Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88243.t1 | XP_013468051.1 | 98.2 | 165 | 3 | 0 | 47 | 211 | 347 | 511 | 4.40E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88243.t1 | Q9LZS0 | 44.3 | 122 | 59 | 3 | 1 | 115 | 302 | 421 | 1.0e-15 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88243.t1 | A0A072VKX9 | 98.2 | 165 | 3 | 0 | 47 | 211 | 347 | 511 | 3.2e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene88243 | 0.802849 | 4.62E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene88243 | 0.845329 | 4.35E-59 | -1.69E-46 |
| MS.gene049341 | MS.gene88243 | 0.817364 | 3.43E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene88243 | 0.822026 | 2.95E-53 | -1.69E-46 |
| MS.gene049435 | MS.gene88243 | 0.827673 | 1.37E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene88243 | 0.839642 | 1.40E-57 | -1.69E-46 |
| MS.gene049821 | MS.gene88243 | 0.838388 | 2.96E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene88243 | 0.827189 | 1.79E-54 | -1.69E-46 |
| MS.gene050020 | MS.gene88243 | 0.879153 | 1.57E-69 | -1.69E-46 |
| MS.gene050137 | MS.gene88243 | 0.809564 | 1.78E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene88243 | 0.820027 | 8.52E-53 | -1.69E-46 |
| MS.gene050438 | MS.gene88243 | 0.807831 | 4.17E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene88243 | 0.823959 | 1.05E-53 | -1.69E-46 |
| MS.gene05045 | MS.gene88243 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene050586 | MS.gene88243 | 0.802524 | 5.39E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene88243 | 0.810205 | 1.29E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene88243 | 0.880648 | 4.62E-70 | -1.69E-46 |
| MS.gene050808 | MS.gene88243 | 0.851457 | 8.76E-61 | -1.69E-46 |
| MS.gene050815 | MS.gene88243 | 0.84759 | 1.05E-59 | -1.69E-46 |
| MS.gene050844 | MS.gene88243 | 0.817026 | 4.08E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene88243 | 0.807255 | 5.53E-50 | -1.69E-46 |
| MS.gene051180 | MS.gene88243 | 0.809843 | 1.55E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene88243 | 0.813648 | 2.30E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene88243 | 0.845943 | 2.96E-59 | -1.69E-46 |
| MS.gene051265 | MS.gene88243 | 0.844794 | 6.06E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene88243 | 0.834438 | 3.00E-56 | -1.69E-46 |
| MS.gene051344 | MS.gene88243 | 0.812377 | 4.37E-51 | -1.69E-46 |
| MS.gene051388 | MS.gene88243 | 0.841072 | 5.93E-58 | -1.69E-46 |
| MS.gene05157 | MS.gene88243 | 0.833458 | 5.27E-56 | -1.69E-46 |
| MS.gene051594 | MS.gene88243 | 0.802932 | 4.44E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene88243 | 0.800392 | 1.47E-48 | -1.69E-46 |
| MS.gene051700 | MS.gene88243 | 0.81168 | 6.20E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene88243 | 0.845393 | 4.18E-59 | -1.69E-46 |
| MS.gene051720 | MS.gene88243 | 0.848443 | 6.11E-60 | -1.69E-46 |
