AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059414

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059414.t1 MTR_6g047800 96.764 309 9 1 1 308 1 309 0.0 613
MS.gene059414.t1 MTR_6g047800 96.667 300 9 1 10 308 1 300 0.0 596
MS.gene059414.t1 MTR_2g014590 78.355 231 50 0 1 231 2 232 1.47e-137 389
MS.gene059414.t1 MTR_2g014570 69.492 59 15 2 249 307 7 62 8.17e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059414.t1 AT5G14600 74.286 315 74 2 1 308 1 315 2.68e-175 488

Find 70 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 6.3:+42210263 MS.gene059414:CDS
TAGCTCCTACTCCTGAATTA+TGG 0.261887 6.3:+42210193 MS.gene059414:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 6.3:+42210014 MS.gene059414:CDS
TCCCAATTCTTCTTCTGTTA+TGG 0.281081 6.3:+42212275 MS.gene059414:CDS
GGTTGCGTCGTTCTTGAATC+TGG 0.281471 6.3:+42210297 MS.gene059414:CDS
TCCTGAAGAATTTGCTGGCT+TGG 0.291582 6.3:+42211856 MS.gene059414:CDS
GTCTAAAGTATTCAACAATA+AGG 0.308450 6.3:+42210152 MS.gene059414:CDS
TTGTTTATGTGAAAGATATA+AGG 0.311677 6.3:+42212112 MS.gene059414:intron
GATTGGATAGGAAAGTCATT+TGG 0.331486 6.3:+42210129 MS.gene059414:CDS
AAAACTAAAGTCCATAATTC+AGG 0.343342 6.3:-42210204 None:intergenic
CTTGAATCTGGCACTGGAAG+TGG 0.346516 6.3:+42210309 MS.gene059414:CDS
GCCAAGCCAGCAAATTCTTC+AGG 0.357310 6.3:-42211857 None:intergenic
AGCCATAACAGAAGAAGAAT+TGG 0.358884 6.3:-42212277 None:intergenic
GGGTTTCCTGAAGAATTTGC+TGG 0.381718 6.3:+42211851 MS.gene059414:CDS
ATGTATTTGGAGATTGTTCC+TGG 0.385811 6.3:+42210276 MS.gene059414:CDS
AGGGATGACTTTGAGAAGAT+TGG 0.392112 6.3:+42211782 MS.gene059414:intron
GACGGTAATGACGGCTCTAA+TGG 0.408113 6.3:+42212198 MS.gene059414:CDS
GATCCCTCAGATACCGTTAC+TGG 0.416695 6.3:-42210069 None:intergenic
CGGACATACGAAGTTCTAGA+AGG 0.420477 6.3:+42212153 MS.gene059414:CDS
ATTGGATAGGAAAGTCATTT+GGG 0.422230 6.3:+42210130 MS.gene059414:CDS
TCTAAAGTATTCAACAATAA+GGG 0.468151 6.3:+42210153 MS.gene059414:CDS
TCGGGAAGTACCGGTGAAGT+TGG 0.488752 6.3:-42212017 None:intergenic
TTGAATCTGGCACTGGAAGT+GGG 0.495210 6.3:+42210310 MS.gene059414:CDS
GCCATAACAGAAGAAGAATT+GGG 0.501842 6.3:-42212276 None:intergenic
CTTTAACCACTTCACTTGCA+AGG 0.505869 6.3:+42210334 MS.gene059414:CDS
ATTTGGCAATTGTTTATGAA+AGG 0.507354 6.3:+42210031 MS.gene059414:CDS
CGAAGTTCTAGAAGGGAAAG+TGG 0.510165 6.3:+42212161 MS.gene059414:CDS
TTTAAACATTCAGATTGGAT+AGG 0.513874 6.3:+42210117 MS.gene059414:CDS
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 6.3:+42210351 MS.gene059414:CDS
GTGGAAAGCTCTGAGATTGA+CGG 0.522062 6.3:+42212180 MS.gene059414:CDS
GTCGTTCTTGAATCTGGCAC+TGG 0.536486 6.3:+42210303 MS.gene059414:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 6.3:-42211974 None:intergenic
AAAGTCCATAATTCAGGAGT+AGG 0.545264 6.3:-42210198 None:intergenic
ATAAGCAGCTTAGTCACTGC+GGG 0.545723 6.3:+42211806 MS.gene059414:CDS
GCGGGAGTGAGGGACATTCA+AGG 0.551907 6.3:+42211824 MS.gene059414:CDS
GCTTAGTCACTGCGGGAGTG+AGG 0.551945 6.3:+42211813 MS.gene059414:CDS
GGAATAGCGAGCCAAGGTTG+AGG 0.553281 6.3:-42211902 None:intergenic
