Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34149.t1 | XP_003592267.1 | 100 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 9.20E-52 | 212.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34149.t1 | Q9LZ57 | 83.0 | 106 | 17 | 1 | 7 | 111 | 3 | 108 | 3.1e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34149.t1 | G7IAL7 | 100.0 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 6.6e-52 | 212.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene34149 | 0.862749 | 4.06E-64 | -1.69E-46 |
| MS.gene051746 | MS.gene34149 | 0.854737 | 1.01E-61 | -1.69E-46 |
| MS.gene051748 | MS.gene34149 | 0.840095 | 1.07E-57 | -1.69E-46 |
| MS.gene051903 | MS.gene34149 | 0.855249 | 7.16E-62 | -1.69E-46 |
| MS.gene052947 | MS.gene34149 | 0.807045 | 6.12E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene34149 | 0.876909 | 9.59E-69 | -1.69E-46 |
| MS.gene056075 | MS.gene34149 | 0.811642 | 6.32E-51 | -1.69E-46 |
| MS.gene057094 | MS.gene34149 | 0.818032 | 2.42E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene34149 | 0.814898 | 1.22E-51 | -1.69E-46 |
| MS.gene060856 | MS.gene34149 | 0.845737 | 3.37E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061166 | MS.gene34149 | PPI |
| MS.gene79721 | MS.gene34149 | PPI |
| MS.gene83961 | MS.gene34149 | PPI |
| MS.gene81493 | MS.gene34149 | PPI |
| MS.gene59609 | MS.gene34149 | PPI |
| MS.gene006276 | MS.gene34149 | PPI |
| MS.gene92142 | MS.gene34149 | PPI |
| MS.gene49109 | MS.gene34149 | PPI |
| MS.gene95828 | MS.gene34149 | PPI |
| MS.gene34149 | MS.gene98171 | PPI |
| MS.gene87309 | MS.gene34149 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34149.t1 | MTR_1g100960 | 100.000 | 111 | 0 | 0 | 1 | 111 | 1 | 111 | 7.15e-75 | 217 |
| MS.gene34149.t1 | MTR_6g053730 | 96.396 | 111 | 4 | 0 | 1 | 111 | 1 | 111 | 3.38e-72 | 210 |
| MS.gene34149.t1 | MTR_1g100960 | 100.000 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 6.71e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34149.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene34149.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene34149.t1 | AT3G53740 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.86e-58 | 176 |
| MS.gene34149.t1 | AT2G37600 | 82.692 | 104 | 18 | 0 | 8 | 111 | 8 | 111 | 2.05e-56 | 171 |
| MS.gene34149.t1 | AT2G37600 | 82.692 | 104 | 18 | 0 | 8 | 111 | 8 | 111 | 2.05e-56 | 171 |
| MS.gene34149.t1 | AT5G02450 | 83.019 | 106 | 17 | 1 | 7 | 111 | 3 | 108 | 8.05e-56 | 169 |
