AlfalfaGEDB | MS.gene72748

M. sativa cultivar XinJiangDaYe / MS.gene72748

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72748.t1	XP_003612498.1	96.5	200	7	0	1	200	1	200	6.40E-88	333.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72748.t1	Q93Z27	40.1	197	103	6	14	200	13	204	5.1e-25	115.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72748.t1	G7K424	96.5	200	7	0	1	200	1	200	4.6e-88	333.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene72748	0.801882	7.30E-49	-1.69E-46
MS.gene04919	MS.gene72748	0.814993	1.16E-51	-1.69E-46
MS.gene04920	MS.gene72748	0.831224	1.88E-55	-1.69E-46
MS.gene049254	MS.gene72748	0.804987	1.66E-49	-1.69E-46
MS.gene049283	MS.gene72748	0.886707	2.71E-72	-1.69E-46
MS.gene049419	MS.gene72748	-0.813741	2.20E-51	-1.69E-46
MS.gene049499	MS.gene72748	0.836315	1.01E-56	-1.69E-46
MS.gene049861	MS.gene72748	0.80392	2.77E-49	-1.69E-46
MS.gene049983	MS.gene72748	0.859343	4.42E-63	-1.69E-46
MS.gene04998	MS.gene72748	0.853714	1.99E-61	-1.69E-46
MS.gene050271	MS.gene72748	0.827009	1.98E-54	-1.69E-46
MS.gene050414	MS.gene72748	0.815361	9.62E-52	-1.69E-46
MS.gene050571	MS.gene72748	0.803377	3.59E-49	-1.69E-46
MS.gene05059	MS.gene72748	0.803419	3.52E-49	-1.69E-46
MS.gene050654	MS.gene72748	0.838779	2.35E-57	-1.69E-46
MS.gene050941	MS.gene72748	0.801789	7.63E-49	-1.69E-46
MS.gene050945	MS.gene72748	0.80185	7.41E-49	-1.69E-46
MS.gene051122	MS.gene72748	0.820652	6.12E-53	-1.69E-46
MS.gene051184	MS.gene72748	0.831567	1.55E-55	-1.69E-46
MS.gene051275	MS.gene72748	0.815053	1.13E-51	-1.69E-46
MS.gene051343	MS.gene72748	0.803153	3.99E-49	-1.69E-46
MS.gene051541	MS.gene72748	0.813491	2.49E-51	-1.69E-46
MS.gene051597	MS.gene72748	0.838674	2.50E-57	-1.69E-46
MS.gene051676	MS.gene72748	0.823061	1.70E-53	-1.69E-46
MS.gene051699	MS.gene72748	0.81015	1.33E-50	-1.69E-46
MS.gene051831	MS.gene72748	0.801634	8.21E-49	-1.69E-46
MS.gene051902	MS.gene72748	0.805314	1.42E-49	-1.69E-46
MS.gene051975	MS.gene72748	0.81193	5.47E-51	-1.69E-46
MS.gene052296	MS.gene72748	0.800721	1.26E-48	-1.69E-46
MS.gene052342	MS.gene72748	0.802808	4.71E-49	-1.69E-46
MS.gene052533	MS.gene72748	0.80248	5.50E-49	-1.69E-46
MS.gene052586	MS.gene72748	0.805357	1.39E-49	-1.69E-46
MS.gene052685	MS.gene72748	0.815469	9.10E-52	-1.69E-46
MS.gene052954	MS.gene72748	0.806114	9.63E-50	-1.69E-46
MS.gene053006	MS.gene72748	0.810226	1.28E-50	-1.69E-46
MS.gene053045	MS.gene72748	0.80515	1.53E-49	-1.69E-46
MS.gene053058	MS.gene72748	0.808978	2.37E-50	-1.69E-46
MS.gene053094	MS.gene72748	0.806436	8.23E-50	-1.69E-46
MS.gene053295	MS.gene72748	0.831193	1.92E-55	-1.69E-46
MS.gene053298	MS.gene72748	0.879934	8.30E-70	-1.69E-46
MS.gene053340	MS.gene72748	0.82175	3.42E-53	-1.69E-46
MS.gene053456	MS.gene72748	0.820599	6.30E-53	-1.69E-46
