Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061166.t1 | XP_003592267.1 | 100 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 9.20E-52 | 212.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061166.t1 | Q9LZ57 | 83.0 | 106 | 17 | 1 | 7 | 111 | 3 | 108 | 3.1e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061166.t1 | G7IAL7 | 100.0 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 6.6e-52 | 212.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061166 | MS.gene59609 | PPI |
| MS.gene061166 | MS.gene87309 | PPI |
| MS.gene061166 | MS.gene34149 | PPI |
| MS.gene061166 | MS.gene79721 | PPI |
| MS.gene061166 | MS.gene98171 | PPI |
| MS.gene061166 | MS.gene92142 | PPI |
| MS.gene061166 | MS.gene49109 | PPI |
| MS.gene061166 | MS.gene95828 | PPI |
| MS.gene061166 | MS.gene006276 | PPI |
| MS.gene061166 | MS.gene83961 | PPI |
| MS.gene061166 | MS.gene81493 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061166.t1 | MTR_1g100960 | 100.000 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 7.15e-75 | 217 |
| MS.gene061166.t1 | MTR_6g053730 | 96.396 | 111 | 4 | 0 | 1 | 111 | 1 | 111 | 3.38e-72 | 210 |
| MS.gene061166.t1 | MTR_1g100960 | 100.000 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 6.71e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061166.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene061166.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene061166.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene061166.t1 | AT2G37600 | 82.692 | 104 | 18 | 0 | 8 | 111 | 8 | 111 | 2.05e-56 | 171 |
| MS.gene061166.t1 | AT2G37600 | 82.692 | 104 | 18 | 0 | 8 | 111 | 8 | 111 | 2.05e-56 | 171 |
| MS.gene061166.t1 | AT5G02450 | 83.019 | 106 | 17 | 1 | 7 | 111 | 3 | 108 | 8.05e-56 | 169 |
| MS.gene061166.t1 | AT3G53740 | 72.072 | 111 | 22 | 1 | 1 | 111 | 1 | 102 | 3.04e-49 | 152 |
Find 36 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAGTACTTGGTTGTTT+AGG | 0.306770 | 1.4:-77964204 | None:intergenic |
| CTTCTTCTTTGCGCGCTTGT+GGG | 0.315293 | 1.4:-77965001 | None:intergenic |
| ACGTGGAGCCAATTCCTTCT+TGG | 0.386816 | 1.4:-77964259 | None:intergenic |
| AAAAGACAAGAGGGCACTTA+AGG | 0.398330 | 1.4:+77964957 | MS.gene061166:CDS |
| TCTTCTTCTTTGCGCGCTTG+TGG | 0.421394 | 1.4:-77965002 | None:intergenic |
| TCCACGTCCTTCAGCTCGCA+AGG | 0.427898 | 1.4:+77964275 | MS.gene061166:CDS |
| TGCAGGGCTGGTGGTGGAGC+AGG | 0.430321 | 1.4:+77965173 | MS.gene061166:intron |
| AGCAGGAGATAAGAAGAAAT+AGG | 0.434078 | 1.4:+77965190 | MS.gene061166:CDS |
| CATCGTCACCAAGAAGGAAT+TGG | 0.434230 | 1.4:+77964251 | MS.gene061166:CDS |
| CCAAGTACTGGTTTGTTCGT+TGG | 0.458873 | 1.4:+77964213 | MS.gene061166:CDS |
| AAGGTTGCAAAGAGAAAGCT+TGG | 0.461674 | 1.4:+77964976 | MS.gene061166:CDS |
| AACCTCATCAGAGAGGTTGC+TGG | 0.461744 | 1.4:+77964889 | MS.gene061166:CDS |
| CCAACGAACAAACCAGTACT+TGG | 0.464574 | 1.4:-77964213 | None:intergenic |