| MS.gene051957 | MS.gene88243 | 0.813776 | 2.16E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene88243 | 0.804525 | 2.07E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene88243 | 0.834507 | 2.88E-56 | -1.69E-46 |
| MS.gene052133 | MS.gene88243 | 0.815029 | 1.14E-51 | -1.69E-46 |
| MS.gene052190 | MS.gene88243 | 0.813841 | 2.09E-51 | -1.69E-46 |
| MS.gene052323 | MS.gene88243 | 0.815031 | 1.14E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene88243 | 0.809403 | 1.93E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene88243 | 0.835277 | 1.84E-56 | -1.69E-46 |
| MS.gene052561 | MS.gene88243 | 0.812346 | 4.44E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene88243 | 0.815951 | 7.11E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene88243 | 0.827862 | 1.24E-54 | -1.69E-46 |
| MS.gene052611 | MS.gene88243 | 0.821984 | 3.02E-53 | -1.69E-46 |
| MS.gene052613 | MS.gene88243 | 0.807335 | 5.32E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene88243 | 0.855391 | 6.51E-62 | -1.69E-46 |
| MS.gene052673 | MS.gene88243 | -0.80132 | 9.52E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene88243 | 0.825791 | 3.87E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene88243 | 0.838643 | 2.55E-57 | -1.69E-46 |
| MS.gene052867 | MS.gene88243 | 0.824222 | 9.07E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene88243 | 0.82251 | 2.28E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene88243 | 0.845367 | 4.24E-59 | -1.69E-46 |
| MS.gene053054 | MS.gene88243 | -0.8054 | 1.36E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene88243 | 0.826724 | 2.32E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene88243 | 0.824045 | 9.98E-54 | -1.69E-46 |
| MS.gene053221 | MS.gene88243 | 0.842897 | 1.95E-58 | -1.69E-46 |
| MS.gene053226 | MS.gene88243 | 0.854293 | 1.36E-61 | -1.69E-46 |
| MS.gene053563 | MS.gene88243 | 0.815467 | 9.11E-52 | -1.69E-46 |
| MS.gene053630 | MS.gene88243 | 0.823522 | 1.32E-53 | -1.69E-46 |
| MS.gene05364 | MS.gene88243 | 0.81032 | 1.22E-50 | -1.69E-46 |
| MS.gene053883 | MS.gene88243 | 0.801564 | 8.48E-49 | -1.69E-46 |
| MS.gene053894 | MS.gene88243 | 0.823256 | 1.53E-53 | -1.69E-46 |
| MS.gene053953 | MS.gene88243 | 0.806782 | 6.96E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene88243 | 0.805981 | 1.03E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene88243 | 0.806909 | 6.54E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene88243 | 0.801124 | 1.04E-48 | -1.69E-46 |
| MS.gene054467 | MS.gene88243 | 0.802594 | 5.21E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene88243 | 0.828316 | 9.62E-55 | -1.69E-46 |
| MS.gene05447 | MS.gene88243 | 0.810907 | 9.13E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene88243 | 0.811256 | 7.67E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene88243 | 0.801448 | 8.96E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene88243 | 0.81329 | 2.76E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene88243 | 0.807473 | 4.97E-50 | -1.69E-46 |
