 | None:intergenic |
| GACAATCCGTTTACCAGATA+TGG | 0.303126 | 4.1:-73247351 | None:intergenic |
| AGCTTCAATTCCATGCATTT+CGG | 0.337719 | 4.1:-73245487 | None:intergenic |
| TCATATTGTGGTCATGGAAT+TGG | 0.341090 | 4.1:+73249952 | MS.gene059218:CDS |
| CATATACCTGCATTGATCAT+TGG | 0.346647 | 4.1:-73250419 | None:intergenic |
| CTCATATTGTTATACCTGAT+TGG | 0.347912 | 4.1:+73247378 | MS.gene059218:CDS |
| TAACTATTGGGCTCATCTTT+GGG | 0.350866 | 4.1:-73247563 | None:intergenic |
| CTTCCATAATGAGGAATGTT+TGG | 0.353164 | 4.1:-73249994 | None:intergenic |
| AGTATGCTGAAGATAACTAT+TGG | 0.372630 | 4.1:-73247576 | None:intergenic |
| CGTTTACCAGATATGGGATA+TGG | 0.372750 | 4.1:-73247344 | None:intergenic |
| GGGGTACGATTCCGTGAAAT+TGG | 0.386841 | 4.1:+73249774 | MS.gene059218:CDS |
| ATACTGAAGATTTGCAGGTT+TGG | 0.398456 | 4.1:-73244831 | None:intergenic |
| CTGCGGCCATATCCCATATC+TGG | 0.400640 | 4.1:+73247338 | MS.gene059218:CDS |
| AATCGTCATGCTTCAATGTC+AGG | 0.408917 | 4.1:+73249807 | MS.gene059218:CDS |
| CTTCCTTACTTTGATTGGAC+TGG | 0.410698 | 4.1:+73247097 | MS.gene059218:CDS |
| CAATCAAAGTAAGGAAGCTT+TGG | 0.411005 | 4.1:-73247091 | None:intergenic |
| TAATAACTTCGCCAATTTCA+CGG | 0.416122 | 4.1:-73249785 | None:intergenic |
| TCATATTGTTATACCTGATT+GGG | 0.422149 | 4.1:+73247379 | MS.gene059218:CDS |
| CTATCAAATTCAGTTAAACC+TGG | 0.424605 | 4.1:+73249753 | MS.gene059218:intron |
| CTCACATCATCGGCCCAATC+AGG | 0.431265 | 4.1:-73247392 | None:intergenic |
| GTGTACTATAAAGGTGTTCA+TGG | 0.440792 | 4.1:+73249213 | MS.gene059218:CDS |
| GTATGCTGAAGATAACTATT+GGG | 0.447741 | 4.1:-73247575 | None:intergenic |
| CACATATGAGTGCTTGGATA+AGG | 0.447878 | 4.1:+73249379 | MS.gene059218:CDS |
| GAAGCTTCCCCGTGAAGGTT+CGG | 0.449403 | 4.1:+73245504 | MS.gene059218:CDS |
| GATCATTGGTTCAATTGTAA+AGG | 0.450952 | 4.1:-73250405 | None:intergenic |
| AGATCGATCAACCTTGCATC+AGG | 0.454183 | 4.1:-73248751 | None:intergenic |
| CCAGAATGCCGAACCTTCAC+GGG | 0.456791 | 4.1:-73245512 | None:intergenic |
| CATATCCCATATCTGGTAAA+CGG | 0.459117 | 4.1:+73247345 | MS.gene059218:CDS |
| TTCCTTACTTTGATTGGACT+GGG | 0.461313 | 4.1:+73247098 | MS.gene059218:CDS |
| ACCAGAATGCCGAACCTTCA+CGG | 0.461900 | 4.1:-73245513 | None:intergenic |
| TCTCAATGATACATACTTTG+TGG | 0.473493 | 4.1:+73249319 | MS.gene059218:CDS |
| CATCACCGAATGTTTATCCT+TGG | 0.478072 | 4.1:+73251886 | MS.gene059218:CDS |
| TTTAACCAAGGATAAACATT+CGG | 0.478433 | 4.1:-73251891 | None:intergenic |
| CTCAATGATACATACTTTGT+GGG | 0.479150 | 4.1:+73249320 | MS.gene059218:CDS |
| TACCCAGTCCAATCAAAGTA+AGG | 0.487570 | 4.1:-73247100 | None:intergenic |
| GTAAAATCATATTGTGGTCA+TGG | 0.489624 | 4.1:+73249946 | MS.gene059218:CDS |
| ACAATCCGTTTACCAGATAT+GGG | 0.495773 | 4.1:-73247350 | None:intergenic |
