Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062439.t1 | XP_003621878.2 | 92.3 | 350 | 27 | 0 | 1 | 350 | 1 | 350 | 2.00E-185 | 658.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062439.t1 | Q84KK5 | 55.0 | 353 | 151 | 3 | 3 | 348 | 4 | 355 | 4.9e-108 | 392.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062439.t1 | G7KWV7 | 92.3 | 350 | 27 | 0 | 1 | 350 | 1 | 350 | 1.4e-185 | 658.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050883 | MS.gene062439 | 0.808767 | 2.63E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene062439 | 0.807161 | 5.79E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene062439 | 0.813517 | 2.46E-51 | -1.69E-46 |
| MS.gene054056 | MS.gene062439 | 0.822313 | 2.53E-53 | -1.69E-46 |
| MS.gene055942 | MS.gene062439 | 0.807092 | 5.98E-50 | -1.69E-46 |
| MS.gene056311 | MS.gene062439 | 0.803107 | 4.08E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene062439 | 0.828859 | 7.11E-55 | -1.69E-46 |
| MS.gene058109 | MS.gene062439 | 0.805657 | 1.20E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene062439 | 0.816039 | 6.79E-52 | -1.69E-46 |
| MS.gene058494 | MS.gene062439 | 0.802717 | 4.91E-49 | -1.69E-46 |
| MS.gene058628 | MS.gene062439 | 0.818183 | 2.24E-52 | -1.69E-46 |
| MS.gene058629 | MS.gene062439 | 0.8157 | 8.08E-52 | -1.69E-46 |
| MS.gene058630 | MS.gene062439 | 0.812533 | 4.04E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062439.t1 | MTR_7g024500 | 92.286 | 350 | 27 | 0 | 1 | 350 | 1 | 350 | 0.0 | 675 |
| MS.gene062439.t1 | MTR_7g024520 | 86.571 | 350 | 47 | 0 | 1 | 350 | 1 | 350 | 0.0 | 632 |
| MS.gene062439.t1 | MTR_4g088345 | 64.124 | 354 | 121 | 2 | 1 | 348 | 1 | 354 | 1.65e-170 | 479 |
| MS.gene062439.t1 | MTR_4g088340 | 64.653 | 331 | 112 | 1 | 23 | 348 | 1 | 331 | 1.04e-158 | 448 |
| MS.gene062439.t1 | MTR_7g014570 | 55.714 | 350 | 148 | 3 | 3 | 348 | 4 | 350 | 1.38e-138 | 397 |
| MS.gene062439.t1 | MTR_1g076940 | 54.494 | 356 | 150 | 5 | 3 | 347 | 4 | 358 | 1.76e-137 | 395 |
| MS.gene062439.t1 | MTR_7g014510 | 52.825 | 354 | 159 | 3 | 2 | 348 | 3 | 355 | 8.08e-132 | 380 |
| MS.gene062439.t1 | MTR_5g074680 | 46.648 | 358 | 181 | 5 | 1 | 348 | 1 | 358 | 1.35e-112 | 332 |
| MS.gene062439.t1 | MTR_4g088200 | 46.893 | 354 | 178 | 4 | 5 | 348 | 6 | 359 | 1.57e-112 | 332 |
| MS.gene062439.t1 | MTR_4g033150 | 48.324 | 358 | 173 | 4 | 3 | 348 | 4 | 361 | 8.71e-111 | 327 |
| MS.gene062439.t1 | MTR_5g074600 | 46.499 | 357 | 182 | 6 | 1 | 348 | 1 | 357 | 1.83e-110 | 326 |
| MS.gene062439.t1 | MTR_4g088190 | 48.468 | 359 | 172 | 4 | 3 | 348 | 4 | 362 | 7.11e-110 | 325 |
| MS.gene062439.t1 | MTR_4g033015 | 47.765 | 358 | 175 | 4 | 3 | 348 | 4 | 361 | 1.02e-109 | 325 |
| MS.gene062439.t1 | MTR_5g074580 | 45.869 | 351 | 182 | 5 | 6 | 348 | 8 | 358 | 2.22e-108 | 321 |
| MS.gene062439.t1 | MTR_4g033200 | 47.207 | 358 | 177 | 4 | 3 | 348 | 4 | 361 | 3.42e-108 | 321 |
| MS.gene062439.t1 | MTR_4g079160 | 44.915 | 354 | 188 | 3 | 1 | 348 | 1 | 353 | 1.35e-107 | 319 |
| MS.gene062439.t1 | MTR_4g033170 | 48.718 | 351 | 172 | 4 | 5 | 348 | 6 | 355 | 1.94e-105 | 313 |