GAAGCCAGTAACGGTATCTG+AGG 0.567229 6.3:+42210065 MS.gene059414:CDS
GAACGTTGCCTCCTCTTGCA+AGG 0.567462 6.3:-42212228 None:intergenic
CTTAGTCACTGCGGGAGTGA+GGG 0.567587 6.3:+42211814 MS.gene059414:CDS
GATTCAAGAACGACGCAACC+AGG 0.571390 6.3:-42210294 None:intergenic
AACTGCCCTTGCAAGTGAAG+TGG 0.571466 6.3:-42210340 None:intergenic
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 6.3:+42212217 MS.gene059414:CDS
TTTAACCACTTCACTTGCAA+GGG 0.578833 6.3:+42210335 MS.gene059414:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.582946 6.3:+42211830 MS.gene059414:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 6.3:+42212007 MS.gene059414:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 6.3:+42212220 MS.gene059414:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 6.3:-42211973 None:intergenic
TGACCTCTAGCTTCACCGCA+TGG 0.587593 6.3:-42212303 None:intergenic
TCTGAGATTGACGGTAATGA+CGG 0.588128 6.3:+42212189 MS.gene059414:CDS
AGTAACGGTATCTGAGGGAT+CGG 0.588437 6.3:+42210071 MS.gene059414:CDS
TTTAATAGAACAATTAGTAA+CGG 0.596180 6.3:+42210006 MS.gene059414:CDS
CTAGAACTTCGTATGTCCGT+AGG 0.597219 6.3:-42212149 None:intergenic
AAGAGGAGGCAACGTTCAGA+TGG 0.606722 6.3:+42212234 MS.gene059414:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 6.3:-42210366 None:intergenic
GGACATACGAAGTTCTAGAA+GGG 0.618904 6.3:+42212154 MS.gene059414:CDS
ACGTTCAGATGGAGCTAGCG+TGG 0.623569 6.3:+42212245 MS.gene059414:CDS
TGAGGGACATTCAAGGTGAG+GGG 0.627956 6.3:+42211831 MS.gene059414:CDS
AACAAAGATCTGGATCAGCT+AGG 0.636973 6.3:+42210400 MS.gene059414:CDS
AGACCATGCGGTGAAGCTAG+AGG 0.638675 6.3:+42212300 MS.gene059414:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.642071 6.3:+42211829 MS.gene059414:CDS
AAAGTATTCAACAATAAGGG+CGG 0.646223 6.3:+42210156 MS.gene059414:CDS
TATATAGAATCTGTGTCCTG+TGG 0.646737 6.3:-42210230 None:intergenic
GCTGAAGGAATAGCGAGCCA+AGG 0.650147 6.3:-42211908 None:intergenic
AAGCCAGTAACGGTATCTGA+GGG 0.657172 6.3:+42210066 MS.gene059414:CDS
TCTGTTATGGCTAGACCATG+CGG 0.664012 6.3:+42212288 MS.gene059414:CDS
TGACAACATGAAGCCAGTAA+CGG 0.669534 6.3:+42210056 MS.gene059414:CDS
CATAAGCAGCTTAGTCACTG+CGG 0.689323 6.3:+42211805 MS.gene059414:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.703760 6.3:+42212309 MS.gene059414:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 6.3:-42211972 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTCAATTATATATAAAACA+AGG - chr6.3:42211477-42211496 None:intergenic 15.0%
!! GTAACTTATATATTGTATTA+CGG - chr6.3:42211416-42211435 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + chr6.3:42210006-42210025 MS.gene059414:CDS 15.0%
!!! TAAATTGCAAGTAATATTTT+TGG + chr6.3:42211626-42211645 MS.gene059414:intron 15.0%
!!! TTTTTACAATGAAAAAACTT+AGG + chr6.3:42211363-42211382 MS.gene059414:intron 15.0%
!! AACATATAAAAGAGGAAAAT+CGG - chr6.3:42212039-42212058 None:intergenic 20.0%
!! ACATAAACAAACATTTACAA+AGG - chr6.3:42212102-42212121 None:intergenic 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + chr6.3:42212112-42212131 MS.gene059414:intron 20.0%
