AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059307

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059307.t1 MTR_2g087980 95.070 710 34 1 1 709 1 710 0.0 1389
MS.gene059307.t1 MTR_2g087960 88.194 720 74 4 1 709 1 720 0.0 1294
MS.gene059307.t1 MTR_2g087960 87.676 568 59 4 1 557 1 568 0.0 1008
MS.gene059307.t1 MTR_4g050000 59.078 716 279 7 1 707 1 711 0.0 852
MS.gene059307.t1 MTR_2g087900 58.184 727 282 10 1 709 3 725 0.0 828
MS.gene059307.t1 MTR_2g087950 57.521 718 283 8 1 708 1 706 0.0 805
MS.gene059307.t1 MTR_2g087900 59.792 577 213 7 1 561 3 576 0.0 696
MS.gene059307.t1 MTR_2g087900 58.811 471 174 8 1 455 3 469 0.0 540
MS.gene059307.t1 MTR_2g087900 64.826 344 113 4 1 336 3 346 8.23e-155 452
MS.gene059307.t1 MTR_4g130510 34.749 777 430 17 1 710 228 994 5.69e-133 418
MS.gene059307.t1 MTR_8g086600 34.681 767 407 18 21 710 196 945 2.59e-131 413
MS.gene059307.t1 MTR_2g016630 34.371 771 407 18 21 710 181 933 5.72e-131 412
MS.gene059307.t1 MTR_3g030040 32.709 801 434 18 1 710 248 1034 9.49e-131 414
MS.gene059307.t1 MTR_3g060220 33.379 734 435 14 1 710 1 704 1.64e-129 401
MS.gene059307.t1 MTR_3g107520 33.933 778 414 16 18 709 278 1041 2.94e-129 410
MS.gene059307.t1 MTR_3g060280 33.243 740 420 17 8 710 10 712 3.32e-129 400
MS.gene059307.t1 MTR_3g060220 33.333 735 435 14 1 710 1 705 1.72e-128 398
MS.gene059307.t1 MTR_3g060240 35.573 655 364 15 1 628 1 624 8.76e-128 396
MS.gene059307.t1 MTR_8g063270 33.602 744 417 18 26 710 7 732 1.42e-127 397
MS.gene059307.t1 MTR_1g069605 32.812 768 437 16 8 710 95 848 8.06e-127 399
MS.gene059307.t1 MTR_4g130510 33.292 811 430 18 1 710 228 1028 3.56e-126 401
MS.gene059307.t1 MTR_8g063270 32.859 773 439 19 1 710 182 937 1.30e-125 397
MS.gene059307.t1 MTR_8g037260 32.199 764 424 16 29 710 283 1034 5.47e-124 395
MS.gene059307.t1 MTR_7g106680 31.750 800 439 16 1 710 206 988 1.08e-123 394
MS.gene059307.t1 MTR_1g098550 32.364 791 435 17 8 710 271 1049 1.42e-121 389
MS.gene059307.t1 MTR_1g098550 32.364 791 435 17 8 710 275 1053 1.90e-121 389
MS.gene059307.t1 MTR_4g130510 36.435 634 331 14 1 571 228 852 2.83e-118 375
MS.gene059307.t1 MTR_3g060280 34.768 581 314 11 8 557 10 556 3.90e-111 348
MS.gene059307.t1 MTR_3g060280 34.768 581 314 11 8 557 10 556 3.96e-111 348
MS.gene059307.t1 MTR_5g029190 29.717 848 432 21 8 710 252 1080 8.89e-108 353
MS.gene059307.t1 MTR_1g039445 29.562 822 433 18 18 710 258 1062 2.64e-106 349
MS.gene059307.t1 MTR_4g088225 33.136 676 420 16 45 710 57 710 4.20e-106 339
MS.gene059307.t1 MTR_4g088225 33.136 676 420 16 45 710 57 710 4.37e-106 340
MS.gene059307.t1 MTR_4g077910 29.621 844 438 20 8 710 273 1101 8.92e-106 348
MS.gene059307.t1 MTR_2g037830 31.497 708 437 15 16 706 8 684 4.65e-105 336
MS.gene059307.t1 MTR_1g073740 32.769 650 387 12 4 628 6 630 1.45e-104 336
MS.gene059307.t1 MTR_1g039480 29.398 830 426 18 26 710 276 1090 2.68e-104 344
MS.gene059307.t1 MTR_0011s0020 30.935 695 448 13 20 708 18 686 8.36e-104 332
MS.gene059307.t1 MTR_2g438220 29.197 822 437 21 21 710 175 983 1.31e-102 337
MS.gene059307.t1 MTR_3g005560 30.156 703 455 13 16 706 8 686 3.09e-102 328
MS.gene059307.t1 MTR_7g109200 28.806 854 448 20 2 710 291 1129 5.16e-101 335
MS.gene059307.t1 MTR_3g450800 29.758 662 429 13 54 708 5 637 1.81e-97 314
MS.gene059307.t1 MTR_2g087900 62.241 241 85 3 1 235 3 243 6.30e-96 298
MS.gene059307.t1 MTR_3g005560 32.428 552 341 10 16 557 8 537 6.41e-94 303
MS.gene059307.t1 MTR_2g037830 35.258 485 281 9 16 489 8 470 1.62e-88 288
MS.gene059307.t1 MTR_4g130510 36.400 500 254 12 1 438 228 725 3.35e-88 292
MS.gene059307.t1 MTR_8g092590 41.266 395 203 9 1 368 266 658 7.44e-86 293
MS.gene059307.t1 MTR_8g092590 30.337 356 214 8 372 710 714 1052 4.44e-40 159
MS.gene059307.t1 MTR_7g116230 31.475 610 301 17 8 524 129 714 1.85e-84 285
MS.gene059307.t1 MTR_3g060280 37.340 391 207 7 8 367 10 393 3.87e-84 273
MS.gene059307.t1 MTR_2g035780 41.026 390 201 9 8 370 206 593 4.95e-82 281
MS.gene059307.t1 MTR_2g035780 29.970 337 215 7 381 710 678 1000 4.16e-38 152
MS.gene059307.t1 MTR_3g007770 38.916 406 218 10 1 378 215 618 5.55e-80 276
MS.gene059307.t1 MTR_3g007770 31.343 335 215 7 381 709 679 1004 3.11e-39 156
MS.gene059307.t1 MTR_3g005560 34.023 435 256 9 16 440 8 421 9.34e-75 248
MS.gene059307.t1 MTR_3g091270 31.944 432 197 12 87 462 106 496 8.15e-54 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059307.t1 AT2G32530 45.144 731 360 9 8 709 10 728 0.0 624
MS.gene059307.t1 AT2G32540 45.691 731 356 11 8 709 10 728 0.0 612
MS.gene059307.t1 AT4G15320 44.218 735 363 13 7 707 9 730 0.0 598
MS.gene059307.t1 AT4G15320 44.218 735 363 13 7 707 9 730 0.0 597
MS.gene059307.t1 AT4G15320 43.333 750 363 14 7 707 9 745 0.0 590
MS.gene059307.t1 AT2G32620 44.993 729 366 9 8 709 10 730 0.0 582
MS.gene059307.t1 AT2G32610 42.701 733 379 13 8 709 10 732 0.0 582
MS.gene059307.t1 AT4G15290 42.740 730 377 13 8 707 10 728 0.0 559
MS.gene059307.t1 AT4G15320 42.547 691 306 12 7 633 9 672 0.0 535
MS.gene059307.t1 AT2G32620 46.734 597 295 7 8 589 10 598 8.64e-178 521
MS.gene059307.t1 AT2G32620 44.081 642 326 8 93 709 1 634 6.02e-175 516
MS.gene059307.t1 AT4G15320 40.191 734 305 13 7 633 9 715 1.41e-173 518
MS.gene059307.t1 AT2G32620 48.566 523 243 7 8 513 10 523 5.64e-160 473
MS.gene059307.t1 AT5G17420 34.699 781 426 18 1 710 215 982 4.92e-135 424
MS.gene059307.t1 AT4G18780 34.788 779 406 15 16 710 179 939 2.03e-134 421
MS.gene059307.t1 AT4G32410 34.000 800 423 19 1 710 252 1036 2.72e-133 420
MS.gene059307.t1 AT5G09870 33.250 797 433 16 1 710 241 1025 6.64e-133 419
