Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05927.t1 | AET05287.1 | 92.7 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 4.00E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05927.t1 | Q54UU1 | 54.4 | 125 | 56 | 1 | 15 | 138 | 3 | 127 | 3.2e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05927.t1 | G7L8Y7 | 92.7 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 2.9e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96826 | MS.gene05927 | PPI |
| MS.gene59549 | MS.gene05927 | PPI |
| MS.gene031550 | MS.gene05927 | PPI |
| MS.gene006753 | MS.gene05927 | PPI |
| MS.gene85984 | MS.gene05927 | PPI |
| MS.gene068341 | MS.gene05927 | PPI |
| MS.gene05927 | MS.gene87621 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05927.t1 | MTR_8g103680 | 92.715 | 151 | 11 | 0 | 2 | 152 | 63 | 213 | 5.57e-105 | 298 |
| MS.gene05927.t1 | MTR_5g075330 | 87.333 | 150 | 19 | 0 | 3 | 152 | 25 | 174 | 3.74e-101 | 287 |
| MS.gene05927.t1 | MTR_5g075330 | 90.071 | 141 | 14 | 0 | 12 | 152 | 1 | 141 | 3.63e-98 | 279 |
| MS.gene05927.t1 | MTR_8g095450 | 28.873 | 142 | 84 | 3 | 18 | 143 | 28 | 168 | 1.03e-14 | 67.8 |
| MS.gene05927.t1 | MTR_4g048530 | 29.787 | 141 | 84 | 3 | 18 | 143 | 26 | 166 | 1.48e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05927.t1 | AT5G02280 | 87.943 | 141 | 17 | 0 | 12 | 152 | 1 | 141 | 4.05e-94 | 268 |
| MS.gene05927.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
| MS.gene05927.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
| MS.gene05927.t1 | AT1G51160 | 29.078 | 141 | 85 | 3 | 18 | 143 | 26 | 166 | 5.86e-14 | 65.9 |
Find 35 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGTTGTCACCTGTTTC+TGG | 0.050534 | 4.4:+2892997 | MS.gene05927:CDS |
| TTTCCTGATATTTAGGATTA+TGG | 0.113471 | 4.4:+2892901 | MS.gene05927:intron |
| ATTTAGGATTATGGGTCTTC+TGG | 0.243224 | 4.4:+2892910 | MS.gene05927:intron |
| CTTTCCATTTGTTGAGTTCC+AGG | 0.279445 | 4.4:-2893599 | None:intergenic |
| TTCCTGATATTTAGGATTAT+GGG | 0.283975 | 4.4:+2892902 | MS.gene05927:intron |
| CAGATTCTTGCAAAGGCTTT+GGG | 0.307507 | 4.4:+2892217 | MS.gene05927:CDS |
| TCAGATTCTTGCAAAGGCTT+TGG | 0.323137 | 4.4:+2892216 | MS.gene05927:CDS |
| GAAGGACCGTGTTGCATTGC+TGG | 0.349606 | 4.4:+2893739 | MS.gene05927:CDS |
| CGGTGGATTGATCTACTATA+AGG | 0.397918 | 4.4:+2892279 | MS.gene05927:CDS |
| AGGCATCTCCATCTCATAGA+AGG | 0.407862 | 4.4:-2893672 | None:intergenic |
| GCTTACGGGTGGCTAGCTTA+TGG | 0.427608 | 4.4:+2892953 | MS.gene05927:CDS |
| AAATAATACCTGTCAGTGAC+TGG | 0.443726 | 4.4:-2893071 | None:intergenic |
| ATTCCTGCGCAACCAGAAAC+AGG | 0.450165 | 4.4:-2893009 | None:intergenic |
| TGAACCTGGAACTCAACAAA+TGG | 0.475337 | 4.4:+2893595 | MS.gene05927:CDS |