!!! AATATTGTTTTTCTTCTTGT+AGG + chr6.3:42211762-42211781 MS.gene059414:intron 20.0%
!!! ATATTGTTTTTCTTCTTGTA+GGG + chr6.3:42211763-42211782 MS.gene059414:intron 20.0%
!!! TCTAAAGTATTCAACAATAA+GGG + chr6.3:42210153-42210172 MS.gene059414:CDS 20.0%
! AAAACTAAAGTCCATAATTC+AGG - chr6.3:42210207-42210226 None:intergenic 25.0%
! ACATATAAAAGAGGAAAATC+GGG - chr6.3:42212038-42212057 None:intergenic 25.0%
! AGTTGCATAACATATAAAAG+AGG - chr6.3:42212047-42212066 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + chr6.3:42210457-42210476 MS.gene059414:intron 25.0%
! ATTTGGCAATTGTTTATGAA+AGG + chr6.3:42210031-42210050 MS.gene059414:CDS 25.0%
! CTACCAAAAAACTAGAATAA+CGG - chr6.3:42210587-42210606 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+CGG + chr6.3:42211695-42211714 MS.gene059414:intron 25.0%
! TTTAAACATTCAGATTGGAT+AGG + chr6.3:42210117-42210136 MS.gene059414:CDS 25.0%
! TTTCTGTAATATGCTATTGA+TGG + chr6.3:42211562-42211581 MS.gene059414:intron 25.0%
!! GTCTAAAGTATTCAACAATA+AGG + chr6.3:42210152-42210171 MS.gene059414:CDS 25.0%
!!! GAGAATGATTTTTTCAATAG+CGG + chr6.3:42211144-42211163 MS.gene059414:intron 25.0%
!!! GTGTTTTTAAACATTCAGAT+TGG + chr6.3:42210112-42210131 MS.gene059414:CDS 25.0%
AAAATCACGATCAAACTGAT+GGG - chr6.3:42210900-42210919 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG + chr6.3:42210014-42210033 MS.gene059414:CDS 30.0%
ACTGTAATGGTGTGTTTATA+TGG - chr6.3:42210682-42210701 None:intergenic 30.0%
ATTGGATAGGAAAGTCATTT+GGG + chr6.3:42210130-42210149 MS.gene059414:CDS 30.0%
ATTGTAAAAATAAGCCACGT+TGG - chr6.3:42211353-42211372 None:intergenic 30.0%
CTATCTTCTATTTCACATAG+CGG + chr6.3:42211537-42211556 MS.gene059414:intron 30.0%
GACTTATTTCAACTGTTATG+CGG + chr6.3:42211493-42211512 MS.gene059414:intron 30.0%
GCTGATATACGAACTATATT+GGG + chr6.3:42211275-42211294 MS.gene059414:intron 30.0%
TATGTAATATTGCATCAGCT+AGG + chr6.3:42211063-42211082 MS.gene059414:intron 30.0%
TATTGTATTACGGTGAATAC+AGG - chr6.3:42211406-42211425 None:intergenic 30.0%
TGAAATAGAAGATAGTGTTG+CGG - chr6.3:42211532-42211551 None:intergenic 30.0%
TTGTAAAAATAAGCCACGTT+GGG - chr6.3:42211352-42211371 None:intergenic 30.0%
! AAAGTATTCAACAATAAGGG+CGG + chr6.3:42210156-42210175 MS.gene059414:CDS 30.0%
! AGACAAAAGAGAATTTTAGG+CGG + chr6.3:42210626-42210645 MS.gene059414:intron 30.0%
! AGCAGACAAAAGAGAATTTT+AGG + chr6.3:42210623-42210642 MS.gene059414:intron 30.0%
! ATACGATTTTCAGACTGTAA+TGG - chr6.3:42210695-42210714 None:intergenic 30.0%
! ATTTGATTTCCATGAGCATA+GGG + chr6.3:42211030-42211049 MS.gene059414:intron 30.0%
! CAGTCTGAAAATCGTATTTT+TGG + chr6.3:42210698-42210717 MS.gene059414:intron 30.0%
! GAAAGATATAAGGACTTTTG+AGG + chr6.3:42212122-42212141 MS.gene059414:intron 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - chr6.3:42210776-42210795 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG + chr6.3:42210263-42210282 MS.gene059414:CDS 30.0%
!!! AGTGTTGCAGTTTTTTTTAG+CGG - chr6.3:42210552-42210571 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + chr6.3:42210581-42210600 MS.gene059414:intron 30.0%