| MS.gene34149.t1 | AT3G53740 | 72.072 | 111 | 22 | 1 | 1 | 111 | 1 | 102 | 3.04e-49 | 152 |
Find 36 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAGTACTTGGTTGTTT+AGG | 0.306770 | 1.2:-75191705 | None:intergenic |
| CTTCTTCTTTGCACGTTTGT+GGG | 0.313110 | 1.2:-75192375 | None:intergenic |
| TCTTCTTCTTTGCACGTTTG+TGG | 0.365751 | 1.2:-75192376 | None:intergenic |
| AAAAGACAAGAGGGCACTTA+AGG | 0.398330 | 1.2:+75192331 | MS.gene34149:CDS |
| TCCACGTCCTTCAGCTCGCA+AGG | 0.427898 | 1.2:+75191776 | MS.gene34149:CDS |
| TGCAGGGCTGGTGGTGGAGC+AGG | 0.430321 | 1.2:+75192546 | MS.gene34149:intron |
| AGCAGGAGATAAGAAGAAAT+AGG | 0.434078 | 1.2:+75192563 | MS.gene34149:CDS |
| CATCGTCACAAAGAAGGAAT+TGG | 0.434230 | 1.2:+75191752 | MS.gene34149:CDS |
| CCAAGTACTGGTTTGTTCGT+TGG | 0.458873 | 1.2:+75191714 | MS.gene34149:CDS |
| AAGGTTGCAAAGAGAAAGCT+TGG | 0.461674 | 1.2:+75192350 | MS.gene34149:CDS |
| CCAACGAACAAACCAGTACT+TGG | 0.464574 | 1.2:-75191714 | None:intergenic |
| AACCTCATTAGAGAGGTTGC+TGG | 0.487721 | 1.2:+75192263 | MS.gene34149:CDS |
| ATCACTGAGTTGCTCAAGGT+TGG | 0.503868 | 1.2:+75192308 | MS.gene34149:CDS |
| TCAATGTGCAGGGCTGGTGG+TGG | 0.508415 | 1.2:+75192540 | MS.gene34149:intron |
| AGGAGATGTCTAATGTCCTC+AGG | 0.510350 | 1.2:+75192402 | MS.gene34149:CDS |
| ACCTCATTAGAGAGGTTGCT+GGG | 0.511316 | 1.2:+75192264 | MS.gene34149:CDS |
| CCCTTGCGAGCTGAAGGACG+TGG | 0.511446 | 1.2:-75191777 | None:intergenic |
| CCACGTCCTTCAGCTCGCAA+GGG | 0.521414 | 1.2:+75191777 | MS.gene34149:CDS |
| AGGCCACATCGTCACAAAGA+AGG | 0.551215 | 1.2:+75191746 | MS.gene34149:CDS |
| GCTCCTAAACAACCAAGTAC+TGG | 0.551611 | 1.2:+75191702 | MS.gene34149:CDS |
| CACGTCCTTCAGCTCGCAAG+GGG | 0.561496 | 1.2:+75191778 | MS.gene34149:CDS |
| ATTCCTTCTTTGTGACGATG+TGG | 0.577252 | 1.2:-75191749 | None:intergenic |
| TTGTTCGTTGGATTGAACAA+AGG | 0.583526 | 1.2:+75191726 | MS.gene34149:CDS |
| TCAGTGATACGCTTCTCGTA+AGG | 0.584616 | 1.2:-75192293 | None:intergenic |
| ACCCAGCAACCTCTCTAATG+AGG | 0.601885 | 1.2:-75192265 | None:intergenic |
| TGTGAGGAACCTCATTAGAG+AGG | 0.602916 | 1.2:+75192256 | MS.gene34149:CDS |
| TCGTTCAGAAAACAAGCAAG+AGG | 0.606732 | 1.2:+75192225 | MS.gene34149:intron |
| ACGTGCAAAGAAGAAGAGAG+AGG | 0.618868 | 1.2:+75192382 | MS.gene34149:CDS |
| GCAAGAGGGTGCACTTTGTG+AGG | 0.622900 | 1.2:+75192240 | MS.gene34149:CDS |
| CTAATGTCCTCAGGAAGATG+AGG | 0.627117 | 1.2:+75192411 | MS.gene34149:CDS |
| CAAGGTTGGAAAAGACAAGA+GGG | 0.628294 | 1.2:+75192322 | MS.gene34149:CDS |
