AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53586


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53586.t1 MTR_1g046070 96.996 233 5 1 1 231 1 233 8.00e-155 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53586.t1 AT4G13540 46.061 165 71 4 49 212 37 184 2.25e-32 117
MS.gene53586.t1 AT3G23930 41.279 172 78 4 52 212 40 199 1.88e-23 94.4

Find 49 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAAAGAGTGGGAATTAA+TGG 0.247721 1.2:-28866911 MS.gene53586:CDS
ACTTACCATCATATGTTCTT+TGG 0.249382 1.2:+28866776 None:intergenic
GAAGAAGTTGAAAATCAAAT+TGG 0.250274 1.2:-28865936 MS.gene53586:CDS
TTCTTCTCTTTAGTTTGAAT+TGG 0.254934 1.2:+28865956 None:intergenic
AAAGCTTTAAAAGCTCAATT+AGG 0.297525 1.2:-28866064 MS.gene53586:CDS
ATTAATGGGGACTAAGCTTT+TGG 0.315398 1.2:-28866896 MS.gene53586:CDS
AAAGGGAATTTGACTTGTTA+AGG 0.331176 1.2:-28866012 MS.gene53586:CDS
TAAAGGAGATAATGTGTGAT+AGG 0.355573 1.2:-28867110 MS.gene53586:CDS
ACTTCTACAAGAAGAAATAA+AGG 0.374088 1.2:-28867127 MS.gene53586:CDS
GAACTATGAGAGGGAGATAA+TGG 0.387809 1.2:-28867073 MS.gene53586:CDS
GAGAAAGAGTGGGAATTAAT+GGG 0.405188 1.2:-28866910 MS.gene53586:CDS
GAGGGAAGTGGTGGAAGAGA+AGG 0.429388 1.2:-28866983 MS.gene53586:CDS
AAGAGAAGGAAGAGAAGATA+AGG 0.430927 1.2:-28866969 MS.gene53586:CDS
AAGAAGTTGAAAATCAAATT+GGG 0.435875 1.2:-28865935 MS.gene53586:CDS
GGTGAAGCAGTTGAGGGAAG+TGG 0.438865 1.2:-28866995 MS.gene53586:CDS
AAGAAGAGGTGAAGCAGTTG+AGG 0.440787 1.2:-28867002 MS.gene53586:CDS
TCACAGGAGATGGTTTGTAT+TGG 0.451338 1.2:-28866156 MS.gene53586:intron
AGAAGAATTGTGAGAAAGAG+TGG 0.454742 1.2:-28866921 MS.gene53586:CDS
ATAAGGGAGATGGAAGAGGT+TGG 0.460472 1.2:-28866952 MS.gene53586:CDS
AAGAAAGGTACAAACAAGAA+AGG 0.469395 1.2:-28866030 MS.gene53586:CDS
AGAGAAGGAAGAGAAGATAA+GGG 0.476340 1.2:-28866968 MS.gene53586:CDS
AGAAGAGGTGAAGCAGTTGA+GGG 0.480459 1.2:-28867001 MS.gene53586:CDS
AAGAGAGTAGGAACTATGAG+AGG 0.487797 1.2:-28867083 MS.gene53586:CDS
GAAGATAAGGGAGATGGAAG+AGG 0.488344 1.2:-28866956 MS.gene53586:CDS
TACAAATTGAAAATCTGAAG+AGG 0.492525 1.2:-28866114 MS.gene53586:CDS
GTGATAGGGAGAAAGAGAGT+AGG 0.501313 1.2:-28867095 MS.gene53586:CDS
GGAAGAGAAGATAAGGGAGA+TGG 0.503969 1.2:-28866962 MS.gene53586:CDS
