AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93654


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93654.t1 MTR_7g066870 58.628 452 97 10 1 374 421 860 1.08e-140 421
MS.gene93654.t1 MTR_7g066870 59.600 250 58 3 1 249 378 585 2.83e-68 225
MS.gene93654.t1 MTR_7g066870 83.582 67 11 0 41 107 520 586 3.61e-19 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93654.t1 AT2G38040 42.297 357 178 8 1 342 383 726 9.43e-70 233
MS.gene93654.t1 AT2G38040 42.297 357 178 8 1 342 383 726 9.43e-70 233

Find 64 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCTTTGATTCCGAATT+TGG 0.097093 7.1:-34794013 None:intergenic
ACTAAACAAGGATGTGAAAA+AGG 0.281837 7.1:+34793904 MS.gene93654:CDS
CGCATCCTCAGCAAGCGATT+TGG 0.311917 7.1:+34793736 MS.gene93654:CDS
CCCTTCAGCAATGGATCAAT+AGG 0.337322 7.1:-34793470 None:intergenic
CCCCAGAGGTTTCAGACATC+AGG 0.360996 7.1:-34794117 None:intergenic
AAAGAAACTGGAAGAGAAAC+TGG 0.369228 7.1:+34793340 MS.gene93654:CDS
TTCTTCAGCTTGTTAGCATT+AGG 0.371644 7.1:-34793542 None:intergenic
ATTTGGTGTCCCTCCAGCTT+TGG 0.385342 7.1:-34793996 None:intergenic
GAAAATCAATGAGTTCAATA+AGG 0.405814 7.1:+34793775 MS.gene93654:CDS
AGATGAGGCGATAAAGAAAC+TGG 0.418414 7.1:+34793328 MS.gene93654:CDS
AGCTGGAGGGACACCAAATT+CGG 0.428121 7.1:+34794000 MS.gene93654:CDS
GTCAGTGGCCGCTAAAGCCT+TGG 0.432082 7.1:+34793385 MS.gene93654:CDS
CCTCCTTGTTTCTGTCTGAC+AGG 0.436937 7.1:-34793607 None:intergenic
AAAGTGGAGGTCGCCAAAGC+TGG 0.442807 7.1:+34793983 MS.gene93654:CDS
GCCTCATCTAAATCGAGTTT+CGG 0.448664 7.1:-34793314 None:intergenic
TCAGTGGCCGCTAAAGCCTT+GGG 0.450053 7.1:+34793386 MS.gene93654:CDS
ACCTGATGTCTGAAACCTCT+GGG 0.457541 7.1:+34794116 MS.gene93654:CDS
AACTCGTTGCCATCTCGTCC+TGG 0.464998 7.1:-34793860 None:intergenic
GTTGAACTCACATTAAGAAA+AGG 0.472332 7.1:-34794209 None:intergenic
AACCTGATGTCTGAAACCTC+TGG 0.484029 7.1:+34794115 MS.gene93654:CDS
AATGATGGAGCAATTGAAAG+TGG 0.495125 7.1:+34793967 MS.gene93654:CDS
GGAAGTTTGAAAGTAGGAAA+TGG 0.497527 7.1:+34794142 MS.gene93654:CDS
CAGAGCCTTGCAGAGGCCGT+AGG 0.500417 7.1:+34794064 MS.gene93654:CDS
GCTAATGTTGTGAAGTCGGT+CGG 0.507927 7.1:+34793815 MS.gene93654:CDS
TTGTTTAGTTCTTCTATCTG+AGG 0.510370 7.1:-34793890 None:intergenic
TGTTAAGCCCAAGGCTTTAG+CGG 0.511334 7.1:-34793393 None:intergenic