| MS.gene054658 | MS.gene88243 | 0.802286 | 6.03E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene88243 | 0.810276 | 1.25E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene88243 | 0.850248 | 1.92E-60 | -1.69E-46 |
| MS.gene054801 | MS.gene88243 | 0.822681 | 2.08E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene88243 | 0.808627 | 2.82E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene88243 | 0.803304 | 3.72E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene88243 | 0.839258 | 1.76E-57 | -1.69E-46 |
| MS.gene055357 | MS.gene88243 | 0.844987 | 5.38E-59 | -1.69E-46 |
| MS.gene055358 | MS.gene88243 | 0.825679 | 4.11E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene88243 | 0.838557 | 2.68E-57 | -1.69E-46 |
| MS.gene055466 | MS.gene88243 | 0.859959 | 2.88E-63 | -1.69E-46 |
| MS.gene055468 | MS.gene88243 | 0.849936 | 2.35E-60 | -1.69E-46 |
| MS.gene055512 | MS.gene88243 | 0.836191 | 1.08E-56 | -1.69E-46 |
| MS.gene055580 | MS.gene88243 | -0.810976 | 8.82E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene88243 | 0.880917 | 3.70E-70 | -1.69E-46 |
| MS.gene055711 | MS.gene88243 | 0.805528 | 1.28E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene88243 | 0.826307 | 2.91E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene88243 | 0.85242 | 4.67E-61 | -1.69E-46 |
| MS.gene055926 | MS.gene88243 | 0.829139 | 6.08E-55 | -1.69E-46 |
| MS.gene056005 | MS.gene88243 | 0.802473 | 5.52E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene88243 | 0.824591 | 7.43E-54 | -1.69E-46 |
| MS.gene056173 | MS.gene88243 | 0.847765 | 9.40E-60 | -1.69E-46 |
| MS.gene056174 | MS.gene88243 | 0.805584 | 1.24E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene88243 | 0.835722 | 1.42E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene88243 | 0.805113 | 1.56E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene88243 | 0.834535 | 2.83E-56 | -1.69E-46 |
| MS.gene056498 | MS.gene88243 | 0.82847 | 8.83E-55 | -1.69E-46 |
| MS.gene056553 | MS.gene88243 | 0.83936 | 1.66E-57 | -1.69E-46 |
| MS.gene056595 | MS.gene88243 | 0.823029 | 1.73E-53 | -1.69E-46 |
| MS.gene056643 | MS.gene88243 | 0.820324 | 7.28E-53 | -1.69E-46 |
| MS.gene056859 | MS.gene88243 | 0.817712 | 2.86E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene88243 | 0.866624 | 2.49E-65 | -1.69E-46 |
| MS.gene057314 | MS.gene88243 | 0.817973 | 2.50E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene88243 | 0.814016 | 1.91E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene88243 | 0.851654 | 7.71E-61 | -1.69E-46 |
| MS.gene057693 | MS.gene88243 | 0.85126 | 9.96E-61 | -1.69E-46 |
| MS.gene057869 | MS.gene88243 | 0.818829 | 1.60E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene88243 | 0.81328 | 2.77E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene88243 | 0.815699 | 8.09E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene88243 | 0.82772 | 1.34E-54 | -1.69E-46 |
| MS.gene05847 | MS.gene88243 | 0.819213 | 1.31E-52 | -1.69E-46 |