MS.gene059307.t1 AT5G05170 33.667 799 428 18 1 710 236 1021 1.44e-132 418
MS.gene059307.t1 AT2G25540 34.043 799 424 20 1 710 232 1016 1.23e-130 413
MS.gene059307.t1 AT1G55850 35.471 733 395 20 8 710 18 702 2.58e-130 403
MS.gene059307.t1 AT5G64740 32.877 803 431 16 1 710 253 1040 2.53e-129 410
MS.gene059307.t1 AT2G21770 33.543 796 432 20 1 710 259 1043 2.48e-127 405
MS.gene059307.t1 AT4G24000 32.565 694 422 12 27 709 34 692 6.38e-112 355
MS.gene059307.t1 AT4G23990 32.042 671 403 11 57 709 86 721 2.92e-111 354
MS.gene059307.t1 AT4G24010 33.283 658 382 14 57 696 67 685 6.38e-111 353
MS.gene059307.t1 AT1G32180 29.684 822 437 17 25 709 123 940 7.41e-106 345
MS.gene059307.t1 AT1G32180 29.684 822 437 17 25 709 123 940 7.41e-106 345
MS.gene059307.t1 AT4G38190 28.801 809 436 17 26 710 273 1065 1.32e-104 344
MS.gene059307.t1 AT2G33100 29.335 842 445 20 8 710 167 997 3.56e-104 342
MS.gene059307.t1 AT2G33100 29.335 842 445 20 8 710 167 997 3.56e-104 342
MS.gene059307.t1 AT1G02730 29.002 862 443 20 2 710 296 1141 2.97e-102 339
MS.gene059307.t1 AT3G03050 29.097 842 443 17 8 710 278 1104 1.47e-100 334
MS.gene059307.t1 AT5G16910 29.462 818 432 17 23 710 302 1104 4.19e-99 330
MS.gene059307.t1 AT4G39350 41.162 396 202 10 1 368 254 646 3.78e-85 291
MS.gene059307.t1 AT4G39350 29.573 328 218 5 386 710 722 1039 4.23e-35 143
MS.gene059307.t1 AT5G44030 40.256 390 204 9 8 370 199 586 2.07e-81 280
MS.gene059307.t1 AT5G44030 30.368 326 216 4 388 710 688 1005 1.17e-37 151
MS.gene059307.t1 AT5G44030 40.256 390 204 9 8 370 228 615 2.59e-81 280
MS.gene059307.t1 AT5G44030 30.368 326 216 4 388 710 717 1034 1.03e-37 152
MS.gene059307.t1 AT4G24000 32.210 534 311 12 192 709 2 500 2.47e-74 250
MS.gene059307.t1 AT5G44030 43.813 299 153 5 86 370 10 307 6.52e-73 252
MS.gene059307.t1 AT5G44030 30.368 326 216 4 388 710 409 726 3.58e-38 152
MS.gene059307.t1 AT4G23990 30.499 541 318 12 192 709 2 507 7.15e-71 241

Find 147 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGGTAAAATCCAATTT+AGG 0.109508 2.2:+11263785 MS.gene059307:CDS
AGCACTTGTTATTAAGATTT+TGG 0.119897 2.2:+11264666 MS.gene059307:CDS
AACCCAAAACTTAGCAAATT+TGG 0.129090 2.2:-11261632 None:intergenic
CTACAAGAGTATAAAGTAAA+AGG 0.191998 2.2:+11264397 MS.gene059307:CDS
CTTCCAAATTTGCTAAGTTT+TGG 0.203327 2.2:+11261629 MS.gene059307:CDS
GATTGCTTCCTCTATAAAAT+TGG 0.205458 2.2:-11263387 None:intergenic
TTGTAATTGTGTTCATAATT+TGG 0.215230 2.2:-11262366 None:intergenic
TCAACAAGTGTATGGTTTAT+AGG 0.235726 2.2:+11264469 MS.gene059307:CDS
CACAGAAGATATGCTATTTA+CGG 0.237161 2.2:+11262868 MS.gene059307:CDS
CCTGTTATGGGTACCCTCTT+TGG 0.238815 2.2:+11263769 MS.gene059307:CDS
CTTCAGATGGGCTGGTTATA+TGG 0.244829 2.2:+11263565 MS.gene059307:intron
AACCATTCTTGTTTGAATTC+TGG 0.245579 2.2:-11261733 None:intergenic
AAGACAAGCCAGCCCTAAAT+TGG 0.251195 2.2:-11263798 None:intergenic
AGTCCAACTTCTGGTGTTAT+TGG 0.253634 2.2:+11264559 MS.gene059307:CDS
TTGGAATGAAGCTGAATTTC+GGG 0.257400 2.2:-11261952 None:intergenic
CTTATTGTTGGCTTACCAAT+TGG 0.259289 2.2:+11263818 MS.gene059307:CDS
CCCGAGCCAACCGCGTTTAC+AGG 0.262333 2.2:+11263658 MS.gene059307:CDS
TAGCAAGCATTCTCCTGTTA+TGG 0.266654 2.2:+11263756 MS.gene059307:CDS
AGTGCCATGCTTAAGCTCTT+AGG 0.270075 2.2:+11264499 MS.gene059307:CDS
CTTTGGTAAAATCCAATTTA+GGG 0.273325 2.2:+11263786 MS.gene059307:CDS
TTGCTTTCTCATGTGAATCT+TGG 0.274164 2.2:+11260792 MS.gene059307:CDS
TATCCTAATCAAATACAATA+TGG 0.281501 2.2:+11262895 MS.gene059307:CDS
AGCTTCCAAAAGACCATAAA+AGG 0.292008 2.2:-11261608 None:intergenic
TTTCCATATTGTATTTGATT+AGG 0.299333 2.2:-11262898 None:intergenic
CTGCAAATTCTCCCTCAAAT+TGG 0.301372 2.2:-11261971 None:intergenic
TATGCGTTGACAGGTTATAT+TGG 0.311069 2.2:+11262290 MS.gene059307:intron
TTCTGGTGAATCATCACTTT+TGG 0.313648 2.2:-11261716 None:intergenic
TGTATCTAGTAGTTTGTTAC+TGG 0.314730 2.2:+11264749 MS.gene059307:CDS
GCTTGTTATGTTTCTGATGA+TGG 0.315570 2.2:+11261571 MS.gene059307:CDS
GGAATAAAAGACGACCCTTT+TGG 0.316228 2.2:+11262669 MS.gene059307:CDS
AGCAAGCATTCTCCTGTTAT+GGG 0.321276 2.2:+11263757 MS.gene059307:CDS
ACATGCTATTTGCATATTGA+TGG 0.332778 2.2:+11262590 MS.gene059307:CDS
CAGTCTACGTTCAACATAAA+AGG 0.336254 2.2:-11262534 None:intergenic
GAATCATCCAAGTATAATTA+AGG 0.336801 2.2:+11262016 MS.gene059307:CDS
AATAGCATGTTGCATAATCT+TGG 0.337211 2.2:-11262577 None:intergenic
ATTGGAATGAAGCTGAATTT+CGG 0.338929 2.2:-11261953 None:intergenic
TTATTGTTGGCTTACCAATT+GGG 0.340053 2.2:+11263819 MS.gene059307:CDS
GGGCTGGCTTGTCTTATTGT+TGG 0.343431 2.2:+11263806 MS.gene059307:CDS
CAAATACAATATGGAAATAA+AGG 0.347147 2.2:+11262904 MS.gene059307:CDS
GCCTGTAAACGCGGTTGGCT+CGG 0.353153 2.2:-11263659 None:intergenic
CATATTCACAATCAGATACT+TGG 0.373087 2.2:-11263409 None:intergenic
TCAGCAACACAAGTTATAGA+AGG 0.377465 2.2:+11263332 MS.gene059307:CDS
ACTTGAGAGAAATATCTAAA+AGG 0.378307 2.2:-11261691 None:intergenic
TACCAATTGGGGTCTGCGTT+CGG 0.380901 2.2:+11263831 MS.gene059307:CDS
TTCTGAAAGGGTTGTTTGCT+AGG 0.385850 2.2:+11264776 MS.gene059307:CDS
AGTGGGAAAGAGTGTGGAAT+AGG 0.391608 2.2:+11264709 MS.gene059307:CDS
TTGTTGTATTAGTACATTCT+CGG 0.396375 2.2:-11263282 None:intergenic
ACTGTCTTATTCCAGGGAGC+TGG 0.397150 2.2:+11264328 MS.gene059307:intron
CCAAAGAGGGTACCCATAAC+AGG 0.401351 2.2:-11263769 None:intergenic
AATGTACTAATACAACAATT+TGG 0.401655 2.2:+11263287 MS.gene059307:CDS
TTTATTACCTTAATTATACT+TGG 0.407006 2.2:-11262023 None:intergenic
TAGCACTTGTTGGGGTAAAC+AGG 0.409962 2.2:+11263436 MS.gene059307:CDS
TCAATGGGTGGCTGCATTTG+AGG 0.415447 2.2:+11262695 MS.gene059307:CDS
TATTCCAGGGAGCTGGGCTA+TGG 0.415813 2.2:+11264335 MS.gene059307:intron