| TCAAAGAGCTCACATCGTAT+AGG | 0.480843 | 4.4:-2893692 | None:intergenic |
| GGACACCAATGATAGCTTAC+GGG | 0.481121 | 4.4:+2892939 | MS.gene05927:CDS |
| CATTGCTTCCAGTCACTGAC+AGG | 0.492920 | 4.4:+2893063 | MS.gene05927:CDS |
| GGCATCTCCATCTCATAGAA+GGG | 0.506472 | 4.4:-2893671 | None:intergenic |
| GGCATTTCAGATTCTTGCAA+AGG | 0.507176 | 4.4:+2892210 | MS.gene05927:CDS |
| AGCCACCCGTAAGCTATCAT+TGG | 0.510288 | 4.4:-2892944 | None:intergenic |
| TGGACACCAATGATAGCTTA+CGG | 0.512682 | 4.4:+2892938 | MS.gene05927:CDS |
| GAAGAACCCCTTCTATGAGA+TGG | 0.515244 | 4.4:+2893664 | MS.gene05927:CDS |
| TCACCTGTTTCTGGTTGCGC+AGG | 0.516213 | 4.4:+2893006 | MS.gene05927:CDS |
| CTTTGATATCAACTTAGCTC+AGG | 0.519060 | 4.4:+2893709 | MS.gene05927:CDS |
| AAGGACCGTGTTGCATTGCT+GGG | 0.527169 | 4.4:+2893740 | MS.gene05927:CDS |
| GAAGATCAAATGTGTCAGCT+TGG | 0.530412 | 4.4:-2893041 | None:intergenic |
| CTTTACATCATCAATAAGTC+CGG | 0.534062 | 4.4:+2892259 | MS.gene05927:CDS |
| CTTAGCTCAGGCAGTGCAGA+AGG | 0.554879 | 4.4:+2893721 | MS.gene05927:CDS |
| TTATGGGTCTTCTGGACGAA+TGG | 0.565210 | 4.4:+2892918 | MS.gene05927:CDS |
| TACATCATCAATAAGTCCGG+TGG | 0.589559 | 4.4:+2892262 | MS.gene05927:CDS |
| AGGTGACAACTGCTGAGAGA+TGG | 0.604194 | 4.4:-2892989 | None:intergenic |
| GCATCTCCATCTCATAGAAG+GGG | 0.643808 | 4.4:-2893670 | None:intergenic |
| CACCAATGATAGCTTACGGG+TGG | 0.665422 | 4.4:+2892942 | MS.gene05927:CDS |
| CTTATAGTAGATCAATCCAC+CGG | 0.673585 | 4.4:-2892278 | None:intergenic |
| ATCGACCCAGCAATGCAACA+CGG | 0.698434 | 4.4:-2893745 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAATACTAAAAAATTT+AGG | + | chr4.4:2892527-2892546 | MS.gene05927:intron | 10.0% |
| !! | GAAAGTTTAGTAATAATTAT+TGG | + | chr4.4:2893292-2893311 | MS.gene05927:intron | 15.0% |
| !!! | TTATTTTTATTGTAAGCTTA+AGG | - | chr4.4:2892637-2892656 | None:intergenic | 15.0% |
| !! | CTTAACATAATAATGAGTTA+TGG | - | chr4.4:2893098-2893117 | None:intergenic | 20.0% |
| !!! | ACTCATTGATTTTTAGTTTT+AGG | + | chr4.4:2892563-2892582 | MS.gene05927:intron | 20.0% |
| !!! | TGATTTTTAGTTTTAGGATA+TGG | + | chr4.4:2892569-2892588 | MS.gene05927:intron | 20.0% |
| !!! | TGTTTAATTTCCTGATATTT+AGG | + | chr4.4:2892894-2892913 | MS.gene05927:intron | 20.0% |
| !!! | TTCAGTAAAACATTTTTCAT+AGG | - | chr4.4:2892375-2892394 | None:intergenic | 20.0% |
| ! | AAAAACTTTCTGAGTTAGAT+GGG | - | chr4.4:2892801-2892820 | None:intergenic | 25.0% |
| ! | GGTAAGAATTCAAACATAAT+TGG | - | chr4.4:2893399-2893418 | None:intergenic | 25.0% |
| ! | TAAAAACTTTCTGAGTTAGA+TGG | - | chr4.4:2892802-2892821 | None:intergenic | 25.0% |
| ! | TGTGAATAAAAAGCAAACAT+TGG | - | chr4.4:2893199-2893218 | None:intergenic | 25.0% |