AAAGTCCATAATTCAGGAGT+AGG - chr6.3:42210201-42210220 None:intergenic 35.0%
AACGTATAAACATGTCCTGT+AGG - chr6.3:42210369-42210388 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG - chr6.3:42211104-42211123 None:intergenic 35.0%
ACCCTGCATGCAAATAAAAA+GGG - chr6.3:42211453-42211472 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTAA+AGG + chr6.3:42211724-42211743 MS.gene059414:intron 35.0%
AGCCATAACAGAAGAAGAAT+TGG - chr6.3:42212280-42212299 None:intergenic 35.0%
ATAAACATGCCCTTCACAAA+TGG - chr6.3:42211008-42211027 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG + chr6.3:42210942-42210961 MS.gene059414:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG + chr6.3:42210276-42210295 MS.gene059414:CDS 35.0%
CAAAATCACGATCAAACTGA+TGG - chr6.3:42210901-42210920 None:intergenic 35.0%
CGTTGACATTCTGAACAATA+GGG - chr6.3:42211662-42211681 None:intergenic 35.0%
CGTTGTACTTGTTCAATACA+CGG - chr6.3:42211977-42211996 None:intergenic 35.0%
GAAATAGAAGATAGTGTTGC+GGG - chr6.3:42211531-42211550 None:intergenic 35.0%
GATTGGATAGGAAAGTCATT+TGG + chr6.3:42210129-42210148 MS.gene059414:CDS 35.0%
GCAAAAATGTTACGACATGA+CGG + chr6.3:42211932-42211951 MS.gene059414:CDS 35.0%
GCATGCAAATAAAAAGGGAA+TGG - chr6.3:42211448-42211467 None:intergenic 35.0%
GCCATAACAGAAGAAGAATT+GGG - chr6.3:42212279-42212298 None:intergenic 35.0%
GGCTGATATACGAACTATAT+TGG + chr6.3:42211274-42211293 MS.gene059414:intron 35.0%
GGTTATCTTCCTTGAAATTC+CGG - chr6.3:42210755-42210774 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - chr6.3:42210754-42210773 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG - chr6.3:42211976-42211995 None:intergenic 35.0%
TATATAGAATCTGTGTCCTG+TGG - chr6.3:42210233-42210252 None:intergenic 35.0%
TCCCAATTCTTCTTCTGTTA+TGG + chr6.3:42212275-42212294 MS.gene059414:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG - chr6.3:42211975-42211994 None:intergenic 35.0%
TTTAACCACTTCACTTGCAA+GGG + chr6.3:42210335-42210354 MS.gene059414:CDS 35.0%
! CATTTGATTTCCATGAGCAT+AGG + chr6.3:42211029-42211048 MS.gene059414:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + chr6.3:42210390-42210409 MS.gene059414:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG + chr6.3:42210090-42210109 MS.gene059414:CDS 35.0%
! TTTCTAGTGTTGTCAGCTAT+TGG + chr6.3:42210498-42210517 MS.gene059414:intron 35.0%
!! TCCCTTTTTATTTGCATGCA+GGG + chr6.3:42211449-42211468 MS.gene059414:intron 35.0%
!! TTCCCTTTTTATTTGCATGC+AGG + chr6.3:42211448-42211467 MS.gene059414:intron 35.0%
!!! ATCGTATTTTTGGTGAAACG+TGG + chr6.3:42210708-42210727 MS.gene059414:intron 35.0%
AACAAAGATCTGGATCAGCT+AGG + chr6.3:42210400-42210419 MS.gene059414:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG + chr6.3:42212007-42212026 MS.gene059414:CDS 40.0%
ACCGCAGAAAATTGTAGGTA+CGG - chr6.3:42210803-42210822 None:intergenic 40.0%
ACTATAGTGCTATCACATGG+TGG + chr6.3:42211168-42211187 MS.gene059414:intron 40.0%
ATCAAACATCAGAGGAAGTC+AGG - chr6.3:42211096-42211115 None:intergenic 40.0%