CCTGTAAACGCGGTTGGCTC+GGG 0.421920 2.2:-11263658 None:intergenic
ATATGAATATAGCACTTGTT+GGG 0.423823 2.2:+11263427 MS.gene059307:CDS
TGAATATGCAAAGGAAAGGT+TGG 0.428367 2.2:+11263617 MS.gene059307:CDS
GACCGAACGCAGACCCCAAT+TGG 0.428493 2.2:-11263833 None:intergenic
CAACAAAAGAGATGGTCTTC+AGG 0.429438 2.2:+11263715 MS.gene059307:CDS
GTGCCATGCTTAAGCTCTTA+GGG 0.430274 2.2:+11264500 MS.gene059307:CDS
GCAAACAACCCTTTCAGAAA+TGG 0.433149 2.2:-11264772 None:intergenic
TTCAGCTTCATTCCAATTTG+AGG 0.436875 2.2:+11261959 MS.gene059307:CDS
TACCGTTGAAGGAGACGGTT+TGG 0.441157 2.2:-11260878 None:intergenic
GGACTTCAAGGACCATACTA+TGG 0.444865 2.2:+11262829 MS.gene059307:CDS
GTTACTGGCCATTTCTGAAA+GGG 0.448294 2.2:+11264764 MS.gene059307:CDS
GGGAGTGATTATTCTACTAA+TGG 0.449793 2.2:+11263353 MS.gene059307:CDS
TTTATATTACATACCGTTGA+AGG 0.450188 2.2:-11260889 None:intergenic
CACCAGAATTCAAACAAGAA+TGG 0.451883 2.2:+11261731 MS.gene059307:CDS
GTGTTTGATCCTCCATAGTA+TGG 0.454323 2.2:-11262841 None:intergenic
CAATAAGATCAACAAGTGTA+TGG 0.456139 2.2:+11264461 MS.gene059307:CDS
TCAGCTTCATTCCAATTTGA+GGG 0.456850 2.2:+11261960 MS.gene059307:CDS
GTTGTGCCAACCACAAAAGC+TGG 0.457175 2.2:-11264622 None:intergenic
ATAGCATGTTGCATAATCTT+GGG 0.464154 2.2:-11262576 None:intergenic
TTATTCTTCTCAATACCAAA+TGG 0.468145 2.2:+11260831 MS.gene059307:CDS
CATTGTAGTAAGTAATCCTC+CGG 0.471086 2.2:-11263689 None:intergenic
GCCACCCATTGATTTCCAAA+AGG 0.473772 2.2:-11262684 None:intergenic
ACAAGAATGGTTACAAATGA+AGG 0.473858 2.2:+11261744 MS.gene059307:CDS
TGTCAACTGCACAAGCAAAA+TGG 0.478341 2.2:-11264644 None:intergenic
TATTGAAACTTCAGATGGGC+TGG 0.478900 2.2:+11263557 MS.gene059307:intron
GGTAAAATCCAATTTAGGGC+TGG 0.479956 2.2:+11263790 MS.gene059307:CDS
GTTAACAGCAGGACTCCATT+TGG 0.481702 2.2:-11260846 None:intergenic
ATGATTGGTGGTTCTAGAAC+AGG 0.483104 2.2:-11261493 None:intergenic
CCACCCATTGATTTCCAAAA+GGG 0.486982 2.2:-11262683 None:intergenic
TCTGAAAGGGTTGTTTGCTA+GGG 0.487780 2.2:+11264777 MS.gene059307:CDS
CAAAAGGAAAGTCCAACTTC+TGG 0.490074 2.2:+11264550 MS.gene059307:CDS
AATTTGCAGACAAGAGTCTC+TGG 0.491545 2.2:+11262495 MS.gene059307:intron
TAACAAGCTAGCTTATTAGC+TGG 0.493726 2.2:-11261556 None:intergenic
TACCAACACATTCATAGCTC+CGG 0.494183 2.2:-11262391 None:intergenic
TACAAGAGTATAAAGTAAAA+GGG 0.495125 2.2:+11264398 MS.gene059307:CDS
ATATTGGATGGTCATTCTGA+TGG 0.497574 2.2:+11262306 MS.gene059307:CDS
AATATGAATATAGCACTTGT+TGG 0.497803 2.2:+11263426 MS.gene059307:CDS
AGTCTTTGAAGTAACACAAA+AGG 0.498554 2.2:+11264534 MS.gene059307:CDS
GGTAACAAAGAAGAGAGAAT+AGG 0.498755 2.2:-11260736 None:intergenic
ATATCAGAAGATGTACCAAC+TGG 0.500267 2.2:+11263592 MS.gene059307:CDS
GCGTTTACAGGCTGTGCTCC+CGG 0.502923 2.2:+11263670 MS.gene059307:CDS
CGTTGACAGGTTATATTGGA+TGG 0.505316 2.2:+11262294 MS.gene059307:intron
CTGTCTTATTCCAGGGAGCT+GGG 0.505938 2.2:+11264329 MS.gene059307:intron
TATTGTTGGCTTACCAATTG+GGG 0.508021 2.2:+11263820 MS.gene059307:CDS
ATATTATACTTCTTACAGAA+AGG 0.520036 2.2:-11261655 None:intergenic
TTGTGAAGGTGTTGTTGATA+CGG 0.520835 2.2:-11260757 None:intergenic
TGATTATGCAATATGCAACT+AGG 0.523140 2.2:-11263876 None:intergenic
CGTAAATAGCATATCTTCTG+TGG 0.523357 2.2:-11262867 None:intergenic
AATCTTGGTTCACATACACT+TGG 0.525534 2.2:+11260807 MS.gene059307:CDS
GCTTCCTCTATAAAATTGGA+AGG 0.528133 2.2:-11263383 None:intergenic
ACAACAATTTGGAAGTTCAA+AGG 0.535086 2.2:+11263298 MS.gene059307:CDS
CTTTGCATATTCAATCCAGT+TGG 0.535136 2.2:-11263607 None:intergenic
GATTATGCAATATGCAACTA+GGG 0.540386 2.2:-11263875 None:intergenic
GTTCAACATAAAAGGAGCAT+TGG 0.542027 2.2:-11262526 None:intergenic
ACAACACCTTCACAATTCCA+TGG 0.549695 2.2:+11260765 MS.gene059307:CDS
TGTTACTGGCCATTTCTGAA+AGG 0.550252 2.2:+11264763 MS.gene059307:CDS
TCTCCAATAACACCAGAAGT+TGG 0.551519 2.2:-11264562 None:intergenic
TGCAATTAGAAGCTCATAGT+GGG 0.552228 2.2:+11264692 MS.gene059307:CDS
ACAGTGTTCAATGTGATGAT+TGG 0.552842 2.2:-11261508 None:intergenic
TATGAACACAATTACAAAGC+CGG 0.562840 2.2:+11262372 MS.gene059307:CDS
AACAAAGAAGAGAGAATAGG+AGG 0.565555 2.2:-11260733 None:intergenic
TGCTAAAGAAGACAACTGTA+AGG 0.565575 2.2:-11263738 None:intergenic
GTGCAATTAGAAGCTCATAG+TGG 0.566989 2.2:+11264691 MS.gene059307:CDS
ATCCAAACCGTCTCCTTCAA+CGG 0.575866 2.2:+11260876 MS.gene059307:CDS
CTGAAAGGGTTGTTTGCTAG+GGG 0.575876 2.2:+11264778 MS.gene059307:CDS
CATGAGAAAGCAAGAAACCA+TGG 0.578989 2.2:-11260782 None:intergenic
TTGTGCCAACCACAAAAGCT+GGG 0.580530 2.2:-11264621 None:intergenic
ATATTGATGGATTCTAAGAA+CGG 0.581409 2.2:+11262603 MS.gene059307:CDS
GTGTTCAATGTGATGATTGG+TGG 0.582750 2.2:-11261505 None:intergenic
TTGGTGGAACAATCAGAGAA+TGG 0.583369 2.2:+11264435 MS.gene059307:CDS
CAGCAACACAAGTTATAGAA+GGG 0.583862 2.2:+11263333 MS.gene059307:CDS
GCACAGCCTGTAAACGCGGT+TGG 0.586028 2.2:-11263664 None:intergenic
AATAGCATATCTTCTGTGGA+AGG 0.597992 2.2:-11262863 None:intergenic
TTACATACCGTTGAAGGAGA+CGG 0.598803 2.2:-11260883 None:intergenic
ATTGGAGATGATGCAAATGC+TGG 0.605052 2.2:+11264577 MS.gene059307:CDS
AGTACAGGAGCTTCCACGTG+TGG 0.608658 2.2:+11261447 MS.gene059307:CDS
CAATGATCCAACAAAAGAGA+TGG 0.612047 2.2:+11263707 MS.gene059307:CDS
CTCGATATGTAGATTAAGTG+AGG 0.612917 2.2:-11262333 None:intergenic
AGCCGGAGCTATGAATGTGT+TGG 0.613715 2.2:+11262389 MS.gene059307:CDS
CAACTGGATTGAATATGCAA+AGG 0.619420 2.2:+11263608 MS.gene059307:CDS
AAGAAACCATGGAATTGTGA+AGG 0.621466 2.2:-11260771 None:intergenic
TATGAATATAGCACTTGTTG+GGG 0.627581 2.2:+11263428 MS.gene059307:CDS
ATTGTAGTAAGTAATCCTCC+GGG 0.635850 2.2:-11263688 None:intergenic
GGATTGAATATGCAAAGGAA+AGG 0.635952 2.2:+11263613 MS.gene059307:CDS
