Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062041.t1 | XP_013463689.1 | 100 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 5.60E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062041.t1 | B7FMQ0 | 100.0 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 4.1e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene062041 | 0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene062041 | 0.831654 | 1.48E-55 | -1.69E-46 |
| MS.gene050721 | MS.gene062041 | 0.832492 | 9.17E-56 | -1.69E-46 |
| MS.gene050724 | MS.gene062041 | 0.830488 | 2.86E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene062041 | 0.819111 | 1.38E-52 | -1.69E-46 |
| MS.gene051757 | MS.gene062041 | 0.812966 | 3.25E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene062041 | 0.816853 | 4.46E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene062041 | 0.808646 | 2.80E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene062041 | 0.815978 | 7.01E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene062041 | 0.817119 | 3.89E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene062041 | 0.802583 | 5.24E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene062041 | 0.800404 | 1.46E-48 | -1.69E-46 |
| MS.gene058906 | MS.gene062041 | 0.818409 | 1.99E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene062041 | 0.821447 | 4.02E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene062041 | 0.800068 | 1.71E-48 | -1.69E-46 |
| MS.gene060685 | MS.gene062041 | 0.802421 | 5.65E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062041.t1 | MTR_2g046160 | 100.000 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 7.90e-70 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062041.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene062041.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene062041.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene062041.t1 | AT5G57860 | 57.143 | 63 | 27 | 0 | 1 | 63 | 3 | 65 | 1.45e-22 | 83.2 |
Find 24 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAAAATTCTTGACATAA+AGG | 0.387433 | 2.3:+44603506 | MS.gene062041:CDS |
| TAAGACTCTGGCTGATCAAA+AGG | 0.457105 | 2.3:+44603611 | MS.gene062041:CDS |
| ATCAACCGTTGGTCCTTAGC+AGG | 0.459907 | 2.3:-44603555 | None:intergenic |
| TCCAGATGATAATGAGTTTG+AGG | 0.484717 | 2.3:+44604827 | MS.gene062041:intron |
| CGGTTGATTCTACCTGGTAC+AGG | 0.492432 | 2.3:+44603570 | MS.gene062041:CDS |
| CTCGTCTGATAGAAATCACT+TGG | 0.514868 | 2.3:-44604867 | None:intergenic |
| ATTAGACGACTCTAAGACTC+TGG | 0.517557 | 2.3:+44603599 | MS.gene062041:CDS |
| CCCAAGTTTACCTTTCCTCA+AGG | 0.539674 | 2.3:-44603869 | None:intergenic |
| TCTAATACTTCCCCTGTACC+AGG | 0.547045 | 2.3:-44603582 | None:intergenic |
