AlfalfaGEDB | MS.gene059306

M. sativa cultivar XinJiangDaYe / MS.gene059306

KEGG: -
GO: GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009250 GO:0009987 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0016760 GO:0030243 GO:0030244 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0046527 GO:0051273 GO:0051274 GO:0071704 GO:0110165 GO:1901576
PFAM: PF03552.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059306.t1	XP_003596946.2	76.6	615	31	2	40	541	84	698	3.30E-264	920.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059306.t1	Q339N5	51.3	431	204	4	49	475	89	517	3.2e-122	440.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059306.t1	G7ITV3	76.6	615	31	2	40	541	84	698	2.4e-264	920.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052176	MS.gene059306	0.802573	5.26E-49	-1.69E-46
MS.gene052190	MS.gene059306	0.813005	3.19E-51	-1.69E-46
MS.gene052333	MS.gene059306	0.813586	2.38E-51	-1.69E-46
MS.gene053166	MS.gene059306	0.813051	3.11E-51	-1.69E-46
MS.gene054468	MS.gene059306	0.80655	7.79E-50	-1.69E-46
MS.gene054559	MS.gene059306	0.800322	1.52E-48	-1.69E-46
MS.gene056506	MS.gene059306	0.832379	9.78E-56	-1.69E-46
MS.gene057200	MS.gene059306	0.803716	3.05E-49	-1.69E-46
MS.gene058979	MS.gene059306	0.810357	1.20E-50	-1.69E-46
MS.gene059306	MS.gene060683	0.800544	1.37E-48	-1.69E-46
MS.gene059306	MS.gene060685	0.801975	6.99E-49	-1.69E-46
MS.gene059306	MS.gene06698	0.800791	1.22E-48	-1.69E-46
MS.gene059306	MS.gene068526	0.820703	5.96E-53	-1.69E-46
MS.gene059306	MS.gene072677	0.800464	1.42E-48	-1.69E-46
MS.gene059306	MS.gene073853	0.819889	9.16E-53	-1.69E-46
MS.gene059306	MS.gene09952	0.8051	1.57E-49	-1.69E-46
MS.gene059306	MS.gene21149	0.813266	2.79E-51	-1.69E-46
MS.gene059306	MS.gene31283	0.805861	1.09E-49	-1.69E-46
MS.gene059306	MS.gene31397	0.804904	1.73E-49	-1.69E-46
MS.gene059306	MS.gene32874	0.81445	1.53E-51	-1.69E-46
MS.gene059306	MS.gene35708	0.818659	1.75E-52	-1.69E-46
MS.gene059306	MS.gene38381	0.813034	3.14E-51	-1.69E-46
MS.gene059306	MS.gene44603	0.818758	1.66E-52	-1.69E-46
MS.gene059306	MS.gene47050	0.819345	1.22E-52	-1.69E-46
MS.gene059306	MS.gene48435	0.802348	5.86E-49	-1.69E-46
MS.gene059306	MS.gene52059	0.81218	4.83E-51	-1.69E-46
MS.gene059306	MS.gene56537	0.801357	9.36E-49	-1.69E-46
MS.gene059306	MS.gene58503	0.805622	1.22E-49	-1.69E-46
MS.gene059306	MS.gene60204	0.820528	6.54E-53	-1.69E-46
MS.gene059306	MS.gene60206	0.823687	1.21E-53	-1.69E-46
MS.gene059306	MS.gene61139	0.823288	1.50E-53	-1.69E-46
MS.gene059306	MS.gene69794	0.811996	5.29E-51	-1.69E-46
MS.gene059306	MS.gene71871	0.800816	1.21E-48	-1.69E-46
MS.gene059306	MS.gene73820	0.815396	9.45E-52	-1.69E-46
MS.gene059306	MS.gene75490	0.807315	5.37E-50	-1.69E-46
MS.gene059306	MS.gene79024	0.809245	2.08E-50	-1.69E-46
MS.gene059306	MS.gene83734	0.810283	1.25E-50	-1.69E-46
MS.gene059306	MS.gene86434	0.816609	5.06E-52	-1.69E-46
MS.gene059306	MS.gene93041	0.853021	3.15E-61	-1.69E-46
MS.gene059306	MS.gene95377	0.825574	4.35E-54	-1.69E-46
MS.gene059306	MS.gene95552	0.804841	1.78E-49	-1.69E-46
MS.gene059306	MS.gene98326	0.802309	5.97E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene35707	MS.gene059306	PPI
MS.gene059306	MS.gene35707	PPI
MS.gene059306	MS.gene059307	PPI
MS.gene059304	MS.gene059306	PPI
MS.gene35839	MS.gene059306	PPI
MS.gene059306	MS.gene061307	PPI
MS.gene66611	MS.gene059306	PPI
MS.gene61615	MS.gene059306	PPI
MS.gene034349	MS.gene059306	PPI
MS.gene059306	MS.gene034349	PPI
MS.gene059306	MS.gene35708	PPI
MS.gene059306	MS.gene35839	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059306.t1	MTR_2g087960	94.508	437	23	1	40	475	84	520	0.0	871
MS.gene059306.t1	MTR_2g087960	87.879	66	8	0	476	541	633	698	5.46e-29	122
MS.gene059306.t1	MTR_2g087960	94.508	437	23	1	40	475	84	520	0.0	868
MS.gene059306.t1	MTR_2g087980	86.215	428	55	2	49	475	86	510	0.0	788
MS.gene059306.t1	MTR_2g087980	90.909	66	6	0	476	541	623	688	1.21e-29	124
MS.gene059306.t1	MTR_2g087900	60.090	446	164	5	39	474	82	523	0.0	561
MS.gene059306.t1	MTR_2g087900	60.090	446	164	5	39	474	82	523	0.0	561
MS.gene059306.t1	MTR_4g050000	62.326	430	152	4	49	474	88	511	0.0	555
MS.gene059306.t1	MTR_4g050000	52.239	67	31	1	476	541	625	691	1.92e-11	67.4
MS.gene059306.t1	MTR_2g087950	62.844	436	152	5	48	477	87	518	0.0	550
MS.gene059306.t1	MTR_2g087900	58.779	393	147	6	39	421	82	469	1.48e-162	471
MS.gene059306.t1	MTR_2g087900	66.165	266	87	2	39	302	82	346	2.77e-129	381
MS.gene059306.t1	MTR_8g086600	39.958	478	230	9	51	474	272	746	1.22e-106	342
MS.gene059306.t1	MTR_4g130510	40.842	475	228	9	49	474	325	795	2.55e-106	338
MS.gene059306.t1	MTR_2g016630	38.923	483	233	7	50	474	256	734	2.71e-105	338
MS.gene059306.t1	MTR_4g130510	40.842	475	228	9	49	474	325	795	8.41e-105	338
MS.gene059306.t1	MTR_3g060240	39.908	436	228	6	49	474	92	503	2.04e-104	330
MS.gene059306.t1	MTR_3g060220	39.817	437	228	6	48	474	92	503	4.40e-104	329
MS.gene059306.t1	MTR_3g060220	39.817	437	228	6	48	474	91	502	4.53e-104	329
MS.gene059306.t1	MTR_3g060280	38.813	438	227	4	48	474	101	508	5.03e-103	322
MS.gene059306.t1	MTR_3g060280	38.813	438	227	4	48	474	101	508	1.00e-102	321
MS.gene059306.t1	MTR_8g063270	39.270	466	242	8	47	474	69	531	1.47e-101	323
MS.gene059306.t1	MTR_3g060280	38.826	443	220	5	48	474	101	508	5.91e-101	321
MS.gene059306.t1	MTR_8g063270	39.270	466	242	8	47	474	274	736	5.54e-100	324
MS.gene059306.t1	MTR_1g098550	36.970	495	237	9	50	474	366	855	7.82e-99	323