| MS.gene062439.t1 | MTR_4g033085 | 48.148 | 351 | 174 | 4 | 5 | 348 | 6 | 355 | 2.31e-98 | 295 |
| MS.gene062439.t1 | MTR_5g074610 | 41.379 | 348 | 171 | 4 | 7 | 348 | 4 | 324 | 1.05e-94 | 285 |
| MS.gene062439.t1 | MTR_5g074540 | 42.120 | 349 | 180 | 5 | 6 | 348 | 7 | 339 | 1.51e-94 | 285 |
| MS.gene062439.t1 | MTR_1g076930 | 50.211 | 237 | 109 | 4 | 23 | 250 | 1 | 237 | 6.58e-76 | 234 |
| MS.gene062439.t1 | MTR_3g083620 | 37.463 | 339 | 199 | 7 | 19 | 349 | 27 | 360 | 5.03e-73 | 231 |
| MS.gene062439.t1 | MTR_0794s0020 | 41.870 | 246 | 131 | 4 | 3 | 236 | 4 | 249 | 1.91e-63 | 202 |
| MS.gene062439.t1 | MTR_0017s0100 | 34.648 | 355 | 218 | 8 | 2 | 347 | 16 | 365 | 2.61e-57 | 190 |
| MS.gene062439.t1 | MTR_4g038440 | 33.918 | 342 | 214 | 7 | 15 | 347 | 27 | 365 | 4.98e-56 | 187 |
| MS.gene062439.t1 | MTR_0079s0070 | 34.639 | 332 | 205 | 7 | 25 | 347 | 3 | 331 | 2.32e-55 | 184 |
| MS.gene062439.t1 | MTR_3g092900 | 32.011 | 353 | 219 | 12 | 1 | 336 | 1 | 349 | 1.22e-44 | 157 |
| MS.gene062439.t1 | MTR_5g098170 | 33.022 | 321 | 199 | 8 | 29 | 336 | 32 | 349 | 3.65e-43 | 153 |
| MS.gene062439.t1 | MTR_7g012090 | 28.909 | 339 | 216 | 8 | 29 | 345 | 38 | 373 | 9.24e-41 | 147 |
| MS.gene062439.t1 | MTR_7g012040 | 27.327 | 333 | 223 | 7 | 29 | 345 | 37 | 366 | 1.53e-40 | 146 |
| MS.gene062439.t1 | MTR_7g012070 | 28.319 | 339 | 218 | 9 | 29 | 345 | 38 | 373 | 3.69e-40 | 145 |
| MS.gene062439.t1 | MTR_1g096310 | 30.031 | 323 | 208 | 7 | 29 | 336 | 38 | 357 | 8.59e-40 | 144 |
| MS.gene062439.t1 | MTR_1g036490 | 29.023 | 348 | 205 | 7 | 17 | 336 | 21 | 354 | 1.40e-39 | 144 |
| MS.gene062439.t1 | MTR_1g036510 | 29.499 | 339 | 199 | 7 | 24 | 336 | 44 | 368 | 2.69e-39 | 143 |
| MS.gene062439.t1 | MTR_7g011900 | 27.493 | 371 | 240 | 9 | 1 | 345 | 1 | 368 | 4.99e-39 | 142 |
| MS.gene062439.t1 | MTR_1g036460 | 29.023 | 348 | 205 | 7 | 17 | 336 | 21 | 354 | 1.85e-38 | 140 |
| MS.gene062439.t1 | MTR_8g068870 | 27.089 | 347 | 231 | 8 | 3 | 331 | 2 | 344 | 8.54e-38 | 139 |
| MS.gene062439.t1 | MTR_7g118300 | 29.721 | 323 | 211 | 8 | 25 | 335 | 42 | 360 | 1.36e-37 | 139 |
| MS.gene062439.t1 | MTR_0591s0010 | 27.089 | 347 | 232 | 9 | 3 | 332 | 17 | 359 | 2.09e-37 | 138 |
| MS.gene062439.t1 | MTR_7g012020 | 27.532 | 316 | 213 | 6 | 29 | 331 | 37 | 349 | 3.70e-37 | 137 |
| MS.gene062439.t1 | MTR_7g011990 | 28.571 | 273 | 175 | 8 | 73 | 331 | 81 | 347 | 5.33e-37 | 137 |
| MS.gene062439.t1 | MTR_8g068750 | 27.103 | 321 | 217 | 7 | 25 | 331 | 41 | 358 | 1.47e-36 | 136 |
| MS.gene062439.t1 | MTR_7g118320 | 27.299 | 348 | 221 | 10 | 4 | 331 | 8 | 343 | 5.55e-35 | 131 |
| MS.gene062439.t1 | MTR_8g024160 | 30.293 | 307 | 187 | 10 | 44 | 335 | 19 | 313 | 6.77e-35 | 130 |
| MS.gene062439.t1 | MTR_2g055940 | 27.019 | 322 | 214 | 9 | 29 | 335 | 32 | 347 | 2.11e-34 | 130 |
| MS.gene062439.t1 | MTR_3g021430 | 30.469 | 256 | 168 | 5 | 95 | 345 | 100 | 350 | 1.65e-32 | 124 |
| MS.gene062439.t1 | MTR_8g068690 | 26.708 | 322 | 217 | 7 | 25 | 331 | 40 | 357 | 4.15e-32 | 124 |
| MS.gene062439.t1 | MTR_3g021440 | 26.183 | 317 | 218 | 7 | 32 | 336 | 43 | 355 | 2.33e-31 | 122 |