ACCTATTGATCCATTGCTGA+AGG 0.534858 7.1:+34793469 MS.gene93654:CDS
AACAGATATTAAGAGAATGA+TGG 0.535873 7.1:+34793952 MS.gene93654:CDS
TCTATCTTACCCTTCAGCAA+TGG 0.536921 7.1:-34793479 None:intergenic
CTAATGTTGTGAAGTCGGTC+GGG 0.541128 7.1:+34793816 MS.gene93654:CDS
CAAACTTTCTGTTAAGCCCA+AGG 0.551454 7.1:-34793402 None:intergenic
ATGTCTGAAACCTCTGGGGA+TGG 0.551656 7.1:+34794121 MS.gene93654:CDS
ACTGGAAGAGAAACTGGAAA+AGG 0.562500 7.1:+34793346 MS.gene93654:CDS
GGGGATGGAAGTTTGAAAGT+AGG 0.564045 7.1:+34794136 MS.gene93654:CDS
TTTCTCTCTCAACTCGTCAT+CGG 0.564143 7.1:-34794170 None:intergenic
CTGAGGATGCGCTAACACGC+TGG 0.568228 7.1:-34793724 None:intergenic
CAGTTGCTGCGTAAGTGTTG+TGG 0.571821 7.1:-34793630 None:intergenic
GTGGAGGTCGCCAAAGCTGG+AGG 0.581724 7.1:+34793986 MS.gene93654:CDS
TTAGTAAAACCAGGACGAGA+TGG 0.587670 7.1:+34793851 MS.gene93654:CDS
TCAGCAATGGATCAATAGGT+TGG 0.591042 7.1:-34793466 None:intergenic
GACGAGTTGAGAGAGAAAGT+TGG 0.597575 7.1:+34794175 MS.gene93654:CDS
TTTCAAACTAGAAGAGCCTA+CGG 0.606963 7.1:-34794080 None:intergenic
AAAATCGGTGGGTTCAAGGA+AGG 0.607904 7.1:+34793158 MS.gene93654:CDS
ATCATCCAAATCGCTTGCTG+AGG 0.612600 7.1:-34793741 None:intergenic
CCTATTGATCCATTGCTGAA+GGG 0.615117 7.1:+34793470 MS.gene93654:CDS
AACAAGCTGAAGAAGAAGCA+TGG 0.618458 7.1:+34793551 MS.gene93654:CDS
TGGAGGTCGCCAAAGCTGGA+GGG 0.627204 7.1:+34793987 MS.gene93654:CDS
GATGGCTAATGTTGTGAAGT+CGG 0.628322 7.1:+34793811 MS.gene93654:CDS
GATGTTCAGTTAGTAAAACC+AGG 0.630796 7.1:+34793842 MS.gene93654:CDS
AATCAATGAGTTCAATAAGG+AGG 0.631088 7.1:+34793778 MS.gene93654:CDS
AAGAGCCTACGGCCTCTGCA+AGG 0.633658 7.1:-34794069 None:intergenic
GGAAGAGAAACTGGAAAAGG+AGG 0.638755 7.1:+34793349 MS.gene93654:CDS
TCTCCTGTCAGACAGAAACA+AGG 0.645421 7.1:+34793604 MS.gene93654:CDS
TCATCTAAATCGAGTTTCGG+AGG 0.646059 7.1:-34793311 None:intergenic
TCCGAAACTCGATTTAGATG+AGG 0.652577 7.1:+34793313 MS.gene93654:CDS
GAGTTTCGGAGGCTCAGAAG+AGG 0.652851 7.1:-34793300 None:intergenic
TAAGGAGGTAGACTTGCAGA+TGG 0.656254 7.1:+34793793 MS.gene93654:CDS
AATAAAGCAGAGCCTTGCAG+AGG 0.662404 7.1:+34794057 MS.gene93654:CDS
TCAGATAGAAGAACTAAACA+AGG 0.670049 7.1:+34793892 MS.gene93654:CDS
TTTCAAACTTCCATCCCCAG+AGG 0.689826 7.1:-34794131 None:intergenic
GGTTGATCAAGAATTGTCAG+TGG 0.698128 7.1:+34793370 MS.gene93654:CDS