| !! | AAAGTTTTTACTACTGTTGT+TGG | + | chr4.4:2892812-2892831 | MS.gene05927:intron | 25.0% |
| !! | ATTTAAAATCTACTAGTGCT+AGG | - | chr4.4:2893522-2893541 | None:intergenic | 25.0% |
| !! | TTCCTGATATTTAGGATTAT+GGG | + | chr4.4:2892902-2892921 | MS.gene05927:intron | 25.0% |
| !! | TTTCCTGATATTTAGGATTA+TGG | + | chr4.4:2892901-2892920 | MS.gene05927:intron | 25.0% |
| !!! | TGTTTGCTTTTTATTCACAT+TGG | + | chr4.4:2893200-2893219 | MS.gene05927:intron | 25.0% |
| AAGAATGAATGCCAAAAATC+TGG | + | chr4.4:2893145-2893164 | MS.gene05927:intron | 30.0% | |
| CAATTAAGGTGACAAATCTA+CGG | - | chr4.4:2893420-2893439 | None:intergenic | 30.0% | |
| CTTTACATCATCAATAAGTC+CGG | + | chr4.4:2892259-2892278 | MS.gene05927:CDS | 30.0% | |
| TACATACAGGTAAAATTCCA+AGG | - | chr4.4:2893335-2893354 | None:intergenic | 30.0% | |
| ! | AATAAGTTGGGTTGTTAATC+AGG | + | chr4.4:2892416-2892435 | MS.gene05927:intron | 30.0% |
| ! | AGTGATATGTACCAGATTTT+TGG | - | chr4.4:2893159-2893178 | None:intergenic | 30.0% |
| ! | CAAACATAATTGGTAACAAC+TGG | - | chr4.4:2893389-2893408 | None:intergenic | 30.0% |
| ! | CTGGGTTTTACTAACATTTA+AGG | + | chr4.4:2893266-2893285 | MS.gene05927:intron | 30.0% |
| AAATAATACCTGTCAGTGAC+TGG | - | chr4.4:2893074-2893093 | None:intergenic | 35.0% | |
| AATTAATTCTTCTCTGCCAC+AGG | + | chr4.4:2893551-2893570 | MS.gene05927:intron | 35.0% | |
| ACCCAACTTATTCATCAAAG+AGG | - | chr4.4:2892408-2892427 | None:intergenic | 35.0% | |
| ATCTGGTACATATCACTGAT+TGG | + | chr4.4:2893162-2893181 | MS.gene05927:intron | 35.0% | |
| ATTAATTCTTCTCTGCCACA+GGG | + | chr4.4:2893552-2893571 | MS.gene05927:intron | 35.0% | |
| CCCAACTTATTCATCAAAGA+GGG | - | chr4.4:2892407-2892426 | None:intergenic | 35.0% | |
| CCCTCTTTGATGAATAAGTT+GGG | + | chr4.4:2892404-2892423 | MS.gene05927:intron | 35.0% | |
| CGTAGATTTGTCACCTTAAT+TGG | + | chr4.4:2893418-2893437 | MS.gene05927:intron | 35.0% | |
| CTTATAGTAGATCAATCCAC+CGG | - | chr4.4:2892281-2892300 | None:intergenic | 35.0% | |
| CTTTGATATCAACTTAGCTC+AGG | + | chr4.4:2893709-2893728 | MS.gene05927:CDS | 35.0% | |
| GACCCATAATCCTAAATATC+AGG | - | chr4.4:2892907-2892926 | None:intergenic | 35.0% | |
| TACTAGTGCTAGGTTATAGA+TGG | - | chr4.4:2893512-2893531 | None:intergenic | 35.0% | |
| TAGTCTCTACTCTCTAATGT+GGG | + | chr4.4:2892329-2892348 | MS.gene05927:intron | 35.0% | |
| TCACGTCGTAGATGAAAATA+AGG | + | chr4.4:2892594-2892613 | MS.gene05927:intron | 35.0% | |
| TGTAATGCCTTGGTCATTTA+AGG | + | chr4.4:2893351-2893370 | MS.gene05927:intron | 35.0% | |
| TTAATCAGGTCACTTGAACT+TGG | + | chr4.4:2892430-2892449 | MS.gene05927:intron | 35.0% | |