MS.gene053564	MS.gene72748	0.816397	5.65E-52	-1.69E-46
MS.gene053575	MS.gene72748	0.802073	6.67E-49	-1.69E-46
MS.gene053726	MS.gene72748	0.833657	4.70E-56	-1.69E-46
MS.gene053786	MS.gene72748	0.80171	7.92E-49	-1.69E-46
MS.gene053879	MS.gene72748	0.835061	2.09E-56	-1.69E-46
MS.gene053931	MS.gene72748	0.806452	8.17E-50	-1.69E-46
MS.gene053963	MS.gene72748	0.88817	7.49E-73	-1.69E-46
MS.gene054222	MS.gene72748	0.843186	1.64E-58	-1.69E-46
MS.gene05439	MS.gene72748	0.832268	1.04E-55	-1.69E-46
MS.gene054479	MS.gene72748	0.820148	7.99E-53	-1.69E-46
MS.gene05469	MS.gene72748	0.812724	3.67E-51	-1.69E-46
MS.gene054892	MS.gene72748	0.875744	2.42E-68	-1.69E-46
MS.gene054893	MS.gene72748	0.882979	6.61E-71	-1.69E-46
MS.gene054957	MS.gene72748	0.839555	1.48E-57	-1.69E-46
MS.gene055147	MS.gene72748	0.803722	3.04E-49	-1.69E-46
MS.gene055150	MS.gene72748	0.821328	4.28E-53	-1.69E-46
MS.gene055222	MS.gene72748	0.844066	9.52E-59	-1.69E-46
MS.gene055226	MS.gene72748	0.832801	7.68E-56	-1.69E-46
MS.gene055513	MS.gene72748	0.800075	1.71E-48	-1.69E-46
MS.gene055841	MS.gene72748	0.817835	2.68E-52	-1.69E-46
MS.gene056045	MS.gene72748	0.801042	1.09E-48	-1.69E-46
MS.gene05624	MS.gene72748	0.812961	3.26E-51	-1.69E-46
MS.gene056699	MS.gene72748	0.806868	6.67E-50	-1.69E-46
MS.gene056776	MS.gene72748	0.822034	2.94E-53	-1.69E-46
MS.gene056779	MS.gene72748	0.80257	5.27E-49	-1.69E-46
MS.gene056849	MS.gene72748	0.812616	3.88E-51	-1.69E-46
MS.gene056986	MS.gene72748	0.828393	9.22E-55	-1.69E-46
MS.gene057102	MS.gene72748	0.825951	3.54E-54	-1.69E-46
MS.gene057155	MS.gene72748	0.855328	6.79E-62	-1.69E-46
MS.gene057208	MS.gene72748	0.835272	1.85E-56	-1.69E-46
MS.gene057622	MS.gene72748	0.867319	1.49E-65	-1.69E-46
MS.gene057890	MS.gene72748	-0.829201	5.88E-55	-1.69E-46
MS.gene057893	MS.gene72748	-0.828386	9.25E-55	-1.69E-46
MS.gene057999	MS.gene72748	0.853473	2.33E-61	-1.69E-46
MS.gene058008	MS.gene72748	0.803775	2.97E-49	-1.69E-46
MS.gene058159	MS.gene72748	0.837886	3.99E-57	-1.69E-46
MS.gene058223	MS.gene72748	-0.805272	1.45E-49	-1.69E-46
MS.gene058279	MS.gene72748	0.819955	8.85E-53	-1.69E-46
MS.gene058429	MS.gene72748	0.841791	3.83E-58	-1.69E-46
MS.gene058592	MS.gene72748	0.860748	1.66E-63	-1.69E-46
MS.gene058781	MS.gene72748	0.800386	1.48E-48	-1.69E-46
MS.gene05878	MS.gene72748	0.830953	2.20E-55	-1.69E-46
MS.gene059146	MS.gene72748	0.818475	1.92E-52	-1.69E-46
MS.gene059170	MS.gene72748	0.801746	7.79E-49	-1.69E-46
MS.gene05952	MS.gene72748	0.87909	1.65E-69	-1.69E-46
MS.gene05956	MS.gene72748	0.822951	1.80E-53	-1.69E-46
MS.gene05985	MS.gene72748	-0.841507	4.56E-58	-1.69E-46
MS.gene059872	MS.gene72748	-0.802796	4.73E-49	-1.69E-46
MS.gene059901	MS.gene72748	-0.804322	2.28E-49	-1.69E-46