| GCTTGTTGACAAACCTGCTA+AGG | 0.558954 | 2.3:+44603542 | MS.gene062041:CDS |
| GGTTGATTCTACCTGGTACA+GGG | 0.577903 | 2.3:+44603571 | MS.gene062041:CDS |
| AACATACTTCATCCGATGCA+AGG | 0.594573 | 2.3:+44603479 | MS.gene062041:CDS |
| CTGTTGTTGCACTGACCTTG+AGG | 0.605739 | 2.3:+44603854 | MS.gene062041:CDS |
| GACCAACGGTTGATTCTACC+TGG | 0.613786 | 2.3:+44603564 | MS.gene062041:CDS |
| CAAGTGATTTCTATCAGACG+AGG | 0.638963 | 2.3:+44604868 | MS.gene062041:CDS |
| AGATGATAATGAGTTTGAGG+AGG | 0.645303 | 2.3:+44604830 | MS.gene062041:intron |
| TATCAGACGAGGGACGCAGA+GGG | 0.646270 | 2.3:+44604879 | MS.gene062041:CDS |
| GTTGATTCTACCTGGTACAG+GGG | 0.650099 | 2.3:+44603572 | MS.gene062041:CDS |
| GGGACGCAGAGGGTGCCAGT+TGG | 0.652030 | 2.3:+44604889 | MS.gene062041:CDS |
| ACAAACCTGCTAAGGACCAA+CGG | 0.658865 | 2.3:+44603550 | MS.gene062041:CDS |
| TACCAGGTAGAATCAACCGT+TGG | 0.662250 | 2.3:-44603566 | None:intergenic |
| CTATCAGACGAGGGACGCAG+AGG | 0.671614 | 2.3:+44604878 | MS.gene062041:CDS |
| GTTGCACTGACCTTGAGGAA+AGG | 0.688680 | 2.3:+44603859 | MS.gene062041:CDS |
| AAGTGATTTCTATCAGACGA+GGG | 0.731271 | 2.3:+44604869 | MS.gene062041:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTTATGAATTTATATT+AGG | + | chr2.3:44603900-44603919 | MS.gene062041:intron | 10.0% |
| !! | TATAAATTCATAAATTTCTA+AGG | - | chr2.3:44603898-44603917 | None:intergenic | 10.0% |
| !!! | AAATTTAAAATTGTTGCATA+AGG | - | chr2.3:44604149-44604168 | None:intergenic | 15.0% |
| !!! | AATCTGAAATGTAAAAAAAA+TGG | - | chr2.3:44603824-44603843 | None:intergenic | 15.0% |
| !!! | TATTGATATCTTTAACTTTT+AGG | + | chr2.3:44604607-44604626 | MS.gene062041:intron | 15.0% |
| !!! | TCAATAAATTTGTTTTATCT+TGG | - | chr2.3:44604351-44604370 | None:intergenic | 15.0% |
| !!! | TTGTTTTAAAAGTTAACTTT+TGG | - | chr2.3:44604389-44604408 | None:intergenic | 15.0% |
| !! | AGTTATAAACTAAGAATCAA+TGG | - | chr2.3:44603741-44603760 | None:intergenic | 20.0% |
| !! | ATAATTGAAGAAATTCATGA+AGG | + | chr2.3:44604566-44604585 | MS.gene062041:intron | 20.0% |
| !! | GAATAAAGATAAACATACTT+AGG | - | chr2.3:44604087-44604106 | None:intergenic | 20.0% |
| !! | TGATAAAATTCTTGACATAA+AGG | + | chr2.3:44603506-44603525 | MS.gene062041:CDS | 20.0% |
| !! | TTAAGTGATAACTTATTCAT+TGG | + | chr2.3:44604536-44604555 | MS.gene062041:intron | 20.0% |
| !! | TTTGTTCAAGTGAATAAAAA+GGG | - | chr2.3:44604000-44604019 | None:intergenic | 20.0% |
| !!! | TTTTGTTCAAGTGAATAAAA+AGG | - | chr2.3:44604001-44604020 | None:intergenic | 20.0% |
| ! | AGATGATACAAAAATGAACA+AGG | - | chr2.3:44604033-44604052 | None:intergenic | 25.0% |
| ! | TTGTTAGTTATACCCTTATA+TGG | - | chr2.3:44604260-44604279 | None:intergenic | 25.0% |