| MS.gene062439.t1 | MTR_6g078850 | 26.685 | 356 | 221 | 11 | 25 | 349 | 45 | 391 | 3.16e-31 | 123 |
| MS.gene062439.t1 | MTR_3g096050 | 28.783 | 337 | 214 | 12 | 29 | 348 | 29 | 356 | 1.54e-30 | 119 |
| MS.gene062439.t1 | MTR_8g068570 | 28.188 | 298 | 175 | 8 | 3 | 288 | 17 | 287 | 5.05e-30 | 117 |
| MS.gene062439.t1 | MTR_2g072790 | 28.070 | 342 | 211 | 11 | 29 | 349 | 26 | 353 | 8.26e-30 | 117 |
| MS.gene062439.t1 | MTR_4g038440 | 30.738 | 244 | 161 | 4 | 15 | 250 | 27 | 270 | 1.08e-27 | 109 |
| MS.gene062439.t1 | MTR_8g068730 | 27.129 | 317 | 190 | 10 | 25 | 331 | 41 | 326 | 1.41e-27 | 111 |
| MS.gene062439.t1 | MTR_3g096070 | 28.135 | 327 | 206 | 11 | 29 | 348 | 29 | 333 | 1.44e-27 | 110 |
| MS.gene062439.t1 | MTR_1g050220 | 36.054 | 147 | 81 | 2 | 185 | 331 | 151 | 284 | 4.33e-24 | 100 |
| MS.gene062439.t1 | MTR_7g012020 | 26.778 | 239 | 162 | 5 | 29 | 254 | 37 | 275 | 5.82e-24 | 100 |
| MS.gene062439.t1 | MTR_1g036510 | 31.613 | 155 | 95 | 1 | 96 | 250 | 142 | 285 | 4.04e-20 | 89.7 |
| MS.gene062439.t1 | MTR_8g068740 | 27.309 | 249 | 144 | 8 | 25 | 264 | 41 | 261 | 6.14e-19 | 85.9 |
| MS.gene062439.t1 | MTR_8g068600 | 25.000 | 228 | 158 | 5 | 37 | 251 | 27 | 254 | 7.60e-19 | 85.9 |
| MS.gene062439.t1 | MTR_8g068620 | 39.175 | 97 | 59 | 0 | 157 | 253 | 17 | 113 | 1.32e-17 | 78.2 |
| MS.gene062439.t1 | MTR_8g068690 | 25.301 | 249 | 159 | 7 | 25 | 253 | 40 | 281 | 1.52e-17 | 82.0 |
| MS.gene062439.t1 | MTR_7g012020 | 25.121 | 207 | 142 | 5 | 29 | 222 | 37 | 243 | 2.22e-15 | 75.5 |
| MS.gene062439.t1 | MTR_3g096050 | 28.333 | 240 | 151 | 10 | 29 | 253 | 29 | 262 | 8.27e-14 | 70.9 |
| MS.gene062439.t1 | MTR_4g025570 | 45.679 | 81 | 40 | 3 | 252 | 331 | 122 | 199 | 1.45e-13 | 69.3 |
| MS.gene062439.t1 | MTR_4g025570 | 35.922 | 103 | 53 | 4 | 4 | 101 | 13 | 107 | 9.84e-11 | 61.2 |
| MS.gene062439.t1 | MTR_1g036510 | 28.346 | 127 | 80 | 1 | 96 | 222 | 142 | 257 | 1.88e-12 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062439.t1 | AT4G35150 | 34.262 | 359 | 184 | 10 | 1 | 346 | 1 | 320 | 7.02e-58 | 191 |
| MS.gene062439.t1 | AT4G35160 | 36.592 | 358 | 196 | 13 | 14 | 346 | 26 | 377 | 6.89e-54 | 182 |
| MS.gene062439.t1 | AT5G54160 | 32.143 | 364 | 226 | 12 | 1 | 348 | 1 | 359 | 4.92e-48 | 166 |
| MS.gene062439.t1 | AT1G51990 | 32.857 | 280 | 162 | 8 | 85 | 348 | 91 | 360 | 1.20e-39 | 144 |
| MS.gene062439.t1 | AT1G51990 | 32.857 | 280 | 162 | 8 | 85 | 348 | 91 | 360 | 1.47e-39 | 144 |
| MS.gene062439.t1 | AT1G33030 | 29.430 | 316 | 206 | 8 | 29 | 331 | 20 | 331 | 2.69e-34 | 129 |
| MS.gene062439.t1 | AT3G53140 | 29.942 | 344 | 205 | 11 | 29 | 348 | 26 | 357 | 3.40e-34 | 129 |
| MS.gene062439.t1 | AT1G77520 | 25.581 | 344 | 225 | 9 | 29 | 348 | 43 | 379 | 1.69e-30 | 119 |
| MS.gene062439.t1 | AT1G63140 | 28.125 | 352 | 218 | 11 | 23 | 348 | 57 | 399 | 2.04e-28 | 114 |
| MS.gene062439.t1 | AT1G63140 | 28.125 | 352 | 218 | 11 | 23 | 348 | 37 | 379 | 2.32e-28 | 114 |
| MS.gene062439.t1 | AT5G53810 | 30.347 | 346 | 207 | 12 | 29 | 348 | 39 | 376 | 2.83e-28 | 113 |