MS.gene06011	MS.gene72748	0.814622	1.40E-51	-1.69E-46
MS.gene06018	MS.gene72748	0.810057	1.39E-50	-1.69E-46
MS.gene06023	MS.gene72748	0.812692	3.73E-51	-1.69E-46
MS.gene060259	MS.gene72748	0.872585	2.84E-67	-1.69E-46
MS.gene060412	MS.gene72748	0.854985	8.55E-62	-1.69E-46
MS.gene060413	MS.gene72748	0.812524	4.06E-51	-1.69E-46
MS.gene060426	MS.gene72748	0.805482	1.31E-49	-1.69E-46
MS.gene060520	MS.gene72748	0.811542	6.64E-51	-1.69E-46
MS.gene060840	MS.gene72748	0.824211	9.13E-54	-1.69E-46
MS.gene060850	MS.gene72748	0.811891	5.58E-51	-1.69E-46
MS.gene060851	MS.gene72748	0.829811	4.18E-55	-1.69E-46
MS.gene060852	MS.gene72748	0.829511	4.94E-55	-1.69E-46
MS.gene060932	MS.gene72748	0.830279	3.21E-55	-1.69E-46
MS.gene060933	MS.gene72748	0.803486	3.41E-49	-1.69E-46
MS.gene060935	MS.gene72748	0.81648	5.41E-52	-1.69E-46
MS.gene060937	MS.gene72748	0.835739	1.41E-56	-1.69E-46
MS.gene061047	MS.gene72748	0.801713	7.91E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72748.t1	MTR_5g025690	96.500	200	7	0	1	200	1	200	3.05e-137	382
MS.gene72748.t1	MTR_7g011010	79.126	206	33	2	1	200	1	202	1.15e-100	290
MS.gene72748.t1	MTR_4g127560	67.582	182	51	2	27	200	17	198	5.48e-75	224
MS.gene72748.t1	MTR_2g023310	82.353	51	9	0	83	133	47	97	1.40e-21	85.1
MS.gene72748.t1	MTR_4g082050	42.063	126	70	2	71	196	17	139	1.95e-21	86.3
MS.gene72748.t1	MTR_7g074240	55.385	65	29	0	131	195	106	170	2.68e-16	73.6
MS.gene72748.t1	MTR_2g098890	27.979	193	114	5	29	196	11	203	1.12e-13	67.4
MS.gene72748.t1	MTR_4g115170	44.595	74	41	0	124	197	145	218	3.22e-13	66.2
MS.gene72748.t1	MTR_4g067270	34.921	126	68	4	85	197	19	143	1.85e-12	62.8
MS.gene72748.t1	MTR_7g089760	47.541	61	32	0	136	196	82	142	2.50e-12	62.4
MS.gene72748.t1	MTR_8g107110	31.293	147	66	5	66	197	33	159	2.86e-12	62.4
MS.gene72748.t1	MTR_3g109320	36.667	120	67	2	84	195	60	178	5.57e-12	62.0
MS.gene72748.t1	MTR_3g067610	35.000	120	66	4	85	195	19	135	7.14e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72748.t1	AT5G39670	46.193	197	91	6	14	200	13	204	1.61e-44	147
MS.gene72748.t1	AT3G29000	60.550	109	41	1	89	197	85	191	6.00e-38	130
MS.gene72748.t1	AT4G20780	56.338	71	28	1	125	195	116	183	2.00e-16	74.3
MS.gene72748.t1	AT3G47480	56.061	66	28	1	131	196	115	179	3.27e-16	73.6
MS.gene72748.t1	AT5G44460	49.425	87	41	1	110	196	92	175	4.97e-16	72.8
MS.gene72748.t1	AT2G43290	41.573	89	51	1	108	196	120	207	9.83e-15	70.1
MS.gene72748.t1	AT3G59440	28.947	190	106	5	29	197	7	188	1.01e-14	69.7
MS.gene72748.t1	AT1G05990	47.692	65	34	0	133	197	80	144	6.29e-14	66.6
MS.gene72748.t1	AT3G07490	45.161	62	34	0	136	197	82	143	8.63e-13	63.5
MS.gene72748.t1	AT4G03290	46.875	64	34	0	133	196	82	145	1.45e-12	63.2
MS.gene72748.t1	AT4G12860	45.161	62	34	0	136	197	82	143	1.73e-11	60.1