MS.gene059306.t1	MTR_1g098550	36.970	495	237	9	50	474	362	851	8.11e-99	323
MS.gene059306.t1	MTR_1g069605	39.066	471	238	8	49	474	185	651	1.78e-98	318
MS.gene059306.t1	MTR_4g130510	38.114	509	228	10	49	474	325	829	3.02e-98	321
MS.gene059306.t1	MTR_3g107520	36.917	493	240	9	49	474	355	843	1.70e-95	313
MS.gene059306.t1	MTR_5g029190	34.815	540	239	15	48	476	346	883	5.88e-91	302
MS.gene059306.t1	MTR_1g073740	37.808	447	233	8	45	475	93	510	4.73e-89	290
MS.gene059306.t1	MTR_4g088225	37.215	438	250	7	45	475	92	511	5.58e-89	289
MS.gene059306.t1	MTR_1g039480	35.065	539	234	16	48	472	353	889	8.38e-89	296
MS.gene059306.t1	MTR_4g088225	37.215	438	250	7	45	475	92	511	1.03e-88	288
MS.gene059306.t1	MTR_1g039445	33.948	542	251	14	36	472	322	861	4.44e-88	294
MS.gene059306.t1	MTR_0011s0020	37.209	430	244	7	47	473	82	488	6.72e-87	283
MS.gene059306.t1	MTR_4g077910	34.259	540	242	18	48	476	367	904	2.51e-86	289
MS.gene059306.t1	MTR_2g037830	36.636	434	244	8	46	473	80	488	4.06e-84	275
MS.gene059306.t1	MTR_7g109200	33.028	545	245	14	48	476	391	931	4.15e-84	283
MS.gene059306.t1	MTR_3g450800	34.607	445	261	7	34	473	20	439	1.35e-82	270
MS.gene059306.t1	MTR_3g005560	35.402	435	250	7	45	473	79	488	1.51e-82	268
MS.gene059306.t1	MTR_4g130510	40.000	405	190	9	49	404	325	725	1.76e-82	272
MS.gene059306.t1	MTR_2g438220	32.584	534	251	14	48	474	253	784	6.38e-82	276
MS.gene059306.t1	MTR_2g037830	36.538	416	233	8	46	455	80	470	2.51e-81	264
MS.gene059306.t1	MTR_3g005560	35.402	435	250	7	45	473	79	488	4.15e-81	267
MS.gene059306.t1	MTR_3g060280	36.658	371	194	4	48	407	101	441	1.91e-79	256
MS.gene059306.t1	MTR_7g116230	34.394	503	231	16	48	475	223	701	3.54e-77	260
MS.gene059306.t1	MTR_2g035780	46.488	299	148	5	49	336	296	593	1.29e-75	258
MS.gene059306.t1	MTR_2g035780	37.500	128	77	1	347	474	678	802	3.17e-20	95.5
MS.gene059306.t1	MTR_8g092590	44.595	296	152	5	49	333	363	657	2.30e-75	258
MS.gene059306.t1	MTR_8g092590	42.759	145	73	2	337	474	713	854	1.62e-25	112
MS.gene059306.t1	MTR_3g030040	43.919	296	154	6	49	333	345	639	2.19e-74	255
MS.gene059306.t1	MTR_3g030040	39.062	128	75	1	347	474	711	835	1.48e-21	99.8
MS.gene059306.t1	MTR_8g037260	43.478	299	157	6	49	336	345	642	1.82e-73	253
MS.gene059306.t1	MTR_8g037260	38.636	132	78	1	343	474	707	835	5.13e-22	100
MS.gene059306.t1	MTR_7g106680	43.243	296	156	6	49	333	303	597	4.97e-72	248
MS.gene059306.t1	MTR_7g106680	37.879	132	79	1	343	474	661	789	4.23e-23	104
MS.gene059306.t1	MTR_3g007770	44.444	297	153	6	49	334	312	607	8.88e-71	245
MS.gene059306.t1	MTR_3g007770	40.625	128	73	2	347	474	679	803	6.12e-21	97.8
MS.gene059306.t1	MTR_2g087900	62.963	162	58	1	39	198	82	243	1.64e-68	223
MS.gene059306.t1	MTR_3g005560	35.326	368	207	7	45	406	79	421	1.66e-66	222
MS.gene059306.t1	MTR_3g091270	33.716	436	191	14	48	428	104	496	6.79e-58	201

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059306.t1	AT2G32540	49.657	437	203	6	49	475	90	519	4.03e-143	431
MS.gene059306.t1	AT2G32530	49.199	437	205	5	49	475	90	519	2.54e-142	429
MS.gene059306.t1	AT2G32620	49.195	435	200	6	50	472	91	516	6.83e-141	418
MS.gene059306.t1	AT2G32620	49.087	438	202	6	50	475	91	519	7.03e-141	420
MS.gene059306.t1	AT2G32620	49.087	438	202	6	50	475	91	519	4.10e-139	421
MS.gene059306.t1	AT4G15320	47.500	440	213	6	49	478	90	521	2.08e-138	419
MS.gene059306.t1	AT4G15320	47.500	440	213	6	49	478	90	521	2.34e-138	419
MS.gene059306.t1	AT2G32620	48.843	432	200	6	56	475	1	423	8.01e-137	411
MS.gene059306.t1	AT4G15290	46.429	448	218	8	39	475	82	518	9.89e-136	412
MS.gene059306.t1	AT2G32610	46.224	437	217	6	49	474	90	519	5.17e-135	410
MS.gene059306.t1	AT4G15320	45.934	455	213	7	49	478	90	536	7.11e-135	410
MS.gene059306.t1	AT4G15320	43.004	486	213	7	49	478	90	567	8.50e-130	397
MS.gene059306.t1	AT4G15320	39.698	529	212	9	49	478	90	610	5.70e-121	375
MS.gene059306.t1	AT4G18780	39.754	488	229	8	48	474	257	740	4.29e-107	343
MS.gene059306.t1	AT5G17420	40.546	476	229	8	49	474	312	783	6.95e-107	343
MS.gene059306.t1	AT5G09870	39.919	496	222	10	49	474	338	827	1.11e-106	343
MS.gene059306.t1	AT5G64740	38.945	493	230	10	51	474	352	842	9.38e-104	336
MS.gene059306.t1	AT2G21770	38.415	492	235	9	49	474	356	845	5.67e-101	328
MS.gene059306.t1	AT5G05170	37.449	494	237	8	49	474	333	822	2.32e-100	327
MS.gene059306.t1	AT1G55850	40.278	432	215	7	49	474	106	500	8.47e-98	312
MS.gene059306.t1	AT4G32410	37.143	490	243	9	49	474	349	837	2.47e-96	316
MS.gene059306.t1	AT2G25540	37.323	493	238	11	49	474	329	817	5.34e-95	312
MS.gene059306.t1	AT4G38190	33.829	538	255	16	37	476	334	868	1.22e-86	290
MS.gene059306.t1	AT1G32180	34.254	543	239	15	48	474	200	740	1.57e-85	285
MS.gene059306.t1	AT1G32180	34.254	543	239	15	48	474	200	740	1.57e-85	285
MS.gene059306.t1	AT3G03050	33.895	534	242	16	48	472	372	903	1.35e-84	285
MS.gene059306.t1	AT4G23990	36.854	445	229	10	47	474	114	523	1.37e-84	278
MS.gene059306.t1	AT4G24010	37.303	445	227	10	47	474	95	504	4.23e-84	277
MS.gene059306.t1	AT4G24000	37.757	437	229	10	47	474	92	494	1.89e-83	274
MS.gene059306.t1	AT5G16910	33.271	535	249	16	48	476	375	907	2.11e-81	276
MS.gene059306.t1	AT1G02730	32.969	549	243	15	48	474	396	941	4.49e-81	275
MS.gene059306.t1	AT2G33100	31.227	538	255	13	48	472	261	796	4.37e-78	265
MS.gene059306.t1	AT2G33100	31.227	538	255	13	48	472	261	796	4.37e-78	265
MS.gene059306.t1	AT5G44030	45.333	300	152	6	48	336	9	307	4.13e-76	256
MS.gene059306.t1	AT5G44030	37.681	138	81	2	337	474	394	526	8.83e-22	100