| MS.gene058548 | MS.gene88243 | 0.813056 | 3.10E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene88243 | 0.840923 | 6.49E-58 | -1.69E-46 |
| MS.gene058636 | MS.gene88243 | 0.815544 | 8.75E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene88243 | 0.815694 | 8.11E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene88243 | 0.806129 | 9.56E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene88243 | 0.824096 | 9.71E-54 | -1.69E-46 |
| MS.gene059128 | MS.gene88243 | 0.812325 | 4.49E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene88243 | 0.800037 | 1.74E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene88243 | 0.836372 | 9.73E-57 | -1.69E-46 |
| MS.gene059607 | MS.gene88243 | 0.837818 | 4.15E-57 | -1.69E-46 |
| MS.gene059625 | MS.gene88243 | 0.828018 | 1.14E-54 | -1.69E-46 |
| MS.gene059725 | MS.gene88243 | 0.807842 | 4.15E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene88243 | 0.802719 | 4.91E-49 | -1.69E-46 |
| MS.gene05979 | MS.gene88243 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene88243 | 0.850158 | 2.03E-60 | -1.69E-46 |
| MS.gene059937 | MS.gene88243 | 0.853326 | 2.57E-61 | -1.69E-46 |
| MS.gene060137 | MS.gene88243 | -0.85396 | 1.69E-61 | -1.69E-46 |
| MS.gene060310 | MS.gene88243 | 0.812995 | 3.20E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene88243 | 0.838425 | 2.90E-57 | -1.69E-46 |
| MS.gene060429 | MS.gene88243 | 0.858476 | 8.03E-63 | -1.69E-46 |
| MS.gene060572 | MS.gene88243 | 0.808581 | 2.89E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene88243 | 0.839975 | 1.15E-57 | -1.69E-46 |
| MS.gene060604 | MS.gene88243 | 0.804347 | 2.26E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene88243 | -0.813297 | 2.75E-51 | -1.69E-46 |
| MS.gene060690 | MS.gene88243 | 0.803647 | 3.16E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene88243 | 0.813438 | 2.56E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene88243 | 0.854172 | 1.47E-61 | -1.69E-46 |
| MS.gene060907 | MS.gene88243 | 0.806972 | 6.34E-50 | -1.69E-46 |
| MS.gene060918 | MS.gene88243 | 0.810094 | 1.37E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene88243 | 0.802029 | 6.81E-49 | -1.69E-46 |
| MS.gene061176 | MS.gene88243 | 0.821153 | 4.70E-53 | -1.69E-46 |
| MS.gene061177 | MS.gene88243 | 0.821015 | 5.05E-53 | -1.69E-46 |
| MS.gene061178 | MS.gene88243 | 0.820765 | 5.77E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88243.t1 | MTR_1g060990 | 98.578 | 211 | 3 | 0 | 1 | 211 | 301 | 511 | 5.91e-153 | 434 |
| MS.gene88243.t1 | MTR_1g098900 | 40.000 | 70 | 42 | 0 | 1 | 70 | 288 | 357 | 5.52e-12 | 64.7 |
| MS.gene88243.t1 | MTR_1g492790 | 36.000 | 75 | 47 | 1 | 1 | 74 | 311 | 385 | 6.80e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88243.t1 | AT3G10000 | 37.220 | 223 | 115 | 7 | 1 | 211 | 261 | 470 | 1.65e-31 | 119 |
| MS.gene88243.t1 | AT3G10000 | 37.220 | 223 | 115 | 7 | 1 | 211 | 271 | 480 | 2.22e-31 | 119 |
| MS.gene88243.t1 | AT5G03680 | 47.541 | 122 | 55 | 3 | 1 | 115 | 302 | 421 | 1.13e-25 | 104 |