| MS.gene062439.t1 | AT1G62900 | 33.171 | 205 | 130 | 5 | 147 | 348 | 3 | 203 | 1.40e-26 | 105 |
| MS.gene062439.t1 | AT1G77530 | 27.168 | 346 | 217 | 10 | 29 | 348 | 43 | 379 | 5.48e-24 | 101 |
| MS.gene062439.t1 | AT5G37170 | 32.597 | 181 | 110 | 6 | 157 | 331 | 41 | 215 | 4.82e-22 | 93.6 |
| MS.gene062439.t1 | AT5G37170 | 32.597 | 181 | 110 | 6 | 157 | 331 | 141 | 315 | 6.22e-22 | 95.5 |
| MS.gene062439.t1 | AT1G21130 | 26.444 | 329 | 203 | 12 | 29 | 331 | 39 | 354 | 1.00e-20 | 92.4 |
| MS.gene062439.t1 | AT5G37170 | 32.749 | 171 | 104 | 6 | 167 | 331 | 2 | 167 | 5.76e-20 | 86.7 |
| MS.gene062439.t1 | AT1G21100 | 27.108 | 332 | 197 | 14 | 29 | 331 | 39 | 354 | 9.16e-20 | 89.7 |
| MS.gene062439.t1 | AT1G76790 | 28.182 | 330 | 198 | 13 | 29 | 331 | 29 | 346 | 9.27e-20 | 89.7 |
| MS.gene062439.t1 | AT1G21110 | 25.305 | 328 | 208 | 11 | 29 | 331 | 39 | 354 | 9.60e-19 | 86.7 |
| MS.gene062439.t1 | AT1G21120 | 25.000 | 328 | 212 | 10 | 29 | 334 | 39 | 354 | 4.86e-18 | 84.7 |
| MS.gene062439.t1 | AT1G21120 | 25.000 | 328 | 212 | 10 | 29 | 334 | 87 | 402 | 5.69e-18 | 85.1 |
| MS.gene062439.t1 | AT1G33030 | 26.496 | 234 | 159 | 6 | 29 | 250 | 20 | 252 | 1.46e-14 | 73.2 |
| MS.gene062439.t1 | AT1G33030 | 26.496 | 234 | 159 | 6 | 29 | 250 | 20 | 252 | 2.64e-14 | 72.8 |
| MS.gene062439.t1 | AT1G63140 | 26.531 | 245 | 152 | 6 | 29 | 250 | 63 | 302 | 3.68e-11 | 63.9 |
Find 54 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTTCATGCAACAAAATT+TGG | 0.173410 | 7.3:+75495337 | MS.gene062439:CDS |
| TACAAAATATTTCCTATATT+TGG | 0.180034 | 7.3:+75496372 | MS.gene062439:CDS |
| TAATAGGTTCGCCATGATTT+TGG | 0.194358 | 7.3:-75495025 | None:intergenic |
| TCACGGTGTCATGCGTTTAT+TGG | 0.277803 | 7.3:+75495103 | MS.gene062439:CDS |
| ACAAGCTCAGGAAGAGTAAT+AGG | 0.291026 | 7.3:-75495041 | None:intergenic |
| CAATCTTTGAGACTACATTA+TGG | 0.292380 | 7.3:+75495315 | MS.gene062439:CDS |
| GTTAATCCTTCTACCACATT+TGG | 0.348422 | 7.3:-75495545 | None:intergenic |
| AATCTTTGAGACTACATTAT+GGG | 0.349412 | 7.3:+75495316 | MS.gene062439:CDS |
| TGATCATTGTTTAACTTCAA+TGG | 0.378501 | 7.3:+75495217 | MS.gene062439:CDS |
| AATATTATCAATTGAGCAAA+TGG | 0.394667 | 7.3:+75495273 | MS.gene062439:CDS |
| TTTAACTTCAATGGTTAAGA+TGG | 0.403378 | 7.3:+75495226 | MS.gene062439:CDS |
| TTAACTTCTTTGGTTGATGT+TGG | 0.411610 | 7.3:+75495449 | MS.gene062439:CDS |
| ACTTCTTTGGTTGATGTTGG+AGG | 0.420904 | 7.3:+75495452 | MS.gene062439:CDS |
| GACCTTCCAAATGTGGTAGA+AGG | 0.429597 | 7.3:+75495539 | MS.gene062439:CDS |
| ATTGTAAAGAAGCTGCTTCA+AGG | 0.431595 | 7.3:+75496172 | MS.gene062439:CDS |
| AGTGACCTAAAACCAAATAT+AGG | 0.433185 | 7.3:-75496384 | None:intergenic |
| TACTCTTCCTGAGCTTGTCT+CGG | 0.435364 | 7.3:+75495046 | MS.gene062439:CDS |
| CATGACACCGTGAACACAAC+CGG | 0.452264 | 7.3:-75495093 | None:intergenic |
| AAATGTGGTAGAAGGATTAA+CGG | 0.461848 | 7.3:+75495547 | MS.gene062439:CDS |
| AGACATTGTGATAAATGAAA+AGG | 0.464272 | 7.3:+75496224 | MS.gene062439:CDS |
| ATCAAGTGGGCTGTTGAACT+TGG | 0.469328 | 7.3:+75494987 | MS.gene062439:CDS |