| AAATGGTAATTGAGAGGCGA+TGG | 0.498148 | 4.1:-73248592 | None:intergenic |
| GTAGATTGGAAGGGAGGTGT+TGG | 0.501126 | 4.1:+73248302 | MS.gene059218:intron |
| TCAGGTATAACAATATGAGC+TGG | 0.509419 | 4.1:-73247374 | None:intergenic |
| CACGAGGCAACTATTGCTGC+AGG | 0.513450 | 4.1:+73248384 | MS.gene059218:CDS |
| TATCAAATTCAGTTAAACCT+GGG | 0.516961 | 4.1:+73249754 | MS.gene059218:intron |
| CTGGCGTGATCGACTGTGGC+CGG | 0.519748 | 4.1:+73250516 | MS.gene059218:CDS |
| GTTAATGAAGTTATCTGCCA+TGG | 0.522636 | 4.1:+73248724 | MS.gene059218:CDS |
| GATGTTACCGTGTACTATAA+AGG | 0.523310 | 4.1:+73249204 | MS.gene059218:CDS |
| CCCAATTATGAATTTAACCA+AGG | 0.537081 | 4.1:-73251903 | None:intergenic |
| ATCGACTGTGGCCGGATGGA+TGG | 0.541522 | 4.1:+73250524 | MS.gene059218:CDS |
| TGATTCGTAGATTGGAAGGG+AGG | 0.546142 | 4.1:+73248296 | MS.gene059218:intron |
| GCCGCTCGGATTATTCAGCC+TGG | 0.550022 | 4.1:+73248330 | MS.gene059218:CDS |
| CAGACTCACTTGGTGAAGGA+TGG | 0.556738 | 4.1:+73247035 | MS.gene059218:CDS |
| CAAATGCACATATGAGTGCT+TGG | 0.558213 | 4.1:+73249373 | MS.gene059218:CDS |
| AAGAATGAGAGAAACATGTC+GGG | 0.558510 | 4.1:+73247730 | MS.gene059218:CDS |
| AAATTGATTCGTAGATTGGA+AGG | 0.558774 | 4.1:+73248292 | MS.gene059218:intron |
| AGGTGTTGGATGCAGCCGCT+CGG | 0.560148 | 4.1:+73248316 | MS.gene059218:CDS |
| ATTGAACCAATGATCAATGC+AGG | 0.561070 | 4.1:+73250413 | MS.gene059218:CDS |
| TCTCAGGTGTCCGAAATGCA+TGG | 0.565416 | 4.1:+73245477 | MS.gene059218:intron |
| TAAAGCAGTTGGTGTGATGA+AGG | 0.565699 | 4.1:+73250373 | MS.gene059218:CDS |
| GGATAAACATTCGGTGATGT+CGG | 0.569817 | 4.1:-73251882 | None:intergenic |
| ATTTCACGGAATCGTACCCC+AGG | 0.570700 | 4.1:-73249771 | None:intergenic |
| GGGAAGAAATGGTAATTGAG+AGG | 0.572534 | 4.1:-73248598 | None:intergenic |
| CAAAGGATACTTCCATAATG+AGG | 0.573541 | 4.1:-73250003 | None:intergenic |
| GTAACAGCAGTCCATCCATC+CGG | 0.573843 | 4.1:-73250535 | None:intergenic |
| GGTTGTACTGCGTGAAGAGA+GGG | 0.576273 | 4.1:+73247056 | MS.gene059218:CDS |
| CAGGTATAACAATATGAGCT+GGG | 0.581087 | 4.1:-73247373 | None:intergenic |
| AAAGAATGAGAGAAACATGT+CGG | 0.582562 | 4.1:+73247729 | MS.gene059218:CDS |
| GCACAGAACTCAGACTCACT+TGG | 0.583214 | 4.1:+73247025 | MS.gene059218:CDS |
| AATGGTAATTGAGAGGCGAT+GGG | 0.584764 | 4.1:-73248591 | None:intergenic |
| AAGGCAAAGGTATCCTCAGT+TGG | 0.584987 | 4.1:+73247001 | MS.gene059218:CDS |
| CGTGATCGACTGTGGCCGGA+TGG | 0.586768 | 4.1:+73250520 | MS.gene059218:CDS |
| GCCATGGAATTCCTGATGCA+AGG | 0.587828 | 4.1:+73248740 | MS.gene059218:CDS |
| ATTTCATCAGTTGTCACACC+AGG | 0.591424 | 4.1:-73248348 | None:intergenic |
| AACTCAGACTCACTTGGTGA+AGG | 0.594385 | 4.1:+73247031 | MS.gene059218:CDS |
| TACTGCGTGAAGAGAGGGCA+GGG | 0.594429 | 4.1:+73247061 | MS.gene059218:CDS |