TCTTGCAATTAAGAATGAGT+TGG 0.505081 1.2:-28866809 MS.gene53586:CDS
GAGATGAAGCTGTTGAGAAG+TGG 0.508742 1.2:-28866843 MS.gene53586:CDS
TAGGTTCAGTGGAGAAAGAA+AGG 0.512086 1.2:-28866045 MS.gene53586:CDS
GAAGCAGTTGAGGGAAGTGG+TGG 0.513840 1.2:-28866992 MS.gene53586:CDS
AAAAGCTCAATTAGGTTCAG+TGG 0.518823 1.2:-28866056 MS.gene53586:CDS
AGAGAGTAGGAACTATGAGA+GGG 0.522297 1.2:-28867082 MS.gene53586:CDS
CTCATCCAAAGAACATATGA+TGG 0.522947 1.2:-28866781 MS.gene53586:intron
AAAGGAGATAATGTGTGATA+GGG 0.526845 1.2:-28867109 MS.gene53586:CDS
AGAAAGGTACAAACAAGAAA+GGG 0.530283 1.2:-28866029 MS.gene53586:CDS
GGAGATGGAAGAGGTTGGAA+TGG 0.546010 1.2:-28866947 MS.gene53586:CDS
ACTTGTTAAGGCAAAGCTTG+AGG 0.554263 1.2:-28866000 MS.gene53586:CDS
CAAAGCTTGAGGATCATGAA+TGG 0.565818 1.2:-28865989 MS.gene53586:CDS
GGTGAAGAGGTTGAAAGAAG+AGG 0.569093 1.2:-28867016 MS.gene53586:CDS
ATTGGAAACAAGAGGTGAAG+AGG 0.596605 1.2:-28867029 MS.gene53586:CDS
ACAAATTGAAAATCTGAAGA+GGG 0.598118 1.2:-28866113 MS.gene53586:CDS
GAAGAATTGTGAGAAAGAGT+GGG 0.599083 1.2:-28866920 MS.gene53586:CDS
AGAAAGAGTGGGAATTAATG+GGG 0.618319 1.2:-28866909 MS.gene53586:CDS
AGATGAAGGAGGAAAGAGCA+AGG 0.618929 1.2:-28866867 MS.gene53586:CDS
TGGTTTGTATTGGAAAGCTG+AGG 0.636251 1.2:-28866146 MS.gene53586:CDS
GGAGGCAGATTGGAAACAAG+AGG 0.643296 1.2:-28867037 MS.gene53586:CDS
TTTGGTTCAAGAGATGAAGG+AGG 0.645416 1.2:-28866878 MS.gene53586:CDS
GGGGCCAATTCAAAGATGGG+GGG 0.731569 1.2:-28867225 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACTATAAACTGTTTTTAT+AGG + chr1.2:28866624-28866643 None:intergenic 15.0%
!!! AAATTCATTACATATTTTTG+TGG + chr1.2:28865976-28865995 None:intergenic 15.0%
!! AAATACAAACACTAATTGTT+TGG + chr1.2:28866738-28866757 None:intergenic 20.0%
!! AAGAAGTTGAAAATCAAATT+GGG - chr1.2:28867203-28867222 MS.gene53586:CDS 20.0%
!! ATTAATATGTGACAATAATG+TGG - chr1.2:28866863-28866882 MS.gene53586:CDS 20.0%
!!! AATTTTGAAAAGTACTATGA+TGG + chr1.2:28866838-28866857 None:intergenic 20.0%
!!! ATTTTGAAAAGTACTATGAT+GGG + chr1.2:28866837-28866856 None:intergenic 20.0%
! ACAAATTGAAAATCTGAAGA+GGG - chr1.2:28867025-28867044 MS.gene53586:CDS 25.0%
! ACTTCTACAAGAAGAAATAA+AGG - chr1.2:28866011-28866030 MS.gene53586:CDS 25.0%
! GAAGAAGTTGAAAATCAAAT+TGG - chr1.2:28867202-28867221 MS.gene53586:CDS 25.0%
