AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063080.t1 MTR_3g118210 99.558 226 1 0 1 226 47 272 1.32e-164 455
MS.gene063080.t1 MTR_6g006970 43.478 207 111 6 12 216 49 251 6.20e-47 157
MS.gene063080.t1 MTR_1g093060 41.546 207 115 6 12 216 102 304 5.44e-43 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063080.t1 AT2G43030 83.186 226 38 0 1 226 46 271 1.40e-130 369
MS.gene063080.t1 AT3G17465 37.037 216 133 3 11 225 77 290 1.65e-38 136

Find 80 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATCTTAAGCTTTC+TGG 0.097361 3.3:-95911109 None:intergenic
AAAGAGACTAATTAATTCTT+TGG 0.209215 3.3:-95911234 None:intergenic
AAAGCTCATCATTCCCAATT+TGG 0.228676 3.3:-95910519 None:intergenic
CAAATTGACCAAACCTGAAA+TGG 0.282604 3.3:+95910676 MS.gene063080:CDS
CATTCCTATGCGCCATCTTC+AGG 0.308173 3.3:+95910721 MS.gene063080:CDS
TTTCGTCTTCATTCCGTTGA+TGG 0.308819 3.3:+95910746 MS.gene063080:CDS
ATGATCAAAGGTGCTCTTCC+TGG 0.365904 3.3:+95911156 MS.gene063080:CDS
TCAAATACAAGGCGCTGATT+AGG 0.398930 3.3:-95910776 None:intergenic
AAGATGACCCATTTCAGGTT+TGG 0.399688 3.3:-95910684 None:intergenic
CTCTTGGAACCATGGCTCAT+TGG 0.403785 3.3:-95910988 None:intergenic
TGGTGTGATGGGTACCAAAT+TGG 0.414953 3.3:+95910505 MS.gene063080:CDS
GTTGTTCCCGTCACTGTTGT+CGG 0.415769 3.3:+95910560 MS.gene063080:CDS
GGTGATCTTGTTGATGTCTC+TGG 0.428399 3.3:+95910815 MS.gene063080:CDS
AAGCTTTCTGGTCTTCCTGT+TGG 0.434373 3.3:-95911097 None:intergenic
ACGCCGTCCAAGTTGGTTAC+CGG 0.438083 3.3:+95910639 MS.gene063080:CDS
TCCAAGTTGTCTGTGACTCT+TGG 0.442433 3.3:-95911004 None:intergenic
TCCAAGAGTCACAGACAACT+TGG 0.460086 3.3:+95911003 MS.gene063080:CDS
TTCTTGCCAACAATCTTAGC+GGG 0.460342 3.3:-95911207 None:intergenic
ACTGTTGTCGGTTTCAAAGA+AGG 0.461503 3.3:+95910572 MS.gene063080:CDS
AGAGGCTGGTGTTGGTGTGA+TGG 0.463975 3.3:+95910493 MS.gene063080:CDS
TACACCTGGTCGCGTATACA+AGG 0.469173 3.3:+95911044 MS.gene063080:CDS
TTTCAGGTTTGGTCAATTTG+CGG 0.469193 3.3:-95910673 None:intergenic
GTTGTAACCGTCGGTGGCGT+CGG 0.473671 3.3:-95910618 None:intergenic
AAAGAAGATGCCTGGTAGGA+TGG 0.474224 3.3:+95911068 MS.gene063080:CDS
GAGGCTGGTGTTGGTGTGAT+GGG 0.474712 3.3:+95910494 MS.gene063080:CDS
TAACCCTCCGGTAACCAACT+TGG 0.478957 3.3:-95910646 None:intergenic
CACAGACAACTTGGATCCAT+TGG 0.481197 3.3:+95911012 MS.gene063080:CDS
GTAGGATGGGTGGAACCAAC+AGG 0.493432 3.3:+95911082 MS.gene063080:CDS
TACGCGACCAGGTGTAGTTC+CGG 0.494636 3.3:-95911037 None:intergenic
