Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48925.t1 | XP_024638078.1 | 99 | 397 | 4 | 0 | 1 | 397 | 1 | 397 | 6.10E-239 | 836.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48925.t1 | Q9SLN5 | 83.1 | 396 | 66 | 1 | 1 | 395 | 1 | 396 | 1.4e-204 | 713.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48925.t1 | A0A396I104 | 99.0 | 397 | 4 | 0 | 1 | 397 | 1 | 397 | 4.4e-239 | 836.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059218 | MS.gene48925 | 0.987204 | 2.02E-169 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044411 | MS.gene48925 | PPI |
| MS.gene48925 | MS.gene028573 | PPI |
| MS.gene48925 | MS.gene064281 | PPI |
| MS.gene48925 | MS.gene58403 | PPI |
| MS.gene93798 | MS.gene48925 | PPI |
| MS.gene48925 | MS.gene40160 | PPI |
| MS.gene48925 | MS.gene91353 | PPI |
| MS.gene48925 | MS.gene93411 | PPI |
| MS.gene48925 | MS.gene033096 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48925.t1 | MTR_5g017630 | 49.470 | 283 | 137 | 2 | 100 | 382 | 73 | 349 | 2.26e-92 | 281 |
| MS.gene48925.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 78 | 354 | 6.89e-88 | 270 |
| MS.gene48925.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 54 | 330 | 1.17e-87 | 269 |
| MS.gene48925.t1 | MTR_5g017630 | 48.945 | 237 | 115 | 2 | 100 | 336 | 73 | 303 | 3.96e-72 | 228 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48925.t1 | AT2G45240 | 82.872 | 397 | 67 | 1 | 1 | 396 | 1 | 397 | 0.0 | 704 |
| MS.gene48925.t1 | AT2G45240 | 80.685 | 321 | 61 | 1 | 1 | 320 | 1 | 321 | 0.0 | 559 |
| MS.gene48925.t1 | AT1G13270 | 48.763 | 283 | 139 | 2 | 100 | 382 | 91 | 367 | 2.07e-93 | 285 |
| MS.gene48925.t1 | AT4G37040 | 50.177 | 283 | 135 | 3 | 100 | 382 | 72 | 348 | 3.59e-91 | 278 |
| MS.gene48925.t1 | AT3G25740 | 45.196 | 281 | 143 | 4 | 105 | 382 | 69 | 341 | 5.88e-82 | 254 |
| MS.gene48925.t1 | AT1G13270 | 44.949 | 198 | 104 | 1 | 100 | 297 | 91 | 283 | 4.77e-55 | 183 |
| MS.gene48925.t1 | AT3G25740 | 43.333 | 120 | 59 | 2 | 105 | 222 | 69 | 181 | 6.91e-24 | 100 |
Find 110 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTACGTGCAGCAAGATTT+TGG | 0.169908 | 4.3:-72898907 | None:intergenic |
| CCCGTGAAGGTTCGGCATTC+TGG | 0.238200 | 4.3:+72895800 | MS.gene48925:CDS |
| GCACCAAACATTCCTCATTA+TGG | 0.269416 | 4.3:+72900278 | MS.gene48925:CDS |
| CATAATTAGGTGACAGAAAC+TGG | 0.285298 | 4.3:+72902121 | MS.gene48925:intron |
| TTTCTCTCATTCTTTCAATC+TGG | 0.288753 | 4.3:-72898009 | None:intergenic |
| ATAACTATTGGGCTCATCTT+TGG | 0.289295 | 4.3:-72897852 | None:intergenic |
| CAAAGCTTCCTTACTTTGAT+TGG | 0.291250 | 4.3:+72897380 | MS.gene48925:CDS |
| TCAATTTGAGCATACTCTTT+TGG | 0.293472 | 4.3:+72900867 | MS.gene48925:CDS |
| CTCATTCTTTCAATCTGGTC+TGG | 0.298030 | 4.3:-72898004 | None:intergenic |