MS.gene059306.t1	AT4G39350	45.085	295	150	5	51	334	353	646	6.11e-76	260
MS.gene059306.t1	AT4G39350	41.667	132	74	1	343	474	713	841	1.19e-23	106
MS.gene059306.t1	AT5G44030	45.333	300	152	6	48	336	288	586	1.21e-74	256
MS.gene059306.t1	AT5G44030	37.681	138	81	2	337	474	673	805	1.43e-21	99.8
MS.gene059306.t1	AT5G44030	45.333	300	152	6	48	336	317	615	1.98e-74	256
MS.gene059306.t1	AT5G44030	37.681	138	81	2	337	474	702	834	1.20e-21	99.8
MS.gene059306.t1	AT4G24000	34.650	329	178	6	155	474	2	302	4.35e-50	181
MS.gene059306.t1	AT4G23990	33.828	337	177	8	155	474	2	309	2.89e-49	178

Find 104 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCACTTGTTATTAAGATTT+TGG	0.081874	2.2:+11289358	MS.gene059306:CDS
TACCCAAAACTTAGCAAATT+TGG	0.129500	2.2:-11286391	None:intergenic
CTTCCAAATTTGCTAAGTTT+TGG	0.203327	2.2:+11286388	MS.gene059306:CDS
CATCAGATGGGTTGGTTATA+TGG	0.220657	2.2:+11288146	MS.gene059306:intron
GGAATTAAAGATGACCCTTT+TGG	0.230566	2.2:+11287336	MS.gene059306:CDS
AGCAAGCATTCTCCTGTTAT+AGG	0.236176	2.2:+11288338	MS.gene059306:CDS
AACCATTCTTGTTTAAATTC+TGG	0.253368	2.2:-11286492	None:intergenic
AAGATATTAATACAGCAATT+TGG	0.270520	2.2:+11287696	MS.gene059306:CDS
CTGCATATTCTCCCTCAAAT+TGG	0.301654	2.2:-11286678	None:intergenic
CTTTATGCAGTACATAATAA+AGG	0.304711	2.2:+11287463	MS.gene059306:intron
GCTTGTTATGTTTCTGATGA+TGG	0.315570	2.2:+11286330	MS.gene059306:CDS
GCCTTCATGGGCTGCGCTCC+CGG	0.320849	2.2:+11288251	MS.gene059306:CDS
AAGACGGTTTGGATATGTTT+TGG	0.323944	2.2:-11285654	None:intergenic
TATCCTGATGAAATTCAATA+TGG	0.325981	2.2:+11287560	MS.gene059306:CDS
GGACTTCAAGGACCTTTCTA+TGG	0.332996	2.2:+11287494	MS.gene059306:CDS
TTCTGGTGAATCTTCACTAT+TGG	0.337945	2.2:-11286475	None:intergenic
AAAGCGTTGACAGGTTATAT+TGG	0.339961	2.2:+11286951	MS.gene059306:intron
TCAGATTGTGGATATGAATA+TGG	0.340911	2.2:+11287825	MS.gene059306:CDS
CAGTCTATGTTCAACATAAA+AGG	0.347327	2.2:-11287201	None:intergenic
ACCGGAGCCAACCGCCTTCA+TGG	0.356349	2.2:+11288238	MS.gene059306:CDS
CACCAGAATTTAAACAAGAA+TGG	0.390854	2.2:+11286490	MS.gene059306:CDS
CCCATGAAGGCGGTTGGCTC+CGG	0.391178	2.2:-11288239	None:intergenic
CATAGCATGTTGAATAATCT+TGG	0.402560	2.2:-11287244	None:intergenic
TGGCACTTGTTGGGGTAAAC+AGG	0.403166	2.2:+11287845	MS.gene059306:CDS
CATATCCACAATCTGATACT+TGG	0.404400	2.2:-11287818	None:intergenic
CTTCCATATTGAATTTCATC+AGG	0.404640	2.2:-11287563	None:intergenic
CATAGAAGAAATGCTATATA+TGG	0.408572	2.2:+11287533	MS.gene059306:CDS
TAATTCCATCATAAAATTGT+TGG	0.409034	2.2:-11287320	None:intergenic
TTCAACTTCATTCCAATTTG+AGG	0.413057	2.2:+11286666	MS.gene059306:CDS
TTTATGCAGTACATAATAAA+GGG	0.413341	2.2:+11287464	MS.gene059306:intron
CCAAAGAGGGTACCTATAAC+AGG	0.415661	2.2:-11288350	None:intergenic
GAAATTCAATATGGAAGAAA+AGG	0.424433	2.2:+11287569	MS.gene059306:CDS
CAACAAAAGAGATGGTCTTC+AGG	0.429438	2.2:+11288296	MS.gene059306:CDS
TCAACTTCATTCCAATTTGA+GGG	0.436853	2.2:+11286667	MS.gene059306:CDS
TACCGTTGAAGAAGACGGTT+TGG	0.445224	2.2:-11285665	None:intergenic
TTGTAATTGTGTTCGTACTT+TGG	0.447431	2.2:-11287036	None:intergenic
TGAATATGCAAAGAAAAGGT+TGG	0.448456	2.2:+11288198	MS.gene059306:CDS
GGGAGTGATAATTCAATTGA+TGG	0.450914	2.2:+11287762	MS.gene059306:CDS
GATTGCTTTATCAAGAAGAA+TGG	0.454022	2.2:-11287796	None:intergenic
GTTGTGCCAACCACAAAAGC+TGG	0.457175	2.2:-11289314	None:intergenic
AAATCATTCAAGTATAGTTA+AGG	0.459304	2.2:+11286723	MS.gene059306:CDS
TCAATGGGTGGCTTCATTTG+AGG	0.460948	2.2:+11287362	MS.gene059306:CDS
AAGGGCATGGGAGGACTTCA+AGG	0.461364	2.2:+11287482	MS.gene059306:CDS
CCGGAGCCAACCGCCTTCAT+GGG	0.476243	2.2:+11288239	MS.gene059306:CDS
GCCACCCATTGATTTCCAAA+AGG	0.480343	2.2:-11287351	None:intergenic
TGTCAACTGCACAAGCAAAA+TGG	0.480824	2.2:-11289336	None:intergenic
GATATGAATATGGCACTTGT+TGG	0.481660	2.2:+11287835	MS.gene059306:CDS
TTTGGAAACATCAGATGGGT+TGG	0.489093	2.2:+11288138	MS.gene059306:intron
CAAAAGGAAAGTCCAACTTC+TGG	0.490074	2.2:+11289242	MS.gene059306:CDS
CATTGTAGTGAGTAATCCTC+CGG	0.490307	2.2:-11288270	None:intergenic
GTGTTTGTTCCTCCATAGAA+AGG	0.493634	2.2:-11287506	None:intergenic
TAACAAGCTAGCTTATTAGC+TGG	0.493726	2.2:-11286315	None:intergenic
TACCAACACATTCATAGCTC+CGG	0.494183	2.2:-11287061	None:intergenic
AATTTGTAGACAAGAGTCTC+AGG	0.495070	2.2:+11287162	MS.gene059306:intron
CCACCCATTGATTTCCAAAA+GGG	0.496575	2.2:-11287350	None:intergenic
AGTCTTTGAAGTAACACAAA+AGG	0.498554	2.2:+11289226	MS.gene059306:intron
ATATCAGAAGATGTACCAAC+TGG	0.500267	2.2:+11288173	MS.gene059306:CDS
AACAATGATAGTCTTTCTGA+TGG	0.507865	2.2:+11286976	MS.gene059306:CDS
TGCAATTAGCAGCTCATAGT+GGG	0.512001	2.2:+11289384	MS.gene059306:CDS
ATATGAATATGGCACTTGTT+GGG	0.515313	2.2:+11287836	MS.gene059306:CDS
TCTTCAGCAGCACCAGAAGT+TGG	0.518813	2.2:-11289254	None:intergenic
AGTGGGAATGGATGTGGACT+AGG	0.519846	2.2:+11289401	MS.gene059306:CDS
GCAGTACATAATAAAGGGCA+TGG	0.521471	2.2:+11287469	MS.gene059306:intron
ATATTATACTTCTTACAGAA+AGG	0.524444	2.2:-11286414	None:intergenic
TATAGCATTTCTTCTATGAA+AGG	0.525259	2.2:-11287528	None:intergenic
TTAGCAGCTCATAGTGGGAA+TGG	0.534161	2.2:+11289389	MS.gene059306:CDS
CTTTGCATATTCAATCCAGT+TGG	0.535136	2.2:-11288188	None:intergenic
GCGCAGCCCATGAAGGCGGT+TGG	0.535181	2.2:-11288245	None:intergenic
GCTCATAGTGGGAATGGATG+TGG	0.536608	2.2:+11289395	MS.gene059306:CDS
ATGATTGGTGGTTCAAGTAC+AGG	0.542267	2.2:-11286252	None:intergenic
GGATTGAATATGCAAAGAAA+AGG	0.542964	2.2:+11288194	MS.gene059306:CDS
TCCGGGAGCGCAGCCCATGA+AGG	0.551643	2.2:-11288252	None:intergenic
ACAGTGTTCAATGTGATGAT+TGG	0.552842	2.2:-11286267	None:intergenic