! TACAAATTGAAAATCTGAAG+AGG - chr1.2:28867024-28867043 MS.gene53586:CDS 25.0%
!!! AAAGCTTTAAAAGCTCAATT+AGG - chr1.2:28867074-28867093 MS.gene53586:CDS 25.0%
!!! TTCTTCTCTTTAGTTTGAAT+TGG + chr1.2:28867185-28867204 None:intergenic 25.0%
AAAGGAGATAATGTGTGATA+GGG - chr1.2:28866029-28866048 MS.gene53586:CDS 30.0%
ACTTACCATCATATGTTCTT+TGG + chr1.2:28866365-28866384 None:intergenic 30.0%
TAAAGGAGATAATGTGTGAT+AGG - chr1.2:28866028-28866047 MS.gene53586:CDS 30.0%
TCTTGCAATTAAGAATGAGT+TGG - chr1.2:28866329-28866348 MS.gene53586:intron 30.0%
! AAGAAAGGTACAAACAAGAA+AGG - chr1.2:28867108-28867127 MS.gene53586:CDS 30.0%
! AGAAAGGTACAAACAAGAAA+GGG - chr1.2:28867109-28867128 MS.gene53586:CDS 30.0%
! TACTTGAGCTTATTTGCTTT+TGG - chr1.2:28866951-28866970 MS.gene53586:CDS 30.0%
!! AAAGGGAATTTGACTTGTTA+AGG - chr1.2:28867126-28867145 MS.gene53586:CDS 30.0%
!! GATAATGGTGTTTGCTTTTA+AGG - chr1.2:28866080-28866099 MS.gene53586:CDS 30.0%
AAAAGCTCAATTAGGTTCAG+TGG - chr1.2:28867082-28867101 MS.gene53586:CDS 35.0%
AAGAGAAGGAAGAGAAGATA+AGG - chr1.2:28866169-28866188 MS.gene53586:intron 35.0%
AGAAAGAGTGGGAATTAATG+GGG - chr1.2:28866229-28866248 MS.gene53586:intron 35.0%
AGAAGAATTGTGAGAAAGAG+TGG - chr1.2:28866217-28866236 MS.gene53586:intron 35.0%
AGAGAAGGAAGAGAAGATAA+GGG - chr1.2:28866170-28866189 MS.gene53586:intron 35.0%
CTCATCCAAAGAACATATGA+TGG - chr1.2:28866357-28866376 MS.gene53586:intron 35.0%
GAAGAATTGTGAGAAAGAGT+GGG - chr1.2:28866218-28866237 MS.gene53586:intron 35.0%
GAGAAAGAGTGGGAATTAAT+GGG - chr1.2:28866228-28866247 MS.gene53586:intron 35.0%
TGAGAAAGAGTGGGAATTAA+TGG - chr1.2:28866227-28866246 MS.gene53586:intron 35.0%
! ATTAATGGGGACTAAGCTTT+TGG - chr1.2:28866242-28866261 MS.gene53586:intron 35.0%
!! AATGGTGTTTGCTTTTAAGG+AGG - chr1.2:28866083-28866102 MS.gene53586:CDS 35.0%
!! GCTTTTGGAAATATACTCAC+AGG - chr1.2:28866966-28866985 MS.gene53586:CDS 35.0%
AAAAGTAGTGTGTGGCAGAT+GGG - chr1.2:28866804-28866823 MS.gene53586:CDS 40.0%
AAGAGAGTAGGAACTATGAG+AGG - chr1.2:28866055-28866074 MS.gene53586:CDS 40.0%
AGAGAGTAGGAACTATGAGA+GGG - chr1.2:28866056-28866075 MS.gene53586:CDS 40.0%
ATTGGAAACAAGAGGTGAAG+AGG - chr1.2:28866109-28866128 MS.gene53586:CDS 40.0%