| AGCACAAGAGAGTTTATTTA+CGG | 0.299141 | 4.3:-72895087 | None:intergenic |
| AATACATACTGAAGATTTGC+AGG | 0.300572 | 4.3:-72895122 | None:intergenic |
| GACAATCCGTTTACCAGATA+TGG | 0.303126 | 4.3:-72897639 | None:intergenic |
| AGCTTCAATTCCATGCATTT+CGG | 0.337719 | 4.3:-72895775 | None:intergenic |
| TCATATTGTGGTCATGGAAT+TGG | 0.341090 | 4.3:+72900239 | MS.gene48925:CDS |
| CATATACCTGCATTGATCAT+TGG | 0.346647 | 4.3:-72900707 | None:intergenic |
| CTCATATTGTTATACCTGAT+TGG | 0.347912 | 4.3:+72897666 | MS.gene48925:CDS |
| TAACTATTGGGCTCATCTTT+GGG | 0.350866 | 4.3:-72897851 | None:intergenic |
| CTTCCATAATGAGGAATGTT+TGG | 0.353164 | 4.3:-72900281 | None:intergenic |
| AGTATGCTGAAGATAACTAT+TGG | 0.372630 | 4.3:-72897864 | None:intergenic |
| CGTTTACCAGATATGGGATA+TGG | 0.372750 | 4.3:-72897632 | None:intergenic |
| GGGGTACGATTCCGTGAAAT+TGG | 0.386841 | 4.3:+72900062 | MS.gene48925:CDS |
| ATACTGAAGATTTGCAGGTT+TGG | 0.398456 | 4.3:-72895117 | None:intergenic |
| CTGCGGCCATATCCCATATC+TGG | 0.400640 | 4.3:+72897626 | MS.gene48925:CDS |
| AATCGTCATGCTTCAATGTC+AGG | 0.408917 | 4.3:+72900095 | MS.gene48925:CDS |
| CTTCCTTACTTTGATTGGAC+TGG | 0.410698 | 4.3:+72897385 | MS.gene48925:CDS |
| CAATCAAAGTAAGGAAGCTT+TGG | 0.411005 | 4.3:-72897379 | None:intergenic |
| TAATAACTTCGCCAATTTCA+CGG | 0.416122 | 4.3:-72900073 | None:intergenic |
| TCATATTGTTATACCTGATT+GGG | 0.422149 | 4.3:+72897667 | MS.gene48925:CDS |
| CTATCAAATTCAGTTAAACC+TGG | 0.424605 | 4.3:+72900041 | MS.gene48925:intron |
| CTCACATCATCGGCCCAATC+AGG | 0.431265 | 4.3:-72897680 | None:intergenic |
| GTGTACTATAAAGGTGTTCA+TGG | 0.440792 | 4.3:+72899501 | MS.gene48925:CDS |
| GTATGCTGAAGATAACTATT+GGG | 0.447741 | 4.3:-72897863 | None:intergenic |
| CACATATGAGTGCTTGGATA+AGG | 0.447878 | 4.3:+72899667 | MS.gene48925:CDS |
| GAAGCTTCCCCGTGAAGGTT+CGG | 0.449403 | 4.3:+72895792 | MS.gene48925:CDS |
| GATCATTGGTTCAATTGTAA+AGG | 0.450952 | 4.3:-72900693 | None:intergenic |
| AGATCGATCAACCTTGCATC+AGG | 0.454183 | 4.3:-72899039 | None:intergenic |
| CCAGAATGCCGAACCTTCAC+GGG | 0.456791 | 4.3:-72895800 | None:intergenic |
| CATATCCCATATCTGGTAAA+CGG | 0.459117 | 4.3:+72897633 | MS.gene48925:CDS |
| TTCCTTACTTTGATTGGACT+GGG | 0.461313 | 4.3:+72897386 | MS.gene48925:CDS |
| ACCAGAATGCCGAACCTTCA+CGG | 0.461900 | 4.3:-72895801 | None:intergenic |
| TCTCAATGATACATACTTTG+TGG | 0.473493 | 4.3:+72899607 | MS.gene48925:CDS |
| CATCACCGAATGTTTATCCT+TGG | 0.478072 | 4.3:+72902173 | MS.gene48925:CDS |
| TTTAACCAAGGATAAACATT+CGG | 0.478433 | 4.3:-72902178 | None:intergenic |
| CTCAATGATACATACTTTGT+GGG | 0.479150 | 4.3:+72899608 | MS.gene48925:CDS |
| TACCCAGTCCAATCAAAGTA+AGG | 0.487570 | 4.3:-72897388 | None:intergenic |
| GTAAAATCATATTGTGGTCA+TGG | 0.489624 | 4.3:+72900233 | MS.gene48925:CDS |