GTGCAATTAGCAGCTCATAG+TGG	0.552846	2.2:+11289383	MS.gene059306:CDS
TACGAACACAATTACAAAGC+CGG	0.554654	2.2:+11287042	MS.gene059306:CDS
ACACGCGGAAGCTCATTCTC+AGG	0.559771	2.2:-11286204	None:intergenic
ATCCAAACCGTCTTCTTCAA+CGG	0.576571	2.2:+11285663	MS.gene059306:CDS
GTTCAACATAAAAGGCGCAT+TGG	0.577523	2.2:-11287193	None:intergenic
TGAGAATGAGCTTCCGCGTG+TGG	0.580303	2.2:+11286206	MS.gene059306:CDS
TTGTGCCAACCACAAAAGCT+GGG	0.580530	2.2:-11289313	None:intergenic
GCAATCCAAGTATCAGATTG+TGG	0.581850	2.2:+11287813	MS.gene059306:CDS
GTGTTCAATGTGATGATTGG+TGG	0.582750	2.2:-11286264	None:intergenic
TACATAATAAAGGGCATGGG+AGG	0.589666	2.2:+11287473	MS.gene059306:CDS
GCTTTATCAAGAAGAATGGA+AGG	0.597289	2.2:-11287792	None:intergenic
GCTGAAGATGATGCAAATGC+TGG	0.597342	2.2:+11289269	MS.gene059306:CDS
CTAGATATGTAGACTAAGTG+AGG	0.598571	2.2:-11287003	None:intergenic
AGTTGCAGCGACATCAAGAT+CGG	0.601830	2.2:+11285774	MS.gene059306:CDS
TTACATACCGTTGAAGAAGA+CGG	0.605834	2.2:-11285670	None:intergenic
CAGTACATAATAAAGGGCAT+GGG	0.608875	2.2:+11287470	MS.gene059306:intron
CAATGATCCAACAAAAGAGA+TGG	0.612047	2.2:+11288288	MS.gene059306:CDS
CTTCAAGGACCTTTCTATGG+AGG	0.612281	2.2:+11287497	MS.gene059306:CDS
AGCCGGAGCTATGAATGTGT+TGG	0.613715	2.2:+11287059	MS.gene059306:CDS
TATGAATATGGCACTTGTTG+GGG	0.620443	2.2:+11287837	MS.gene059306:CDS
ATTGTAGTGAGTAATCCTCC+GGG	0.635051	2.2:-11288269	None:intergenic
TGCAGTTTATGCTGCAGTGG+CGG	0.642608	2.2:+11285818	MS.gene059306:CDS
TGTTGCAGTTTATGCTGCAG+TGG	0.645716	2.2:+11285815	MS.gene059306:CDS
GGGAGCGCAGCCCATGAAGG+CGG	0.649683	2.2:-11288249	None:intergenic
TTCATGGGCTGCGCTCCCGG+AGG	0.657126	2.2:+11288254	MS.gene059306:CDS
GAGATCAGAATGTTGTACAC+CGG	0.663139	2.2:+11288220	MS.gene059306:CDS
AAGATGATGCAAATGCTGGT+AGG	0.683909	2.2:+11289273	MS.gene059306:CDS
TGTGCCAACCACAAAAGCTG+GGG	0.694857	2.2:-11289312	None:intergenic
GTCACAAACAAATCCACACG+CGG	0.716627	2.2:-11286219	None:intergenic
GTTGAACATAGACTGTGACA+TGG	0.762585	2.2:+11287209	MS.gene059306:CDS
TGCTAAAGAAGACAACAGTG+AGG	0.786213	2.2:-11288319	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAATTAACAAAGTTTAA+TGG	-	chr2.2:11288110-11288129	None:intergenic	15.0%
!!	TAAATAACACTTGAAAATAA+TGG	+	chr2.2:11285958-11285977	MS.gene059306:intron	15.0%
!!!	AAATCTAATTCTTAGTTTTT+TGG	+	chr2.2:11287113-11287132	MS.gene059306:intron	15.0%
!!!	AAATTTTGTTAATGAGAAAA+CGG	+	chr2.2:11287917-11287936	MS.gene059306:intron	15.0%
!!!	AATCTAATTCTTAGTTTTTT+GGG	+	chr2.2:11287114-11287133	MS.gene059306:intron	15.0%
!!!	GTAAAACTATTTTATATTGT+CGG	+	chr2.2:11288077-11288096	MS.gene059306:intron	15.0%
!!	AAATCATTCAAGTATAGTTA+AGG	+	chr2.2:11286723-11286742	MS.gene059306:CDS	20.0%
!!	AACTTTGTTAATTAACTTTG+AGG	+	chr2.2:11288113-11288132	MS.gene059306:intron	20.0%
!!	AAGATATTAATACAGCAATT+TGG	+	chr2.2:11287696-11287715	MS.gene059306:CDS	20.0%
!!	ACTTCAGAAAAATATCTAAA+AGG	-	chr2.2:11286453-11286472	None:intergenic	20.0%
!!	ATATTATACTTCTTACAGAA+AGG	-	chr2.2:11286417-11286436	None:intergenic	20.0%
!!	TAAAATTAACTGCAAAATGT+TGG	-	chr2.2:11287993-11288012	None:intergenic	20.0%
!!	TAATTCCATCATAAAATTGT+TGG	-	chr2.2:11287323-11287342	None:intergenic	20.0%
!!	TACATTCAATAGTTAGTTAA+TGG	-	chr2.2:11286055-11286074	None:intergenic	20.0%
!!	TCAATTAAGAAAGATAAGAA+TGG	-	chr2.2:11287142-11287161	None:intergenic	20.0%
!!	TTTATGCAGTACATAATAAA+GGG	+	chr2.2:11287464-11287483	MS.gene059306:intron	20.0%
!!!	AAGGATTATCATTAAGATTT+TGG	+	chr2.2:11289108-11289127	MS.gene059306:intron	20.0%
!!!	AATTTTGTTAATGAGAAAAC+GGG	+	chr2.2:11287918-11287937	MS.gene059306:intron	20.0%
!!!	ACATGTAATCCTTATTTTTT+GGG	+	chr2.2:11286784-11286803	MS.gene059306:intron	20.0%
!!!	ATCACTTGTTATTAAGATTT+TGG	+	chr2.2:11289358-11289377	MS.gene059306:CDS	20.0%
!!!	CACATTTTTGTATCTTTATT+AGG	+	chr2.2:11287660-11287679	MS.gene059306:intron	20.0%
!!!	CTGAAGATTTTTTAGAAAAT+AGG	+	chr2.2:11288855-11288874	MS.gene059306:intron	20.0%
!!!	GATTTTTTAGAAAATAGGAT+AGG	+	chr2.2:11288860-11288879	MS.gene059306:intron	20.0%
!!!	TACATGTAATCCTTATTTTT+TGG	+	chr2.2:11286783-11286802	MS.gene059306:intron	20.0%
!!!	TATTTTCAAGTGTTATTTAG+TGG	-	chr2.2:11285957-11285976	None:intergenic	20.0%
!!!	TCACTTGTTATTAAGATTTT+GGG	+	chr2.2:11289359-11289378	MS.gene059306:CDS	20.0%
!!!	TGTGTTTTTTAGTATCATAT+AGG	-	chr2.2:11287642-11287661	None:intergenic	20.0%
!!!	TTAATTAACTTTGAGGATTT+TGG	+	chr2.2:11288120-11288139	MS.gene059306:intron	20.0%
!!!	TTAGATATGTCTAGAAATTT+TGG	-	chr2.2:11286859-11286878	None:intergenic	20.0%
!!!	TTAGTTTTGTGAGATATAAT+TGG	+	chr2.2:11288885-11288904	MS.gene059306:intron	20.0%
!!!	TTATGACATAAGAAGTATAA+TGG	+	chr2.2:11286573-11286592	MS.gene059306:intron	20.0%
!	AATTGCATCATCATTAAACT+CGG	-	chr2.2:11288792-11288811	None:intergenic	25.0%
!	ACAAGAATGGTTAAAAATGA+AGG	+	chr2.2:11286503-11286522	MS.gene059306:CDS	25.0%
!	CATAGAAGAAATGCTATATA+TGG	+	chr2.2:11287533-11287552	MS.gene059306:CDS	25.0%
!	CTTTATGCAGTACATAATAA+AGG	+	chr2.2:11287463-11287482	MS.gene059306:intron	25.0%
!	GAAATTCAATATGGAAGAAA+AGG	+	chr2.2:11287569-11287588	MS.gene059306:CDS	25.0%
!	TAATGCAAAAATCTAACCTT+AGG	-	chr2.2:11288500-11288519	None:intergenic	25.0%
!	TAGATCACATAAAGAGTTAA+AGG	-	chr2.2:11289056-11289075	None:intergenic	25.0%
!	TATAGCATTTCTTCTATGAA+AGG	-	chr2.2:11287531-11287550	None:intergenic	25.0%
!	TATCCTGATGAAATTCAATA+TGG	+	chr2.2:11287560-11287579	MS.gene059306:CDS	25.0%
!	TGTAAACACTAAATTGTAAG+AGG	-	chr2.2:11287422-11287441	None:intergenic	25.0%
