Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061880.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061880.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene061880 | 0.845663 | 3.53E-59 | -1.69E-46 |
| MS.gene050584 | MS.gene061880 | 0.855152 | 7.64E-62 | -1.69E-46 |
| MS.gene050585 | MS.gene061880 | 0.837628 | 4.65E-57 | -1.69E-46 |
| MS.gene050587 | MS.gene061880 | 0.809234 | 2.09E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene061880 | 0.82157 | 3.76E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene061880 | 0.802306 | 5.97E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene061880 | 0.830672 | 2.57E-55 | -1.69E-46 |
| MS.gene051789 | MS.gene061880 | 0.872372 | 3.35E-67 | -1.69E-46 |
| MS.gene052142 | MS.gene061880 | 0.824592 | 7.43E-54 | -1.69E-46 |
| MS.gene052665 | MS.gene061880 | 0.802543 | 5.34E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene061880 | 0.843584 | 1.28E-58 | -1.69E-46 |
| MS.gene053383 | MS.gene061880 | 0.809966 | 1.46E-50 | -1.69E-46 |
| MS.gene053563 | MS.gene061880 | 0.816781 | 4.63E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene061880 | 0.858666 | 7.04E-63 | -1.69E-46 |
| MS.gene054596 | MS.gene061880 | 0.809088 | 2.25E-50 | -1.69E-46 |
| MS.gene055359 | MS.gene061880 | 0.821805 | 3.32E-53 | -1.69E-46 |
| MS.gene058168 | MS.gene061880 | 0.80814 | 3.58E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene061880 | 0.869632 | 2.68E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061880.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene061880.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGTGGACTTGGTGGTTT+GGG | 0.209445 | 7.4:-10567662 | MS.gene061880:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.4:-10567758 | MS.gene061880:CDS |
| GGAGGACTTGGTGGGGTTGG+TGG | 0.235350 | 7.4:-10567641 | MS.gene061880:CDS |
| CCTAGGTGGACTTGGTGGTT+TGG | 0.244833 | 7.4:-10567663 | MS.gene061880:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.4:-10567653 | MS.gene061880:CDS |
| GGACTTGGTGGGGTTGGTGG+TGG | 0.293034 | 7.4:-10567638 | MS.gene061880:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.4:-10567620 | MS.gene061880:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.4:-10567698 | MS.gene061880:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.314590 | 7.4:-10567650 | MS.gene061880:CDS |
| TTGGGAGGACTTGGTGGGGT+TGG | 0.314725 | 7.4:-10567644 | MS.gene061880:CDS |
| GGTGGGGTTGGTGGTGGAGC+TGG | 0.316689 | 7.4:-10567632 | MS.gene061880:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.321018 | 7.4:-10567626 | MS.gene061880:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.4:-10567731 | MS.gene061880:CDS |
| TTAGGTGGTGGTGGTGGCCT+AGG | 0.323708 | 7.4:-10567680 | MS.gene061880:CDS |
| GGTGGTGGCCTAGGTGGACT+TGG | 0.331166 | 7.4:-10567671 | MS.gene061880:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.4:-10567608 | MS.gene061880:CDS |
| GGGGTTGGTGGTGGAGCTGG+TGG | 0.333006 | 7.4:-10567629 | MS.gene061880:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.4:-10567752 | MS.gene061880:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.4:-10567602 | MS.gene061880:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.4:+10567774 | None:intergenic |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.4:-10567614 | MS.gene061880:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.4:-10567770 | MS.gene061880:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.4:-10567617 | MS.gene061880:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.4:-10567596 | MS.gene061880:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.4:-10567710 | MS.gene061880:CDS |
| GTGGTTTGGGAGGACTTGGT+GGG | 0.431797 | 7.4:-10567649 | MS.gene061880:CDS |
| TGGTTTGGGAGGACTTGGTG+GGG | 0.448017 | 7.4:-10567648 | MS.gene061880:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.4:-10567593 | MS.gene061880:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.4:-10567861 | MS.gene061880:CDS |
| GGTGGCCTAGGTGGACTTGG+TGG | 0.480801 | 7.4:-10567668 | MS.gene061880:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.508576 | 7.4:-10567659 | MS.gene061880:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.4:-10567843 | MS.gene061880:CDS |
| GGTGGTGGTGGTGGCCTAGG+TGG | 0.516203 | 7.4:-10567677 | MS.gene061880:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.4:-10567798 | MS.gene061880:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.527759 | 7.4:-10567686 | MS.gene061880:CDS |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.4:-10567692 | MS.gene061880:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.4:+10567716 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.4:-10567707 | MS.gene061880:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.4:-10567689 | MS.gene061880:CDS |
| GAATACTGATCTTAAGGGAA+AGG | 0.564229 | 7.4:+10567563 | None:intergenic |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.4:-10567806 | MS.gene061880:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.4:-10567743 | MS.gene061880:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.4:-10567695 | MS.gene061880:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.4:-10567877 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.4:-10567767 | MS.gene061880:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.4:+10567797 | None:intergenic |