| AATGTGGTAGAAGGATTAAC+GGG | 0.469980 | 7.3:+75495548 | MS.gene062439:CDS |
| GTTGATGTTGGAGGTGGAAC+CGG | 0.470193 | 7.3:+75495461 | MS.gene062439:CDS |
| TAATATTTATCAACCATGGT+AGG | 0.470597 | 7.3:-75495257 | None:intergenic |
| GCAAAAGCCGGTTGTGTTCA+CGG | 0.474267 | 7.3:+75495086 | MS.gene062439:CDS |
| AAGAAAATCATAACAGAAGC+AGG | 0.478707 | 7.3:+75496339 | MS.gene062439:CDS |
| GTAAAATTGCATCAGCTTGA+GGG | 0.481886 | 7.3:-75495616 | None:intergenic |
| AGTAAAATTGCATCAGCTTG+AGG | 0.494453 | 7.3:-75495617 | None:intergenic |
| TTCACAAGAAGCTTTGATGT+TGG | 0.499286 | 7.3:-75495188 | None:intergenic |
| CTGCTTTGATATAACTATGA+TGG | 0.505550 | 7.3:+75496278 | MS.gene062439:CDS |
| CACGGTTAAAATTATTTGTG+AGG | 0.517597 | 7.3:+75495487 | MS.gene062439:CDS |
| CGAAGAGCCGAGACAAGCTC+AGG | 0.517956 | 7.3:-75495053 | None:intergenic |
| AATATTTATCAACCATGGTA+GGG | 0.522593 | 7.3:-75495256 | None:intergenic |
| CCAGATATAATCCAAAATCA+TGG | 0.539133 | 7.3:+75495014 | MS.gene062439:CDS |
| ACATCAAAGCTTCTTGTGAA+AGG | 0.547089 | 7.3:+75495191 | MS.gene062439:CDS |
| TGAGACCCGCGTCGATCAAG+TGG | 0.547754 | 7.3:+75494973 | MS.gene062439:CDS |
| CGAATTCCAGAAGCAAAAGC+CGG | 0.553157 | 7.3:+75495074 | MS.gene062439:CDS |
| TCTTTGGTTGATGTTGGAGG+TGG | 0.553343 | 7.3:+75495455 | MS.gene062439:CDS |
| ATCCTTCTACCACATTTGGA+AGG | 0.561989 | 7.3:-75495541 | None:intergenic |
| AATTGCATCAGCTTGAGGGA+TGG | 0.574477 | 7.3:-75495612 | None:intergenic |
| TTGATAATATTTATCAACCA+TGG | 0.592376 | 7.3:-75495261 | None:intergenic |
| GAAGCTATTCAATGATGCTA+TGG | 0.595485 | 7.3:+75495367 | MS.gene062439:CDS |
| ATTGAGCAAATGGACATCTA+CGG | 0.596800 | 7.3:+75495283 | MS.gene062439:CDS |
| AGTGTTCGACCTTCCAAATG+TGG | 0.610487 | 7.3:+75495532 | MS.gene062439:CDS |
| AGAGTGATTCTAATATGATG+AGG | 0.631037 | 7.3:+75495390 | MS.gene062439:CDS |
| ACAGCCCACTTGATCGACGC+GGG | 0.642324 | 7.3:-75494978 | None:intergenic |
| GCTTCAAGGCAAAACAAAGG+AGG | 0.643659 | 7.3:+75496186 | MS.gene062439:CDS |
| TGGAGGTGGAACCGGAAACA+CGG | 0.653524 | 7.3:+75495469 | MS.gene062439:CDS |
| GATGGTAACAAACCCTACCA+TGG | 0.661901 | 7.3:+75495244 | MS.gene062439:CDS |
| GCTGCTTCAAGGCAAAACAA+AGG | 0.669930 | 7.3:+75496183 | MS.gene062439:CDS |
| GAGACCCGCGTCGATCAAGT+GGG | 0.673235 | 7.3:+75494974 | MS.gene062439:CDS |
| TGTCAAGATAGATGACAACA+TGG | 0.674692 | 7.3:+75495148 | MS.gene062439:CDS |
| GAGCAAATGGACATCTACGG+AGG | 0.717467 | 7.3:+75495286 | MS.gene062439:CDS |
| AACAGCCCACTTGATCGACG+CGG | 0.727407 | 7.3:-75494979 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAACATAGATAAGA+AGG | + | chr7.3:75495868-75495887 | MS.gene062439:intron | 15.0% |
| !! | AAAAAAAACATAGATAAGAA+GGG | + | chr7.3:75495869-75495888 | MS.gene062439:intron | 15.0% |
| !! | AAGTAAAACATAGATAATAA+GGG | + | chr7.3:75495725-75495744 | MS.gene062439:intron | 15.0% |
| !! | TACAAAATATTTCCTATATT+TGG | + | chr7.3:75496372-75496391 | MS.gene062439:CDS | 15.0% |
| !! | TTAAGCTAATTAATAATGTA+TGG | - | chr7.3:75496083-75496102 | None:intergenic | 15.0% |
| !! | AAAAAACTATGATACTATCT+TGG | - | chr7.3:75495942-75495961 | None:intergenic | 20.0% |