GCTCATAGTGGGAAAGAGTG+TGG 0.637563 2.2:+11264703 MS.gene059307:CDS
TATCCCTAAGAGCTTAAGCA+TGG 0.651527 2.2:-11264503 None:intergenic
GGGAGCACAGCCTGTAAACG+CGG 0.667882 2.2:-11263668 None:intergenic
CTTCAAGGACCATACTATGG+AGG 0.676748 2.2:+11262832 MS.gene059307:CDS
TTTACAGGCTGTGCTCCCGG+AGG 0.680678 2.2:+11263673 MS.gene059307:CDS
TGTGCCAACCACAAAAGCTG+GGG 0.694857 2.2:-11264620 None:intergenic
GAGATGATGCAAATGCTGGT+AGG 0.699231 2.2:+11264581 MS.gene059307:CDS
GTCACAAACAAATCCACACG+TGG 0.721171 2.2:-11261460 None:intergenic
GTTGAACGTAGACTGTGACA+TGG 0.762585 2.2:+11262542 MS.gene059307:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTACCTTAATTATACT+TGG - chr2.2:11262026-11262045 None:intergenic 15.0%
!!! AAATTTAGACAACTTTTTAA+GGG - chr2.2:11261350-11261369 None:intergenic 15.0%
!!! TTTTGGAAATAATATAACAA+CGG + chr2.2:11264178-11264197 MS.gene059307:intron 15.0%
!! AAACTAATTAGCAAGATAAA+TGG - chr2.2:11261384-11261403 None:intergenic 20.0%
!! AATGTACTAATACAACAATT+TGG + chr2.2:11263287-11263306 MS.gene059307:CDS 20.0%
!! AGTTGAAAACTTGAAAATTA+TGG + chr2.2:11261263-11261282 MS.gene059307:intron 20.0%
!! ATATTATACTTCTTACAGAA+AGG - chr2.2:11261658-11261677 None:intergenic 20.0%
!! ATTTGTTCAATTATAGAACA+AGG - chr2.2:11262093-11262112 None:intergenic 20.0%
!! CAAAAACTGTTAAAATTAGT+CGG - chr2.2:11264163-11264182 None:intergenic 20.0%
!! CAAATACAATATGGAAATAA+AGG + chr2.2:11262904-11262923 MS.gene059307:CDS 20.0%
!! CATTTAATCATAAAATCAGT+TGG + chr2.2:11264016-11264035 MS.gene059307:intron 20.0%
!! CTTAAAAAGTTGTCTAAATT+TGG + chr2.2:11261349-11261368 MS.gene059307:intron 20.0%
!! TACAAACACAAATTTATAAG+TGG - chr2.2:11262762-11262781 None:intergenic 20.0%
!! TATCCTAATCAAATACAATA+TGG + chr2.2:11262895-11262914 MS.gene059307:CDS 20.0%
!! TATTCTCAAATACAGAAAAA+AGG + chr2.2:11261994-11262013 MS.gene059307:CDS 20.0%
!! TTGTAATTGTGTTCATAATT+TGG - chr2.2:11262369-11262388 None:intergenic 20.0%
!! TTTCCATATTGTATTTGATT+AGG - chr2.2:11262901-11262920 None:intergenic 20.0%
!!! AATTTAGACAACTTTTTAAG+GGG - chr2.2:11261349-11261368 None:intergenic 20.0%
!!! ATAATTGAACAAATTTTAGC+AGG + chr2.2:11262099-11262118 MS.gene059307:intron 20.0%
!!! CAAATTTAGACAACTTTTTA+AGG - chr2.2:11261351-11261370 None:intergenic 20.0%
!!! TACAAGAGTATAAAGTAAAA+GGG + chr2.2:11264398-11264417 MS.gene059307:CDS 20.0%
!!! TTATTCCATCGTAAAATTTT+TGG - chr2.2:11262656-11262675 None:intergenic 20.0%
!!! TTGTGTAATTTTATAGTCTA+AGG - chr2.2:11263504-11263523 None:intergenic 20.0%
! AATATGAATATAGCACTTGT+TGG + chr2.2:11263426-11263445 MS.gene059307:CDS 25.0%
! ACTTGAGAGAAATATCTAAA+AGG - chr2.2:11261694-11261713 None:intergenic 25.0%
! AGAAATACTTGGAAGAATTT+AGG - chr2.2:11262153-11262172 None:intergenic 25.0%
! ATAAGTATCTAAATCTATGG+TGG + chr2.2:11261812-11261831 MS.gene059307:intron 25.0%
! ATATGAATATAGCACTTGTT+GGG + chr2.2:11263427-11263446 MS.gene059307:CDS 25.0%
! CTCATTCTTATCTTTCTTAA+CGG + chr2.2:11262471-11262490 MS.gene059307:intron 25.0%
! CTTTGGTAAAATCCAATTTA+GGG + chr2.2:11263786-11263805 MS.gene059307:CDS 25.0%
! GAAATAATATAACAACGGTT+AGG + chr2.2:11264183-11264202 MS.gene059307:intron 25.0%
! GAATCATCCAAGTATAATTA+AGG + chr2.2:11262016-11262035 MS.gene059307:CDS 25.0%
! GAGATAAGTATCTAAATCTA+TGG + chr2.2:11261809-11261828 MS.gene059307:intron 25.0%
! GGTATATCTATTATCATCTA+CGG - chr2.2:11261164-11261183 None:intergenic 25.0%
! GTTAATGCAGATTAAAGATA+AGG - chr2.2:11261327-11261346 None:intergenic 25.0%
! TAAATTCTTCCAAGTATTTC+TGG + chr2.2:11262152-11262171 MS.gene059307:intron 25.0%
! TATCTATTGAAACTTCAGAT+GGG + chr2.2:11263553-11263572 MS.gene059307:intron 25.0%
! TATGCTTGAGTAATTATATG+AGG + chr2.2:11263170-11263189 MS.gene059307:intron 25.0%
! TCATTCTTATCTTTCTTAAC+GGG + chr2.2:11262472-11262491 MS.gene059307:intron 25.0%
! TCTTTGGTAAAATCCAATTT+AGG + chr2.2:11263785-11263804 MS.gene059307:CDS 25.0%
! TTATCTATTGAAACTTCAGA+TGG + chr2.2:11263552-11263571 MS.gene059307:intron 25.0%
! TTATTCTTCTCAATACCAAA+TGG + chr2.2:11260831-11260850 MS.gene059307:CDS 25.0%
! TTGTTGTATTAGTACATTCT+CGG - chr2.2:11263285-11263304 None:intergenic 25.0%
! TTTATATTACATACCGTTGA+AGG - chr2.2:11260892-11260911 None:intergenic 25.0%
!! AAATTGGATTTTACCAAAGA+GGG - chr2.2:11263785-11263804 None:intergenic 25.0%
!! AAGGGTTTTCATTAAGATAT+TGG + chr2.2:11264416-11264435 MS.gene059307:CDS 25.0%
!! ATTGAGCTTAAGTTGTTAAT+TGG - chr2.2:11263132-11263151 None:intergenic 25.0%
!! CTACAAGAGTATAAAGTAAA+AGG + chr2.2:11264397-11264416 MS.gene059307:CDS 25.0%
!! CTCAATGTTTAATTTAACCT+TGG - chr2.2:11263953-11263972 None:intergenic 25.0%
!! TAAATTGGATTTTACCAAAG+AGG - chr2.2:11263786-11263805 None:intergenic 25.0%
!! TATTTTATCGGAATGTGATA+TGG + chr2.2:11262970-11262989 MS.gene059307:intron 25.0%
!! TGTTTCCAAAAATTTTACGA+TGG + chr2.2:11262648-11262667 MS.gene059307:CDS 25.0%
!! TTTTACCTTTTATGGTCTTT+TGG + chr2.2:11261603-11261622 MS.gene059307:CDS 25.0%
!!! ACTTGTTATTAAGATTTTGG+GGG + chr2.2:11264669-11264688 MS.gene059307:CDS 25.0%
!!! AGCACTTGTTATTAAGATTT+TGG + chr2.2:11264666-11264685 MS.gene059307:CDS 25.0%
!!! ATATTGATGGATTCTAAGAA+CGG + chr2.2:11262603-11262622 MS.gene059307:CDS 25.0%
!!! ATCCATATTTTTTCATACAG+AGG - chr2.2:11260671-11260690 None:intergenic 25.0%
!!! CACTTGTTATTAAGATTTTG+GGG + chr2.2:11264668-11264687 MS.gene059307:CDS 25.0%
!!! CGACTAATTTTAACAGTTTT+TGG + chr2.2:11264161-11264180 MS.gene059307:intron 25.0%
!!! GCACTTGTTATTAAGATTTT+GGG + chr2.2:11264667-11264686 MS.gene059307:CDS 25.0%
!!! TCCATATTTTTTCATACAGA+GGG - chr2.2:11260670-11260689 None:intergenic 25.0%
!!! TGTTCTGTTTTTACCTTTTA+TGG + chr2.2:11261595-11261614 MS.gene059307:CDS 25.0%