| TCAGTTATACGCTTCTCATA+AGG | 0.487217 | 1.4:-77964919 | None:intergenic |
| TCAATGTGCAGGGCTGGTGG+TGG | 0.508415 | 1.4:+77965167 | MS.gene061166:intron |
| AGGAGATGTCTAATGTCCTC+AGG | 0.510350 | 1.4:+77965028 | MS.gene061166:CDS |
| CCCTTGCGAGCTGAAGGACG+TGG | 0.511446 | 1.4:-77964276 | None:intergenic |
| ATAACTGAGTTGCTCAAGGT+TGG | 0.521401 | 1.4:+77964934 | MS.gene061166:CDS |
| CCACGTCCTTCAGCTCGCAA+GGG | 0.521414 | 1.4:+77964276 | MS.gene061166:CDS |
| GCTCCTAAACAACCAAGTAC+TGG | 0.551611 | 1.4:+77964201 | MS.gene061166:CDS |
| CACGTCCTTCAGCTCGCAAG+GGG | 0.561496 | 1.4:+77964277 | MS.gene061166:CDS |
| TTGTTCGTTGGATTGAACAA+AGG | 0.583526 | 1.4:+77964225 | MS.gene061166:CDS |
| AGGCCACATCGTCACCAAGA+AGG | 0.591656 | 1.4:+77964245 | MS.gene061166:CDS |
| ATTCCTTCTTGGTGACGATG+TGG | 0.592033 | 1.4:-77964248 | None:intergenic |
| ATCCAGCAACCTCTCTGATG+AGG | 0.615697 | 1.4:-77964891 | None:intergenic |
| TGCTTCAGAAAACAAGCAAG+AGG | 0.621594 | 1.4:+77964851 | MS.gene061166:intron |
| GCAAGAGGGTGCACTTTGTG+AGG | 0.622900 | 1.4:+77964866 | MS.gene061166:CDS |
| GCGTATAACTGAGTTGCTCA+AGG | 0.623591 | 1.4:+77964930 | MS.gene061166:CDS |
| GCGCGCAAAGAAGAAGAGAG+AGG | 0.624842 | 1.4:+77965008 | MS.gene061166:CDS |
| CTAATGTCCTCAGGAAGATG+AGG | 0.627117 | 1.4:+77965037 | MS.gene061166:CDS |
| CAAGGTTGGAAAAGACAAGA+GGG | 0.628294 | 1.4:+77964948 | MS.gene061166:CDS |
| TCAAGGTTGGAAAAGACAAG+AGG | 0.663036 | 1.4:+77964947 | MS.gene061166:CDS |
| GCTTCAGAAAACAAGCAAGA+GGG | 0.668902 | 1.4:+77964852 | MS.gene061166:intron |
| GAACAAACCTCATCTTCCTG+AGG | 0.694769 | 1.4:-77965044 | None:intergenic |
| ACGTCCTTCAGCTCGCAAGG+GGG | 0.706112 | 1.4:+77964278 | MS.gene061166:CDS |
| TGTGAGGAACCTCATCAGAG+AGG | 0.717293 | 1.4:+77964882 | MS.gene061166:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCAGAAAATTATAAATT+AGG | - | chr1.4:77964395-77964414 | None:intergenic | 10.0% |
| !!! | TTTATAATTTTCTGAATTTT+TGG | + | chr1.4:77964397-77964416 | MS.gene061166:intron | 10.0% |
| !! | GAATTTATGAATTAACATAA+AGG | + | chr1.4:77964620-77964639 | MS.gene061166:intron | 15.0% |
| !! | AAGCAATTAAAACATTCTTT+AGG | - | chr1.4:77964836-77964855 | None:intergenic | 20.0% |
| !! | AGCAATTAAAACATTCTTTA+GGG | - | chr1.4:77964835-77964854 | None:intergenic | 20.0% |
| !! | TGATTCAAAACTATAATAAC+AGG | - | chr1.4:77964333-77964352 | None:intergenic | 20.0% |
| !! | TTTATTTATTCTGTTCATCT+TGG | + | chr1.4:77964803-77964822 | MS.gene061166:intron | 20.0% |
| !! | TTTGTTAATATTGTTATGCT+TGG | + | chr1.4:77965075-77965094 | MS.gene061166:intron | 20.0% |
| !!! | GTTTTTTATGATATGCATTT+CGG | + | chr1.4:77965104-77965123 | MS.gene061166:intron | 20.0% |
| !!! | TAAGCTCTTAATTTGTATTA+AGG | + | chr1.4:77964481-77964500 | MS.gene061166:intron | 20.0% |
| !!! | TAGATTTAACTTTGAATTAC+TGG | + | chr1.4:77964437-77964456 | MS.gene061166:intron | 20.0% |
| ! | AACAACATTTCATCCTATTA+AGG | - | chr1.4:77964785-77964804 | None:intergenic | 25.0% |