!	TTTGTAGTTAAGATGCTTAA+TGG	+	chr2.2:11285905-11285924	MS.gene059306:intron	25.0%
!!	AACCATTCTTGTTTAAATTC+TGG	-	chr2.2:11286495-11286514	None:intergenic	25.0%
!!	CAAGTTGAATTAAATATCGA+GGG	+	chr2.2:11288532-11288551	MS.gene059306:intron	25.0%
!!	GACTATTTGTTAAGAGATTA+TGG	+	chr2.2:11288605-11288624	MS.gene059306:intron	25.0%
!!	GATTATCATTAAGATTTTGG+TGG	+	chr2.2:11289111-11289130	MS.gene059306:intron	25.0%
!!	TAGTGTAAAACTAGTTTACA+CGG	-	chr2.2:11287955-11287974	None:intergenic	25.0%
!!	TTGCAGTTAATTTTACATTG+CGG	+	chr2.2:11287998-11288017	MS.gene059306:intron	25.0%
!!	TTTTACTTTCTATGGTCTTT+TGG	+	chr2.2:11286362-11286381	MS.gene059306:CDS	25.0%
!!!	ACTTGTTATTAAGATTTTGG+GGG	+	chr2.2:11289361-11289380	MS.gene059306:CDS	25.0%
!!!	ATTTTGATGGATTCTAAGAA+TGG	+	chr2.2:11287270-11287289	MS.gene059306:CDS	25.0%
!!!	CACTTGTTATTAAGATTTTG+GGG	+	chr2.2:11289360-11289379	MS.gene059306:CDS	25.0%
!!!	CATGTAATCCTTATTTTTTG+GGG	+	chr2.2:11286785-11286804	MS.gene059306:intron	25.0%
!!!	TAGAAATTTTGGTGAAAATG+CGG	-	chr2.2:11286848-11286867	None:intergenic	25.0%
!!!	TGTTCTGTTTTTACTTTCTA+TGG	+	chr2.2:11286354-11286373	MS.gene059306:CDS	25.0%
!!!	TTACAAGAGTATCTATCAAA+AGG	+	chr2.2:11289089-11289108	MS.gene059306:intron	25.0%
!!!	TTCCAAATTTGCTAAGTTTT+GGG	+	chr2.2:11286389-11286408	MS.gene059306:CDS	25.0%
	AACAATGATAGTCTTTCTGA+TGG	+	chr2.2:11286976-11286995	MS.gene059306:CDS	30.0%
	ACATGTATATCAATCATTGC+AGG	-	chr2.2:11286547-11286566	None:intergenic	30.0%
	ATATGAATATGGCACTTGTT+GGG	+	chr2.2:11287836-11287855	MS.gene059306:CDS	30.0%
	ATTGCATCATCATTAAACTC+GGG	-	chr2.2:11288791-11288810	None:intergenic	30.0%
	CACCAGAATTTAAACAAGAA+TGG	+	chr2.2:11286490-11286509	MS.gene059306:CDS	30.0%
	CAGTCTATGTTCAACATAAA+AGG	-	chr2.2:11287204-11287223	None:intergenic	30.0%
	CATAGCATGTTGAATAATCT+TGG	-	chr2.2:11287247-11287266	None:intergenic	30.0%
	CATGTATATCAATCATTGCA+GGG	-	chr2.2:11286546-11286565	None:intergenic	30.0%
	CTTCCATATTGAATTTCATC+AGG	-	chr2.2:11287566-11287585	None:intergenic	30.0%
	CTTTGGTAAAATCCAATTCA+GGG	+	chr2.2:11288367-11288386	MS.gene059306:intron	30.0%
	GATTATGCAATATGCAACTA+GGG	-	chr2.2:11288459-11288478	None:intergenic	30.0%
	GATTGCTTTATCAAGAAGAA+TGG	-	chr2.2:11287799-11287818	None:intergenic	30.0%
	GGATTGAATATGCAAAGAAA+AGG	+	chr2.2:11288194-11288213	MS.gene059306:CDS	30.0%
	TACCCAAAACTTAGCAAATT+TGG	-	chr2.2:11286394-11286413	None:intergenic	30.0%
	TCAACTTCATTCCAATTTGA+GGG	+	chr2.2:11286667-11286686	MS.gene059306:CDS	30.0%
	TCAGATTGTGGATATGAATA+TGG	+	chr2.2:11287825-11287844	MS.gene059306:CDS	30.0%
	TCTTTGGTAAAATCCAATTC+AGG	+	chr2.2:11288366-11288385	MS.gene059306:intron	30.0%
	TGAATATGCAAAGAAAAGGT+TGG	+	chr2.2:11288198-11288217	MS.gene059306:CDS	30.0%
	TGATTATGCAATATGCAACT+AGG	-	chr2.2:11288460-11288479	None:intergenic	30.0%
	TGTATCTAGTAGTATGCTAT+TGG	+	chr2.2:11289441-11289460	MS.gene059306:intron	30.0%
	TTAATTAAGCGCTTATATCC+TGG	-	chr2.2:11288749-11288768	None:intergenic	30.0%
	TTCAACTTCATTCCAATTTG+AGG	+	chr2.2:11286666-11286685	MS.gene059306:CDS	30.0%
	TTGTAATTGTGTTCGTACTT+TGG	-	chr2.2:11287039-11287058	None:intergenic	30.0%
!	AGATGCTTAATGGATATATG+AGG	+	chr2.2:11285915-11285934	MS.gene059306:intron	30.0%
!	GAATTGGATTTTACCAAAGA+GGG	-	chr2.2:11288366-11288385	None:intergenic	30.0%
!	GCAAGTTGAATTAAATATCG+AGG	+	chr2.2:11288531-11288550	MS.gene059306:intron	30.0%
!	GCTTAATGATTTTTGTCATC+TGG	+	chr2.2:11286024-11286043	MS.gene059306:intron	30.0%
!	TGAATTGGATTTTACCAAAG+AGG	-	chr2.2:11288367-11288386	None:intergenic	30.0%
!	TGCTTCCAACAATTTTATGA+TGG	+	chr2.2:11287315-11287334	MS.gene059306:CDS	30.0%
!!	ACGCTTTATGTTAGTCTTAT+TGG	-	chr2.2:11286938-11286957	None:intergenic	30.0%
!!	AGTCTTTGAAGTAACACAAA+AGG	+	chr2.2:11289226-11289245	MS.gene059306:intron	30.0%
!!	GTTTGCGAAAACTTTTTAAG+TGG	-	chr2.2:11286115-11286134	None:intergenic	30.0%
!!	TTATTGTTGGCTTACCAATT+GGG	+	chr2.2:11288400-11288419	MS.gene059306:intron	30.0%
!!!	CTTCCAAATTTGCTAAGTTT+TGG	+	chr2.2:11286388-11286407	MS.gene059306:CDS	30.0%
	AATTTGTAGACAAGAGTCTC+AGG	+	chr2.2:11287162-11287181	MS.gene059306:intron	35.0%
	ACAGTGTTCAATGTGATGAT+TGG	-	chr2.2:11286270-11286289	None:intergenic	35.0%
	ACCACAAGTGTATGGTTTAT+AGG	+	chr2.2:11289161-11289180	MS.gene059306:intron	35.0%
	ATATCAGAAGATGTACCAAC+TGG	+	chr2.2:11288173-11288192	MS.gene059306:CDS	35.0%
	ATTAACTACGAACTCGAAAG+TGG	-	chr2.2:11285987-11286006	None:intergenic	35.0%
	CAAATTCAAGCATGAGTACT+TGG	-	chr2.2:11286909-11286928	None:intergenic	35.0%
	CAATGATCCAACAAAAGAGA+TGG	+	chr2.2:11288288-11288307	MS.gene059306:CDS	35.0%
	CAATTAGAACCACAAGTGTA+TGG	+	chr2.2:11289153-11289172	MS.gene059306:intron	35.0%
	CATATCCACAATCTGATACT+TGG	-	chr2.2:11287821-11287840	None:intergenic	35.0%
	CTAGATATGTAGACTAAGTG+AGG	-	chr2.2:11287006-11287025	None:intergenic	35.0%
	CTTTGCATATTCAATCCAGT+TGG	-	chr2.2:11288191-11288210	None:intergenic	35.0%
	GATATGAATATGGCACTTGT+TGG	+	chr2.2:11287835-11287854	MS.gene059306:CDS	35.0%
	GCAAAATGTTGGATTCTCAT+TGG	-	chr2.2:11287982-11288001	None:intergenic	35.0%
	GCAAACAACCCTTTCAAAAA+TGG	-	chr2.2:11289467-11289486	None:intergenic	35.0%
	GCTTTATCAAGAAGAATGGA+AGG	-	chr2.2:11287795-11287814	None:intergenic	35.0%
	GGGAGTGATAATTCAATTGA+TGG	+	chr2.2:11287762-11287781	MS.gene059306:CDS	35.0%
	GTATTCATATGATATGCACC+AGG	+	chr2.2:11288728-11288747	MS.gene059306:intron	35.0%
	TACGAACACAATTACAAAGC+CGG	+	chr2.2:11287042-11287061	MS.gene059306:CDS	35.0%
	TCAGATCAGAGATATACACA+TGG	-	chr2.2:11288839-11288858	None:intergenic	35.0%