GATGGAGCAATTGAAAGTGG+AGG 0.703918 7.1:+34793970 MS.gene93654:CDS
CCTGATGTCTGAAACCTCTG+GGG 0.731070 7.1:+34794117 MS.gene93654:CDS
CCTGTCAGACAGAAACAAGG+AGG 0.745350 7.1:+34793607 MS.gene93654:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAATTTATATCAAACTT+TGG + chr7.1:34793062-34793081 MS.gene93654:intron 15.0%
!! TAGAATAAAGAAAAACTATG+AGG + chr7.1:34793685-34793704 MS.gene93654:CDS 20.0%
!!! TCATAGTTTTTCTTTATTCT+AGG - chr7.1:34793686-34793705 None:intergenic 20.0%
!!! TTCATGTTTCTTTTTTTCTT+GGG - chr7.1:34793197-34793216 None:intergenic 20.0%
! AAAAAAGAAACATGAAGAAG+AGG + chr7.1:34793201-34793220 MS.gene93654:CDS 25.0%
! AAAAAGAAACATGAAGAAGA+GGG + chr7.1:34793202-34793221 MS.gene93654:CDS 25.0%
! AACAGATATTAAGAGAATGA+TGG + chr7.1:34793952-34793971 MS.gene93654:CDS 25.0%
! GAAAATCAATGAGTTCAATA+AGG + chr7.1:34793775-34793794 MS.gene93654:CDS 25.0%
!! GAAAAAGGAAATTGAAGTTT+TGG + chr7.1:34793919-34793938 MS.gene93654:CDS 25.0%
!!! CTTCATGTTTCTTTTTTTCT+TGG - chr7.1:34793198-34793217 None:intergenic 25.0%
AATCAATGAGTTCAATAAGG+AGG + chr7.1:34793778-34793797 MS.gene93654:CDS 30.0%
ACTAAACAAGGATGTGAAAA+AGG + chr7.1:34793904-34793923 MS.gene93654:CDS 30.0%
ATGCATAAGTTCCGAAAAAT+CGG + chr7.1:34793143-34793162 MS.gene93654:CDS 30.0%
CTATTCTTTGATTCCGAATT+TGG - chr7.1:34794016-34794035 None:intergenic 30.0%
GGGTAAGATAGAAAAACTAA+AGG + chr7.1:34793490-34793509 MS.gene93654:CDS 30.0%
TCAGATAGAAGAACTAAACA+AGG + chr7.1:34793892-34793911 MS.gene93654:CDS 30.0%
! TCAGAATCAGAAATTTTCTC+AGG - chr7.1:34793236-34793255 None:intergenic 30.0%
!! TAAAGGCAGATTTTAATCGA+AGG + chr7.1:34793507-34793526 MS.gene93654:CDS 30.0%
!! TTGTTTAGTTCTTCTATCTG+AGG - chr7.1:34793893-34793912 None:intergenic 30.0%
AAAGAAACTGGAAGAGAAAC+TGG + chr7.1:34793340-34793359 MS.gene93654:CDS 35.0%
ATAAGTTCCGAAAAATCGGT+GGG + chr7.1:34793147-34793166 MS.gene93654:CDS 35.0%
GATGTTCAGTTAGTAAAACC+AGG + chr7.1:34793842-34793861 MS.gene93654:CDS 35.0%
GGAAGTTTGAAAGTAGGAAA+TGG + chr7.1:34794142-34794161 MS.gene93654:CDS 35.0%
TTTCAAACTAGAAGAGCCTA+CGG - chr7.1:34794083-34794102 None:intergenic 35.0%
! AATGATGGAGCAATTGAAAG+TGG + chr7.1:34793967-34793986 MS.gene93654:CDS 35.0%
! AGAAAAACTATGAGGCGTTA+CGG + chr7.1:34793693-34793712 MS.gene93654:CDS 35.0%