| GCGTATCACTGAGTTGCTCA+AGG | 0.629950 | 1.2:+75192304 | MS.gene34149:CDS |
| CGTTCAGAAAACAAGCAAGA+GGG | 0.632672 | 1.2:+75192226 | MS.gene34149:intron |
| TCAAGGTTGGAAAAGACAAG+AGG | 0.663036 | 1.2:+75192321 | MS.gene34149:CDS |
| GAACAAACCTCATCTTCCTG+AGG | 0.694769 | 1.2:-75192418 | None:intergenic |
| ACGTCCTTCAGCTCGCAAGG+GGG | 0.706112 | 1.2:+75191779 | MS.gene34149:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAAATCTAACATAAAA+TGG | - | chr1.2:75191851-75191870 | None:intergenic | 10.0% |
| !!! | ATTTTATGTTAGATTTAAAT+TGG | + | chr1.2:75191850-75191869 | MS.gene34149:intron | 10.0% |
| !! | AACGAATAAAAACAATCTTT+AGG | - | chr1.2:75192210-75192229 | None:intergenic | 20.0% |
| !! | ACGAATAAAAACAATCTTTA+GGG | - | chr1.2:75192209-75192228 | None:intergenic | 20.0% |
| !!! | AAATTGGAATTATTAGCTTA+AGG | + | chr1.2:75191866-75191885 | MS.gene34149:intron | 20.0% |
| !!! | TTTGTTATTATTGTTATGCT+TGG | + | chr1.2:75192448-75192467 | MS.gene34149:intron | 20.0% |
| ! | AATAGAAGGAGAAATAATGT+TGG | + | chr1.2:75192091-75192110 | MS.gene34149:intron | 25.0% |
| ! | ATAGAAGGAGAAATAATGTT+GGG | + | chr1.2:75192092-75192111 | MS.gene34149:intron | 25.0% |
| ! | ATTAATTCATAAGCTTGTCA+AGG | + | chr1.2:75191999-75192018 | MS.gene34149:intron | 25.0% |
| ! | CATTATTTCATTCATTCCTA+TGG | - | chr1.2:75192140-75192159 | None:intergenic | 25.0% |
| ! | CGAATAAAAACAATCTTTAG+GGG | - | chr1.2:75192208-75192227 | None:intergenic | 25.0% |
| ! | GTTATTATGATAAACATTCC+CGG | + | chr1.2:75192477-75192496 | MS.gene34149:intron | 25.0% |
| ! | TTAAGAAAAAACTGCTTATG+AGG | + | chr1.2:75192026-75192045 | MS.gene34149:intron | 25.0% |
| ! | TTATGAGGATGATATTTATG+AGG | + | chr1.2:75192041-75192060 | MS.gene34149:intron | 25.0% |
| ! | TTATTGTTATGCTTGGTATT+TGG | + | chr1.2:75192455-75192474 | MS.gene34149:intron | 25.0% |
| !! | TTAGATATTTTCAATGTGCA+GGG | + | chr1.2:75192530-75192549 | MS.gene34149:intron | 25.0% |
| !! | TTTAGATATTTTCAATGTGC+AGG | + | chr1.2:75192529-75192548 | MS.gene34149:intron | 25.0% |
| CTTTGTATTAAGGATCTGTT+TGG | + | chr1.2:75191925-75191944 | MS.gene34149:intron | 30.0% | |
| TAGGGAGATATATGAATAGA+AGG | + | chr1.2:75192077-75192096 | MS.gene34149:intron | 30.0% | |
| TATGCTCGTACTTTGTATTA+AGG | + | chr1.2:75191915-75191934 | MS.gene34149:intron | 30.0% | |
| TATTAAGGATCTGTTTGGAT+TGG | + | chr1.2:75191930-75191949 | MS.gene34149:intron | 30.0% | |
| TATTTCATTCATTCCTATGG+TGG | - | chr1.2:75192137-75192156 | None:intergenic | 30.0% | |