CACCCTGCATGCAAATAAAA+AGG - chr6.3:42211454-42211473 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG + chr6.3:42210945-42210964 MS.gene059414:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG - chr6.3:42210947-42210966 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG + chr6.3:42210946-42210965 MS.gene059414:intron 40.0%
CTTTAACCACTTCACTTGCA+AGG + chr6.3:42210334-42210353 MS.gene059414:CDS 40.0%
GTATGGTCGGAATCAGAATT+TGG - chr6.3:42210843-42210862 None:intergenic 40.0%
TAGCTCCTACTCCTGAATTA+TGG + chr6.3:42210193-42210212 MS.gene059414:CDS 40.0%
TGACAACATGAAGCCAGTAA+CGG + chr6.3:42210056-42210075 MS.gene059414:CDS 40.0%
TGAGTCTACTATGAAAACCG+TGG + chr6.3:42211198-42211217 MS.gene059414:intron 40.0%
! ACCGTACCTACAATTTTCTG+CGG + chr6.3:42210799-42210818 MS.gene059414:intron 40.0%
! AGGGATGACTTTGAGAAGAT+TGG + chr6.3:42211782-42211801 MS.gene059414:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - chr6.3:42211926-42211945 None:intergenic 40.0%
! GCGTTGACATTCTGAACAAT+AGG - chr6.3:42211663-42211682 None:intergenic 40.0%
! GGCTGCTATTAAAGTTTTGC+TGG + chr6.3:42211253-42211272 MS.gene059414:intron 40.0%
! TCTGAGATTGACGGTAATGA+CGG + chr6.3:42212189-42212208 MS.gene059414:CDS 40.0%
! TGCTATTGATGGCACTTTCA+TGG + chr6.3:42211573-42211592 MS.gene059414:intron 40.0%
! TTTTGGATCTACCTCAACCT+TGG + chr6.3:42211891-42211910 MS.gene059414:CDS 40.0%
!! CGTATGATTTTGATACCCCT+TGG - chr6.3:42210875-42210894 None:intergenic 40.0%
!! CTATTGGCTCTATAGCATAG+CGG + chr6.3:42210514-42210533 MS.gene059414:intron 40.0%
!! GGACATACGAAGTTCTAGAA+GGG + chr6.3:42212154-42212173 MS.gene059414:CDS 40.0%
!! TTTTTGGTGAAACGTGGTCA+TGG + chr6.3:42210714-42210733 MS.gene059414:intron 40.0%
!!! ATTTTAGGCGGTGTTGTCAA+TGG + chr6.3:42210638-42210657 MS.gene059414:intron 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + chr6.3:42211874-42211893 MS.gene059414:CDS 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG + chr6.3:42210908-42210927 MS.gene059414:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + chr6.3:42210214-42210233 MS.gene059414:CDS 40.0%
AACATGCCCTTCACAAATGG+TGG - chr6.3:42211005-42211024 None:intergenic 45.0%
AAGCCAGTAACGGTATCTGA+GGG + chr6.3:42210066-42210085 MS.gene059414:CDS 45.0%
AGTAACGGTATCTGAGGGAT+CGG + chr6.3:42210071-42210090 MS.gene059414:CDS 45.0%
ATAAGCAGCTTAGTCACTGC+GGG + chr6.3:42211806-42211825 MS.gene059414:CDS 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + chr6.3:42212217-42212236 MS.gene059414:CDS 45.0%
CATAAGCAGCTTAGTCACTG+CGG + chr6.3:42211805-42211824 MS.gene059414:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG - chr6.3:42210948-42210967 None:intergenic 45.0%
CTAGAACTTCGTATGTCCGT+AGG - chr6.3:42212152-42212171 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+TGG - chr6.3:42210800-42210819 None:intergenic 45.0%
GGATCACCGCAGAAAATTGT+AGG - chr6.3:42210808-42210827 None:intergenic 45.0%
GGCACTATAGTGCTATCACA+TGG + chr6.3:42211165-42211184 MS.gene059414:intron 45.0%