| !!! | GTTTTTAATTCCATGAACAT+CGG | - | chr2.3:44604503-44604522 | None:intergenic | 25.0% |
| !!! | TGTTGTTTCTGAAAAAGATA+TGG | - | chr2.3:44604781-44604800 | None:intergenic | 25.0% |
| ACACGTAGTTTATTCACATT+GGG | + | chr2.3:44603957-44603976 | MS.gene062041:intron | 30.0% | |
| AGATATGAACAAGTAAATGC+AGG | - | chr2.3:44604693-44604712 | None:intergenic | 30.0% | |
| AGGTTGTACATACAATCTAA+AGG | - | chr2.3:44604124-44604143 | None:intergenic | 30.0% | |
| CAAACTAGTACGTTATTCAT+CGG | + | chr2.3:44604935-44604954 | MS.gene062041:intron | 30.0% | |
| GAAGCAATCAGAATTATTTC+AGG | - | chr2.3:44603766-44603785 | None:intergenic | 30.0% | |
| TAAAATTGTTGCATAAGGAC+AGG | - | chr2.3:44604144-44604163 | None:intergenic | 30.0% | |
| TACACGTAGTTTATTCACAT+TGG | + | chr2.3:44603956-44603975 | MS.gene062041:intron | 30.0% | |
| ! | TACTATCCTGTTAATTTTCC+AGG | + | chr2.3:44605010-44605029 | MS.gene062041:intron | 30.0% |
| ! | TAGTATGTTAGCTCTTATCA+TGG | - | chr2.3:44604995-44605014 | None:intergenic | 30.0% |
| !! | ATTCCTCTAAAGGATCATTT+TGG | + | chr2.3:44604730-44604749 | MS.gene062041:intron | 30.0% |
| !! | TTGTTTTATCTTGGCAACTT+TGG | - | chr2.3:44604342-44604361 | None:intergenic | 30.0% |
| !!! | AAACACACTCTAACTTGTTT+TGG | + | chr2.3:44603929-44603948 | MS.gene062041:intron | 30.0% |
| AACAAGTAACAAGCCACTAA+AGG | + | chr2.3:44604275-44604294 | MS.gene062041:intron | 35.0% | |
| AACACAGATAGAATCAAAGC+TGG | - | chr2.3:44603713-44603732 | None:intergenic | 35.0% | |
| AAGATATGGCAGTACACTAA+TGG | - | chr2.3:44604767-44604786 | None:intergenic | 35.0% | |
| AAGTGATTTCTATCAGACGA+GGG | + | chr2.3:44604869-44604888 | MS.gene062041:CDS | 35.0% | |
| ACCCTTATATGGTAGAAACA+GGG | - | chr2.3:44604249-44604268 | None:intergenic | 35.0% | |
| ACGTAGTTTATTCACATTGG+GGG | + | chr2.3:44603959-44603978 | MS.gene062041:intron | 35.0% | |
| ATTCATGAAGGTCTTCTAGA+TGG | + | chr2.3:44604578-44604597 | MS.gene062041:intron | 35.0% | |
| CACGTAGTTTATTCACATTG+GGG | + | chr2.3:44603958-44603977 | MS.gene062041:intron | 35.0% | |
| CATCCAAAATGATCCTTTAG+AGG | - | chr2.3:44604736-44604755 | None:intergenic | 35.0% | |
| CATTATCATCTGGAAGCAAA+AGG | - | chr2.3:44604821-44604840 | None:intergenic | 35.0% | |
| CGATGAATAACGTACTAGTT+TGG | - | chr2.3:44604937-44604956 | None:intergenic | 35.0% | |
| TACCCTTATATGGTAGAAAC+AGG | - | chr2.3:44604250-44604269 | None:intergenic | 35.0% | |
| TCCTCAAACTCATTATCATC+TGG | - | chr2.3:44604831-44604850 | None:intergenic | 35.0% | |
| TGACAACATCATTCCTCTAA+AGG | + | chr2.3:44604720-44604739 | MS.gene062041:intron | 35.0% | |
| ! | AGATGATAATGAGTTTGAGG+AGG | + | chr2.3:44604830-44604849 | MS.gene062041:intron | 35.0% |