| !! | AATATTATCAATTGAGCAAA+TGG | + | chr7.3:75495273-75495292 | MS.gene062439:CDS | 20.0% |
| !! | GAAGTAAAACATAGATAATA+AGG | + | chr7.3:75495724-75495743 | MS.gene062439:intron | 20.0% |
| !!! | AATAACTATCTAAGTTTTGT+CGG | + | chr7.3:75495572-75495591 | MS.gene062439:CDS | 20.0% |
| !!! | TAACAATACTTTGTTATGTA+TGG | - | chr7.3:75496058-75496077 | None:intergenic | 20.0% |
| !!! | TATTATCTATGTTTTACTTC+AGG | - | chr7.3:75495724-75495743 | None:intergenic | 20.0% |
| !!! | TATTTCCTATATTTGGTTTT+AGG | + | chr7.3:75496379-75496398 | MS.gene062439:CDS | 20.0% |
| !!! | TTGATAATATTTATCAACCA+TGG | - | chr7.3:75495264-75495283 | None:intergenic | 20.0% |
| ! | AAGGTACGTATTAATTCAAA+GGG | + | chr7.3:75495641-75495660 | MS.gene062439:intron | 25.0% |
| ! | AATATTTATCAACCATGGTA+GGG | - | chr7.3:75495259-75495278 | None:intergenic | 25.0% |
| ! | AGACATTGTGATAAATGAAA+AGG | + | chr7.3:75496224-75496243 | MS.gene062439:CDS | 25.0% |
| ! | TAATATTTATCAACCATGGT+AGG | - | chr7.3:75495260-75495279 | None:intergenic | 25.0% |
| ! | TTAATTCAAAGGGAAATGAA+TGG | + | chr7.3:75495651-75495670 | MS.gene062439:intron | 25.0% |
| !! | TGATCATTGTTTAACTTCAA+TGG | + | chr7.3:75495217-75495236 | MS.gene062439:CDS | 25.0% |
| !! | TTTAACTTCAATGGTTAAGA+TGG | + | chr7.3:75495226-75495245 | MS.gene062439:CDS | 25.0% |
| !!! | AACACTATACATTTTAACGT+AGG | - | chr7.3:75495518-75495537 | None:intergenic | 25.0% |
| !!! | AATAATTTTAACCGTGTTTC+CGG | - | chr7.3:75495483-75495502 | None:intergenic | 25.0% |
| !!! | AATCTTTGAGACTACATTAT+GGG | + | chr7.3:75495316-75495335 | MS.gene062439:CDS | 25.0% |
| !!! | TTCCTTTTCATTTTTGTAGT+GGG | + | chr7.3:75496104-75496123 | MS.gene062439:intron | 25.0% |
| !!! | TTTCCTTTTCATTTTTGTAG+TGG | + | chr7.3:75496103-75496122 | MS.gene062439:intron | 25.0% |
| !!! | TTTCTTTTTAAGTAGCCTAA+CGG | - | chr7.3:75495851-75495870 | None:intergenic | 25.0% |
| AAATGTGGTAGAAGGATTAA+CGG | + | chr7.3:75495547-75495566 | MS.gene062439:CDS | 30.0% | |
| AACCCACTACAAAAATGAAA+AGG | - | chr7.3:75496109-75496128 | None:intergenic | 30.0% | |
| AAGAAAATCATAACAGAAGC+AGG | + | chr7.3:75496339-75496358 | MS.gene062439:CDS | 30.0% | |
| AGAGTGATTCTAATATGATG+AGG | + | chr7.3:75495390-75495409 | MS.gene062439:CDS | 30.0% | |
| AGTGACCTAAAACCAAATAT+AGG | - | chr7.3:75496387-75496406 | None:intergenic | 30.0% | |
| ATAGATTCGCAATTAGGTAA+AGG | - | chr7.3:75495688-75495707 | None:intergenic | 30.0% | |
| CAAGGTACGTATTAATTCAA+AGG | + | chr7.3:75495640-75495659 | MS.gene062439:intron | 30.0% | |
| CACGGTTAAAATTATTTGTG+AGG | + | chr7.3:75495487-75495506 | MS.gene062439:CDS | 30.0% | |
| CCAGATATAATCCAAAATCA+TGG | + | chr7.3:75495014-75495033 | MS.gene062439:CDS | 30.0% | |
| CTAATTGCGAATCTATATGT+CGG | + | chr7.3:75495692-75495711 | MS.gene062439:intron | 30.0% | |
| CTGCTTTGATATAACTATGA+TGG | + | chr7.3:75496278-75496297 | MS.gene062439:CDS | 30.0% | |
| GAGAAAGAGATGAAAAAACA+TGG | + | chr7.3:75496316-75496335 | MS.gene062439:CDS | 30.0% | |
| GGATTTCATGCAACAAAATT+TGG | + | chr7.3:75495337-75495356 | MS.gene062439:CDS | 30.0% | |