| ACAATCCGTTTACCAGATAT+GGG | 0.495773 | 4.3:-72897638 | None:intergenic |
| AAATGGTAATTGAGAGGCGA+TGG | 0.498148 | 4.3:-72898880 | None:intergenic |
| GTAGATTGGAAGGGAGGTGT+TGG | 0.501126 | 4.3:+72898590 | MS.gene48925:intron |
| TCAGGTATAACAATATGAGC+TGG | 0.509419 | 4.3:-72897662 | None:intergenic |
| CACGAGGCAACTATTGCTGC+AGG | 0.513450 | 4.3:+72898672 | MS.gene48925:CDS |
| TATCAAATTCAGTTAAACCT+GGG | 0.516961 | 4.3:+72900042 | MS.gene48925:intron |
| CTGGCGTGATCGACTGTGGC+CGG | 0.519748 | 4.3:+72900804 | MS.gene48925:CDS |
| GTTAATGAAGTTATCTGCCA+TGG | 0.522636 | 4.3:+72899012 | MS.gene48925:CDS |
| GATGTTACCGTGTACTATAA+AGG | 0.523310 | 4.3:+72899492 | MS.gene48925:CDS |
| CCCAATTATGAATTTAACCA+AGG | 0.537081 | 4.3:-72902190 | None:intergenic |
| ATCGACTGTGGCCGGATGGA+TGG | 0.541522 | 4.3:+72900812 | MS.gene48925:CDS |
| TGATTCGTAGATTGGAAGGG+AGG | 0.546142 | 4.3:+72898584 | MS.gene48925:intron |
| GCCGCTCGGATTATTCAGCC+TGG | 0.550022 | 4.3:+72898618 | MS.gene48925:CDS |
| AAATTGATTCGTAGATTGGA+AGG | 0.550825 | 4.3:+72898580 | MS.gene48925:intron |
| CAGACTCACTTGGTGAAGGA+TGG | 0.556738 | 4.3:+72897323 | MS.gene48925:CDS |
| CAAATGCACATATGAGTGCT+TGG | 0.558213 | 4.3:+72899661 | MS.gene48925:CDS |
| AAGAATGAGAGAAACATGTC+GGG | 0.558510 | 4.3:+72898018 | MS.gene48925:CDS |
| AGGTGTTGGATGCAGCCGCT+CGG | 0.560148 | 4.3:+72898604 | MS.gene48925:CDS |
| ATTGAACCAATGATCAATGC+AGG | 0.561070 | 4.3:+72900701 | MS.gene48925:CDS |
| TCTCAGGTGTCCGAAATGCA+TGG | 0.565416 | 4.3:+72895765 | MS.gene48925:intron |
| TAAAGCAGTTGGTGTGATGA+AGG | 0.565699 | 4.3:+72900661 | MS.gene48925:CDS |
| GGATAAACATTCGGTGATGT+CGG | 0.569817 | 4.3:-72902169 | None:intergenic |
| ATTTCACGGAATCGTACCCC+AGG | 0.570700 | 4.3:-72900059 | None:intergenic |
| GGGAAGAAATGGTAATTGAG+AGG | 0.572534 | 4.3:-72898886 | None:intergenic |
| CAAAGGATACTTCCATAATG+AGG | 0.573541 | 4.3:-72900290 | None:intergenic |
| GTAACAGCAGTCCATCCATC+CGG | 0.573843 | 4.3:-72900823 | None:intergenic |
| GGTTGTACTGCGTGAAGAGA+GGG | 0.576273 | 4.3:+72897344 | MS.gene48925:CDS |
| CAGGTATAACAATATGAGCT+GGG | 0.581087 | 4.3:-72897661 | None:intergenic |
| AAAGAATGAGAGAAACATGT+CGG | 0.582562 | 4.3:+72898017 | MS.gene48925:CDS |
| GCACAGAACTCAGACTCACT+TGG | 0.583214 | 4.3:+72897313 | MS.gene48925:CDS |
| AATGGTAATTGAGAGGCGAT+GGG | 0.584764 | 4.3:-72898879 | None:intergenic |
| AAGGCAAAGGTATCCTCAGT+TGG | 0.584987 | 4.3:+72897289 | MS.gene48925:CDS |
| CGTGATCGACTGTGGCCGGA+TGG | 0.586768 | 4.3:+72900808 | MS.gene48925:CDS |
| GCCATGGAATTCCTGATGCA+AGG | 0.587828 | 4.3:+72899028 | MS.gene48925:CDS |
| ATTTCATCAGTTGTCACACC+AGG | 0.591424 | 4.3:-72898636 | None:intergenic |
| AACTCAGACTCACTTGGTGA+AGG | 0.594385 | 4.3:+72897319 | MS.gene48925:CDS |