! GAATCAGAAATTTTCTCAGG+AGG - chr7.1:34793233-34793252 None:intergenic 35.0%
!! TTCTTCAGCTTGTTAGCATT+AGG - chr7.1:34793545-34793564 None:intergenic 35.0%
!!! CTTTTTTTCTTGGGATCTAC+AGG - chr7.1:34793188-34793207 None:intergenic 35.0%
!!! TATGCATGCGATGTTTTAAC+AGG - chr7.1:34793130-34793149 None:intergenic 35.0%
AACAAGCTGAAGAAGAAGCA+TGG + chr7.1:34793551-34793570 MS.gene93654:CDS 40.0%
ACCTATTGATCCATTGCTGA+AGG + chr7.1:34793469-34793488 MS.gene93654:CDS 40.0%
ACTGGAAGAGAAACTGGAAA+AGG + chr7.1:34793346-34793365 MS.gene93654:CDS 40.0%
AGATGAGGCGATAAAGAAAC+TGG + chr7.1:34793328-34793347 MS.gene93654:CDS 40.0%
CAAACTTTCTGTTAAGCCCA+AGG - chr7.1:34793405-34793424 None:intergenic 40.0%
CATAAGTTCCGAAAAATCGG+TGG + chr7.1:34793146-34793165 MS.gene93654:CDS 40.0%
CCTATTGATCCATTGCTGAA+GGG + chr7.1:34793470-34793489 MS.gene93654:CDS 40.0%
GATGGCTAATGTTGTGAAGT+CGG + chr7.1:34793811-34793830 MS.gene93654:CDS 40.0%
GCCTCATCTAAATCGAGTTT+CGG - chr7.1:34793317-34793336 None:intergenic 40.0%
GGTTGATCAAGAATTGTCAG+TGG + chr7.1:34793370-34793389 MS.gene93654:CDS 40.0%
TCAGCAATGGATCAATAGGT+TGG - chr7.1:34793469-34793488 None:intergenic 40.0%
TCATCTAAATCGAGTTTCGG+AGG - chr7.1:34793314-34793333 None:intergenic 40.0%
TCCGAAACTCGATTTAGATG+AGG + chr7.1:34793313-34793332 MS.gene93654:CDS 40.0%
TCTATCTTACCCTTCAGCAA+TGG - chr7.1:34793482-34793501 None:intergenic 40.0%
TGAGTTCCTACACAATGAAC+AGG - chr7.1:34793088-34793107 None:intergenic 40.0%
TTAGTAAAACCAGGACGAGA+TGG + chr7.1:34793851-34793870 MS.gene93654:CDS 40.0%
TTTCTCTCTCAACTCGTCAT+CGG - chr7.1:34794173-34794192 None:intergenic 40.0%
AATAAAGCAGAGCCTTGCAG+AGG + chr7.1:34794057-34794076 MS.gene93654:CDS 45.0%
CCCTTCAGCAATGGATCAAT+AGG - chr7.1:34793473-34793492 None:intergenic 45.0%
CTAATGTTGTGAAGTCGGTC+GGG + chr7.1:34793816-34793835 MS.gene93654:CDS 45.0%
CTTTGGCCTGTTCATTGTGT+AGG + chr7.1:34793079-34793098 MS.gene93654:intron 45.0%
GATGGAGCAATTGAAAGTGG+AGG + chr7.1:34793970-34793989 MS.gene93654:CDS 45.0%
GCTAATGTTGTGAAGTCGGT+CGG + chr7.1:34793815-34793834 MS.gene93654:CDS 45.0%
GGAAGAGAAACTGGAAAAGG+AGG + chr7.1:34793349-34793368 MS.gene93654:CDS 45.0%
GGGGATGGAAGTTTGAAAGT+AGG + chr7.1:34794136-34794155 MS.gene93654:CDS 45.0%
TAAGGAGGTAGACTTGCAGA+TGG + chr7.1:34793793-34793812 MS.gene93654:CDS 45.0%