TTTCCCTTGTATACGCGACC+AGG 0.499382 3.3:-95911048 None:intergenic
AGAAGATTACCAGGCTTACC+AGG 0.500896 3.3:-95911174 None:intergenic
ACGGGAACAACTTCACCGTT+AGG 0.508452 3.3:-95910548 None:intergenic
AAGAAGATGCCTGGTAGGAT+GGG 0.509948 3.3:+95911069 MS.gene063080:CDS
GGTGTGATGGGTACCAAATT+GGG 0.512034 3.3:+95910506 MS.gene063080:CDS
AAATTGACCAAACCTGAAAT+GGG 0.513032 3.3:+95910677 MS.gene063080:CDS
TTGAAACCGACAACAGTGAC+GGG 0.513527 3.3:-95910566 None:intergenic
GGTGCTCTTCCTGGTAAGCC+TGG 0.514459 3.3:+95911165 MS.gene063080:CDS
TGAAATGGGTCATCTTGAAA+AGG 0.516033 3.3:+95910691 MS.gene063080:CDS
AAGCGTCATAACTTCAAAAG+AGG 0.517261 3.3:+95910964 MS.gene063080:CDS
AGGTGTAGTTCCGGCACCAA+TGG 0.518535 3.3:-95911028 None:intergenic
TTGGTTCCACCCATCCTACC+AGG 0.519178 3.3:-95911078 None:intergenic
GATGTCTCTGGCACCACCAT+CGG 0.522268 3.3:+95910827 MS.gene063080:CDS
GGTGGTGAAAATGAGCTTAG+AGG 0.522460 3.3:+95910475 MS.gene063080:CDS
CTGAAGATGGCGCATAGGAA+TGG 0.527595 3.3:-95910720 None:intergenic
ATGGCGCATAGGAATGGCTC+CGG 0.528063 3.3:-95910714 None:intergenic
GTAAGCCTGGTAATCTTCTA+CGG 0.530779 3.3:+95911178 MS.gene063080:CDS
GAACAACTTCACCGTTAGGT+TGG 0.533148 3.3:-95910544 None:intergenic
CTCTGGCACCACCATCGGCA+AGG 0.534330 3.3:+95910832 MS.gene063080:CDS
TACAAGGGAAAGAAGATGCC+TGG 0.536322 3.3:+95911060 MS.gene063080:CDS
GTCTGTGACTCTTGGAACCA+TGG 0.543733 3.3:-95910996 None:intergenic
GAAGACGAAACTCCTGAAGA+TGG 0.564919 3.3:-95910733 None:intergenic
ATGGGTCATCTTGAAAAGGC+CGG 0.565417 3.3:+95910695 MS.gene063080:CDS
CAACTTGGATCCATTGGTGC+CGG 0.569215 3.3:+95911018 MS.gene063080:CDS
ATTTGCGGTCTCTAACCCTC+CGG 0.569415 3.3:-95910658 None:intergenic
TCTGGCACCACCATCGGCAA+GGG 0.572275 3.3:+95910833 MS.gene063080:CDS
CTTAATGTTATCATGATCAA+AGG 0.572918 3.3:+95911144 MS.gene063080:CDS
ATTGGTGCCGGAACTACACC+TGG 0.580799 3.3:+95911030 MS.gene063080:CDS
TTTGAAACCGACAACAGTGA+CGG 0.586413 3.3:-95910567 None:intergenic
CCGTCCAAGTTGGTTACCGG+AGG 0.591201 3.3:+95910642 MS.gene063080:CDS
GTTCTTGCCAACAATCTTAG+CGG 0.594511 3.3:-95911208 None:intergenic
AAGATGCCTGGTAGGATGGG+TGG 0.594546 3.3:+95911072 MS.gene063080:CDS
ATTAGGTTCAAAACCATCAA+CGG 0.594549 3.3:-95910759 None:intergenic
ACCTTGAAAACCCTTGCCGA+TGG 0.600155 3.3:-95910843 None:intergenic
TGAAAAGCTCATCAAATACA+AGG 0.600821 3.3:-95910787 None:intergenic
GCTATCCGTAGAAGATTACC+AGG 0.602366 3.3:-95911183 None:intergenic