GGGTTTCCTGAAGAATTTGC+TGG + chr6.3:42211851-42211870 MS.gene059414:CDS 45.0%
GGTCGGAATCAGAATTTGGT+GGG - chr6.3:42210839-42210858 None:intergenic 45.0%
TCCTTGAAATTCCGGGTTCA+CGG - chr6.3:42210747-42210766 None:intergenic 45.0%
TCTGTTATGGCTAGACCATG+CGG + chr6.3:42212288-42212307 MS.gene059414:CDS 45.0%
TGGTCGGAATCAGAATTTGG+TGG - chr6.3:42210840-42210859 None:intergenic 45.0%
TTTCGCTATCAGGACAGTAG+CGG + chr6.3:42211232-42211251 MS.gene059414:intron 45.0%
! ACAATTTTCTGCGGTGATCC+TGG + chr6.3:42210808-42210827 MS.gene059414:intron 45.0%
! ACTGTCCTGATAGCGAAACT+GGG - chr6.3:42211230-42211249 None:intergenic 45.0%
! AGAGGAAAATCGGGAAGTAC+CGG - chr6.3:42212029-42212048 None:intergenic 45.0%
! TACTGTCCTGATAGCGAAAC+TGG - chr6.3:42211231-42211250 None:intergenic 45.0%
! TTGAATCTGGCACTGGAAGT+GGG + chr6.3:42210310-42210329 MS.gene059414:CDS 45.0%
!! CGAAGTTCTAGAAGGGAAAG+TGG + chr6.3:42212161-42212180 MS.gene059414:CDS 45.0%
!! CGGACATACGAAGTTCTAGA+AGG + chr6.3:42212153-42212172 MS.gene059414:CDS 45.0%
!! GTGGAAAGCTCTGAGATTGA+CGG + chr6.3:42212180-42212199 MS.gene059414:CDS 45.0%
!! TCCTGAAGAATTTGCTGGCT+TGG + chr6.3:42211856-42211875 MS.gene059414:CDS 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG + chr6.3:42210914-42210933 MS.gene059414:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + chr6.3:42212133-42212152 MS.gene059414:CDS 45.0%
AAATTCCGGGTTCACGGGTT+GGG - chr6.3:42210741-42210760 None:intergenic 50.0%
AACTGCCCTTGCAAGTGAAG+TGG - chr6.3:42210343-42210362 None:intergenic 50.0%
AAGAGGAGGCAACGTTCAGA+TGG + chr6.3:42212234-42212253 MS.gene059414:CDS 50.0%
ACCATACTCCAGCATGCCAA+GGG + chr6.3:42210856-42210875 MS.gene059414:intron 50.0%
AGTGAGGGACATTCAAGGTG+AGG + chr6.3:42211829-42211848 MS.gene059414:CDS 50.0%
CCTTGAAATTCCGGGTTCAC+GGG - chr6.3:42210746-42210765 None:intergenic 50.0%
GAAGCCAGTAACGGTATCTG+AGG + chr6.3:42210065-42210084 MS.gene059414:CDS 50.0%
GATCCCTCAGATACCGTTAC+TGG - chr6.3:42210072-42210091 None:intergenic 50.0%
GATTCAAGAACGACGCAACC+AGG - chr6.3:42210297-42210316 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG + chr6.3:42211034-42211053 MS.gene059414:intron 50.0%
GCCAAGCCAGCAAATTCTTC+AGG - chr6.3:42211860-42211879 None:intergenic 50.0%
GCGGGCATCCCTTCATAAAT+TGG + chr6.3:42210936-42210955 MS.gene059414:intron 50.0%
GCTATCCCAGTTTCGCTATC+AGG + chr6.3:42211222-42211241 MS.gene059414:intron 50.0%
GGTTGCGTCGTTCTTGAATC+TGG + chr6.3:42210297-42210316 MS.gene059414:CDS 50.0%
GTCGTTCTTGAATCTGGCAC+TGG + chr6.3:42210303-42210322 MS.gene059414:CDS 50.0%
GTGAGGGACATTCAAGGTGA+GGG + chr6.3:42211830-42211849 MS.gene059414:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + chr6.3:42212220-42212239 MS.gene059414:CDS 50.0%
TGAGGGACATTCAAGGTGAG+GGG + chr6.3:42211831-42211850 MS.gene059414:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG - chr6.3:42210856-42210875 None:intergenic 50.0%
TTTGATACCCCTTGGCATGC+TGG - chr6.3:42210867-42210886 None:intergenic 50.0%
! CTTGAATCTGGCACTGGAAG+TGG + chr6.3:42210309-42210328 MS.gene059414:CDS 50.0%