| ! | TCCAGATGATAATGAGTTTG+AGG | + | chr2.3:44604827-44604846 | MS.gene062041:intron | 35.0% |
| ! | TGAATAACGTACTAGTTTGG+TGG | - | chr2.3:44604934-44604953 | None:intergenic | 35.0% |
| ! | TTTTATCAGATGCCTTGCAT+CGG | - | chr2.3:44603494-44603513 | None:intergenic | 35.0% |
| !! | TCCCTGTTTCTACCATATAA+GGG | + | chr2.3:44604245-44604264 | MS.gene062041:intron | 35.0% |
| !! | TTCCCTGTTTCTACCATATA+AGG | + | chr2.3:44604244-44604263 | MS.gene062041:intron | 35.0% |
| AACATACTTCATCCGATGCA+AGG | + | chr2.3:44603479-44603498 | MS.gene062041:CDS | 40.0% | |
| ACCTTGAGGAAAGGTAAACT+TGG | + | chr2.3:44603868-44603887 | MS.gene062041:intron | 40.0% | |
| ATGGCATTACTGTGAGGTTA+GGG | + | chr2.3:44604313-44604332 | MS.gene062041:intron | 40.0% | |
| CAAGTGATTTCTATCAGACG+AGG | + | chr2.3:44604868-44604887 | MS.gene062041:CDS | 40.0% | |
| CCTTGAGGAAAGGTAAACTT+GGG | + | chr2.3:44603869-44603888 | MS.gene062041:intron | 40.0% | |
| CGTAGTTTATTCACATTGGG+GGG | + | chr2.3:44603960-44603979 | MS.gene062041:intron | 40.0% | |
| CTCGTCTGATAGAAATCACT+TGG | - | chr2.3:44604870-44604889 | None:intergenic | 40.0% | |
| CTTGAGGAAAGGTAAACTTG+GGG | + | chr2.3:44603870-44603889 | MS.gene062041:intron | 40.0% | |
| TAAGACTCTGGCTGATCAAA+AGG | + | chr2.3:44603611-44603630 | MS.gene062041:CDS | 40.0% | |
| TATGGCATTACTGTGAGGTT+AGG | + | chr2.3:44604312-44604331 | MS.gene062041:intron | 40.0% | |
| TATTCACATTGGGGGGATAT+TGG | + | chr2.3:44603967-44603986 | MS.gene062041:intron | 40.0% | |
| ! | TGGCTTTACCAATTACCAAC+TGG | - | chr2.3:44604907-44604926 | None:intergenic | 40.0% |
| !! | AAGCCACTAAAGGTATTGTG+TGG | + | chr2.3:44604285-44604304 | MS.gene062041:intron | 40.0% |
| !! | AAGGTATTGTGTGGCTGTTA+TGG | + | chr2.3:44604294-44604313 | MS.gene062041:intron | 40.0% |
| !! | ATTAGACGACTCTAAGACTC+TGG | + | chr2.3:44603599-44603618 | MS.gene062041:CDS | 40.0% |
| ACAAACCTGCTAAGGACCAA+CGG | + | chr2.3:44603550-44603569 | MS.gene062041:CDS | 45.0% | |
| CAGCCACACAATACCTTTAG+TGG | - | chr2.3:44604291-44604310 | None:intergenic | 45.0% | |
| CCCAAGTTTACCTTTCCTCA+AGG | - | chr2.3:44603872-44603891 | None:intergenic | 45.0% | |
| GCTGTTATGGCATTACTGTG+AGG | + | chr2.3:44604307-44604326 | MS.gene062041:intron | 45.0% | |
| GGTAGAAACAGGGAAGTCAA+TGG | - | chr2.3:44604239-44604258 | None:intergenic | 45.0% | |
| TACCAGGTAGAATCAACCGT+TGG | - | chr2.3:44603569-44603588 | None:intergenic | 45.0% | |
| TCTAATACTTCCCCTGTACC+AGG | - | chr2.3:44603585-44603604 | None:intergenic | 45.0% | |
| TTTGTTCAGCTGCAGTTGTC+AGG | + | chr2.3:44604465-44604484 | MS.gene062041:intron | 45.0% | |
| ! | CGTACTAGTTTGGTGGAGTA+TGG | - | chr2.3:44604927-44604946 | None:intergenic | 45.0% |