| MS.gene88243.t1 | AT1G76880 | 42.857 | 70 | 40 | 0 | 1 | 70 | 262 | 331 | 9.30e-11 | 60.8 |
Find 50 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATTTGTGGGAGAATTT+TGG | 0.154763 | 1.2:-46194165 | MS.gene88243:CDS |
| CTCTCATCAGTTAATGGTTT+TGG | 0.213143 | 1.2:+46194121 | None:intergenic |
| ATGGAAGCTTTGAAGAATTT+AGG | 0.224314 | 1.2:-46194558 | MS.gene88243:CDS |
| AGCAAGGGATGCTGCTTTAA+TGG | 0.232544 | 1.2:-46194577 | MS.gene88243:CDS |
| CATGATCATAAGGGTGTAAA+TGG | 0.261143 | 1.2:-46194504 | MS.gene88243:CDS |
| AGGAGGTAGAGAAATGATTA+AGG | 0.310468 | 1.2:-46194538 | MS.gene88243:CDS |
| GTTCTGATAATTGTACTCTA+AGG | 0.329879 | 1.2:-46194413 | MS.gene88243:CDS |
| AAGGGTGTAAATGGAATAAC+TGG | 0.343913 | 1.2:-46194495 | MS.gene88243:CDS |
| TACTCTAAGGGATGATTCTA+AGG | 0.354428 | 1.2:-46194400 | MS.gene88243:CDS |
| TCACCTTCTGATTCTAATGT+TGG | 0.411980 | 1.2:-46194237 | MS.gene88243:CDS |
| TGAAGAAGCTCATGATCATA+AGG | 0.433018 | 1.2:-46194514 | MS.gene88243:CDS |
| ATTCAAAATCAAAATGAAGA+TGG | 0.456037 | 1.2:-46194465 | MS.gene88243:CDS |
| TTTGGAGTAATGAGAGAGCA+TGG | 0.461952 | 1.2:-46194605 | MS.gene88243:CDS |
| ATGATTCTAAGGAAGGAAAC+AGG | 0.472723 | 1.2:-46194389 | MS.gene88243:CDS |
| GGAGGAAACAAGAGGCCAAA+AGG | 0.485530 | 1.2:-46194647 | MS.gene88243:CDS |
| CTTTCCTTTCATGATTAGTG+AGG | 0.500450 | 1.2:-46194190 | MS.gene88243:CDS |
| GACACTTGAGGACAAGGAAA+AGG | 0.501629 | 1.2:-46194694 | MS.gene88243:CDS |
| GGAAGGAAACAGGAAGAGAA+AGG | 0.505443 | 1.2:-46194379 | MS.gene88243:CDS |
| GGGCGTTAAGAGAAGAAGAG+TGG | 0.505945 | 1.2:-46194668 | MS.gene88243:CDS |
| TTCAAAATCAAAATGAAGAT+GGG | 0.510698 | 1.2:-46194464 | MS.gene88243:CDS |
| CAATGTTCTATGTACAATCA+AGG | 0.520932 | 1.2:-46194330 | MS.gene88243:CDS |
| CTAAGGGATGATTCTAAGGA+AGG | 0.524712 | 1.2:-46194396 | MS.gene88243:CDS |
| TAGTGAGGGTGGAAATTTGT+GGG | 0.530887 | 1.2:-46194175 | MS.gene88243:CDS |
| TTTCCTTTCATGATTAGTGA+GGG | 0.536493 | 1.2:-46194189 | MS.gene88243:CDS |
| AAACTACAAAGGAATGATCA+TGG | 0.537652 | 1.2:-46194264 | MS.gene88243:CDS |
| AATGTTGGAAATGTAGCTAA+TGG | 0.561430 | 1.2:-46194222 | MS.gene88243:CDS |
| TTCTGATAATTGTACTCTAA+GGG | 0.561784 | 1.2:-46194412 | MS.gene88243:CDS |
| GAGGAAACAAGAGGCCAAAA+GGG | 0.564460 | 1.2:-46194646 | MS.gene88243:CDS |
| GAAGCTTTGAAGAATTTAGG+AGG | 0.565385 | 1.2:-46194555 | MS.gene88243:CDS |
| CCACCCTCACTAATCATGAA+AGG | 0.571997 | 1.2:+46194186 | None:intergenic |
| CCTTTCATGATTAGTGAGGG+TGG | 0.572090 | 1.2:-46194186 | MS.gene88243:CDS |
| TCTATGTACAATCAAGGAAG+TGG | 0.576269 | 1.2:-46194324 | MS.gene88243:CDS |
| GAGAGCATGGATTGAAGCAA+GGG | 0.577276 | 1.2:-46194592 | MS.gene88243:CDS |
| GAAGAAGCTCATGATCATAA+GGG | 0.582156 | 1.2:-46194513 | MS.gene88243:CDS |
| TTTCCAACATTAGAATCAGA+AGG | 0.583715 | 1.2:+46194234 | None:intergenic |
| AGAGAGCATGGATTGAAGCA+AGG | 0.583741 | 1.2:-46194593 | MS.gene88243:CDS |
| TGTGAAGACACTTGAGGACA+AGG | 0.590043 | 1.2:-46194700 | MS.gene88243:CDS |