! GACGGTAATGACGGCTCTAA+TGG + chr6.3:42212198-42212217 MS.gene059414:CDS 50.0%
! TTTGACTTCCTTGGCCATGG+CGG + chr6.3:42210917-42210936 MS.gene059414:intron 50.0%
!! TTAGGCGGTGTTGTCAATGG+TGG + chr6.3:42210641-42210660 MS.gene059414:intron 50.0%
ACCGTGGCCACCATTTGTGA+AGG + chr6.3:42210995-42211014 MS.gene059414:intron 55.0%
ACGTTCAGATGGAGCTAGCG+TGG + chr6.3:42212245-42212264 MS.gene059414:CDS 55.0%
AGACCATGCGGTGAAGCTAG+AGG + chr6.3:42212300-42212319 MS.gene059414:CDS 55.0%
ATCTGCATGTGTGCCCAACG+TGG + chr6.3:42211336-42211355 MS.gene059414:intron 55.0%
CCATACTCCAGCATGCCAAG+GGG + chr6.3:42210857-42210876 MS.gene059414:intron 55.0%
CCCGTGAACCCGGAATTTCA+AGG + chr6.3:42210743-42210762 MS.gene059414:intron 55.0%
CCCTTCACAAATGGTGGCCA+CGG - chr6.3:42210999-42211018 None:intergenic 55.0%
CCGTGGCCACCATTTGTGAA+GGG + chr6.3:42210996-42211015 MS.gene059414:intron 55.0%
CTGATCCAGCCCTATGCTCA+TGG - chr6.3:42211042-42211061 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG + chr6.3:42211814-42211833 MS.gene059414:CDS 55.0%
GAAATTCCGGGTTCACGGGT+TGG - chr6.3:42210742-42210761 None:intergenic 55.0%
GAACGTTGCCTCCTCTTGCA+AGG - chr6.3:42212231-42212250 None:intergenic 55.0%
GACCATACTCCAGCATGCCA+AGG + chr6.3:42210855-42210874 MS.gene059414:intron 55.0%
GCTGAAGGAATAGCGAGCCA+AGG - chr6.3:42211911-42211930 None:intergenic 55.0%
GGAATAGCGAGCCAAGGTTG+AGG - chr6.3:42211905-42211924 None:intergenic 55.0%
GGTGAAGCTAGAGGTCACAC+TGG + chr6.3:42212309-42212328 MS.gene059414:CDS 55.0%
TCTGCCTCACAATTCCACCG+TGG + chr6.3:42210979-42210998 MS.gene059414:intron 55.0%
TTATGAAGGGATGCCCGCCA+TGG - chr6.3:42210934-42210953 None:intergenic 55.0%
TTCACAAATGGTGGCCACGG+TGG - chr6.3:42210996-42211015 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+GGG + chr6.3:42210918-42210937 MS.gene059414:intron 55.0%
! AGAATTTGGTGGGTGCAGCC+AGG - chr6.3:42210829-42210848 None:intergenic 55.0%
! TGACCTCTAGCTTCACCGCA+TGG - chr6.3:42212306-42212325 None:intergenic 55.0%
!! GCGGTGTTGTCAATGGTGGA+AGG + chr6.3:42210645-42210664 MS.gene059414:intron 55.0%
!! TCGGGAAGTACCGGTGAAGT+TGG - chr6.3:42212020-42212039 None:intergenic 55.0%
ATGGACCCAACCCGTGAACC+CGG + chr6.3:42210733-42210752 MS.gene059414:intron 60.0%
CCCCTTGGCATGCTGGAGTA+TGG - chr6.3:42210860-42210879 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + chr6.3:42210351-42210370 MS.gene059414:CDS 60.0%
GCGAAACTGGGATAGCGCCA+CGG - chr6.3:42211218-42211237 None:intergenic 60.0%
GCGGGAGTGAGGGACATTCA+AGG + chr6.3:42211824-42211843 MS.gene059414:CDS 60.0%
GCTTAGTCACTGCGGGAGTG+AGG + chr6.3:42211813-42211832 MS.gene059414:CDS 60.0%
GTGGCCACGGTGGAATTGTG+AGG - chr6.3:42210986-42211005 None:intergenic 60.0%
AGGGATGCCCGCCATGGCCA+AGG - chr6.3:42210928-42210947 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 42209973 42212359 42209973 ID=MS.gene059414
chr6.3 mRNA 42209973 42212359 42209973 ID=MS.gene059414.t1;Parent=MS.gene059414
chr6.3 exon 42209973 42210421 42209973 ID=MS.gene059414.t1.exon1;Parent=MS.gene059414.t1
chr6.3 CDS 42209973 42210421 42209973 ID=cds.MS.gene059414.t1;Parent=MS.gene059414.t1