TCTCCTGTCAGACAGAAACA+AGG + chr7.1:34793604-34793623 MS.gene93654:CDS 45.0%
TTTCAAACTTCCATCCCCAG+AGG - chr7.1:34794134-34794153 None:intergenic 45.0%
! AAAATCGGTGGGTTCAAGGA+AGG + chr7.1:34793158-34793177 MS.gene93654:CDS 45.0%
! AACCTGATGTCTGAAACCTC+TGG + chr7.1:34794115-34794134 MS.gene93654:CDS 45.0%
! ACCTGATGTCTGAAACCTCT+GGG + chr7.1:34794116-34794135 MS.gene93654:CDS 45.0%
! ACGAGATGGCAACGAGTTTT+TGG + chr7.1:34793865-34793884 MS.gene93654:CDS 45.0%
! ATCATCCAAATCGCTTGCTG+AGG - chr7.1:34793744-34793763 None:intergenic 45.0%
! GACGAGTTGAGAGAGAAAGT+TGG + chr7.1:34794175-34794194 MS.gene93654:CDS 45.0%
! TGTTAAGCCCAAGGCTTTAG+CGG - chr7.1:34793396-34793415 None:intergenic 45.0%
!!! CCTTGAACCCACCGATTTTT+CGG - chr7.1:34793157-34793176 None:intergenic 45.0%
AGCTGGAGGGACACCAAATT+CGG + chr7.1:34794000-34794019 MS.gene93654:CDS 50.0%
CCTGTCAGACAGAAACAAGG+AGG + chr7.1:34793607-34793626 MS.gene93654:CDS 50.0%
! ATGTCTGAAACCTCTGGGGA+TGG + chr7.1:34794121-34794140 MS.gene93654:CDS 50.0%
! CAGTTGCTGCGTAAGTGTTG+TGG - chr7.1:34793633-34793652 None:intergenic 50.0%
! CCGAAAAATCGGTGGGTTCA+AGG + chr7.1:34793154-34793173 MS.gene93654:CDS 50.0%
! CCTGATGTCTGAAACCTCTG+GGG + chr7.1:34794117-34794136 MS.gene93654:CDS 50.0%
!! ATTTGGTGTCCCTCCAGCTT+TGG - chr7.1:34793999-34794018 None:intergenic 50.0%
!! CCTCCTTGTTTCTGTCTGAC+AGG - chr7.1:34793610-34793629 None:intergenic 50.0%
AAAGTGGAGGTCGCCAAAGC+TGG + chr7.1:34793983-34794002 MS.gene93654:CDS 55.0%
AACTCGTTGCCATCTCGTCC+TGG - chr7.1:34793863-34793882 None:intergenic 55.0%
CCCCAGAGGTTTCAGACATC+AGG - chr7.1:34794120-34794139 None:intergenic 55.0%
CGCATCCTCAGCAAGCGATT+TGG + chr7.1:34793736-34793755 MS.gene93654:CDS 55.0%
GAGTTTCGGAGGCTCAGAAG+AGG - chr7.1:34793303-34793322 None:intergenic 55.0%
TCAGTGGCCGCTAAAGCCTT+GGG + chr7.1:34793386-34793405 MS.gene93654:CDS 55.0%
AAGAGCCTACGGCCTCTGCA+AGG - chr7.1:34794072-34794091 None:intergenic 60.0%
CTGAGGATGCGCTAACACGC+TGG - chr7.1:34793727-34793746 None:intergenic 60.0%
GTCAGTGGCCGCTAAAGCCT+TGG + chr7.1:34793385-34793404 MS.gene93654:CDS 60.0%
TGGAGGTCGCCAAAGCTGGA+GGG + chr7.1:34793987-34794006 MS.gene93654:CDS 60.0%
CAGAGCCTTGCAGAGGCCGT+AGG + chr7.1:34794064-34794083 MS.gene93654:CDS 65.0%