| GCAGTTGGTGTGATGAAGGC+TGG | 0.599210 | 4.1:+73250377 | MS.gene059218:CDS |
| TTATCTTCAGCATACTGTTG+AGG | 0.599339 | 4.1:+73247583 | MS.gene059218:CDS |
| TTCTACAGGAAGCTCGAGGA+TGG | 0.599342 | 4.1:+73249165 | MS.gene059218:intron |
| GTACTGCGTGAAGAGAGGGC+AGG | 0.599704 | 4.1:+73247060 | MS.gene059218:CDS |
| TGGTTGTACTGCGTGAAGAG+AGG | 0.601052 | 4.1:+73247055 | MS.gene059218:CDS |
| TGGACTGCTGTTACAGCAGA+TGG | 0.605860 | 4.1:+73250544 | MS.gene059218:CDS |
| ATCAGTGCATCTGAAGGCAA+AGG | 0.606762 | 4.1:+73246988 | MS.gene059218:CDS |
| GCACAAATCAGTGCATCTGA+AGG | 0.608305 | 4.1:+73246982 | MS.gene059218:CDS |
| AGATGAAGAATCTCGTCAGT+TGG | 0.609382 | 4.1:+73249349 | MS.gene059218:CDS |
| TGAGTTCTGTGCGCCAACTG+AGG | 0.619133 | 4.1:-73247014 | None:intergenic |
| CTGGCAGGAAATAAAGCAGT+TGG | 0.619503 | 4.1:+73250362 | MS.gene059218:intron |
| ATCAAATTCAGTTAAACCTG+GGG | 0.627357 | 4.1:+73249755 | MS.gene059218:intron |
| AATTGATTCGTAGATTGGAA+GGG | 0.630358 | 4.1:+73248293 | MS.gene059218:intron |
| GAATTGAAGCTTCCCCGTGA+AGG | 0.631728 | 4.1:+73245499 | MS.gene059218:CDS |
| AGTCAGATAACTCACATCAT+CGG | 0.632480 | 4.1:-73247402 | None:intergenic |
| TTGGATAAGGCAATATCCAT+CGG | 0.639566 | 4.1:+73249392 | MS.gene059218:CDS |
| GTCATTCTACAGGAAGCTCG+AGG | 0.647941 | 4.1:+73249161 | MS.gene059218:intron |
| GAGGAATGTTTGGTGCACAA+TGG | 0.651672 | 4.1:-73249984 | None:intergenic |
| TTACAGGTAAAATCATATTG+TGG | 0.652206 | 4.1:+73249940 | MS.gene059218:intron |
| ATGAACACCTTTATAGTACA+CGG | 0.658395 | 4.1:-73249211 | None:intergenic |
| ACCTTGCATCAGGAATTCCA+TGG | 0.669846 | 4.1:-73248741 | None:intergenic |
| GTGTCTGGCGTGATCGACTG+TGG | 0.700621 | 4.1:+73250512 | MS.gene059218:CDS |
| CAGAATGCCGAACCTTCACG+GGG | 0.704929 | 4.1:-73245511 | None:intergenic |
| TTCAATGTCAGGCTTCTCAG+TGG | 0.712844 | 4.1:+73249818 | MS.gene059218:CDS |
| CTGTTCTTGCAAGGGCACTG+CGG | 0.721321 | 4.1:+73247321 | MS.gene059218:intron |
| ACCAGGCTGAATAATCCGAG+CGG | 0.746152 | 4.1:-73248331 | None:intergenic |
| AATTGACAGAGTAGTTCACG+AGG | 0.767513 | 4.1:+73248368 | MS.gene059218:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 73244779 | 73251920 | 73244779 | ID=MS.gene059218 |
| chr4.1 | mRNA | 73244779 | 73251920 | 73244779 | ID=MS.gene059218.t1;Parent=MS.gene059218 |
| chr4.1 | exon | 73244779 | 73244849 | 73244779 | ID=MS.gene059218.t1.exon1;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73244779 | 73244849 | 73244779 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73245483 | 73245533 | 73245483 | ID=MS.gene059218.t1.exon2;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73245483 | 73245533 | 73245483 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73246949 | 73247119 | 73246949 | ID=MS.gene059218.t1.exon3;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73246949 | 73247119 | 73246949 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73247334 | 73247409 | 73247334 | ID=MS.gene059218.t1.exon4;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73247334 | 73247409 | 73247334 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73247557 | 73247604 | 73247557 | ID=MS.gene059218.t1.exon5;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73247557 | 73247604 | 73247557 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73247707 | 73247751 | 73247707 | ID=MS.gene059218.t1.exon6;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73247707 | 73247751 | 73247707 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73248306 | 73248405 | 73248306 | ID=MS.gene059218.t1.exon7;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73248306 | 73248405 | 73248306 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73248584 | 73248635 | 73248584 | ID=MS.gene059218.t1.exon8;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73248584 | 73248635 | 73248584 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73248720 | 73248761 | 73248720 | ID=MS.gene059218.t1.exon9;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73248720 | 73248761 | 73248720 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73249173 | 73249234 | 73249173 | ID=MS.gene059218.t1.exon10;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73249173 | 73249234 | 73249173 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73249315 | 73249413 | 73249315 | ID=MS.gene059218.t1.exon11;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73249315 | 73249413 | 73249315 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73249766 | 73249839 | 73249766 | ID=MS.gene059218.t1.exon12;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73249766 | 73249839 | 73249766 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73249946 | 73250015 | 73249946 | ID=MS.gene059218.t1.exon13;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73249946 | 73250015 | 73249946 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73250369 | 73250434 | 73250369 | ID=MS.gene059218.t1.exon14;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73250369 | 73250434 | 73250369 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73250512 | 73250600 | 73250512 | ID=MS.gene059218.t1.exon15;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73250512 | 73250600 | 73250512 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| chr4.1 | exon | 73251843 | 73251920 | 73251843 | ID=MS.gene059218.t1.exon16;Parent=MS.gene059218.t1 |
| chr4.1 | CDS | 73251843 | 73251920 | 73251843 | ID=cds.MS.gene059218.t1;Parent=MS.gene059218.t1 |
| Gene Sequence |
| Protein sequence |