| TTGTTCCTAAGTTAAGTTCA+CGG | - | chr7.3:75495754-75495773 | None:intergenic | 30.0% | |
| ! | CCATGATTTTGGATTATATC+TGG | - | chr7.3:75495017-75495036 | None:intergenic | 30.0% |
| ! | GATTGCAAATCAGTTTTTGA+GGG | + | chr7.3:75495425-75495444 | MS.gene062439:CDS | 30.0% |
| ! | TGATTGCAAATCAGTTTTTG+AGG | + | chr7.3:75495424-75495443 | MS.gene062439:CDS | 30.0% |
| ! | TTAACTTCTTTGGTTGATGT+TGG | + | chr7.3:75495449-75495468 | MS.gene062439:CDS | 30.0% |
| !! | CAATCTTTGAGACTACATTA+TGG | + | chr7.3:75495315-75495334 | MS.gene062439:CDS | 30.0% |
| !!! | ACCTTTTTAAGATGAATACG+TGG | - | chr7.3:75495818-75495837 | None:intergenic | 30.0% |
| !!! | CCTTTTTAAGATGAATACGT+GGG | - | chr7.3:75495817-75495836 | None:intergenic | 30.0% |
| AATGTGGTAGAAGGATTAAC+GGG | + | chr7.3:75495548-75495567 | MS.gene062439:CDS | 35.0% | |
| ACATCAAAGCTTCTTGTGAA+AGG | + | chr7.3:75495191-75495210 | MS.gene062439:CDS | 35.0% | |
| AGTAAAATTGCATCAGCTTG+AGG | - | chr7.3:75495620-75495639 | None:intergenic | 35.0% | |
| ATTGAGCAAATGGACATCTA+CGG | + | chr7.3:75495283-75495302 | MS.gene062439:CDS | 35.0% | |
| ATTGTAAAGAAGCTGCTTCA+AGG | + | chr7.3:75496172-75496191 | MS.gene062439:CDS | 35.0% | |
| CCCACGTATTCATCTTAAAA+AGG | + | chr7.3:75495814-75495833 | MS.gene062439:intron | 35.0% | |
| CGACATATAGATTCGCAATT+AGG | - | chr7.3:75495694-75495713 | None:intergenic | 35.0% | |
| GAAGCTATTCAATGATGCTA+TGG | + | chr7.3:75495367-75495386 | MS.gene062439:CDS | 35.0% | |
| GTAAAATTGCATCAGCTTGA+GGG | - | chr7.3:75495619-75495638 | None:intergenic | 35.0% | |
| GTTAATCCTTCTACCACATT+TGG | - | chr7.3:75495548-75495567 | None:intergenic | 35.0% | |
| TGCCTAATATATGCAGATGT+CGG | + | chr7.3:75495774-75495793 | MS.gene062439:intron | 35.0% | |
| TGTCAAGATAGATGACAACA+TGG | + | chr7.3:75495148-75495167 | MS.gene062439:CDS | 35.0% | |
| TGTTCCTAAGTTAAGTTCAC+GGG | - | chr7.3:75495753-75495772 | None:intergenic | 35.0% | |
| ! | AGCTGATGCAATTTTACTCA+AGG | + | chr7.3:75495622-75495641 | MS.gene062439:CDS | 35.0% |
| ! | TAATAGGTTCGCCATGATTT+TGG | - | chr7.3:75495028-75495047 | None:intergenic | 35.0% |
| ! | TTCACAAGAAGCTTTGATGT+TGG | - | chr7.3:75495191-75495210 | None:intergenic | 35.0% |
| !! | AACTATCTAAGTTTTGTCGG+CGG | + | chr7.3:75495575-75495594 | MS.gene062439:CDS | 35.0% |
| !! | TTTTGAGGGCTTAACTTCTT+TGG | + | chr7.3:75495439-75495458 | MS.gene062439:CDS | 35.0% |
| ACAAGCTCAGGAAGAGTAAT+AGG | - | chr7.3:75495044-75495063 | None:intergenic | 40.0% | |
| ATCCTTCTACCACATTTGGA+AGG | - | chr7.3:75495544-75495563 | None:intergenic | 40.0% | |
| CTCCGACATCTGCATATATT+AGG | - | chr7.3:75495779-75495798 | None:intergenic | 40.0% | |
| ! | ACTTCTTTGGTTGATGTTGG+AGG | + | chr7.3:75495452-75495471 | MS.gene062439:CDS | 40.0% |
| !! | AAGGTGAATTTCTAGCCGTT+AGG | + | chr7.3:75495833-75495852 | MS.gene062439:intron | 40.0% |
| !!! | TGTAGTGGGTTTTGCATAAC+TGG | + | chr7.3:75496118-75496137 | MS.gene062439:intron | 40.0% |
| AAGATGAATACGTGGGTGAC+CGG | - | chr7.3:75495810-75495829 | None:intergenic | 45.0% | |
| AATTGCATCAGCTTGAGGGA+TGG | - | chr7.3:75495615-75495634 | None:intergenic | 45.0% | |