| !! | TATATCTCATAGACTGATTT+AGG | - | chr1.4:77964712-77964731 | None:intergenic | 25.0% |
| !! | TTAGATATTTTCAATGTGCA+GGG | + | chr1.4:77965157-77965176 | MS.gene061166:intron | 25.0% |
| !! | TTTAGATATTTTCAATGTGC+AGG | + | chr1.4:77965156-77965175 | MS.gene061166:intron | 25.0% |
| !!! | AACTTTGAATTACTGGTTTA+AGG | + | chr1.4:77964444-77964463 | MS.gene061166:intron | 25.0% |
| AACATTTCATCCTATTAAGG+TGG | - | chr1.4:77964782-77964801 | None:intergenic | 30.0% | |
| CTATGAGATATATGACTAGA+AGG | + | chr1.4:77964720-77964739 | MS.gene061166:intron | 30.0% | |
| TTTACACTATCCACCTTAAT+AGG | + | chr1.4:77964769-77964788 | MS.gene061166:intron | 30.0% | |
| AAACCAGTACTTGGTTGTTT+AGG | - | chr1.4:77964207-77964226 | None:intergenic | 35.0% | |
| ATTTGTATTAAGGCTGCGTT+TGG | + | chr1.4:77964491-77964510 | MS.gene061166:intron | 35.0% | |
| TATTCTGTTCATCTTGGATG+TGG | + | chr1.4:77964809-77964828 | MS.gene061166:intron | 35.0% | |
| TCAGTTATACGCTTCTCATA+AGG | - | chr1.4:77964922-77964941 | None:intergenic | 35.0% | |
| TTGTTCGTTGGATTGAACAA+AGG | + | chr1.4:77964225-77964244 | MS.gene061166:CDS | 35.0% | |
| ! | AGACAAGTACTGTATTGGAA+TGG | - | chr1.4:77964361-77964380 | None:intergenic | 35.0% |
| ! | AGACTGATTTAGGACTTGTT+TGG | - | chr1.4:77964702-77964721 | None:intergenic | 35.0% |
| ! | TAGAGAGACAAGTACTGTAT+TGG | - | chr1.4:77964366-77964385 | None:intergenic | 35.0% |
| AAGGTTGCAAAGAGAAAGCT+TGG | + | chr1.4:77964976-77964995 | MS.gene061166:CDS | 40.0% | |
| AGGAGAAACGTGTGATGTAT+TGG | + | chr1.4:77964740-77964759 | MS.gene061166:intron | 40.0% | |
| ATAACTGAGTTGCTCAAGGT+TGG | + | chr1.4:77964934-77964953 | MS.gene061166:CDS | 40.0% | |
| CAAGGTTGGAAAAGACAAGA+GGG | + | chr1.4:77964948-77964967 | MS.gene061166:CDS | 40.0% | |
| GCTTCAGAAAACAAGCAAGA+GGG | + | chr1.4:77964852-77964871 | MS.gene061166:intron | 40.0% | |
| GGAGAAACGTGTGATGTATT+GGG | + | chr1.4:77964741-77964760 | MS.gene061166:intron | 40.0% | |
| TATTAAGGCTGCGTTTGGAT+TGG | + | chr1.4:77964496-77964515 | MS.gene061166:intron | 40.0% | |
| TCAAGGTTGGAAAAGACAAG+AGG | + | chr1.4:77964947-77964966 | MS.gene061166:CDS | 40.0% | |
| TGCTTCAGAAAACAAGCAAG+AGG | + | chr1.4:77964851-77964870 | MS.gene061166:intron | 40.0% | |
| ! | ATATTTTCAATGTGCAGGGC+TGG | + | chr1.4:77965161-77965180 | MS.gene061166:intron | 40.0% |
| ! | TCGGAATGCGTTCTTGTTAT+TGG | + | chr1.4:77965123-77965142 | MS.gene061166:intron | 40.0% |
| !! | AAAAGACAAGAGGGCACTTA+AGG | + | chr1.4:77964957-77964976 | MS.gene061166:CDS | 40.0% |
| AGGAGATGTCTAATGTCCTC+AGG | + | chr1.4:77965028-77965047 | MS.gene061166:CDS | 45.0% | |
| CATCGTCACCAAGAAGGAAT+TGG | + | chr1.4:77964251-77964270 | MS.gene061166:CDS | 45.0% | |
| CCAACGAACAAACCAGTACT+TGG | - | chr1.4:77964216-77964235 | None:intergenic | 45.0% | |
| CTAATGTCCTCAGGAAGATG+AGG | + | chr1.4:77965037-77965056 | MS.gene061166:CDS | 45.0% | |
