AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81493


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81493.t1 MTR_1g100960 96.396 111 3 1 1 110 1 111 3.83e-71 207
MS.gene81493.t1 MTR_6g053730 98.198 111 1 1 1 110 1 111 9.95e-71 206
MS.gene81493.t1 MTR_1g100960 97.030 101 3 0 1 101 1 101 9.27e-67 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81493.t1 AT3G53740 80.909 110 21 0 1 110 1 110 7.51e-58 174
MS.gene81493.t1 AT3G53740 80.909 110 21 0 1 110 1 110 7.51e-58 174
MS.gene81493.t1 AT3G53740 80.909 110 21 0 1 110 1 110 7.51e-58 174
MS.gene81493.t1 AT5G02450 82.692 104 18 0 7 110 3 106 3.67e-56 169
MS.gene81493.t1 AT2G37600 83.654 104 16 1 8 110 8 111 1.77e-55 168
MS.gene81493.t1 AT2G37600 83.654 104 16 1 8 110 8 111 1.77e-55 168
MS.gene81493.t1 AT3G53740 72.727 110 21 1 1 110 1 101 1.05e-48 150

Find 33 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCTTCTTGGCACGTTTA+TGG 0.140365 6.4:-20980908 None:intergenic
CTTCTTCTTGGCACGTTTAT+GGG 0.210182 6.4:-20980907 None:intergenic
CATCTCCTCACGCTTCTTCT+TGG 0.294580 6.4:-20980919 None:intergenic
CCTTTACGGCTAGAGGGTTT+AGG 0.305250 6.4:-20979217 None:intergenic
TTGCGCAGGGCTGGTGGAGC+AGG 0.374917 6.4:+20981066 MS.gene81493:intron
CTAAACCCTCTAGCCGTAAA+GGG 0.402747 6.4:+20979218 MS.gene81493:CDS
ATGGGTTCCGAGCTTTCTCT+TGG 0.449681 6.4:-20980889 None:intergenic
GGCGCCGAAGCAGCCAAGTA+CGG 0.452682 6.4:+20979141 MS.gene81493:CDS
GAAGAGCAAACCTCATCTTT+CGG 0.452731 6.4:-20980953 None:intergenic
AACCTCATCCGCGAGGTAGC+TGG 0.486512 6.4:+20980795 MS.gene81493:CDS
CCTAAACCCTCTAGCCGTAA+AGG 0.488599 6.4:+20979217 MS.gene81493:CDS
AGACCCGTACTTGGCTGCTT+CGG 0.489391 6.4:-20979145 None:intergenic
GCGCCGAAGCAGCCAAGTAC+GGG 0.490664 6.4:+20979142 MS.gene81493:CDS
ATCACTGAATTGCTGAAGGT+TGG 0.491376 6.4:+20980840 MS.gene81493:CDS
TGAAGGTTGGAAAGGATAAG+AGG 0.498071 6.4:+20980853 MS.gene81493:CDS
GCAAGCGTGTGCACTTTGTA+AGG 0.505489 6.4:+20980772 MS.gene81493:CDS
AGCCAGCTACCTCGCGGATG+AGG 0.509753 6.4:-20980797 None:intergenic
TCAGTGATACGCTTCTCATA+AGG 0.516197 6.4:-20980825 None:intergenic
TCATACCCCTTTACGGCTAG+AGG 0.525898 6.4:-20979224 None:intergenic
TGTTGTTACTAAGAAGGAGT+TGG 0.545489 6.4:+20979192 MS.gene81493:CDS
GGGTCATGTTGTTACTAAGA+AGG 0.556387 6.4:+20979186 MS.gene81493:CDS
TGAATTGCTGAAGGTTGGAA+AGG 0.563716 6.4:+20980845 MS.gene81493:CDS
AAAGTTGCCAAGAGAAAGCT+CGG 0.571574 6.4:+20980882 MS.gene81493:CDS
