Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060427.t1 | XP_004508161.1 | 98.4 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 3.40E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060427.t1 | Q9M7X7 | 83.6 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 8.8e-21 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060427.t1 | I3SZB6 | 98.4 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 2.5e-24 | 120.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene043593 | MS.gene060427 | PPI |
| MS.gene060427 | MS.gene043595 | PPI |
| MS.gene064353 | MS.gene060427 | PPI |
| MS.gene060427 | MS.gene73093 | PPI |
| MS.gene035826 | MS.gene060427 | PPI |
| MS.gene060427 | MS.gene035825 | PPI |
| MS.gene064351 | MS.gene060427 | PPI |
| MS.gene99872 | MS.gene060427 | PPI |
| MS.gene043643 | MS.gene060427 | PPI |
| MS.gene035828 | MS.gene060427 | PPI |
| MS.gene043645 | MS.gene060427 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060427.t1 | MTR_4g121877 | 98.361 | 61 | 1 | 0 | 1 | 61 | 1 | 61 | 7.37e-39 | 122 |
| MS.gene060427.t1 | MTR_4g121870 | 93.443 | 61 | 4 | 0 | 1 | 61 | 137 | 197 | 1.26e-36 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060427.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene060427.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene060427.t1 | AT3G06700 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 2.28e-31 | 103 |
| MS.gene060427.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 23 | 83 | 2.54e-31 | 104 |
| MS.gene060427.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 4.88e-31 | 102 |
| MS.gene060427.t1 | AT3G06680 | 83.607 | 61 | 10 | 0 | 1 | 61 | 1 | 61 | 4.88e-31 | 102 |
Find 14 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGGCGATGTCTCTT+TGG | 0.360027 | 7.1:-66469405 | None:intergenic |
| CTGATAAATACAAACTCCTT+TGG | 0.392523 | 7.1:-66469430 | None:intergenic |
| CTCTGGTTCCTCAAGAACTT+TGG | 0.404366 | 7.1:-66469536 | None:intergenic |
| TCTTGTGAGCCTTGTAAGAC+TGG | 0.428046 | 7.1:-66469368 | None:intergenic |
| TGTGCTTCCTTGCATACCTC+TGG | 0.438138 | 7.1:-66469553 | None:intergenic |
| CGCTCACAACCAGTCTTACA+AGG | 0.489194 | 7.1:+66469359 | MS.gene060427:CDS |
| ATAAATACAAACTCCTTTGG+TGG | 0.508253 | 7.1:-66469427 | None:intergenic |
| TCTTACAAGGCTCACAAGAA+TGG | 0.521704 | 7.1:+66469372 | MS.gene060427:CDS |
| AACAATTTACTCTTCTTCAG+TGG | 0.567212 | 7.1:-66469597 | None:intergenic |
| AGATGGATCCAAAGTTCTTG+AGG | 0.579402 | 7.1:+66469528 | MS.gene060427:intron |
| CATCGCCACACTTCCACCAA+AGG | 0.596196 | 7.1:+66469414 | MS.gene060427:CDS |
| AAACTCCTTTGGTGGAAGTG+TGG | 0.614881 | 7.1:-66469419 | None:intergenic |
| TGAGGAACCAGAGGTATGCA+AGG | 0.624057 | 7.1:+66469546 | MS.gene060427:CDS |
| CAAAGTTCTTGAGGAACCAG+AGG | 0.651910 | 7.1:+66469537 | MS.gene060427:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTTTATAATTGTAGA+TGG | + | chr7.1:66469511-66469530 | MS.gene060427:intron | 10.0% |
| ATAAATACAAACTCCTTTGG+TGG | - | chr7.1:66469430-66469449 | None:intergenic | 30.0% | |
| CTGATAAATACAAACTCCTT+TGG | - | chr7.1:66469433-66469452 | None:intergenic | 30.0% | |
| ! | AGGAAGCACAACAAGAAAAA+CGG | + | chr7.1:66469566-66469585 | MS.gene060427:CDS | 35.0% |
| AGATGGATCCAAAGTTCTTG+AGG | + | chr7.1:66469528-66469547 | MS.gene060427:intron | 40.0% | |
| TCTTACAAGGCTCACAAGAA+TGG | + | chr7.1:66469372-66469391 | MS.gene060427:CDS | 40.0% | |
| AAACTCCTTTGGTGGAAGTG+TGG | - | chr7.1:66469422-66469441 | None:intergenic | 45.0% | |
| CAAAGTTCTTGAGGAACCAG+AGG | + | chr7.1:66469537-66469556 | MS.gene060427:CDS | 45.0% | |
| ! | CTCTGGTTCCTCAAGAACTT+TGG | - | chr7.1:66469539-66469558 | None:intergenic | 45.0% |
| ! | TCTTGTGAGCCTTGTAAGAC+TGG | - | chr7.1:66469371-66469390 | None:intergenic | 45.0% |
| CGCTCACAACCAGTCTTACA+AGG | + | chr7.1:66469359-66469378 | MS.gene060427:CDS | 50.0% | |
| GAAGTGTGGCGATGTCTCTT+TGG | - | chr7.1:66469408-66469427 | None:intergenic | 50.0% | |
| TGTGCTTCCTTGCATACCTC+TGG | - | chr7.1:66469556-66469575 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | + | chr7.1:66469546-66469565 | MS.gene060427:CDS | 50.0% |
| CATCGCCACACTTCCACCAA+AGG | + | chr7.1:66469414-66469433 | MS.gene060427:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 66469336 | 66469613 | 66469336 | ID=MS.gene060427 |
| chr7.1 | mRNA | 66469336 | 66469613 | 66469336 | ID=MS.gene060427.t1;Parent=MS.gene060427 |
| chr7.1 | exon | 66469336 | 66469437 | 66469336 | ID=MS.gene060427.t1.exon1;Parent=MS.gene060427.t1 |
| chr7.1 | CDS | 66469336 | 66469437 | 66469336 | ID=cds.MS.gene060427.t1;Parent=MS.gene060427.t1 |
| chr7.1 | exon | 66469530 | 66469613 | 66469530 | ID=MS.gene060427.t1.exon2;Parent=MS.gene060427.t1 |
| chr7.1 | CDS | 66469530 | 66469613 | 66469530 | ID=cds.MS.gene060427.t1;Parent=MS.gene060427.t1 |
| Gene Sequence |
| Protein sequence |