	TCCTATAAACCATACACTTG+TGG	-	chr2.2:11289165-11289184	None:intergenic	35.0%
	TCTAACCTTAGGAAAGATAC+TGG	-	chr2.2:11288489-11288508	None:intergenic	35.0%
	TTACATACCGTTGAAGAAGA+CGG	-	chr2.2:11285673-11285692	None:intergenic	35.0%
	TTCTGGTGAATCTTCACTAT+TGG	-	chr2.2:11286478-11286497	None:intergenic	35.0%
!	ACATGCTATGTGCATTTTGA+TGG	+	chr2.2:11287257-11287276	MS.gene059306:CDS	35.0%
!	CAGTAACACATGCTTTTGAA+GGG	+	chr2.2:11287742-11287761	MS.gene059306:CDS	35.0%
!	CAGTACATAATAAAGGGCAT+GGG	+	chr2.2:11287470-11287489	MS.gene059306:intron	35.0%
!	GGAATTAAAGATGACCCTTT+TGG	+	chr2.2:11287336-11287355	MS.gene059306:CDS	35.0%
!	TAACAAGCTAGCTTATTAGC+TGG	-	chr2.2:11286318-11286337	None:intergenic	35.0%
!	TATGAATATGGCACTTGTTG+GGG	+	chr2.2:11287837-11287856	MS.gene059306:CDS	35.0%
!	TCAGTAACACATGCTTTTGA+AGG	+	chr2.2:11287741-11287760	MS.gene059306:CDS	35.0%
!	TCTTGCTAATTAGTCTCTCA+AGG	+	chr2.2:11286141-11286160	MS.gene059306:intron	35.0%
!	TTGAAAGGGTTGTTTGCTAA+GGG	+	chr2.2:11289470-11289489	MS.gene059306:intron	35.0%
!	TTTGAAAGGGTTGTTTGCTA+AGG	+	chr2.2:11289469-11289488	MS.gene059306:intron	35.0%
!!	AAAGCGTTGACAGGTTATAT+TGG	+	chr2.2:11286951-11286970	MS.gene059306:intron	35.0%
!!	AAGACGGTTTGGATATGTTT+TGG	-	chr2.2:11285657-11285676	None:intergenic	35.0%
!!	AGGATTTTGGAAACATCAGA+TGG	+	chr2.2:11288133-11288152	MS.gene059306:intron	35.0%
!!	ATGACCCTTTTGGAAATCAA+TGG	+	chr2.2:11287346-11287365	MS.gene059306:CDS	35.0%
!!	CTTATTGTTGGCTTACCAAT+TGG	+	chr2.2:11288399-11288418	MS.gene059306:intron	35.0%
!!	CTTTTCACTGTCTTATTCCA+GGG	+	chr2.2:11289014-11289033	MS.gene059306:intron	35.0%
!!	GCTTGTTATGTTTCTGATGA+TGG	+	chr2.2:11286330-11286349	MS.gene059306:CDS	35.0%
!!	GGATTTTGGAAACATCAGAT+GGG	+	chr2.2:11288134-11288153	MS.gene059306:intron	35.0%
!!	TATTGTTGGCTTACCAATTG+GGG	+	chr2.2:11288401-11288420	MS.gene059306:intron	35.0%
!!	TGACCCTTTTGGAAATCAAT+GGG	+	chr2.2:11287347-11287366	MS.gene059306:CDS	35.0%
!!	TTGTCTTTTGTGTTTGTTGC+AGG	+	chr2.2:11286597-11286616	MS.gene059306:intron	35.0%
!!!	GCGAAAACTTTTTAAGTGGT+TGG	-	chr2.2:11286111-11286130	None:intergenic	35.0%
!!!	GCTATTGGCCATTTTTGAAA+GGG	+	chr2.2:11289456-11289475	MS.gene059306:intron	35.0%
!!!	GGTTTTTTGAATCAGCGTAT+TGG	+	chr2.2:11288553-11288572	MS.gene059306:intron	35.0%
!!!	GTAATCCTTATTTTTTGGGG+AGG	+	chr2.2:11286788-11286807	MS.gene059306:intron	35.0%
!!!	TGCTATTGGCCATTTTTGAA+AGG	+	chr2.2:11289455-11289474	MS.gene059306:intron	35.0%
	AAGATGATGCAAATGCTGGT+AGG	+	chr2.2:11289273-11289292	MS.gene059306:CDS	40.0%
	AGCAAGCATTCTCCTGTTAT+AGG	+	chr2.2:11288338-11288357	MS.gene059306:CDS	40.0%
	ATCCAAACCGTCTTCTTCAA+CGG	+	chr2.2:11285663-11285682	MS.gene059306:CDS	40.0%
	ATTGTAGTGAGTAATCCTCC+GGG	-	chr2.2:11288272-11288291	None:intergenic	40.0%
	CAAAAGGAAAGTCCAACTTC+TGG	+	chr2.2:11289242-11289261	MS.gene059306:CDS	40.0%
	CAACAAAAGAGATGGTCTTC+AGG	+	chr2.2:11288296-11288315	MS.gene059306:CDS	40.0%
	CAACACCAGTATCTTTCCTA+AGG	+	chr2.2:11288481-11288500	MS.gene059306:intron	40.0%
	CATTGTAGTGAGTAATCCTC+CGG	-	chr2.2:11288273-11288292	None:intergenic	40.0%
	CCTGGAATAAGACAGTGAAA+AGG	-	chr2.2:11289016-11289035	None:intergenic	40.0%
	CTGCATATTCTCCCTCAAAT+TGG	-	chr2.2:11286681-11286700	None:intergenic	40.0%
	CTTAGGAAAGATACTGGTGT+TGG	-	chr2.2:11288483-11288502	None:intergenic	40.0%
	GAAATCAGTGCAAGACTTCA+TGG	-	chr2.2:11285856-11285875	None:intergenic	40.0%
	GAGATCAGAATGTTGTACAC+CGG	+	chr2.2:11288220-11288239	MS.gene059306:CDS	40.0%
	GCAATCCAAGTATCAGATTG+TGG	+	chr2.2:11287813-11287832	MS.gene059306:CDS	40.0%
	GCAGTACATAATAAAGGGCA+TGG	+	chr2.2:11287469-11287488	MS.gene059306:intron	40.0%
	GGTTATAAAGTTGCCTTGTG+TGG	+	chr2.2:11288019-11288038	MS.gene059306:intron	40.0%
	GTCAACCTCCCCAAAAAATA+AGG	-	chr2.2:11286796-11286815	None:intergenic	40.0%
	GTGTTCAATGTGATGATTGG+TGG	-	chr2.2:11286267-11286286	None:intergenic	40.0%
	GTGTTTGTTCCTCCATAGAA+AGG	-	chr2.2:11287509-11287528	None:intergenic	40.0%
	GTTGAACATAGACTGTGACA+TGG	+	chr2.2:11287209-11287228	MS.gene059306:CDS	40.0%
	TACCAACACATTCATAGCTC+CGG	-	chr2.2:11287064-11287083	None:intergenic	40.0%
	TGCAATTAGCAGCTCATAGT+GGG	+	chr2.2:11289384-11289403	MS.gene059306:CDS	40.0%
	TGCTAAAGAAGACAACAGTG+AGG	-	chr2.2:11288322-11288341	None:intergenic	40.0%
	TGTCAACTGCACAAGCAAAA+TGG	-	chr2.2:11289339-11289358	None:intergenic	40.0%
	TTTGGAAACATCAGATGGGT+TGG	+	chr2.2:11288138-11288157	MS.gene059306:intron	40.0%
!	ATGATTGGTGGTTCAAGTAC+AGG	-	chr2.2:11286255-11286274	None:intergenic	40.0%
!	CTGAAGACCATCTCTTTTGT+TGG	-	chr2.2:11288298-11288317	None:intergenic	40.0%
!	GAACACTGTTTTGTCTCTGT+TGG	+	chr2.2:11286281-11286300	MS.gene059306:CDS	40.0%
!	GCGTCACATCTTCAATTTTG+TGG	-	chr2.2:11286642-11286661	None:intergenic	40.0%
!	GTTCAACATAAAAGGCGCAT+TGG	-	chr2.2:11287196-11287215	None:intergenic	40.0%
!	TACATAATAAAGGGCATGGG+AGG	+	chr2.2:11287473-11287492	MS.gene059306:CDS	40.0%
!!	AGTTTGCTGTCGTTAGATGT+CGG	+	chr2.2:11288053-11288072	MS.gene059306:intron	40.0%
!!	CATCAGATGGGTTGGTTATA+TGG	+	chr2.2:11288146-11288165	MS.gene059306:intron	40.0%
!!	CCACCCATTGATTTCCAAAA+GGG	-	chr2.2:11287353-11287372	None:intergenic	40.0%
!!	CCTTTTCACTGTCTTATTCC+AGG	+	chr2.2:11289013-11289032	MS.gene059306:intron	40.0%
!!	GACTAACATAAAGCGTTGAC+AGG	+	chr2.2:11286942-11286961	MS.gene059306:intron	40.0%
!!!	GGATATGTTTTGGTAACAGC+AGG	-	chr2.2:11285647-11285666	None:intergenic	40.0%
	AGTGCCATGCTTAAGCTCTT+GGG	+	chr2.2:11289191-11289210	MS.gene059306:intron	45.0%
	AGTTGCAGCGACATCAAGAT+CGG	+	chr2.2:11285774-11285793	MS.gene059306:CDS	45.0%