MS.gene72748.t1	AT1G66410	49.254	67	32	1	129	195	46	110	8.01e-11	57.4
MS.gene72748.t1	AT1G66410	49.254	67	32	1	129	195	46	110	8.01e-11	57.4
MS.gene72748.t1	AT1G21550	34.234	111	66	2	85	191	41	148	9.25e-11	58.2

Find 23 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGAGTTATGTGTATTT+TGG	0.141163	5.3:+15293606	MS.gene72748:CDS
ACTTCATGTTCAACTTTCTT+TGG	0.236848	5.3:+15293217	MS.gene72748:CDS
GAGAAAGTGGAGAGATTAAG+AGG	0.257266	5.3:+15293392	MS.gene72748:CDS
TGAAATGGTGATGGCGAAAA+TGG	0.278918	5.3:+15293423	MS.gene72748:CDS
TGATGAGAATGAGCCAAGTT+TGG	0.401446	5.3:+15293516	MS.gene72748:CDS
ATCTTCACTTCCTCCAAACT+TGG	0.413419	5.3:-15293529	None:intergenic
CTTCTCTGCCAACTTCGTTC+CGG	0.421099	5.3:+15293313	MS.gene72748:CDS
TAAGAGGGATGAAGTTGAAA+TGG	0.443339	5.3:+15293408	MS.gene72748:CDS
AGAAGTAAAGGGAGAGAAGC+AGG	0.460203	5.3:+15293342	MS.gene72748:CDS
CTAAGTCGCCGGAACGAAGT+TGG	0.464546	5.3:-15293321	None:intergenic
TTCCGGCGACTTAGAAGTAA+AGG	0.467315	5.3:+15293330	MS.gene72748:CDS
TGAAATCAATTCTTCCATCA+TGG	0.475727	5.3:-15293690	None:intergenic
TCCCTTTACTTCTAAGTCGC+CGG	0.486137	5.3:-15293332	None:intergenic
TCAAATGTTAGCAGAGAAAG+TGG	0.493623	5.3:+15293379	MS.gene72748:CDS
AAGTTTGGAGGAAGTGAAGA+TGG	0.496634	5.3:+15293531	MS.gene72748:CDS
AGAAAGTGGAGAGATTAAGA+GGG	0.503717	5.3:+15293393	MS.gene72748:CDS
TGAGAATGAGCCAAGTTTGG+AGG	0.510803	5.3:+15293519	MS.gene72748:CDS
GAAATGGTGATGGCGAAAAT+GGG	0.513234	5.3:+15293424	MS.gene72748:CDS
CGGAACGAAGTTGGCAGAGA+AGG	0.517501	5.3:-15293312	None:intergenic
AGATGGATTTATTGATGCAA+AGG	0.523959	5.3:+15293579	MS.gene72748:CDS
ATTTGTTTGTCTTATTGAAG+AGG	0.533554	5.3:+15293162	MS.gene72748:CDS
TCCGGCGACTTAGAAGTAAA+GGG	0.551355	5.3:+15293331	MS.gene72748:CDS
GGATGAAGTTGAAATGGTGA+TGG	0.640448	5.3:+15293414	MS.gene72748:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACTGAATTTGTAAAAATTA+TGG	+	chr5.3:15293711-15293730	MS.gene72748:CDS	20.0%
!!!	AAAAGTTTTTCTCAACTTTT+TGG	+	chr5.3:15293284-15293303	MS.gene72748:CDS	20.0%
!	GATGAACTTGATGAAAAATA+TGG	+	chr5.3:15293469-15293488	MS.gene72748:CDS	25.0%
!!	ATTTGTTTGTCTTATTGAAG+AGG	+	chr5.3:15293162-15293181	MS.gene72748:CDS	25.0%
!!	CAAAGAGTTATGTGTATTTT+GGG	+	chr5.3:15293607-15293626	MS.gene72748:CDS	25.0%
!!!	AATTTTGATGAAAACCATGA+TGG	+	chr5.3:15293676-15293695	MS.gene72748:CDS	25.0%
	ACTTCATGTTCAACTTTCTT+TGG	+	chr5.3:15293217-15293236	MS.gene72748:CDS	30.0%
	TGAAATCAATTCTTCCATCA+TGG	-	chr5.3:15293693-15293712	None:intergenic	30.0%
!	AGATGGATTTATTGATGCAA+AGG	+	chr5.3:15293579-15293598	MS.gene72748:CDS	30.0%
!	GCAAAGAGTTATGTGTATTT+TGG	+	chr5.3:15293606-15293625	MS.gene72748:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	chr5.3:15293562-15293581	MS.gene72748:CDS	30.0%
!!!	TATGTGTATTTTGGGATTGA+AGG	+	chr5.3:15293615-15293634	MS.gene72748:CDS	30.0%