| TACTGCGTGAAGAGAGGGCA+GGG | 0.594429 | 4.3:+72897349 | MS.gene48925:CDS |
| GCAGTTGGTGTGATGAAGGC+TGG | 0.599210 | 4.3:+72900665 | MS.gene48925:CDS |
| TTATCTTCAGCATACTGTTG+AGG | 0.599339 | 4.3:+72897871 | MS.gene48925:CDS |
| TTCTACAGGAAGCTCGAGGA+TGG | 0.599342 | 4.3:+72899453 | MS.gene48925:intron |
| GTACTGCGTGAAGAGAGGGC+AGG | 0.599704 | 4.3:+72897348 | MS.gene48925:CDS |
| TGGTTGTACTGCGTGAAGAG+AGG | 0.601052 | 4.3:+72897343 | MS.gene48925:CDS |
| TGGACTGCTGTTACAGCAGA+TGG | 0.605860 | 4.3:+72900832 | MS.gene48925:CDS |
| ATCAGTGCATCTGAAGGCAA+AGG | 0.606762 | 4.3:+72897276 | MS.gene48925:CDS |
| GCACAAATCAGTGCATCTGA+AGG | 0.608305 | 4.3:+72897270 | MS.gene48925:CDS |
| AGATGAAGAATCTCGTCAGT+TGG | 0.609382 | 4.3:+72899637 | MS.gene48925:CDS |
| TGAGTTCTGTGCGCCAACTG+AGG | 0.619133 | 4.3:-72897302 | None:intergenic |
| CTGGCAGGAAATAAAGCAGT+TGG | 0.619503 | 4.3:+72900650 | MS.gene48925:intron |
| ATCAAATTCAGTTAAACCTG+GGG | 0.627357 | 4.3:+72900043 | MS.gene48925:intron |
| AATTGATTCGTAGATTGGAA+GGG | 0.627919 | 4.3:+72898581 | MS.gene48925:intron |
| GAATTGAAGCTTCCCCGTGA+AGG | 0.631728 | 4.3:+72895787 | MS.gene48925:CDS |
| AGTCAGATAACTCACATCAT+CGG | 0.632480 | 4.3:-72897690 | None:intergenic |
| TTGGATAAGGCAATATCCAT+CGG | 0.639566 | 4.3:+72899680 | MS.gene48925:CDS |
| GTCATTCTACAGGAAGCTCG+AGG | 0.647941 | 4.3:+72899449 | MS.gene48925:intron |
| GAGGAATGTTTGGTGCACAA+TGG | 0.651672 | 4.3:-72900271 | None:intergenic |
| TTACAGGTAAAATCATATTG+TGG | 0.652206 | 4.3:+72900227 | MS.gene48925:intron |
| ATGAACACCTTTATAGTACA+CGG | 0.658395 | 4.3:-72899499 | None:intergenic |
| ACCTTGCATCAGGAATTCCA+TGG | 0.669846 | 4.3:-72899029 | None:intergenic |
| GTGTCTGGCGTGATCGACTG+TGG | 0.700621 | 4.3:+72900800 | MS.gene48925:CDS |
| CAGAATGCCGAACCTTCACG+GGG | 0.704929 | 4.3:-72895799 | None:intergenic |
| TTCAATGTCAGGCTTCTCAG+TGG | 0.712844 | 4.3:+72900106 | MS.gene48925:CDS |
| CTGTTCTTGCAAGGGCACTG+CGG | 0.721321 | 4.3:+72897609 | MS.gene48925:intron |
| ACCAGGCTGAATAATCCGAG+CGG | 0.746152 | 4.3:-72898619 | None:intergenic |
| AATTGACAGAGTAGTTCACG+AGG | 0.767513 | 4.3:+72898656 | MS.gene48925:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 72895065 | 72902207 | 72895065 | ID=MS.gene48925 |
| chr4.3 | mRNA | 72895065 | 72902207 | 72895065 | ID=MS.gene48925.t1;Parent=MS.gene48925 |
| chr4.3 | exon | 72895065 | 72895135 | 72895065 | ID=MS.gene48925.t1.exon1;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72895065 | 72895135 | 72895065 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72895771 | 72895821 | 72895771 | ID=MS.gene48925.t1.exon2;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72895771 | 72895821 | 72895771 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72897237 | 72897407 | 72897237 | ID=MS.gene48925.t1.exon3;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72897237 | 72897407 | 72897237 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72897622 | 72897697 | 72897622 | ID=MS.gene48925.t1.exon4;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72897622 | 72897697 | 72897622 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72897845 | 72897892 | 72897845 | ID=MS.gene48925.t1.exon5;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72897845 | 72897892 | 72897845 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72897995 | 72898039 | 72897995 | ID=MS.gene48925.t1.exon6;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72897995 | 72898039 | 72897995 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72898594 | 72898693 | 72898594 | ID=MS.gene48925.t1.exon7;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72898594 | 72898693 | 72898594 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72898872 | 72898923 | 72898872 | ID=MS.gene48925.t1.exon8;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72898872 | 72898923 | 72898872 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72899008 | 72899049 | 72899008 | ID=MS.gene48925.t1.exon9;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72899008 | 72899049 | 72899008 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72899461 | 72899522 | 72899461 | ID=MS.gene48925.t1.exon10;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72899461 | 72899522 | 72899461 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72899603 | 72899701 | 72899603 | ID=MS.gene48925.t1.exon11;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72899603 | 72899701 | 72899603 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72900054 | 72900127 | 72900054 | ID=MS.gene48925.t1.exon12;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72900054 | 72900127 | 72900054 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72900233 | 72900302 | 72900233 | ID=MS.gene48925.t1.exon13;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72900233 | 72900302 | 72900233 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72900657 | 72900722 | 72900657 | ID=MS.gene48925.t1.exon14;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72900657 | 72900722 | 72900657 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72900800 | 72900888 | 72900800 | ID=MS.gene48925.t1.exon15;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72900800 | 72900888 | 72900800 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| chr4.3 | exon | 72902130 | 72902207 | 72902130 | ID=MS.gene48925.t1.exon16;Parent=MS.gene48925.t1 |
| chr4.3 | CDS | 72902130 | 72902207 | 72902130 | ID=cds.MS.gene48925.t1;Parent=MS.gene48925.t1 |
| Gene Sequence |
| Protein sequence |