| TTCACATTGGTAAGTAACCT+TGG | + | chr4.4:2893213-2893232 | MS.gene05927:intron | 35.0% | |
| TTTACCTGTATGTAATGCCT+TGG | + | chr4.4:2893341-2893360 | MS.gene05927:intron | 35.0% | |
| TTTGTCAAGGCTGTTAATCA+AGG | - | chr4.4:2892740-2892759 | None:intergenic | 35.0% | |
| ! | ATTTAGGATTATGGGTCTTC+TGG | + | chr4.4:2892910-2892929 | MS.gene05927:intron | 35.0% |
| !! | TAACATAGTCTTATGTGCCT+TGG | + | chr4.4:2893315-2893334 | MS.gene05927:intron | 35.0% |
| ACATTGGTAAGTAACCTTGG+AGG | + | chr4.4:2893216-2893235 | MS.gene05927:intron | 40.0% | |
| ACGCCATTGTGAATTTGTCA+AGG | - | chr4.4:2892753-2892772 | None:intergenic | 40.0% | |
| CAGATTCTTGCAAAGGCTTT+GGG | + | chr4.4:2892217-2892236 | MS.gene05927:CDS | 40.0% | |
| CAGCCTTGACAAATTCACAA+TGG | + | chr4.4:2892747-2892766 | MS.gene05927:intron | 40.0% | |
| CTAGTCTCTACTCTCTAATG+TGG | + | chr4.4:2892328-2892347 | MS.gene05927:intron | 40.0% | |
| GAAGATCAAATGTGTCAGCT+TGG | - | chr4.4:2893044-2893063 | None:intergenic | 40.0% | |
| GCCCTCTTTGATGAATAAGT+TGG | + | chr4.4:2892403-2892422 | MS.gene05927:intron | 40.0% | |
| GGCATTTCAGATTCTTGCAA+AGG | + | chr4.4:2892210-2892229 | MS.gene05927:CDS | 40.0% | |
| TACATCATCAATAAGTCCGG+TGG | + | chr4.4:2892262-2892281 | MS.gene05927:CDS | 40.0% | |
| TCAAAGAGCTCACATCGTAT+AGG | - | chr4.4:2893695-2893714 | None:intergenic | 40.0% | |
| TCAGATTCTTGCAAAGGCTT+TGG | + | chr4.4:2892216-2892235 | MS.gene05927:CDS | 40.0% | |
| TGAACCTGGAACTCAACAAA+TGG | + | chr4.4:2893595-2893614 | MS.gene05927:CDS | 40.0% | |
| TGGACACCAATGATAGCTTA+CGG | + | chr4.4:2892938-2892957 | MS.gene05927:CDS | 40.0% | |
| ! | ATGACCAAGGCATTACATAC+AGG | - | chr4.4:2893348-2893367 | None:intergenic | 40.0% |
| ! | CTTTCCATTTGTTGAGTTCC+AGG | - | chr4.4:2893602-2893621 | None:intergenic | 40.0% |
| ! | CTTTCTGAGTTAGATGGGTT+TGG | - | chr4.4:2892796-2892815 | None:intergenic | 40.0% |
| !! | CGGTGGATTGATCTACTATA+AGG | + | chr4.4:2892279-2892298 | MS.gene05927:CDS | 40.0% |
| AGGCATCTCCATCTCATAGA+AGG | - | chr4.4:2893675-2893694 | None:intergenic | 45.0% | |
| GAAGAACCCCTTCTATGAGA+TGG | + | chr4.4:2893664-2893683 | MS.gene05927:CDS | 45.0% | |
| GAGTAGAGACTAGAGAGCAA+TGG | - | chr4.4:2892321-2892340 | None:intergenic | 45.0% | |
| GCATCTCCATCTCATAGAAG+GGG | - | chr4.4:2893673-2893692 | None:intergenic | 45.0% | |
| GGACACCAATGATAGCTTAC+GGG | + | chr4.4:2892939-2892958 | MS.gene05927:CDS | 45.0% | |
| GGCATCTCCATCTCATAGAA+GGG | - | chr4.4:2893674-2893693 | None:intergenic | 45.0% | |
| GTGAACGCAAAGGCCAATTA+AGG | - | chr4.4:2893434-2893453 | None:intergenic | 45.0% | |
| TTATGGGTCTTCTGGACGAA+TGG | + | chr4.4:2892918-2892937 | MS.gene05927:CDS | 45.0% | |
| ! | CAAGGCACCTTAAATGACCA+AGG | - | chr4.4:2893361-2893380 | None:intergenic | 45.0% |