| GAAACAAGAGGCCAAAAGGG+TGG | 0.597918 | 1.2:-46194643 | MS.gene88243:CDS |
| TGATGTTAATGATCAAAGAG+AGG | 0.618449 | 1.2:-46194295 | MS.gene88243:CDS |
| CGTTAAGAGAAGAAGAGTGG+AGG | 0.619013 | 1.2:-46194665 | MS.gene88243:CDS |
| ATCTTGAATAGTACTGTGAG+AGG | 0.628009 | 1.2:-46194435 | MS.gene88243:CDS |
| AGAAGAGTGGAGGAAACAAG+AGG | 0.631900 | 1.2:-46194655 | MS.gene88243:CDS |
| TGAGGACAAGGAAAAGGAGA+GGG | 0.634562 | 1.2:-46194688 | MS.gene88243:CDS |
| AGGAGATTGTGAAACTACAA+AGG | 0.636318 | 1.2:-46194275 | MS.gene88243:CDS |
| GAAGCTTGTGGAGAAGCAAG+AGG | 0.641062 | 1.2:-46194739 | MS.gene88243:CDS |
| TGCACAAATGAAGAAGCTTG+TGG | 0.649032 | 1.2:-46194751 | None:intergenic |
| TTGTGGAGAAGCAAGAGGAG+TGG | 0.656273 | 1.2:-46194734 | MS.gene88243:CDS |
| TTGAGGACAAGGAAAAGGAG+AGG | 0.672502 | 1.2:-46194689 | MS.gene88243:CDS |
| TTAGTGAGGGTGGAAATTTG+TGG | 0.685166 | 1.2:-46194176 | MS.gene88243:CDS |
| TAAGCTTGTGAAGACACTTG+AGG | 0.695179 | 1.2:-46194706 | MS.gene88243:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAAATCAAAATGAAGA+TGG | - | chr1.2:46194410-46194429 | MS.gene88243:CDS | 20.0% |
| !! | TTCAAAATCAAAATGAAGAT+GGG | - | chr1.2:46194411-46194430 | MS.gene88243:CDS | 20.0% |
| ! | TTCTGATAATTGTACTCTAA+GGG | - | chr1.2:46194463-46194482 | MS.gene88243:CDS | 25.0% |
| !! | TGGAAAAAGAACAAAAGTTT+TGG | - | chr1.2:46194252-46194271 | MS.gene88243:CDS | 25.0% |
| AAACTACAAAGGAATGATCA+TGG | - | chr1.2:46194611-46194630 | MS.gene88243:CDS | 30.0% | |
| AATGTTGGAAATGTAGCTAA+TGG | - | chr1.2:46194653-46194672 | MS.gene88243:CDS | 30.0% | |
| CAATGTTCTATGTACAATCA+AGG | - | chr1.2:46194545-46194564 | MS.gene88243:CDS | 30.0% | |
| GTTCTGATAATTGTACTCTA+AGG | - | chr1.2:46194462-46194481 | MS.gene88243:CDS | 30.0% | |
| TGATGTTAATGATCAAAGAG+AGG | - | chr1.2:46194580-46194599 | MS.gene88243:CDS | 30.0% | |
| TTTCCAACATTAGAATCAGA+AGG | + | chr1.2:46194644-46194663 | None:intergenic | 30.0% | |
| TTTCCTTTCATGATTAGTGA+GGG | - | chr1.2:46194686-46194705 | MS.gene88243:CDS | 30.0% | |
| !! | ATGGAAGCTTTGAAGAATTT+AGG | - | chr1.2:46194317-46194336 | MS.gene88243:CDS | 30.0% |
| AGGAGATTGTGAAACTACAA+AGG | - | chr1.2:46194600-46194619 | MS.gene88243:CDS | 35.0% | |
| AGGAGGTAGAGAAATGATTA+AGG | - | chr1.2:46194337-46194356 | MS.gene88243:CDS | 35.0% | |
| ATCTTGAATAGTACTGTGAG+AGG | - | chr1.2:46194440-46194459 | MS.gene88243:CDS | 35.0% | |
| CTTTCCTTTCATGATTAGTG+AGG | - | chr1.2:46194685-46194704 | MS.gene88243:CDS | 35.0% | |
| GAAGAAGCTCATGATCATAA+GGG | - | chr1.2:46194362-46194381 | MS.gene88243:CDS | 35.0% | |
| TCACCTTCTGATTCTAATGT+TGG | - | chr1.2:46194638-46194657 | MS.gene88243:CDS | 35.0% | |
| TCTATGTACAATCAAGGAAG+TGG | - | chr1.2:46194551-46194570 | MS.gene88243:CDS | 35.0% | |
| TGAAGAAGCTCATGATCATA+AGG | - | chr1.2:46194361-46194380 | MS.gene88243:CDS | 35.0% | |
| ! | AAGGGTGTAAATGGAATAAC+TGG | - | chr1.2:46194380-46194399 | MS.gene88243:CDS | 35.0% |
| ! | ATGATTCTAAGGAAGGAAAC+AGG | - | chr1.2:46194486-46194505 | MS.gene88243:CDS | 35.0% |