| ! | GCTTGTTGACAAACCTGCTA+AGG | + | chr2.3:44603542-44603561 | MS.gene062041:CDS | 45.0% |
| ! | GGTTGATTCTACCTGGTACA+GGG | + | chr2.3:44603571-44603590 | MS.gene062041:CDS | 45.0% |
| ! | GTTGATTCTACCTGGTACAG+GGG | + | chr2.3:44603572-44603591 | MS.gene062041:CDS | 45.0% |
| !! | ATGGATTGGACCGATGTTCA+TGG | + | chr2.3:44604490-44604509 | MS.gene062041:intron | 45.0% |
| !! | AAATTTATGAATTTATATTA+GGG | + | chr2.3:44603901-44603920 | MS.gene062041:intron | 5.0% |
| !!! | ATTTTAAATTTGTTTAATAT+TGG | + | chr2.3:44604158-44604177 | MS.gene062041:intron | 5.0% |
| !!! | TTTTAAATTTGTTTAATATT+GGG | + | chr2.3:44604159-44604178 | MS.gene062041:intron | 5.0% |
| ATCAACCGTTGGTCCTTAGC+AGG | - | chr2.3:44603558-44603577 | None:intergenic | 50.0% | |
| ! | CGGTTGATTCTACCTGGTAC+AGG | + | chr2.3:44603570-44603589 | MS.gene062041:CDS | 50.0% |
| ! | CTGTTGTTGCACTGACCTTG+AGG | + | chr2.3:44603854-44603873 | MS.gene062041:CDS | 50.0% |
| ! | GACCAACGGTTGATTCTACC+TGG | + | chr2.3:44603564-44603583 | MS.gene062041:CDS | 50.0% |
| ! | GTTGCACTGACCTTGAGGAA+AGG | + | chr2.3:44603859-44603878 | MS.gene062041:CDS | 50.0% |
| !! | AGAGGGTGCCAGTTGGTAAT+TGG | + | chr2.3:44604896-44604915 | MS.gene062041:intron | 50.0% |
| !! | GCAGTTGTCAGGTGATGGAT+TGG | + | chr2.3:44604476-44604495 | MS.gene062041:intron | 50.0% |
| CAGCTGCAGTTGTCAGGTGA+TGG | + | chr2.3:44604471-44604490 | MS.gene062041:intron | 55.0% | |
| TATCAGACGAGGGACGCAGA+GGG | + | chr2.3:44604879-44604898 | MS.gene062041:CDS | 55.0% | |
| CTATCAGACGAGGGACGCAG+AGG | + | chr2.3:44604878-44604897 | MS.gene062041:CDS | 60.0% | |
| !! | GGGACGCAGAGGGTGCCAGT+TGG | + | chr2.3:44604889-44604908 | MS.gene062041:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 44603450 | 44605035 | 44603450 | ID=MS.gene062041 |
| chr2.3 | mRNA | 44603450 | 44605035 | 44603450 | ID=MS.gene062041.t1;Parent=MS.gene062041 |
| chr2.3 | exon | 44603450 | 44603632 | 44603450 | ID=MS.gene062041.t1.exon1;Parent=MS.gene062041.t1 |
| chr2.3 | CDS | 44603450 | 44603632 | 44603450 | ID=cds.MS.gene062041.t1;Parent=MS.gene062041.t1 |
| chr2.3 | exon | 44603841 | 44603880 | 44603841 | ID=MS.gene062041.t1.exon2;Parent=MS.gene062041.t1 |
| chr2.3 | CDS | 44603841 | 44603880 | 44603841 | ID=cds.MS.gene062041.t1;Parent=MS.gene062041.t1 |
| chr2.3 | exon | 44604832 | 44604910 | 44604832 | ID=MS.gene062041.t1.exon3;Parent=MS.gene062041.t1 |
| chr2.3 | CDS | 44604832 | 44604910 | 44604832 | ID=cds.MS.gene062041.t1;Parent=MS.gene062041.t1 |
| chr2.3 | exon | 44605032 | 44605035 | 44605032 | ID=MS.gene062041.t1.exon4;Parent=MS.gene062041.t1 |
| chr2.3 | CDS | 44605032 | 44605035 | 44605032 | ID=cds.MS.gene062041.t1;Parent=MS.gene062041.t1 |
| Gene Sequence |
| Protein sequence |