| CCAAACCACCAAGTCCACCT+AGG | 0.637542 | 7.4:+10567663 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | - | chr7.4:10567697-10567716 | MS.gene061880:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | - | chr7.4:10567579-10567598 | MS.gene061880:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | - | chr7.4:10567670-10567689 | MS.gene061880:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | + | chr7.4:10567669-10567688 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | - | chr7.4:10567634-10567653 | MS.gene061880:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | - | chr7.4:10567673-10567692 | MS.gene061880:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | - | chr7.4:10567682-10567701 | MS.gene061880:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | - | chr7.4:10567712-10567731 | MS.gene061880:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | - | chr7.4:10567709-10567728 | MS.gene061880:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | - | chr7.4:10567688-10567707 | MS.gene061880:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | + | chr7.4:10567646-10567665 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | - | chr7.4:10567721-10567740 | MS.gene061880:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | - | chr7.4:10567718-10567737 | MS.gene061880:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | - | chr7.4:10567597-10567616 | MS.gene061880:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | - | chr7.4:10567642-10567661 | MS.gene061880:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | - | chr7.4:10567748-10567767 | MS.gene061880:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | + | chr7.4:10567727-10567746 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | - | chr7.4:10567745-10567764 | MS.gene061880:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | - | chr7.4:10567742-10567761 | MS.gene061880:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | - | chr7.4:10567730-10567749 | MS.gene061880:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | - | chr7.4:10567724-10567743 | MS.gene061880:CDS | 50.0% |
| !! | CTAGGTGGACTTGGTGGTTT+GGG | - | chr7.4:10567778-10567797 | MS.gene061880:CDS | 50.0% |
| CCAAACCACCAAGTCCACCT+AGG | + | chr7.4:10567780-10567799 | None:intergenic | 55.0% | |
| GGAATTGGAGGTGGAATCGG+TGG | - | chr7.4:10567733-10567752 | MS.gene061880:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | - | chr7.4:10567787-10567806 | MS.gene061880:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | - | chr7.4:10567751-10567770 | MS.gene061880:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | - | chr7.4:10567844-10567863 | MS.gene061880:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | - | chr7.4:10567847-10567866 | MS.gene061880:CDS | 55.0% |
| ! | GTGGTTTGGGAGGACTTGGT+GGG | - | chr7.4:10567791-10567810 | MS.gene061880:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | - | chr7.4:10567838-10567857 | MS.gene061880:CDS | 55.0% |
| !! | CCTAGGTGGACTTGGTGGTT+TGG | - | chr7.4:10567777-10567796 | MS.gene061880:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | - | chr7.4:10567754-10567773 | MS.gene061880:CDS | 55.0% |
| !! | TGGTTTGGGAGGACTTGGTG+GGG | - | chr7.4:10567792-10567811 | MS.gene061880:CDS | 55.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | - | chr7.4:10567832-10567851 | MS.gene061880:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | - | chr7.4:10567790-10567809 | MS.gene061880:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | - | chr7.4:10567781-10567800 | MS.gene061880:CDS | 60.0% |
| !! | TTAGGTGGTGGTGGTGGCCT+AGG | - | chr7.4:10567760-10567779 | MS.gene061880:CDS | 60.0% |
| !! | TTGGGAGGACTTGGTGGGGT+TGG | - | chr7.4:10567796-10567815 | MS.gene061880:CDS | 60.0% |
| ! | GGTGGCCTAGGTGGACTTGG+TGG | - | chr7.4:10567772-10567791 | MS.gene061880:CDS | 65.0% |
| ! | GGTGGTGGCCTAGGTGGACT+TGG | - | chr7.4:10567769-10567788 | MS.gene061880:CDS | 65.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | - | chr7.4:10567826-10567845 | MS.gene061880:CDS | 65.0% |
| !! | GGACTTGGTGGGGTTGGTGG+TGG | - | chr7.4:10567802-10567821 | MS.gene061880:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | - | chr7.4:10567823-10567842 | MS.gene061880:CDS | 65.0% |
| !! | GGAGGACTTGGTGGGGTTGG+TGG | - | chr7.4:10567799-10567818 | MS.gene061880:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | - | chr7.4:10567820-10567839 | MS.gene061880:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | - | chr7.4:10567814-10567833 | MS.gene061880:CDS | 65.0% |
| !! | GGGGTTGGTGGTGGAGCTGG+TGG | - | chr7.4:10567811-10567830 | MS.gene061880:CDS | 70.0% |
| !! | GGTGGGGTTGGTGGTGGAGC+TGG | - | chr7.4:10567808-10567827 | MS.gene061880:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGCCTAGG+TGG | - | chr7.4:10567763-10567782 | MS.gene061880:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10567574 | 10567888 | 10567574 | ID=MS.gene061880 |
| chr7.4 | mRNA | 10567574 | 10567888 | 10567574 | ID=MS.gene061880.t1;Parent=MS.gene061880 |
| chr7.4 | exon | 10567574 | 10567888 | 10567574 | ID=MS.gene061880.t1.exon1;Parent=MS.gene061880.t1 |
| chr7.4 | CDS | 10567574 | 10567888 | 10567574 | ID=cds.MS.gene061880.t1;Parent=MS.gene061880.t1 |
| Gene Sequence |
| Protein sequence |