AGGGAAAGAAGATGCCTGGT+AGG 0.605040 3.3:+95911064 MS.gene063080:CDS
TTGAAAACCCTTGCCGATGG+TGG 0.607518 3.3:-95910840 None:intergenic
ATCAAAACCGACGCCACCGA+CGG 0.609949 3.3:+95910611 MS.gene063080:CDS
GGTTACAACGCCGTCCAAGT+TGG 0.618166 3.3:+95910632 MS.gene063080:CDS
AACTCCTGAAGATGGCGCAT+AGG 0.631101 3.3:-95910725 None:intergenic
GTGAAAATGAGCTTAGAGGC+TGG 0.634928 3.3:+95910479 MS.gene063080:CDS
TCTTGCCAACAATCTTAGCG+GGG 0.638407 3.3:-95911206 None:intergenic
CCTCCGGTAACCAACTTGGA+CGG 0.639997 3.3:-95910642 None:intergenic
ATGAGCTTAGAGGCTGGTGT+TGG 0.640133 3.3:+95910485 MS.gene063080:CDS
AAAAGAGGACCAATGAGCCA+TGG 0.640937 3.3:+95910979 MS.gene063080:CDS
CGTCCAAGTTGGTTACCGGA+GGG 0.643249 3.3:+95910643 MS.gene063080:CDS
CTTGCCAACAATCTTAGCGG+GGG 0.643846 3.3:-95911205 None:intergenic
GACGGCGTTGTAACCGTCGG+TGG 0.648118 3.3:-95910624 None:intergenic
TTGGACGGCGTTGTAACCGT+CGG 0.663802 3.3:-95910627 None:intergenic
ACACCTGGTCGCGTATACAA+GGG 0.687415 3.3:+95911045 MS.gene063080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATCTTTCACTAA+TGG + chr3.3:95910911-95910930 MS.gene063080:intron 15.0%
! CTTAATGTTATCATGATCAA+AGG + chr3.3:95911144-95911163 MS.gene063080:CDS 25.0%
! TTTAACAATCTTAAGCTTTC+TGG - chr3.3:95911112-95911131 None:intergenic 25.0%
AAATTGACCAAACCTGAAAT+GGG + chr3.3:95910677-95910696 MS.gene063080:CDS 30.0%
ATGAACTTACTTACTTTGAC+AGG + chr3.3:95910934-95910953 MS.gene063080:intron 30.0%
ATTAGGTTCAAAACCATCAA+CGG - chr3.3:95910762-95910781 None:intergenic 30.0%
TATCAACTCAACAACTAGAA+GGG - chr3.3:95910894-95910913 None:intergenic 30.0%
TGAAAAGCTCATCAAATACA+AGG - chr3.3:95910790-95910809 None:intergenic 30.0%
TTATCAACTCAACAACTAGA+AGG - chr3.3:95910895-95910914 None:intergenic 30.0%
! TTTGATGAGCTTTTCAATGA+AGG + chr3.3:95910794-95910813 MS.gene063080:CDS 30.0%
AAAGCTCATCATTCCCAATT+TGG - chr3.3:95910522-95910541 None:intergenic 35.0%
AACTAGAAGGGAATGCAAAA+AGG - chr3.3:95910882-95910901 None:intergenic 35.0%
AACTTACTTACTTTGACAGG+TGG + chr3.3:95910937-95910956 MS.gene063080:intron 35.0%
AAGCGTCATAACTTCAAAAG+AGG + chr3.3:95910964-95910983 MS.gene063080:CDS 35.0%
ACTAGAAGGGAATGCAAAAA+GGG - chr3.3:95910881-95910900 None:intergenic 35.0%
CAAATTGACCAAACCTGAAA+TGG + chr3.3:95910676-95910695 MS.gene063080:CDS 35.0%
TGAAATGGGTCATCTTGAAA+AGG + chr3.3:95910691-95910710 MS.gene063080:CDS 35.0%
!! ATGAGCTTTTTCCAACCTAA+CGG + chr3.3:95910533-95910552 MS.gene063080:CDS 35.0%