| GAACAAACCTCATCTTCCTG+AGG | - | chr1.4:77965047-77965066 | None:intergenic | 45.0% | |
| GCGTATAACTGAGTTGCTCA+AGG | + | chr1.4:77964930-77964949 | MS.gene061166:CDS | 45.0% | |
| GCTCCTAAACAACCAAGTAC+TGG | + | chr1.4:77964201-77964220 | MS.gene061166:CDS | 45.0% | |
| !! | ATTCCTTCTTGGTGACGATG+TGG | - | chr1.4:77964251-77964270 | None:intergenic | 45.0% |
| !! | CCAAGTACTGGTTTGTTCGT+TGG | + | chr1.4:77964213-77964232 | MS.gene061166:CDS | 45.0% |
| AACCTCATCAGAGAGGTTGC+TGG | + | chr1.4:77964889-77964908 | MS.gene061166:CDS | 50.0% | |
| ACGTGGAGCCAATTCCTTCT+TGG | - | chr1.4:77964262-77964281 | None:intergenic | 50.0% | |
| ATCCAGCAACCTCTCTGATG+AGG | - | chr1.4:77964894-77964913 | None:intergenic | 50.0% | |
| CTTCTTCTTTGCGCGCTTGT+GGG | - | chr1.4:77965004-77965023 | None:intergenic | 50.0% | |
| TCTTCTTCTTTGCGCGCTTG+TGG | - | chr1.4:77965005-77965024 | None:intergenic | 50.0% | |
| TGTGAGGAACCTCATCAGAG+AGG | + | chr1.4:77964882-77964901 | MS.gene061166:CDS | 50.0% | |
| ! | TTTTCAATGTGCAGGGCTGG+TGG | + | chr1.4:77965164-77965183 | MS.gene061166:intron | 50.0% |
| AGGCCACATCGTCACCAAGA+AGG | + | chr1.4:77964245-77964264 | MS.gene061166:CDS | 55.0% | |
| GCGCGCAAAGAAGAAGAGAG+AGG | + | chr1.4:77965008-77965027 | MS.gene061166:CDS | 55.0% | |
| ! | GCAAGAGGGTGCACTTTGTG+AGG | + | chr1.4:77964866-77964885 | MS.gene061166:CDS | 55.0% |
| ACGTCCTTCAGCTCGCAAGG+GGG | + | chr1.4:77964278-77964297 | MS.gene061166:CDS | 60.0% | |
| CACGTCCTTCAGCTCGCAAG+GGG | + | chr1.4:77964277-77964296 | MS.gene061166:CDS | 60.0% | |
| CCACGTCCTTCAGCTCGCAA+GGG | + | chr1.4:77964276-77964295 | MS.gene061166:CDS | 60.0% | |
| TCCACGTCCTTCAGCTCGCA+AGG | + | chr1.4:77964275-77964294 | MS.gene061166:CDS | 60.0% | |
| ! | TCAATGTGCAGGGCTGGTGG+TGG | + | chr1.4:77965167-77965186 | MS.gene061166:intron | 60.0% |
| CCCTTGCGAGCTGAAGGACG+TGG | - | chr1.4:77964279-77964298 | None:intergenic | 65.0% | |
| CTGACCCCCTTGCGAGCTGA+AGG | - | chr1.4:77964285-77964304 | None:intergenic | 65.0% | |
| !! | TGCAGGGCTGGTGGTGGAGC+AGG | + | chr1.4:77965173-77965192 | MS.gene061166:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 77964198 | 77965211 | 77964198 | ID=MS.gene061166 |
| chr1.4 | mRNA | 77964198 | 77965211 | 77964198 | ID=MS.gene061166.t1;Parent=MS.gene061166 |
| chr1.4 | exon | 77964198 | 77964299 | 77964198 | ID=MS.gene061166.t1.exon1;Parent=MS.gene061166.t1 |
| chr1.4 | CDS | 77964198 | 77964299 | 77964198 | ID=cds.MS.gene061166.t1;Parent=MS.gene061166.t1 |
| chr1.4 | exon | 77964859 | 77965058 | 77964859 | ID=MS.gene061166.t1.exon2;Parent=MS.gene061166.t1 |
| chr1.4 | CDS | 77964859 | 77965058 | 77964859 | ID=cds.MS.gene061166.t1;Parent=MS.gene061166.t1 |
| chr1.4 | exon | 77965178 | 77965211 | 77965178 | ID=MS.gene061166.t1.exon3;Parent=MS.gene061166.t1 |
| chr1.4 | CDS | 77965178 | 77965211 | 77965178 | ID=cds.MS.gene061166.t1;Parent=MS.gene061166.t1 |
| Gene Sequence |
| Protein sequence |