CATACCCCTTTACGGCTAGA+GGG 0.582186 6.4:-20979223 None:intergenic
CCTCATCTTTCGGAGGACGT+TGG 0.608911 6.4:-20980943 None:intergenic
GAAGGTTGGAAAGGATAAGA+GGG 0.613741 6.4:+20980854 MS.gene81493:CDS
GCGTATCACTGAATTGCTGA+AGG 0.627654 6.4:+20980836 MS.gene81493:CDS
CCAACGTCCTCCGAAAGATG+AGG 0.657638 6.4:+20980943 MS.gene81493:CDS
TAAACCCTCTAGCCGTAAAG+GGG 0.659101 6.4:+20979219 MS.gene81493:CDS
GAGCAAACCTCATCTTTCGG+AGG 0.683152 6.4:-20980950 None:intergenic
GTGCAAAGCCAGCTACCTCG+CGG 0.704628 6.4:-20980803 None:intergenic
ACGTGCCAAGAAGAAGCGTG+AGG 0.711062 6.4:+20980914 MS.gene81493:CDS
TGTAAGGAACCTCATCCGCG+AGG 0.767870 6.4:+20980788 MS.gene81493:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAAAAGACATTATTT+GGG + chr6.4:20980539-20980558 MS.gene81493:intron 10.0%
!! CTTATTTAAAAGACATTATT+TGG + chr6.4:20980538-20980557 MS.gene81493:intron 15.0%
!! TAAGAAAATTAACGAAAATA+TGG - chr6.4:20980522-20980541 None:intergenic 15.0%
!!! AGAGTAAAATTAAATTGTTT+TGG + chr6.4:20980086-20980105 MS.gene81493:intron 15.0%
!!! GGTATGATTTTTTATTTTTA+GGG + chr6.4:20979240-20979259 MS.gene81493:intron 15.0%
!!! TTATTATTAAACTTGTTACT+TGG + chr6.4:20981010-20981029 MS.gene81493:intron 15.0%
!!! TTCAAGTTTAGTTAATTTTT+GGG + chr6.4:20979700-20979719 MS.gene81493:intron 15.0%
!!! TTTAGGTTTAGTTAATTTTT+GGG + chr6.4:20979347-20979366 MS.gene81493:intron 15.0%
!!! TTTCAAGTTTAGTTAATTTT+TGG + chr6.4:20979699-20979718 MS.gene81493:intron 15.0%
!!! TTTTAATTTTCATTTGTGTT+TGG + chr6.4:20979394-20979413 MS.gene81493:intron 15.0%
!!! TTTTAGGTTTAGTTAATTTT+TGG + chr6.4:20979346-20979365 MS.gene81493:intron 15.0%
!! AAGTTTAATAATAAAACACC+TGG - chr6.4:20981004-20981023 None:intergenic 20.0%
!! AATGAAATTGTTGATATTCT+GGG + chr6.4:20980683-20980702 MS.gene81493:intron 20.0%
!! AGTTTAATAATAAAACACCT+GGG - chr6.4:20981003-20981022 None:intergenic 20.0%
!! ATAGTACTATGTAATATATC+TGG + chr6.4:20980570-20980589 MS.gene81493:intron 20.0%
!! CAATAATCTGAGTAAATTAA+TGG + chr6.4:20979454-20979473 MS.gene81493:intron 20.0%
!! GTTATTAATTGTAATAGTGA+AGG + chr6.4:20979782-20979801 MS.gene81493:intron 20.0%
!! TAATGAAATTGTTGATATTC+TGG + chr6.4:20980682-20980701 MS.gene81493:intron 20.0%
!! TCACTAATATTTAAATACCT+TGG - chr6.4:20979623-20979642 None:intergenic 20.0%
!!! AGTTTAGTTTTAAGTTTTGT+TGG + chr6.4:20979887-20979906 MS.gene81493:intron 20.0%
!!! ATTGTTCATTTTTCATCTAA+TGG - chr6.4:20979966-20979985 None:intergenic 20.0%