	CATTAAACTCGGGCTCTGTT+TGG	-	chr2.2:11288781-11288800	None:intergenic	45.0%
	CCTGTTATAGGTACCCTCTT+TGG	+	chr2.2:11288350-11288369	MS.gene059306:intron	45.0%
	CGTTCATTGCTAGTCGATGT+AGG	+	chr2.2:11286882-11286901	MS.gene059306:intron	45.0%
	CTTCAAAGACAGACCACACA+AGG	-	chr2.2:11288035-11288054	None:intergenic	45.0%
	CTTCAAGGACCTTTCTATGG+AGG	+	chr2.2:11287497-11287516	MS.gene059306:CDS	45.0%
	GCGTATTGGAGCTTATCTAC+TGG	+	chr2.2:11288567-11288586	MS.gene059306:intron	45.0%
	GCTGAAGATGATGCAAATGC+TGG	+	chr2.2:11289269-11289288	MS.gene059306:CDS	45.0%
	GGACTTCAAGGACCTTTCTA+TGG	+	chr2.2:11287494-11287513	MS.gene059306:CDS	45.0%
	GGTAAAATCCAATTCAGGGC+AGG	+	chr2.2:11288371-11288390	MS.gene059306:intron	45.0%
	GTCACAAACAAATCCACACG+CGG	-	chr2.2:11286222-11286241	None:intergenic	45.0%
	GTGCAATTAGCAGCTCATAG+TGG	+	chr2.2:11289383-11289402	MS.gene059306:CDS	45.0%
	TACCGTTGAAGAAGACGGTT+TGG	-	chr2.2:11285668-11285687	None:intergenic	45.0%
	TATCCCCAAGAGCTTAAGCA+TGG	-	chr2.2:11289198-11289217	None:intergenic	45.0%
	TCAATGGGTGGCTTCATTTG+AGG	+	chr2.2:11287362-11287381	MS.gene059306:CDS	45.0%
	TCATTCTCAGGTACTCTAGC+AGG	-	chr2.2:11286195-11286214	None:intergenic	45.0%
	TGTTGCAGTTTATGCTGCAG+TGG	+	chr2.2:11285815-11285834	MS.gene059306:CDS	45.0%
	TTGTGCCAACCACAAAAGCT+GGG	-	chr2.2:11289316-11289335	None:intergenic	45.0%
!	ACTGTCTTATTCCAGGGACT+TGG	+	chr2.2:11289020-11289039	MS.gene059306:intron	45.0%
!	CTGTCTTATTCCAGGGACTT+GGG	+	chr2.2:11289021-11289040	MS.gene059306:intron	45.0%
!	TTAGCAGCTCATAGTGGGAA+TGG	+	chr2.2:11289389-11289408	MS.gene059306:CDS	45.0%
!!	CCAAAGAGGGTACCTATAAC+AGG	-	chr2.2:11288353-11288372	None:intergenic	45.0%
!!	CCCTTTTGGAAATCAATGGG+TGG	+	chr2.2:11287350-11287369	MS.gene059306:CDS	45.0%
!!	GCCACCCATTGATTTCCAAA+AGG	-	chr2.2:11287354-11287373	None:intergenic	45.0%
	AAGACAAGCCTGCCCTGAAT+TGG	-	chr2.2:11288382-11288401	None:intergenic	50.0%
	AGCCGGAGCTATGAATGTGT+TGG	+	chr2.2:11287059-11287078	MS.gene059306:CDS	50.0%
	AGTGGGAATGGATGTGGACT+AGG	+	chr2.2:11289401-11289420	MS.gene059306:CDS	50.0%
	GAGTGCCATGCTTAAGCTCT+TGG	+	chr2.2:11289190-11289209	MS.gene059306:intron	50.0%
	GTGCCATGCTTAAGCTCTTG+GGG	+	chr2.2:11289192-11289211	MS.gene059306:intron	50.0%
	GTTGTGCCAACCACAAAAGC+TGG	-	chr2.2:11289317-11289336	None:intergenic	50.0%
	TACCAATTGGGGTCTGCGTT+CGG	+	chr2.2:11288412-11288431	MS.gene059306:intron	50.0%
	TATTCCAGGGACTTGGGCTA+TGG	+	chr2.2:11289027-11289046	MS.gene059306:intron	50.0%
	TCTTCAGCAGCACCAGAAGT+TGG	-	chr2.2:11289257-11289276	None:intergenic	50.0%
	TGCAGTTTATGCTGCAGTGG+CGG	+	chr2.2:11285818-11285837	MS.gene059306:CDS	50.0%
	TGTGACCGACAAGAAAGACC+AGG	+	chr2.2:11286819-11286838	MS.gene059306:intron	50.0%
	TGTGCCAACCACAAAAGCTG+GGG	-	chr2.2:11289315-11289334	None:intergenic	50.0%
	TTGGTGAAAATGCGGTGTCC+TGG	-	chr2.2:11286840-11286859	None:intergenic	50.0%
!	GCTCATAGTGGGAATGGATG+TGG	+	chr2.2:11289395-11289414	MS.gene059306:CDS	50.0%
!	GGGCAGGCTTGTCTTATTGT+TGG	+	chr2.2:11288387-11288406	MS.gene059306:intron	50.0%
!	GGTGTCCTGGTCTTTCTTGT+CGG	-	chr2.2:11286827-11286846	None:intergenic	50.0%
!	TGATGAGTCCCCAGCTTTTG+TGG	+	chr2.2:11289304-11289323	MS.gene059306:CDS	50.0%
!	TGGCACTTGTTGGGGTAAAC+AGG	+	chr2.2:11287845-11287864	MS.gene059306:CDS	50.0%
	ACACGCGGAAGCTCATTCTC+AGG	-	chr2.2:11286207-11286226	None:intergenic	55.0%
	GAATCCATAGCCCAAGTCCC+TGG	-	chr2.2:11289034-11289053	None:intergenic	55.0%
	TGAGAATGAGCTTCCGCGTG+TGG	+	chr2.2:11286206-11286225	MS.gene059306:CDS	55.0%
!	AAGGGCATGGGAGGACTTCA+AGG	+	chr2.2:11287482-11287501	MS.gene059306:CDS	55.0%
!	GAGTCCCCAGCTTTTGTGGT+TGG	+	chr2.2:11289308-11289327	MS.gene059306:CDS	55.0%
	GACCGAACGCAGACCCCAAT+TGG	-	chr2.2:11288417-11288436	None:intergenic	60.0%
	ACCGGAGCCAACCGCCTTCA+TGG	+	chr2.2:11288238-11288257	MS.gene059306:CDS	65.0%
	CCGGAGCCAACCGCCTTCAT+GGG	+	chr2.2:11288239-11288258	MS.gene059306:CDS	65.0%
!	CCCATGAAGGCGGTTGGCTC+CGG	-	chr2.2:11288242-11288261	None:intergenic	65.0%
	GCGCAGCCCATGAAGGCGGT+TGG	-	chr2.2:11288248-11288267	None:intergenic	70.0%
!	GCCTTCATGGGCTGCGCTCC+CGG	+	chr2.2:11288251-11288270	MS.gene059306:CDS	70.0%
!	GGGAGCGCAGCCCATGAAGG+CGG	-	chr2.2:11288252-11288271	None:intergenic	70.0%
!	TCCGGGAGCGCAGCCCATGA+AGG	-	chr2.2:11288255-11288274	None:intergenic	70.0%
!	TTCATGGGCTGCGCTCCCGG+AGG	+	chr2.2:11288254-11288273	MS.gene059306:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	11285637	11289495	11285637	ID=MS.gene059306
chr2.2	mRNA	11285637	11289495	11285637	ID=MS.gene059306.t1;Parent=MS.gene059306
chr2.2	exon	11285637	11285684	11285637	ID=MS.gene059306.t1.exon1;Parent=MS.gene059306.t1
chr2.2	CDS	11285637	11285684	11285637	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11285774	11285859	11285774	ID=MS.gene059306.t1.exon2;Parent=MS.gene059306.t1
chr2.2	CDS	11285774	11285859	11285774	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11286200	11286524	11286200	ID=MS.gene059306.t1.exon3;Parent=MS.gene059306.t1
chr2.2	CDS	11286200	11286524	11286200	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11286619	11286744	11286619	ID=MS.gene059306.t1.exon4;Parent=MS.gene059306.t1
chr2.2	CDS	11286619	11286744	11286619	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11286964	11287080	11286964	ID=MS.gene059306.t1.exon5;Parent=MS.gene059306.t1
chr2.2	CDS	11286964	11287080	11286964	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11287171	11287383	11287171	ID=MS.gene059306.t1.exon6;Parent=MS.gene059306.t1