CAAAGCTTGAGGATCATGAA+TGG - chr1.2:28867149-28867168 MS.gene53586:CDS 40.0%
GAACTATGAGAGGGAGATAA+TGG - chr1.2:28866065-28866084 MS.gene53586:CDS 40.0%
GGAAATATACTCACAGGAGA+TGG - chr1.2:28866972-28866991 MS.gene53586:CDS 40.0%
TAGGTTCAGTGGAGAAAGAA+AGG - chr1.2:28867093-28867112 MS.gene53586:CDS 40.0%
! ACTTGTTAAGGCAAAGCTTG+AGG - chr1.2:28867138-28867157 MS.gene53586:CDS 40.0%
! TCACAGGAGATGGTTTGTAT+TGG - chr1.2:28866982-28867001 MS.gene53586:CDS 40.0%
! TGGTTTGTATTGGAAAGCTG+AGG - chr1.2:28866992-28867011 MS.gene53586:CDS 40.0%
! TTTGGTTCAAGAGATGAAGG+AGG - chr1.2:28866260-28866279 MS.gene53586:intron 40.0%
!! GCCACACACTACTTTTCAAA+GGG + chr1.2:28866800-28866819 None:intergenic 40.0%
!! TGCCACACACTACTTTTCAA+AGG + chr1.2:28866801-28866820 None:intergenic 40.0%
!! TTGCTTTTAAGGAGGCAGAT+TGG - chr1.2:28866091-28866110 MS.gene53586:CDS 40.0%
!!! GCTTTTGGTTCAAGAGATGA+AGG - chr1.2:28866257-28866276 MS.gene53586:intron 40.0%
AAAGTAGTGTGTGGCAGATG+GGG - chr1.2:28866805-28866824 MS.gene53586:CDS 45.0%
AAGAAGAGGTGAAGCAGTTG+AGG - chr1.2:28866136-28866155 MS.gene53586:CDS 45.0%
AGAAGAGGTGAAGCAGTTGA+GGG - chr1.2:28866137-28866156 MS.gene53586:CDS 45.0%
AGATGAAGGAGGAAAGAGCA+AGG - chr1.2:28866271-28866290 MS.gene53586:intron 45.0%
ATAAGGGAGATGGAAGAGGT+TGG - chr1.2:28866186-28866205 MS.gene53586:intron 45.0%
GAAAAGTAGTGTGTGGCAGA+TGG - chr1.2:28866803-28866822 MS.gene53586:CDS 45.0%
GAAGATAAGGGAGATGGAAG+AGG - chr1.2:28866182-28866201 MS.gene53586:intron 45.0%
GAGATGAAGCTGTTGAGAAG+TGG - chr1.2:28866295-28866314 MS.gene53586:intron 45.0%
GGAAGAGAAGATAAGGGAGA+TGG - chr1.2:28866176-28866195 MS.gene53586:intron 45.0%
GGTGAAGAGGTTGAAAGAAG+AGG - chr1.2:28866122-28866141 MS.gene53586:CDS 45.0%
GTGATAGGGAGAAAGAGAGT+AGG - chr1.2:28866043-28866062 MS.gene53586:CDS 45.0%
! GCCCTTTGAAAAGTAGTGTG+TGG - chr1.2:28866796-28866815 MS.gene53586:CDS 45.0%
!!! AAAATTAAATAAAATTCTTT+TGG + chr1.2:28866650-28866669 None:intergenic 5.0%
GGAGATGGAAGAGGTTGGAA+TGG - chr1.2:28866191-28866210 MS.gene53586:intron 50.0%
GGAGGCAGATTGGAAACAAG+AGG - chr1.2:28866101-28866120 MS.gene53586:CDS 50.0%
GAAGCAGTTGAGGGAAGTGG+TGG - chr1.2:28866146-28866165 MS.gene53586:CDS 55.0%
GGTGAAGCAGTTGAGGGAAG+TGG - chr1.2:28866143-28866162 MS.gene53586:CDS 55.0%