!!! GAGGAATTTTTTTTATAGTT+AGG + chr6.4:20979987-20980006 MS.gene81493:intron 20.0%
!!! GATAAATGAGAATCTATTTT+AGG + chr6.4:20979330-20979349 MS.gene81493:intron 20.0%
!!! GGGTATGATTTTTTATTTTT+AGG + chr6.4:20979239-20979258 MS.gene81493:intron 20.0%
!!! TGAAACTATTTTGTGATTTT+TGG + chr6.4:20980372-20980391 MS.gene81493:intron 20.0%
!!! TTTACAATAACATGGAATTT+TGG + chr6.4:20980320-20980339 MS.gene81493:intron 20.0%
!!! TTTTGATTTTGGTTAATGTT+TGG + chr6.4:20979650-20979669 MS.gene81493:intron 20.0%
! AATAAGTTGTTTATCCAAAG+AGG - chr6.4:20980014-20980033 None:intergenic 25.0%
! ACATCAAATCACATAACAAA+TGG - chr6.4:20980272-20980291 None:intergenic 25.0%
! AGATGAAAAATGAACAATTG+AGG + chr6.4:20979968-20979987 MS.gene81493:intron 25.0%
! AGTTTCAATTGAAATCCAAA+GGG - chr6.4:20980359-20980378 None:intergenic 25.0%
! ATAAGTTGTTTATCCAAAGA+GGG - chr6.4:20980013-20980032 None:intergenic 25.0%
! TAAAAAATCATACCCCTTTA+CGG - chr6.4:20979234-20979253 None:intergenic 25.0%
! TAACTCAAATACATTCAATG+AGG - chr6.4:20980460-20980479 None:intergenic 25.0%
! TAGTTTCAATTGAAATCCAA+AGG - chr6.4:20980360-20980379 None:intergenic 25.0%
! TTATCATTGATACTGTTCTT+TGG + chr6.4:20980711-20980730 MS.gene81493:intron 25.0%
!! ATATATCTGGAGTCAAAATA+TGG + chr6.4:20980583-20980602 MS.gene81493:intron 25.0%
!! GTTATTGTTCATTTTGTGTT+TGG + chr6.4:20979308-20979327 MS.gene81493:intron 25.0%
!! TTTGGTTAATGTTTGGAATT+TGG + chr6.4:20979657-20979676 MS.gene81493:intron 25.0%
!!! CTATTTTGTGATTTTTGGAA+TGG + chr6.4:20980377-20980396 MS.gene81493:intron 25.0%
!!! GTTTTTTATTGAAAATGCCA+AGG + chr6.4:20979603-20979622 MS.gene81493:intron 25.0%
!!! TATCTTTTTAATTTTGCGCA+GGG + chr6.4:20981053-20981072 MS.gene81493:intron 25.0%
!!! TTAATTTTTGGGTATAGTGA+TGG + chr6.4:20979358-20979377 MS.gene81493:intron 25.0%
!!! TTTTGTTTATTATACATCCC+AGG + chr6.4:20980983-20981002 MS.gene81493:intron 25.0%
ATATGAAAATGCAACAGCTT+TGG + chr6.4:20980485-20980504 MS.gene81493:intron 30.0%
TTAGATATTAGTGATGCGTT+TGG + chr6.4:20979264-20979283 MS.gene81493:intron 30.0%
! AAGCTGTTGCATTTTCATAT+AGG - chr6.4:20980485-20980504 None:intergenic 30.0%
! CGTCTTTTGAGCTAATTTTT+GGG + chr6.4:20979526-20979545 MS.gene81493:intron 30.0%
! CTTTTTGTTGGATTGAACAA+GGG + chr6.4:20979166-20979185 MS.gene81493:CDS 30.0%
! TCGTCTTTTGAGCTAATTTT+TGG + chr6.4:20979525-20979544 MS.gene81493:intron 30.0%