	AGAAAGTGGAGAGATTAAGA+GGG	+	chr5.3:15293393-15293412	MS.gene72748:CDS	35.0%
	TAAGAGGGATGAAGTTGAAA+TGG	+	chr5.3:15293408-15293427	MS.gene72748:CDS	35.0%
	TCAAATGTTAGCAGAGAAAG+TGG	+	chr5.3:15293379-15293398	MS.gene72748:CDS	35.0%
!!!	TGTATTTTGGGATTGAAGGA+AGG	+	chr5.3:15293619-15293638	MS.gene72748:CDS	35.0%
	AAGTTTGGAGGAAGTGAAGA+TGG	+	chr5.3:15293531-15293550	MS.gene72748:CDS	40.0%
	ATCTTCACTTCCTCCAAACT+TGG	-	chr5.3:15293532-15293551	None:intergenic	40.0%
	GAGAAAGTGGAGAGATTAAG+AGG	+	chr5.3:15293392-15293411	MS.gene72748:CDS	40.0%
	GGATGAAGTTGAAATGGTGA+TGG	+	chr5.3:15293414-15293433	MS.gene72748:CDS	40.0%
	TGATGAGAATGAGCCAAGTT+TGG	+	chr5.3:15293516-15293535	MS.gene72748:CDS	40.0%
!!	GAAATGGTGATGGCGAAAAT+GGG	+	chr5.3:15293424-15293443	MS.gene72748:CDS	40.0%
!!	TGAAATGGTGATGGCGAAAA+TGG	+	chr5.3:15293423-15293442	MS.gene72748:CDS	40.0%
	AGAAGTAAAGGGAGAGAAGC+AGG	+	chr5.3:15293342-15293361	MS.gene72748:CDS	45.0%
	TCCCTTTACTTCTAAGTCGC+CGG	-	chr5.3:15293335-15293354	None:intergenic	45.0%
	TCCGGCGACTTAGAAGTAAA+GGG	+	chr5.3:15293331-15293350	MS.gene72748:CDS	45.0%
	TGAGAATGAGCCAAGTTTGG+AGG	+	chr5.3:15293519-15293538	MS.gene72748:CDS	45.0%
	TTCCGGCGACTTAGAAGTAA+AGG	+	chr5.3:15293330-15293349	MS.gene72748:CDS	45.0%
	CTTCTCTGCCAACTTCGTTC+CGG	+	chr5.3:15293313-15293332	MS.gene72748:CDS	50.0%
	CGGAACGAAGTTGGCAGAGA+AGG	-	chr5.3:15293315-15293334	None:intergenic	55.0%
	CTAAGTCGCCGGAACGAAGT+TGG	-	chr5.3:15293324-15293343	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	15293148	15293750	15293148	ID=MS.gene72748
chr5.3	mRNA	15293148	15293750	15293148	ID=MS.gene72748.t1;Parent=MS.gene72748
chr5.3	exon	15293148	15293750	15293148	ID=MS.gene72748.t1.exon1;Parent=MS.gene72748.t1
chr5.3	CDS	15293148	15293750	15293148	ID=cds.MS.gene72748.t1;Parent=MS.gene72748.t1

Gene Sequence

>MS.gene72748

ATGGTTTTTAGTCAATTTGTTTGTCTTATTGAAGAGGAGAAGACATCACAGAATGATGATAGCAACTCAACTTCATGTTCAACTTTCTTTGGTTTGATAGATTTTTTCATATATTCAACAGTATTCATCAGAATACAAAAGTTTTTCTCAACTTTTTGGTTTTTCCTTCTCTGCCAACTTCGTTCCGGCGACTTAGAAGTAAAGGGAGAGAAGCAGGTATCAGAAACTGAATCAAATGTTAGCAGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAATGGTGATGGCGAAAATGGGAATTTTTTGCAGCTCAGAAAGTGATGAACTTGATGAAAAATATGGTTCAAATGAACTCTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTTGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAAAGGAATTGCAAAGAGTTATGTGTATTTTGGGATTGAAGGAAGGTTTAGAAGTTAAGAATTGTCAGAAGATGATCAAGAATTTTGATGAAAACCATGATGGAAGAATTGATTTCACTGAATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>MS.gene72748.t1

MVFSQFVCLIEEEKTSQNDDSNSTSCSTFFGLIDFFIYSTVFIRIQKFFSTFWFFLLCQLRSGDLEVKGEKQVSETESNVSRESGEIKRDEVEMVMAKMGIFCSSESDELDEKYGSNELSELFDENEPSLEEVKMAFDVFDENKDGFIDAKELQRVMCILGLKEGLEVKNCQKMIKNFDENHDGRIDFTEFVKIMENRLC