! GAGGGAAGTGGTGGAAGAGA+AGG - chr1.2:28866155-28866174 MS.gene53586:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 28865928 28867232 28865928 ID=MS.gene53586
chr1.2 mRNA 28865928 28867232 28865928 ID=MS.gene53586.t1;Parent=MS.gene53586
chr1.2 exon 28866782 28867232 28866782 ID=MS.gene53586.t1.exon1;Parent=MS.gene53586.t1
chr1.2 CDS 28866782 28867232 28866782 ID=cds.MS.gene53586.t1;Parent=MS.gene53586.t1
chr1.2 exon 28865928 28866172 28865928 ID=MS.gene53586.t1.exon2;Parent=MS.gene53586.t1
chr1.2 CDS 28865928 28866172 28865928 ID=cds.MS.gene53586.t1;Parent=MS.gene53586.t1
Gene Sequence

>MS.gene53586

ATGGGGGGAAGTGTTAGTGTTAGCAAGAAAAATGAGATAAAGTATCCACAAAAATATGTAATGAATTTGAAAGAGAAAGTGAGACTTCTACAAGAAGAAATAAAGGAGATAATGTGTGATAGGGAGAAAGAGAGTAGGAACTATGAGAGGGAGATAATGGTGTTTGCTTTTAAGGAGGCAGATTGGAAACAAGAGGTGAAGAGGTTGAAAGAAGAGGTGAAGCAGTTGAGGGAAGTGGTGGAAGAGAAGGAAGAGAAGATAAGGGAGATGGAAGAGGTTGGAATGGTTGAGAAGAATTGTGAGAAAGAGTGGGAATTAATGGGGACTAAGCTTTTGGTTCAAGAGATGAAGGAGGAAAGAGCAAGGAGAGATGAAGCTGTTGAGAAGTGGAAACAACTTTATCTTGCAATTAAGAATGAGTTGGATGATCTCATCCAAAGAACATATGATGGTAAGTTAATCATAATTTGTTATTAATGTCTTAAGACATGTTTGAATTGAGTTACTTTAGATTATTTATGAGCATCTTTGAGAGTTTATAAAAACAAATTATAATGTGTATAATCTATCTATTTTTATCTTATAAGTTGAATTGAGTTATTTTAGCTTATTTATGAGCATGTTTGAGAGAGTTTATAAAAAACAAATTATAATGTGTGTAATCTATTTTTATCTTATAGTTTTCTAAAATAGCCTATAAAAACAGTTTATAGTTTATGCCAAAAGAATTTTATTTAATTTTTTTTTGTTGTAGAAATAGCTTATAAAATAATGATTATATAATAATTGTTTAATTAAGATTTTTATCCAAACAATTAGTGTTTGTATTTTTTTTTGTGATTTGCATTAGTCTTTGTATGCTATTAATGCCCTTTGAAAAGTAGTGTGTGGCAGATGGGGTTCAATCCCATCATAGTACTTTTCAAAATTAGTCAATTAATATGTGACAATAATGTGGTGAATTATTTTTATTATCTTCTTGAGAAATATTCATACTACTATGCATGAATGCTATGTTTGAACTACTTGAGCTTATTTGCTTTTGGAAATATACTCACAGGAGATGGTTTGTATTGGAAAGCTGAGGAAAATGACATACAAATTGAAAATCTGAAGAGGGAATTGCAAGAGAAAGAAGAAAGCATAAAAGCTTTAAAAGCTCAATTAGGTTCAGTGGAGAAAGAAAGGTACAAACAAGAAAGGGAATTTGACTTGTTAAGGCAAAGCTTGAGGATCATGAATGGCAAGAAGAATCCAATTCAAACTAAAGAGAAGAAGAAGTTGAAAATCAAATTGGGAAAATAA

Protein sequence

>MS.gene53586.t1

MGGSVSVSKKNEIKYPQKYVMNLKEKVRLLQEEIKEIMCDREKESRNYEREIMVFAFKEADWKQEVKRLKEEVKQLREVVEEKEEKIREMEEVGMVEKNCEKEWELMGTKLLVQEMKEERARRDEAVEKWKQLYLAIKNELDDLIQRTYDGDGLYWKAEENDIQIENLKRELQEKEESIKALKAQLGSVEKERYKQEREFDLLRQSLRIMNGKKNPIQTKEKKKLKIKLGK