!!! TTCCAAATTTGCTAAGTTTT+GGG + chr2.2:11261630-11261649 MS.gene059307:CDS 25.0%
!!! TTGTTAATTGGTTCTAGTTA+AGG - chr2.2:11263120-11263139 None:intergenic 25.0%
AAACTCATTAACCACCAATT+TGG - chr2.2:11261299-11261318 None:intergenic 30.0%
AACCCAAAACTTAGCAAATT+TGG - chr2.2:11261635-11261654 None:intergenic 30.0%
AATAGCATGTTGCATAATCT+TGG - chr2.2:11262580-11262599 None:intergenic 30.0%
ACAACAATTTGGAAGTTCAA+AGG + chr2.2:11263298-11263317 MS.gene059307:CDS 30.0%
ACAAGAATGGTTACAAATGA+AGG + chr2.2:11261744-11261763 MS.gene059307:CDS 30.0%
AGAAAGATAAGAATGAGTCT+CGG - chr2.2:11262468-11262487 None:intergenic 30.0%
ATAGCATGTTGCATAATCTT+GGG - chr2.2:11262579-11262598 None:intergenic 30.0%
ATTGGAATGAAGCTGAATTT+CGG - chr2.2:11261956-11261975 None:intergenic 30.0%
CAAGTAACAAATTTGTAGGA+TGG + chr2.2:11261848-11261867 MS.gene059307:intron 30.0%
CAATAAGATCAACAAGTGTA+TGG + chr2.2:11264461-11264480 MS.gene059307:CDS 30.0%
CATATTCACAATCAGATACT+TGG - chr2.2:11263412-11263431 None:intergenic 30.0%
GACTTGAAAATGGTAGATAA+GGG - chr2.2:11264817-11264836 None:intergenic 30.0%
GATTATGCAATATGCAACTA+GGG - chr2.2:11263878-11263897 None:intergenic 30.0%
GATTGCTTCCTCTATAAAAT+TGG - chr2.2:11263390-11263409 None:intergenic 30.0%
TAGATCACAAAAAGAGTCAA+AGG - chr2.2:11264364-11264383 None:intergenic 30.0%
TATGAACACAATTACAAAGC+CGG + chr2.2:11262372-11262391 MS.gene059307:CDS 30.0%
TCAACAAGTGTATGGTTTAT+AGG + chr2.2:11264469-11264488 MS.gene059307:CDS 30.0%
TCCCTCTGTATGAAAAAATA+TGG + chr2.2:11260666-11260685 MS.gene059307:CDS 30.0%
TGACAATCTTAGCCAAAAAA+TGG + chr2.2:11261920-11261939 MS.gene059307:CDS 30.0%
TGACTTGAAAATGGTAGATA+AGG - chr2.2:11264818-11264837 None:intergenic 30.0%
TGATTATGCAATATGCAACT+AGG - chr2.2:11263879-11263898 None:intergenic 30.0%
TGCACAAGTAACAAATTTGT+AGG + chr2.2:11261844-11261863 MS.gene059307:intron 30.0%
TGTGACTCAATCAATAGTTA+TGG + chr2.2:11260973-11260992 MS.gene059307:intron 30.0%
TGTTAAAATTAGTCGGAGAT+TGG - chr2.2:11264156-11264175 None:intergenic 30.0%
TTCGCAGAGCTAAATAATAT+TGG - chr2.2:11262427-11262446 None:intergenic 30.0%
! AACCATTCTTGTTTGAATTC+TGG - chr2.2:11261736-11261755 None:intergenic 30.0%
! ACATGCTATTTGCATATTGA+TGG + chr2.2:11262590-11262609 MS.gene059307:CDS 30.0%
! CACAGAAGATATGCTATTTA+CGG + chr2.2:11262868-11262887 MS.gene059307:CDS 30.0%
! GGTTTTCATTAAGATATTGG+TGG + chr2.2:11264419-11264438 MS.gene059307:CDS 30.0%
! TAGTCCTTCCAATTTTATAG+AGG + chr2.2:11263379-11263398 MS.gene059307:CDS 30.0%
! TATGAATATAGCACTTGTTG+GGG + chr2.2:11263428-11263447 MS.gene059307:CDS 30.0%
! TTTTTATCCTCATAGAGTAC+AGG + chr2.2:11261432-11261451 MS.gene059307:intron 30.0%
!! AAAAAGTGCTGACTTGAAAA+TGG - chr2.2:11264827-11264846 None:intergenic 30.0%
!! AGTCTTTGAAGTAACACAAA+AGG + chr2.2:11264534-11264553 MS.gene059307:CDS 30.0%
!! CGCTATTTTGTATCTTTACT+AGG + chr2.2:11263251-11263270 MS.gene059307:intron 30.0%
!! TGTATCTAGTAGTTTGTTAC+TGG + chr2.2:11264749-11264768 MS.gene059307:CDS 30.0%
!! TTATTGTTGGCTTACCAATT+GGG + chr2.2:11263819-11263838 MS.gene059307:CDS 30.0%
!!! CGAGTTTTTTAGTATCATAC+AGG - chr2.2:11263233-11263252 None:intergenic 30.0%
!!! CTTCCAAATTTGCTAAGTTT+TGG + chr2.2:11261629-11261648 MS.gene059307:CDS 30.0%
!!! GTTTTTTGCAGTACATGATA+GGG + chr2.2:11262797-11262816 MS.gene059307:intron 30.0%
!!! TGTTTTTTGCAGTACATGAT+AGG + chr2.2:11262796-11262815 MS.gene059307:intron 30.0%
!!! TTTTTTGCAGTACATGATAG+GGG + chr2.2:11262798-11262817 MS.gene059307:intron 30.0%
AACAAAGAAGAGAGAATAGG+AGG - chr2.2:11260736-11260755 None:intergenic 35.0%
AACTCATGTGTGAGTCAATT+AGG - chr2.2:11262245-11262264 None:intergenic 35.0%
AAGAAACCATGGAATTGTGA+AGG - chr2.2:11260774-11260793 None:intergenic 35.0%
AATAGCATATCTTCTGTGGA+AGG - chr2.2:11262866-11262885 None:intergenic 35.0%
AATCTTGGTTCACATACACT+TGG + chr2.2:11260807-11260826 MS.gene059307:CDS 35.0%
ACACAAAGACGAATCATCAA+TGG - chr2.2:11261882-11261901 None:intergenic 35.0%
ACAGTGTTCAATGTGATGAT+TGG - chr2.2:11261511-11261530 None:intergenic 35.0%
AGCTTCCAAAAGACCATAAA+AGG - chr2.2:11261611-11261630 None:intergenic 35.0%
ATATCAGAAGATGTACCAAC+TGG + chr2.2:11263592-11263611 MS.gene059307:CDS 35.0%
ATATTGGATGGTCATTCTGA+TGG + chr2.2:11262306-11262325 MS.gene059307:CDS 35.0%
ATGCAAGAACCAGAAATACT+TGG - chr2.2:11262164-11262183 None:intergenic 35.0%
ATGGTTAACCATGTCCAAAT+TGG + chr2.2:11261282-11261301 MS.gene059307:intron 35.0%
ATGTCTCAGTTTATATGTCG+CGG + chr2.2:11261025-11261044 MS.gene059307:intron 35.0%
ATTAACCACCAATTTGGACA+TGG - chr2.2:11261293-11261312 None:intergenic 35.0%
ATTAGTTGCAACTTGCAAGT+TGG - chr2.2:11264128-11264147 None:intergenic 35.0%
ATTGTAGTAAGTAATCCTCC+GGG - chr2.2:11263691-11263710 None:intergenic 35.0%
CAACTGGATTGAATATGCAA+AGG + chr2.2:11263608-11263627 MS.gene059307:CDS 35.0%
CAATGATCCAACAAAAGAGA+TGG + chr2.2:11263707-11263726 MS.gene059307:CDS 35.0%
CACCAGAATTCAAACAAGAA+TGG + chr2.2:11261731-11261750 MS.gene059307:CDS 35.0%
CAGCAACACAAGTTATAGAA+GGG + chr2.2:11263333-11263352 MS.gene059307:CDS 35.0%
CAGTCTACGTTCAACATAAA+AGG - chr2.2:11262537-11262556 None:intergenic 35.0%
CATTGTAGTAAGTAATCCTC+CGG - chr2.2:11263692-11263711 None:intergenic 35.0%
CGTAAATAGCATATCTTCTG+TGG - chr2.2:11262870-11262889 None:intergenic 35.0%
CTCGATATGTAGATTAAGTG+AGG - chr2.2:11262336-11262355 None:intergenic 35.0%
CTTTGCATATTCAATCCAGT+TGG - chr2.2:11263610-11263629 None:intergenic 35.0%
GAATGTCAATATCTAACCTC+AGG - chr2.2:11263919-11263938 None:intergenic 35.0%
GACTAGGAATGAACTAATTG+AGG - chr2.2:11262214-11262233 None:intergenic 35.0%
GCTTCCTCTATAAAATTGGA+AGG - chr2.2:11263386-11263405 None:intergenic 35.0%