!! TTTCAGGTTTGGTCAATTTG+CGG - chr3.3:95910676-95910695 None:intergenic 35.0%
!! TTTTCAAGATGACCCATTTC+AGG - chr3.3:95910692-95910711 None:intergenic 35.0%
GTAAGCCTGGTAATCTTCTA+CGG + chr3.3:95911178-95911197 MS.gene063080:CDS 40.0%
GTTCTTGCCAACAATCTTAG+CGG - chr3.3:95911211-95911230 None:intergenic 40.0%
TTCTTGCCAACAATCTTAGC+GGG - chr3.3:95911210-95911229 None:intergenic 40.0%
TTTCGTCTTCATTCCGTTGA+TGG + chr3.3:95910746-95910765 MS.gene063080:CDS 40.0%
! TTTGAAACCGACAACAGTGA+CGG - chr3.3:95910570-95910589 None:intergenic 40.0%
!! AAGATGACCCATTTCAGGTT+TGG - chr3.3:95910687-95910706 None:intergenic 40.0%
!! ACTGTTGTCGGTTTCAAAGA+AGG + chr3.3:95910572-95910591 MS.gene063080:CDS 40.0%
!! TCAAATACAAGGCGCTGATT+AGG - chr3.3:95910779-95910798 None:intergenic 40.0%
AAAAGAGGACCAATGAGCCA+TGG + chr3.3:95910979-95910998 MS.gene063080:CDS 45.0%
AAAGAAGATGCCTGGTAGGA+TGG + chr3.3:95911068-95911087 MS.gene063080:CDS 45.0%
AAGAAGATGCCTGGTAGGAT+GGG + chr3.3:95911069-95911088 MS.gene063080:CDS 45.0%
AGAAGATTACCAGGCTTACC+AGG - chr3.3:95911177-95911196 None:intergenic 45.0%
ATGGGTCATCTTGAAAAGGC+CGG + chr3.3:95910695-95910714 MS.gene063080:CDS 45.0%
CACAGACAACTTGGATCCAT+TGG + chr3.3:95911012-95911031 MS.gene063080:CDS 45.0%
GAACAACTTCACCGTTAGGT+TGG - chr3.3:95910547-95910566 None:intergenic 45.0%
GAAGACGAAACTCCTGAAGA+TGG - chr3.3:95910736-95910755 None:intergenic 45.0%
GCTATCCGTAGAAGATTACC+AGG - chr3.3:95911186-95911205 None:intergenic 45.0%
GGTGATCTTGTTGATGTCTC+TGG + chr3.3:95910815-95910834 MS.gene063080:CDS 45.0%
GGTGGTGAAAATGAGCTTAG+AGG + chr3.3:95910475-95910494 MS.gene063080:CDS 45.0%
GTGAAAATGAGCTTAGAGGC+TGG + chr3.3:95910479-95910498 MS.gene063080:CDS 45.0%
TACAAGGGAAAGAAGATGCC+TGG + chr3.3:95911060-95911079 MS.gene063080:CDS 45.0%
TCCAAGAGTCACAGACAACT+TGG + chr3.3:95911003-95911022 MS.gene063080:CDS 45.0%
TCCAAGTTGTCTGTGACTCT+TGG - chr3.3:95911007-95911026 None:intergenic 45.0%
TCTTGCCAACAATCTTAGCG+GGG - chr3.3:95911209-95911228 None:intergenic 45.0%
TTGAAACCGACAACAGTGAC+GGG - chr3.3:95910569-95910588 None:intergenic 45.0%
! AAGCTTTCTGGTCTTCCTGT+TGG - chr3.3:95911100-95911119 None:intergenic 45.0%
!! ATGATCAAAGGTGCTCTTCC+TGG + chr3.3:95911156-95911175 MS.gene063080:CDS 45.0%
!! GGTGTGATGGGTACCAAATT+GGG + chr3.3:95910506-95910525 MS.gene063080:CDS 45.0%
!! TGGTGTGATGGGTACCAAAT+TGG + chr3.3:95910505-95910524 MS.gene063080:CDS 45.0%