chr2.2	CDS	11287171	11287383	11287171	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11287473	11287590	11287473	ID=MS.gene059306.t1.exon7;Parent=MS.gene059306.t1
chr2.2	CDS	11287473	11287590	11287473	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11287682	11287866	11287682	ID=MS.gene059306.t1.exon8;Parent=MS.gene059306.t1
chr2.2	CDS	11287682	11287866	11287682	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11288152	11288359	11288152	ID=MS.gene059306.t1.exon9;Parent=MS.gene059306.t1
chr2.2	CDS	11288152	11288359	11288152	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11289228	11289422	11289228	ID=MS.gene059306.t1.exon10;Parent=MS.gene059306.t1
chr2.2	CDS	11289228	11289422	11289228	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1
chr2.2	exon	11289491	11289495	11289491	ID=MS.gene059306.t1.exon11;Parent=MS.gene059306.t1
chr2.2	CDS	11289491	11289495	11289491	ID=cds.MS.gene059306.t1;Parent=MS.gene059306.t1

Gene Sequence

>MS.gene059306

ATGGAGTCCTGCTGTTACCAAAACATATCCAAACCGTCTTCTTCAACGGTATGTAATATAAACTGTGTCGCAATATTTGATATTCTTGTAAGTTACTGTCTTTACTCTGTGTATGTAATTGATGCAACTCAATCAAGAGTTGCAGCGACATCAAGATCGGTATCAAAATTAATCTTGATGTTGCAGTTTATGCTGCAGTGGCGGTTAACAACATTTCCATGAAGTCTTGCACTGATTTCGTTACAACTTGATAAGTGTTCAGTTCAAGTTTGTAGTTAAGATGCTTAATGGATATATGAGGTGTCAAAATTGATTAACCACTAAATAACACTTGAAAATAATGGTTACCACTTTCGAGTTCGTAGTTAATGAAATTATCTTGAAGATGCTTAATGATTTTTGTCATCTGGTATATCCATTAACTAACTATTGAATGTAATTAACTACTTATTTTAACTTATTTAAACTATACCAACCACTTAAAAAGTTTTCGCAAACTATTTCTCTTGCTAATTAGTCTCTCAAGGTTTGTGCTTATTTTGTTTTTTTTTTTTTCCTGCTAGAGTACCTGAGAATGAGCTTCCGCGTGTGGATTTGTTTGTGACAACAGCAGATCCTGTACTTGAACCACCAATCATCACATTGAACACTGTTTTGTCTCTGTTGGCTCTTGATTATCCAGCTAATAAGCTAGCTTGTTATGTTTCTGATGATGGCTGTTCTGTTTTTACTTTCTATGGTCTTTTGGAAGCTTCCAAATTTGCTAAGTTTTGGGTACCTTTCTGTAAGAAGTATAATATACAAGTTAGAGCACCTTTTAGATATTTTTCTGAAGTTACCAATAGTGAAGATTCACCAGAATTTAAACAAGAATGGTTAAAAATGAAGGTAAGTAGTATAATATTACCCTGCAATGATTGATATACATGTTATGAGTTATGACATAAGAAGTATAATGGTTTGTCTTTTGTGTTTGTTGCAGGATATGTATGACAATCTTAGCCACAAAATTGAAGATGTGACGCTAAATTCAACTTCATTCCAATTTGAGGGAGAATATGCAGTGTTCTTAAATACAGAAAAAAGAAATCATTCAAGTATAGTTAAGGTAACTAATATAGTAAAAAATATTCGTGCATCTATAACTACATGTAATCCTTATTTTTTGGGGAGGTTGACTGTTGTGACCGACAAGAAAGACCAGGACACCGCATTTTCACCAAAATTTCTAGACATATCTAAGTTCGTTCATTGCTAGTCGATGTAGGACCAAGTACTCATGCTTGAATTTGAGCAAACCAATAAGACTAACATAAAGCGTTGACAGGTTATATTGGAGAACAATGATAGTCTTTCTGATGGATTGCCTCACTTAGTCTACATATCTAGAGAGAAGAGACCAAAGTACGAACACAATTACAAAGCCGGAGCTATGAATGTGTTGGTAATTTTTCAGACTAATATTTAGTGTTGTGAAAATCTAATTCTTAGTTTTTTGGGAACCATTCTTATCTTTCTTAATTGAAATTTGTAGACAAGAGTCTCAGGATTGATGACCAATGCGCCTTTTATGTTGAACATAGACTGTGACATGGTTGTGAACAATGCCAAGATTATTCAACATGCTATGTGCATTTTGATGGATTCTAAGAATGGAAAAGATGTTGCTTTTGTTCAATGCTTCCAACAATTTTATGATGGAATTAAAGATGACCCTTTTGGAAATCAATGGGTGGCTTCATTTGAGGTTTCTTCACATCTTCAAATGCATCTATCGCTTTACCTCTTACAATTTAGTGTTTACAAATTTTTTATGTTTTGTTTTACTTTATGCAGTACATAATAAAGGGCATGGGAGGACTTCAAGGACCTTTCTATGGAGGAACAAACACCTTTCATAGAAGAAATGCTATATATGGTCTTTATCCTGATGAAATTCAATATGGAAGAAAAGGTCACTTATTCATTGCATATCTTAGTGTGTTACTATTAATAGTTGATTCCTATATGATACTAAAAAACACACATTTTTGTATCTTTATTAGGAAAAATAACAGAAAAGATATTAATACAGCAATTTGGAAGCTCAAGAGAGTTTGTCAAATCAGTAACACATGCTTTTGAAGGGAGTGATAATTCAATTGATGGTATTAGTCCTTCCATTCTTCTTGATAAAGCAATCCAAGTATCAGATTGTGGATATGAATATGGCACTTGTTGGGGTAAACAGGTAAGTTACATAAACAAATTTATTTTTCACACAAACTATTTTCAAAATTTAAATTTTGTTAATGAGAAAACGGGACATGCACAGTCCGTGTAAACTAGTTTTACACTAACGTCCAATGAGAATCCAACATTTTGCAGTTAATTTTACATTGCGGTTATAAAGTTGCCTTGTGTGGTCTGTCTTTGAAGTTTGCTGTCGTTAGATGTCGGTGTAAAACTATTTTATATTGTCGGTGCATCTCCATTAAACTTTGTTAATTAACTTTGAGGATTTTGGAAACATCAGATGGGTTGGTTATATGGATCAATATCAGAAGATGTACCAACTGGATTGAATATGCAAAGAAAAGGTTGGAGATCAGAATGTTGTACACCGGAGCCAACCGCCTTCATGGGCTGCGCTCCCGGAGGATTACTCACTACAATGATCCAACAAAAGAGATGGTCTTCAGGCCTCACTGTTGTCTTCTTTAGCAAGCATTCTCCTGTTATAGGTACCCTCTTTGGTAAAATCCAATTCAGGGCAGGCTTGTCTTATTGTTGGCTTACCAATTGGGGTCTGCGTTCGGTCTTTGAAGTTTCTTATGCAGCCCTAGTTGCATATTGCATAATCACCAACACCAGTATCTTTCCTAAGGTTAGATTTTTGCATTAAATTTCTAGTAGCAAGTTGAATTAAATATCGAGGGTTTTTTGAATCAGCGTATTGGAGCTTATCTACTGGCATACGTAAGTGTGAGACTATTTGTTAAGAGATTATGGAAAAAAAACATAAGACATGTTTACAAGTTCTTTTCAAGTTATTTCTATAAGTTTTCAGTTTGACTTTATTTTATTTTATATAGAAATAGTTTATACATAAGTATTCATATGATATGCACCAGGATATAAGCGCTTAATTAAATTGTTTCTCCAAACAGAGCCCGAGTTTAATGATGATGCAATTAGTGTAAAAAAAAAAAAAAAACAACCATGTGTATATCTCTGATCTGAAGATTTTTTAGAAAATAGGATAGGTGTTAGTTTTGTGAGATATAATTGGATGCACATGTAAAACTGTGTGTTGTACGTTAATGTTAAACTCTTAAATGTAAGTACTATATAAGATTTCTTTAATTTTGAGTGTAATATACTACTTTTTTGTTCACCTTTTCACTGTCTTATTCCAGGGACTTGGGCTATGGATTCCTTTAACTCTTTATGTGATCTATACTATACATACATTACAAGAGTATCTATCAAAAGGATTATCATTAAGATTTTGGTGGAACAATCAGAGAATGATAACAATTAGAACCACAAGTGTATGGTTTATAGGATTTTTGAGTGCCATGCTTAAGCTCTTGGGGATATCTGATACAGTCTTTGAAGTAACACAAAAGGAAAGTCCAACTTCTGGTGCTGCTGAAGATGATGCAAATGCTGGTAGGTTCACATTTGATGAGTCCCCAGCTTTTGTGGTTGGCACAACCATTTTGCTTGTGCAGTTGACATCACTTGTTATTAAGATTTTGGGGGTGCAATTAGCAGCTCATAGTGGGAATGGATGTGGACTAGGTGAGTTAATGTGTAGTGTGTATCTAGTAGTATGCTATTGGCCATTTTTGAAAGGGTTGTTTGCTAAGGGTAA

Protein sequence

>MS.gene059306.t1

MESCCYQNISKPSSSTSCSDIKIGIKINLDVAVYAAVAVNNISMKVPENELPRVDLFVTTADPVLEPPIITLNTVLSLLALDYPANKLACYVSDDGCSVFTFYGLLEASKFAKFWVPFCKKYNIQVRAPFRYFSEVTNSEDSPEFKQEWLKMKDMYDNLSHKIEDVTLNSTSFQFEGEYAVFLNTEKRNHSSIVKVILENNDSLSDGLPHLVYISREKRPKYEHNYKAGAMNVLTRVSGLMTNAPFMLNIDCDMVVNNAKIIQHAMCILMDSKNGKDVAFVQCFQQFYDGIKDDPFGNQWVASFEYIIKGMGGLQGPFYGGTNTFHRRNAIYGLYPDEIQYGRKGKITEKILIQQFGSSREFVKSVTHAFEGSDNSIDGISPSILLDKAIQVSDCGYEYGTCWGKQMGWLYGSISEDVPTGLNMQRKGWRSECCTPEPTAFMGCAPGGLLTTMIQQKRWSSGLTVVFFSKHSPVIVFEVTQKESPTSGAAEDDANAGRFTFDESPAFVVGTTILLVQLTSLVIKILGVQLAAHSGNGCGLG