GGACACGATTGATAAAAATG+TGG + chr2.2:11262991-11263010 MS.gene059307:intron 35.0%
GGATTGAATATGCAAAGGAA+AGG + chr2.2:11263613-11263632 MS.gene059307:CDS 35.0%
GGGAGTGATTATTCTACTAA+TGG + chr2.2:11263353-11263372 MS.gene059307:CDS 35.0%
GGTAACAAAGAAGAGAGAAT+AGG - chr2.2:11260739-11260758 None:intergenic 35.0%
TAACCAGCTTGCAATTAACT+AGG - chr2.2:11264057-11264076 None:intergenic 35.0%
TCAGCAACACAAGTTATAGA+AGG + chr2.2:11263332-11263351 MS.gene059307:CDS 35.0%
TCAGCTTCATTCCAATTTGA+GGG + chr2.2:11261960-11261979 MS.gene059307:CDS 35.0%
TCTAACCTCAGGAAAAATAC+TGG - chr2.2:11263908-11263927 None:intergenic 35.0%
TGAATATGCAAAGGAAAGGT+TGG + chr2.2:11263617-11263636 MS.gene059307:CDS 35.0%
TGCAATTAGAAGCTCATAGT+GGG + chr2.2:11264692-11264711 MS.gene059307:CDS 35.0%
TGCTAAAGAAGACAACTGTA+AGG - chr2.2:11263741-11263760 None:intergenic 35.0%
TGGAATAAGACAGTGTGAAA+AGG - chr2.2:11264322-11264341 None:intergenic 35.0%
TTCAGCTTCATTCCAATTTG+AGG + chr2.2:11261959-11261978 MS.gene059307:CDS 35.0%
TTGCTTTCTCATGTGAATCT+TGG + chr2.2:11260792-11260811 MS.gene059307:CDS 35.0%
TTGGAATGAAGCTGAATTTC+GGG - chr2.2:11261955-11261974 None:intergenic 35.0%
! CAATTAGGTTCATATCGACT+AGG - chr2.2:11262230-11262249 None:intergenic 35.0%
! GTTCAACATAAAAGGAGCAT+TGG - chr2.2:11262529-11262548 None:intergenic 35.0%
! TAACAAGCTAGCTTATTAGC+TGG - chr2.2:11261559-11261578 None:intergenic 35.0%
! TAACACCAGTATTTTTCCTG+AGG + chr2.2:11263900-11263919 MS.gene059307:CDS 35.0%
! TATGCGTTGACAGGTTATAT+TGG + chr2.2:11262290-11262309 MS.gene059307:intron 35.0%
! TGTTTGCTAGGGGTAAATAT+GGG + chr2.2:11264788-11264807 MS.gene059307:intron 35.0%
! TTCTGGTGAATCATCACTTT+TGG - chr2.2:11261719-11261738 None:intergenic 35.0%
! TTGTTTGCTAGGGGTAAATA+TGG + chr2.2:11264787-11264806 MS.gene059307:intron 35.0%
! TTTCACACTGTCTTATTCCA+GGG + chr2.2:11264322-11264341 MS.gene059307:intron 35.0%
! TTTTTGCAGTACATGATAGG+GGG + chr2.2:11262799-11262818 MS.gene059307:intron 35.0%
!! ACATTCAATTTCTAGTGCCA+AGG + chr2.2:11263933-11263952 MS.gene059307:intron 35.0%
!! CAAGTTGGTTAAGCTTAGTT+AGG - chr2.2:11264113-11264132 None:intergenic 35.0%
!! CTTATTGTTGGCTTACCAAT+TGG + chr2.2:11263818-11263837 MS.gene059307:CDS 35.0%
!! GCTTGTTATGTTTCTGATGA+TGG + chr2.2:11261571-11261590 MS.gene059307:CDS 35.0%
!! GGATATGTTTTGTTAACAGC+AGG - chr2.2:11260860-11260879 None:intergenic 35.0%
!! TATTGTTGGCTTACCAATTG+GGG + chr2.2:11263820-11263839 MS.gene059307:CDS 35.0%
!! TTGTGAAGGTGTTGTTGATA+CGG - chr2.2:11260760-11260779 None:intergenic 35.0%
!! TTTTCACACTGTCTTATTCC+AGG + chr2.2:11264321-11264340 MS.gene059307:intron 35.0%
!!! TCGTCTTTGTGTTTTTTTGC+AGG + chr2.2:11261890-11261909 MS.gene059307:intron 35.0%
AATGATCGACGAAGTCACTT+TGG - chr2.2:11261214-11261233 None:intergenic 40.0%
AATTTGCAGACAAGAGTCTC+TGG + chr2.2:11262495-11262514 MS.gene059307:intron 40.0%
ACAACACCTTCACAATTCCA+TGG + chr2.2:11260765-11260784 MS.gene059307:CDS 40.0%
AGCAAGCATTCTCCTGTTAT+GGG + chr2.2:11263757-11263776 MS.gene059307:CDS 40.0%
AGTGACAGATAGAGATAGAG+CGG + chr2.2:11263051-11263070 MS.gene059307:intron 40.0%
ATTGGAGATGATGCAAATGC+TGG + chr2.2:11264577-11264596 MS.gene059307:CDS 40.0%
CAAAAGGAAAGTCCAACTTC+TGG + chr2.2:11264550-11264569 MS.gene059307:CDS 40.0%
CAACAAAAGAGATGGTCTTC+AGG + chr2.2:11263715-11263734 MS.gene059307:CDS 40.0%
CATGAGAAAGCAAGAAACCA+TGG - chr2.2:11260785-11260804 None:intergenic 40.0%
CGTTGACAGGTTATATTGGA+TGG + chr2.2:11262294-11262313 MS.gene059307:intron 40.0%
CTGCAAATTCTCCCTCAAAT+TGG - chr2.2:11261974-11261993 None:intergenic 40.0%
GCAAACAACCCTTTCAGAAA+TGG - chr2.2:11264775-11264794 None:intergenic 40.0%
GTACCTAGTTAATTGCAAGC+TGG + chr2.2:11264051-11264070 MS.gene059307:intron 40.0%
GTGCAATTAGAAGCTCATAG+TGG + chr2.2:11264691-11264710 MS.gene059307:CDS 40.0%
GTGTTCAATGTGATGATTGG+TGG - chr2.2:11261508-11261527 None:intergenic 40.0%
GTGTTTGATCCTCCATAGTA+TGG - chr2.2:11262844-11262863 None:intergenic 40.0%
GTTAACCATGTCCAAATTGG+TGG + chr2.2:11261285-11261304 MS.gene059307:intron 40.0%
TACCAACACATTCATAGCTC+CGG - chr2.2:11262394-11262413 None:intergenic 40.0%
TAGCAAGCATTCTCCTGTTA+TGG + chr2.2:11263756-11263775 MS.gene059307:CDS 40.0%
TATCCCTAAGAGCTTAAGCA+TGG - chr2.2:11264506-11264525 None:intergenic 40.0%
TATTGAAACTTCAGATGGGC+TGG + chr2.2:11263557-11263576 MS.gene059307:intron 40.0%
TCTCCAATAACACCAGAAGT+TGG - chr2.2:11264565-11264584 None:intergenic 40.0%
TGTTACTGGCCATTTCTGAA+AGG + chr2.2:11264763-11264782 MS.gene059307:CDS 40.0%
TTACATACCGTTGAAGGAGA+CGG - chr2.2:11260886-11260905 None:intergenic 40.0%
TTGGTGGAACAATCAGAGAA+TGG + chr2.2:11264435-11264454 MS.gene059307:CDS 40.0%
! CACTTACATTATGCGTTGAC+AGG + chr2.2:11262281-11262300 MS.gene059307:intron 40.0%
! CATACAGAGGGAGATTTTCT+TGG - chr2.2:11260658-11260677 None:intergenic 40.0%
! CTGAAGACCATCTCTTTTGT+TGG - chr2.2:11263717-11263736 None:intergenic 40.0%
! GAACACTGTTTTGTCTCTGT+TGG + chr2.2:11261522-11261541 MS.gene059307:CDS 40.0%
! GGAATAAAAGACGACCCTTT+TGG + chr2.2:11262669-11262688 MS.gene059307:CDS 40.0%
! GGTAAAATCCAATTTAGGGC+TGG + chr2.2:11263790-11263809 MS.gene059307:CDS 40.0%
! GTTACTGGCCATTTCTGAAA+GGG + chr2.2:11264764-11264783 MS.gene059307:CDS 40.0%
! TCTGAAAGGGTTGTTTGCTA+GGG + chr2.2:11264777-11264796 MS.gene059307:CDS 40.0%
! TTCTGAAAGGGTTGTTTGCT+AGG + chr2.2:11264776-11264795 MS.gene059307:CDS 40.0%
!! ACGACCCTTTTGGAAATCAA+TGG + chr2.2:11262679-11262698 MS.gene059307:CDS 40.0%
!! AGTCCAACTTCTGGTGTTAT+TGG + chr2.2:11264559-11264578 MS.gene059307:CDS 40.0%
!! ATGATTGGTGGTTCTAGAAC+AGG - chr2.2:11261496-11261515 None:intergenic 40.0%
!! CCACCCATTGATTTCCAAAA+GGG - chr2.2:11262686-11262705 None:intergenic 40.0%