! TCTTTTTGTTGGATTGAACA+AGG + chr6.4:20979165-20979184 MS.gene81493:CDS 30.0%
!!! CTATCTTTTTAATTTTGCGC+AGG + chr6.4:20981052-20981071 MS.gene81493:intron 30.0%
!!! GTGACTGTTTGTTTTGATTT+TGG + chr6.4:20979639-20979658 MS.gene81493:intron 30.0%
TCAAATAAGCTCATCCAAAC+AGG + chr6.4:20980246-20980265 MS.gene81493:intron 35.0%
TGTTGTTACTAAGAAGGAGT+TGG + chr6.4:20979192-20979211 MS.gene81493:CDS 35.0%
! CATTTTCGTTGCAATGCAAA+TGG + chr6.4:20979933-20979952 MS.gene81493:intron 35.0%
! GGCACTGTTTTACAATAACA+TGG + chr6.4:20980312-20980331 MS.gene81493:intron 35.0%
! GTTTAGATTTTGCGTTGTGT+TGG + chr6.4:20979833-20979852 MS.gene81493:intron 35.0%
! TTTTATAGTTAGGCCCTCTT+TGG + chr6.4:20979997-20980016 MS.gene81493:intron 35.0%
AAAGTTGCCAAGAGAAAGCT+CGG + chr6.4:20980882-20980901 MS.gene81493:CDS 40.0%
AGATCGTTTGTGAGACTGTT+TGG - chr6.4:20980210-20980229 None:intergenic 40.0%
ATCACTGAATTGCTGAAGGT+TGG + chr6.4:20980840-20980859 MS.gene81493:CDS 40.0%
CACATAACAAATGGCCTGTT+TGG - chr6.4:20980263-20980282 None:intergenic 40.0%
CTTCTTCTTGGCACGTTTAT+GGG - chr6.4:20980910-20980929 None:intergenic 40.0%
GAAGAGCAAACCTCATCTTT+CGG - chr6.4:20980956-20980975 None:intergenic 40.0%
GAAGGTTGGAAAGGATAAGA+GGG + chr6.4:20980854-20980873 MS.gene81493:CDS 40.0%
GATCGTTTGTGAGACTGTTT+GGG - chr6.4:20980209-20980228 None:intergenic 40.0%
GGGTCATGTTGTTACTAAGA+AGG + chr6.4:20979186-20979205 MS.gene81493:CDS 40.0%
TCAGTGATACGCTTCTCATA+AGG - chr6.4:20980828-20980847 None:intergenic 40.0%
TGAAGGTTGGAAAGGATAAG+AGG + chr6.4:20980853-20980872 MS.gene81493:CDS 40.0%
TGAATTGCTGAAGGTTGGAA+AGG + chr6.4:20980845-20980864 MS.gene81493:CDS 40.0%
TTGGTCCTAGTCCTAATATG+CGG + chr6.4:20980730-20980749 MS.gene81493:intron 40.0%
! TGAGTTGAGTTGTAGAATGC+AGG + chr6.4:20980291-20980310 MS.gene81493:intron 40.0%
!! GCTGTTTTCATGAGCTGTTT+TGG + chr6.4:20980121-20980140 MS.gene81493:intron 40.0%
!!! GAGCTAATTTTTGGGTTCAG+AGG + chr6.4:20979534-20979553 MS.gene81493:intron 40.0%
!!! GCTTGCTAGTTTTCTGTTCA+AGG - chr6.4:20980758-20980777 None:intergenic 40.0%
!!! GGAGTCTATTTTTTGCCCTT+TGG + chr6.4:20980341-20980360 MS.gene81493:intron 40.0%
!!! TTTTTAATTTTGCGCAGGGC+TGG + chr6.4:20981057-20981076 MS.gene81493:intron 40.0%
CCAACAAAAAGACCCGTACT+TGG - chr6.4:20979157-20979176 None:intergenic 45.0%
CTAAACCCTCTAGCCGTAAA+GGG + chr6.4:20979218-20979237 MS.gene81493:CDS 45.0%
GCGTATCACTGAATTGCTGA+AGG + chr6.4:20980836-20980855 MS.gene81493:CDS 45.0%