AACTCCTGAAGATGGCGCAT+AGG - chr3.3:95910728-95910747 None:intergenic 50.0%
ACACCTGGTCGCGTATACAA+GGG + chr3.3:95911045-95911064 MS.gene063080:CDS 50.0%
ACCTTGAAAACCCTTGCCGA+TGG - chr3.3:95910846-95910865 None:intergenic 50.0%
ACGGGAACAACTTCACCGTT+AGG - chr3.3:95910551-95910570 None:intergenic 50.0%
AGGGAAAGAAGATGCCTGGT+AGG + chr3.3:95911064-95911083 MS.gene063080:CDS 50.0%
ATAGCCCCCGCTAAGATTGT+TGG + chr3.3:95911201-95911220 MS.gene063080:CDS 50.0%
ATGAGCTTAGAGGCTGGTGT+TGG + chr3.3:95910485-95910504 MS.gene063080:CDS 50.0%
ATTTGCGGTCTCTAACCCTC+CGG - chr3.3:95910661-95910680 None:intergenic 50.0%
CAACTTGGATCCATTGGTGC+CGG + chr3.3:95911018-95911037 MS.gene063080:CDS 50.0%
CATTCCTATGCGCCATCTTC+AGG + chr3.3:95910721-95910740 MS.gene063080:CDS 50.0%
CTCTTGGAACCATGGCTCAT+TGG - chr3.3:95910991-95911010 None:intergenic 50.0%
CTGAAGATGGCGCATAGGAA+TGG - chr3.3:95910723-95910742 None:intergenic 50.0%
CTTGCCAACAATCTTAGCGG+GGG - chr3.3:95911208-95911227 None:intergenic 50.0%
GTCTGTGACTCTTGGAACCA+TGG - chr3.3:95910999-95911018 None:intergenic 50.0%
GTTGTTCCCGTCACTGTTGT+CGG + chr3.3:95910560-95910579 MS.gene063080:CDS 50.0%
TAACCCTCCGGTAACCAACT+TGG - chr3.3:95910649-95910668 None:intergenic 50.0%
TACACCTGGTCGCGTATACA+AGG + chr3.3:95911044-95911063 MS.gene063080:CDS 50.0%
TTGAAAACCCTTGCCGATGG+TGG - chr3.3:95910843-95910862 None:intergenic 50.0%
TTTCCCTTGTATACGCGACC+AGG - chr3.3:95911051-95911070 None:intergenic 50.0%
! ACCATCGGCAAGGGTTTTCA+AGG + chr3.3:95910842-95910861 MS.gene063080:CDS 50.0%
AAGATGCCTGGTAGGATGGG+TGG + chr3.3:95911072-95911091 MS.gene063080:CDS 55.0%
AGGTGTAGTTCCGGCACCAA+TGG - chr3.3:95911031-95911050 None:intergenic 55.0%
ATCAAAACCGACGCCACCGA+CGG + chr3.3:95910611-95910630 MS.gene063080:CDS 55.0%
ATGGCGCATAGGAATGGCTC+CGG - chr3.3:95910717-95910736 None:intergenic 55.0%
CCTCCGGTAACCAACTTGGA+CGG - chr3.3:95910645-95910664 None:intergenic 55.0%
GATGTCTCTGGCACCACCAT+CGG + chr3.3:95910827-95910846 MS.gene063080:CDS 55.0%
GGTTACAACGCCGTCCAAGT+TGG + chr3.3:95910632-95910651 MS.gene063080:CDS 55.0%
GTAGGATGGGTGGAACCAAC+AGG + chr3.3:95911082-95911101 MS.gene063080:CDS 55.0%
TACGCGACCAGGTGTAGTTC+CGG - chr3.3:95911040-95911059 None:intergenic 55.0%
TTGGACGGCGTTGTAACCGT+CGG - chr3.3:95910630-95910649 None:intergenic 55.0%
TTGGTTCCACCCATCCTACC+AGG - chr3.3:95911081-95911100 None:intergenic 55.0%
!! ACGCCGTCCAAGTTGGTTAC+CGG + chr3.3:95910639-95910658 MS.gene063080:CDS 55.0%