| ! | ATGAGGATGACATTTTTGTT+AGG | + | chr1.2:75192058-75192077 | MS.gene34149:intron | 30.0% |
| ! | TGAGGATGACATTTTTGTTA+GGG | + | chr1.2:75192059-75192078 | MS.gene34149:intron | 30.0% |
| ! | TTTTTGCATTCTGTTCATCT+TGG | + | chr1.2:75192176-75192195 | MS.gene34149:intron | 30.0% |
| AAACCAGTACTTGGTTGTTT+AGG | - | chr1.2:75191708-75191727 | None:intergenic | 35.0% | |
| ATACACTATCTATCCACCAT+AGG | + | chr1.2:75192121-75192140 | MS.gene34149:intron | 35.0% | |
| CAATAACAAGAACATGTTCC+GGG | - | chr1.2:75192498-75192517 | None:intergenic | 35.0% | |
| CCAATAACAAGAACATGTTC+CGG | - | chr1.2:75192499-75192518 | None:intergenic | 35.0% | |
| TTGTTCGTTGGATTGAACAA+AGG | + | chr1.2:75191726-75191745 | MS.gene34149:CDS | 35.0% | |
| AAGGTTGCAAAGAGAAAGCT+TGG | + | chr1.2:75192350-75192369 | MS.gene34149:CDS | 40.0% | |
| ATTCCTTCTTTGTGACGATG+TGG | - | chr1.2:75191752-75191771 | None:intergenic | 40.0% | |
| CAAGGTTGGAAAAGACAAGA+GGG | + | chr1.2:75192322-75192341 | MS.gene34149:CDS | 40.0% | |
| CATCGTCACAAAGAAGGAAT+TGG | + | chr1.2:75191752-75191771 | MS.gene34149:CDS | 40.0% | |
| CATTCTGTTCATCTTGGATG+TGG | + | chr1.2:75192182-75192201 | MS.gene34149:intron | 40.0% | |
| CGTTCAGAAAACAAGCAAGA+GGG | + | chr1.2:75192226-75192245 | MS.gene34149:intron | 40.0% | |
| CTTCTTCTTTGCACGTTTGT+GGG | - | chr1.2:75192378-75192397 | None:intergenic | 40.0% | |
| TCAAGGTTGGAAAAGACAAG+AGG | + | chr1.2:75192321-75192340 | MS.gene34149:CDS | 40.0% | |
| TCGTTCAGAAAACAAGCAAG+AGG | + | chr1.2:75192225-75192244 | MS.gene34149:intron | 40.0% | |
| TCTTCTTCTTTGCACGTTTG+TGG | - | chr1.2:75192379-75192398 | None:intergenic | 40.0% | |
| ! | ATATTTTCAATGTGCAGGGC+TGG | + | chr1.2:75192534-75192553 | MS.gene34149:intron | 40.0% |
| ! | CCGGAACATGTTCTTGTTAT+TGG | + | chr1.2:75192496-75192515 | MS.gene34149:intron | 40.0% |
| !! | AAAAGACAAGAGGGCACTTA+AGG | + | chr1.2:75192331-75192350 | MS.gene34149:CDS | 40.0% |
| AACCTCATTAGAGAGGTTGC+TGG | + | chr1.2:75192263-75192282 | MS.gene34149:CDS | 45.0% | |
| ACCTCATTAGAGAGGTTGCT+GGG | + | chr1.2:75192264-75192283 | MS.gene34149:CDS | 45.0% | |
| ACGTGCAAAGAAGAAGAGAG+AGG | + | chr1.2:75192382-75192401 | MS.gene34149:CDS | 45.0% | |
| AGGAGATGTCTAATGTCCTC+AGG | + | chr1.2:75192402-75192421 | MS.gene34149:CDS | 45.0% | |
| ATCACTGAGTTGCTCAAGGT+TGG | + | chr1.2:75192308-75192327 | MS.gene34149:CDS | 45.0% | |
| CCAACGAACAAACCAGTACT+TGG | - | chr1.2:75191717-75191736 | None:intergenic | 45.0% | |
| CTAATGTCCTCAGGAAGATG+AGG | + | chr1.2:75192411-75192430 | MS.gene34149:CDS | 45.0% | |