GCTTCTTCTTGGCACGTTTA+TGG - chr6.4:20980911-20980930 None:intergenic 45.0%
TAAACCCTCTAGCCGTAAAG+GGG + chr6.4:20979219-20979238 MS.gene81493:CDS 45.0%
! CCAAGTACGGGTCTTTTTGT+TGG + chr6.4:20979154-20979173 MS.gene81493:CDS 45.0%
!! TGTTCAAGGCACCGCATATT+AGG - chr6.4:20980744-20980763 None:intergenic 45.0%
ATGGGTTCCGAGCTTTCTCT+TGG - chr6.4:20980892-20980911 None:intergenic 50.0%
CATACCCCTTTACGGCTAGA+GGG - chr6.4:20979226-20979245 None:intergenic 50.0%
CATCTCCTCACGCTTCTTCT+TGG - chr6.4:20980922-20980941 None:intergenic 50.0%
CCTAAACCCTCTAGCCGTAA+AGG + chr6.4:20979217-20979236 MS.gene81493:CDS 50.0%
CCTTTACGGCTAGAGGGTTT+AGG - chr6.4:20979220-20979239 None:intergenic 50.0%
GAGCAAACCTCATCTTTCGG+AGG - chr6.4:20980953-20980972 None:intergenic 50.0%
GCAAGCGTGTGCACTTTGTA+AGG + chr6.4:20980772-20980791 MS.gene81493:CDS 50.0%
TCATACCCCTTTACGGCTAG+AGG - chr6.4:20979227-20979246 None:intergenic 50.0%
! AGGCACCGCATATTAGGACT+AGG - chr6.4:20980738-20980757 None:intergenic 50.0%
! TTAATTTTGCGCAGGGCTGG+TGG + chr6.4:20981060-20981079 MS.gene81493:intron 50.0%
AGACCCGTACTTGGCTGCTT+CGG - chr6.4:20979148-20979167 None:intergenic 55.0%
CCAACGTCCTCCGAAAGATG+AGG + chr6.4:20980943-20980962 MS.gene81493:CDS 55.0%
CCTCATCTTTCGGAGGACGT+TGG - chr6.4:20980946-20980965 None:intergenic 55.0%
TGTAAGGAACCTCATCCGCG+AGG + chr6.4:20980788-20980807 MS.gene81493:CDS 55.0%
! ACGTGCCAAGAAGAAGCGTG+AGG + chr6.4:20980914-20980933 MS.gene81493:CDS 55.0%
AACCTCATCCGCGAGGTAGC+TGG + chr6.4:20980795-20980814 MS.gene81493:CDS 60.0%
GTGCAAAGCCAGCTACCTCG+CGG - chr6.4:20980806-20980825 None:intergenic 60.0%
AGCCAGCTACCTCGCGGATG+AGG - chr6.4:20980800-20980819 None:intergenic 65.0%
GCGCCGAAGCAGCCAAGTAC+GGG + chr6.4:20979142-20979161 MS.gene81493:CDS 65.0%
! GGCGCCGAAGCAGCCAAGTA+CGG + chr6.4:20979141-20979160 MS.gene81493:CDS 65.0%
! TTGCGCAGGGCTGGTGGAGC+AGG + chr6.4:20981066-20981085 MS.gene81493:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 20979139 20981104 20979139 ID=MS.gene81493
chr6.4 mRNA 20979139 20981104 20979139 ID=MS.gene81493.t1;Parent=MS.gene81493
chr6.4 exon 20979139 20979240 20979139 ID=MS.gene81493.t1.exon1;Parent=MS.gene81493.t1
chr6.4 CDS 20979139 20979240 20979139 ID=cds.MS.gene81493.t1;Parent=MS.gene81493.t1
chr6.4 exon 20980765 20980964 20980765 ID=MS.gene81493.t1.exon2;Parent=MS.gene81493.t1