chr6.3 exon 42211784 42212028 42211784 ID=MS.gene059414.t1.exon2;Parent=MS.gene059414.t1
chr6.3 CDS 42211784 42212028 42211784 ID=cds.MS.gene059414.t1;Parent=MS.gene059414.t1
chr6.3 exon 42212127 42212359 42212127 ID=MS.gene059414.t1.exon3;Parent=MS.gene059414.t1
chr6.3 CDS 42212127 42212359 42212127 ID=cds.MS.gene059414.t1;Parent=MS.gene059414.t1
Gene Sequence

>MS.gene059414

ATGTTGGCTATCGATTCTACAAGAAAGATATCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGAAAGGCATGACAACATGAAGCCAGTAACGGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTTAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAACAATAAGGGCGGTTTTGTTTACTTATTAGCTCCTACTCCTGAATTATGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATATTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGCGTCGTTCTTGAATCTGGCACTGGAAGTGGGTCTTTAACCACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTGTCTTTCGCTGACGATATTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAGTGTTGTCAGCTATTGGCTCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGACAAAAGAGAATTTTAGGCGGTGTTGTCAATGGTGGAAGGCTAAAAATCTGCCATATAAACACACCATTACAGTCTGAAAATCGTATTTTTGGTGAAACGTGGTCATGGACCCAACCCGTGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCACCGTACCTACAATTTTCTGCGGTGATCCTGGCTGCACCCACCAAATTCTGATTCCGACCATACTCCAGCATGCCAAGGGGTATCAAAATCATACGATCCCATCAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCGGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCATTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATCAGCTATGTAATATTGCATCAGCTAGGTGCTGCACCTGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGAATGATTTTTTCAATAGCGGCACTATAGTGCTATCACATGGTGGATTTTGATGAGTCTACTATGAAAACCGTGGCGCTATCCCAGTTTCGCTATCAGGACAGTAGCGGCTGCTATTAAAGTTTTGCTGGCTGATATACGAACTATATTGGGAATTTTTTTCATTTTTTTTTAAAATAATAAAATTGAAGATCTGCATGTGTGCCCAACGTGGCTTATTTTTACAATGAAAAAACTTAGGAGTCAGTGAATATTACTCCTGTATTCACCGTAATACAATATATAAGTTACGTTCTGCTTCCATTCCCTTTTTATTTGCATGCAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTGCCCGCAACACTATCTTCTATTTCACATAGCGGATTTTCTGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTTTGTTCTCTTGTTGTATAAATTGCAAGTAATATTTTTGGTGATCTAATACCCTATTGTTCAGAATGTCAACGCTCAAGTTCAAATTTATTTAACTGTACTCAATGCGGTGATAAACTTGAGATGTTCAGTGTAAAGGTAAATTAAAGATATGAATATTGTTTTTCTTCTTGTAGGGATGACTTTGAGAAGATTGGCATAAGCAGCTTAGTCACTGCGGGAGTGAGGGACATTCAAGGTGAGGGGTTTCCTGAAGAATTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTCGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGGTACTTCCCGATTTTCCTCTTTTATATGTTATGCAACTAATGATAATTTTATTTACGCACTGACAAAAGTCCTTTGTAAATGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCTAGAAGGGAAAGTGGAAAGCTCTGAGATTGACGGTAATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAACGTTCAGATGGAGCTAGCGTGGTGAGCTATCCCAATTCTTCTTCTGTTATGGCTAGACCATGCGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MS.gene059414.t1

MLAIDSTRKISFNRTISNGDLAIVYERHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFNNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVLEGKVESSEIDGNDGSNGSLPCKRRQRSDGASVVSYPNSSSVMARPCGEARGHTGYLTFARLN