!! CGACCCTTTTGGAAATCAAT+GGG + chr2.2:11262680-11262699 MS.gene059307:CDS 40.0%
!!! GGGTCACATCTTCCATTTTT+TGG - chr2.2:11261935-11261954 None:intergenic 40.0%
AAGACAAGCCAGCCCTAAAT+TGG - chr2.2:11263801-11263820 None:intergenic 45.0%
AGTGCCATGCTTAAGCTCTT+AGG + chr2.2:11264499-11264518 MS.gene059307:CDS 45.0%
ATACGCTTCGATCGTGATCA+TGG - chr2.2:11261185-11261204 None:intergenic 45.0%
ATCCAAACCGTCTCCTTCAA+CGG + chr2.2:11260876-11260895 MS.gene059307:CDS 45.0%
CTTCAAGGACCATACTATGG+AGG + chr2.2:11262832-11262851 MS.gene059307:CDS 45.0%
GAGATGATGCAAATGCTGGT+AGG + chr2.2:11264581-11264600 MS.gene059307:CDS 45.0%
GGACTTCAAGGACCATACTA+TGG + chr2.2:11262829-11262848 MS.gene059307:CDS 45.0%
GTCACAAACAAATCCACACG+TGG - chr2.2:11261463-11261482 None:intergenic 45.0%
GTGCCATGCTTAAGCTCTTA+GGG + chr2.2:11264500-11264519 MS.gene059307:CDS 45.0%
GTTAACAGCAGGACTCCATT+TGG - chr2.2:11260849-11260868 None:intergenic 45.0%
GTTGAACGTAGACTGTGACA+TGG + chr2.2:11262542-11262561 MS.gene059307:CDS 45.0%
TTGTGCCAACCACAAAAGCT+GGG - chr2.2:11264624-11264643 None:intergenic 45.0%
! AGTGGGAAAGAGTGTGGAAT+AGG + chr2.2:11264709-11264728 MS.gene059307:CDS 45.0%
! CAGAGGGAGATTTTCTTGGT+TGG - chr2.2:11260654-11260673 None:intergenic 45.0%
! CTGAAAGGGTTGTTTGCTAG+GGG + chr2.2:11264778-11264797 MS.gene059307:CDS 45.0%
! GACTTCGTCGATCATTGACT+TGG + chr2.2:11261218-11261237 MS.gene059307:intron 45.0%
! TAGCACTTGTTGGGGTAAAC+AGG + chr2.2:11263436-11263455 MS.gene059307:CDS 45.0%
!! AGATAGAGCGGTGTCACATA+TGG + chr2.2:11263063-11263082 MS.gene059307:intron 45.0%
!! CCCTTTTGGAAATCAATGGG+TGG + chr2.2:11262683-11262702 MS.gene059307:CDS 45.0%
!! CTTCAGATGGGCTGGTTATA+TGG + chr2.2:11263565-11263584 MS.gene059307:intron 45.0%
!! GCCACCCATTGATTTCCAAA+AGG - chr2.2:11262687-11262706 None:intergenic 45.0%
!!! ATTATATATCTTTATTTTAT+CGG + chr2.2:11262958-11262977 MS.gene059307:intron 5.0%
AGCCGGAGCTATGAATGTGT+TGG + chr2.2:11262389-11262408 MS.gene059307:CDS 50.0%
CCTGTTATGGGTACCCTCTT+TGG + chr2.2:11263769-11263788 MS.gene059307:CDS 50.0%
GCAGTACATGATAGGGGGTA+TGG + chr2.2:11262804-11262823 MS.gene059307:intron 50.0%
GGAAGCTCCTGTACTCTATG+AGG - chr2.2:11261442-11261461 None:intergenic 50.0%
GTTGTGCCAACCACAAAAGC+TGG - chr2.2:11264625-11264644 None:intergenic 50.0%
TACCAATTGGGGTCTGCGTT+CGG + chr2.2:11263831-11263850 MS.gene059307:CDS 50.0%
TACCGTTGAAGGAGACGGTT+TGG - chr2.2:11260881-11260900 None:intergenic 50.0%
TCAATGGGTGGCTGCATTTG+AGG + chr2.2:11262695-11262714 MS.gene059307:CDS 50.0%
TGTGCCAACCACAAAAGCTG+GGG - chr2.2:11264623-11264642 None:intergenic 50.0%
! ACTGTCTTATTCCAGGGAGC+TGG + chr2.2:11264328-11264347 MS.gene059307:intron 50.0%
! CTGTCTTATTCCAGGGAGCT+GGG + chr2.2:11264329-11264348 MS.gene059307:intron 50.0%
! GCTCATAGTGGGAAAGAGTG+TGG + chr2.2:11264703-11264722 MS.gene059307:CDS 50.0%
! TACATGATAGGGGGTATGGC+AGG + chr2.2:11262808-11262827 MS.gene059307:CDS 50.0%
! TGATGAGTCCCCAGCTTTTG+TGG + chr2.2:11264612-11264631 MS.gene059307:CDS 50.0%
!! CCAAAGAGGGTACCCATAAC+AGG - chr2.2:11263772-11263791 None:intergenic 50.0%
!! GGGCTGGCTTGTCTTATTGT+TGG + chr2.2:11263806-11263825 MS.gene059307:CDS 50.0%
AGTACAGGAGCTTCCACGTG+TGG + chr2.2:11261447-11261466 MS.gene059307:CDS 55.0%
TATTCCAGGGAGCTGGGCTA+TGG + chr2.2:11264335-11264354 MS.gene059307:intron 55.0%
! GAGTCCCCAGCTTTTGTGGT+TGG + chr2.2:11264616-11264635 MS.gene059307:CDS 55.0%
GAATCCATAGCCCAGCTCCC+TGG - chr2.2:11264342-11264361 None:intergenic 60.0%
GACCGAACGCAGACCCCAAT+TGG - chr2.2:11263836-11263855 None:intergenic 60.0%
GCACAGCCTGTAAACGCGGT+TGG - chr2.2:11263667-11263686 None:intergenic 60.0%
GCGTTTACAGGCTGTGCTCC+CGG + chr2.2:11263670-11263689 MS.gene059307:CDS 60.0%
TTTACAGGCTGTGCTCCCGG+AGG + chr2.2:11263673-11263692 MS.gene059307:CDS 60.0%
! CCTGTAAACGCGGTTGGCTC+GGG - chr2.2:11263661-11263680 None:intergenic 60.0%
! GCCTGTAAACGCGGTTGGCT+CGG - chr2.2:11263662-11263681 None:intergenic 60.0%
! GGGAGCACAGCCTGTAAACG+CGG - chr2.2:11263671-11263690 None:intergenic 60.0%
! GGGGGTATGGCAGGACTTCA+AGG + chr2.2:11262817-11262836 MS.gene059307:CDS 60.0%
CCCGAGCCAACCGCGTTTAC+AGG + chr2.2:11263658-11263677 MS.gene059307:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 11260647 11264886 11260647 ID=MS.gene059307
chr2.2 mRNA 11260647 11264886 11260647 ID=MS.gene059307.t1;Parent=MS.gene059307
chr2.2 exon 11260647 11260897 11260647 ID=MS.gene059307.t1.exon1;Parent=MS.gene059307.t1
chr2.2 CDS 11260647 11260897 11260647 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11261447 11261765 11261447 ID=MS.gene059307.t1.exon2;Parent=MS.gene059307.t1
chr2.2 CDS 11261447 11261765 11261447 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11261912 11262037 11261912 ID=MS.gene059307.t1.exon3;Parent=MS.gene059307.t1
chr2.2 CDS 11261912 11262037 11261912 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11262303 11262410 11262303 ID=MS.gene059307.t1.exon4;Parent=MS.gene059307.t1
chr2.2 CDS 11262303 11262410 11262303 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11262504 11262716 11262504 ID=MS.gene059307.t1.exon5;Parent=MS.gene059307.t1
chr2.2 CDS 11262504 11262716 11262504 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11262808 11262925 11262808 ID=MS.gene059307.t1.exon6;Parent=MS.gene059307.t1
chr2.2 CDS 11262808 11262925 11262808 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11263273 11263457 11263273 ID=MS.gene059307.t1.exon7;Parent=MS.gene059307.t1
chr2.2 CDS 11263273 11263457 11263273 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11263571 11263921 11263571 ID=MS.gene059307.t1.exon8;Parent=MS.gene059307.t1