| GAACAAACCTCATCTTCCTG+AGG | - | chr1.2:75192421-75192440 | None:intergenic | 45.0% | |
| GCTCCTAAACAACCAAGTAC+TGG | + | chr1.2:75191702-75191721 | MS.gene34149:CDS | 45.0% | |
| TCAGTGATACGCTTCTCGTA+AGG | - | chr1.2:75192296-75192315 | None:intergenic | 45.0% | |
| TGTGAGGAACCTCATTAGAG+AGG | + | chr1.2:75192256-75192275 | MS.gene34149:CDS | 45.0% | |
| !! | CCAAGTACTGGTTTGTTCGT+TGG | + | chr1.2:75191714-75191733 | MS.gene34149:CDS | 45.0% |
| ACCCAGCAACCTCTCTAATG+AGG | - | chr1.2:75192268-75192287 | None:intergenic | 50.0% | |
| AGGCCACATCGTCACAAAGA+AGG | + | chr1.2:75191746-75191765 | MS.gene34149:CDS | 50.0% | |
| GCGTATCACTGAGTTGCTCA+AGG | + | chr1.2:75192304-75192323 | MS.gene34149:CDS | 50.0% | |
| ! | TTTTCAATGTGCAGGGCTGG+TGG | + | chr1.2:75192537-75192556 | MS.gene34149:intron | 50.0% |
| ! | GCAAGAGGGTGCACTTTGTG+AGG | + | chr1.2:75192240-75192259 | MS.gene34149:CDS | 55.0% |
| ACGTCCTTCAGCTCGCAAGG+GGG | + | chr1.2:75191779-75191798 | MS.gene34149:CDS | 60.0% | |
| CACGTCCTTCAGCTCGCAAG+GGG | + | chr1.2:75191778-75191797 | MS.gene34149:CDS | 60.0% | |
| CCACGTCCTTCAGCTCGCAA+GGG | + | chr1.2:75191777-75191796 | MS.gene34149:CDS | 60.0% | |
| TCCACGTCCTTCAGCTCGCA+AGG | + | chr1.2:75191776-75191795 | MS.gene34149:CDS | 60.0% | |
| ! | TCAATGTGCAGGGCTGGTGG+TGG | + | chr1.2:75192540-75192559 | MS.gene34149:intron | 60.0% |
| CCCTTGCGAGCTGAAGGACG+TGG | - | chr1.2:75191780-75191799 | None:intergenic | 65.0% | |
| CTGACCCCCTTGCGAGCTGA+AGG | - | chr1.2:75191786-75191805 | None:intergenic | 65.0% | |
| !! | TGCAGGGCTGGTGGTGGAGC+AGG | + | chr1.2:75192546-75192565 | MS.gene34149:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 75191699 | 75192584 | 75191699 | ID=MS.gene34149 |
| chr1.2 | mRNA | 75191699 | 75192584 | 75191699 | ID=MS.gene34149.t1;Parent=MS.gene34149 |
| chr1.2 | exon | 75191699 | 75191800 | 75191699 | ID=MS.gene34149.t1.exon1;Parent=MS.gene34149.t1 |
| chr1.2 | CDS | 75191699 | 75191800 | 75191699 | ID=cds.MS.gene34149.t1;Parent=MS.gene34149.t1 |
| chr1.2 | exon | 75192233 | 75192432 | 75192233 | ID=MS.gene34149.t1.exon2;Parent=MS.gene34149.t1 |
| chr1.2 | CDS | 75192233 | 75192432 | 75192233 | ID=cds.MS.gene34149.t1;Parent=MS.gene34149.t1 |
| chr1.2 | exon | 75192551 | 75192584 | 75192551 | ID=MS.gene34149.t1.exon3;Parent=MS.gene34149.t1 |
| chr1.2 | CDS | 75192551 | 75192584 | 75192551 | ID=cds.MS.gene34149.t1;Parent=MS.gene34149.t1 |
| Gene Sequence |
| Protein sequence |