| ! | CATGATCATAAGGGTGTAAA+TGG | - | chr1.2:46194371-46194390 | MS.gene88243:CDS | 35.0% |
| ! | GGAAATTTGTGGGAGAATTT+TGG | - | chr1.2:46194710-46194729 | MS.gene88243:CDS | 35.0% |
| ! | TACTCTAAGGGATGATTCTA+AGG | - | chr1.2:46194475-46194494 | MS.gene88243:CDS | 35.0% |
| ! | TTGTTCTTTTTCCACCCTTT+TGG | + | chr1.2:46194246-46194265 | None:intergenic | 35.0% |
| !! | GAAGCTTTGAAGAATTTAGG+AGG | - | chr1.2:46194320-46194339 | MS.gene88243:CDS | 35.0% |
| TAAGCTTGTGAAGACACTTG+AGG | - | chr1.2:46194169-46194188 | MS.gene88243:CDS | 40.0% | |
| TAGTGAGGGTGGAAATTTGT+GGG | - | chr1.2:46194700-46194719 | MS.gene88243:CDS | 40.0% | |
| TTAGTGAGGGTGGAAATTTG+TGG | - | chr1.2:46194699-46194718 | MS.gene88243:CDS | 40.0% | |
| ! | CTAAGGGATGATTCTAAGGA+AGG | - | chr1.2:46194479-46194498 | MS.gene88243:CDS | 40.0% |
| ! | TTTGGAGTAATGAGAGAGCA+TGG | - | chr1.2:46194270-46194289 | MS.gene88243:CDS | 40.0% |
| AGAAGAGTGGAGGAAACAAG+AGG | - | chr1.2:46194220-46194239 | MS.gene88243:CDS | 45.0% | |
| CCACCCTCACTAATCATGAA+AGG | + | chr1.2:46194692-46194711 | None:intergenic | 45.0% | |
| CCTTTCATGATTAGTGAGGG+TGG | - | chr1.2:46194689-46194708 | MS.gene88243:CDS | 45.0% | |
| CGTTAAGAGAAGAAGAGTGG+AGG | - | chr1.2:46194210-46194229 | MS.gene88243:CDS | 45.0% | |
| GACACTTGAGGACAAGGAAA+AGG | - | chr1.2:46194181-46194200 | MS.gene88243:CDS | 45.0% | |
| GAGGAAACAAGAGGCCAAAA+GGG | - | chr1.2:46194229-46194248 | MS.gene88243:CDS | 45.0% | |
| GGAAGGAAACAGGAAGAGAA+AGG | - | chr1.2:46194496-46194515 | MS.gene88243:CDS | 45.0% | |
| TGAGGACAAGGAAAAGGAGA+GGG | - | chr1.2:46194187-46194206 | MS.gene88243:CDS | 45.0% | |
| TGTGAAGACACTTGAGGACA+AGG | - | chr1.2:46194175-46194194 | MS.gene88243:CDS | 45.0% | |
| TTGAGGACAAGGAAAAGGAG+AGG | - | chr1.2:46194186-46194205 | MS.gene88243:CDS | 45.0% | |
| ! | AGAGAGCATGGATTGAAGCA+AGG | - | chr1.2:46194282-46194301 | MS.gene88243:CDS | 45.0% |
| ! | GAGAGCATGGATTGAAGCAA+GGG | - | chr1.2:46194283-46194302 | MS.gene88243:CDS | 45.0% |
| !! | AGCAAGGGATGCTGCTTTAA+TGG | - | chr1.2:46194298-46194317 | MS.gene88243:CDS | 45.0% |
| GAAACAAGAGGCCAAAAGGG+TGG | - | chr1.2:46194232-46194251 | MS.gene88243:CDS | 50.0% | |
| GAAGCTTGTGGAGAAGCAAG+AGG | - | chr1.2:46194136-46194155 | MS.gene88243:CDS | 50.0% | |
| GGAGGAAACAAGAGGCCAAA+AGG | - | chr1.2:46194228-46194247 | MS.gene88243:CDS | 50.0% | |
| GGGCGTTAAGAGAAGAAGAG+TGG | - | chr1.2:46194207-46194226 | MS.gene88243:CDS | 50.0% | |
| TTGTGGAGAAGCAAGAGGAG+TGG | - | chr1.2:46194141-46194160 | MS.gene88243:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 46194131 | 46194766 | 46194131 | ID=MS.gene88243 |
| chr1.2 | mRNA | 46194131 | 46194766 | 46194131 | ID=MS.gene88243.t1;Parent=MS.gene88243 |
| chr1.2 | exon | 46194131 | 46194766 | 46194131 | ID=MS.gene88243.t1.exon1;Parent=MS.gene88243.t1 |
| chr1.2 | CDS | 46194131 | 46194766 | 46194131 | ID=cds.MS.gene88243.t1;Parent=MS.gene88243.t1 |
| Gene Sequence |
| Protein sequence |