chr2.2 CDS 11263571 11263921 11263571 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11264343 11264799 11264343 ID=MS.gene059307.t1.exon9;Parent=MS.gene059307.t1
chr2.2 CDS 11264343 11264799 11264343 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
chr2.2 exon 11264873 11264886 11264873 ID=MS.gene059307.t1.exon10;Parent=MS.gene059307.t1
chr2.2 CDS 11264873 11264886 11264873 ID=cds.MS.gene059307.t1;Parent=MS.gene059307.t1
Gene Sequence

>MS.gene059307

ATGGCCAACCAAGAAAATCTCCCTCTGTATGAAAAAATATGGATCAAACGAACATATCAAAGAGCAATAGATATCTTGATCTTACTCCTCCTATTCTCTCTTCTTTGTTACCGTATCAACAACACCTTCACAATTCCATGGTTTCTTGCTTTCTCATGTGAATCTTGGTTCACATACACTTGGATTATTCTTCTCAATACCAAATGGAGTCCTGCTGTTAACAAAACATATCCAAACCGTCTCCTTCAACGGTATGTAATATAAACTGTCGCGTCGCAATATTTATTATTATTATAAATTGCTAACAAATGTATGTAATTGATTGATGTGACTCAATCAATAGTTATGGCGACATCAAGATTGATATCAAGAATCTTGATGTCTCAGTTTATATGTCGCGGTTTAAGCATTTTTTTTTTTTTATTGCATTGACATCGATTTTGTATTGCAATTCTATTATGATATTTAAACAATTAAATCTAGACAATTTTCTTGACAACGTTTTAATAAAGTTCCGTAGATGATAATAGATATACCATGATCACGATCGAAGCGTATTTATCACCAAAGTGACTTCGTCGATCATTGACTTGGATGTGATTAACTACGTACATTTAGTTGAAAACTTGAAAATTATGGTTAACCATGTCCAAATTGGTGGTTAATGAGTTTTATATCCTTATCTTTAATCTGCATTAACCCCTTAAAAAGTTGTCTAAATTTGGTTGTAAAAACCATTTATCTTGCTAATTAGTTTCTCAAGTATTGTGCTAATTTTTTGTTATTTTTTATCCTCATAGAGTACAGGAGCTTCCACGTGTGGATTTGTTTGTGACAACAGCAGATCCTGTTCTAGAACCACCAATCATCACATTGAACACTGTTTTGTCTCTGTTGGCTCTTGATTATCCAGCTAATAAGCTAGCTTGTTATGTTTCTGATGATGGCTGTTCTGTTTTTACCTTTTATGGTCTTTTGGAAGCTTCCAAATTTGCTAAGTTTTGGGTTCCTTTCTGTAAGAAGTATAATATACAAGTTAGAGCACCTTTTAGATATTTCTCTCAAGTTACCAAAAGTGATGATTCACCAGAATTCAAACAAGAATGGTTACAAATGAAGGTAAGTAAAATAATATTACGCTTCAACTATTGATACATGTTATGAGATAAGTATCTAAATCTATGGTGGCATTTAGCATGCACAAGTAACAAATTTGTAGGATGGTACGTGAACCATTGATGATTCGTCTTTGTGTTTTTTTGCAGGATATGTATGACAATCTTAGCCAAAAAATGGAAGATGTGACCCGAAATTCAGCTTCATTCCAATTTGAGGGAGAATTTGCAGTATTCTCAAATACAGAAAAAAGGAATCATCCAAGTATAATTAAGGTAATAAATATTGCAGTAAAAATATCAAATGCATCTATAGAGTACATGTTTTCCTTGTTCTATAATTGAACAAATTTTAGCAGGTAGAATTTAATTTAATATATACACACATCCTAAATTCTTCCAAGTATTTCTGGTTCTTGCATTCTAAAATGTTTTGAGTGTTTGTGTCACCTCAATTAGTTCATTCCTAGTCGATATGAACCTAATTGACTCACACATGAGTTCTAACAAAACAAAAAACACTTACATTATGCGTTGACAGGTTATATTGGATGGTCATTCTGATGGATTGCCTCACTTAATCTACATATCGAGAGAGAAGAGACCAAATTATGAACACAATTACAAAGCCGGAGCTATGAATGTGTTGGTAATTTCGCTGACCAATATTATTTAGCTCTGCGAAATACTAATTCTTATGTTTCCGAGACTCATTCTTATCTTTCTTAACGGGAATTTGCAGACAAGAGTCTCTGGATTGATGACCAATGCTCCTTTTATGTTGAACGTAGACTGTGACATGGTTGTGAACAATCCCAAGATTATGCAACATGCTATTTGCATATTGATGGATTCTAAGAACGGAAAAGATGTTGCTTTTGTTCAATGTTTCCAAAAATTTTACGATGGAATAAAAGACGACCCTTTTGGAAATCAATGGGTGGCTGCATTTGAGGTTTTATCTCAACTTCAAATGCATTTCTTACTTCACTACGTACCACTTATAAATTTGTGTTTGTAAACAAATTTTGTTTTGTTTTTTGCAGTACATGATAGGGGGTATGGCAGGACTTCAAGGACCATACTATGGAGGATCAAACACCTTCCACAGAAGATATGCTATTTACGGTTTTTATCCTAATCAAATACAATATGGAAATAAAGGTCTTGCTCTCTCATAAGAAATTCACTTATTCATTATATATCTTTATTTTATCGGAATGTGATATGGACACGATTGATAAAAATGTGGTGCGTAATCTGTGTAAGTTGTGAAAGATGAAAAATTAAGTGACAGATAGAGATAGAGCGGTGTCACATATGGTGTCAAACTCGTGTTCTTTCGAATGATTTGTCCTTAACTAGAACCAATTAACAACTTAAGCTCAATCACTTGCTTAAATTACAATATGCTTGAGTAATTATATGAGGATAGTATTAGTATTAGTATTAGTATTAATAGTTGATTCCTGTATGATACTAAAAAACTCGCTATTTTGTATCTTTACTAGGAAAATTAGCCGAGAATGTACTAATACAACAATTTGGAAGTTCAAAGGAGTTTATCAAATCAGCAACACAAGTTATAGAAGGGAGTGATTATTCTACTAATGGTATTAGTCCTTCCAATTTTATAGAGGAAGCAATCCAAGTATCTGATTGTGAATATGAATATAGCACTTGTTGGGGTAAACAGGTATATTTTTCAGTACTTGCAAAATATAAATTTTGTGAATAAGCCTTAGACTATAAAATTACACAAATTTTTAAAAAATGCATATGAAACTTGATTATCTATTGAAACTTCAGATGGGCTGGTTATATGGATCAATATCAGAAGATGTACCAACTGGATTGAATATGCAAAGGAAAGGTTGGAGATCAGAATGTTGTACACCCGAGCCAACCGCGTTTACAGGCTGTGCTCCCGGAGGATTACTTACTACAATGATCCAACAAAAGAGATGGTCTTCAGGCCTTACAGTTGTCTTCTTTAGCAAGCATTCTCCTGTTATGGGTACCCTCTTTGGTAAAATCCAATTTAGGGCTGGCTTGTCTTATTGTTGGCTTACCAATTGGGGTCTGCGTTCGGTCTTTGAAGTTTCTTATGCAGCCCTAGTTGCATATTGCATAATCACTAACACCAGTATTTTTCCTGAGGTTAGATATTGACATTCAATTTCTAGTGCCAAGGTTAAATTAAACATTGAGCATAACAGCTGTATGCATCGACAGTATAAAAAAATTACACTCGCATTTAATCATAAAATCAGTTGGAGTTTGTTAAATGTACCTAGTTAATTGCAAGCTGGTTACAGTGAATGTAGAATACAATTTTATTTAGTGATCCTAACTAAGCTTAACCAACTTGCAAGTTGCAACTAATTATAACCAATCTCCGACTAATTTTAACAGTTTTTGGAAATAATATAACAACGGTTAGGTTCGTATTGTGATATATAATTAAACGCACGTGTAAAACTTATTTGTCGTGCATAGTCGTTAAAATCTTACTAAAGATCTCACTAATTATGAGCTTGATATACTACTTTTTTCTCCTTTTCACACTGTCTTATTCCAGGGAGCTGGGCTATGGATTCCTTTGACTCTTTTTGTGATCTATACTATGCATACTCTACAAGAGTATAAAGTAAAAGGGTTTTCATTAAGATATTGGTGGAACAATCAGAGAATGGTTACAATAAGATCAACAAGTGTATGGTTTATAGGATTTTTGAGTGCCATGCTTAAGCTCTTAGGGATATCTGATACAGTCTTTGAAGTAACACAAAAGGAAAGTCCAACTTCTGGTGTTATTGGAGATGATGCAAATGCTGGTAGGTTCACATTTGATGAGTCCCCAGCTTTTGTGGTTGGCACAACCATTTTGCTTGTGCAGTTGACAGCACTTGTTATTAAGATTTTGGGGGTGCAATTAGAAGCTCATAGTGGGAAAGAGTGTGGAATAGGTGAATTGATGTGTAGTGTGTATCTAGTAGTTTGTTACTGGCCATTTCTGAAAGGGTTGTTTGCTAGGGGTAAATATGGGATTCCCTTATCTACCATTTTCAAGTCAGCACTTTTTGCTTTAATTTTTGTGCACTTTTGTAGAATTACTCCTATAA
Protein sequence

>MS.gene059307.t1

MANQENLPLYEKIWIKRTYQRAIDILILLLLFSLLCYRINNTFTIPWFLAFSCESWFTYTWIILLNTKWSPAVNKTYPNRLLQRVQELPRVDLFVTTADPVLEPPIITLNTVLSLLALDYPANKLACYVSDDGCSVFTFYGLLEASKFAKFWVPFCKKYNIQVRAPFRYFSQVTKSDDSPEFKQEWLQMKDMYDNLSQKMEDVTRNSASFQFEGEFAVFSNTEKRNHPSIIKVILDGHSDGLPHLIYISREKRPNYEHNYKAGAMNVLTRVSGLMTNAPFMLNVDCDMVVNNPKIMQHAICILMDSKNGKDVAFVQCFQKFYDGIKDDPFGNQWVAAFEYMIGGMAGLQGPYYGGSNTFHRRYAIYGFYPNQIQYGNKGKLAENVLIQQFGSSKEFIKSATQVIEGSDYSTNGISPSNFIEEAIQVSDCEYEYSTCWGKQMGWLYGSISEDVPTGLNMQRKGWRSECCTPEPTAFTGCAPGGLLTTMIQQKRWSSGLTVVFFSKHSPVMGTLFGKIQFRAGLSYCWLTNWGLRSVFEVSYAALVAYCIITNTSIFPEGAGLWIPLTLFVIYTMHTLQEYKVKGFSLRYWWNNQRMVTIRSTSVWFIGFLSAMLKLLGISDTVFEVTQKESPTSGVIGDDANAGRFTFDESPAFVVGTTILLVQLTALVIKILGVQLEAHSGKECGIGELMCSVYLVVCYWPFLKGLFARELLL