| ! | TTTTTCGTGGTCTGTGAACC+TGG | + | chr4.4:2893581-2893600 | MS.gene05927:CDS | 45.0% |
| AAGGACCGTGTTGCATTGCT+GGG | + | chr4.4:2893740-2893759 | MS.gene05927:CDS | 50.0% | |
| AGCCACCCGTAAGCTATCAT+TGG | - | chr4.4:2892947-2892966 | None:intergenic | 50.0% | |
| AGGTGACAACTGCTGAGAGA+TGG | - | chr4.4:2892992-2893011 | None:intergenic | 50.0% | |
| ATCGACCCAGCAATGCAACA+CGG | - | chr4.4:2893748-2893767 | None:intergenic | 50.0% | |
| ATTCCTGCGCAACCAGAAAC+AGG | - | chr4.4:2893012-2893031 | None:intergenic | 50.0% | |
| CACCAATGATAGCTTACGGG+TGG | + | chr4.4:2892942-2892961 | MS.gene05927:CDS | 50.0% | |
| CACGAAAAACTTCGTCCCTG+TGG | - | chr4.4:2893570-2893589 | None:intergenic | 50.0% | |
| CAGCAGTTGTCACCTGTTTC+TGG | + | chr4.4:2892997-2893016 | MS.gene05927:CDS | 50.0% | |
| CAGCATCACAGTGAACGCAA+AGG | - | chr4.4:2893444-2893463 | None:intergenic | 50.0% | |
| CATTGCTTCCAGTCACTGAC+AGG | + | chr4.4:2893063-2893082 | MS.gene05927:CDS | 50.0% | |
| TGGTAACAACTGGTGCAGCA+AGG | - | chr4.4:2893379-2893398 | None:intergenic | 50.0% | |
| ! | CACAGGGACGAAGTTTTTCG+TGG | + | chr4.4:2893568-2893587 | MS.gene05927:intron | 50.0% |
| CTTAGCTCAGGCAGTGCAGA+AGG | + | chr4.4:2893721-2893740 | MS.gene05927:CDS | 55.0% | |
| GAAGGACCGTGTTGCATTGC+TGG | + | chr4.4:2893739-2893758 | MS.gene05927:CDS | 55.0% | |
| GTACTGTGCAGTCTGTGCTG+TGG | + | chr4.4:2893243-2893262 | MS.gene05927:intron | 55.0% | |
| TGCACAGTACAGTGCCTCCA+AGG | - | chr4.4:2893233-2893252 | None:intergenic | 55.0% | |
| !! | GCTTACGGGTGGCTAGCTTA+TGG | + | chr4.4:2892953-2892972 | MS.gene05927:CDS | 55.0% |
| !! | TCACCTGTTTCTGGTTGCGC+AGG | + | chr4.4:2893006-2893025 | MS.gene05927:CDS | 55.0% |
| GTGCAGTCTGTGCTGTGGCT+GGG | + | chr4.4:2893248-2893267 | MS.gene05927:intron | 60.0% | |
| TGTGCAGTCTGTGCTGTGGC+TGG | + | chr4.4:2893247-2893266 | MS.gene05927:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 2892208 | 2893769 | 2892208 | ID=MS.gene05927 |
| chr4.4 | mRNA | 2892208 | 2893769 | 2892208 | ID=MS.gene05927.t1;Parent=MS.gene05927 |
| chr4.4 | exon | 2892208 | 2892300 | 2892208 | ID=MS.gene05927.t1.exon1;Parent=MS.gene05927.t1 |
| chr4.4 | CDS | 2892208 | 2892300 | 2892208 | ID=cds.MS.gene05927.t1;Parent=MS.gene05927.t1 |
| chr4.4 | exon | 2892916 | 2893084 | 2892916 | ID=MS.gene05927.t1.exon2;Parent=MS.gene05927.t1 |
| chr4.4 | CDS | 2892916 | 2893084 | 2892916 | ID=cds.MS.gene05927.t1;Parent=MS.gene05927.t1 |
| chr4.4 | exon | 2893573 | 2893769 | 2893573 | ID=MS.gene05927.t1.exon3;Parent=MS.gene05927.t1 |
| chr4.4 | CDS | 2893573 | 2893769 | 2893573 | ID=cds.MS.gene05927.t1;Parent=MS.gene05927.t1 |
| Gene Sequence |
| Protein sequence |