GTGGAGGTCGCCAAAGCTGG+AGG + chr7.1:34793986-34794005 MS.gene93654:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 34793000 34794219 34793000 ID=MS.gene93654
chr7.1 mRNA 34793000 34794219 34793000 ID=MS.gene93654.t1;Parent=MS.gene93654
chr7.1 exon 34793000 34793005 34793000 ID=MS.gene93654.t1.exon1;Parent=MS.gene93654.t1
chr7.1 CDS 34793000 34793005 34793000 ID=cds.MS.gene93654.t1;Parent=MS.gene93654.t1
chr7.1 exon 34793101 34794219 34793101 ID=MS.gene93654.t1.exon2;Parent=MS.gene93654.t1
chr7.1 CDS 34793101 34794219 34793101 ID=cds.MS.gene93654.t1;Parent=MS.gene93654.t1
Gene Sequence

>MS.gene93654

ATGGATGTAAGTTTTACTGGCATGATGTGTTTTACAGAAATCAAATATGTTGAAGCATATGCTGATAATTTATATCAAACTTTGGCCTGTTCATTGTGTAGGAACTCACTAAGATGAACACAGAAGACCTGTTAAAACATCGCATGCATAAGTTCCGAAAAATCGGTGGGTTCAAGGAAGGAGTTCCTGTAGATCCCAAGAAAAAAAGAAACATGAAGAAGAGGGATATACCTCCTGAGAAAATTTCTGATTCTGAATTAGAAATTCAGATTGAGAAGCTGAAGCAACAAATTTTGAAGTCCTCTTCTGAGCCTCCGAAACTCGATTTAGATGAGGCGATAAAGAAACTGGAAGAGAAACTGGAAAAGGAGGTTGATCAAGAATTGTCAGTGGCCGCTAAAGCCTTGGGCTTAACAGAAAGTTTGTCGAAACTACGTGATGAATTTTCAAAAGCAAGTTCAGATGACCAACCTATTGATCCATTGCTGAAGGGTAAGATAGAAAAACTAAAGGCAGATTTTAATCGAAGGTTGTCAGCAGCTCCTAATGCTAACAAGCTGAAGAAGAAGCATGGCTTGTTGAGAGAATTGACTAAACTGAAGCTTCTCCTGTCAGACAGAAACAAGGAGGCCACAACACTTACGCAGCAACTGAAGAAGAAATTTGATGATGTCATGAATAATCCTAGAATAAAGAAAAACTATGAGGCGTTACGGTCTGAAATCCAGCGTGTTAGCGCATCCTCAGCAAGCGATTTGGATGATGAATTGAAGAAGAAAATCAATGAGTTCAATAAGGAGGTAGACTTGCAGATGGCTAATGTTGTGAAGTCGGTCGGGTTAGATGTTCAGTTAGTAAAACCAGGACGAGATGGCAACGAGTTTTTGGTGCCTCAGATAGAAGAACTAAACAAGGATGTGAAAAAGGAAATTGAAGTTTTGGCAAACTCGTCAACAGATATTAAGAGAATGATGGAGCAATTGAAAGTGGAGGTCGCCAAAGCTGGAGGGACACCAAATTCGGAATCAAAGAATAGAATTGTTGCTTTGACGCAAAAAATAAAGCAGAGCCTTGCAGAGGCCGTAGGCTCTTCTAGTTTGAAAGAGAGATATGAAAACCTGATGTCTGAAACCTCTGGGGATGGAAGTTTGAAAGTAGGAAATGGTCTCACCGATGACGAGTTGAGAGAGAAAGTTGGTGCAAATCGCTCCTTTTCTTAA

Protein sequence

>MS.gene93654.t1

MDELTKMNTEDLLKHRMHKFRKIGGFKEGVPVDPKKKRNMKKRDIPPEKISDSELEIQIEKLKQQILKSSSEPPKLDLDEAIKKLEEKLEKEVDQELSVAAKALGLTESLSKLRDEFSKASSDDQPIDPLLKGKIEKLKADFNRRLSAAPNANKLKKKHGLLRELTKLKLLLSDRNKEATTLTQQLKKKFDDVMNNPRIKKNYEALRSEIQRVSASSASDLDDELKKKINEFNKEVDLQMANVVKSVGLDVQLVKPGRDGNEFLVPQIEELNKDVKKEIEVLANSSTDIKRMMEQLKVEVAKAGGTPNSESKNRIVALTQKIKQSLAEAVGSSSLKERYENLMSETSGDGSLKVGNGLTDDELREKVGANRSFS