| AGTGTTCGACCTTCCAAATG+TGG | + | chr7.3:75495532-75495551 | MS.gene062439:CDS | 45.0% | |
| CGAATTCCAGAAGCAAAAGC+CGG | + | chr7.3:75495074-75495093 | MS.gene062439:CDS | 45.0% | |
| GACCTTCCAAATGTGGTAGA+AGG | + | chr7.3:75495539-75495558 | MS.gene062439:CDS | 45.0% | |
| GATGGTAACAAACCCTACCA+TGG | + | chr7.3:75495244-75495263 | MS.gene062439:CDS | 45.0% | |
| GCTGCTTCAAGGCAAAACAA+AGG | + | chr7.3:75496183-75496202 | MS.gene062439:CDS | 45.0% | |
| GCTTCAAGGCAAAACAAAGG+AGG | + | chr7.3:75496186-75496205 | MS.gene062439:CDS | 45.0% | |
| GGTACCCGTGAACTTAACTT+AGG | + | chr7.3:75495746-75495765 | MS.gene062439:intron | 45.0% | |
| TACTCTTCCTGAGCTTGTCT+CGG | + | chr7.3:75495046-75495065 | MS.gene062439:CDS | 45.0% | |
| ! | TCTTTGGTTGATGTTGGAGG+TGG | + | chr7.3:75495455-75495474 | MS.gene062439:CDS | 45.0% |
| !! | ATCAAGTGGGCTGTTGAACT+TGG | + | chr7.3:75494987-75495006 | MS.gene062439:CDS | 45.0% |
| !! | TCACGGTGTCATGCGTTTAT+TGG | + | chr7.3:75495103-75495122 | MS.gene062439:CDS | 45.0% |
| !!! | TTTTATTTTTTTTTTTTGAA+GGG | + | chr7.3:75495960-75495979 | MS.gene062439:intron | 5.0% |
| !!! | TTTTTATTTTTTTTTTTTGA+AGG | + | chr7.3:75495959-75495978 | MS.gene062439:intron | 5.0% |
| AGATGAATACGTGGGTGACC+GGG | - | chr7.3:75495809-75495828 | None:intergenic | 50.0% | |
| AGATGTCGGAGTTTGAACCC+CGG | + | chr7.3:75495788-75495807 | MS.gene062439:intron | 50.0% | |
| CATGACACCGTGAACACAAC+CGG | - | chr7.3:75495096-75495115 | None:intergenic | 50.0% | |
| GAGCAAATGGACATCTACGG+AGG | + | chr7.3:75495286-75495305 | MS.gene062439:CDS | 50.0% | |
| GCAAAAGCCGGTTGTGTTCA+CGG | + | chr7.3:75495086-75495105 | MS.gene062439:CDS | 50.0% | |
| GTTGATGTTGGAGGTGGAAC+CGG | + | chr7.3:75495461-75495480 | MS.gene062439:CDS | 50.0% | |
| !! | ACACAACCGGCTTTTGCTTC+TGG | - | chr7.3:75495083-75495102 | None:intergenic | 50.0% |
| AACAGCCCACTTGATCGACG+CGG | - | chr7.3:75494982-75495001 | None:intergenic | 55.0% | |
| GATGAATACGTGGGTGACCG+GGG | - | chr7.3:75495808-75495827 | None:intergenic | 55.0% | |
| TGGAGGTGGAACCGGAAACA+CGG | + | chr7.3:75495469-75495488 | MS.gene062439:CDS | 55.0% | |
| ACAGCCCACTTGATCGACGC+GGG | - | chr7.3:75494981-75495000 | None:intergenic | 60.0% | |
| CGAAGAGCCGAGACAAGCTC+AGG | - | chr7.3:75495056-75495075 | None:intergenic | 60.0% | |
| ! | GAGACCCGCGTCGATCAAGT+GGG | + | chr7.3:75494974-75494993 | MS.gene062439:CDS | 60.0% |
| ! | TGAGACCCGCGTCGATCAAG+TGG | + | chr7.3:75494973-75494992 | MS.gene062439:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75494894 | 75496425 | 75494894 | ID=MS.gene062439 |
| chr7.3 | mRNA | 75494894 | 75496425 | 75494894 | ID=MS.gene062439.t1;Parent=MS.gene062439 |
| chr7.3 | exon | 75494894 | 75495643 | 75494894 | ID=MS.gene062439.t1.exon1;Parent=MS.gene062439.t1 |
| chr7.3 | CDS | 75494894 | 75495643 | 75494894 | ID=cds.MS.gene062439.t1;Parent=MS.gene062439.t1 |
| chr7.3 | exon | 75496123 | 75496425 | 75496123 | ID=MS.gene062439.t1.exon2;Parent=MS.gene062439.t1 |
| chr7.3 | CDS | 75496123 | 75496425 | 75496123 | ID=cds.MS.gene062439.t1;Parent=MS.gene062439.t1 |
| Gene Sequence |
| Protein sequence |