!! AGAGGCTGGTGTTGGTGTGA+TGG + chr3.3:95910493-95910512 MS.gene063080:CDS 55.0%
!! ATTGGTGCCGGAACTACACC+TGG + chr3.3:95911030-95911049 MS.gene063080:CDS 55.0%
!! CGTCCAAGTTGGTTACCGGA+GGG + chr3.3:95910643-95910662 MS.gene063080:CDS 55.0%
!! GAGGCTGGTGTTGGTGTGAT+GGG + chr3.3:95910494-95910513 MS.gene063080:CDS 55.0%
GGTGCTCTTCCTGGTAAGCC+TGG + chr3.3:95911165-95911184 MS.gene063080:CDS 60.0%
GTTGTAACCGTCGGTGGCGT+CGG - chr3.3:95910621-95910640 None:intergenic 60.0%
TCTGGCACCACCATCGGCAA+GGG + chr3.3:95910833-95910852 MS.gene063080:CDS 60.0%
!! CCGTCCAAGTTGGTTACCGG+AGG + chr3.3:95910642-95910661 MS.gene063080:CDS 60.0%
CTCTGGCACCACCATCGGCA+AGG + chr3.3:95910832-95910851 MS.gene063080:CDS 65.0%
GACGGCGTTGTAACCGTCGG+TGG - chr3.3:95910627-95910646 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 95910473 95911245 95910473 ID=MS.gene063080
chr3.3 mRNA 95910473 95911245 95910473 ID=MS.gene063080.t1;Parent=MS.gene063080
chr3.3 exon 95910473 95910863 95910473 ID=MS.gene063080.t1.exon1;Parent=MS.gene063080.t1
chr3.3 CDS 95910473 95910863 95910473 ID=cds.MS.gene063080.t1;Parent=MS.gene063080.t1
chr3.3 exon 95910956 95911245 95910956 ID=MS.gene063080.t1.exon2;Parent=MS.gene063080.t1
chr3.3 CDS 95910956 95911245 95910956 ID=cds.MS.gene063080.t1;Parent=MS.gene063080.t1
Gene Sequence

>MS.gene063080

ATGGTGGTGAAAATGAGCTTAGAGGCTGGTGTTGGTGTGATGGGTACCAAATTGGGAATGATGAGCTTTTTCCAACCTAACGGTGAAGTTGTTCCCGTCACTGTTGTCGGTTTCAAAGAAGGCAACATCGTCACTCAAATCAAAACCGACGCCACCGACGGTTACAACGCCGTCCAAGTTGGTTACCGGAGGGTTAGAGACCGCAAATTGACCAAACCTGAAATGGGTCATCTTGAAAAGGCCGGAGCCATTCCTATGCGCCATCTTCAGGAGTTTCGTCTTCATTCCGTTGATGGTTTTGAACCTAATCAGCGCCTTGTATTTGATGAGCTTTTCAATGAAGGTGATCTTGTTGATGTCTCTGGCACCACCATCGGCAAGGGTTTTCAAGGTCTGTAACTCTCTCCCTTTTTGCATTCCCTTCTAGTTGTTGAGTTGATAATAATATCTTTCACTAATGGATGAACTTACTTACTTTGACAGGTGGTATCAAGCGTCATAACTTCAAAAGAGGACCAATGAGCCATGGTTCCAAGAGTCACAGACAACTTGGATCCATTGGTGCCGGAACTACACCTGGTCGCGTATACAAGGGAAAGAAGATGCCTGGTAGGATGGGTGGAACCAACAGGAAGACCAGAAAGCTTAAGATTGTTAAAATTGATAAAGATCTTAATGTTATCATGATCAAAGGTGCTCTTCCTGGTAAGCCTGGTAATCTTCTACGGATAGCCCCCGCTAAGATTGTTGGCAAGAACATTCCAAAGAATTAA

Protein sequence

>MS.gene063080.t1

MVVKMSLEAGVGVMGTKLGMMSFFQPNGEVVPVTVVGFKEGNIVTQIKTDATDGYNAVQVGYRRVRDRKLTKPEMGHLEKAGAIPMRHLQEFRLHSVDGFEPNQRLVFDELFNEGDLVDVSGTTIGKGFQGGIKRHNFKRGPMSHGSKSHRQLGSIGAGTTPGRVYKGKKMPGRMGGTNRKTRKLKIVKIDKDLNVIMIKGALPGKPGNLLRIAPAKIVGKNIPKN