chr6.4 CDS 20980765 20980964 20980765 ID=cds.MS.gene81493.t1;Parent=MS.gene81493.t1
chr6.4 exon 20981074 20981104 20981074 ID=MS.gene81493.t1.exon3;Parent=MS.gene81493.t1
chr6.4 CDS 20981074 20981104 20981074 ID=cds.MS.gene81493.t1;Parent=MS.gene81493.t1
Gene Sequence

>MS.gene81493

ATGGCGCCGAAGCAGCCAAGTACGGGTCTTTTTGTTGGATTGAACAAGGGTCATGTTGTTACTAAGAAGGAGTTGGCTCCTAAACCCTCTAGCCGTAAAGGGGTATGATTTTTTATTTTTAGGGTTTAGATATTAGTGATGCGTTTGGATTGTTTAGATTTTTAGTTATGTTATTGTTCATTTTGTGTTTGGATAAATGAGAATCTATTTTAGGTTTAGTTAATTTTTGGGTATAGTGATGGCTTTCATTGTTTGTTTTAATTTTCATTTGTGTTTGGATAATCAGATTAGTGATGAATTTGCAATAAGCAAATGCAATAATCTGAGTAAATTAATGGTTGATTTTATTTTCTGTTTTGATCTTTTAAGTGATTTTATTGTCTGTTTCGTCTTTTGAGCTAATTTTTGGGTTCAGAGGTTAGTAATGCATTTGTATTGTTCAGATTTTGAATTTTTTTAGTGATGTTTTTTATTGAAAATGCCAAGGTATTTAAATATTAGTGACTGTTTGTTTTGATTTTGGTTAATGTTTGGAATTTGGATTTACATTTGAGAATTTATTTCAAGTTTAGTTAATTTTTGGGTATAGAGATTAGTGATGCATTTGCAAGTTCTATTAAGATTTTTAATTATTTTGAGTGATGTTATTAATTGTAATAGTGAAGGTAATCAAATATAGTGATTTTTGTTGTCTGTTTAGATTTTGCGTTGTGTTGGAAATTTTGTTTATGTATGAAGATTTATTTGAAGTTTAGTTTTAAGTTTTGTTGGATTTGTTAATTAGAGATTGAAATCATTTTCGTTGCAATGCAAATGGTTTACTACCATTAGATGAAAAATGAACAATTGAGGAATTTTTTTTATAGTTAGGCCCTCTTTGGATAAACAACTTATTTTCAGCTTCGAGCATAAGTGCTTCTGCATAAGCTATTTCTATAGCAAGAGATAGAGTAAAATTAAATTGTTTTGGTAAAAGCTATAAGCTGTTTTCATGAGCTGTTTTGGAGAACTTAAGAGAATAAGCTTATCAGCTTATAAACATGACATTAACTATTTTCATAAGTTATCCCAAACAGTCTCACAAACGATCTTGTTGAGTAGATAAGATCAAATAAGCTCATCCAAACAGGCCATTTGTTATGTGATTTGATGTGAGTTGAGTTGTAGAATGCAGGCACTGTTTTACAATAACATGGAATTTTGGAGTCTATTTTTTGCCCTTTGGATTTCAATTGAAACTATTTTGTGATTTTTGGAATGGCTTACGTAATATGTTAAACTATAGTGCTAGTTGTTGCAAAGATTTTGTGAAATGCTGCCTCATTGAATGTATTTGAGTTACTCCTATATGAAAATGCAACAGCTTTGGTTCATAACAATCCATATTTTCGTTAATTTTCTTATTTAAAAGACATTATTTGGGTTGCTACTATAGTACTATGTAATATATCTGGAGTCAAAATATGGCTGAAGTTTGTTGATTTGAATAAAAATGACGTTGAATAGATATTGACGCTTAATGAAAGATATAAAATACAGAAAATAATGAAATTGTTGATATTCTGGGCTTGCTTATCATTGATACTGTTCTTTGGTCCTAGTCCTAATATGCGGTGCCTTGAACAGAAAACTAGCAAGCGTGTGCACTTTGTAAGGAACCTCATCCGCGAGGTAGCTGGCTTTGCACCTTATGAGAAGCGTATCACTGAATTGCTGAAGGTTGGAAAGGATAAGAGGGCACTGAAAGTTGCCAAGAGAAAGCTCGGAACCCATAAACGTGCCAAGAAGAAGCGTGAGGAGATGTCCAACGTCCTCCGAAAGATGAGGTTTGCTCTTCATTCTTTTTTTGTTTATTATACATCCCAGGTGTTTTATTATTAAACTTGTTACTTGGTGAAATTTGAGTTCTTATGCTATCTTTTTAATTTTGCGCAGGGCTGGTGGAGCAGGCGACAAGAAGAAATAA

Protein sequence

>MS.gene81493.t1

MAPKQPSTGLFVGLNKGHVVTKKELAPKPSSRKGKTSKRVHFVRNLIREVAGFAPYEKRITELLKVGKDKRALKVAKRKLGTHKRAKKKREEMSNVLRKMRAGGAGDKKK